LOCUS       DSCC01000001            1773 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045360, whole genome shotgun sequence.
ACCESSION   DSCC01000001 DSCC01000000
VERSION     DSCC01000001.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045360"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            84..1058
                     /locus_tag="ENN56_00005"
     CDS             84..1058
                     /locus_tag="ENN56_00005"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73898.1"
                     /translation="MNRTLLGLVLAGGRGTRFGNLTKRIPKPLLPVAGKPLIIRNIDN
                     LRRAGVTDITIVISPDSEQVRSVLGNGHTLGVNLEYVVQTDHLGTAHAIGLARDIIGD
                     HRFLVCWSDNLTTYALSDLIDNDRVFPADGTLAVHFTENPRKGGIVITKARTITEFEE
                     KPEKPRSNLNLAGMYILGPWVFGAIDKTRLNSKEGEYYLPDAFQIAVNDGLTLNWALV
                     GDWRMNVNTPEDLLVGNARLLENDGHTTPADGSYTIMKPVHIPSSVQIGHGATIGPYV
                     SIGEGAVIGDDARIADAVILGGAVVPDAAEIDRAIVDIDGTVYAAQGD"
     gene            1102..>1773
                     /locus_tag="ENN56_00010"
     CDS             1102..>1773
                     /locus_tag="ENN56_00010"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00288.24,HMM:PF10509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73899.1"
                     /translation="MTDEIIIAPASTWQHILSQPSDAFVAEVARVRAETPAEAKHAIG
                     WYRTLLDGAMKSHQRNPNDDVAFIRAPGRVNLLGTHIDHRGGRVNPIAVRELMLVMFP
                     RTDNRVRIANADASFAPDEFAIADLLPDGPVSDWPDWTLSTPNRLKEQGLLGTWGSYA
                     RAACAYMANAWAETDSIRGFDLYVDTQLPPSAGLSSSSALTVGSAIALHVANERTFDR
                     RELAEQ"
BASE COUNT          372 a          544 c          521 g          336 t
ORIGIN      
        1 cagtcgctcg ccgacggtgg ttcgatcatt atctgatgat aacacatcga acgtagcgtt
       61 taccgtagat aggaacgtgt tgtatgaatc gcacactact cggactcgtt ctcgcaggag
      121 gccgtggaac gcgctttggc aacctcacaa agcggatccc aaagccgctt ttaccggttg
      181 ccggtaagcc gctcatcatc cggaatatcg ataatcttcg tcgcgccggc gtgacggata
      241 ttacgattgt catctcgccg gattcggagc aggtgcgcag cgtgctcggt aatggacata
      301 cgctcggtgt gaatctcgag tacgtggtac agaccgatca tctcggcacg gcacacgcga
      361 tcgggcttgc gcgcgatatc atcggcgacc atcgctttct cgtatgctgg tcggacaacc
      421 tgacgaccta cgcgctatcg gacctcatcg ataacgaccg cgtattcccc gccgatggaa
      481 cgctcgcggt ccactttacc gagaacccgc ggaagggcgg tatcgttatc accaaggcgc
      541 gcacgatcac ggaattcgag gaaaaaccgg aaaagcctcg cagtaacctc aatctcgccg
      601 gtatgtatat cctcggaccg tgggtattcg gcgctattga caagacgcga ctcaattcga
      661 aagaaggcga atactaccta cccgacgcgt ttcaaatcgc cgtaaacgac gggctgacgc
      721 tcaattgggc gctcgttggc gactggcgca tgaacgtcaa cacgccggag gacctcctcg
      781 tcgggaacgc gcgcctcctt gagaacgacg gacatacgac accggcggac ggcagctata
      841 cgatcatgaa gccggtgcat atcccgtcga gcgtgcagat cgggcacggc gcgacgatcg
      901 ggccgtacgt aagcatcggt gaaggtgcgg tgatcggcga cgacgccaga atcgccgacg
      961 cggtaattct gggcggcgcg gtcgttcccg acgctgccga aatcgaccgc gcaatcgtcg
     1021 atatcgacgg cacggtatac gcggcacaag gcgactgatt ccacggcgat ttctcccaaa
     1081 ggcgcgtgaa aggttttccg tatgaccgac gagatcatca tcgcaccagc atcgacgtgg
     1141 cagcatattc tatcccaacc gagcgacgcg tttgtcgcgg aagttgcgcg cgtccgcgcg
     1201 gagactcccg cggaagcgaa gcacgctatt ggttggtatc gaacgctgct tgatggcgcg
     1261 atgaagtccc atcaacgcaa tcccaacgat gacgtcgcgt ttatccgcgc gcccggtaga
     1321 gtgaatctcc tcggcacaca tatcgaccac cgcggcggtc gtgtaaaccc catcgccgtg
     1381 cgcgaactca tgctcgtcat gttcccacgc accgataatc gcgtgcgaat cgccaacgcc
     1441 gatgcgagct tcgctcctga cgaattcgcc atcgccgatc tgcttcccga cggtccggtg
     1501 agcgactggc cggactggac gctctcgacg ccgaaccggc tcaaggagca gggcctgctc
     1561 gggacgtggg ggagctatgc ccgcgcggca tgtgcctata tggcgaatgc gtgggccgaa
     1621 acagactcta tccgtggctt cgacctctac gtcgatacgc agctcccgcc atcggccggg
     1681 ttgagctcgt cgtcggcgct cacggtcgga tcggcaatcg cgctgcacgt tgccaacgag
     1741 cgcacgttcg accggcgcga gcttgccgag cag
//
LOCUS       DSCC01000002            2201 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033805, whole genome shotgun sequence.
ACCESSION   DSCC01000002 DSCC01000000
VERSION     DSCC01000002.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033805"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="ENN56_00015"
     CDS             complement(<1..1878)
                     /locus_tag="ENN56_00015"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01074.20,HMM:PF07748.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73900.1"
                     /translation="MADKAAWRSEPLPDVTEYPSKEIHIISNTHWDREWVYPSAETRL
                     LLLDFMDNMLDLLDAQPEYHSFTMDSQTLCIQDYLDFRPERREQIERHVKSGRLIIGP
                     WCSLPEEYIVNGESIVRNLVIGHKVSESLGGVSKIGYTPFSYGQTSQMPQIYRGFDID
                     TIIFYRGIKTAKSEFIFRGADGSELLGMRFGALSRFSYFFYLFRMVAYNMTRDEALYE
                     WRRGSLPFKLAGDHHENAHFYICDPDDDGWWTENIESGAKRLLRDESQHFSTKYIASM
                     QGFDSSEPDPREVDLIKETQKHLPGHVIKQSDLKTFMDLLRKEYEEGRLKPEVIEGES
                     RNPNSVGKWTHLFGDVISSRVRIKLMNNANEQNVQRRAEPWSAISLSMGDAYPSTVID
                     ECWDNIMKNHPHDTICGAGVDQMEKDMIARGEQVNLFSMGLYRRALQRIQTRIDNSDL
                     AENEVGLTVFNPSPFTRSEVVSVFLDLPDDCEYEDFTIRDEKGNVCEVQDVEYFPWGI
                     LVRNLRDVSLQLHSERARLHFEAKDIPPFGYRTYVVKHEPDVVWKPMGHMVTGVNQME
                     NEFLRVKFNSDGTLRMENKETGDVFDGMHYLEDAGENGHSWMHESPNRDEIITSHAAP
                     CH"
BASE COUNT          398 a          612 c          685 g          506 t
ORIGIN      
        1 gtgacacggc gccgcgtggc tcgtgatgat ctcgtcgcgg ttgggtgatt catgcatcca
       61 cgagtggccg ttttcgccgg catcttcgag gtagtgcatg ccgtcgaaga cgtcacccgt
      121 ctccttgttc tccatgcgga gcgtaccgtc ggagttgaat ttcacacgga ggaactcgtt
      181 ctccatctgg ttcacgccgg tgaccatgtg gcccatcggc ttccagacaa catcgggctc
      241 gtgcttgacg acgtaggtcc gatagccgaa cggcgggatg tccttcgctt cgaagtgaag
      301 ccgtgcgcgt tcgctgtgaa gctggagcga gacgtcgcgc aggttacgta cgaggatacc
      361 ccacgggaaa tactcaacgt cctgcacttc gcagacgttg cccttctcgt cgcgaatcgt
      421 gaagtcttcg tattcgcagt cgtccgggag gtcgagaaat acgctgacca cctcgctgcg
      481 ggtgaacggc gacggattga acacggtcaa gccgacttcg ttctccgcca ggtcgctgtt
      541 gtcgatccgt gtttgaatgc gctggagcgc gcgacgatag aggcccatgg agaacagatt
      601 gacctgctca ccacgcgcga tcatgtcctt ctccatctga tcgacacccg cgccgcagat
      661 cgtgtcgtgc gggtggttct tcatgatgtt gtcccagcac tcgtcaatca ccgtcgaggg
      721 gtacgcgtcg cccatgctga gcgagattgc cgaccatggc tcggcgcgac gctgcacgtt
      781 ttgctcgttt gcgttgttca tgagcttgat gcgcacgcgg ctggagatca cgtcgccgaa
      841 gaggtgcgtc cacttgccga cggaattcgg attacggctt tcgccctcga tgacttcggg
      901 cttgagacga ccttcttcgt attccttgcg caggaggtcc atgaacgtct tcaggtcgct
      961 ctgcttgatg acgtggccgg gcaggtgctt ctgcgtctct ttgatgagat cgacttcgcg
     1021 cggatccggc tcggagctat cgaagccctg catcgaggcg atgtacttcg tcgagaagtg
     1081 ctgcgattca tctcggagga ggcgcttcgc accggactcg atgttctccg tccaccaacc
     1141 gtcgtcatcc gggtcgcaga tgtagaagtg cgcgttctcg tgatggtcgc cggcgagctt
     1201 gaatggcagg gaaccgcgcc gccattcgta gagcgcctcg tcgcgggtca tgttgtacgc
     1261 gaccatgcgg aagagataga agaagtagct gaaccgagag agtgcgccga agcgcatccc
     1321 gaggagctcg cttccgtcag cgccgcggaa gatgaactcg gatttggcgg ttttgattcc
     1381 gcgatagaaa atgattgtat cgatatcgaa accgcggtag atttgcggca tttggctcgt
     1441 ctggccgtag ctgaacggcg tgtatccgat cttggacaca ccgccgaggc tttccgatac
     1501 cttgtgaccg atcacgagat tgcggacgat cgactcgccg ttgacgatgt actcttccgg
     1561 cagcgaacac cacggtccga tgatgagtcg gcccgacttg acgtgtcgct cgatctgctc
     1621 gcgacgttcg ggacggaaat cgagatagtc ctggatacag agcgtctggc tatccatggt
     1681 gaacgaatgg tactcgggct gcgcgtcgag caggtcgagc atgttgtcca tgaaatcgag
     1741 gaggagaagg cgtgtctccg cgctcgggta tacccactca cgatcccagt gggtattgct
     1801 gataatgtgg atttccttgg acgggtactc cgtcacatcg ggcagcggtt cgctacgcca
     1861 tgcggctttg tcggccatgg gcggtgctcc tttcgggaat gtcggcgatc tcgtagactc
     1921 gacgcgagta gacgatatgt cgaactcgtc tactcgcgcg gtacgttcag tttgtcgttg
     1981 tttgataggt ttgcacgcag tgcgttcgga tacgcgcacg gcaatatgtc aatatagcgt
     2041 acgcgcgcct gcgaacgaag gtggaaagct tcggattact ccgtcgcggc ttccggcgcg
     2101 tgaatcggct gatcggcgat tgtgccgtcg gcgagtcgga tgtaggtctg ccatgcgcgc
     2161 ccctcgtcgg tcagcacaat cacccgtgcg ccgcgtggga a
//
LOCUS       DSCC01000003            2424 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029665, whole genome shotgun sequence.
ACCESSION   DSCC01000003 DSCC01000000
VERSION     DSCC01000003.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029665"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1787
                     /locus_tag="ENN56_00020"
     CDS             <1..1787
                     /locus_tag="ENN56_00020"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13432.4,HMM:PF13525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS73901.1"
                     /translation="AYQGFFDRYPNSDRRDFVLVRLAECAVRGAAQGVGNPQQALRQF
                     DQAIEFAPSGPVLDRALFGSALILRGQSQTDEAKRRFQRLIAERPLSNLVPESRYQLA
                     MILLDENQPNAAARELEKLLRSGDRSRDINDIRVRLISAYEKTGSYQQILPVARDIRD
                     SDNPSAAAQGARLLARALVELRHLADAEAVLREELSTRPDAPDADSLTIRHAEILIKI
                     NRIDLIGGVLGGFERRFPRSTRVPVAWRMLAHVQFDLNAYEEALASYRRVLQADGRDK
                     EARLGEIVALYRLGRANEAQQRERELRDMTRLTADDEVRLSLEEGHALFRARDFMAAI
                     EAFAEVVKNHPESEWADDALLAQGRAGASSGRIEPAVAAFEKLIRDYPDSPLRQEAAF
                     ELGNAYFGARFYEQSSESYQRALEIDTTSRFAADALWNLVVSYEALQRFDSAIRTMRQ
                     FLIKYPDHERAPRALVKIAEDLNRLGEFEDAVAAYHEALERVSGEDVAAARFGLGEAY
                     FNLGEYRLAMVEWLKMAYHGQTQSSWAITALYRAAKANEKMGRVEDAATLYRKIVTIE
                     GENSDWGRSAAMELIRLNQSTEGEEASP"
     gene            1807..>2424
                     /locus_tag="ENN56_00025"
     CDS             1807..>2424
                     /locus_tag="ENN56_00025"
                     /inference="COORDINATES: protein motif:HMM:PF00027.27"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDS73902.1"
                     /translation="MRDIKELLRGVPFFEDLTENDLTRVSALVVTKVYPPDNLIVLAE
                     DEGDTLFIIVRGQVKVSIMSEDGREVILSMLGPGDFFGEMSLLDGKPRSATVTAVVET
                     ELISLRRSEFLRLLEQVPEIALKLLAGLTARLRQADRKIESLALMDVSGRIAAAILQM
                     SEEIGQETPHSIVIRNRPTHQSLANTAGTTRETVSRVLKRFEHQKY"
BASE COUNT          562 a          655 c          712 g          495 t
ORIGIN      
        1 acgcgtacca aggcttcttc gatcggtatc cgaacagcga tcgacgcgat ttcgtgcttg
       61 tacgacttgc cgagtgcgcg gttcgtggcg ccgctcaagg cgtcgggaac ccgcaacaag
      121 cgttgcgtca gtttgatcag gcaatcgaat tcgcaccgag cggcccggta ctcgatcgtg
      181 cgctttttgg ttccgcactc attcttcgcg gtcaaagtca aacagacgaa gcgaagcggc
      241 ggtttcagcg tttgattgcc gaacgtccgt tgagtaacct ggtgccggaa agtcggtacc
      301 aacttgcgat gattctactt gacgagaatc aaccgaatgc agccgcacgg gaactcgaaa
      361 agctccttcg aagtggtgac aggtcgcgcg acatcaatga tatacgagtg cgattgatct
      421 cggcatatga aaagacggga tcgtatcagc agattctccc tgtcgcgcgg gacatacgtg
      481 attcggataa tccctcggcg gcggcgcagg gagctcgcct tctcgcacgc gcgcttgttg
      541 agcttcgcca ccttgcggac gctgaagcgg tgctgcgcga agaattaagt acccgacccg
      601 acgcaccgga tgcagatagc ctcacgattc gacatgcaga aatccttatc aagatcaatc
      661 gcatcgatct aatcggcggc gttctcggcg gtttcgagcg tcgattcccc cgcagcacac
      721 gcgtgcccgt ggcgtggcgg atgctcgcgc atgtgcagtt cgatctcaat gcgtacgaag
      781 aagcgcttgc gagttaccgc cgcgtgcttc aagctgacgg tcgggataag gaggctcgcc
      841 ttggcgagat agtcgcgttg tatcgactcg gacgtgccaa cgaagcgcag cagcgcgaac
      901 gtgagctccg cgacatgacc agactgacgg ctgacgatga agtccgcctc tcactcgaag
      961 aggggcacgc actgttccga gcacgagatt tcatggccgc aatcgaggcg tttgccgagg
     1021 ttgtcaagaa tcaccccgaa agcgaatggg ccgatgacgc gctattggct caaggtaggg
     1081 cgggagcgag tagcggacgg atcgaacctg cggtagccgc gttcgaaaag ttgatccgtg
     1141 attaccccga tagtccgtta cgtcaggaag ctgcgttcga actcggaaac gcgtatttcg
     1201 gtgcgcgatt ctacgaacaa tcgtcggaga gttaccaacg cgcgctcgaa atcgatacga
     1261 cgagtcgctt tgccgccgat gctctctgga acctcgtcgt gtcgtatgag gcactccagc
     1321 gttttgactc ggcgattcgg acgatgcgtc agttccttat aaaatacccg gatcacgagc
     1381 gcgcccctcg tgcgcttgtg aagattgcag aagatctcaa tcggctcggc gagttcgagg
     1441 atgccgtggc ggcgtatcac gaagcgcttg aacgggtttc cggtgaagat gtcgcggcag
     1501 cgcgattcgg tcttggagaa gcgtatttta accttggaga gtatcgctta gccatggttg
     1561 aatggttaaa aatggcatac cacgggcaga cacagtcaag ttgggcgatt acggcgctct
     1621 atcgtgccgc gaaagcaaat gaaaaaatgg gacgtgtcga agatgccgct acgctctacc
     1681 ggaagatcgt caccatcgaa ggagaaaaca gcgactgggg acgatcggcg gcgatggaac
     1741 tgatccgtct gaaccaatcc accgagggtg aagaagcctc accataggcg gacggagaaa
     1801 ccaacgatga gagatatcaa agagctactc cgcggggtac cgttttttga agacctcacg
     1861 gaaaacgatc tcacgcgagt gagtgcgctc gtcgtaacga aagtctatcc gcccgacaat
     1921 cttatcgtgc tcgcggaaga cgagggcgac acgttgttta tcatcgtccg aggtcaggta
     1981 aaagtctcga tcatgagtga agacgggcga gaggtaatac tctctatgct tggtccggga
     2041 gacttcttcg gcgaaatgtc tctcctcgac ggaaaacctc gttctgcaac cgtaaccgca
     2101 gtcgtagaaa ccgagttgat ttcgctccgt cgttcggagt ttctccgcct cctcgaacag
     2161 gttcccgaga tcgcgctcaa gctcctcgcc ggactcacgg cgcggttgcg gcaggccgac
     2221 cgaaaaatcg aaagcctcgc gctcatggat gtcagcggac gtatcgcggc tgcgatactg
     2281 cagatgtctg aggaaatcgg ccaagagaca ccacacagta tcgtgatccg aaatcgtccg
     2341 acgcatcagt ctctggcgaa cacggcagga acaacgcgcg agacggtatc tcgcgttctc
     2401 aaacgattcg aacatcagaa gtac
//
LOCUS       DSCC01000004            1853 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042784, whole genome shotgun sequence.
ACCESSION   DSCC01000004 DSCC01000000
VERSION     DSCC01000004.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042784"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..760)
                     /locus_tag="ENN56_00030"
     CDS             complement(<1..760)
                     /locus_tag="ENN56_00030"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73903.1"
                     /translation="MNRTQMKSSRKKPSRFPDRIESLPWNWIVTGSYAVVMGVISFTM
                     WPVGGYGVETDAFAGYLPDAAKLLNGSFTTLDGFKGPGYHIAVAAVGLIVRDLFLAAK
                     LIAIVSASAVLLLTFRLVRAFLGAKAAWITFAVIATNAQFALYTIQVGTDVYFLALAV
                     AAGYFILDRPGVSRTRHVRRAFIAGAIGGLAYLTRYNGLFLLLGGTIVYLLADWKETP
                     EYSETTDEQPLPWRTRVYRTIAFVFAAAITILPWS"
     gene            1139..>1853
                     /locus_tag="ENN56_00035"
     CDS             1139..>1853
                     /locus_tag="ENN56_00035"
                     /inference="COORDINATES: protein motif:HMM:PF01558.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73904.1"
                     /translation="MSYQLPYKDRLGFCNVLMSAIGGDGANMAAKLLFKIAVSELNLD
                     GAYDAKYGSEKKGTPTDVSLRLCDPGTPVRTSGPTDSPHILAIFRPGLITALHLNRGL
                     QDNATVIVNTTETPDQIREKLKLHSGTILTVDAEMIAEESHSRLNMPMMALIARVLGF
                     PSEILAQQVEKQWPFAAKANLAAFNAAYDKILEKKFEARDDFPLVPPGSVTRGEIGYT
                     NMLNGGAINALDHSTVNRNN"
BASE COUNT          437 a          499 c          537 g          380 t
ORIGIN      
        1 acgaccacgg cagaatcgtg atcgcggcgg cgaatacgaa cgcaatcgtc cggtacacac
       61 gtgttcgcca cgggagcggc tgttcgtcgg tggtctcgga atactcaggg gtctccttcc
      121 agtcggcgag aaggtatacg atcgttccac cgaggagcaa aaacagcccg ttataccgcg
      181 tgagatacgc caagccgcca atcgctccgg cgatgaacgc gcggcgaaca tggcgagttc
      241 gggatacgcc cggacgatca agtatgaagt atccggcggc tacggcgagt gcgaggaagt
      301 acacgtcggt accgacttga atggtgtaaa gtgcgaactg cgcgttcgtc gcgattactg
      361 cgaaagtgat ccatgcggcc ttcgctccaa gaaatgcacg aacgaggcgg aaggtgagaa
      421 gtaaaacggc ggaggcactg acgatggcga tgagtttcgc tgcgaggaag agatcgcgaa
      481 cgatgagacc gacggcagct accgcgatat ggtatccagg ccccttgaat ccatcgagtg
      541 tcgtaaacga accgttgagg agcttcgccg cgtctggaag gtaacctgcg aacgcgtccg
      601 tttcaacgcc atacccgcca actggccaca ttgtgaagct gatgacgccc atcaccacag
      661 cgtacgagcc agtgacaatc cagttccacg gtaacgactc gattcgatca gggaagcggc
      721 tcggtttctt cctcgacgat ttcatctgcg ttcgattcat ggtagtttac tctttctgcg
      781 gcaccatacc tacggaaaag gatcacggtg taagatgcag ccgtcgcaac gcacaagcaa
      841 acgaccggag cactcgcctt cgggtcgatt cgacaggaga gactcggtaa cgcgacgcgt
      901 cttcgtatgt gccaattgtc agcatcgatt tgacaaaatg tctcaccatt gtgggtcgcg
      961 cgcgtgattc atatgatcgg agagaacacg ggtacaggta gttagcgtaa cgcatcggtg
     1021 taaaggcggg tgttggcata gaggttgcac aaaaaggggg tgtcttccgg acaaccgcgc
     1081 gaaatgcgct attcgaccac ctgccggtct tatttgtttc ccctaaggag ggacatacat
     1141 gtcctatcaa ctcccgtata aggatcggct tggattctgc aatgtgctca tgtctgccat
     1201 cggtggcgac ggagcgaaca tggcggccaa gcttctgttc aagatcgccg tgagcgaact
     1261 caacctcgac ggagcgtatg acgcgaagta cggttcggaa aagaagggta ctccgacgga
     1321 tgtatcactg cggctctgcg atccgggcac gccggtacgc acctccggtc cgacggattc
     1381 gccgcatatc ctcgcgattt tccgtccggg tctcattact gcgctacatc tcaaccgcgg
     1441 tcttcaggac aacgcgacgg tcatcgtgaa tacgacagaa acgcctgatc agattcgtga
     1501 gaagctcaag ctacacagcg gtacgattct taccgttgac gccgagatga ttgcggagga
     1561 atcccactcg cgtttaaaca tgccgatgat ggcgcttatc gccagggtgc ttggtttccc
     1621 gagtgaaatc ctcgctcagc aggttgagaa gcagtggccg ttcgccgcga aggcgaatct
     1681 cgctgcgttc aacgccgcgt atgacaagat tctcgagaag aaattcgaag cgcgcgatga
     1741 tttcccgctc gtcccaccgg gatcggttac tcgtggtgag atcgggtaca cgaacatgct
     1801 caatggcggt gcgatcaatg cactcgatca ttcgaccgtg aatcggaaca atc
//
LOCUS       DSCC01000005            1863 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042423, whole genome shotgun sequence.
ACCESSION   DSCC01000005 DSCC01000000
VERSION     DSCC01000005.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042423"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            95..1066
                     /locus_tag="ENN56_00040"
     CDS             95..1066
                     /locus_tag="ENN56_00040"
                     /inference="COORDINATES: protein motif:HMM:PF00384.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73905.1"
                     /translation="MGNANEIDIIPGHVIDNPMATNIVDLCPVGALLTEDFLFKARVW
                     NLKPMPSIDPSDSLGANTYLDVMNNEVQRTRPRENTAVNGYFMTDEGRFMYHVIRSEQ
                     RLVTPVQPDPESGELLEAPWEPALEFIDGKMRVAGSNAVVLMSTHVTQEEVALAKEYA
                     AAIGTDKIAYIPNALVTDDQTFPGGYVISGDKSPNTQGVTQELPSSVDDVDITGESVV
                     LVINSSVRSENVSDAHLGKILGADFVFTIDVLKSPLVKRAFLSLPGRMWAEKSGTWIN
                     RSGITQEFSPAVVGPVGSRDERDLLRELTNRAKKPRVNQTQAERVTT"
     gene            1134..>1863
                     /locus_tag="ENN56_00045"
     CDS             1134..>1863
                     /locus_tag="ENN56_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HDS73906.1"
                     /translation="MLLAMLAIAGLYTFYERKVAAWMQNRYGPNRVGPKGYFQFMADG
                     IKFFMKEDVVPAYANKPIFLLAPVMIMVPALLLFTAVPLGMPFTWNGYDINLKITNFD
                     IGILFVLAVAGMGVYGIVLGAWASNSKYPLFGGLRASAQMVSYELAMGLSVVAVVLIA
                     GGLSLTTIIGQQTEYWFGFIPKWNILANPLAFLIFLIATYAETNRLPFDFAEAEQELV
                     GGYHTEYSSMKFAVFMLSEYVNMIT"
BASE COUNT          427 a          473 c          529 g          434 t
ORIGIN      
        1 aatccggctg tataccaacc gatgcattct ctgcgaccgc tgcgttcgct ttctccgtga
       61 tttcgtgggc actggcgaat taaccatacg gaacatggga aacgcgaacg agatcgacat
      121 catacccggt cacgtgatcg acaatccgat ggcgacgaat atcgtcgatc tctgtccggt
      181 tggtgcactg ttgacagagg attttctctt caaagcgcgc gtctggaacc tcaagccgat
      241 gccgtccatc gatccttcgg attcactcgg tgcgaacacc tacctcgatg tcatgaataa
      301 cgaagtccaa cgcactcgtc cgcgcgagaa cacggcggta aacggatact tcatgaccga
      361 tgaaggccgt ttcatgtacc acgtgatacg ctctgagcag cgtctcgtga cgccggtgca
      421 gcccgatcct gagtcgggtg agttgctgga agcgccgtgg gaaccggcgt tggagttcat
      481 cgacggaaaa atgcgcgtgg cagggtcgaa cgcggtagtt ctcatgtcaa cgcacgttac
      541 gcaagaagaa gtcgcgttgg cgaaggaata tgccgcggcg attggcacgg ataagatcgc
      601 gtacatccca aacgcgttgg tgaccgacga tcagacattc cccggtggct acgtgatttc
      661 gggcgacaag tcgccgaata cacagggtgt cactcaggaa ctaccatcca gtgtcgatga
      721 tgtcgacatt acgggcgaat cggttgttct cgtaatcaac tcatccgtgc ggtcagagaa
      781 cgtctccgac gcacatttgg gcaagatact tggtgccgat ttcgttttta caatcgacgt
      841 gttgaagagc ccgctcgtga agcgtgcgtt tctctcactg ccgggacgta tgtgggccga
      901 gaaatcaggt acgtggatca atcgtagcgg tatcacgcag gagttcagtc cggcagtggt
      961 cggaccggtc ggatcgcgag atgagcgaga tctgctacgc gaattgacga atcgagcgaa
     1021 aaaaccgcga gtcaatcaga cacaagccga acgcgtgacg acgtaataga tcgggagttc
     1081 gaagcgtgat ctcaacacca caattgatcg tatcgctcgt cctcggactc gtcatgctcc
     1141 ttgcgatgct ggcgatagcc ggtctctaca cgttctacga gcgcaaagtt gccgcatgga
     1201 tgcagaaccg gtacgggccg aaccgcgtcg ggccaaaggg ctatttccag tttatggcgg
     1261 atggaatcaa gttcttcatg aaggaagacg tggttccggc gtatgcgaac aaaccgatct
     1321 ttcttctcgc gccggtgatg atcatggttc cggcgttgct tttgtttacc gccgttccgc
     1381 ttggaatgcc gttcacgtgg aacggatatg atatcaatct caagatcacg aatttcgaca
     1441 tcggcattct cttcgttctt gccgtcgccg gaatgggtgt gtatggtatc gtgctcggtg
     1501 cgtgggcgtc gaacagcaaa tatccgttgt tcggcggatt gcgcgcgtcg gcgcagatgg
     1561 taagttacga gctcgcgatg ggattgagcg tcgtggcggt agtactcatt gccggcggat
     1621 tgagcctgac aacgattatt ggacagcaga cggagtattg gtttgggttt attccgaaat
     1681 ggaacattct cgccaatcca ctcgcgtttc tcatatttct catagcgacg tacgccgaaa
     1741 caaatcgctt accattcgat tttgcagagg ccgaacagga gctcgtcggc ggataccata
     1801 ccgaatacag ctcaatgaag tttgcggtat tcatgttgag cgaatacgtg aacatgataa
     1861 cca
//
LOCUS       DSCC01000006            1572 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053377, whole genome shotgun sequence.
ACCESSION   DSCC01000006 DSCC01000000
VERSION     DSCC01000006.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053377"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..357
                     /locus_tag="ENN56_00050"
     CDS             <1..357
                     /locus_tag="ENN56_00050"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS73907.1"
                     /translation="VLVVDDAPTLRTYISEVLKRAGYDVTTATDGADALEKLREVAVD
                     LVISDLEMPRMNGDELVVEIKKREDMRTLPVILLTAQTDAKHRRAAAALGVAGYLTKP
                     FDEARLLRAVSELVVS"
     gene            354..866
                     /locus_tag="ENN56_00055"
     CDS             354..866
                     /locus_tag="ENN56_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73908.1"
                     /translation="MSAKSSIRDTAIRFVLLRCTVGDMEFGVEIGAVLRVEGHLHIGG
                     NCGAVDEPYGIHGGVYGIDTARLFLGEQTHTWSDAAKDIVLLYQNQTFAMTADSVIGL
                     YTARVDELTTWYDPPDHPATPFVRAFLPMADGILYVVNLRSVLRYIDTIDGEGDVTDE
                     ASMERSEFNG"
     gene            859..>1572
                     /locus_tag="ENN56_00060"
     CDS             859..>1572
                     /locus_tag="ENN56_00060"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS73909.1"
                     /translation="MAEEQKILVVDDEDVITWAISQGLTRRGELAVDTASSGEEALGI
                     LEQRTYDLVITDIRMRGMNGFELLTHIRSRYPDTGVVVMTAYGSAEAKREASERGSLF
                     YLDKTFEMDEMRQVVRSALEQVERARALRDQQSEGFSGVISDLSLVDMVQLHCLARNN
                     ALMEVTTPRRQGVIGFRDGEIVFASTDAGLEGRDAFLDMLSWSGGEFETLNKNPTEQN
                     IDESWESLLLEASDIIANQN"
BASE COUNT          353 a          390 c          486 g          343 t
ORIGIN      
        1 gtgctcgttg tcgatgatgc gccgacactc cgcacgtaca tttcggaagt gctgaaacgc
       61 gccgggtacg acgtgacaac ggcgactgac ggagccgatg cgttggagaa gcttcgggaa
      121 gtcgcagttg atcttgtgat cagcgatctc gagatgccac gtatgaacgg tgacgagctc
      181 gtcgtcgaaa taaaaaaacg tgaggatatg cgaacgcttc ctgtgatcct gctgaccgcg
      241 caaacggacg caaaacatcg tcgcgctgca gccgcgctcg gggtggcggg gtatctcacg
      301 aaaccgtttg acgaagcccg gttacttcgc gccgtatcgg agttggtggt atcatgagtg
      361 caaagtcgtc catacgcgat accgcgatac ggtttgtttt actccgctgc acggtcggcg
      421 atatggagtt tggtgtcgaa atcggagccg tgctacgtgt tgagggacac ctacacatcg
      481 gtggtaactg tggagcagtg gacgagccgt acggcattca tggcggcgtg tatggtatcg
      541 acacggcgcg gctcttcctc ggtgaacaaa cgcatacatg gagcgacgcc gcgaaagaca
      601 tcgtactgtt gtatcagaac cagacattcg caatgaccgc ggattcggtt attggtctct
      661 acaccgcgcg cgtcgatgaa ctgactacgt ggtacgatcc tcccgaccat ccggcaaccc
      721 cgtttgttcg ggcgtttctt ccgatggcgg acggtattct gtatgtggtg aacctccgga
      781 gtgtactccg gtatatcgat acgattgacg gtgaaggtga cgtcactgac gaggcgagta
      841 tggagcggag cgaattcaat ggctgaggag caaaagattc tcgttgtcga cgacgaggac
      901 gtgatcacgt gggcgatctc acagggactg acgcgacgtg gcgagcttgc cgtagatacg
      961 gcatcgagcg gcgaggaggc tctcggcatc cttgaacagc gtacctatga tcttgtgatt
     1021 accgatatcc gtatgcgcgg aatgaatggc ttcgagctcc ttacgcacat tcggtcacgg
     1081 tatccggaca ccggtgtcgt cgtaatgact gcgtacggat ccgcagaggc gaaacgggaa
     1141 gcatcagaac gtggatcgtt gttctatctc gataagacct ttgagatgga cgaaatgcgg
     1201 caggtcgttc gatccgcact tgagcaagtc gaacgagctc gcgcactacg cgatcaacag
     1261 tcggagggat tcagtggcgt tatttccgat ttgagccttg tcgacatggt tcagcttcac
     1321 tgcctcgcgc gaaacaacgc gttgatggaa gttaccacgc cgcgaaggca gggcgtgatc
     1381 ggctttcgag acggggagat cgttttcgcg tcgacggatg caggcttgga gggacgtgac
     1441 gcctttctcg atatgctgtc atggtcgggt ggcgaattcg agacgttgaa caagaatccc
     1501 actgagcaaa acatcgacga aagctgggaa tcgttactgc tcgaagcgag cgacatcatc
     1561 gcgaatcaga ac
//
LOCUS       DSCC01000007            1538 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054959, whole genome shotgun sequence.
ACCESSION   DSCC01000007 DSCC01000000
VERSION     DSCC01000007.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054959"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            53..637
                     /locus_tag="ENN56_00065"
     CDS             53..637
                     /locus_tag="ENN56_00065"
                     /inference="COORDINATES: protein motif:HMM:PF13458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73910.1"
                     /translation="MPTELDTMKIMREASTIDVLLVASNNTRDIVNIAAQIPTQKIWA
                     RILGGSAWGEQRVRQEAGEDAEGVIFATSFDPNGPESRRFVDTFRLARRENPSNITAL
                     AYDATSLVSEAITNGARTRGQIREYIATINGKHGVSGEISFDEAGANTDANLRLIRGG
                     IVNTITDWSTLERPSTWGVSVPKYGADTPNGDAR"
     gene            672..>1538
                     /gene="larE"
                     /locus_tag="ENN56_00070"
     CDS             672..>1538
                     /gene="larE"
                     /locus_tag="ENN56_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_951594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDS73911.1"
                     /translation="MWENVDTQTEHKQLKENLRNLGSVVVAYSGGVDSALLLAVAHKT
                     LGSDHCLAVTGISPSLAGEEYDDARRVAEQIGARWITVETNEISDLDYRRNDENRCYY
                     CKSELFDQLRKVADEYGLATIVDGYNADDVGDWRPGERAARERDVHSPLKDAGLGKEA
                     IRALSREYGLPTADKPSFACLASRLPYGTEVTPERLELIARAETTLREVGFGVFRVRY
                     HGPVARLELGPEEIERIQDRKLSSIVNDRLRAHGFEHVTVDLAGFRSGSQNESAQSDD
                     SLVGIGSVTNVLK"
BASE COUNT          373 a          407 c          439 g          319 t
ORIGIN      
        1 cctcagtgat accttgatat ccgaagaaac acgacggcgt ctcgctctga acatgccaac
       61 cgaattggat accatgaaga tcatgcggga agcaagcact attgacgttc tcttggtcgc
      121 ttcgaacaac acgcgagata tcgtcaatat tgcggcgcaa atcccaacgc agaagatttg
      181 ggcgcgaatc cttggtggtt ccgcgtgggg tgaacaacgt gttcgacaag aagccggtga
      241 ggatgccgaa ggcgtgattt tcgccacaag tttcgacccg aatggcccgg aatcacgtcg
      301 gtttgtcgat actttccgtc tcgcgcgacg ggaaaatccg tccaatatta cggcgctcgc
      361 atacgatgca acgagccttg tctccgaagc gataacgaat ggtgcgcgca cacgcggtca
      421 gatccgcgag tatattgcaa cgatcaacgg gaaacatgga gtaagtggag aaatctcatt
      481 cgacgaggct ggtgcgaata ccgacgcgaa tctgcgattg attcgcggcg ggattgtcaa
      541 cacaatcacc gattggtcaa cgctcgaacg tccgagcaca tggggcgtca gtgtcccaaa
      601 gtacggtgcg gatacgccaa atggcgacgc gcgatagagt acgagtattc caagtacacg
      661 atgccgggga aatgtgggaa aacgtggata cgcagaccga gcacaaacag cttaaggaaa
      721 accttcggaa tcttggatca gtcgtagttg cgtactcagg cggtgtcgat agcgcgcttc
      781 tactcgctgt tgctcataaa actctcggat cggaccactg ccttgctgtc acgggcattt
      841 ctccgtcgct cgcgggtgag gagtacgacg acgctcgccg ggtcgctgaa cagattggcg
      901 cgcgttggat cactgtagag accaacgaga taagcgactt ggattaccga cgcaacgacg
      961 aaaaccggtg ttattactgc aagagcgaac tgttcgacca acttcgcaag gttgcggacg
     1021 aatacggtct tgccacaatt gtcgacgggt acaacgctga tgatgtcggc gactggagac
     1081 cgggcgagcg agccgcacgt gaacgcgatg ttcatagccc cctcaaggac gccggtcttg
     1141 gaaaagaagc gattcgcgcg ctctcacgtg agtacggcct gcctacagct gacaagcctt
     1201 cgttcgcgtg tctcgcctca agacttccct acggaaccga agtaactccc gagcgactcg
     1261 aacttatcgc gcgtgccgag acgactcttc gcgaggtcgg tttcggtgtc ttccgtgtgc
     1321 ggtatcacgg tccagtagcg cgactcgaac ttgggcctga agagatcgaa cggattcaag
     1381 atcgaaagct atcctctatt gtaaacgatc gactgcgagc acacggattc gagcacgtca
     1441 ccgtcgacct tgccggtttt cgttcgggaa gccagaacga gtccgctcag agtgatgatt
     1501 cgctcgtcgg aatcggttcg gtcacaaacg tcttgaag
//
LOCUS       DSCC01000008            1546 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054590, whole genome shotgun sequence.
ACCESSION   DSCC01000008 DSCC01000000
VERSION     DSCC01000008.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054590"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            499..>1546
                     /locus_tag="ENN56_00075"
     CDS             499..>1546
                     /locus_tag="ENN56_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73912.1"
                     /translation="MARETTIDAVIAALGDSMDYAIPDHSFSPFYSDIGLGFVESNSS
                     DDEATGIDALYLHYNRATTRFGAPFVRFISDEPLVQIKRTALYVEHRFAKGNVRTSFT
                     ARNTFLISCTATDGITFRFATAGVLPGEARVTSLDGCYLFEGYLSKADQRDPDPVFPI
                     IIGLRVVCGNVSGDGVNDTLHVSPDDGKVVVAFSVNVLDIDRRDTMRLLKNVPADVGE
                     AVEMTRVWLETTLGRLDVSPLGLTDDATAEKHVLARAAYSMAANCTLAPGMLRGRVAL
                     WPARGSYPIIAPWDACFHTLGLAYMEPRLAPDQVYILTENGIRADGKHACFIASTWRR
                     PELAQPALAGWAAER"
BASE COUNT          317 a          443 c          453 g          333 t
ORIGIN      
        1 cggcctacct ttcagagata tcgttgttgg aggcgacggg tagccgcttg cgaaaacagt
       61 cgtgtaagtg cacagcgatg taacccggtt ctcaagcgaa gcggaagcgg tgtatttgca
      121 cggaacgcgt cggctgcgcg tttctcggga aacgaccttg ctgttcgtgc ggatgcatgc
      181 caacgtaaac gctcgtgcgg aaagtcggaa agagaaggaa acactttacg caggggaatc
      241 atcgcaaacg acctgaacca aatgcggtcg gatggtgtcc gcacgcggtg cattcgcaac
      301 ccctgcaaca ttttaatata ccaccggtcg taccgcgata caagtgaacg tatgatgcca
      361 tcgcgcggta tccattcaca gtcattcgca cgagtcgcgt ttcttcgaga ggcgtcgtcg
      421 gttggttcac cgcgtcttcc gtgacattct caagtcccgt aataccgttc agtcgagata
      481 attctggatt gggaaaccgt ggcaagagaa acgaccattg atgccgttat cgctgcgctt
      541 ggggattcga tggactatgc gatacccgat cacagcttta gcccgttcta cagtgacatc
      601 ggcctcggct tcgtcgagtc gaactcgagt gacgatgaag caaccggaat cgacgcacta
      661 tacctccact acaaccgtgc gacgacacgg ttcggtgcgc cttttgtgcg attcatcagt
      721 gacgaaccgc tggtccagat caagcgaacc gctctttatg tcgagcatcg gttcgccaag
      781 ggcaacgtcc gcacgtcgtt tacggcccgc aacacgtttc tcatttcgtg caccgctacc
      841 gacggcataa cgtttcgatt cgccactgcg ggcgtgcttc ccggcgaagc gcgggtaact
      901 tcgcttgacg gttgctatct cttcgaggga tacctttcga aagccgatca gcgcgatccg
      961 gatccggtat ttccgatcat catcgggctt cgtgtggtat gtggaaatgt gtccggggac
     1021 ggcgtgaacg acacgttgca cgtgtcgccg gacgatggga aggtcgttgt tgcgttctcg
     1081 gtgaatgtat tggacatcga tcgacgcgac accatgcggc tcctcaaaaa cgttccggcc
     1141 gatgtaggcg aagctgttga gatgacgcgc gtgtggctcg aaacgacgct cggacgactc
     1201 gatgtatcac cgctcggtct gactgacgat gcaaccgccg aaaaacacgt gctcgcgcgt
     1261 gcggcgtact ccatggcggc gaactgcacg ctcgctccgg gaatgcttcg cggacgtgtg
     1321 gcgctttggc ccgcgcgagg ttcgtacccc atcatcgcgc cgtgggacgc gtgcttccac
     1381 acgcttggtc tcgcgtatat ggaaccgcgg ctcgcgccgg atcaggtcta catcctcacc
     1441 gaaaacggta tccgcgcaga cggcaagcac gcgtgtttca tcgcatcgac gtggcgacgc
     1501 ccggaactcg cgcagccggc ccttgccgga tgggctgccg agcgac
//
LOCUS       DSCC01000009            1608 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1051837, whole genome shotgun sequence.
ACCESSION   DSCC01000009 DSCC01000000
VERSION     DSCC01000009.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1051837"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="ENN56_00080"
     CDS             complement(<1..1333)
                     /locus_tag="ENN56_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73913.1"
                     /translation="MSVALIAALGFATVVRASETVIERDVTWHVTQSDETFALDIAGD
                     LDRANTWYIVPYTIQHPRFDVSRSMRLENRGDNILVNPRIRANGRSDWWDINDGLADL
                     IDPDMTDREKALAIGWHILRHRYHTYGTTRETIDPVKYLNVFGYGWCTESAQALVNLW
                     KKAGLQARFYRIWGHDVSEAWYEGGWHLFDADGEVFIDVPDRIPGIGIEDIANDHALA
                     LFVPPYGSGDPWLNSSIMAQRYAAIFGGRQTSDVTFGIAFVTEHTMALRLRPDESFTW
                     YWTPQARYFGPTDYGGYARVPEYYGTGRFEFAPDFTSSRWRTGATEASNVKSRSEDGL
                     PGPLLHVAVNGQPGVLRYDFESPYVFVDGMLTGIVSVSNGGSVVCTVERFRDTVDSVE
                     HTFNTSGTYPFSIPLVDLIAPTTRSASYSIAARFEIIGRDSGLDSLAVVLDT"
BASE COUNT          318 a          436 c          522 g          332 t
ORIGIN      
        1 gcgtatcgag cacgactgcg agcgaatcga gaccgctatc gcgtccgatg atctcgaatc
       61 gcgccgctat actgtatgaa gcggaccgcg tcgttggggc gatgagatca acgagcggaa
      121 ttgaaaacgg gtaggtgccg gatgtattga acgtgtgctc gactgaatcg acggtatcac
      181 ggaaacgctc gacggtacat accacggagc cgccattgct cacggaaacg attccggtca
      241 gcattccgtc gacgaacacg tacgggctct cgaagtcgta tcgcaggacg cccggttgtc
      301 cgttgaccgc gacgtggagc agtggaccgg gaaggccatc ttccgagcgt gactttacgt
      361 tgctcgcttc ggtcgcacct gttcgccagc gagatgaggt gaagtcgggt gcgaattcga
      421 aacgaccggt gccgtagtat tccggtacgc gcgcgtatcc tccgtaatcg gtcggtccga
      481 agtaccgcgc ttgcggagtc caataccacg taaacgactc gtcggggcgc aaccgtagcg
      541 ccatggtgtg ctcggtaacg aacgcaatcc cgaacgttac gtccgacgtt tgacgtccgc
      601 cgaagatcgc cgcgtatcgc tgcgccatga tcgaactgtt taaccatgga tctcccgacc
      661 cgtatggcgg gacaaacagc gcaagcgcgt ggtcgttcgc gatgtcctcg ataccgatgc
      721 ccgggatacg gtccggcaca tcgatgaaca cttcgccatc cgcgtcgaag aggtgccaac
      781 cgccctcata ccacgcctcg gatacgtcgt gtccccagat gcggtagaat cgggcttgga
      841 ggccggcctt cttccagaga ttgacgagcg cctgcgcgga ttcggtgcac cagccgtacc
      901 cgaagacgtt gaggtatttt accggatcaa ttgtctcgcg cgtcgtcccg tatgtgtgat
      961 accgatgtcg tagaatgtgc cagccgatgg cgagggcctt ctcgcggtcg gtcatatcgg
     1021 gatcgatgag gtcggcgagc ccgtcgttga tatcccacca atccgaccgt ccgttggcgc
     1081 gtatgcgcgg gttcacgagg atgttgtcgc cgcggttttc gaggcgcatg gagcgactga
     1141 cgtcgaagcg tggatgctgg atcgtatagg ggacgatgta ccacgtattc gcgcggtcga
     1201 ggtcgccggc gatgtcgaga gcgaacgtct cgtctgactg ggtgacgtgc catgtgacat
     1261 cgcgctcgat gacggtttcg gaggcgcgga cgactgttgc gaaaccgagc gccgcgatga
     1321 gcgcaacgga cacggtggcg atccggtagt ggttgctgag gacgtgtgta gatgtggttg
     1381 tgtgcgtcgg atatgtcatg attcgtctgt cagtgcgttg tgtcagggtg aaagtgatac
     1441 gttggaataa agatacggcg aactcgtcgc gggtgcgacg agttcgttga ggcgaagctt
     1501 cgcttcgctg aacgtcttca agcgtagcga ggagacgcgg gcggtttcat tcgcaacgcg
     1561 aaacaaacgg cgcgagaccg tcatccgcgt ttcggcgaag gtacagcc
//
LOCUS       DSCC01000010            1717 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047381, whole genome shotgun sequence.
ACCESSION   DSCC01000010 DSCC01000000
VERSION     DSCC01000010.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047381"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            15..>1717
                     /locus_tag="ENN56_00085"
     CDS             15..>1717
                     /locus_tag="ENN56_00085"
                     /inference="COORDINATES: protein motif:HMM:PF13307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73914.1"
                     /translation="MRQTESGDISENTAFSTTGGPGRSLWSRLSTEGQPCSPTACQFY
                     NDCFLTRIRRASQRAHVVVINHSLLFSDFIAEGAVLGEYADLIIDEAHNFEKVATQYL
                     GAELSWWSVRDLLSALWQKEAHESGLLARVNGGLTAGTLASNRVKTFQIQAARAIDAV
                     SSTANAAQAFFERLNDTLRKPDRSSRFSHKQRYTSEDLPFGDAMAECEEFLSALEAIV
                     SETNTLVEWLREVGLGEVLQRDEFVLDLENRIADVVELTNIVADITGAERSDFVYWYE
                     LPADDTRLSVRLAAAPLNVGDQLRDSFFPRLRTCIMTSATLAVAGKFTYFLNRTGLTD
                     TVGERVRTLAVGSPFDYERQAFVAVPEWFPNPKSADFQDAVTDLVRDVVIRTRRGALV
                     LFTSYRMLNDTYRAVRQDFSSHGILLLSQGNDVSRTNLADTFRRDRESVLFGTESFWQ
                     GVDVPGDSLELLIIVRLPFAVPTEPLVIAQGEALRAAGKDPFLYMTVPEAAIRFRQGF
                     GRLIRSRTDRGAVIILDNRVITERFGRAFLGSLPTGHRTYESATELADALQQWFDRQS
                     D"
BASE COUNT          351 a          522 c          469 g          375 t
ORIGIN      
        1 cgctggcgac atggatgcga cagaccgagt cgggagacat ttccgagaac accgcgtttt
       61 caacgactgg cggacccggg cgcagtcttt ggtcgcgtct ctccactgaa ggacaaccgt
      121 gctcaccgac ggcgtgtcag ttctataacg actgctttct cacccgaatc cgccgcgcct
      181 cgcaacgggc tcatgttgtt gtgattaatc actcgcttct ttttagcgac ttcatcgctg
      241 aaggcgctgt acttggcgaa tacgccgatc tcatcatcga tgaggcgcac aacttcgaga
      301 aggtcgcgac acagtatctc ggtgccgaac tctcctggtg gtccgttcgc gatctccttt
      361 ccgcactatg gcagaaagaa gcgcatgaat cgggcttact ggctcgcgtg aatggcggtc
      421 tgacagcggg gacactcgca agcaaccgag tcaaaacctt tcagattcag gccgcccgcg
      481 ccatcgatgc cgtttcttcc acggcgaacg ccgcgcaagc gttttttgag cgactgaacg
      541 acacactgcg aaaacctgat cgatccagtc ggttctcgca taagcaacgg tacacctcgg
      601 aagatcttcc atttggcgac gcgatggccg agtgcgaaga gtttctgtca gcactggaag
      661 caatcgtaag cgaaacaaat accctcgtcg aatggcttcg tgaggtcggg ctcggcgaag
      721 tgctccaacg tgacgagttc gtgctggatt tggaaaaccg catcgctgac gtcgtcgaat
      781 tgacgaacat cgttgccgat atcaccggag ccgagcgatc cgatttcgtg tattggtacg
      841 aattaccggc cgacgatacg cgtctttccg tgcggctcgc tgccgcgccg ttgaatgtcg
      901 gcgatcaact ccgtgattcg tttttccctc ggttgcgcac gtgtatcatg acatccgcca
      961 cactggctgt tgccgggaag ttcacctact tcctgaaccg aaccggattg acagacaccg
     1021 tcggcgaacg agtccgtacg cttgcagtcg gctcaccatt tgattacgaa cgacaggcgt
     1081 tcgtcgccgt ccccgaatgg tttcccaacc cgaagagcgc cgattttcag gacgcggtaa
     1141 ccgacctcgt ccgcgatgtc gtcatacgga cgcgacgtgg tgcgctcgtc ttgttcacca
     1201 gctaccggat gctcaacgat acgtatcggg cggtacggca ggacttttcg agtcacggga
     1261 tcctgcttct cagccaaggg aacgatgtat cgcgcacaaa cctcgcggac acattccgtc
     1321 gtgaccgcga aagcgtactt ttcggtacgg aaagcttctg gcaaggcgtc gacgtgccgg
     1381 gagactcgct cgagcttctg atcatcgtgc gtcttccctt tgccgtgccc accgaacctc
     1441 tggttatcgc gcaaggtgaa gcccttcgcg cagcgggaaa agatccgttc ctttacatga
     1501 ccgtacccga ggcagcaatc cggtttcgcc aagggttcgg gcgcctaatt cgttcccgaa
     1561 ccgaccgcgg agccgtgatc attctcgata accgcgtcat caccgagcgg ttcggccgcg
     1621 cgtttctcgg ctcgctccca acgggtcacc gcacatacga gtctgcaact gaactcgccg
     1681 acgcgctcca acagtggttc gatcgccaat cagacga
//
LOCUS       DSCC01000011            2140 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1035102, whole genome shotgun sequence.
ACCESSION   DSCC01000011 DSCC01000000
VERSION     DSCC01000011.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1035102"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            273..734
                     /locus_tag="ENN56_00090"
     CDS             273..734
                     /locus_tag="ENN56_00090"
                     /inference="COORDINATES: protein motif:HMM:PF00011.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDS73915.1"
                     /translation="MSDTKLAPVAIHFADSDDAEAEMVRLLQQFMGTKKPFHGFTERA
                     WRPPTDIYETDCAFIVLIEIAGVEKGAISIEFANNAMRVSGVRRHHPESAHISYLQME
                     VKYGAYAIELRLPEGLNTDETNARYIDGFLTITIPKRPPQPSRSISIQGEG"
     gene            731..>2140
                     /locus_tag="ENN56_00095"
     CDS             731..>2140
                     /locus_tag="ENN56_00095"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00004.27,HMM:PF02190.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDS73916.1"
                     /translation="MSRMTVISDTPNEKRLHIGDQHEIPETLGLLVRHDLIMFPLMID
                     QLQIDDQAVKNLIDAALAGDRLIGIIGVPRSKEESVAGVEFPHVGTAAMIHKMLKLPD
                     GTVTILVQGVARVRFTEVVETEPFPSARVERLDTVLDDSVETKALAHNVASEFQRLIS
                     LVPQFPDDWKAVVQTMLARPDHLSDFVASNLNIEIEEKQELLETLSVSERLTKLTKRL
                     NSELEVVELANKIQNQVQKEMNRNQREFYLRKQLETIRHELGEEDQHATETRELRERI
                     EKANLPQHAREAAEREVERLSRMPTAAAEYSVSRTYVDWILSLPWSTTTEDNLEIRRA
                     QNVLEADHYGLSMVKDRILEYLAVRKLKPDMKGPILCFVGPPGVGKTSLGQSIAHALG
                     RNFVRMSLGGIHDEAEIRGHRRTYIGALPGRIIQGLRRAGSRNPLFMLDEVDKVGQDF
                     RGDPSSALLEVLDPEQNVAF"
BASE COUNT          504 a          613 c          623 g          400 t
ORIGIN      
        1 accgcggtag atacagtcgc aacgcctgat gtcgacgctc tccccggcga agaagccgaa
       61 gccgtcgatc gcccggtcga cgagccgata gtcaccgaaa ccgaagctga accagccgaa
      121 tcggctccgt ttgaagcatc ggagccggag caacctgaac cggtggagaa cgagactgct
      181 gccgagacga cggcgcaaga agaggccgtc gctgaggaag ctccgtcatc cgaggttgaa
      241 ccggtcaatc cctgatcacg cgagtgaatc ccatgtcaga tacgaaactt gctcccgtcg
      301 cgatccattt cgcggattca gacgatgccg aagcggaaat ggtgcgcctg ctccaacaat
      361 tcatgggcac gaagaagccg ttccatggct ttaccgaacg agcatggcga cctccgacgg
      421 atatctatga gaccgattgc gcgtttattg tgctcattga aatcgccgga gtggagaaag
      481 gcgcgattag tatcgagttt gcgaacaatg cgatgcgcgt ctccggtgtc cgacgccatc
      541 atccggaatc ggcgcacatc agctacctcc agatggaggt caagtacggc gcgtatgcca
      601 ttgaactgcg attgccggag ggattgaata ccgacgagac gaacgcacga tacatcgacg
      661 ggtttctcac gatcaccatt ccgaagcgac cgccgcagcc atcccgatcg atcagcatcc
      721 aaggagaagg atgagccgta tgaccgtcat ttccgataca ccgaatgaaa aacgcctaca
      781 cataggtgat caacacgaaa tccccgaaac actcgggctt ttggtgcggc acgatctgat
      841 catgtttccg ctcatgatcg atcagctcca gatagacgat caggcggtga aaaaccttat
      901 cgatgccgca ctcgcgggtg atcgcctgat cggtatcatc ggtgttccgc gatcgaagga
      961 agaatccgtc gccggcgttg agttcccgca cgttggcacc gccgcgatga ttcacaagat
     1021 gctcaagctc cctgacggca cggtgacgat tctcgtgcag ggcgttgcac gtgtgagatt
     1081 caccgaggtc gtggagaccg agccgtttcc gtcggcgcgg gttgaacgcc tcgacaccgt
     1141 attggacgac agtgtcgaga cgaaggctct tgcgcacaac gtcgcttcgg agttccagcg
     1201 actcatctca ctcgtgccgc agtttccgga cgactggaaa gccgttgtcc agacgatgct
     1261 tgcgcgcccc gaccacttga gtgatttcgt tgcgtcgaac ctgaatatcg aaatcgaaga
     1321 gaaacaggag cttctcgaga cgctctccgt gtcggagcgg ttgacgaagc tgacgaaacg
     1381 gttgaacagc gaactcgagg ttgtcgaact cgcgaacaag attcagaatc aggtgcagaa
     1441 ggagatgaat cgcaatcaac gcgagttcta cctccgcaag cagcttgaga cgattcggca
     1501 cgaacttggc gaagaagacc agcacgcgac cgaaacgcgt gaactgcgcg aacgaatcga
     1561 gaaggcgaac ctcccgcagc acgcccgcga agcggcggaa cgtgaagtcg agcgcctcag
     1621 tcggatgccg accgccgccg ctgagtactc ggtctcgcgc acatacgtcg attggatcct
     1681 cagtctgcca tggagcacga cgactgagga caacctcgag atccgacgcg cgcagaacgt
     1741 actcgaagcg gatcattacg gcctttcgat ggtcaaggac cgcattcttg agtatctcgc
     1801 ggtgcgcaag cttaagcccg acatgaaagg accgattctc tgtttcgttg gtccgccggg
     1861 agttggcaaa acgtcgcttg gacagtcgat cgcacatgct cttggccgga atttcgtgcg
     1921 tatgagcctt ggcggcattc acgacgaggc tgagatccga ggacaccgcc ggacctacat
     1981 cggagcgctc cctggacgaa tcatccaagg actacgtcgc gcggggagtc gaaatccgct
     2041 gttcatgctc gacgaggtcg acaaggtcgg acaggatttc cgcggcgatc cgtcgagcgc
     2101 gctcctcgaa gtgctcgatc cggagcagaa cgttgcgttc
//
LOCUS       DSCC01000012            1923 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040637, whole genome shotgun sequence.
ACCESSION   DSCC01000012 DSCC01000000
VERSION     DSCC01000012.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040637"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..>1923
                     /locus_tag="ENN56_00100"
     CDS             <1..>1923
                     /locus_tag="ENN56_00100"
                     /inference="COORDINATES: protein motif:HMM:PF07715.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS73917.1"
                     /translation="TQDVPMLLQTMPSAYAYSDAGNGVGYSYLKIRGFDQKRINVLIN
                     GIPHNGPTSHEVYWIDMPDLLANAEDVQVQRGVGNSLFGSNALGGTVNIITNTFNTEP
                     SISAYAGSGSFATRKYSFALNSGLVANRYVMYGRYSRITSDGYRDQSWTQLSSYFFGV
                     ARYDENMTTKVHIYGGPEKTHLSYYGVTRDYLEGNISGDRRVDRRYNPLTYENELDTF
                     TQPHYELHHDWQVTPKVRLSNTLYTIKGEGEYDQFRRDRNLTEFRLPTFSVADPNLYP
                     ASWYRRGNDGNPVADESDRYTITKSDLIRNRWVQNDEYGWLPRVTLDHKKGSLTVGGE
                     LRLHEGLHRGTITWLGATPPDVAPGHEYYRYTENKQTVSAFIHESYRLTPFVTVMGDV
                     QAVSHTYRLGDDPITNYEHETTYRWLSPKLGININLTDRIHTFATIARAQREPIASDL
                     VDPQDYWNIPNFTQTDTLRNGNAITAIRLSNPTVEPERMVNVEIGTGYRSPSLSVSAN
                     VYHMDFRNEIVPWVGQISEESGLPATGNADRSVHQGIEIEFGARPTSFLELSGNLNVN
                     ADRFIDYTEHAMDWDAWQTVSVVRDGNRIALFPTMLGNVRGLVTYQGASAGVAWRYVG
                     RQYLDNSEMTEYSIDPY"
BASE COUNT          475 a          547 c          522 g          379 t
ORIGIN      
        1 acgcaggatg tcccgatgct gttgcagaca atgccgtcag cgtatgcata ctccgacgcg
       61 ggaaatggcg ttgggtattc gtatctcaag attcgcggat tcgatcaaaa gcggatcaac
      121 gtcctcatca acggcattcc ccacaacggc ccgacctcgc atgaagttta ctggatcgac
      181 atgcccgatc tgctcgcaaa cgccgaagac gttcaggtac agcgtggcgt gggtaactca
      241 ctcttcggat cgaatgcact cggcggaacg gtaaacatta tcacgaacac cttcaacacc
      301 gaaccgagca tcagcgcgta tgcgggatct ggcagcttcg cgactcggaa gtacagtttc
      361 gcgttgaaca gcggactcgt cgcgaatcgt tacgtcatgt acgggcggta ctcacggata
      421 acatcagacg gatatcgcga tcaatcgtgg acgcaacttt catcgtactt cttcggtgta
      481 gcgcgctacg acgagaacat gaccacgaag gttcatatct acggcggccc ggaaaaaacg
      541 cacctgtcct actacggcgt cacgcgagac tacctcgaag ggaacatcag tggcgaccgg
      601 cgtgtcgacc gccgatacaa cccacttacc tacgagaacg agttggacac gttcacacag
      661 ccgcactacg aactgcatca cgattggcag gtcacgccaa aggtgcgtct cagcaatacg
      721 ctctatacca taaaaggcga aggcgaatac gaccaattcc gacgtgatcg taatttgacc
      781 gagtttcgcc taccgacatt cagtgtcgcc gatccgaacc tctacccggc gtcgtggtat
      841 cgccgcggga acgacggtaa ccccgtcgcc gacgaatctg atcgttacac aatcacgaag
      901 agcgacctca tccggaatcg gtgggtgcag aatgacgaat atggctggtt accgcgcgtt
      961 acgttggatc ataaaaaagg atctctgact gtaggtggcg agctgcggct gcatgaaggc
     1021 cttcatcgcg gaacgatcac gtggctcgga gcgacgccgc cggacgtcgc tcccggacac
     1081 gaatactacc gatacacgga aaacaagcag accgtgtcgg cgtttattca cgaatcatac
     1141 cgacttacgc cgtttgtgac ggtcatgggt gacgtgcagg cggtttcgca tacgtatcga
     1201 ctcggcgacg accccatcac gaactacgag cacgaaacga cctatcgatg gctctcaccg
     1261 aagctgggta tcaatatcaa cctgaccgac cgaattcaca cgttcgcgac aatcgcgcgt
     1321 gcgcaacgcg aaccaatcgc ttcagacctc gtagacccgc aagactattg gaacatcccg
     1381 aatttcacgc agaccgatac gctacgaaac ggcaacgcga tcaccgcaat acggctttct
     1441 aatccgacgg tcgaacccga gcgcatggtc aacgtggaga tcggtaccgg gtaccgttcg
     1501 ccgtcgctct ccgtaagcgc gaacgtctat cacatggatt tccgcaatga aatcgtcccg
     1561 tgggttggcc agattagcga agagagcggg cttccggcga cgggtaatgc agaccgatcg
     1621 gtacatcaag ggatcgaaat cgagttcggt gctcgtccga cttcattcct cgagctgagt
     1681 gggaatctca acgtaaacgc cgaccggttc atcgattaca ccgagcacgc catggactgg
     1741 gatgcgtggc agactgtgtc ggtggttcgc gacgggaatc gaatcgccct gtttccgacg
     1801 atgctcggaa acgtgcgcgg actcgtcacg taccaaggtg caagtgcagg cgtggcatgg
     1861 cgatatgtgg gacgtcagta tttggacaat tccgagatga cggaatactc gattgatcca
     1921 tac
//
LOCUS       DSCC01000013            2025 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037856, whole genome shotgun sequence.
ACCESSION   DSCC01000013 DSCC01000000
VERSION     DSCC01000013.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037856"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            147..863
                     /gene="tmk"
                     /locus_tag="ENN56_00105"
     CDS             147..863
                     /gene="tmk"
                     /locus_tag="ENN56_00105"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDS73918.1"
                     /translation="MGNGLPYVDLGDKTGHLIVVEGSDGVGRTTQLTLLRSWLEVQGY
                     GVIETGWTRSQLMSKTIDLAKEGNAVNALTFNLLYATDYADRLENEIIPALRSGFIVL
                     ADRYVYTAFARAIVRGADPEWIRQLYGFALNPDLVLYLHVDVDTLIRRVLLADSLDHW
                     EAGMDHNTGLDPYDSFMKYQGRVIREYDRMAEEYNFTTIDATRTVEEIQVDLREAISK
                     EIDIPHDLGLDKVEIGIGTV"
     gene            925..1497
                     /locus_tag="ENN56_00110"
     CDS             925..1497
                     /locus_tag="ENN56_00110"
                     /inference="COORDINATES: protein motif:HMM:PF14520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73919.1"
                     /translation="MTDELTSGGGYRYITDLPGIGPTKAAWIAEAGMDSVDAVRRAPI
                     DELAHVRGVGYVLARRIKDVLATSEIVLEPGADDELSAAQRVWRERTAALRVDLGARI
                     DALATNAKRLGLKPKFVQELQRLRSTLDRIPIERPPEDEEQRRKIAKHEQALLTLVES
                     AEEIDPRSGNYQKTLRKRLKSRRKKLNKWN"
BASE COUNT          454 a          558 c          600 g          413 t
ORIGIN      
        1 ccgatacagg cgcagcagaa gcaagtacgt gccgtcgtcg agcgcgaatt gcacgagtac
       61 ctcgcgccgt atctcgcgaa aaacaccgcc atcgcggcca ccgcaagcgc tcgctgaggg
      121 atagaggaat ggctacccgg caatacatgg gaaacgggct tccctacgtc gatttaggtg
      181 ataaaaccgg tcatctcatc gtcgttgaag gctccgacgg cgtcggacgc accacgcaat
      241 tgacgcttct acgctcatgg cttgaagtac agggatacgg ggtcatcgaa actggttgga
      301 cccgctcgca gctcatgagt aaaacgatcg atctggcgaa ggaaggcaat gcagtcaatg
      361 cgttgacgtt caacctgttg tatgcaacgg actatgccga tcgccttgaa aacgagatca
      421 ttcccgcgct gcggagcggc ttcattgtgt tggccgaccg ctacgtctat acagcattcg
      481 ctcgggcgat tgttcgtggc gcagatcccg aatggattcg gcagctgtac ggcttcgcgt
      541 tgaatccgga tcttgtcttg tatctgcatg ttgatgtcga cacgctgatt cgccgtgtac
      601 tgctcgcgga cagtctcgac cactgggaag caggtatgga ccacaacacc gggctcgatc
      661 cgtacgatag ttttatgaag taccaaggcc gcgttatccg cgaatacgat cgcatggccg
      721 aggagtataa cttcacaacc atcgacgcga cgcgtaccgt tgaagagata caagtagacc
      781 tgcgtgaggc gatttcgaaa gagatcgaca tcccgcacga cctgggattg gataaggtcg
      841 agatcggaat aggtacggtc taaggcattc aagttcgtgc gaggtgcaag gggatttcgg
      901 ggatatcgta cgggggagga cgtgatgacc gacgaactga caagtggcgg tgggtaccgg
      961 tacattaccg atctacccgg tatcggaccg acgaaggcgg catggatcgc ggaagccggc
     1021 atggattcgg tcgatgcggt gcgtcgtgcg ccgatagacg aactggctca cgttcgcgga
     1081 gttgggtatg tcctcgctcg tcggataaaa gacgttctcg caacctcgga aatcgttctc
     1141 gaaccgggcg cagacgacga gctaagtgct gcgcaacgtg tatggcggga acgtaccgca
     1201 gcacttcgcg tggatcttgg ggcacggatc gatgcccttg cgacgaacgc gaagcgtctc
     1261 ggactcaagc cgaagtttgt tcaggagctg caacgccttc gttcgacgct tgaccggatc
     1321 ccgatagaaa ggccgccgga agacgaagaa cagcgccgca agatcgccaa gcacgagcaa
     1381 gcgctgctga cgcttgtcga aagcgccgag gaaatcgacc cgcgcagtgg gaactaccag
     1441 aagacactgc gaaaacggct caagtcgagg cggaagaaac tcaacaagtg gaactgatat
     1501 ggtttcaaga cacggcgagg gcgattcgac tcaaggtcgg gtcgccctcg cgccgttaac
     1561 ttttctgggt gattacggcg tcactcgcag aatcgctgca ttgtcaccta ctgcgcgaag
     1621 cactccggcg tcataggtaa ccccacgaaa cactcgacct tcctgtcgaa gggcttcttg
     1681 ccacgttttg ccgccgtcat ccgtcgtaaa cacatactct tccgctgcga tcgcgccacg
     1741 attggcatcg aagaacgcca cagatagcgg cggcacaggt agctccgttt cgtgccattg
     1801 tgtactcgca ttgtgcttca cgagcacgac gtttttcggc gcgtcgttga tgagggtgcc
     1861 gataacaacg aagcggtcgt ctcccggcgt cgcgagtccg accgcttgca ggcttcgagc
     1921 ggtgcgtgtc gcgtcccatt tttcgccgtc tcgactccac cagatacgtc cttctctatc
     1981 gagcgcgcat acggtgccgt tcggtgtgat tgtcacatcc acaag
//
LOCUS       DSCC01000014            2500 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028489, whole genome shotgun sequence.
ACCESSION   DSCC01000014 DSCC01000000
VERSION     DSCC01000014.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028489"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
BASE COUNT          506 a          704 c          792 g          498 t
ORIGIN      
        1 ccgccactcg acgacgagcg cgcggtaagt gtagcacggc aggctctcat cgacgcggcg
       61 gctaaagccg gctgggagtt ggtcggtgat gacgaatggc cggcggagcg acagattgtc
      121 gtgaagcttg gcgacggcgt ccaaaccgag ccgttcaagc cggcggtgtt cccctcggtc
      181 gacgagcagt cgtattcgat ccgatcgcag aatgatggcg accgcagtgt tgttacggta
      241 gaagcaggtg gtgcgctcgg tgcgacgttc gcgctgtctc gaatcgccga ttcgatacgt
      301 tccgcgtgtg cgtggcctcc ggcggatgtc gatgaacgtc cccgtttcgc gcagcgaatg
      361 tcattcctct ttatgtcgta tccaaactcc gacgaagcgc cgtatgttga catggacgcc
      421 gcattggtgc aactcgatcg tgcgatcgcg cagctcgacg agacgctact tgccggcggc
      481 acgttcgtgc aggtcctcgg aagccgtagt ctcttcaagt ggggtgatga gaagcttgac
      541 gaacgggctg acgcgtgtcg tgaagtcttc ggcgaactta tcgtcgccgc gcacgaacgg
      601 cggctactcg tttacagcat ggacgacgaa ttcctctacg agccggcatg gtttgagagc
      661 accggtgcta cgttcagcac cgacgatgcc cgcttctggg atgcgctcac gtcgaaatat
      721 cgcgacctcc tcgaagcgtt gccacacttg gatggtatcg gcacgcgcac cggtgaagtg
      781 acgccgcaag gccggatact cgcgtgggac ctgatccata caccggacga tcgctcgatc
      841 gaaggcaact accggcgttt cctcagagca atgcacggtg tcgtcgtcgg tgagttcggc
      901 aggcagtacc tccaccggac gtggacagtc aacacgtggg agcagtcgag cgtacccgag
      961 atcttccgcc ggacgttcac cgaagacgtt ccgatggaga acttcatcgc gagcatcaag
     1021 acgacaacgg gcgaccagtg ggagtggcag ccgatcaacc cgaccttcgg tcagacacct
     1081 catacgactg ccgcacaggt cgaaacctca cgcgcacagg actacttctc cggtcctccg
     1141 gatttttctg ctgagttctc gcaagcggga cttgaatacg cattcgagca cggcgcaacg
     1201 gtggcgggag tgaatctccg cgaccgctgg cagaagaccc tctgggcggc gctcgattac
     1261 gtcacatggc gcctcggatg gaacccgtat cagtcggtgc gaaagctcac cgccgagtgg
     1321 gccgctgccc agattgatcc gaaagtcgcc gatcgcgtag cagagatgct gctcgacttc
     1381 gacgatatct accgcgacgg gttccacatc cgtggacccg cgtatcacac gtgggaaccg
     1441 cttgtgcatg ttcgcaccgg cttcgtgtgc atggggaatc cgcttcttga caacggacgc
     1501 ggccagtatc gattcctccg cgatctctat ctgcaagcga aaccagaact cgaaagcgga
     1561 ctgcagatca tggatgcggc cacgaagcga ttcgatgtgt ggtatgacga ttaccgccga
     1621 tggctgcatg aactcgacga gccgaaacgc ggtgattggc tgatcccgat cttgcaatgg
     1681 ggaagcgacg tgttgcgtct gaactacgcg tacgtcaacg cgttcttgcg gttcttcgat
     1741 ttcgccgacg ccgcgcgtgc cggtggcgat acacggtacc tcgatcgtgc tgctacctcg
     1801 atcggagagc tggaggcaga gttagagcgc ttccgcgcga accgtgcaga gtcgaactac
     1861 cgcccggcgt tctcggcagc ggaaaacgta cagggcattg atgtcttcct cacatttgcg
     1921 aagccgggtc tcgatgacct tgccggggtt atctcgatga tgcgcagtgc tccggacgaa
     1981 gctggcgtgc gtcggatgta tgaagaagcg cgggaacgcg atacggcgct tctcgccggg
     2041 aatgatggcg cgcagattct cgtgcagtgg aaagggcgcg tcgattcgcg tgatgtattc
     2101 cggtttggct tgaaggacgc aacactggtg atcgagcatt acctcggcga cggcggcgtg
     2161 acggattcgt ggaccggcaa tccgaagccg gaaggtagcg gaagggttgc gttgcgggtc
     2221 gaccgcggag cagaacgcgg ctgggcgtgg gttcttgaag agccgagcga ggataacaac
     2281 gaaacgctcg cgatacagat tgacgacaag aagcccgggt acgggtccta cgaagtaaca
     2341 gcgttctgga tacccgaata atcggagttt ttgttagaac cggcttatga gtgtttgacg
     2401 ccgggtaccg ttatcggtac ccggcgcgtt ttcgataacg atcggtcggg aaccgactac
     2461 gcagttcaac cgtcccaccg cctgtatgga ttcgcagaga
//
LOCUS       DSCC01000015            2481 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028762, whole genome shotgun sequence.
ACCESSION   DSCC01000015 DSCC01000000
VERSION     DSCC01000015.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028762"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..668)
                     /locus_tag="ENN56_00115"
     CDS             complement(<1..668)
                     /locus_tag="ENN56_00115"
                     /inference="COORDINATES: protein motif:HMM:PF14903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HDS73920.1"
                     /translation="MRRGYPLYFIVRRHIRADTTLPRSSRMIPIELRTGDLRMARWIL
                     VFSAVIALAACSGDKGTTEGEVRGGLYPMATMAGYGFIDSTGRVVIEPQFMQVTDEWV
                     DGLMSVSIGELYGLVDTSGRIVLPPTQIRQVFPEISFRNGLARAETDTGIGFINRRGD
                     FVIPARFSNTYGFNEGVAPVRDMEKRWGYIDTTGAWVIEPQFDDVTPFTDGLAVFQKD
                     RKWG"
     gene            complement(669..1736)
                     /locus_tag="ENN56_00120"
     CDS             complement(669..1736)
                     /locus_tag="ENN56_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73921.1"
                     /translation="MTPRQRVETALRGDLPDVIPFTIYANKLPRCQVELELRNAGACI
                     LERSPGALSVETPNVKTTTVQTQENGAAIVRTAIETPAGTLTTVNRPAPGTTWHLKRM
                     FSGPDDYAAIIALIRDRQWRENYKPFVEARKRYGEGSFLRANFGYSPLQEILYHIMGV
                     EQFSIEWAERRDDLMKLYDVLTEDRRKEYQLMADSPVMAVNYGGNVSPEIMGIDRFRQ
                     YILPHYEEAAEILHRTGKLIGVHFDANNLALADAIAESSLDYIEAFTPPPDCDMSVAE
                     ARRRWKNKVLWINFPSSVHHGGVPAVEELMREILHEAAPGDGFLVGITEDVPDHAWPE
                     TFPAIARVINDEGRTPMPLAC"
     gene            2049..>2481
                     /locus_tag="ENN56_00125"
     CDS             2049..>2481
                     /locus_tag="ENN56_00125"
                     /inference="COORDINATES: protein motif:HMM:PF06560.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73922.1"
                     /translation="MSQSALLDAAAALSTFLDVQSGRLAVCSNTQRNRLSDLTGVFSD
                     EETRAALVNNANPVIYEVYRTEPAEKQDTALCYSSTIVHSGRVGSEYYMTRGHSHPSL
                     DAPEVYLTIRGEGMMLLQSDDFHVQALAMGPGTVLYVPGSII"
BASE COUNT          527 a          736 c          679 g          539 t
ORIGIN      
        1 atgccccact tccggtcttt ctgaaatacc gcgagtccgt cggtgaacgg cgtcacgtca
       61 tcaaattgcg gctcgataac ccatgcgccg gtcgtatcga tatagcccca gcgtttttcc
      121 atgtcgcgaa ccggagcgac tccctcattg aacccataag tattcgagaa tcttgccggt
      181 atgacgaaat caccgcgacg attgatgaaa ccaattcccg tatcggtctc cgcacgcgcg
      241 agaccgtttc ggaacgagat ttcggggaat acctgacgaa tctgcgtcgg cggcaatacg
      301 attcgcccgg acgtgtcaac cagcccgtag agttcgccaa tactcacact cataagcccg
      361 tcgacccact cgtcggttac ctgcatgaac tgcggttcga tgacgaccct acccgtagaa
      421 tcgatgaaac catatcctgc catggtggcc attgggtaga ggccgccgcg aacttctccc
      481 tcggtcgtgc ctttgtcgcc gctacatgcg gcaagagcga tgacggcgga gaaaacgaga
      541 atccatcgag ccatgcgtaa gtctcccgtg cgcaattcaa tcggtatcat tcgcgaacta
      601 cgcggcaacg tagtatccgc tcgtatgtga cgtcgaacaa tgaaatatag aggataaccg
      661 cggcgcattc aacatgcgag cggcatcggc gtgcgtccct cgtcattgat cacacgcgca
      721 atcgccggga aggtttccgg ccacgcatga tctggaacgt cttcggttat cccgacgagg
      781 aacccatcgc ccggtgctgc ttcgtgaagt atttcccgca tcaactcctc caccgcagga
      841 acaccgccgt gatgtaccga cgaaggaaag ttgatccaga gcaccttatt cttccatcga
      901 cgacgcgctt cagcgacgga catgtcacaa tccggcggtg gcgtgaacgc ctcgatatag
      961 tcgagcgacg attcggcgat ggcgtcggcg agcgcgagat tgttcgcatc gaagtgcaca
     1021 ccgatgagct ttcccgtgcg gtgaaggatc tcggcggctt cctcatagtg cggcaggatg
     1081 tattgacgaa aacggtcgat gcccataatc tcgggactga cgttgcctcc gtagttgacc
     1141 gccatcaccg gagaatccgc catgagctga tactccttcc gccggtcctc ggtcaggacg
     1201 tcgtagagct tcatgagatc gtcgcggcgc tccgcccact cgatactgaa ctgctcgacg
     1261 cccatgatgt ggtagaggat ctcctgcaac ggtgaatacc cgaagttcgc tcgcagaaat
     1321 gatccctcgc cgtaccgctt tcgagcttcg acgaatggtt tgtagttttc gcgccactgc
     1381 cggtcgcgga tcagggcgat gatcgccgcg tagtcgtcgg gaccgctgaa catccgcttg
     1441 agatgccacg tcgtgcccgg cgccggtcga ttcaccgtcg tgagcgtccc cgccggtgtc
     1501 tcaatcgccg tgcggacgat ggctgctcca ttctcctgcg tctgaaccgt cgttgttttg
     1561 acattcggcg tttcgacgga aagcgctccc gggctccgtt cgaggatgca cgcgccggcg
     1621 tttcgcagtt cgagttcgac ttggcaacgc ggaagcttat tcgcgtaaat cgtaaacggg
     1681 atcacatcgg gaagatcgcc gcgaagtgcg gtctcgactc gttgccgggg tgtcattcga
     1741 gttactcctc aaccaaagtc ggggacggaa acagtacaca caccgacgaa tcatactgcg
     1801 atacgtgaca ccgaccaacg atgctcgatt gacgaatcac tgaccgcgcc aattcacaac
     1861 gatgtcggga agccccgtgg gatgggtaat tgcccacacg accgttgttt caatcgtcgt
     1921 ttcacgtttt cttcttactc tgtccgttga tgcgacatgg tgggagcgta ttcacaccgt
     1981 ttcaatactc gcatgtgttc atgcaagacc ggttatggta aaacggttat tccttcggag
     2041 gtccgtgaat gagccagtcg gcgctactcg acgcggcggc ggcgttgtcg acattcctgg
     2101 atgtgcagag cggtcggctc gcggtctgtt cgaacacaca gcgcaatcgt ctatctgatt
     2161 tgaccggtgt attctccgac gaggaaaccc gcgctgcatt ggtaaacaat gcgaatcccg
     2221 tcatctacga ggtgtaccga accgaacccg cggaaaaaca ggataccgca ctttgttatt
     2281 cgtcgacgat cgtacattcc ggacgcgtcg gcagcgagta ctacatgact cgcggccact
     2341 cacatccgag cctcgatgcg ccggaagtct acctgacgat acgcggcgaa ggtatgatgc
     2401 tcctgcaatc ggacgacttc cacgtacagg cgctcgcaat gggacccggg accgttctct
     2461 acgtgccggg cagcatcata c
//
LOCUS       DSCC01000016            2437 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029469, whole genome shotgun sequence.
ACCESSION   DSCC01000016 DSCC01000000
VERSION     DSCC01000016.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029469"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            20..1084
                     /locus_tag="ENN56_00130"
     CDS             20..1084
                     /locus_tag="ENN56_00130"
                     /inference="COORDINATES: protein motif:HMM:PF00583.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS73923.1"
                     /translation="MFDEFPTLFSPENRECLRVISVDDEIVSAICYQIRPAIIYGVTI
                     AVASLGAVCTYESYRGHGFATQLLDDTFAAARKEGADVMLISGQRGLYQRAGCAIAGH
                     EYRFSLTRSEIDSISSTADATVTLATDTDTEVLADLQRTEPVRFVRSPEDWRAFATLF
                     RLVRPDMPEPFGVMRCWITRINDLPVAYVVLKAYRSKEGRVTMHLVECAGSRRHVLAG
                     IAQVAKELSVEVVEGSLLPGDVDGRSVLSGIGVTPTVHPLGGHRFSILRVDILDRYRN
                     WLVEKVGPAKARDLSITEESGTWSLRTDAKQIPVGDLEQMNSVLFGDAVHDLRGSDDA
                     LEVWRRALPLPWLKPGLNYI"
     gene            1126..2268
                     /locus_tag="ENN56_00135"
     CDS             1126..2268
                     /locus_tag="ENN56_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73924.1"
                     /translation="MQPHESLIVVLVRSRRDWHMVRTRHWYRLPVKNGPTDVSARWIA
                     FYLPKSFGENERGVRWYARIRNVEIVRRIDLFPNEPDHPRAQNHYFAISFDPPIRLTR
                     PLLPDRGRRFVWSRATLWRLTAAQTFDDLFREEPLAPPESDQVLVGVVPRESDLQIAR
                     LQRWYRIPTNMVRDWSTPSWIAFYFGRGFGGDAGNIRYYARVWHADIVRRLEMFPDQP
                     RHPRANAEYVRIHLSELHERPTPIRSRRQRQLVVLPTTWERFMMAADVNDLVVGDEVE
                     EAFYGRLSTAGYRPERAYHVRGTNAYRLTDYAIFCSERNIEIDIASQTLPKRMLVGVT
                     DANDAGVPDGWTALRLSRFDLTKRRDESIDRVRRLVDASGGILTES"
BASE COUNT          529 a          713 c          665 g          530 t
ORIGIN      
        1 tcccggcccg aatacatcga tgttcgacga attcccgacg ctgttctctc cggaaaacag
       61 agagtgtctt cgcgttattt ccgtcgatga cgaaatcgtc tccgcgatct gctatcaaat
      121 ccgaccagcg attatatacg gggtaacaat cgccgtcgca tcgctcggag ccgtctgcac
      181 gtacgaaagc tatcgcggtc atggtttcgc aacgcaattg ctcgacgaca ccttcgcggc
      241 agcccgcaag gaaggtgccg atgtcatgct catttccggc caacggggat tgtatcaacg
      301 agccgggtgc gccatcgccg gacacgagta tcggttctca ctgacccgct ccgagatcga
      361 ctcgatctct tccactgcgg acgccacagt aactctcgct accgacacag acaccgaagt
      421 actcgcggat cttcagcgca ccgagcccgt tcgattcgtg cggagccccg aagattggcg
      481 agcatttgcc acgctctttc gcctcgttcg cccggacatg cccgaaccgt tcggcgttat
      541 gcggtgttgg attactcgaa taaacgatct tccggtcgcc tatgtcgtat tgaaagccta
      601 tcgatcgaag gaaggtcgcg ttacgatgca tcttgtcgaa tgcgccggat ctcgccggca
      661 cgtacttgcc ggaatcgcac aagtagcaaa ggaactgtct gtcgaagtcg tcgaaggttc
      721 gcttcttccg ggcgacgtcg acggtcgatc agttttgtcg ggaatcggtg taacgccgac
      781 cgtacatccg ctcggcggac accgtttctc gattctgcgc gtcgatatcc tcgatcggta
      841 tcgaaactgg ctcgtcgaaa aagtcggtcc ggcgaaagct cgagatctat cgatcacgga
      901 agagtccggt acgtggtcgc tcagaaccga cgccaaacag attcccgtcg gagatctcga
      961 acagatgaac agcgtattgt tcggcgacgc tgttcacgac ctgcgcgggt ccgacgatgc
     1021 actcgaagtc tggcgcaggg cgcttccgtt accgtggctc aaaccgggtc tcaactacat
     1081 ctgagctatt cacgtcgacg accgcgcaaa actgcggagt agagtatgca gccgcacgaa
     1141 tcgctgatcg ttgttctcgt tcgatcacgt cgcgattggc acatggtacg cacacgacac
     1201 tggtaccgtt tgccggtaaa gaacggtcca acggatgtct ccgcgcgttg gatcgcgttc
     1261 taccttccca aatcgttcgg cgaaaatgag cgtggcgtgc ggtggtatgc acgcatacgt
     1321 aacgttgaaa tagttcggcg tatcgacctc tttcccaacg aacccgatca cccgcgcgcg
     1381 caaaatcact acttcgcgat ctcattcgat ccaccaatcc gacttactcg accacttctc
     1441 cccgaccgtg gacgtcggtt cgtatggtcg cgcgcgacgc tttggcgcct gaccgccgct
     1501 cagacattcg acgacctatt tcgcgaagag ccgcttgcac caccggagag cgatcaggtc
     1561 cttgtcggag tcgtaccacg ggaaagcgac ctgcaaatcg ccagactcca gcgatggtat
     1621 cggataccga caaacatggt acgcgactgg tcaacgcctt cgtggattgc gttttacttc
     1681 ggtcgtggct ttggcggtga tgcgggcaac atccgctact atgcccgcgt gtggcatgcc
     1741 gatattgttc gacgccttga aatgttcccg gatcaaccgc gtcacccacg tgcaaacgcg
     1801 gaatacgtcc gaatccatct cagtgagcta cacgagagac cgacacccat tcgtagtcgt
     1861 cgacaacgac agcttgtagt tttgccgaca acgtgggaac gcttcatgat ggccgcggat
     1921 gtaaacgacc ttgtggtggg tgatgaggtc gaagaggcgt tttacggaag actgagtacc
     1981 gctggatacc gtccggaacg cgcgtatcac gttcgcggaa ccaatgcgta tcgccttacc
     2041 gattacgcca tattctgttc ggaacgaaac atcgagattg acatcgcatc gcagacgcta
     2101 ccaaagcgta tgctcgtcgg cgtcaccgat gccaacgacg ccggcgttcc cgacgggtgg
     2161 accgcgctca ggctatcgcg gttcgacctt acgaaacgcc gagacgagag tatcgaccgt
     2221 gttcgccggc ttgttgacgc gagtggcggg attcttaccg aatcttgagg gaggcaaggc
     2281 cgatcatcgc aagaataccg gcaatgatcc agatacggat gacaaccttt gtctccgcga
     2341 gtccttggta ttccatctga tgatgaagcg gcgcccggta gaatatcctg cgaccaagga
     2401 agttctcacc gatcttctct tgaatgagag agctgaa
//
LOCUS       DSCC01000017            1976 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039126, whole genome shotgun sequence.
ACCESSION   DSCC01000017 DSCC01000000
VERSION     DSCC01000017.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039126"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            236..448
                     /gene="rpmB"
                     /locus_tag="ENN56_00140"
     CDS             236..448
                     /gene="rpmB"
                     /locus_tag="ENN56_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDS73925.1"
                     /translation="MSLACAICGKTSARGNNVSHANNRRKRTFKPNLQRIRASVNGQP
                     KYIRVCTDCIRSNKVEKAVRPSLLVD"
     gene            complement(538..>1976)
                     /locus_tag="ENN56_00145"
     CDS             complement(538..>1976)
                     /locus_tag="ENN56_00145"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:PRK08645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDS73926.1"
                     /translation="LETFTDVETLTAALIAVRKITSDMPVICEMAFSEYDRTGRATGD
                     EAVRVMRQLSAHGADVVGGNCGSGPTGLLPYIRRMAETSDMPISALPNASYPQYVDGR
                     YVFRSTPEYIAERAEELVEAGANIIGGCCGTGPEHIRLIAQRLNGRVPAIRRRVDRPS
                     PLVELYERIERRPTGFFDDVGDRSVILVEIDPPKGLDYAYVIEAAHALKRAGIDAMTS
                     ADNSLASIRMSTFVMSHLVQQETGLPVIVHCACRDRNLIGQQSELLGAAALGLRYILA
                     ITGDPASMANIGASSVYDTNSIGLIKLIHTLNQGQNLAGAPTQRPTDFVVGCALDPSG
                     HQMDGQLRRLAKKIEAGAQYVMTQPLYDADRIRELYDRTRAEFPDTPVFCGVMPLASK
                     RNAEFLHNEVPGIRIPDEIMERMRNAGDEASKEEGITIAAELIETALEAGAPGIYLIP
                     PFNKYEYALKLIPIIKAWDRKHRTATKR"
BASE COUNT          378 a          583 c          578 g          437 t
ORIGIN      
        1 aatcattatg atacgtccgc aatccgttcg gtgtcaacgt atcgacatgc tcgtcggttc
       61 ccacagtgag tattcgtagc actgtgctgt gtcttgacgc cgatcgattc atgcaatatc
      121 attaaaaact tgtcggtatg tatctatgaa tgatgatttg cgtcgagaac gcgcagggtg
      181 cgaggtttca cgcgccatga gagtcgcggg cggcgtttca cacgaggaat acgatatgtc
      241 acttgcttgc gcgatttgcg gaaagacatc ggcgagaggg aataatgtca gccacgcgaa
      301 taatcggcgg aaacgcacct tcaagccgaa cctgcagcgc atccgcgcct cggtgaacgg
      361 tcagccgaag tacatccgcg tgtgcaccga ctgcatccgg tcgaacaagg tcgaaaaggc
      421 ggttcgcccg agcctgcttg tcgactgatc tcgcccacac atcgaataca cgaacgtccc
      481 gtacggtgct ataccatcgt acgggacgtt ttgttgtacc tggttctcgc gaacgaacta
      541 ccgtttcgtc gccgtccggt gtttccgatc ccatgccttg atgatgggaa tgagcttgag
      601 tgcgtattca tatttattga acggcggaat gagatagatt cccggcgcgc cggcttcaag
      661 cgccgtttcg atcagttccg ccgcgatggt gattccttct tcctttgacg cctcgtcacc
      721 cgcattccgc attcgctcca tgatctcgtc gggaatacga attcccggta cctcgttgtg
      781 gagaaactcc gcgttccgtt tgctcgcgag cggcataact ccgcagaaga ccggtgtgtc
      841 ggggaattcc gcgcgtgttc ggtcgtagag ttcgcgaatg cggtcggcat cgtagagcgg
      901 ctgcgtcatg acgtactgcg cgcccgcttc gattttcttc gcgagtcgcc ggagttggcc
      961 gtccatttga tggccgctcg ggtcgagtgc gcaaccaacg acgaaatcgg tcggacgctg
     1021 tgtaggcgcg ccggcgaggt tctggccctg attgagcgtg tgaatgagct tgatcagtcc
     1081 gatcgagttc gtgtcgtata ccgatgacgc accgatattc gccatactcg ccggatcacc
     1141 ggtgatcgca aggatgtagc gcaagccaag cgccgccgcc cccaggagtt ccgactgctg
     1201 cccgatgagg ttccggtcgc gacacgcgca gtggacaatc accggtagac ccgtctcctg
     1261 ctggacgaga tggctcatga cgaatgtgga catacggatg gacgcgagcg agttgtctgc
     1321 tgacgtcatc gcgtcgatgc cggctcgttt cagtgcgtgc gccgcctcga tgacgtacgc
     1381 atagtcgagc cctttcggcg gatcgatctc tacgaggatc acactgcggt cgccgacatc
     1441 gtcgaagaat cccgtcggac gccgctcgat tcgctcgtac aactccacca gcgggctcgg
     1501 tcgatctaca cgcctgcgaa ttgcgggtac acgaccgttc agccgctggg cgataagccg
     1561 gatatgttcc ggtccggtcc cgcagcatcc accgatgata ttcgcgccgg cctcgacgag
     1621 ttcttcggcg cgctcggcga tgtattccgg cgtggaacgg aacacgtacc gaccatcgac
     1681 gtactgcgga tagcttgcat tcggcagtgc gctaatcggc atgtcgctcg tctcggccat
     1741 gcgtcggatg tacgggagca aaccggtcgg accgcttccg cagttaccgc cgacaacgtc
     1801 cgctccgtgc gcacttagct gccgcatgac gcgaaccgcc tcgtcacccg tggcacgtcc
     1861 ggtacgatcg tattcactga acgccatctc gcagatgacg ggcatgtccg acgtgatctt
     1921 tcgcacggcg atcagcgccg cggtaagcgt ttcgacgtcg gtgaacgtct cgagca
//
LOCUS       DSCC01000018            1990 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038750, whole genome shotgun sequence.
ACCESSION   DSCC01000018 DSCC01000000
VERSION     DSCC01000018.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038750"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            20..436
                     /locus_tag="ENN56_00150"
     CDS             20..436
                     /locus_tag="ENN56_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73927.1"
                     /translation="MNDYGGGAFLSADYALRDDLALLLNLGGMFFSGIDGEKNSKPWV
                     YQDWYVYTATIGAKYYPVKGTEQPPFIGAEVGYYRWDGNGSDSAGGGKNKISFTPFIG
                     AETSVGSVGINMKLGYAMMADFQLIQFGVGFQLLNF"
     gene            complement(400..1884)
                     /locus_tag="ENN56_00155"
     CDS             complement(400..1884)
                     /locus_tag="ENN56_00155"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73928.1"
                     /translation="MRKPERPNILMIMADQLRHDWLGCAGADHVSTPNINGIAERGVR
                     FTQCTTPSPICAPARISLASGLMPHRLGSVDNSAYLPISIPTYYQRLRDYGYHVGCVG
                     KLDLAKSDRYNGMSGARPRTFGWGFTHPVEAEGKMHAGNSATPIGPYTKWLDDRGLLQ
                     KFHEDYSVTRPKLPWFEAQTLDSALPTEAFEDVWIGRRAEQWLEEIDGDFPWHLLVSF
                     VGPHNPFDPPTEYADRYRGEAMPKPIADDLKDKPLWIQNRAAQNPGTDEQHAIARRQY
                     SAATTAIDDAVGGVLRALEARGWLETRTSLLPGITANFSATTNSTPNTQRTKHRCACR
                     LSSRDRTSTVGKRPTRLWNWRTSIRRCATLRECRRWRISTAAACLASCTIRHESTVER
                     PSRRRETIARFEPASGSTSRASTISRSCTISPPIRTRRRTSSRNARKLRKISGNDSTR
                     GWSKGNTCVADRDKKARDTHVPRAFLIYPYSVDQKLRSWNPTPN"
BASE COUNT          387 a          563 c          583 g          457 t
ORIGIN      
        1 caatgatgcg ctttggcgca tgaacgacta tggaggcggt gcgtttcttt ctgcggacta
       61 cgcgctcaga gatgacctcg ctctcctatt gaatctcggt ggtatgttct tcagtggcat
      121 cgacggcgaa aaaaatagca aaccatgggt gtatcaagac tggtacgtgt atacagccac
      181 gatcggcgcg aagtactatc ccgtgaaagg aacggaacag ccaccgttca tcggcgccga
      241 agtggggtat taccggtggg atggcaacgg ttctgacagc gcgggcggcg gaaagaacaa
      301 gatctcattt acaccgttta ttggcgcaga aacgagcgtt ggttcggtcg gcattaacat
      361 gaaattaggt tatgcgatga tggcggattt tcaactcatt cagtttggtg tcggattcca
      421 gcttctcaat ttctgatcaa ctgaatacgg ataaatgaga aaggcgcgcg gtacgtgtgt
      481 atcgcgcgcc tttttgtcgc gatcagcgac gcaagtgttc cccttcgacc aaccgcgcgt
      541 tgagtcgttg ccggagatct ttcgcaactt ccgggcgttt ctcgatgagg ttctccgtct
      601 cgttcggatc ggcggtgaga tcgtacagct ccgagagatc gttgaggctc tcgatgtact
      661 tccactcgcg ggttcgaatc gcgcgatagt ttccctccgt cgtgacggcc tctccacggt
      721 gctctcgtga cggatcgtgc acgacgccaa gcacgctgcg gccgtcgata ttctccagcg
      781 acggcactcc cgcaatgtcg cacaccgtcg gattgacgtc ctccagttcc acaagcgcgt
      841 cggacgtttt cccaccgtcg atgtccggtc ccgcgatgat aagcggcacg cgcatcgatg
      901 tttcgtacgc tgcgtgtttg gtgtagagtt ggtggtcgcc gagaagttcg ccgtgatccc
      961 cggtaacaac gatgtacgtg tttccaacca gccacgcgcc tcaagtgctc ggagaacgcc
     1021 accgaccgcg tcatcgatag ctgttgtcgc tgcgctgtac tgccgccgtg cgattgcatg
     1081 ctgctcgtcg gtgcccgggt tttgcgcggc gcggttctga atccagagag gtttgtcttt
     1141 gagatcatct gcaatcggct tcggcatggc ctctccgcga taccggtccg catactccgt
     1201 tggcggatcg aatggattgt gcggtccgac gaagctcacg aggagatgcc acgggaagtc
     1261 gccgtcgatc tcttcgagcc actgctcggc gcgtctgccg atccacacgt cctcaaacgc
     1321 ttcggtcggt agagcgctgt cgagcgtctg cgcctcaaac cacggcagtt tcggacgcgt
     1381 tacggagtag tcttcgtgga atttctgcag aagaccgcga tcgtcgagcc atttcgtata
     1441 cggaccgatc ggcgtcgcgc tatttcccgc gtgcattttc ccctcggctt cgacgggatg
     1501 ggtaaatccc cagccgaagg ttcgcggacg cgcgccgctc ataccgttgt atcggtccga
     1561 tttcgcaaga tcgagcttcc ccacgcaccc gacgtgatat ccgtaatcgc gtagtcgctg
     1621 gtaataggtc ggtatcgaga tcgggagata cgcgctgtta tccaccgagc cgagacggtg
     1681 cggcatgagc cccgacgcaa gcgaaatacg cgccggagcg cagatcggcg agggagtcgt
     1741 gcactgggtg aaccggactc cacgctcagc gattccgttg atattcggcg tactgacgtg
     1801 atccgcaccg gcgcaaccga gccagtcgtg acgaagctga tccgccataa tcatcaggat
     1861 gtttggtcgt tcgggctttc gcatacaaat cctccaaatc tccgagggcg attgtcttca
     1921 gaaagagtgc tcccgcgacg agtcggacgc aaggaaacgg cgtgtcgcag tgtctgttat
     1981 tcctccacag
//
LOCUS       DSCC01000019            2059 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037006, whole genome shotgun sequence.
ACCESSION   DSCC01000019 DSCC01000000
VERSION     DSCC01000019.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037006"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(277..1647)
                     /locus_tag="ENN56_00160"
     CDS             complement(277..1647)
                     /locus_tag="ENN56_00160"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02954.17,HMM:PF14532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS73929.1"
                     /translation="MPSPKVLLVDDERSVTTLLSLALRDAGYEADAANSGQEAVDRLS
                     EEVFDVVISDLRMDRVDGLDVLRAARHRYPAPAVLIMTGHGSIDSAVEAMKLGASDYL
                     TKPVDPNELKVVLERVLEHRQLLSELEVLRGKVEQLQLDDLIGTSKPMQHVFRTIQRI
                     ARTDVTVLITGESGTGKELVARSIHNRSSRADGLFLTLNCSALTDTLLESELFGHAKG
                     AFTGAITAKKGLFEDADGGTILLDEIGDMELGAQAALLRVLESGEIRRVGETRPIAAD
                     VRVLASTNRDLEKAIEEGTFREDLYYRLRVVPIRIAPLRERTEDILPLAQHFIVRYQE
                     KFSRQGFKLSPESVSLLTRYRWPGNVRELQHAIERAILLSDDETIKPEDLPPEVSNPA
                     PAETNTAPMTLDEIEKQHILRRLEQCEGNRSEAARQLGVSRNTLARKLKSYGVAGSGD
                     DEDEDE"
     gene            complement(1619..>2059)
                     /locus_tag="ENN56_00165"
     CDS             complement(1619..>2059)
                     /locus_tag="ENN56_00165"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDS73930.1"
                     /translation="VRIIEGDSDAVGNVVIETEWPNDLPNVSVDGDQIRQVLWNIILN
                     GLQAMNGSGTLTVRIQPESSEYVSLDITDTGGGIPQEELSRVFEPFHTTKPRGTGLGL
                     AIARRIIQGHDGSIQIKSRVGEGTTVSISLPTDNVNALTESTPR"
BASE COUNT          415 a          614 c          587 g          443 t
ORIGIN      
        1 gtagtagaga acgaacggga tcgcggcggc aacgatgtag attgagagca ggagaatcag
       61 cacacccgtt ccgacgacgg gatggacacg gtccgcaagt gcgacgatgt ccgcggtctc
      121 gaccacaacg acgacgacga acacggcgag aaggcccgcg ccgccgatca tcaggatacg
      181 tcgtattaat tggaacaaat ctgtacgcga gctattttgt tgtggtgtac cggatgtcga
      241 cgcggcgtcg gtctcgctca tgcggttctc ctcgcgctat tcgtcctcat cctcgtcgtc
      301 tccgctcccc gcgacaccgt agctcttgag cttacgagcg agcgtgtttc ggctgactcc
      361 cagctgccga gccgcctcac tgcgattgcc ctcgcattgt tccaagcgcc ggagaatgtg
      421 ctgcttttcg atctcatcca gcgtcatcgg cgcggtattg gtctctgccg gagcaggatt
      481 cgagacttcc ggtgggagat cttccggttt gatcgtctcg tcgtccgaaa ggagaatcgc
      541 gcgttcgatc gcgtgctgga gctcgcgcac attcccgggc caacgatacc gcgtgagaag
      601 cgacacggat tcgggtgaga gcttgaagcc ctgtcgtgag aacttctcct gatatcgtac
      661 gatgaagtgt tgcgcgagcg gcaaaatatc ctcggtgcgt tcgcggagcg gagcgatgcg
      721 aatggggaca acgcggagac gatagtataa atcctctcgg aacgtgccct cttcaatcgc
      781 cttttcgagg tcgcggttcg tcgatgcaag cacgcgcacg tcggcggcga tagggcgcgt
      841 ttcgccgacg cgccgaatct ctcccgattc cagcacgcgc agaagcgctg cctgcgcgcc
      901 cagttccatg tcaccgattt catcgaggag tatcgtaccg ccgtctgcgt cttcgaagag
      961 tcctttcttc gcggtgatgg cgcccgtaaa cgcgcctttc gcatgtccga aaagctccga
     1021 ttcgagaagc gtatcggtca atgcgctaca gttcaatgtc aaaaagagtc cgtcggcgcg
     1081 ggacgaacgg ttgtgaatcg aacgcgcgac gagttctttc cccgtcccac tctcaccggt
     1141 gatcagaacg gtgacatcgg tgcgcgcaat acgctgaatc gtccggaaaa cgtgctgcat
     1201 cggcttgctc gtgccgatga ggtcgtcgag ctgcaactgc tcaaccttcc cacggagaac
     1261 ttcgagttcg ctcagaagct gacggtgttc gagcacgcgt tcgagaacga ctttcagctc
     1321 gttcgggtcg accggcttgg tgagatagtc gctcgcgcca agtttcatcg cctcgacggc
     1381 cgaatcaatc gagccatgcc ccgtcatgat cagcaccgcc ggtgcgggat accgatgccg
     1441 tgcggcacga agcacgtcga gcccgtcgac gcgatccatt cggagatcgc tgattacgac
     1501 atcaaagact tcttcggaaa ggcgatcgac cgcctcctga ccggaattcg ccgcgtcggc
     1561 ttcatagccg gcgtctctca acgcgagaga gagcagggtt gttaccgacc gttcgtcgtc
     1621 aacgaggagt actttcggtg agggcattca cgttatccgt tggaagtgaa atagaaaccg
     1681 tcgtgccttc gccgacgcga ctcttgattt gtatcgatcc atcgtgcccc tggataatcc
     1741 gccgcgcgat tgccaatcca aggccggttc cgcgcggttt cgtggtatgg aacggttcaa
     1801 acacgcgcga aagttcctcc tgcggaatcc cgccaccggt atccgtgatg tcgagcgaaa
     1861 catactcgct cgactccggt tgtattcgca ccgttagcgt acccgaaccg ttcatcgcct
     1921 gcaaaccgtt caagatgatg ttccacaata cctgccgaat ctgatccccg tccacggaca
     1981 catttggaag atcgttcggc cattctgtct cgataaccac attgccgacc gcatcggaat
     2041 caccttcgat gatgcggac
//
LOCUS       DSCC01000020            2305 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031684, whole genome shotgun sequence.
ACCESSION   DSCC01000020 DSCC01000000
VERSION     DSCC01000020.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031684"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..853)
                     /locus_tag="ENN56_00170"
     CDS             complement(<1..853)
                     /locus_tag="ENN56_00170"
                     /inference="COORDINATES: protein motif:HMM:PF00501.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDS73931.1"
                     /translation="MSETLGSMLRETARSFPQKPAIIYEGETTSYGRLDTMSDAIAAY
                     LHRQGVELGDRVGLYCINSPEFIASYYGIAKVGAVVVPINLLLGSEEVAYILADAGVR
                     GLIYHDAYAKTAHTVSESVPTLRVRIVVGKRSVDGEVTLADVLSESATTFESPTINAG
                     NLASVMYTSGTTGKPKGAMLTHGNLIANASAIRDAMKVTSDDVFLVVLPMFHSFAATV
                     GTHLPVMTGATISAMLRFIPDDVGKTIEETKATVFLGVPSMYTVLANLPEGRRADLSS
                     LRIAVSGG"
     gene            1023..1883
                     /locus_tag="ENN56_00175"
     CDS             1023..1883
                     /locus_tag="ENN56_00175"
                     /inference="COORDINATES: protein motif:HMM:PF04909.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73932.1"
                     /translation="MVIDAHNHVYWYGYGPDELVANMDEHGIDVTWLLSWELAEGMYD
                     HGYDKTFMTPYIGMPLEAIWVAVDKYPSRFVPGFAPNPKLPHAVERLRAAHRNGARVA
                     GEFKFRCLYDDPDAIAVFRAAGELGMPVTLHIDVPWLPPSGSRNAVTWWYGGTIDNLG
                     NALRLCPDTVFLGHAPGFWREVSGDADNDPAAYPNGPVVPGGRLRPLFDEYPNLWADL
                     SAGSALTAISRDRDWGRQFLIDYQERILFARDYFDGRLMEYLNSLELPENVLTAILSG
                     NAMKLTPLRE"
BASE COUNT          503 a          605 c          659 g          538 t
ORIGIN      
        1 agccgccaga taccgcgatt cgaagcgatg agagatcggc acgacggcct tccggtagat
       61 ttgcaagaac ggtatacatc gaagggacgc cgagaaaaac cgtcgccttt gtttcttcaa
      121 tcgtctttcc gacatcatcc gggatgaacc ggagcattgc ggatattgtc gcaccggtca
      181 tgaccggaag atgcgtcccg acggtcgcgg cgaatgaatg aaacatcggc agaacgacca
      241 aaaacacgtc gtcggaagtg actttcatcg catcacgtat tgcagaagcg ttagcgatga
      301 gattaccgtg cgtgagcatg gcaccttttg gtttgccggt agtgccggat gtatacatca
      361 cggacgcaag atttccagcg ttgattgtcg gggattcgaa agtcgtcgcg gattcgctga
      421 gaacgtccgc aagggtcact tctccgtcga ccgaccgttt cccgacaaca attcgtactc
      481 gcagtgttgg aaccgattcg gatacggtat gcgccgtctt cgcataggcg tcgtggtaga
      541 tgagaccccg aactccggcg tctgcgagaa tgtacgcgac ctcttcagac ccgaggagca
      601 gattgatcgg cacaacgact gcaccgactt tcgcgatgcc gtagtaggac gcgataaatt
      661 caggggaatt tatacaatac aacccgacgc gatcgccgag ttcaacgcct tgacgatgca
      721 agtatgctgc gatcgcgtct gacatcgtgt ctaatcgtcc gtacgatgtc gtctcgcctt
      781 cgtagatgat tgcgggtttc tgtgggaatg agcgtgcggt ttcgcggagc atcgacccga
      841 gtgtttccga catggccact tctccgtgta tcgaggttcc ataccgttct cagtcaagat
      901 acacagcggc gacgggcaca gaaaagggaa cgagaatatc gcgatgttgg accgcgcgtt
      961 ttcctcttgt agcttcacaa ttcgcgtgaa caatggatac ttcgtttcct atgaggcttt
     1021 gaatggtaat tgacgcacat aaccacgtgt actggtacgg ttacggtccc gacgagcttg
     1081 ttgcaaacat ggacgagcac ggcattgatg tgacgtggct gctcagttgg gaactcgccg
     1141 aaggaatgta tgatcatggg tatgacaaga cgtttatgac tccatacatc ggaatgccac
     1201 ttgaagcgat ctgggtcgcc gtcgataagt acccgagtcg gtttgtaccg gggttcgcac
     1261 cgaatccgaa attgccacac gccgtagagc gtttgcgtgc tgcgcatcga aacggtgctc
     1321 gcgttgcggg agagtttaag ttccgctgtc tctatgatga tcccgacgca attgcagtgt
     1381 ttcgcgcggc cggcgagctt ggcatgcccg tgacgttaca tatcgatgtg ccttggttgc
     1441 caccgtcagg ttcccgaaat gccgtgacgt ggtggtatgg tgggacgatt gataatttgg
     1501 ggaacgcgct tcgactgtgt ccggacacag tgtttctggg tcacgcgccc ggtttttggc
     1561 gcgaagtgag tggtgatgcg gacaatgatc ccgctgcgta cccgaatggg ccggtcgtcc
     1621 cgggtggaag gttacggccc cttttcgacg aatatccgaa cctttgggcc gacctcagtg
     1681 cgggtagcgc tttgacggcg attagtcgtg accgagattg gggccggcaa ttcctgatcg
     1741 actaccagga gcggatattg ttcgcacgtg attactttga tggccgccta atggaatacc
     1801 ttaactcgct ggaactgcca gaaaacgtac tcacggcgat tctgtcggga aatgcgatga
     1861 agctcacgcc gctacgggaa tagcggatac tacgacgaat cacgcatcca cagtttgagc
     1921 ggatgctcac ggttcgtcag tggtagagcg actttcgtcc cacttcgcgc gctgacaaat
     1981 gccgcgtgat tgatttccat tgcggctcga ccgtcatacc ccgagcaacg tgggcgttcg
     2041 ttcttttcga tagcgcggat caagtcacgc atcatgaatt gagtgcccca tcggttctcg
     2101 tcgaagatgc gtcggtcgga atcggagaga ggcacatcaa tcgcgttcca gtcgatcgga
     2161 tcgttcacct ttccaccacc accgacagtg cggtacaaga cgggatcggt gccgcgaatt
     2221 gccagtacgg ctttcgttcc gactacttcg aagccgtacg tcccaacgcc ttcccagtac
     2281 tcagatacgc cggttacacc accat
//
LOCUS       DSCC01000021            1729 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046914, whole genome shotgun sequence.
ACCESSION   DSCC01000021 DSCC01000000
VERSION     DSCC01000021.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046914"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(198..776)
                     /locus_tag="ENN56_00180"
     CDS             complement(198..776)
                     /locus_tag="ENN56_00180"
                     /inference="COORDINATES: protein motif:HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73933.1"
                     /translation="MRFTWGLALLLMGAIGVADAQERFVPVLGVAAREYGVSGMYSQI
                     AMTDTTDYSDFAITGRYAYSLGFGLQLEAETGYTRFRTDVLDSTGQSWSIAGNAIMNI
                     PLMSERLSAFGLVGYGYTRTWTSAVYPAPVDKITSKWSHRFVQYGFGAKYMLVANAGI
                     RVDYRWINPSGVPTGVSDRTWRQMLIGISVFQ"
     gene            complement(780..1691)
                     /locus_tag="ENN56_00185"
     CDS             complement(780..1691)
                     /locus_tag="ENN56_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDS73934.1"
                     /translation="MAITNRPNLDPREHIIVKEDDSPIMQKALRTQQVLFIDDVKKSG
                     FLPDEKTEQRSYRKHHSVCLPLKVEDRPIGVLNLNDFRSEDRIQEYINIAVLAAAYLA
                     PVISNAMLYSQIEETANRDGLTQLYNRGYFDRSINENFRRSKRYGRPLSLLFLDIDHF
                     KRFNDVHGHQAGDTVLRRVAAQMSETMRQGIDIVARYGGEEMVLIAPETGTDGIMILA
                     ERIRFDIEQMKVTIESENEKKDLSVTVSIGTSTLLPGTHDFPNERALIEAADMALYEA
                     KHRGRNRCMHFTPSDVEQARASESKGA"
BASE COUNT          403 a          479 c          450 g          397 t
ORIGIN      
        1 gtacatgagc acgtagaacg gcactactcg gacaagaaca acgataaaca gcggcgtagc
       61 gcgcgtcgcc gaaaagcgaa gcgcgtcacg agtgagttca atgagagcgg ggaatggatg
      121 cacgatatgc ctgaacgaga aagcgcatta ccgaagttga gaaccgctcc gtcgcgtttg
      181 acggagcggc acggcgatta ctgaaacaca gatataccaa tcagcatctg tcgccacgtt
      241 cgatcagaca caccggtggg aacgcctgac ggattaatcc agcggtaatc gacgcggatg
      301 ccggcattgg caacgagcat atacttcgca ccgaacccgt attgaacgaa ccgatggctc
      361 cacttcgatg tgattttatc taccggggcc gggtagacgg cgctcgtcca ggttcgtgta
      421 tatccatacc cgacgagacc gaacgcgctg agacgctcgg acatgagcgg aatattcata
      481 atagcgtttc ccgcgatact ccaagattga cctgtactgt cgagaacatc cgtgcgaaat
      541 cgcgtgtatc ccgtttccgc ctcgagttgc agaccgaaac caagcgagta cgcgtaacga
      601 cccgtgatag cgaaatcgct gtaatccgtc gtgtcggtca tggcgatctg cgaatacatg
      661 ccgctcacac cgtattcacg tgcagcgacg ccaagtaccg gtacgaaccg ctcctgcgcg
      721 tcagcaactc cgatggcacc catgagaagg agcgccagcc cccatgtgaa tcgcattgct
      781 caggctcctt tcgactccga cgcgcgtgct tgctccacgt cgctcggggt gaaatgcatg
      841 catcgatttc ttccacggtg tttcgcttcg tacaacgcca tatcggctgc ttctatcaat
      901 gctcgctcgt tgggaaagtc gtgtgttcca ggtaataacg tcgatgtgcc gatgctcacg
      961 gtcacactga gatctttctt ctcattttcg gattcgatgg tcaccttcat ctgctcaatg
     1021 tcgaaccgga tacgttctgc gaggatcatg ataccatccg tacctgtttc tggtgcgatg
     1081 agcaccatct cctcaccacc ataccgcgcg acaatatcaa taccttgtcg catcgtttcc
     1141 gacatttgag ccgccaccct ccggagaacg gtatcacctg cttggtgccc gtgcacgtcg
     1201 ttgaatcgct tgaaatgatc gatatcgaga aataacaacg ataacggccg accgtatcgt
     1261 ttcgatcggc ggaagttttc gttgatcgat cggtcgaaat agccgcggtt gtaaagttgt
     1321 gtcagtccat cgcggttcgc cgtttcttca atttgagaat acagcatcgc gttggagatc
     1381 accggcgcga gatatgccgc agcgagaacc gcaatgttga tgtattcctg gatgcgatct
     1441 tccgaccgaa aatcattcaa gttcagtaca ccgatcgggc ggtcttcgac tttcagcgga
     1501 aggcacacgg aatggtgctt ccgatagctc ctctgctccg tcttctcgtc cggcaggaaa
     1561 cccgacttct tcacatcgtc gataaacaga acttgctgtg tacgcagagc cttctgcata
     1621 atcgggctgt cgtcttcctt gacaatgatg tgctcgcgag gatcgaggtt cggacgattc
     1681 gtgattgcca atcggaactc gcgggattcg ccgtggtaaa gaaatagac
//
LOCUS       DSCC01000022            1800 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044461, whole genome shotgun sequence.
ACCESSION   DSCC01000022 DSCC01000000
VERSION     DSCC01000022.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044461"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(25..255)
                     /locus_tag="ENN56_00190"
     CDS             complement(25..255)
                     /locus_tag="ENN56_00190"
                     /inference="COORDINATES: protein motif:HMM:PF13411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDS73935.1"
                     /translation="MNDRGHRSDTPNTHRISTQEVCRLLDISKRTLQNWEKSGVIPRA
                     PRDWRGYRIFTEQDVEDIRRVIQQKAERNRAT"
     gene            complement(477..1673)
                     /locus_tag="ENN56_00195"
     CDS             complement(477..1673)
                     /locus_tag="ENN56_00195"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13181.4,HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS73936.1"
                     /translation="MKSITMMIGRMCFAAVLGGLILSAAATAQETNELGLTEEQALAY
                     NAALDEAQQAASQRKFREATEAYRRALEIYADDFLTYFNMGISFQSLRQEDSAQVYFN
                     HAIEKNPHFGPARIALANSHLKMDQVQDAETVFVALRDQLADSIEYVLAAEQGLYNVA
                     IKYTNLAVAELNNRDYDTAEQYSNRAIELAGDVFRPYYIAAMVQERKNKDEDARRLWD
                     SALARANSDEEKALALNGIGRMEISTAKAAVRANNDAAARRARIQAVEHLRESTDLDP
                     NRSSSWINLGNTLFDLGRYDEALDALTRAEELERRDFRIPSKIADTYIQLGDCAAAEA
                     AASRAIALQRTNANAHAVRAEALECLGRKREAITEYETAARDPRWRQRATYQAKKLRE
                     ELGISN"
BASE COUNT          353 a          524 c          497 g          426 t
ORIGIN      
        1 aggcaaattt gatacccgat ttcgctacgt cgctcgattc cgttcagcct tctgctggat
       61 tacccgccgg atatcctcca cgtcttgctc ggtaaagatg cggtatccgc gccaatcgcg
      121 tggcgctctc ggtatcactc cggacttctc ccagttctgc agcgtccgct tcgaaatatc
      181 gagaagccgg cagacctcct gggtggatat ccggtgtgta ttcggcgtat cggaccgatg
      241 tccgcggtca ttcatcccga tcacctcacc ccgtcgagcg acgtggtact ttcatgggtt
      301 actatagcaa gcatagaatc aatagtagcc aatgtcaagt gaacgttaag gcgacgctct
      361 gtgttagggg aaaggcaagt cattcacttg gcgtgccggt gccgtatcgc gggtacaaaa
      421 aacgggcggc aaccgtcatg gtcaccgccc gttcctcaga tactcattgg cgagagttag
      481 ttgctgatac cgagttcttc gcggagcttt ttcgcctggt atgttgcgcg ctgacgccac
      541 cgcggatcgc gtgcggccgt ctcgtattcc gtgatggctt cacgcttccg accaaggcac
      601 tcgagcgctt cagcgcgaac cgcgtgcgcg ttggcattcg tgcgctgaag tgcgattgcg
      661 cggctcgccg ctgcttcagc cgccgcgcaa tcaccaagct ggatgtaggt atcggctatt
      721 ttggacggaa tccggaagtc acggcgctcc aactcttcgg cacgagtaag cgcatccaat
      781 gcttcgtcat atcgaccgag gtcgaacagc gtgttcccga gatttatcca actcgatgaa
      841 cgattgggat caaggtccgt cgactcccga aggtgctcga cagcttgaat gcgcgcgcga
      901 cgtgccgccg cgtcattgtt cgcacgcact gcggctttcg ccgtcgaaat ctccatacgg
      961 ccgattccgt tcagtgcgag cgctttctct tcgtcgctat tggcgcgagc gagagcgctg
     1021 tcccacagtc tccgagcgtc ttcatccttg ttcttccgct cctgcaccat cgcggcgatg
     1081 tagtatgggc ggaagacgtc gccggccaat tcgattgcgc gattcgagta ctgttccgcc
     1141 gtatcgtaat cccggttgtt gagttccgcc accgcgagat tcgtgtactt gatagcgacg
     1201 ttgtagagac cctgttccgc tgcgagaacg tattcgatcg aatccgcaag ttggtcgcga
     1261 agtgcaacga acacggtctc ggcgtcctgg acttgatcca tttttaggtg ggaattcgcg
     1321 agggcgattc gagccggacc gaaatgcggg ttcttctcga ttgcgtgatt gaagtatact
     1381 tgcgcactat cttcctgccg tagcgactgg aagctgatgc ccatattgaa atacgtaagg
     1441 aagtcatcgg cgtaaatctc caacgcgcgt cgataggctt ccgtcgcctc gcggaattta
     1501 cgctgcgaag ccgcttgctg cgcttcatcg agggcggcgt tatacgccaa tgcctgttcc
     1561 tcggtaagac cgagttcatt tgtttcctgg gcggtcgccg cagccgacag gatcagtccg
     1621 ccgagaaccg ccgcgaaaca catgcgaccg atcatcatgg taatgctctt cataaacaca
     1681 actccatggt atcgcgtgat gtggacgcga acgttaggga cacccggcgt tacctacctc
     1741 ggttcctttg acgaaaacgc tcgaaaacat gtagcgaact cacgtcgaac gcaagccccc
//
LOCUS       DSCC01000023            1836 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043307, whole genome shotgun sequence.
ACCESSION   DSCC01000023 DSCC01000000
VERSION     DSCC01000023.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043307"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..309
                     /locus_tag="ENN56_00200"
     CDS             <1..309
                     /locus_tag="ENN56_00200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HDS73937.1"
                     /translation="RAKMPEETTELPFSVDGSVVLLVDDVLFTGRTVRAAIETLLDFG
                     RPAEIQLAALIDRGHRELPIMANYIGRTVQTDRADTIRVRLKEIDDEDHVILIRSENP
                     "
     gene            306..1262
                     /locus_tag="ENN56_00205"
     CDS             306..1262
                     /locus_tag="ENN56_00205"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDS73938.1"
                     /translation="MSTWNRRHLLGMTDLSRKDITDLLDLAGQFAGVLDRPVRSVPTL
                     RGWTVVNLFFEESTRTRISFELAEKRLGADVVNFSASASSVKKGESLRDTARNIEAMK
                     IDAVVIRHKSVGAPYYLTQQIDAAVINAGDGMHEHPTQALLDLMTIRHHFDRIEGLRI
                     AIIGDILHSRVARSDLWGMTTMGADVVMCGPPTLLPKHTETFPGTWTTDINEALDGAD
                     VVYALRIQRERQESGLFPSIREYTQTYGITTDRMKLAKPHAVVMHPGPVNRGWELSDD
                     VVDAENSLILRQVTHGVAVRMAVLYLTAGRDPGSIQIGKHTE"
     gene            1312..>1836
                     /locus_tag="ENN56_00210"
     CDS             1312..>1836
                     /locus_tag="ENN56_00210"
                     /inference="COORDINATES: protein motif:HMM:PF01979.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDS73939.1"
                     /translation="MGNIILRGGHLIDPSQGIDEPKDVVIADGKIAKIISPGAKASGR
                     EIDVTGHIVAPGLVDIHVHLREPGYEYKETIATGAEAAVAGGVTSVVAMPNTDPPPDT
                     ADAVRGFYKRAETAACHVYTTGAITRSRKGEQLAELADLAAAGVVGFSDDGDPVGNTR
                     TLLHAMEYSQPIGLP"
BASE COUNT          377 a          530 c          600 g          329 t
ORIGIN      
        1 cgcgcgaaaa tgcccgaaga gacgacggaa ctcccgttca gcgtggacgg ctcggtcgtg
       61 ctccttgtgg acgacgtgct gtttaccggt cgtacggttc gcgcggcgat tgagacgctc
      121 ctcgatttcg gacgtcccgc ggagatacag cttgcagcgc tgatcgaccg cggacaccgc
      181 gagcttccca tcatggcgaa ctacatagga cgaacggtgc agactgaccg ggcggacacg
      241 atacgcgtcc gtctcaaaga gatcgacgac gaggatcatg tcatcctcat tcgttcggag
      301 aatccatgag tacgtggaat cgacggcatt tgctcgggat gaccgacctg agccgcaagg
      361 acatcaccga cctcctcgac ctcgcgggcc agttcgccgg tgtcttggat cgaccggtgc
      421 gaagtgttcc gacattgcgt gggtggacgg tggtcaacct gttcttcgag gaaagcaccc
      481 gcacgcgcat atcgttcgag cttgcagaga agcgccttgg tgcggacgtg gtgaacttca
      541 gcgcgtcggc ctccagcgtg aagaagggcg agtcgcttcg cgataccgcg cgaaatatcg
      601 aggcgatgaa gattgatgcg gtggtcattc gccataaatc ggtcggcgcg ccgtattacc
      661 tgacgcaaca gatcgacgcg gcggtgatca atgcgggcga cgggatgcac gaacatccaa
      721 cacaagcgct gctcgatctg atgacgattc ggcaccactt cgaccggatc gaagggctgc
      781 gtatcgcgat tatcggcgat attctgcaca gccgcgtcgc tcggtccgac ctgtggggca
      841 tgacgaccat gggcgcggac gtggtcatgt gcggcccgcc gacgttgctc ccgaagcata
      901 ccgagacgtt ccccggcacg tggacgaccg atatcaacga agcgctcgac ggcgcggatg
      961 tggtctatgc gctgcgtatt cagcgcgaac ggcaggaatc gggcttgttc ccgtcgattc
     1021 gcgagtacac gcagacgtac ggcattacta cggaccggat gaagcttgcg aagcctcacg
     1081 cggtggtgat gcatccgggt ccggtgaatc gcggatggga actcagcgac gacgttgtgg
     1141 atgcggagaa ctcgctgatc ctccggcagg tgacacacgg cgtcgcggtt cgtatggcgg
     1201 tgctctacct taccgccggc cgcgatcccg gcagtatcca gatcggcaag cacaccgaat
     1261 gattctcgaa atatccgcaa taggcgtgtg agaagattct acggggacac catggggaat
     1321 atcatactgc gcggcggcca cctgatcgac ccgtcacaag gaatcgacga gccgaaggat
     1381 gtggtcatcg ccgacgggaa gattgcgaag atcatctcgc cgggcgcgaa agcatcgggg
     1441 cgtgagatcg acgtaaccgg acatattgtt gcgccggggc tggtggatat ccacgtccac
     1501 ctgcgcgaac ccggctacga gtacaaggaa acgattgcga cgggtgcaga ggcagccgtt
     1561 gcgggcggtg ttacgagcgt cgtcgcgatg ccgaacactg atccgccgcc tgacacggct
     1621 gacgcggtgc gcggattcta caagcgcgcc gagacggcag cgtgccatgt ctacacgaca
     1681 ggcgcgatta cgcggtcgcg caaaggcgag cagttggcgg aactggcgga tttggcagcg
     1741 gcgggcgtcg tcgggttcag cgacgacggc gatccggtcg gcaacacccg cacgctcctc
     1801 cacgcgatgg aatattcgca gccgatcggt ctaccg
//
LOCUS       DSCC01000024            2498 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028507, whole genome shotgun sequence.
ACCESSION   DSCC01000024 DSCC01000000
VERSION     DSCC01000024.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028507"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1498
                     /locus_tag="ENN56_00215"
     CDS             <1..1498
                     /locus_tag="ENN56_00215"
                     /inference="COORDINATES: protein motif:HMM:PF01663.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73940.1"
                     /translation="PNILLITGDEWRADALGCAGNPVVQTPNIDRLAARGVRFENAYS
                     PAPMCVPTRVSILTGQVPRAHGCITNGIVPSDNRTIVELLREAGYRTGAFGKMHFVPI
                     YADLGFDTMQLAEQHGAGWQIDEYHRWLYEEYGMVDWIDVWDQVSDFRAKAPDWFHET
                     YGVLKSPIPEEAYHTNWITDRFVDWIGTNEATEPWFAWVSYIKPHHPFDPPGKYADMY
                     DPADIPLPPKDDRSNKPLLAGFDPKRAHYDISAWTDDDIRRMTALYYGNITLIDDCIG
                     RMMEALTASGDADTTVVIVTSDHGDFLGHRGLITKTPFVLYEDTIRVPLVIADPTIDT
                     PCRVRDAFVNLIDLFPTIAKRTGIQHDHRIHGHDLGPVIRGEVGYTSQIAVSETSGQI
                     AIRKGDWKLIQTRDGKAELYNLEDDPYEIRNRMPELRDSAIVADLRARALEYVLSTEW
                     DRLKRDTDGVQVIVDDEAKKLGRGKLCINPNAWCDQWPEEFTPKKRTS"
     gene            complement(1509..2048)
                     /locus_tag="ENN56_00220"
     CDS             complement(1509..2048)
                     /locus_tag="ENN56_00220"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDS73941.1"
                     /translation="MARIYTRNGDTGNTRLPGGEQVSKDHVRLRVSGSLDELMAHIGV
                     VRALNAHPEIERLLEHVQVHLFELGTDLSNPVVSESRIKADDVTHLEREIDRLAESCP
                     ALRAFILPGGTSVAAHLHVSRAVCRRAERDIVALAAVSDIPKTAIPYINRLSDLLFTL
                     ARFGNAEAGVSDTEWKPRS"
BASE COUNT          597 a          680 c          694 g          527 t
ORIGIN      
        1 accaaatata ttgctaataa caggagatga gtggcgcgcc gacgctcttg gatgcgcggg
       61 gaatcccgtc gttcagacgc caaatatcga ccgattggcg gcacgcggtg tgaggtttga
      121 aaacgcgtac tcgccggcac cgatgtgtgt cccgacgcgc gtatcaatac tcaccggtca
      181 ggtcccacgt gctcacggtt gtatcacgaa cggaatcgtt cccagtgaca accgcacaat
      241 cgtcgaactc ctgcgcgaag ccggctatcg gaccggtgcg ttcgggaaaa tgcatttcgt
      301 accgatttat gccgacctcg gtttcgatac gatgcagctt gccgaacagc acggcgccgg
      361 atggcaaatt gacgaatacc atcggtggct gtacgaagaa tacggtatgg tcgattggat
      421 tgacgtttgg gatcaggtat cagacttccg cgcaaaggca ccggattggt ttcatgaaac
      481 ctacggcgta ctgaaatcgc ctatccccga agaagcgtac cacacgaatt ggattaccga
      541 tcgtttcgtc gattggatcg gaacgaacga agccactgaa ccgtggttcg cgtgggtatc
      601 gtacatcaaa ccgcatcacc cgttcgatcc gccgggtaaa tacgccgata tgtatgatcc
      661 ggccgatatc ccgttaccgc cgaaggacga ccggtcgaac aagccgcttc ttgccggatt
      721 cgatccgaag cgcgcacact acgacatatc agcgtggaca gacgacgaca tccgtcggat
      781 gacggcgctg tactatggaa acatcacact catcgacgat tgcatcggga gaatgatgga
      841 agcgcttaca gcgagcggtg acgcggatac cacagtcgtg atcgtcacgt cggatcacgg
      901 agattttctc gggcaccgcg gactgataac caaaacgccg tttgtgttgt acgaggacac
      961 gatacgtgtg ccgctggtca tcgccgatcc aaccatcgat acgccgtgta gggtacgcga
     1021 tgcgttcgtc aaccttatag acctctttcc caccatcgca aaacggacgg gaatccaaca
     1081 cgatcaccgt atacatggcc atgatttggg tccggtcata cggggagaag tcggatatac
     1141 ctcccaaatt gcagtatcgg aaacaagtgg tcagatagcg attcgcaaag gtgactggaa
     1201 gctcatacaa acacgcgatg gtaaagcgga actgtacaac ctcgaagatg atccgtacga
     1261 aattcggaat cgaatgcccg agttacgtga ctccgccatc gttgcggatt tacgtgcgcg
     1321 cgcgcttgaa tacgtattgt caacggagtg ggaccgtttg aaacgcgata cggacggcgt
     1381 tcaggtgatt gtcgacgacg aggcgaaaaa gctcgggcga ggcaaactgt gcatcaatcc
     1441 aaatgcgtgg tgcgatcagt ggccggaaga gtttacgcca aaaaaacgta cctcatagcg
     1501 tgtttggtct acgaacgcgg tttccattcg gtatcggaaa caccggcctc ggcgttcccg
     1561 aatctggcga gcgtgaagag caagtccgag agccgattaa tgtacggaat cgcggttttc
     1621 gggatgtccg atacggcagc cagtgcaacg atatctcgtt ccgcacgtcg gcatactgcg
     1681 cgagacacgt gaaggtgcgc cgcaactgaa gttccacccg gtaagatgaa tgcccgtagt
     1741 gcggggcacg attccgctaa tcggtcaatc tctcgttcaa ggtgcgtgac gtcatctgct
     1801 ttgatacgtg attccgacac gaccggattc gagagatcgg taccgagttc gaataggtga
     1861 acctgcacgt gttcgagtaa ccgttcaatc tcaggatgcg cgttgagtgc gcgaacgacg
     1921 cctatgtgcg ccatgagctc gtccaacgag cccgatacgc gaagtcgaac gtgatccttt
     1981 gaaacctgct ccccgccagg tagtcttgtg tttcccgtgt caccgtttcg cgtgtaaatc
     2041 cgagccatat gacacacctc gttatgccgc accaagcggt gcgagttcct cgtcggtcgc
     2101 ttttcgaatc aagtcgccaa atttctcgcg ttcgaatgat tccggatgaa gcacggcagc
     2161 aagtaattcg aggccatcga taagactcgg tgatgctcgg tgtaatgaaa gacgaccatc
     2221 cacgaggtag acttcgcccg actgaacagc aggtatgctc gtccactctg agcgctttcg
     2281 gagaagcggt agttcgccaa cggtatcgtc gaggtcgaac gccgtaagtg cgaatatgag
     2341 cacgtcgggt ttcgagtcaa cgatgtcctt ccacggagtc tgaacagccg gagcctgtct
     2401 cgttccgaca accggtgttc cgcccgccgc ctcaatcagc tccggtagcc acatgccgcc
     2461 tgctaccggc ggatcaaccc actcgagtag cgcaactc
//
LOCUS       DSCC01000025            1862 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042483, whole genome shotgun sequence.
ACCESSION   DSCC01000025 DSCC01000000
VERSION     DSCC01000025.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042483"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="ENN56_00225"
     CDS             complement(<1..1595)
                     /locus_tag="ENN56_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73942.1"
                     /translation="MVARVILLATLLVPFAFAPPAIAETDDFGPYHPILVDWDAKRAD
                     GYYTSTNSSTLAWAEAYVLNAFMAGYRLTGDTYWLGRIADHTDTLLANLRDVPESVPY
                     DTSIYADGYRGWGSTGYYAGDYDEYIVHDGCVGSAIAEYIAEVFNNPDELWDEFGDRA
                     KAHFDTLSYHVAGKWLANWDADRYNASTYGTVLRHYGGFERLPHNQANAFGSFLVFLN
                     DVVNSPKWAELNGEISPDYYMPYIDEMAAFLKNDLRVGAGDSYQWGYWPGGGDEDVSH
                     ANITVEFAWICYKRGIVFDETDMKRFANTMSKLMWNGSFENVGINKYNRPGADYAWTN
                     YIWSWAYLSEADPFLFHIYHNFFRHRTSFSYYDIAGAIRNWLNADLAGGLNYPITIRV
                     EDDGDGVARPGETVTLHVAFKNYGVAPDTSDYVYVDLESVFPNDGVFVLIDVDDDPLV
                     IEPDSTAELSVTVTIPADFSGAAIRPIVSARGMLGGEPREMSKEFTVTSGNPAFLQVT
                     PDPPTNLPYDADISSFLPKARHT"
     gene            complement(1761..>1862)
                     /locus_tag="ENN56_00230"
     CDS             complement(1761..>1862)
                     /locus_tag="ENN56_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDS73943.1"
                     /translation="CEPACPVTAIFAEEDVPEEWQEYIEKNYDHYGK"
BASE COUNT          433 a          522 c          497 g          410 t
ORIGIN      
        1 cacgtgtgcc gcgcctttgg aaggaagctg ctgatgtctg catcatacgg aagattcgta
       61 ggaggatcgg gcgttacttg cagaaaggcc ggattcccgg aagtcacggt aaactctttc
      121 gacatctccc gtggttcccc accgagcatt ccgcgcgcag acacgatcgg ccgtatcgcc
      181 gcaccactga agtccgctgg aatcgtgacc gtaaccgaca gctctgcggt actgtccggt
      241 tcgatcacga gcggatcgtc atcgacgtcg atcagaacga aaacaccgtc attcgggaac
      301 accgattcca aatcgacgta cacatagtcc gaagtgtccg gcgcgacacc gtagttcttg
      361 aatgcgacgt gaagcgtaac cgtttcgccc ggacgagcca caccgtcacc gtcatcctca
      421 acacgaatcg taatcggata attgagaccg ccggcgaggt cggcgttcag ccagttgcga
      481 atggctcctg cgatgtcata atacgagaat gatgtacggt gccggaagaa gttgtggtag
      541 atgtggaaga ggaacggatc cgcttcggat agatacgccc aactccatat gtaattcgtc
      601 catgcgtaat cagcgcctgg cctattgtat ttgttgatac caacgttttc gaaactcccg
      661 ttccacatga gtttcgacat cgtgttggcg aatcgcttca tatcagtttc gtcaaacacg
      721 attccgcgct tgtaacatat ccaagcaaac tcaaccgtga tatttgcgtg cgatacgtcc
      781 tcatcgccac caccgggcca gtacccccat tggtacgagt cacccgctcc gacgcggagg
      841 tcgttcttga gaaacgcagc catctcatcg atgtacggca tgtagtaatc gggtgatatc
      901 tcaccgttca gttcagccca tttcggcgaa ttgactacgt cgttcagaaa gacgaggaat
      961 gagccgaaag cgtttgcctg attgtgtggt aaccgctcga agcctccata gtggcgtaac
     1021 accgtgccgt aggtactcgc gttatagcga tctgcgtccc agttcgcgag ccatttcccc
     1081 gcaacgtgat aggagagcgt atcgaaatgc gccttcgcgc ggtcaccgaa ttcgtcccag
     1141 agttcgtcgg gattgttgaa tacctcggcg atatattcgg cgatcgcgct tccgacacag
     1201 ccgtcgtgaa cgatgtattc atcgtagtct ccggcgtagt aacctgtact gccccatcca
     1261 cgatacccgt cagcatatat cgatgtgtca tacggcactg actccggcac gtcgcgcagg
     1321 ttcgccagca gcgtatctgt atgatcggcg atgcgaccca gccagtacgt atcgccggta
     1381 agtcgatacc cggccatgaa cgcattgagc acgtacgcct ctgcccaggc gagagtgctc
     1441 gagttcgtcg acgtgtagta cccgtcagct cgtttcgcgt cccaatccac aagaatcggg
     1501 tggtacggtc cgaaatcgtc ggtctcggcg atcgctggcg gcgcaaacgc aaacgggacg
     1561 agcagcgtgg caagcaagat aactcgtgct accatgaaaa aacctccggt acgacacgta
     1621 ggcgcggcgt tgtttcaagg gagtaagata cggttatacg cagtaagacc aatgcagaag
     1681 ccggaaaaac ggaacaagcc cacacctgcg acgtgaagcg agatgtggac ctgatcggaa
     1741 atggtgaagc gatgtagcgc ttatttcccg tagtggtcgt aattcttctc gatatactcc
     1801 tgccattcct ccggaacgtc ttcttctgcg aagattgccg tcacgggaca cgcaggttcg
     1861 ca
//
LOCUS       DSCC01000026            1665 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1049429, whole genome shotgun sequence.
ACCESSION   DSCC01000026 DSCC01000000
VERSION     DSCC01000026.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1049429"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            133..>1665
                     /locus_tag="ENN56_00235"
     CDS             133..>1665
                     /locus_tag="ENN56_00235"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDS73944.1"
                     /translation="MHSRNLTWTSGIALAAILAVFAVFMVYPLFYAIKGAFMVNGEFS
                     LEFFGLMFTNPVHREAIWNSTLVGLTVTAATTVVALPVAFVMVRREFPGRALFNGLLL
                     VPMVMPPFVGAVGMQQLLARYGSINLLLIKLGLMNEAAPYDWLGTGFWAVVVLEVLHL
                     YPIMYLNVSAALANVDPSLEEAAQNMGASGPRLFRTITMPLMMPGYFAGAIIVFIWAF
                     TDLGTPLIFNYSRVVPVVIFDAVGDIHENPMGFAMVVLVLALTVFFFLLSKKLLGAGS
                     SHQMMSRGHVIGAQKPMSGRFVPVAWLGLIVLVAFALLPHISVLLSSIADRWFMSVLP
                     TAYTGKYYDAVFTHKLTISSIRNSFFLSSLSTVFDIVIGVGIAYLLARKVFPGKDLLD
                     ALAMLPLVLPGLVLAFGYVGSFSGTFLDVKVNPMPLLVIAYAIRRLPYMIRAAYAGFQ
                     QTSEALEEASLNLGAGPARTLRKITLPLVFANIVAGAVLAFAFAMLEVSDSLILAFRE
                     EYY"
BASE COUNT          278 a          503 c          491 g          393 t
ORIGIN      
        1 cgtcggtcga gcgaccgccg atagagtgat cgtcggtcga gcgaccgccg gttcagtaac
       61 cgccgatacc agccgaatcg tcagtcgaaa taaatcggcg gatacgtatc cgcactgtat
      121 acagggagtt tcatgcacag ccgcaacctc acgtggacat ctggaattgc gctcgccgcg
      181 attctcgccg tgttcgccgt gttcatggtg tatccgcttt tctacgcgat caaaggcgcg
      241 ttcatggtca acggcgagtt ctcgctcgag ttcttcggac tcatgttcac gaatccggtg
      301 caccgcgagg cgatctggaa tagcactctc gtcgggctta cggtcaccgc ggcaacgacc
      361 gttgtcgcgc tacctgtcgc gtttgtgatg gtgcgccgtg agtttcccgg tcgcgcgtta
      421 ttcaatggcc tgctgctcgt tccgatggtg atgccgccgt tcgtcggcgc agtcggtatg
      481 cagcaacttc tcgcgcggta cggctcgatc aaccttctcc tcatcaagct cgggttgatg
      541 aacgaggcgg ctccgtacga ttggcttgga accggattct gggccgtcgt cgtgctcgaa
      601 gtgctccatc tgtatccgat catgtacctc aacgtctccg cagcgcttgc gaacgtcgac
      661 ccgtcgttgg aagaagcggc gcagaatatg ggcgcatcgg gtccgcgcct gttccgcacg
      721 ataacgatgc cgctcatgat gcccgggtat ttcgccggcg cgatcatcgt gtttatttgg
      781 gcgttcacgg acctcggcac gcccctgatc ttcaactact cgcgcgttgt gcctgtcgtg
      841 atcttcgacg cggtggggga tattcacgag aatcccatgg gattcgccat ggtggtcctc
      901 gtactcgcgc tcacggtatt cttctttctc ttgagcaaga agctcctcgg cgccgggtcg
      961 agccatcaga tgatgtcgcg tgggcacgtg atcggcgcgc aaaagccgat gagcggtcgg
     1021 ttcgtccccg tcgcttggct cggactgatc gtactcgtcg cgttcgcgct tctgccgcac
     1081 atctccgtgc tcctctcgtc aatcgcggac cggtggttca tgtcggtgct tccgacagca
     1141 tatacgggta aatactacga cgccgtattc acgcacaaac tcaccatctc cagtattcga
     1201 aacagcttct tcctcagctc gctcagcacg gtattcgaca tcgtgatcgg cgtcgggatc
     1261 gcgtacctcc tcgcacggaa ggtcttcccg gggaaggacc tgctcgacgc gttggcgatg
     1321 cttccgctgg tactgccggg gctcgtgctc gcatttggat acgtcggctc gttcagcggc
     1381 acgttcctcg acgtgaaggt gaacccgatg ccgctgctcg tgatcgcgta cgcgatacgc
     1441 cggttaccct acatgattcg ggcggcgtat gcgggattcc agcagacaag cgaggcgctt
     1501 gaagaggcgt cgctcaacct tggcgcgggt ccggcgcgaa cgctgcggaa gatcacgctg
     1561 ccgctcgttt ttgcgaatat cgtcgcggga gcggtgctcg cattcgcctt tgccatgctc
     1621 gaggtgtcgg acagcctcat tctcgcattc cgtgaggaat attac
//
LOCUS       DSCC01000027            2228 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033246, whole genome shotgun sequence.
ACCESSION   DSCC01000027 DSCC01000000
VERSION     DSCC01000027.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033246"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..609
                     /locus_tag="ENN56_00240"
     CDS             <1..609
                     /locus_tag="ENN56_00240"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS73945.1"
                     /translation="TNGREGWEGISTRKPDIVLSDIAMPEMDGVELCRRIKTTESTSD
                     IPVIMFTSLKEAEIQLAGTSAGADAYLSKDTDPRILKARIEALLASRTRQSESTEKRV
                     DSARRQTLSQTVVTLAHHLNNSLISMHATASVVNPDNTSDSRKLRDVCITETRKMLVV
                     LKALKQMADERELKTTVYVGSEVMFDLETELARLADATEKPS"
     gene            650..>2228
                     /gene="polX"
                     /locus_tag="ENN56_00245"
     CDS             650..>2228
                     /gene="polX"
                     /locus_tag="ENN56_00245"
                     /inference="COORDINATES: protein motif:HMM:PRK08609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDS73946.1"
                     /translation="MNAKEIAGVFTEMAALLELTGANPFRARAYQNVARALEGVTEDI
                     ETLVREDRLGEVSGVGKGLAASIAELVTTGSLKDYDELREKVPDGVLEILELPGLGPK
                     RVRRIWEELGVTNIGELEYACRENRLAALDGFGEKTQTNVLKAIAFRVQGRDRHLYHR
                     ARAAADAVLELVKSQPGVIRVEIAGSLRRHRETIGDLDILASASDDARPGIMDAFVKN
                     DDIVDVIAHGSTKSSVRLKSGINADLRVVSDEEFPFALAYFTGSKEHNTVLRGRAKRE
                     GLKLNEYGLFRENSDTIPCENETNLYRALGLPYIPPELRENMGEFEAAERNELPDLVT
                     MDDIRGILHVHTVRSDGVHTVEQLVQAAKTLGAEYLGIADHSRSAFYAGGLSENDVRA
                     QWDEIDEVNERIDDITVLKGIESDILSDGGLDYNDELLAGFDFVIASVHSRLHMAEDE
                     ATERLLRAIDHPATTILGHPTGRLLLARDGYPVDMQRVIERCVERGVAIELNASPHRL
                     DLDWRWCRNVREHGGVIA"
BASE COUNT          542 a          591 c          659 g          436 t
ORIGIN      
        1 acaaacggcc gtgaaggatg ggaggggatc tccactcgga aacccgatat cgttctctcg
       61 gatattgcga tgccggaaat ggacggcgtt gaattgtgcc gtcgaatcaa gacaacggaa
      121 tcaacatcgg atattccggt tatcatgttc acgagcctca aggaagcgga aatccaactc
      181 gcgggcacga gcgccggtgc tgacgcgtat ctctcgaagg acaccgatcc ccgcatactt
      241 aaggctcgaa tcgaagcctt gctcgcctct cgaacgcgac aatccgaatc cacggagaag
      301 cgagtcgatt cggcgcggag gcagacgctc agccaaacgg tcgtcacact tgctcatcat
      361 ttgaataaca gtctgatctc catgcacgcc acggcgagtg ttgtaaatcc cgataacaca
      421 agtgattcgc ggaagcttcg agacgtctgt ataacagaaa cacggaagat gctcgtcgtc
      481 ttgaaggcgt tgaagcagat ggcagacgag cgggagctca agacgacggt ctacgtggga
      541 tctgaagtaa tgtttgatct tgagacggag cttgcacgac tcgccgacgc gacagagaag
      601 ccgtcatgag tcgcaaacac acacggatca aatcacggtt cgaatcgata tgaatgcgaa
      661 agaaatcgcc ggcgttttca cggaaatggc cgcgttactc gaactgactg gtgcgaatcc
      721 cttccgcgca cgcgcctatc agaatgtagc gcgggcactc gagggtgtta ccgaggacat
      781 cgaaacgctt gttcgcgaag accggctcgg cgaagtgtcc ggcgtaggga aaggcctcgc
      841 agcgagtatc gcggagttgg tcaccaccgg ctcactcaaa gactacgatg aactccgcga
      901 gaaagtcccg gacggcgtgc tcgagatact ggaactcccg ggactcggtc cgaagcgagt
      961 tcgccgtatc tgggaagagc ttggcgtgac gaatatcggc gagcttgagt acgcgtgtcg
     1021 cgagaatcgg ctcgccgcac tcgatgggtt tggcgagaag acccaaacga acgtgctcaa
     1081 ggccatcgcg ttccgcgtgc aagggcgcga tcggcatctt taccatcgcg cgcgagccgc
     1141 ggcagatgcc gtcctcgaac tggtcaaatc ccaaccgggt gttatccgtg tggaaatcgc
     1201 gggaagcctc cggcgacacc gagagacaat cggtgacctc gatatactcg caagcgcttc
     1261 cgatgacgca cgacccggaa tcatggatgc gttcgtgaaa aacgacgata tcgtcgatgt
     1321 aatcgcacat ggctcgacga aatcgagtgt ccgactaaag agtggaatca acgccgatct
     1381 tcgcgtcgta tccgatgagg aattcccatt cgcgcttgca tacttcaccg gatcgaagga
     1441 gcacaatacc gtcctgcgcg gacgtgcaaa acgcgaaggg ttgaagctca acgagtacgg
     1501 tctctttcgc gagaacagcg atacgatccc gtgcgaaaac gagacgaatt tgtatcgagc
     1561 gcttggtctg ccgtacattc ctccggagct tcgtgaaaac atgggagagt tcgaagccgc
     1621 ggagcgaaat gagctaccag atctcgtcac catggacgac attcgtggga ttcttcacgt
     1681 gcacacggtg cgttcggacg gggtacacac ggtcgaacag cttgtgcagg cggcgaaaac
     1741 gctcggtgcc gagtacctcg gaatcgccga tcacagtcga tcggcattct acgcgggtgg
     1801 actgagcgag aacgatgtcc gcgctcagtg ggatgagatc gacgaggtca atgagcggat
     1861 tgatgatata accgtgctca aaggtatcga aagcgatatt ttatcggatg gcggattaga
     1921 ctacaacgac gagctacttg cggggttcga cttcgtcatc gcgagtgtgc acagccgact
     1981 ccacatggct gaggacgaag caaccgagcg gcttttacgc gccattgatc atccggcaac
     2041 gacgatcctc gggcatccga cagggcgact tcttctcgca cgtgatgggt atcccgtcga
     2101 catgcaacgc gttatcgaga gatgcgttga acgtggtgtt gcgatcgaac tgaatgcctc
     2161 accgcaccgt cttgacctcg attggcgctg gtgtcggaat gttcgggagc acggtggtgt
     2221 tatagcaa
//
LOCUS       DSCC01000028            1861 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042493, whole genome shotgun sequence.
ACCESSION   DSCC01000028 DSCC01000000
VERSION     DSCC01000028.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042493"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..775
                     /gene="rlmD"
                     /locus_tag="ENN56_00250"
     CDS             <1..775
                     /gene="rlmD"
                     /locus_tag="ENN56_00250"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:PF13649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDS73947.1"
                     /translation="RHDGLLRHLVVREARTTGEVMVNLVVARDDFATELTEAVPTVAS
                     VLVSVNTRRGDTAIAESTRVLAGKPTITERIGDIEIELSPESFVQTNTIGAARLYDIA
                     AERAGLTGDERVLDLYCGTGLIGLWVRDQAREVVGVESVPEAVADAARLRDRLDANAV
                     RFVTGLAEDELPKWTVSGEQFDVAFVDPPRMGVHPKALTALVELAPRRIVYVSCNPRS
                     LARDVATLCDAGYVPGSIQPVDMFPQTAHVECVTVLNRA"
     gene            843..>1861
                     /gene="aspS"
                     /locus_tag="ENN56_00255"
     CDS             843..>1861
                     /gene="aspS"
                     /locus_tag="ENN56_00255"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDS73948.1"
                     /translation="MEYLNGLSRTHRCGDLRIDDVGAEVVLMGWVLSARDHGGVIFVD
                     LRDRGGLTQVVFDPRESAEVHASAERLRSEYVIAVTGRVRPRMEGMANPKLETGEIEV
                     ICSQLRILNVAEPPPIPIEGAPANEDARLKYRYVDLRRVDMQRNLTLRSIAYQATRSY
                     LHQEGFMEVETPFLVRSTPEGARDFIVPSRVSPGKFFALPQSPQIYKQLLMVSGFDRY
                     FQIVKVFRDEDLRADRQPEFTQIDLEMSFVEENDVMTIAEGITRSVFRDTIGYDIGEA
                     VPRLDYDEAMSRYGSDKPDTRFGLELVDVTGIVRESEFKVFRAVVESGGLVSAINVKG
                     GASFSR"
BASE COUNT          387 a          484 c          602 g          388 t
ORIGIN      
        1 ccgacacgac ggactgctcc gtcatctcgt cgtacgggaa gcacgtacca ccggtgaggt
       61 gatggtgaat ctcgtcgttg cgcgcgatga tttcgcaacc gagttgaccg aagcggtgcc
      121 gaccgtggcg agtgtgctgg tgagcgtgaa tacgcggcgc ggcgacacgg cgattgcaga
      181 gagcacacgc gtgcttgcag gtaagccgac gataaccgag cgtatcggcg atatcgaaat
      241 agagctgtcc ccggaatcgt tcgtccagac aaacaccatc ggagctgcgc gcttgtatga
      301 tatcgccgcg gaacgagccg gactgaccgg cgacgaacgc gtgctggatc tctattgcgg
      361 gacgggcctc atcgggttgt gggtgcgaga tcaggcgcgt gaggtcgtcg gtgtggagag
      421 tgttccggaa gctgtcgccg atgcagcgcg gttgcgtgac cgactcgacg caaatgcggt
      481 gcgattcgtt accggcctcg ccgaggacga gttgccgaaa tggacggtat ccggcgagca
      541 gttcgacgtt gcgtttgtcg atccgccgcg tatgggagtc catccaaagg cgcttaccgc
      601 attggtcgaa ctcgccccgc gtcgtatcgt ttacgtttcc tgtaatccac gctcacttgc
      661 acgagatgtc gcgacactat gtgatgccgg atatgttccg ggatcgattc aaccagtgga
      721 tatgttcccg cagactgcgc acgtcgagtg tgtgacggtg ctgaacaggg cgtagatttc
      781 gagtggataa tataaatcga acaaccggaa atggagtgag ggtatcaatg agcgaaccga
      841 agttggagta tctgaacgga ttgtcgcgta cccatcggtg cggagatttg cggatcgatg
      901 atgttggcgc ggaagtggtg ctcatgggtt gggtgctgag cgcccgcgat cacggcggcg
      961 tgatcttcgt cgacctgcgt gaccgaggcg gcctgacaca ggttgttttc gacccgcgtg
     1021 agagcgccga ggtccatgct tccgccgaac gacttcggtc agaatacgtc atcgcggtaa
     1081 cggggcgcgt gcggccgcgt atggaaggta tggcgaaccc gaaactcgag accggtgaga
     1141 tcgaggtgat ctgtagtcaa ctacgaatcc tcaacgtcgc cgagccgcct ccgattccga
     1201 ttgaaggtgc accggcgaat gaagacgcac gtctgaaata ccgctacgtg gacctccgtc
     1261 gggtggatat gcagcgcaat cttacgcttc gcagtattgc gtaccaggcg acgcgtagct
     1321 acctccatca ggaagggttc atggaggtcg agacaccgtt tctcgtccgc agtacaccgg
     1381 aaggcgcgcg cgatttcatc gtccccagcc gtgtttcgcc gggaaagttt ttcgcgttac
     1441 cgcagtcgcc gcagatttac aagcaactcc tcatggtgag tggcttcgat cggtacttcc
     1501 agattgtaaa ggtattccgc gacgaagatt tgcgcgcgga ccgtcaaccg gaattcacgc
     1561 agatcgatct tgagatgtct ttcgtcgagg aaaacgacgt catgacaatc gcagaaggca
     1621 tcacgcggtc ggtgttccgc gacacgatcg gctatgatat cggtgaggcg gtaccgcgcc
     1681 tcgactatga cgaggcaatg tcacggtatg gctcggataa accggatacg cgattcgggc
     1741 ttgaactggt cgatgtgacg ggaatcgtgc gcgagagcga gttcaaggtg tttcgcgccg
     1801 tggtagaatc cggcgggctc gtaagtgcaa taaacgtgaa aggcggagcg tcgttcagcc
     1861 g
//
LOCUS       DSCC01000029            1809 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044191, whole genome shotgun sequence.
ACCESSION   DSCC01000029 DSCC01000000
VERSION     DSCC01000029.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044191"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(189..851)
                     /gene="radC"
                     /locus_tag="ENN56_00260"
     CDS             complement(189..851)
                     /gene="radC"
                     /locus_tag="ENN56_00260"
                     /inference="COORDINATES: protein motif:HMM:PRK00024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDS73949.1"
                     /translation="MRKQRTELPPDPPLLLLQRHGPAGVSDADLLALVLGSARAPIST
                     AHTIAEQYPLERLVTLPYDELRRVPGLTEPQAAALAASIELAKRGLDRGLGGLPKLLR
                     PSDVAKIVSDLGTERQEHFVCLYLNARTQLIHRATISVGTLNGSLVHPREVFARALEH
                     NAAAVILVHNHPSGDPTPSSDDLNLTQRLIEAGLIMGIEVIDHIIVTADRWISLKQTG
                     EL"
     gene            complement(877..1272)
                     /locus_tag="ENN56_00265"
     CDS             complement(877..1272)
                     /locus_tag="ENN56_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73950.1"
                     /translation="MIEGRIVRIIDERTLIANVGSVAGVSVGDEFVVIQSMDQVVDPE
                     TNEELGTWEMIKARLIAIHVQSRLTTLVPLPGEVSRQTVLSERLAWGSHGAPIGSQDV
                     SLAVDRSQVSGRRRVDLIRVGDHIQSVDE"
     gene            complement(1269..>1809)
                     /locus_tag="ENN56_00270"
     CDS             complement(1269..>1809)
                     /locus_tag="ENN56_00270"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS73951.1"
                     /translation="YRVGGNYRMPAMCAALLRVQLARYPEAHAKRTENGTYLAQRLSE
                     IPGLEPTVVSPKVTRHAWHLFIWRYDAKAFDGVSRDAFLEAMRKEGVPAYQGYSIPLT
                     EQPVFAEKRFDLSHPARNVDFTEIAVPVAKRACESEACWLTQNVLLGSKADMDDIVTA
                     ARKIYENRHELVKRSETAS"
BASE COUNT          341 a          538 c          550 g          380 t
ORIGIN      
        1 atttgccgct cggccataca tcgtgttccg tccatgggtg accactcaat gcaataaccg
       61 atgtgctgtg gtcggacaac gcatactcct ttcctacaga ttgctatcag acgctgctcg
      121 gatacgatcg atgcggtctg aagtactcgt gacgccgacg gtggggacgt ccgtcggtac
      181 ggacgccgct acaactcgcc ggtttgtttc aggcttatcc agcggtctgc tgtgacaatg
      241 atatgatcga tgacctcgat ccccatgatc aagcccgctt cgatcaggcg ctgcgtcagg
      301 ttgaggtcat cactcgatgg cgtcggatct cccgacgggt ggttgtgtac aaggataact
      361 gcggcggcgt tgtgctccag cgcgcgcgca aagacttcac gcgggtgtac gagcgatccg
      421 ttcagcgtgc cgacgctgat cgtcgcgcga tgaatgagct gcgtgcgtgc gttgagatac
      481 aggcagacga aatgctcctg ccgctccgtg ccgaggtcgg agacaatctt cgcgacatcg
      541 gatggacgaa gcagcttcgg caggccgcca agcccgcgat cgagaccgcg tttcgcaagc
      601 tcgatagaag ccgcgagcgc agccgcctgc ggctccgtca ggccgggaac tcgacgcaat
      661 tcgtcgtagg ggagcgttac caaacgttcg agcgggtact gttcggcgat ggtgtgagcg
      721 gtgctgattg gcgcccgagc cgatccgaga acaagcgcga ggagatcggc gtcgctcact
      781 ccggccggac cgtgtcgttg gagtaagagc agcggcggat ccggtgggag ttcggttcgc
      841 tgctttctca ttattctcta ccccggctga tcgaattcat tcgtcgacac tctgtatgtg
      901 gtctccgacg cgaatcagat caacgcggcg acgaccggat acctgtgagc gatccaccgc
      961 gaggcttacg tcctgcgaac cgatcggcgc gccgtgagaa ccccacgcga ggcgctcgct
     1021 gagtaccgtt tgccgcgaaa cttcgcctgg cagcggaacg agtgttgtga gccgcgattg
     1081 cacgtgaatg gcgatgagtc gcgctttgat catctcccag gtgccgagtt cctcgttcgt
     1141 ttcgggatcg acgacctggt ccatggattg aatgacgacg aactcgtcgc cgacggatac
     1201 gccggcgacc gaaccgacgt tcgcgatcaa cgtgcgctcg tcgatgatcc gaacaatacg
     1261 accttcgatc atgatgcagt ctccgatcgt ttcaccagct catgccgatt ttcgtagatc
     1321 ttccgcgcgg cggtcacgat gtcgtccatg tcggctttcg agccgagaag cacgttctgc
     1381 gtgagccagc acgcttcgct ttcgcacgcg cgcttagcga ccggcacggc aatctcggta
     1441 aaatcgacat tccgtgcggg atggctcaga tcgaagcgct tttcggcgaa cacgggctgt
     1501 tcggtcaacg gaatcgagta accttgatac gccgggacgc cttccttgcg catcgcctcg
     1561 aggaaggcat cacgcgatac cccgtcgaac gccttcgcgt catatcgcca gatgaacagg
     1621 tgccacgcat gacgcgtcac cttcggcgat accacggttg gctcaagccc ggggatctca
     1681 ctaaggcgtt gtgcgagata cgttccgttt tccgtccgtt tcgcgtgtgc ttccggatat
     1741 cgcgcaagtt gcacacgcag caacgccgcg cacatggcgg gcatgcggta gtttccgccg
     1801 acgcggtag
//
LOCUS       DSCC01000030            6322 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1008023, whole genome shotgun sequence.
ACCESSION   DSCC01000030 DSCC01000000
VERSION     DSCC01000030.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1008023"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            420..809
                     /locus_tag="ENN56_00275"
     CDS             420..809
                     /locus_tag="ENN56_00275"
                     /inference="COORDINATES: protein motif:HMM:PF09527.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDS73952.1"
                     /translation="MCGGITNTSEHPRITCRNVLTGSPRTHGEAVIAVTSRPNDPGKR
                     RNNRGVSSLVEAMRAAGPLFGLGIQFAIMVGLGVFVGHWLDQRYEWDPWGALIGGIVG
                     IALGFYQFFRAVSSGTDKSEKRNDDST"
     gene            833..1336
                     /locus_tag="ENN56_00280"
     CDS             833..1336
                     /locus_tag="ENN56_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73953.1"
                     /translation="MNNTKSPAPHTPAENESNVGKKPIAPLGVDHEAHRRAVLVRTHW
                     LRWFFGLSAVFIVAAIVIAIAYRSGAAQQLLIGWAISFVMCLLSGWLAFGSIGRSMNR
                     IMLVAFGGMLLRMVIVGASVAVAVVTHTSYLVFFVAGLMGGYVLYQALEIFMLKSVVR
                     IASGATE"
     gene            1333..2256
                     /gene="atpB"
                     /locus_tag="ENN56_00285"
     CDS             1333..2256
                     /gene="atpB"
                     /locus_tag="ENN56_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit A"
                     /protein_id="HDS73954.1"
                     /translation="MTYRSIFLALGIVAATLVGGPALAADPHGDDHSNTVDPIHHVVD
                     ANYLEIPWPNAHLAKKIYLPTLVTVHFTDDVQIELAITKHVVVMWIVAILLIVFVTGI
                     GRKASADKAPTGTVNFLEVFVVWMRDNVTVPAMGPHWGRKLLPYFLTVFFFILSGNLF
                     GLIPYSATFTGNLAVTAAMASTSFILIQAIAIREAGFGGWIKHLTAGVHPALWPIMIP
                     VEFLGLFTKPFALAVRLFANMSAGHIVIVSLLGLIFIFGHVAVAGLSVPFAVFIYLLE
                     ILVAFIQAFIFTMLTAVFTGMGVHSHHAEAH"
     gene            2347..2559
                     /gene="atpE"
                     /locus_tag="ENN56_00290"
     CDS             2347..2559
                     /gene="atpE"
                     /locus_tag="ENN56_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_660926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDS73955.1"
                     /translation="MAYWAAGIGAGFVTFGAGLGIGKIAAAAMEGTARQPEAGGQIQT
                     SMLIAAALIEGIAMFGQVINVLLAIK"
     gene            2656..3150
                     /gene="atpF"
                     /locus_tag="ENN56_00295"
     CDS             2656..3150
                     /gene="atpF"
                     /locus_tag="ENN56_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HDS73956.1"
                     /translation="MLVSVNPGLIIWTIVTFLILVFVLGKWGWGPIIKALEGRESHIH
                     EALSEAEKARAEARELREQYDGLIARAQDESREIIQDGRDTAEKLRREMEANSRAEAA
                     RVLEQARNDIKNAKDAALREIRDTVADLATEAAGKIVQKNLDAEGHRSMVDDLIDRLP
                     ETKR"
     gene            3201..3776
                     /gene="atpH"
                     /locus_tag="ENN56_00300"
     CDS             3201..3776
                     /gene="atpH"
                     /locus_tag="ENN56_00300"
                     /inference="COORDINATES: protein motif:HMM:PF00213.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HDS73957.1"
                     /translation="MAIHSRASIRYARALLMAADTTDQIENLTGEVDGLRRALSGSDD
                     LVAFLGNPTIEVETKKQVLEQVFADKLSPMMWRFINLLVEKRREPLFGDILEATHGLL
                     DERAGRVRASVRTAQPLADTQQERLREQLAKITGKEVSLAVEDDPSLVAGFVARVGDT
                     VYDASLRAQLERLRNRLKSADLGAAASETGS"
     gene            3805..5340
                     /locus_tag="ENN56_00305"
     CDS             3805..5340
                     /locus_tag="ENN56_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDS73958.1"
                     /translation="MTFDVRPDEVSKILAHQIADFDRGVDVYDTGTILQVGDGIARVH
                     GLQNVKAGELVEFPDGVMGLAFNLEEDNVGCILFGGETDLKEGDQVRRTGNILQVPVG
                     EALLGRVVTPLGTPLDGKGEIVSEHFGLVERKAPGVITRKPVDTPLQTGLKAVDAMTP
                     IGRGQRELIIGDRQTGKTALAIDTIINQKGHNVFCIYVAIGQKGSTVANVIRVLEEHG
                     AMEYTTIVSATAAEPAPLQYLAPYAGATMGEYFRDSGRDALIVYDDLTKHAWAYRQVS
                     LLMRRPPGREAYPGDVFYLHSRLLERAARLNEDFGGGSLTALPIIETQEGDVSTYIPT
                     NVISITDGQIFLETDMFLSGQRPAVNAGISVSRVGGNAQIKAMRTVAGQLRNELARYR
                     EVAAFAQFGSDLDEVTQRIIRRGERTQEMLKQGQYQPMPVEEQVVTILAATSGVLNDI
                     PVHAVKQWESEYLDMMRAQQSEILKEIRETKALSPETREKVLDIMRTFNKSFKAAERR
                     AGS"
     gene            5380..6258
                     /gene="atpG"
                     /locus_tag="ENN56_00310"
     CDS             5380..6258
                     /gene="atpG"
                     /locus_tag="ENN56_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDS73959.1"
                     /translation="MPNLRMIRRRITAVQNIQQVTRAMRMIAASRLRRAQEGIEQTRP
                     YADQLTEFIRHLAAQVDQESHPLLEQREVKKATIIVIAGDRGLCRGFNANAFRKAIHR
                     SEELAAQNIETDFIVLGKKTVDFFRRRRLPVVEAFPGIFQHLDFATATAVARLATERF
                     LNGETDLVEIVYNEFVSVLRQNLVTRQYLPLKATPDESEESANEYDEYIYEPDQEYIW
                     RALLDKYLNFAVWRMLLESNAAEQAARMTAMEEATNNAEEMIKNLISVRNKVRQTSIT
                     TELTEIVGSAESLRAD"
BASE COUNT         1345 a         1801 c         1906 g         1270 t
ORIGIN      
        1 agcttgactt catgaccatg gtgatacctc cgcagtacaa gggagttcac agagtacact
       61 gccatatcga gcactcgtcg gcgtcacgcg atgacaccga ctgagtatac gctgccctgt
      121 cgatgaatat ccaccagatc gggcaaacgg tcaaggaagc gacacaaaca cgccgccttt
      181 gcgtcggtaa ctgactgtag aatcagtgtc atcgccatat ctcgtctatt tttgcgcagt
      241 actggtgtaa cagggtgcaa tcgaattgcg tcgacctaca attgacgtgc gtcgatcctt
      301 tatcgacgtg cgtcaccgtg ctcccttacg cgtgcgcgat gcgtaaaaag aaatattgcg
      361 tgtacggtac cgaaaggggt tgaccgattc ggggagaaac tccatacttt tgaccttgta
      421 tgtgtggggg gattaccaac acttcagagc acccgcgcat tacctgtcgg aacgtgctga
      481 caggctctcc ccggacacac ggagaggcgg tgatcgccgt gacttcccga ccgaacgatc
      541 ccgggaagcg ccggaacaac cggggtgtgt caagccttgt cgaagcgatg cgtgccgctg
      601 ggccgctatt cggtcttggc atccagtttg ccatcatggt gggcctcggc gtcttcgtcg
      661 gacattggct cgatcaacgg tatgaatggg acccgtgggg cgcgctcatc ggcgggatcg
      721 tcgggatcgc gctgggattc taccagttct ttcgtgcggt atcatcggga actgacaagt
      781 ccgagaaacg caacgacgac agcacgtagc tcggacaggg gccgacggaa cgatgaacaa
      841 cacgaaatca ccggcgcccc atactccggc cgagaacgag tcgaacgtcg ggaaaaagcc
      901 catagcaccg ctcggcgtgg atcatgaagc gcatcggcgc gcggtattgg tgcgaacaca
      961 ctggctgcga tggtttttcg ggttgtcagc cgtgttcatc gtcgcagcga ttgtcattgc
     1021 catcgcgtat cgtagcggtg cggcgcagca attgctcatc ggctgggcta ttagcttcgt
     1081 catgtgtctg ctgagtggtt ggcttgcgtt tgggtcaatc ggccgatcga tgaaccgcat
     1141 catgctggtg gcgttcggcg gaatgctctt gcgtatggtg attgtcggcg catcggtggc
     1201 ggtcgcagtt gtcacgcata catcatacct cgtgtttttc gtcgccgggc tgatgggcgg
     1261 atatgttttg tatcaagcac ttgagatatt catgttgaaa tctgtggtac gaattgcatc
     1321 cggagctacg gaatgacgta tcgttccata ttcctcgcgc tcggcatcgt cgccgcgaca
     1381 ctggtcggtg ggcccgcgct tgccgccgat ccgcacggag acgatcatag caacacggtg
     1441 gacccgatcc accacgtcgt cgacgcgaac tacctcgaga ttccgtggcc caacgcacat
     1501 ctcgcgaaga aaatctacct cccgactctt gtaaccgtcc atttcacgga cgatgtacaa
     1561 atcgaattgg caatcacgaa gcacgtggtc gtgatgtgga tcgtcgcgat cctgctgatt
     1621 gttttcgtga ccggaatcgg gcggaaggcg tctgcagaca aggctccgac cggcaccgtc
     1681 aacttcctcg aagtcttcgt cgtctggatg cgtgacaatg tcaccgtgcc ggcgatggga
     1741 ccccattggg ggcggaaact gctcccgtat ttcctgacgg tgttcttctt cattctctcc
     1801 gggaacctct tcggtctcat tccctacagc gcgacgttta ccggcaatct cgccgtcacc
     1861 gccgcaatgg cgtccacgag cttcatactc attcaggcga tcgccattcg cgaggcggga
     1921 ttcggcggct ggatcaagca tcttacggcg ggcgtccatc cggcgctctg gccgatcatg
     1981 ataccggttg agttccttgg gctgttcacg aagccgttcg cacttgccgt tcgtcttttc
     2041 gcgaacatgt ccgccggaca tatcgtcatc gtatcactgc tcgggctgat cttcatcttt
     2101 ggacacgttg cggtagcagg gctgagcgtg ccctttgcgg tattcatcta cctgctcgag
     2161 atactggtag cattcatcca agcgttcatc tttaccatgc ttacggcagt ctttacgggc
     2221 atgggcgttc actcacacca cgcggaagcg cactgacatg tgtagcggtg caacagagcg
     2281 ccgcgcacgg ctttcaggag gagtttgact gtggaagaag ctctcagcag catcggcaac
     2341 atcggtatgg cctattgggc cgctggtatc ggcgctggat tcgtcacgtt cggcgcaggt
     2401 ctcggcattg gaaagatcgc tgcggccgcg atggaaggta ccgctcgtca gccggaagca
     2461 ggcggacaga ttcagacctc gatgcttatc gcggcggccc tgatcgaagg tatcgccatg
     2521 ttcggccagg ttatcaacgt tctcctggca atcaagtaag tatctcaagt cccgcttcgt
     2581 cggtataccg cggatcggcc gtcgcttccg gcggccgtcc ggcgtgctga gcggatcggg
     2641 acgcggagcg caccgatgct ggtaagtgtc aatcccggcc tgattatctg gacgatcgtc
     2701 acgttcctca tcctcgtgtt cgttctcggc aaatggggct gggggccgat catcaaggca
     2761 ctcgagggac gcgaaagcca catacacgaa gcgctctccg aggcggagaa ggctcgtgct
     2821 gaagcgcgcg aactgcgcga acagtacgat ggtctcatcg cccgcgcgca agatgagtca
     2881 cgcgagatca tccaagatgg acgcgatact gcggagaagc tccgtcgtga gatggaagcc
     2941 aattctcgcg cggaagcggc tcgcgtgctc gagcaggcgc gtaacgacat caagaacgcc
     3001 aaggacgctg cgctccgcga gattcgcgac accgtcgccg atcttgcgac cgaagcggcg
     3061 ggcaagattg ttcagaagaa cttggacgcg gaaggtcacc gctcgatggt ggacgatctg
     3121 atcgatcgcc tgcccgaaac gaaacggtaa cctacgtccg agtagaggca cattgaacgc
     3181 agcgcgttca cggagtacgt atggccattc attcgcgagc gagcatcaga tacgcacgcg
     3241 cgttgctcat ggccgccgac actaccgatc agatcgaaaa cctgaccggc gaagtcgacg
     3301 gtttacggcg tgcgctgagc ggatccgacg atctcgtagc gtttcttggg aatcccacga
     3361 ttgaggtgga gaccaagaaa caggtgctcg aacaggtatt cgcggacaag ctctcgccaa
     3421 tgatgtggcg ctttatcaac ctgctcgtgg aaaagcgtcg tgagccgctc ttcggggaca
     3481 tcctcgaagc gacacacggg ctgctcgacg agcgcgcggg acgtgttcgc gcgtcggttc
     3541 gcacggcgca accgcttgca gatacgcagc aggaacggtt acgggaacaa cttgcgaaga
     3601 tcaccggcaa ggaggtcagc ctcgcggtag aggatgatcc ttcgcttgtg gccgggttcg
     3661 tcgcgcgtgt cggagacacg gtgtacgacg catcgctccg agcgcagctt gagcggctcc
     3721 gcaatcggct gaagtcagcc gatcttggcg cggctgcgag cgaaactgga tcgtgattca
     3781 caagaggaaa gacctgtcat ggcaatgacc ttcgacgtgc ggccggatga ggtatcgaag
     3841 attctcgccc accagatcgc cgatttcgac cggggtgtgg atgtctatga taccggaacc
     3901 attctgcagg tcggtgacgg aatcgcccgc gtgcacggat tgcagaacgt caaggccgga
     3961 gagctggtag agtttcccga cggcgtcatg gggttggcgt tcaacctcga agaagacaac
     4021 gtcggctgca ttctcttcgg cggcgaaacg gacctgaagg aaggcgatca ggttcgccgt
     4081 accggaaaca tcctgcaggt acccgttggc gaagcgctgc tcggtcgcgt agtcacgcca
     4141 ctcggtacgc cactggacgg caaaggcgag atcgtctccg aacacttcgg tctcgtcgaa
     4201 cggaaggcgc ccggcgtcat tacccggaag ccggtggata cgccgctcca gaccggcctc
     4261 aaggccgtcg acgccatgac gccgatcgga cgtggacagc gtgagttgat cattggcgac
     4321 cgtcagaccg gtaagaccgc gctggcgatt gatacgatca tcaaccaaaa gggccacaac
     4381 gtcttctgca tctacgtcgc catcgggcag aaaggctcga cggtggcgaa cgtcatccgc
     4441 gttctggaag agcatggcgc gatggaatac accaccatcg tcagcgcaac cgccgcggaa
     4501 ccggccccgc ttcagtatct tgcgccgtac gccggcgcaa ccatgggcga gtacttccgc
     4561 gattccggtc gcgacgcgct cattgtgtac gacgacttga cgaagcacgc gtgggcgtat
     4621 cgacaggtgt cgttgctcat gcgtcgccca ccgggacgtg aagcgtatcc cggcgacgtg
     4681 ttctatctgc actcgcgcct gctcgagcgc gccgcccgtc tgaacgaaga cttcggcggt
     4741 ggatcgctga cggcgcttcc aattatcgaa acgcaggaag gcgacgtctc gacctacatc
     4801 ccgacaaacg tcatctccat taccgatgga cagatattcc tcgagaccga catgttcctc
     4861 tcgggtcaac gtcccgcggt gaatgccggt atttcggtat cccgcgtcgg tggaaacgcg
     4921 cagatcaagg ccatgcgcac cgttgcgggt cagcttcgta acgagcttgc gcgctaccgc
     4981 gaagtcgccg cgttcgcgca gtttggttcc gacctcgatg aagtgacgca gcgaatcatt
     5041 cggcgtggtg agcgcacgca ggagatgttg aaacaggggc agtatcagcc gatgcccgtc
     5101 gaggagcagg tcgtcacgat tctggcggcg acgagcggtg tgctcaacga cattccggtc
     5161 cacgccgtga agcagtggga atcggaatac ctcgacatga tgcgcgcaca gcagagtgag
     5221 atcctcaaag agattcgcga gacgaaagcg ctttcgccgg aaacccgtga gaaggtgctc
     5281 gacatcatgc gaaccttcaa taaatcgttc aaggcagcgg agcgtcgtgc cggttcgtaa
     5341 ttccggcgaa gaacgtaacg cttcgaaacc gagggcagca tgccgaacct gaggatgatc
     5401 cggcggcgga ttaccgccgt gcagaacata cagcaggtga cccgcgccat gcggatgatt
     5461 gccgcatcgc gtctgcgtcg cgcgcaggaa gggattgaac agacccgacc gtatgccgat
     5521 caactcaccg agtttatccg gcatctcgcg gcgcaggtcg atcaggagag tcatccgctg
     5581 ctcgaacagc gcgaagtgaa aaaggcgacg atcatcgtta tcgccggtga ccgtggcctc
     5641 tgccgcggat tcaacgcgaa cgcgttccga aaggcgattc accgcagcga agagctcgcc
     5701 gcgcagaaca tcgaaacaga tttcatcgtg ctcggaaaga agactgtgga tttcttccgt
     5761 cgacgccgtc taccggtggt cgaggcattc cccggcatct tccagcacct cgattttgca
     5821 acggcgacag cggtcgcgcg tctggcgacc gagcgtttcc tcaatggcga gactgatctc
     5881 gtcgagatag tctacaatga gttcgtttcg gtgctccggc agaacctcgt cacccggcag
     5941 tacctgccgt tgaaggcgac gcctgacgaa agcgaagaga gcgccaacga atacgacgaa
     6001 tacatctacg aaccggatca ggaatacatc tggcgcgcgt tactcgataa gtatctcaac
     6061 ttcgccgtgt ggcggatgct gctcgagtcg aacgcggcgg aacaggccgc gcgtatgacc
     6121 gcgatggaag aggcgacgaa caacgcggaa gagatgatca agaacctcat ctcggttcgc
     6181 aacaaggttc gtcagacgag cattacaacg gaactgactg agattgtcgg ctcggcggaa
     6241 tcactacgag cggattaaag acgcagatca ctccctacag gattgtgatg agatgagcga
     6301 agacaagaac attggacgca tc
//
LOCUS       DSCC01000031            2338 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031113, whole genome shotgun sequence.
ACCESSION   DSCC01000031 DSCC01000000
VERSION     DSCC01000031.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031113"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(79..549)
                     /locus_tag="ENN56_00315"
     CDS             complement(79..549)
                     /locus_tag="ENN56_00315"
                     /inference="COORDINATES: protein motif:HMM:PF01242.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HDS73960.1"
                     /translation="MSADTYRRHRFDRRRWRDAVVKITARYTFDAAHRLYDSALGSEE
                     NDRLYGPCSRIHGHTYRLEVSLRGDSLVHGMLIDFGRVDAVVNERIIDRLDHRVINEV
                     AELTGNTTTAEDIAQWIWSELDSAFTEDDVTLYEVTLYEGDRFSATVSRDDFRD"
     gene            complement(486..1475)
                     /locus_tag="ENN56_00320"
     CDS             complement(486..1475)
                     /locus_tag="ENN56_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73961.1"
                     /translation="MTSRERFLRTMAGEPVDRPPLWSEGIREDVRAEWAKAGYPLDRD
                     LDADFSIEPREQIQPETLHRDFRMVDRDSDSEELDLAAADPARYPDDWDDLIRKCRDR
                     DFTAGLRVSRGIFQILGVGSWASLSPLLIAIADEPDRVRKVMDDWARFALRVLERAFD
                     EIDYDYVLFSEPIASNSAPVIGPRAFARTCGEAYTRILRYARRRGVRWAVFQSYGHLT
                     PLIGEAMRMGMDAYWGGDVAPARTPYPELRRKFGRELALIGGIDAGLLEEDTRTLETE
                     IRRLVTPLVADGRYIPLLDGRVRSYVSFERYRAYRRTLIDVIDSIADDGGTRL"
     gene            complement(1506..>2338)
                     /locus_tag="ENN56_00325"
     CDS             complement(1506..>2338)
                     /locus_tag="ENN56_00325"
                     /inference="COORDINATES: protein motif:HMM:PF02894.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS73962.1"
                     /translation="DDLDALLARDDIDAVIVATPGFAHTDAVERAFAAGKHVFVEKPM
                     ALTPDECDRMIRARDAAGKKLMVGQVLRYIGVFNYTRELHAAGKLGDVIAVRTTRTSS
                     GWGGAGRPWRNREDLSGGVLFEFSVHELDFMMSLVGDAESVYAISHTTENAEWDYPDL
                     YMLNIGFQSGAMGQLAAGIADPVGHYGGEIIGTKGAVHFDARNGSLVARIDGEEAKTL
                     SYADIEPTEEPVRREVREFIEAVLNDTQVTIPGEEGRRVVRLAYAAKQSAREGRIVSL
                     "
BASE COUNT          480 a          760 c          645 g          453 t
ORIGIN      
        1 gtactacgcg gtatctcaac actgtatggt acaccgaatc aatcggcgaa caaacacacc
       61 cagccctttt tcttcgcctt aatcgcggaa atcgtcgcgg gacactgtcg cgctgaaccg
      121 atccccttcg taaagtgtaa cctcgtaaag tgttacgtcg tcttcggtaa acgcactgtc
      181 gagctcggac caaatccact gcgcgatatc ttccgcagtc gttgtgtttc cagtgagttc
      241 cgcgacctcg ttgatcacgc gatgatcgag tcggtcgata atacgctcat ttaccacggc
      301 gtcaacccga ccgaagtcga tgagcatacc gtgaacgaga ctgtcaccgc ggagtgatac
      361 ctccaaccga tacgtgtggc cgtgaatccg cgagcacgga ccgtacagcc gatcgttctc
      421 ctcactaccg agcgccgagt cgtagagacg atgcgccgcg tcgaacgtgt agcgcgccgt
      481 gattttcaca accgcgtccc tccatcgtcg gcgatcgaat cgatgacgtc gataagtgtc
      541 cgccgatatg cacgataccg ctcaaacgat acgtatgacc gaacgcgacc gtccaacagc
      601 ggaatatacc gcccatcagc aactaatggt gtaacgagac gacggatttc cgtttcgagg
      661 gtacgagtgt cctcctcgag cagacccgcg tcaatccccc cgatgagcgc gagttcgcga
      721 ccgaatttcc gacgcagctc cggatacggc gtacgcgccg gcgcgacgtc tccaccccaa
      781 tacgcgtcca tgcccatgcg catcgcctcg ccaatgagcg gcgtgagatg accgtagctc
      841 tggaacaccg cccaacggac gccacgccgc cgtgcgtagc ggaggatacg ggtgtatgcc
      901 tcgccgcacg tccgcgcgaa tgcccgcggt ccgatcaccg gtgcggaatt ggacgcgatc
      961 ggttcgctga acagcacgta gtcgtaatcg atctcgtcga acgcgcgttc aagcacccga
     1021 agcgcgaacc gtgcccaatc gtccatgacc ttgcgtacac gatccggttc gtccgcgatt
     1081 gcgatgagta acggcgaaag cgatgcccac gaaccgacgc cgagtatctg gaagatgcca
     1141 cgcgatacgc gcaatccggc ggtaaaatca cggtcgcgac atttccggat gaggtcatcc
     1201 cagtcatctg gataccgtgc aggatccgcg gcggcgaggt caagttcctc actgtcggaa
     1261 tcacgatcga ccatgcggaa gtcgcggtgg agggtctccg gttgaatctg ttcacgcggc
     1321 tcgatgctga aatcggcgtc gaggtcacga tcgagcggat agccggcttt cgcccactca
     1381 gcgcggacgt cctcccggat gccctcgctc cacagcggcg gacggtcaac cggttcgccc
     1441 gccatggtgc gaagaaaccg ttctcgggag gtcacactac gcctcctcag ccagtttgta
     1501 aaacgtcaga gcgatacgat acgaccttcg cgcgcgctct gtttcgccgc gtacgcgagt
     1561 cgaacgacgc gacgcccttc ttcacccggt atcgtcacct gcgtgtcgtt gagcaccgct
     1621 tcgataaact cgcgcacctc acgacgcacc ggctcttcgg tcggttcgat atcggcatac
     1681 gagagcgtct tcgcttcttc tccgtcgatt cgcgccacaa gcgaaccgtt tcgcgcgtcg
     1741 aaatgtaccg caccttttgt cccaatgatc tcaccgccgt aatgcccgac cggatcagcg
     1801 atccccgccg caagctgccc catcgcgccg gactggaagc cgatgtttag catgtagagg
     1861 tcggggtagt cccactcggc gttctcagtg gtgtgcgaaa tggcgtatac cgattccgcg
     1921 tcgccaacga ggctcatcat gaagtcgagc tcgtgcacgc tgaactcgaa aaggacgccg
     1981 ccgctcaagt cttcacgatt ccgccacgga cgacccgcgc caccccatcc tgacgatgtc
     2041 cgcgtggtcc gaacggcgat gacgtcgcca agcttcccgg ccgcatgaag ttcgcgcgta
     2101 tagttgaaca caccgatgta tcggagcacc tgaccgacca tgagcttttt cccggcggca
     2161 tcgcgcgcac gaatcatccg atcgcattca tcgggtgtga gcgccatcgg tttctcgacg
     2221 aacacgtgct ttcccgccgc gaaagcgcgt tcgacggcgt cagtgtgcgc gaacccggga
     2281 gtcgctacga tcaccgcatc gatatcgtcg cgcgcgagca gcgcatcgag gtcgtcta
//
LOCUS       DSCC01000032            1587 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052759, whole genome shotgun sequence.
ACCESSION   DSCC01000032 DSCC01000000
VERSION     DSCC01000032.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052759"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            99..>1587
                     /locus_tag="ENN56_00330"
     CDS             99..>1587
                     /locus_tag="ENN56_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73963.1"
                     /translation="MIHASINRLTDPLRATPDRVFIISFGILLLSAATSFLVHEYGVA
                     HHFGFPLDTGWLDIVTGRHVLRGEAVTSGSESPAYALILGVIDLLGHGNATFVAIVAK
                     TLSLAAFIVTGWACFRLVQRFLGDGTLGVLTVVILVSLSPALVWSGTSGLGIAWGTAM
                     IALGFRDFADNRHVAGTAWLGVACWFTPIALPILLMSYSVRLRTGTLLRIAIGAAAIS
                     GWIAWRLVSGTSIMVPLETSDWLTWGANWLSLVGAARVGYVHPPLLALLVAIGVFRLR
                     RQGLALIAIVVVPAVVVPIIEPNPGAFGRLIFPILPVVFGLATAGIDLIATRQSHGLL
                     RGRRLAIALLAMYIVWVGPTLWMVRTIFAWQVENTVRAGVDVGRWIGENANHGELIAT
                     TAPGAVGYFSQHVVIDLTRVRDIDEAIMITRPPWVAVNVSLSIPDAIREDYEAVHTTA
                     FRVRTSVYPPGPLVVFRRMYRAGMATTTPQPTFTHDGPSAGKEAYL"
BASE COUNT          274 a          468 c          479 g          366 t
ORIGIN      
        1 gggtatccat gacgcgctac ccgaaaaacg cgacgggacg acgtggaacg tgacgctcct
       61 tagcgtcgga tatcactggt agacggacac tccgcccgat gatacacgca tccatcaacc
      121 gccttaccga tcctctacgt gcgactcccg accgcgtgtt catcatctca ttcggcatct
      181 tgcttctcag tgcggcgacg tcgttcctcg tccacgaata tggcgtcgcg caccatttcg
      241 ggttcccgct cgataccggt tggctggata tcgttaccgg ccggcacgta ctccgtggcg
      301 aggcggtaac gtctggctcc gaaagtcccg cgtatgcgct gatactcggt gtcatcgatc
      361 ttctcggtca cggaaacgcc acgttcgtcg cgatcgtcgc gaaaacactg agccttgcag
      421 cgttcatcgt taccggctgg gcgtgctttc gactggttca acggttcctc ggcgacggaa
      481 cactcggcgt tctcaccgtc gtgatactcg tttcgctttc gcccgcgctt gtctggagcg
      541 gaacatccgg tctcggcatc gcatggggaa ccgcgatgat cgcgctcgga ttccgtgact
      601 tcgccgacaa ccgacacgtc gccggcacgg cgtggctggg tgtcgcgtgc tggtttacgc
      661 cgattgcttt accaatactc ctcatgtcgt acagtgtccg tctgcgtacg ggaaccctcc
      721 tccgaatcgc cattggtgct gcggcgatta gtggatggat cgcgtggcgg ctcgtcagcg
      781 ggacctcgat tatggtgcca ctcgagacgt cggattggtt gacttggggc gcgaactggc
      841 tctcgctcgt tggtgccgcg cgggtcggat acgtgcatcc gccgttgctc gcactcctcg
      901 tcgctatcgg tgtgtttcga cttcggcgac agggattggc gctgatagcg attgtcgttg
      961 taccggcagt ggttgtgccg attattgagc cgaatcccgg cgcgttcggg cgattgatct
     1021 tcccgatcct tccggtggta ttcggtcttg cgacagccgg tatcgacctg atcgcgacac
     1081 ggcaatcgca tggactcctt cgtggacgga ggctcgcgat cgcgctgctc gccatgtata
     1141 tcgtgtgggt cgggccgaca ctctggatgg tgcgtacaat cttcgcatgg caagtggaaa
     1201 acaccgtgcg agcaggcgtc gatgtcggtc ggtggattgg cgaaaatgca aatcatgggg
     1261 aactcatcgc gacgacggca ccgggagccg tggggtattt ttcgcaacac gtcgtcattg
     1321 acctgacccg ggtgcgcgat atcgacgagg cgatcatgat cacgcgtcca ccgtgggttg
     1381 cggtaaacgt ttccctatcg attcccgacg cgattcgaga agattacgaa gcggtacata
     1441 ctacggcatt ccgtgtgcgt accagtgtct atccgcccgg accgctcgtg gtgtttcgac
     1501 ggatgtaccg agcgggcatg gcgacgacta cgccgcaacc cacatttaca cacgatggtc
     1561 cgtctgccgg gaaggaagca tatttac
//
LOCUS       DSCC01000033            2073 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036687, whole genome shotgun sequence.
ACCESSION   DSCC01000033 DSCC01000000
VERSION     DSCC01000033.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036687"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="ENN56_00335"
     CDS             complement(<1..1431)
                     /locus_tag="ENN56_00335"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS73964.1"
                     /translation="MHAIRRRTTDSSSLPARSCSLARRWRHSVSLRHSQRYIIPPEGS
                     ETFTIRVALPVRMVSQQSRCAPEGHSGRCARQNETKERTMAEKLAIDGGTPIRTKPMP
                     PGNPGEVLIGVEEREQVLEVLDAHSPFRFYGPNVQKKAEQFESEFTSFVGMKNALGVT
                     SGTAALKVSLLAAGVGPGDEVIVPAVTFIASAGVVVACHARPVFVDVGPDMGIDPDDL
                     ERHITERTKAIMPVHILGGAANMEPIMRVAEKHGITVIEDCAQSAGAEYHGKRIGSFG
                     HINAFSLQFQKVITAGEGGVVTTNDGRLFDRARRAHDHGLNRWDAGKPEDAFCGEVYR
                     MNEMSGAFALAQLRKLPRMLETMRKHSKRVRDAVAKYDDLELRSLADADGATGQYTMF
                     YARTKELADRWIQALQKEGVGGMRLYGGTLVLEHPQIMNQRTASEHGPFDSPLYPERI
                     VYRVEDYRQSKALLDRSVWILTSPAYT"
     gene            complement(1331..>2073)
                     /locus_tag="ENN56_00340"
     CDS             complement(1331..>2073)
                     /locus_tag="ENN56_00340"
                     /inference="COORDINATES: protein motif:HMM:PF02875.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73965.1"
                     /translation="VMKSGVPVLIQRQLPEVESVLLEHAAEVGAIPYRIDDAMSITDS
                     GDGAAWTLRVTAPSNSPVVSYEKLTFGALGDHQRRNFAAAVVVLHLAGVPLDPDRVRT
                     VAATTTIPGRLQLVPETPPILLDVAHTPDSARMLVDVIRNRFPGRRTTLVFSCAQDKR
                     VAEIAEILASVAERVVLVPIPGIRAMPMEDMAVAWKAVHPFVTTSTSTRDAIENARDT
                     TPNHGLIVVTGSFVLIGAAMEALGITPT"
BASE COUNT          395 a          658 c          605 g          415 t
ORIGIN      
        1 ggtgtatgcc ggcgacgtga ggatccacac cgagcgatcg aggagcgctt tcgactgccg
       61 gtaatcctcg acgcggtaca caattcgctc cggatacaac ggcgaatcga acggaccgtg
      121 ctcgcttgcc gtccgctggt tcatgatctg cggatgctcg agcacgagcg tgccgccata
      181 cagccgcatc ccaccgacgc cttccttctg caaggcttga atccagcggt ctgcgagttc
      241 cttcgtgcgg gcgtagaaca tcgtatactg gccagtcgca ccgtcggcgt cggcaagcga
      301 acggagctcg aggtcgtcat atttggcaac ggcgtcgcga acgcgcttgc tgtgcttgcg
      361 catcgtttcg agcatccgcg gcagtttccg caattgcgca agggcaaacg cgccgctcat
      421 ctcgttcatg cggtacactt cgccgcagaa cgcgtcttcg ggttttccgg cgtcccaacg
      481 gttgagcccg tgatcgtgag cgcgacgggc gcggtcgaag agacgaccgt cattcgtcgt
      541 aacgacgccg ccctcacccg ccgtaatgac cttctggaac tgcaagctga atgcgttgat
      601 atggccaaat gagccgattc tcttgccgtg atactccgca ccggccgatt gcgcgcaatc
      661 ttcgatcacg gtaataccgt gcttttccgc gacccgcatg atcggctcca tattcgccgc
      721 gccgccgagg atgtgcaccg gcatgattgc tttcgtccgc tcggtgatgt gccgctcgag
      781 gtcgtcaggg tcgatgccca tatcgggacc cacatccaca aataccggtc gcgcgtgaca
      841 cgcaacgacg acacccgcgc tcgcgataaa cgtcactgcg ggaacaatca cctcgtcgcc
      901 cgggccgact ccggcggcga gcaagctgac cttcagcgcc gcagtgcccg acgtaacgcc
      961 aagggcgttc ttcattccga cgaagctcgt aaactcggac tcgaattgct ccgccttctt
     1021 ctgcacattc gggccgtaga aacggaacgg cgagtgcgcg tcgagcacct cgagcacctg
     1081 ttcgcgctct tcaacaccga tgagcacctc gcccgggttt cccggaggca ttggtttcgt
     1141 gcggattggt gtgccgccat cgatcgcaag cttctctgcc atggttctct ctttcgtttc
     1201 gttttgccgc gcgcaccgac cggaatgacc ttccggagcg caacgcgatt gttgggatac
     1261 catcctaaca ggtaaagcta cccgaattgt gaatgtttcc gaaccctcgg ggggtatgat
     1321 gtagcgttgg ctatgtcgga gtgataccga gtgcctccat cgccgcgcca atgagcacga
     1381 acgagccggt aacgacgatg agtccgtggt tcggcgtcgt atcgcgtgca ttctcaatcg
     1441 cgtcgcgcgt cgacgtggac gtcgtgacga agggatgcac cgctttccat gcgactgcca
     1501 tgtcttccat cggcatcgcg cggattcccg gaatcggcac gagcacgacc cgctcggcga
     1561 ccgatgcgag gatttccgcg atctccgcga cgcgtttatc ctgtgcgcaa ctgaacacaa
     1621 gcgtcgtgcg gcgtccggga aagcgattgc gaatcacatc gacgagcatt cgcgccgaat
     1681 ccggcgtatg cgcgacatca agcaggatcg gcggtgtttc gggaacgagc tgcaaacgtc
     1741 cggggatcgt cgttgttgcg gcgacagtgc gcacgcgatc cggatcgagt ggaacgcccg
     1801 caagatgcag cacgacgacg gctgcggcga aattccgccg ttggtgatca cccaacgcgc
     1861 cgaacgtgag cttctcatac gatacgacgg gtgaattcga cggcgcagtc acccgcagtg
     1921 tccacgccgc cccatctccg ctatcggtga tcgacatcgc gtcgtctatc cgatagggaa
     1981 tcgcaccgac ttccgccgca tgttcgagaa gtaccgattc gacctcggga agctgccgct
     2041 gaataagcac cggcacgccc gacttcataa cac
//
LOCUS       DSCC01000034            2430 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029569, whole genome shotgun sequence.
ACCESSION   DSCC01000034 DSCC01000000
VERSION     DSCC01000034.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029569"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(82..1596)
                     /locus_tag="ENN56_00345"
     CDS             complement(82..1596)
                     /locus_tag="ENN56_00345"
                     /inference="COORDINATES: protein motif:HMM:PF14667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDS73966.1"
                     /translation="MSATAVRGDAETLARGAATNLLGNLGSLSQALAMFVIARAFGAQ
                     VLGLYALAWMLVRIIARLVGLGLDPAIVYHLNRVTADDEQDREGQLVGTSSLLVVIAS
                     VISGVILWLGMDLVAYGIFDEPRLVIAYRAFVFALPAMGLTGVLLAATRARKVMKYQV
                     IAMSFVQPFSFLMGSLVAWRIDGGLAALAIAQTAAVWITTGVSVLFYGTIFRIGPLIR
                     GFFRPTEVAGLLRYGLPLAGKGMLALAVTRTDLFLVGHFLGAAAAGIYSLAADVAQLI
                     RMARMALEPILGPLVAEQHHRADRDRLSATYGRATRWALIVNLALSGVVAIAGETILS
                     LYGKTFTAGAFALAILLLGQVIAGAFGLSEMMLTMIGHPTLVFGNMALLLGLIGVLDY
                     AFIQLWGLAGAAIGTASATILVTLVQVRQVRKRTGMHPWRFAILRPAVAFICAVTPAL
                     FVPLPTMHTAVDQIIRSVAFLLLYVVFLSRFGLETEDRHYVGTAIHRFRARRNR"
     gene            1835..>2430
                     /locus_tag="ENN56_00350"
     CDS             1835..>2430
                     /locus_tag="ENN56_00350"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73967.1"
                     /translation="MQHRNIIVFMMDALRPDHLGPWSVNNRATPNFSRLADEGVFLRE
                     FFGHMPSSYNARASVLTGRDPHTHGVKINGRPLPSKEVTLPQILRDAGWLTALTTPYP
                     KGMNRGFSENTLRKPFENNPRAVIEALPGIGEGIARTDMAQDTAALVTWLRQWAADPQ
                     RRRKPFFLWADEEFSHGPWRPPAPYDSMYDHESDEPPYT"
BASE COUNT          600 a          757 c          648 g          425 t
ORIGIN      
        1 gccaacaacc ttccgatatg gtcgcataca aagataccgt atctcaatta ttccatgaaa
       61 catacgaacg cgtttccgac atcaacggtt ccgtcgcgca cgaaaacgat gtatcgccgt
      121 acctacatag tgccgatctt cggtctctaa tccgaagcga ctcaagaaaa ccacgtaaag
      181 cagaagaaac gcgacggatc gaatgatctg gtcaaccgcc gtgtgcatcg tcggtagtgg
      241 aacgaacaac gccggtgtca cggcacatat gaacgccacc gccggacgga gtatcgcgaa
      301 ccgccatggg tgcatacccg ttcgcttgcg gacttgtcga acctggacga gtgtaacaag
      361 aatcgtcgcg gacgccgtcc caatcgccgc tccggccagc ccccaaagct ggatgaacgc
      421 gtagtcgaga acaccaatca agccaaggag cagtgccatg ttcccgaata cgagcgtcgg
      481 gtgtccgatc atcgtcaaca tcatttccga cagcccgaac gcaccggcaa tgacttggcc
      541 aagaagcaga atcgcgagtg cgaacgcacc cgcggtaaac gtcttcccat ataaactgag
      601 aatcgtttcg cctgctattg caacaacccc cgaaagcgca aggttcacaa tgagtgccca
      661 acgcgtagcg cgaccgtatg tcgcactcag tcggtcgcga tctgcgcgat gatgttgttc
      721 agcgacgagt ggtccgagta tcggttcaag tgccatacgt gccatgcgaa tgagctgtgc
      781 gacatccgct gcgaggctat agatgcccgc tgcggcagcg ccgagaaagt gtccgacgag
      841 aaaaagatca gttcgcgtaa ctgcaagagc gagcatgccc ttacctgcca ggggaagacc
      901 atatcggaga agcccggcga cctctgtcgg gcgaaagaac ccgcgaatga gcggacctat
      961 ccggaagatc gtcccgtaga acagcacgct cacgccggtg gtgatccaga cggcagccgt
     1021 ctgcgcaatc gcgagcgcgg caagaccacc gtcgatccgc cacgcaacga gactccccat
     1081 gagaaacgaa aacggctgaa cgaagctcat ggcgatgacc tgatacttca tcaccttccg
     1141 cgcgcgcgta gccgcgagca gtacgccggt gagccccatc gcaggcagtg cgaacacaaa
     1201 ggcacgatac gcgattacca accgcggctc atcgaagatc ccgtacgcaa cgaggtccat
     1261 accgagccag agtatcaccc ctgatatgac cgaggcaatc acgacaagga gcgaactcgt
     1321 gccgacgagt tgaccttcgc gatcttgctc atcgtccgct gtcacccggt tgaggtgata
     1381 gacaatcgcg ggatcgagcc caaggcctac aagccgcgcg ataatacgca ccaacatcca
     1441 cgcaagcgcg tagagcccga gcacctgcgc gccgaacgca cgtgcgatga cgaacatcgc
     1501 cagcgcttgc gacaggctgc ccagattccc gagcagattc gtcgcagcgc cccgcgcaag
     1561 tgtctcagca tcaccgcgaa ccgcggtcgc actcatcgta ctctcatccg atcggttgac
     1621 atgctcggtt atgacactgg tggcgttcga cgatacgcat attcgattcc aaccgacacc
     1681 agcatgataa gatacgataa tgacgtgaaa atcactgaat agcgtgcggc atcaccccga
     1741 aagacgcaat gtctgcaacc ttcccccgtc tattcgtgga gactatgctt ctgtcaatca
     1801 atcccacgca cgcatccgca ctttggagta tacaatgcaa caccgaaaca ttatcgtgtt
     1861 tatgatggac gcgttacgcc ctgatcacct cggaccgtgg agcgtcaaca accgcgcgac
     1921 accgaacttc agccgtctcg ccgacgaagg cgtcttcctc cgcgagttct tcggtcacat
     1981 gccgtcgagc tacaacgctc gtgcgagcgt cctcaccggg cgcgatccgc atacccacgg
     2041 agtgaagatc aacggacgac cgcttccgtc aaaggaggta acgcttccgc aaatccttcg
     2101 tgacgccggg tggctcactg cgttgacgac gccgtacccg aaagggatga atcgggggtt
     2161 ttccgaaaac acactgcgaa aaccattcga aaacaaccct cgcgcggtca ttgaggcact
     2221 tccgggcatc ggtgagggaa tcgcacgcac cgacatggca caggatacgg ctgctctcgt
     2281 aacgtggcta cgccagtggg cggctgatcc gcagcgccga cgcaagccgt ttttcctctg
     2341 ggccgatgaa gaattcagcc acggtccgtg gcgtccgccc gcgccgtatg actcgatgta
     2401 cgatcacgaa tcggacgagc cgccgtatac
//
LOCUS       DSCC01000035            1693 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048270, whole genome shotgun sequence.
ACCESSION   DSCC01000035 DSCC01000000
VERSION     DSCC01000035.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048270"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..268)
                     /locus_tag="ENN56_00355"
     CDS             complement(<1..268)
                     /locus_tag="ENN56_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73968.1"
                     /translation="MLDYYQEDCFQNGGCFVRWEYVVSPEDIVGQAHASTIVETSKGD
                     LLCAWFGGAREGDKAVAIWMSRRTEDGWGAAQIVVDEPNVPTWNP"
     gene            complement(272..>1693)
                     /locus_tag="ENN56_00360"
     CDS             complement(272..>1693)
                     /locus_tag="ENN56_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS73969.1"
                     /translation="ETNRRQYEWPDVWESLVFATPDEGLYWPESGIRGRGRALFASPV
                     RDETGDVLGNFRRDLDISGLSSTIRGWNAREQRWMGVDELERLSIGDAITFAWDEAAP
                     VALVGPGETVQLDSAQVIELEPGWNLIGQPYPYPISWDAVVEANAELTVSRPWVIDDG
                     LRIASIWKPWYGAWVYLDDEQAATLTIPPRDTVLVDESLDTTATRRAPIRLDRSEGWL
                     SDESFLLSLSARSASCRINDIAIGWNTHADDAWDAIDVPIPPSPDSSLVIRIQHDDWE
                     RRSGDYAVDVRTPGTGGIWRFLVDVNDNGPVELRLSFADPLPTGIYADLIDLMVLDSV
                     RLDQQSVTYQLRDLARFPQREIAVVVGDERFRDEVEREEMLAPSRLTLYQNYPNPFNS
                     ETTITYSIPGHEGVGMRAQLVVYNMLGQQVRLLHDGPSNPGIHTFSWDGRDNQGMSVS
                     SGVYFCELVWGSERKLQRLIYLR"
BASE COUNT          391 a          529 c          413 g          360 t
ORIGIN      
        1 ccggattcca cgttggcaca ttaggttcat caacaacaat ttgcgctgcg ccccatccat
       61 cttcagtgcg acgcgacatc cagatcgcaa ctgctttatc accttctcgc gccccgccaa
      121 accaagcgca aaggagatca ccttttgacg tttctacgat cgtcgatgcg tgcgcttgac
      181 cgacgatgtc ctccggactc acaacgtatt cccaacgcac gaagcatcct ccgttttgaa
      241 agcagtcctc ttggtaatag tccaacagtg tctaccgtaa gtatattagg cgttgaagtt
      301 tacgctccga accccacacc aactcgcaga agtacactcc cgaactcacc gacatcccct
      361 gattatcgcg gccgtcccac gagaacgtgt gaatccccgg attcgacggt ccgtcatgca
      421 gcaatcggac ttgctgtccg agcatgttat atacgacgag ttgcgctcgc attccgacac
      481 cttcgtgtcc gggaatagaa tacgtgattg tcgtttcgga attgaagggg ttcggatagt
      541 tttgatagag cgtgagccta ctcggtgcaa gcatttcttc gcgctcaacc tcgtcgcgga
      601 accgctcgtc gccaacaaca accgcaatct cacgttgcgg gaaccgtgcg aggtcgcgaa
      661 gttggtacgt taccgactgc tgatcaagac gtacgctgtc aagtaccatg aggtcgataa
      721 gatccgcgta aatacccgtc ggcaacggat ccgcaaacga gagccgcagc tcgaccggtc
      781 cattgtcatt gacatccacg aggaagcgcc aaatcccacc ggttcccggc gttcgcacgt
      841 cgaccgcata gtcaccggag cgacgttccc aatcatcgtg ttgaatacgg atgacaagac
      901 tgctatccgg tgacggcggt atcggcacgt cgatggcgtc ccacgcatcg tctgcatggg
      961 tattccatcc gatcgcaata tcgtttatac gacacgaagc actccgagcc gaaagcgaca
     1021 aaaggaacga ttcgtcgctc aaccatccct cgcttcgatc aagccgaata ggtgcgcgac
     1081 gagtcgccgt agtgtcgaga ctctcgtcaa ccaacacggt atcacgtggc ggaattgtca
     1141 gcgtagcagc ctgttcatcg tcaagataca cccacgcgcc ataccagggt ttccagatac
     1201 ttgcgatccg caatccgtcg tctattaccc acggccttga tacagtgagt tccgcattcg
     1261 cttcgacaac cgcgtcccac gaaatcggat acgggtatgg ctgtccgatg agattccagc
     1321 ccggttcgag ctcgataacc tgcgcggaat cgagttgaac ggtttcaccc ggtccgacta
     1381 acgcgacagg agcagcttca tcccacgcga acgtaatagc gtccccgatc gataggcgct
     1441 ccagttcatc gacacccatc caacgttgct cacgggcgtt ccacccacga atcgtcgatg
     1501 agagcccgct tatgtcgaga tcacgccgaa aatttcccag aacgtcgccg gtttcgtcgc
     1561 gaactggtga cgcgaagaga gctcgaccac gcccccgtat gcccgactct ggccaataca
     1621 gcccttcgtc cggtgtcgca aacaccagtg attcccacac gtcgggccat tcatactggc
     1681 gacgattcgt ctc
//
LOCUS       DSCC01000036            5443 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1009940, whole genome shotgun sequence.
ACCESSION   DSCC01000036 DSCC01000000
VERSION     DSCC01000036.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1009940"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..566
                     /locus_tag="ENN56_00365"
     CDS             <1..566
                     /locus_tag="ENN56_00365"
                     /inference="COORDINATES: protein motif:HMM:PF02502.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDS73970.1"
                     /translation="PLAKDALAATGIVLASALPSRETTAHTAHGIQQQQSGRKDLDRV
                     AVGVVPSGASLESILFATLRRSNRIPLRVPTSRREAAHLARQVAGAVSGGQAAWGVII
                     DESGIIGAAVANRVTGVIAALCNDETTARRARGQVGANVLCVAADIVAGPLFERILSA
                     WIETPAQCDEDTANVIRELDRCQESAR"
     gene            563..1303
                     /gene="deoC"
                     /locus_tag="ENN56_00370"
     CDS             563..1303
                     /gene="deoC"
                     /locus_tag="ENN56_00370"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDS73971.1"
                     /translation="MSKVDAATVERIIIEVLRRLEPGNTPIENLSSYIEHTLLRPDTV
                     PEQIDHLCVEAKKHIFYAVCVPPRYVQHAKDHLNGSAVKVVSVVGFPHGAALTSTKRA
                     EAESLVEIGVDEVDMVLPIGALKAGDWRAVAADISAVVGVAHRNDAPQPLVKVILETA
                     LLTDREIVAACIIAQDCGADFVKTSTGFNGSGATVDHVVLMRQTIGESMGIKAAGGIR
                     LREHAESLIRAGATRIGTSSGPALLTPN"
     gene            1417..2772
                     /locus_tag="ENN56_00375"
     CDS             1417..2772
                     /locus_tag="ENN56_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73972.1"
                     /translation="MKNRISLGWAERELPESVNQDLTGFLDRPNPCEGKRDPLYARVL
                     YLNDGRNHAVYVSVDMLGMGQALTERIRAASSHEHNIPPRAYTFAATHTHSAPPAVFL
                     ANRGVVDRDWNRELEQVILDVITDAVMGRTKRVRLRFAQTDAPGFTYSRRTHDSDGTP
                     RKGKRSAKAGASPDSEIDTTLSVIVANDLRTGNPVAAMVHFPANPVFYRDNLSSADYP
                     GRLVQKVNESVGRGFRSMFIQGPCGNINPLRLSANVDEDVERMSAGLAEKAIELIRDK
                     NAGTYLVPKINARLRYDTLPWVTTDVDEDALEKLVLSPEDWTKADLNTQADHVWAKRV
                     LVTRSRGWWIDGVRVPIGVTMFGELILVAAPGEIFSETIERLRAAHPDRMFIGVGHAN
                     DDVGYIPSTDAYTKGGLGLETMHRVYGYSNPVSPKAEEVILTTVEDVLNVGTMAIPEV
                     K"
     gene            complement(2862..3743)
                     /locus_tag="ENN56_00380"
     CDS             complement(2862..3743)
                     /locus_tag="ENN56_00380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDS73973.1"
                     /translation="MLALRGSYVALVTPFTQDDRVNHALLRDLVEWHIEQGTSGLVPV
                     GTTGESPTLTYEEHEAVIETVVKAVDGRIPVLAGTGSNSTREAVHMTHHAKMVGADGA
                     LIVLPYYNRPSQAGLIAHITTLNDVGLPLVIYNIPSRTGINMEPPTFEKLSHLENVVG
                     VKEASGNINQMSEVVLVTAGRCAVMSGDDALTLPLLAVGGTGVISVIANIVPNEMASL
                     VSAWERGDRAEALRLHQRLFRIAKATLSLETNPGPIKEAMAMLGHDVGAVRPPLAPLS
                     AENREKLRAVLVDMELL"
     gene            complement(4012..4194)
                     /locus_tag="ENN56_00385"
     CDS             complement(4012..4194)
                     /locus_tag="ENN56_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73974.1"
                     /translation="MSVNGMLNTERVMKKPGMKPLTGKNAASRRKIRRVTSKARRRIS
                     RSVVLRELEWDLANAL"
     gene            complement(4389..5441)
                     /locus_tag="ENN56_00390"
     CDS             complement(4389..5441)
                     /locus_tag="ENN56_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDS73975.1"
                     /translation="MHMGNLSRVSKAKSRSISAENFTGAKGEGGKATEGLGFNQSREL
                     GKGWKISPAVRIEKGSTFTIADIEGPGAIQQIWMTPTGHWRHSILRIYWDGQKQPSVE
                     CPVGDFFANAWGPYAQVSSLAVCVNPKSGLNCYWEMPFRKHCRITMENIGLDDMTLFY
                     QVNYTLTDVPDDAAYFHAQFRRTNPLPYKEVYTIADGIAGWGQYVGTYMAWGVNNNGW
                     WGEGEIKFYLDGDTDYATIVGTGTEDYFCGSYNFDIGGKYQEFTTPYAGMPQVIRPDG
                     LYQSQQRFGLYRWHIMDPIRFEKDLRVTIQSLGWKSQGRYNPQQDDMASVAYWYQTLP
                     TARFPELPDRNYLEII"
BASE COUNT         1150 a         1554 c         1599 g         1140 t
ORIGIN      
        1 cgccgcttgc gaaggacgct ctcgccgcaa cgggtatcgt tctggcaagc gccctgcctt
       61 cgcgcgagac aacggcgcac actgcgcacg gcatacaaca gcaacaatcc ggtcgcaaag
      121 acttggaccg tgtcgcggtc ggggtggtac cttccggcgc gtcgctggag tccatacttt
      181 tcgcgacgct ccgtcggtcg aaccgtatcc cacttcgcgt cccgacatcg cggagagaag
      241 cggcgcacct cgcgcgacag gttgccggtg cggtctctgg cggccaagcc gcgtggggcg
      301 ttatcatcga tgagtcaggg atcatcgggg cagccgtggc gaatcgagtg accggcgtca
      361 tcgcggcact gtgcaacgat gaaacgacgg cgcgtagagc gcgtgggcaa gtcggtgcaa
      421 atgtcctgtg cgtcgcggcg gatatcgtgg cgggtccgct cttcgaacgc atcctcagtg
      481 cgtggatcga aactcccgca cagtgtgacg aggacactgc gaacgtcatt cgcgaacttg
      541 accggtgtca ggaatccgcg cgatgagcaa ggtcgacgcg gcaacggtag aacgcataat
      601 catcgaagtt ctcagacggc tggagccggg gaatactccg atagagaacc tgtcgtcata
      661 catagagcac acgcttcttc ggccggacac ggtaccggaa cagattgacc atttgtgcgt
      721 tgaagcaaag aagcacatat tttatgcagt atgcgtacct ccgcgatatg ttcaacatgc
      781 gaaagaccac ctgaacggct ccgccgtgaa ggttgtgtcg gtggttggtt ttccgcatgg
      841 tgcagcgttg acttctacga aacgagccga ggcggaatca ctcgtcgaga ttggtgtcga
      901 cgaggtggat atggttctgc cgatcggcgc actaaaagcc ggtgattggc gggccgtcgc
      961 tgcggatatt tcggcggttg ttggtgtagc ccacaggaat gatgcgccgc aaccgcttgt
     1021 caaggtcatt ctcgaaaccg ctctgctcac cgatcgggaa atcgtggccg cgtgcatcat
     1081 tgcgcaggat tgcggcgccg acttcgtcaa gacgtccacg ggtttcaacg gctcgggagc
     1141 aactgttgac cacgtcgtgc tgatgcggca gacgattggc gaatcgatgg gtatcaaagc
     1201 ggcgggcgga atacgtttac gggaacacgc cgaatcattg attcgtgccg gagcgacgcg
     1261 aattggaacc agttcggggc ctgcgttgct tactcccaat tgatatagcc gagacgtcgg
     1321 gtagtctgag tttgtcaacg accatgtcgt tcgctgatcg tgggggatcg acggacacag
     1381 aatcccgtgc agaatcgggg aaggaagata cgtgcaatga agaacagaat ctctctcgga
     1441 tgggcagaac gggagttacc agaaagtgta aatcaggatc tcactggttt tctggaccgc
     1501 ccaaacccgt gcgaaggtaa acgtgatcct ctctacgcac gcgtattgta tttgaatgac
     1561 ggccggaatc atgccgtgta cgtgagcgtc gacatgctgg gtatgggaca ggcgcttacc
     1621 gaacgaattc gcgcggcatc aagccacgag cacaacattc caccgcgcgc gtatacgttc
     1681 gccgcaacac acacgcacag cgcacctccc gcagtattcc tcgcgaatcg cggcgtcgtc
     1741 gaccgcgact ggaatcggga actcgagcag gtgattctcg acgtcatcac cgatgccgtc
     1801 atgggtcgga caaagcgcgt gcggctccgg ttcgcgcaaa cagacgcgcc cggattcacg
     1861 tacagtcgcc gtacccatga ttctgacggc actccccgga aagggaagcg tagcgccaag
     1921 gctggcgcga gtccggatag tgagatcgat acgacgctct cggtaatcgt tgcgaatgac
     1981 ctgcgtaccg gcaatcctgt cgccgcgatg gttcatttcc cggcgaatcc ggtgttctac
     2041 cgcgataacc tcagcagtgc cgattatccg ggcaggctcg tccagaaggt caacgagtcc
     2101 gttggccgcg gtttccggtc gatgttcatt cagggaccgt gcggcaatat caatccgctt
     2161 cggttgagcg cgaacgtaga cgaggatgtc gagcgaatga gcgccggtct cgctgaaaaa
     2221 gccatcgagc tgattcgcga caagaatgca ggcacgtacc ttgtgccgaa aattaacgcg
     2281 cggctccggt atgacacgct cccatgggtt acgacggacg tggacgagga cgcacttgag
     2341 aaactcgtac tttcaccgga ggattggacg aaagccgatc tgaacacgca ggccgatcac
     2401 gtttgggcga agcgcgtact ggtcacacgg agtcgtggct ggtggatcga cggcgttcgc
     2461 gtcccgattg gcgtgacgat gttcggcgag ttgatactcg ttgcagcgcc gggtgaaatc
     2521 ttctctgaga cgattgagcg tttgcgtgct gcgcatccgg accggatgtt tatcggcgtg
     2581 ggtcatgcga acgacgacgt tggctacatc ccgtcgacgg acgcgtacac gaaaggcgga
     2641 ctcgggctcg agaccatgca tcgagtctac ggatattcaa atccggtcag tccgaaggct
     2701 gaagaggtca tcctcacaac ggtagaagac gtgttgaacg tcggaacgat ggcgattccg
     2761 gaagtgaaat agccttcgcc ggcaacagaa acgagagcag gggaacgatc cgtaaccacc
     2821 ggatcgttcc cctgtttgtt tcgaacgtgg tgtgtattct ctcagagaag ttccatatcg
     2881 accaggaccg cacgcaactt ctcacggttc tccgcgctca atggtgcaag tggcggacga
     2941 accgcgccga catcgtggcc gagcattgcc atagcctcct tgatcggacc gggattcgtt
     3001 tcgagactca acgtcgcctt tgcgatgcgg aacaggcgct gatgcaaccg gagagcttcg
     3061 gcacggtcgc cgcgttccca tgcggatacg agcgacgcca tctcattcgg gacgatgttc
     3121 gcgatcaccg aaataactcc ggttcccccg acggcgagca agggcaacgt gagcgcgtcg
     3181 tcgcctgaca tcaccgcgca gcgtccggcg gtcaccagga cgacctcgct catttggttg
     3241 atgtttccgc tcgcttcttt cacaccgacg acgttttcga gatgagagag cttttcgaat
     3301 gtcggcggct ccatgttgat gccggtccga ctcgggatat tgtagatcac cagcggtagc
     3361 ccgacatcgt tcagggtcgt tatgtgcgcg atgagaccgg cttgcgacgg acggttgtag
     3421 tacggtagta cgatgagcgc gccgtcggca ccaaccatct tcgcgtgatg ggtcatgtga
     3481 acggcttcgc gcgttgagtt cgagccggtt ccggcgagaa ccggtatgcg cccgtcgacg
     3541 gccttaacca cggtctcgat gacggcttca tgttcttcgt aggtcagcgt tggagattca
     3601 ccagtcgtgc cgacgggtac caatccgctc gtcccctgtt cgatgtgcca ctcgacgaga
     3661 tcgcgcaaca acgcgtgatt gacacggtcg tcctgagtga acggggtgac aagagcgaca
     3721 tacgatccgc gaagtgcgag catgtgcggt tcctcattgc gtcgtgattc ggcagcgtag
     3781 gcgtacacca tcgctcgccc agcgctcacc gatcgacgat catgttctca gcagcggttt
     3841 tattggtaaa gacgggttcg acgtcatggc agtacggagc gcgtcgaaga atccggcaag
     3901 tgagtatagg agtcgaggcg agacgagtca acgcactgac gttaaacacg caagggtcgg
     3961 accggataac ccggtccgac ccctgtgccg tctatgaaat ggaacgcgac cttagagtgc
     4021 gttcgccaaa tcccactcga gttcacgaag cacaaccgat cgcgatatgc gccgccgcgc
     4081 tttcgatgta acgcgacgga ttttccgccg actcgcggcg ttttttccgg ttagtggctt
     4141 catacctggc ttcttcatga cacgttcggt gttgagcatc ccgttcaccg acatggaacc
     4201 ctcctgtcga agaaatatca gcaactccct ttacgtcgag gacaatcctc ggggagacgc
     4261 cgcggtcgca aaacgacttc cgcgcctgct cggaaagcta tcggtacacc tgtacgctgt
     4321 caatattccc ttctacaaaa tcgctataat ccccaccgaa tggcgcttaa gttcggagtt
     4381 caccctcgtc agatgatctc cagataattc cgatcgggaa gctccggaaa acgcgcggtc
     4441 ggaagcgtct gataccagta cgccactgac gccatgtcat cctgctgcgg attgtaccgg
     4501 ccctgcgact tccatccgag cgactgaatc gtcacacgca agtctttttc aaaccgaatc
     4561 ggatccataa tgtgccagcg atagagtccg aatcgttgct gcgactgata caggccgtcc
     4621 ggacggatca cctgcggcat acccgcgtac ggggtggtaa actcctgata cttcccgccg
     4681 atgtcgaagt tgtacgatcc gcagaagtag tcttcggtgc ccgtgccgac aatcgttgcg
     4741 tagtcggtgt cgccgtcgag atagaacttg atctcgcctt cgccccacca accgttgtta
     4801 ttcacgcccc acgccatata cgtgccgacg tactgacccc atccggcgat tccatcggcg
     4861 atggtgtaaa cctctttgta cggaagcgga ttcgtccggc ggaattgtgc atggaaatac
     4921 gcggcatcgt ccggaacgtc cgtgagcgtg tagttaacct gatagaagag cgtcatgtca
     4981 tcgagaccga tattctccat ggtgatccgg cagtgtttcc ggaacggcat ctcccaatag
     5041 cagttcagac cgctcttcgg attcacgcag accgcgagcg acgatacctg cgcgtacggt
     5101 ccccatgcgt tcgcgaagaa atcaccgaca gggcactcga ccgacggctg tttctgaccg
     5161 tcccaataga tgcgtaggat gctgtgacgc cagtggcccg tcggcgtcat ccaaatctgt
     5221 tgaattgcgc ccggaccttc gatgtcggca atggtgaacg tcgaaccttt ttcgattcgc
     5281 accgccggcg atatcttcca gccttttccg agttcgcgcg actgattgaa ccctaagcct
     5341 tcggtcgcct tgccgccttc gcctttcgca ccggtgaagt tttccgcgct gatcgatctc
     5401 gacttggcct tcgaaacccg cgaaagattc cccatatgca tac
//
LOCUS       DSCC01000037            3194 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020541, whole genome shotgun sequence.
ACCESSION   DSCC01000037 DSCC01000000
VERSION     DSCC01000037.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020541"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            103..2061
                     /locus_tag="ENN56_00395"
     CDS             103..2061
                     /locus_tag="ENN56_00395"
                     /inference="COORDINATES: protein motif:HMM:PF02836.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73976.1"
                     /translation="MKYSYDLSSLTWTITGFTPYLWRFESTPGIGETSVADILSVRAR
                     VPGSVQKTLHDEGLLPNWNEGLNARACEWVENRHWVYMTRIPREWLAASSEDASIRLV
                     CDGLDYGGWVRFGGRQIASFTGTHTPHAFDLTPYLDQVGDDTRLEIIFDCPPRWLGQF
                     GYTSRMTEFKPRYNYTWDWIARLVQIGIWDEVRLEIADAGELTDMAIGTDFDVVRETG
                     ILSVRCDAPDESGWQLRMSLDAVRTERYDLTSGENVILWSDLDVEPWHPNGFGEPTLY
                     DLTLTLIGPDDAEHDRVERRVGFKHVEWQPCEGAPEEADPWICVVNGEPVFLGGVNWT
                     PIRPNFADVTLDDYRKRLETYQQMGCTVLRVWGGGFLEREDFYDLCDELGLLVWQEFP
                     LSSSGLDNYPPDDPESIEELARIATSFVRRRRHHASLLIWCGGNELTTIPAGQTEGMG
                     PPIGMEHPLMQRFAEVVEREDPGTRFVPTSSSGPSFGAESEFGKGVHWDVHGPWKLWD
                     GMDAWEAYWANDDALFRSEVGAPGASSVSLIERYRGDEVSFPASHANPLWRRTAWWVE
                     WKAFIDEHGREPSDVQEYVVWSQHRQSRALEIAARACRDRFPRCGGFIVWMGHDSFPC
                     TANTAVIDFEGEMKPAAHALAAVFRDDG"
     gene            2153..>3194
                     /locus_tag="ENN56_00400"
     CDS             2153..>3194
                     /locus_tag="ENN56_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73977.1"
                     /translation="MTASESTEAGFPPTHAPDIVAEEVIIETEPWNNGAGMSWDYGSR
                     NITRVGDTVFVSVSTPDSNSKPYHNVFWNLYRRVDDGSWEKLADAGAEREREPCPIVA
                     VPDSTGAKRIVLSTTPAIRVSGVHQDGAVETTYNEPRLLVFDAATPSSPPDTLMPAWD
                     RSLGASEHSYRGFAADGRTGALIVTQQVHDPAFHTYSQAYAYRGSSGGWDANGLLRFP
                     MRGCYSAFAVNDRAVHVVAISDEIEPNAEWREYKREITGKYWDYDFRQLFYTWTPDVT
                     KREFSPPITIASADETAGIIRHTDIYVDSADAAYVVYTERNFWHDFMRDRFFPGTPIR
                     IALKVAKLEKGRV"
BASE COUNT          649 a          929 c          978 g          638 t
ORIGIN      
        1 ccccagcgtt gagttcgccc cccctttgcc ccgttcccgc ggattctcta cctcttgtaa
       61 ggcaactacc gtactgatcg agaaccgtac aacggggtcc gcatgaagta ttcctacgat
      121 ctctcctcgc tgacgtggac cattaccggt ttcacgccgt atctctggcg attcgaaagc
      181 acacccggaa tcggcgagac gtccgtcgcc gacattttgt ccgtgcgcgc gagagttccc
      241 ggctccgtgc agaagacact gcacgacgaa ggtctattgc caaactggaa cgaagggctc
      301 aacgcgcgag cgtgcgagtg ggttgaaaat cggcactggg tgtatatgac gcgcatccca
      361 cgcgaatggc tcgcagcgtc ctccgaggat gcgagcattc ggctcgtctg cgatggactt
      421 gactacggcg gatgggtacg gttcggcgga cggcagattg catcgttcac cggcacgcat
      481 acgccgcacg cattcgatct gactccgtac ctcgatcagg tgggcgacga tacacgactc
      541 gagatcatct ttgattgtcc gccgcgctgg cttggtcagt tcggatacac ctcgcggatg
      601 acggaattca aaccgcgcta caactataca tgggactgga tcgcaaggct cgttcagatc
      661 ggcatttggg acgaggttcg tctcgagatc gccgacgccg gagagcttac ggacatggcg
      721 atcggaacgg atttcgacgt cgttcgcgag accggaatcc tcagcgttcg ctgcgacgca
      781 cccgatgaat cgggctggca gcttcgcatg tcgctggatg cggtgcgaac ggaacgctac
      841 gatctcacgt ccggagaaaa tgtcatcctc tggtccgacc ttgacgtcga accgtggcat
      901 ccgaacggtt tcggtgaacc tacgctttac gacctcacct tgacgctgat aggaccggat
      961 gacgccgaac acgaccgcgt cgagcggcgt gtcggtttca aacacgtcga atggcaaccg
     1021 tgcgaaggcg cgcctgagga agctgatccg tggatatgtg tcgtcaacgg cgaacccgtg
     1081 ttcctcggcg gtgtaaactg gacgccgatt cgtccaaact tcgccgatgt aacgcttgac
     1141 gactaccgga aacgcctcga gacgtaccag cagatggggt gcaccgtatt gcgggtctgg
     1201 ggcggcggat tcctcgaacg cgaggatttc tacgacctct gcgacgaact cgggctgctg
     1261 gtctggcagg aattcccctt gtcgtcgtcc ggactggata attacccgcc ggatgatccg
     1321 gaatcgatag aagaacttgc gcgaatcgcg acgtcatttg tccgtcggcg tcgtcatcat
     1381 gcgtcgttgc tcatctggtg cggcgggaac gaattgacga caatcccagc cggtcagacc
     1441 gaaggcatgg gacctccgat cggtatggaa catccgctca tgcagcgttt tgccgaagtg
     1501 gtcgagcgcg aagatcccgg aacgcgattc gtacctacgt cgtcgagcgg accgagtttc
     1561 ggcgccgaat cggagttcgg caaaggcgtc cactgggacg tgcacggtcc ttggaagctg
     1621 tgggacggca tggacgcgtg ggaagcatac tgggcgaacg atgacgcgtt gtttcgcagc
     1681 gaagtcggtg cgcccggcgc gagtagcgta tccctcatcg agcggtatcg cggtgacgag
     1741 gtgtcgtttc cggcgagtca tgcgaacccc ttatggcgtc gaacggcgtg gtgggtcgag
     1801 tggaaagcgt tcatcgacga gcatggccgc gaaccatcgg acgtgcagga atacgtcgta
     1861 tggagccagc accgacaaag tcgcgcgctg gagatcgccg cgcgggcatg tcgcgatcga
     1921 ttcccacgct gcggtggttt catcgtatgg atggggcatg acagcttccc gtgcaccgcg
     1981 aacaccgcag tcatcgactt cgaaggcgag atgaagcccg ccgcacacgc gcttgccgcc
     2041 gtgttccgag atgacggctg acgctcggat tcactccgcg gacttttcgg gaatgaagaa
     2101 attgattgcg ctaaccacac tactaatcac tcagttggga attggaggag ttgtgaccgc
     2161 gtccgaatct actgaagccg gattcccgcc gacgcacgcg ccggatatcg ttgcggagga
     2221 agtgatcatc gaaacggagc cgtggaataa cggcgccggg atgagttggg attacggctc
     2281 gcgcaacatc acccgcgttg gcgacaccgt attcgtaagc gtgagcacgc ccgattcgaa
     2341 ctcaaagccg taccacaacg tgttctggaa cctctaccgt cgcgttgatg acggctcatg
     2401 ggaaaagctc gccgacgccg gcgccgagcg ggaacgcgag ccctgcccaa tcgtcgcggt
     2461 gcctgattca acgggtgcga agcgcatcgt cctctcgaca acacccgcca ttcgcgtgag
     2521 tggcgtccat caggacggag ccgttgagac cacgtacaac gaaccgcgct tgctcgtttt
     2581 cgacgctgca acgccgtctt cgccgccgga caccctcatg ccggcgtggg accggtctct
     2641 cggcgcatcc gagcactcct atcgagggtt tgccgccgat ggtagaacgg gcgcattgat
     2701 cgtcacccaa caggtgcacg atccggcgtt tcatacgtac tcacaggcgt acgcctaccg
     2761 cggctcgtcg ggcggatggg atgcaaacgg gttgcttcgg tttcccatgc gtgggtgcta
     2821 ctcggcgttc gcggtgaatg accgcgcggt tcacgttgtc gccataagtg acgagatcga
     2881 gccgaatgcg gagtggcgtg agtataagcg cgaaatcacc gggaagtatt gggactacga
     2941 tttccgccag ttgttctaca catggacgcc tgacgtcacg aaacgcgagt tctcgccgcc
     3001 gattaccatt gccagcgccg acgaaaccgc agggattatc cgtcatacgg atatctacgt
     3061 cgatagcgcc gatgccgcgt atgtcgtcta taccgaacgg aatttctggc acgatttcat
     3121 gcgagatcgg ttcttccccg gcacacccat tcgaatcgcg ctgaaggtcg caaagcttga
     3181 aaaggggcga gtga
//
LOCUS       DSCC01000038            3930 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1015456, whole genome shotgun sequence.
ACCESSION   DSCC01000038 DSCC01000000
VERSION     DSCC01000038.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1015456"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..693
                     /locus_tag="ENN56_00405"
     CDS             <1..693
                     /locus_tag="ENN56_00405"
                     /inference="COORDINATES: protein motif:HMM:PF12972.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73978.1"
                     /translation="DLELLPGWPEDNETIRRFRANRRQWLPKLEAAIEVFLGAPELLA
                     ESRLAVRDLVDVTRQWIAERFNIALIGARDAFLAGDTATFDKNAPECRTLLEEQARLA
                     ASWPAYRLDEKIERHRPEFGDDATRAIKHRHVWVTTDPTQHSVPLRDYYRQDLDGLIA
                     DYYAPRVDAYLDFLRTRLADGNLQVSAEEFDALYTPIEEAFIAAPARTLPHDDPVDVV
                     RDVLRRSKGSSE"
     gene            690..2387
                     /locus_tag="ENN56_00410"
     CDS             690..2387
                     /locus_tag="ENN56_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73979.1"
                     /translation="MTDAVHWKPEPDLLATLDNAVPEILASQHPNGRFGTDPWICQDQ
                     NVLLALAAAWSLPESRYHGCENVLDAIVRGGNALIDDMDEEGRWIFRKKDYSEWGMIR
                     MPWTYSRWIRAYQIVREHLRDADRHRWDAALRLGYGKVAEHDIGRTHNIPAHHAMGLF
                     CAGIVFDEPTWRESARSLLHRVVGTQTAEGWWTEHCGPVVTYGMVYVEALGVYCSLTQ
                     SISAPDGFVLDALERAARFHAGWVYPDGSAVEVIDERNPYHTGVRLGNPGFSHTKTGR
                     GFLLHQHNLLRSTGEAFAADYAANMLLFAGSGEAEEIVAGRGTADGALGENALAHRRR
                     PWFVAMSAFTSELTESRWIQDRQNFVSVFHDRCGLILGGGNTKLQPLWSTFTVGDTAL
                     LRQTPGDEEPEFSPPEGLRHVPDSARAVLENGVPVLTLNYEDVRCRVTVRADNPEAAI
                     LTYELLSGSLPSESAERIEAHVPFLPHLGERLSLSSGESVELGEASVAWELDADGWLE
                     HGGWRATLPKGTRVVWPALPHNPYRKDGHAEIDEGRVVAILPLSADSPRAEVTLTVR"
     gene            complement(2394..>3930)
                     /locus_tag="ENN56_00415"
     CDS             complement(2394..>3930)
                     /locus_tag="ENN56_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS73980.1"
                     /translation="SGTYRFSIPLVDLIAPTTQSATYNVAVRIEIIGRDTGLDSLAVV
                     LDTQLAPESLPHVLLGTNEIRVTHESAEPVDALLTYQWREAHGVSLPPASSAPIYPAD
                     GATDVPRDGAFRWTPVVHDSVNLYELEISEYPDMRYPIGPDGNRILWTHFTSNITSYT
                     VPMPSRFESGRTYYWRVRAGKYNRWWGPWSDVWSVSFVGPAAPTDAHIDDADGLTLRW
                     TPAVTGTQPAYYEVYADSVEDFIPETGGYPWLLESNADGNIVHMRAPNLIDTVHASKY
                     PIDLASLEFVPAEFRVIAVDVNGTRSTFTAPIRLHPYAVPDSLHMVETGSEWRYRPVT
                     STMISRLEHMERTGYVERETDRIEWRLLDAPTWLNLVDGELVGTPPEGSVRDTITVTM
                     EGASALTGRTVEHAVSLAISPSDDPSDPDDIPESIALAVTPNPFEVSAKIALSVPESA
                     VVTISVYNTSGALVTTLIDNTVVSAREHQITWEPGNLASGVYFIRADIGGKIITARML
                     FVR"
BASE COUNT          735 a         1137 c         1304 g          754 t
ORIGIN      
        1 gatcttgaac tcctcccggg atggccggag gacaacgaga ccatccggcg attccgtgcg
       61 aatcggcgac agtggctgcc gaagctcgaa gccgcgatcg aagtgttcct cggtgcgccg
      121 gaattgcttg cggagagccg cctcgccgtt cgcgacctcg tagatgtcac acgccaatgg
      181 atcgccgagc gcttcaacat cgcgctgatc ggtgcgcgtg acgcgttcct cgcgggagac
      241 acggcgacgt tcgacaaaaa cgctcccgaa tgcagaacgc tgctcgaaga acaagcgcga
      301 ctcgctgcga gctggccggc atatcggctc gacgagaaga tcgagcggca tcgcccggaa
      361 ttcggtgacg acgcgacgcg ggcgatcaaa catcgccacg tgtgggtaac caccgatccc
      421 acgcaacact cggtgccgct acgggattac taccggcagg acctcgacgg gctgattgcg
      481 gattactacg cgccacgggt cgatgcgtat ctggactttc tgcggacgcg gctcgctgac
      541 gggaacttgc aggtgagcgc cgaggaattc gacgcgctct acactccgat tgaagaagca
      601 ttcatcgcgg cgccggcacg gacgctccca catgacgacc cggttgacgt ggtgcgcgac
      661 gtgttacgcc gatcgaaagg atcatctgaa tgaccgacgc tgttcactgg aaaccggaac
      721 ctgatcttct cgcgacgctt gataatgccg taccggagat tctcgcctcg caacacccga
      781 acggacggtt cggcaccgat ccgtggatct gccaggacca gaacgtgctg ctcgcgctcg
      841 ccgcggcatg gtcgctaccg gagagccggt atcacgggtg cgagaacgtg ctcgatgcga
      901 ttgttcgggg tgggaatgcg ctgatcgacg atatggacga ggagggccgg tggatctttc
      961 ggaagaagga ctactccgaa tggggcatga ttcgcatgcc gtggacctac agccggtgga
     1021 ttcgcgcgta ccagatcgtc agggagcacc tgcgcgacgc cgatcgtcat cgctgggatg
     1081 cggcgctacg gctcggatac ggcaaggtcg ccgagcacga catcggacga acgcacaata
     1141 tccccgcgca tcacgcaatg ggactgttct gcgcgggtat cgtgttcgac gagccgacat
     1201 ggcgggagtc ggcacgcagt ctgctgcacc gcgttgtcgg tacccagacg gccgaaggat
     1261 ggtggacgga gcactgtgga ccggtcgtga cgtatggcat ggtgtacgtg gaagcgctcg
     1321 gagtgtattg ctcgctgacg cagtcgatca gtgcgccgga cggcttcgtg ttggatgcgc
     1381 tcgagcgcgc ggcacggttt cacgccggtt gggtctaccc tgatggcagc gcggtggaag
     1441 taatcgacga gcgcaatccg tatcacaccg gcgtgcgact cggtaatccg ggattcagcc
     1501 acacgaagac cggtcgcggg tttctgctcc accaacacaa tctcttgcgt tctaccggcg
     1561 aggcgtttgc tgctgattac gctgcgaata tgctgctttt cgcggggagt ggcgaggcgg
     1621 aagagatcgt cgcgggcagg ggtacggcgg acggtgcgtt gggcgagaac gccctcgcgc
     1681 accggcgtcg cccgtggttc gttgcgatgt ccgcgttcac gagcgaactg acggaaagcc
     1741 ggtggattca ggaccggcag aacttcgtca gcgtgtttca cgaccgatgc ggcctgatcc
     1801 tcggtggtgg gaatacgaag cttcagccgc tctggagtac gttcaccgtc ggcgatacgg
     1861 cactcctccg ccaaacaccg ggtgatgagg aaccggagtt ttccccgcct gagggtctcc
     1921 gacacgtgcc ggactcggcg cgcgcggtcc tcgaaaacgg tgtgcctgtg cttacgttga
     1981 attacgagga cgtgcgatgt cgcgtaacag tgcgcgcgga caatccggaa gcagcaatac
     2041 tgacgtacga actgctcagc ggttcgctgc ccagcgaatc ggcggagcgc atcgaggcgc
     2101 acgtcccgtt tctcccgcat ctcggcgaac ggctgagtct atcatcgggc gaatcggtgg
     2161 agttgggcga ggcgagtgtc gcgtgggagc tcgatgcgga tgggtggctg gagcacggcg
     2221 gttggcgcgc gacgcttccg aaaggcacgc gcgtcgtctg gccggcgctt ccgcacaatc
     2281 cgtatcgcaa ggacggacac gcggagatcg acgagggacg ggtcgtggca atactaccgc
     2341 tctcggcgga ttcgccgcga gcggaggtca cgctgacggt ccggtgacga ctgctaccgg
     2401 acgaacagca tccgcgcggt gataatcttg ccgccgatat cggcacgaat gaagtagacg
     2461 ccggatgcga gattacccgg ttcccatgtg atctgatgtt cgcgcgccga taccaccgtg
     2521 ttgtcgatca gtgtcgtcac gagcgcaccg gacgtgttgt acacggagat cgtcacgacg
     2581 gccgattccg gtacgctgag cgcgattttc gcggatactt cgaacggatt cggcgtgacg
     2641 gcgagcgcga ttgattcggg tatgtcgtcc gggtccgacg gatcgtccga tggcgatatc
     2701 gcgagcgaaa ccgcgtgctc taccgttctt ccggtgagag cgctcgcacc ttccatggta
     2761 acggtgatgg tgtcgcggac tgagccttcg ggaggcgttc ccacgagctc tccgtcgacg
     2821 agattcaacc acgttggtgc gtcgaggagt ctccattcga tacgatcggt ttcgcgttcg
     2881 acgtatccgg tcctttccat gtgctcgagc cgcgagatca tcgtcgaggt aaccggtcga
     2941 tacctccact cgctaccggt ttccaccatg tgcaacgagt ccggtaccgc gtacggatga
     3001 aggcgaatcg gcgcggtgaa cgtgctgcgc gtgccgttca cgtcgacggc tatcacccgg
     3061 aactctgcgg gaacgaattc gagtgacgcg agatcgattg ggtacttgga cgcatggacg
     3121 gtatcgatca ggttcggcgc gcgcatatgc acgatgtttc cgtcggcatt tgattcgagc
     3181 aaccacggat atccgccggt ctccggtatg aagtcctcaa cggaatccgc gtagacttcg
     3241 tagtaggcgg gttgcgttcc ggttacggcg ggagtccatc ggagcgtcag accgtcggcg
     3301 tcatcgatgt gcgcgtccgt cggcgcggcg ggtccgacga acgacacgct ccagacatcg
     3361 ctccatggtc cccaccatcg gttgtatttc cccgcgcgaa cccgccagta gtacgtgcgt
     3421 ccggactcga accgcgacgg catcggcacc gtgtaggacg tgatattcga ggtgaaatgc
     3481 gtccagagga tgcgattccc gtcgggtccg attgggtacc gcatatcggg gtattcgctg
     3541 atttcgagct cgtagagatt taccgagtca tgcacgaccg gtgtccaccg gaatgcgccg
     3601 tctcgcggaa catcagtcgc tccgtcggcg gggtatatcg gtgcggatga agcgggtggc
     3661 agcgatacgc cgtgagcctc gcgccactgg tacgtgagga gcgcgtccac cggttccgcg
     3721 gactcgtgcg tcacccggat ttcgtttgta ccgaggagca cgtgcggaag cgattccggc
     3781 gcgagttgcg tatcgagcac gacggcgagc gaatcgagac cggtatcgcg tccgatgatc
     3841 tcgattcgca ccgctacatt gtatgtggcg gattgtgtcg ttggggcgat gagatcaacg
     3901 agcggaatcg aaaaccggta ggtgccggac
//
LOCUS       DSCC01000039            2276 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032269, whole genome shotgun sequence.
ACCESSION   DSCC01000039 DSCC01000000
VERSION     DSCC01000039.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032269"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..313
                     /locus_tag="ENN56_00420"
     CDS             <1..313
                     /locus_tag="ENN56_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS73981.1"
                     /translation="TGKFTKDTVFESTDHRGNWLKGDLLARAVAEADAVKTAAAPMPA
                     AEASLRFILNQEAVSSVIPGAKNPEQVEKNVAAADGKRLDPAVEQRIRDAVPGTFERR
                     T"
     gene            complement(421..>2276)
                     /locus_tag="ENN56_00425"
     CDS             complement(421..>2276)
                     /locus_tag="ENN56_00425"
                     /inference="COORDINATES: protein
                     motif:HMM:PF07940.11,HMM:PF16332.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HDS73982.1"
                     /translation="DVDAVRNRVAGHDGLRESIVKAATDALTGDLPDPKTQAAETHRP
                     MLPWIHRMVACANAWLLTGDDQFAERAWEIAEVGMIWPNWIGPEHQPLTLDLRSAAVV
                     RSFAVMRDRLDGWLDGERRNRLTAYLVGETKRFVEISDAHSEWWTYSTHNWRTVLDGG
                     YGVAALSVMESLPDDLLRRALKHGIIGSFVILDNGDVDGGWFEGVSYWRYGIGEAVEF
                     IDALYRVSDSAVDLFSHPYLQKTGDFAVFLTWPDGRVYHWGDCGEKINATHLMARLAS
                     ITRRGDWQEYVRRFPAQPTLDTLFWDDPSLEPVPFDTLPKVKHFRGTEVAVLRSGWGD
                     DDLIVGVKAGDTLANHTHLDHGSFVVDVAGHSLIGDAGFWDYATGTYFFNYGEHRYDY
                     PGLDTACHSTILADGRGQEWGPDRHGAIIGTGDFPATDTMPGWAFATVDSSNSYPQLE
                     MFVRYMLLIRPDRVIVIDDIRSHDPARIEWRVVLPYPVRRMPGEPCDPAWPTWVTGPE
                     SGPALTMKWLEPFGDDGLIAQETQLDAIYGARSGPAHHACRMLTVSPLIRQRTTQIAV
                     AMRLGRDGQVVDPGVRVENAYRSLRIIVDTDLGQRTWQIMWGQPGIQAFAQ"
BASE COUNT          453 a          714 c          692 g          417 t
ORIGIN      
        1 cacggggaag ttcacgaaag acaccgtttt cgagtcgaca gaccatcgtg ggaactggct
       61 caagggcgac cttctcgctc gcgccgttgc cgaagccgat gccgtgaaga ccgccgccgc
      121 accgatgcct gcggcggaag cctcactgcg gttcatcctg aatcaggaag cggtttccag
      181 tgtgattccc ggcgcgaaga atccggagca ggtcgaaaag aacgtcgccg cagccgacgg
      241 gaagaggctc gatccggcgg ttgagcagcg tattcgcgat gccgtgcccg gcacgttcga
      301 acgccgcacg tagtgcgaac tcgagcgtcg ctcgagttcg ttggggcgaa gcgcgtaaac
      361 gaaaaaccgg ctcctgtcca cgggtggatg ggagccggtt tgtttccaac gacaattcgt
      421 ctactgcgcg aacgcttgaa tccccggctg tccccacatg atctgccacg tgcgttgtcc
      481 gaggtcggta tccacgatga tgcggagact ccgatacgcg ttctccacgc ggactccggg
      541 atcaaccacc tgaccatcgc gtccgaggcg catggccacc gcgatctgcg tcgtgcgttg
      601 ccggatgagc ggactgacgg tgagcatccg gcatgcatgg tgcgccggac cactgcgcgc
      661 gccgtagatc gcgtcgagtt gcgtttcctg cgcgatgagt ccgtcatcgc cgaacggctc
      721 caaccacttc attgtcagtg cgggacctga ttccggaccg gtgacccacg tcggccatgc
      781 gggatcacaa ggctcgcccg gcatacggcg gaccggatat ggcaagacga cgcgccattc
      841 aatacgcgcc ggatcatgcg agcggatgtc gtcgatcacg atgacgcgat cgggtcggat
      901 cagcaacatg taccgaacga acatctcgag ctgcggatac gaatttgacg aatcgaccgt
      961 tgcgaatgcc catcccggca tggtgtcggt cgctgggaaa tcacccgtcc cgatgatcgc
     1021 gccatggcga tccggacccc actcctgacc gcgaccatcg gcgagtatcg tgctgtggca
     1081 cgcggtatcg agccccggat agtcgtacct gtgctcgccg tagttgaaga aatacgtgcc
     1141 ggtcgcgtag tcccagaaac cggcatcgcc aatgagcgaa tgcccggcga catcgacaac
     1201 gaatgaaccg tggtcgaggt gcgtgtggtt ggcaagcgtg tcgcccgcct tcacgccgac
     1261 gatgaggtcg tcgtcgcccc aaccggaacg caacacggcg acttccgtac cgcggaaatg
     1321 tttgaccttc ggaagtgtat cgaacggcac cggttcgaga ctcggatcat cccagaacag
     1381 cgtatcgagt gtcggttgag ccgggaaccg tcggacgtat tcctgccaat cgcctcgccg
     1441 ggtgatgctc gcgagtctcg ccatgaggtg cgtggcgttg atcttttcgc cgcaatcgcc
     1501 ccagtgatac acgcgtccgt cgggccaggt gagaaatacg gcgaaatcgc cggttttctg
     1561 aagatacgga tggctgaaca aatccacggc gctatcgctc accctgtaga gcgcgtcgat
     1621 gaactctacc gcttcgccga tgccgtagcg ccagtagctc acgccttcga accagccgcc
     1681 gtcaacgtca ccgttgtcga gaatgacgaa cgaaccgata atcccatgct tcagcgcccg
     1741 acggagcagg tcgtcgggga gagattccat gacactcagc gcggcgacac cgtagccgcc
     1801 atcgagcacc gttcgccaat tgtgcgtact gtaggtccac cactccgaat gtgcatcgga
     1861 aatctcaacg aaccgcttcg tctcgccgac gagatacgcg gtgagccgat tacgtcgctc
     1921 gccgtcgagc cagccatcga gacggtcgcg catgaccgca aacgagcgaa cgaccgccgc
     1981 gctccggaga tcgagcgtga gcggctgatg ttcaggaccg atccaattcg gccagatcat
     2041 accgacttcc gcgatttccc aggcgcgttc ggcgaactga tcgtcgccgg tgagcagcca
     2101 cgcgtttgca caggcaacca tgcggtgaat ccagggcaac atcgggcgat gtgtttcggc
     2161 cgcctgcgtt ttcggatccg gcagatcgcc ggtcagggcg tccgtcgccg ctttgacaat
     2221 gctttcgcgc agcccgtcgt ggccggcgac gcgattgcgt accgcatcta catcac
//
LOCUS       DSCC01000040            2056 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037077, whole genome shotgun sequence.
ACCESSION   DSCC01000040 DSCC01000000
VERSION     DSCC01000040.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037077"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="ENN56_00430"
     CDS             complement(<1..1152)
                     /locus_tag="ENN56_00430"
                     /inference="COORDINATES: protein
                     motif:HMM:PF12801.5,HMM:PF13183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDS73983.1"
                     /translation="MIRWRWPVQIALLVVFLALTVAAAWPANLPVISSAWLQWNPLVA
                     GIAAIASRSAWHALAWSLPLVVATMLVGRFFCGWVCPFGTILDLSNRVFSRFRKKNND
                     KIRPRARWKYYTLIGVVTIAGTGSATGLIIDPLVLLTRTITLVFVPLAAWTGYGGVVL
                     ARLLPSQAALSAPFLESPPLYTWMTVTALVFIGIVMLERVERRFWCRNLCPLGALLGI
                     LSRTGLVKRSVEAECTACSLCERDCPMGAIPTTDFTSTHRDECILCMRCSNACSQSAV
                     RFGIADRHDAPGFVPERRQVLVAAAAGIGLGLLSASSTHAAVTPAHVIRPPGSIPEDE
                     FLARCTRCLACVEACPTHGLQPLGLDAGWAAIWSPILVPTVGGCEEPCHN"
     gene            complement(1202..>2056)
                     /locus_tag="ENN56_00435"
     CDS             complement(1202..>2056)
                     /locus_tag="ENN56_00435"
                     /inference="COORDINATES: protein motif:HMM:PF04015.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDS73984.1"
                     /translation="WAHAADASRFDRLPDVVHVTGGGPDTLLQRAITELGGISQYVKP
                     GMSVLINPNVGFPNPPEMATTTDPQLIIAMVDLCKEAGARSITVADYPVRDPDLCFER
                     SGIGDLARLNGVDVIPLNARSPYTPTSIPGAVEVAEVDLATLVHASDILISMPIAKSH
                     ASSGVSFALKNNMGLIQARGPFHARYDLHQAIVDLGKLVKPTLIVTDAQRALVTRGPG
                     GPGRVETPGAVIAGLNAACVDAHTVGIASWYNQSLRPDQVKHIRLAGEQGLGEIDCSR
                     LDIRRISI"
BASE COUNT          430 a          582 c          661 g          383 t
ORIGIN      
        1 gttatggcac ggttcttcac acccgccgac ggtcggaaca agaattggcg accagatagc
       61 ggcccacccg gcgtcgagac cgagcggctg caatccgtgt gtgggacacg cttccacaca
      121 agcaaggcag cgcgtgcatc gagcgagaaa ttcgtcttcg gggatcgatc ccggtgggcg
      181 gattacgtgc gcgggagtga ctgcggcgtg tgtcgatgat gcgctgagga gacccaaacc
      241 gatgcctgcg gctgccgcga caaggacctg acgccgttcg ggtacaaatc cgggtgcgtc
      301 gtgccggtct gcgatcccaa accgcaccgc cgactgcgag caagcgttgc tacatcgcat
      361 gcagaggata cattcgtcgc gatgggtgct cgtgaaatcg gttgtcggta tcgcacccat
      421 cgggcagtcg cgctcgcaga ggctacacgc ggtacactcg gcctcgacgg atcgcttcac
      481 gagtcccgtt cttgagagaa tcccgagtaa ggctcccaac ggacagaggt tccggcacca
      541 aaaccggcgt tctacgcgtt cgagcatgac gatgccgatg aatacgagcg cggtaacggt
      601 catccaggtg tagagtggcg gtgattcgag aaatggtgcg ctgagtgccg cttgcgaagg
      661 tagtagccgt gcgaggacga ccccgccgta tcccgtccac gcggcaagtg ggacgaaaac
      721 aagggtgatg gtgcgcgtta gtaggacaag aggatcgatg atgagcccag tcgccgaacc
      781 tgtaccggcg atcgtgacaa ccccaatcag cgtatagtac ttccaccggg cgcgcggacg
      841 aattttgtcg ttatttttct tgcggaaacg ggaaaacacg cgattcgata ggtcgagtat
      901 ggttccgaac gggcacaccc aaccgcagaa gaagcgtccg acgagcatcg tcgcgacgac
      961 aagtgggagc gaccacgcga gcgcgtgcca agccgagcgt gacgcgatgg ccgcgatgcc
     1021 tgcgacgagg ggattccact gaagccacgc gctggagatt accgggagat tggcgggcca
     1081 cgcagccgct accgtcagcg caagaaagac aacgagtagc gcgatctgga ccggccagcg
     1141 ccagcgaatc atacggttcg agtcctccga atccggcgtt cgtgagcgga aaacggtccg
     1201 ctcaaatgga tatacggcga atatcgagcc ggctgcagtc gatctctccg agaccctgtt
     1261 cacccgcgag gcggatatgc ttcacttggt ccggtcgaag cgactggttg taccacgatg
     1321 cgataccgac cgtatgtgcg tcgacgcacg cggcgttcag tccggcgatt accgctcccg
     1381 gtgtttcgac gcggccgggt ccaccgggtc cacgcgtgac gagcgcgcgc tgggcgtcgg
     1441 tgacgatgag ggtcggcttc acaagcttac caaggtcgac gatcgcctgg tggaggtcgt
     1501 accgtgcatg gaacggtccg cgtgcttgga tcaatcccat attgtttttc aaggcgaacg
     1561 agacgcctga agacgcgtgg gattttgcga tgggcatcga aatcaatata tccgacgcat
     1621 gtacaagcgt cgcgagatcg acttccgcaa cttccaccgc gccggggatg ctggtgggtg
     1681 tgtacggact gcgagcattc aacggtatga cgtccacgcc gttcagtcgc gcgagatcgc
     1741 cgattccgct gcgttcgaag cacaggtccg gatcgcgtac ggggtagtcc gcgacagtga
     1801 tgcttcgtgc accggcttct ttgcagagat cgaccatagc gatgatcagt tgtggatcgg
     1861 tcgtcgtcgc catctctggt ggattcggga atccgacgtt cggattgata aggacggaca
     1921 ttcccggttt tacatactgc gagattccac cgagttccgt aatcgcgcgt tggaggagtg
     1981 tatcggggcc accaccggtg acatgcacaa catccggaag tcggtcgaac cgtgaagcgt
     2041 cagctgcgtg cgccca
//
LOCUS       DSCC01000041            2053 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037170, whole genome shotgun sequence.
ACCESSION   DSCC01000041 DSCC01000000
VERSION     DSCC01000041.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037170"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            158..721
                     /locus_tag="ENN56_00440"
     CDS             158..721
                     /locus_tag="ENN56_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73985.1"
                     /translation="MITRTKRAAVFVVLALATTMWMSGCSDNPTPLQSTSSSVKLLSV
                     SSRAKPLASDQLLTTASLLVDPEVGGMLAVGEPDVYGETKLTIPAGAVSEPTVISMEI
                     SYDGPIVVELQPHGIQFDKTVELAISYKPADLTGIDLDEINVYYFNENLAVPTWERVP
                     NSTSVPGEFEVVAPLSHFSRYAPGSEG"
     gene            737..2050
                     /locus_tag="ENN56_00445"
     CDS             737..2050
                     /locus_tag="ENN56_00445"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13176.4,HMM:PF13424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS73986.1"
                     /translation="MHVLRRYKTWCSPFDRSECAGIEGDMLHAARLKHVLDALRTAAE
                     TASGQSDPKIRSAVVSAGVEQLLAEIAGAENDHTLPERAAEVLGIFEKIGLPQEQSAL
                     MGQVILGIGKTYEQMGNNPQAYESSKHALDLAHAANDSELEAGCLRRMGRVLIRLTRW
                     AEAEANFNRSLSFYDTSHDRRGRAEVLLDLGTLHYHQGDLDSAERLYNEALEIAEESK
                     HVSLAINARNNLGIVANIRGDIESAIAHYQSCVPLAEKNGMDTHLARAYHNLGMAYAD
                     SKNWSDAGDCYERALTIARRNSMGELVGTLHLNRSQMYLELSDLDMAAVSCGRALNLF
                     TSSHDRLSEAEAYRVLGGIFARRNDEITSMEMFKRSLEIAQEAPAPLEIGETYRAMGN
                     ALERLGKRKRAEEALEKSLEYFEQAHAKNDASAAQGDLARIRRPA"
BASE COUNT          505 a          524 c          587 g          437 t
ORIGIN      
        1 gttatttgac aaatcgataa ctcattgaac tccattgatt taccagccct ggtccaagaa
       61 gatgggaatt tggcacgttt cttggaagtg aaaagtgtcg gacagatcac tttcttgcgc
      121 ttcattggaa gcgctcacca agggatggag gttctcgatg atcacacgta cgaaacgtgc
      181 agcggtgttt gttgttctcg cgttggcgac gacgatgtgg atgagcgggt gttcggataa
      241 tccaaccccg ctgcaatcga cgtcgagctc ggtaaagctg ctcagcgtgt cgtcgcgggc
      301 aaagccgttg gcgtcggatc agctacttac caccgcatcg ttgcttgtcg atcccgaggt
      361 cggcggcatg cttgccgttg gcgagcctga tgtttacgga gaaaccaaac tcacgattcc
      421 ggccggtgcg gtatcggaac cgaccgtgat cagcatggag ataagttacg acggtccgat
      481 cgtggtcgag cttcagccgc acggtattca attcgacaaa acggtcgaac tggcgatttc
      541 gtacaagccg gcggacctga cgggaatcga tctcgacgaa atcaacgtgt attacttcaa
      601 cgagaatctc gcagtaccaa cgtgggagcg agtccccaat tcgacttccg taccggggga
      661 attcgaagta gtagcaccgc tatcgcactt ctcgcgatat gcaccgggca gcgaaggatg
      721 acgcacatgt cggtaaatgc acgtattgag gagatataag acatggtgtt cgccattcga
      781 tcgctccgaa tgcgctggaa tcgagggtga tatgctgcac gccgcacgcc tgaagcacgt
      841 gctggatgcg ctgcgaacag ccgccgaaac agcatccggc caatccgatc ccaaaatacg
      901 atcggccgtg gtctcagccg gagttgaaca gctcctcgct gagatcgcgg gggcggaaaa
      961 cgatcatacg cttcccgaga gagcagctga ggtactcggc atcttcgaga aaatcgggtt
     1021 gccgcaagaa cagtctgccc ttatgggaca ggtaatcctt ggcatcggaa aaacatacga
     1081 acagatgggg aataatcccc aagcgtatga atcgtctaaa cacgcgttgg atcttgcaca
     1141 tgccgcgaat gattcggagc tggaggccgg ctgcctccga cgcatgggtc gtgtgctcat
     1201 tcgtcttacc cgttgggcag aagccgaagc aaacttcaat cgaagtctga gtttttacga
     1261 tacatcgcac gatcggcgag gacgagccga agttctcctc gatttgggca cgctccacta
     1321 tcaccaaggc gatcttgatt cggcggagcg tctctacaac gaagctctcg aaatcgccga
     1381 agagagcaaa catgtttctc tcgcgatcaa tgcgcggaat aaccttggta ttgtcgcgaa
     1441 tattcgggga gatatcgaat ccgcgattgc gcactaccag agttgcgttc cgcttgccga
     1501 gaaaaacggc atggatacgc atttggcccg agcttaccat aacctgggca tggcgtacgc
     1561 tgatagcaaa aactggtctg acgcgggaga ttgttacgaa cgcgcgttga cgatcgcgag
     1621 acgaaacagc atgggcgaat tggttggcac tctgcatctc aatcgatcgc aaatgtatct
     1681 cgagctgagt gatcttgata tggcagcggt ttcgtgtggt cgagcactca acctgttcac
     1741 gagttcgcat gaccggcttt cagaagcgga agcgtatcgg gttcttggcg gtattttcgc
     1801 gcgccgaaat gacgagatca cctcgatgga gatgttcaaa aggagtctgg aaatcgcaca
     1861 ggaagcgccc gcaccgcttg agattggcga gacgtaccgc gcaatgggca acgcactcga
     1921 gcgtctcgga aaacgaaaac gagccgagga agcactcgaa aaatcgctcg aatacttcga
     1981 gcaggcgcat gcgaaaaacg atgcctccgc ggctcaaggt gatttggcgc ggattcgtcg
     2041 accagcatag aaa
//
LOCUS       DSCC01000042            1604 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052024, whole genome shotgun sequence.
ACCESSION   DSCC01000042 DSCC01000000
VERSION     DSCC01000042.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052024"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            309..890
                     /locus_tag="ENN56_00450"
     CDS             309..890
                     /locus_tag="ENN56_00450"
                     /inference="COORDINATES: protein motif:HMM:PF01202.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDS73987.1"
                     /translation="MLREKAHGTPAESHRIGTRSIVFIGFMGAGKSKVGRLVAERFGV
                     PFVDTDDLIEARTGRTIADIFKTDGEAGFRRNETEAITEALSGDRKVIALGGGAATRD
                     ENWDVIDRAGAFTVYLKASPITILERVADHTHRPLLANLTRDEMLAKITAMLAEREPN
                     YERARLVIATDNTVDKHATADRVYNILLKALSA"
     gene            887..>1604
                     /locus_tag="ENN56_00455"
     CDS             887..>1604
                     /locus_tag="ENN56_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73988.1"
                     /translation="MSVSRGSPKAAFRQIWTKTFTRAWNRRNQLATATLGFWVPWHTA
                     FAWYDPTFDPFAAFGMSLSDILAIVAEPVYAALILVIASAPSELPWRLTVTRSAWLVP
                     RLWLITVKITGMAIVVPYAVLHVGSAVILTWWETYAAAKTIVVITGASIIWIPLVYFM
                     YVLAAPVLVAQTEAEGWDAGAREVDEPTHQRTGVSTGWALRTSRRFVQRNLGCAILVV
                     VSGQALVFAATLVVPPNTGII"
BASE COUNT          370 a          440 c          452 g          342 t
ORIGIN      
        1 cttctcgcga tgccttcaaa cggtgcggat atacctatag atattgatga tacgtatcgg
       61 tatcgcgtag aaagttggtg gcctgtcgat tctcgtccga caggatgggg cggttcggac
      121 gaagtcgaat cggtctttgc gtcgtgtacg ccgacgaagg attagcaacc gatggctcga
      181 aataaggcgc tcacaccacc gcacttcata tgcgaaacat gcggcaaaga aacaacgcgc
      241 ttgcgccgcg atgttctcga cataaagtat aatgcactcg ggaaaacgcc attctggaac
      301 tgtgaagagt gttacgagaa aaagcgcacg gaacgcctgc tgaaagccac cgaattggaa
      361 cgcgaagcat cgtcttcata ggcttcatgg gtgccggcaa gagcaaagtc ggtcgccttg
      421 tcgccgaacg gttcggcgtt ccgtttgtgg atacggacga ccttatcgaa gctcgaaccg
      481 gtcggacgat tgcagacatt ttcaaaacgg acggtgaagc gggtttccgt cgcaacgaga
      541 ccgaagcgat caccgaagcg ctttctggag atcgcaaagt cattgcgctt ggcggtggcg
      601 cggctactcg tgacgaaaac tgggacgtaa tcgaccgagc cggcgccttt accgtctacc
      661 tcaaggcatc accgatcaca attctcgaac gagtcgccga tcacactcac cgaccgctgc
      721 tcgccaattt gactcgcgat gagatgctgg cgaaaatcac ggcaatgctc gctgagcgtg
      781 agccgaacta cgaacgcgct cgactcgtta tcgcaacgga caatactgtt gataaacacg
      841 cgacggcgga ccgcgtgtac aacattcttc tcaaggcgtt gtctgcgtga gtgtcagtcg
      901 cggatcaccc aaagcggcat tccgacaaat ctggaccaag acgttcactc gtgcgtggaa
      961 ccgccgtaat caactcgcta ccgccacgct cggattctgg gtaccatggc ataccgcctt
     1021 cgcatggtac gatcccacct tcgatccgtt cgcggcgttc gggatgtctc tgagcgacat
     1081 cctcgccatc gttgcagaac ctgtctatgc ggcactcata ctcgtgattg cctcggcgcc
     1141 gtccgaatta ccgtggcgat tgacggtcac tcgcagcgca tggcttgtac cccgtttgtg
     1201 gctgatcacg gtgaaaatta ccggaatggc gattgtcgtg ccgtacgccg tactgcatgt
     1261 gggaagcgcc gtaatcttga cgtggtggga gacatacgca gcggcaaaaa cgatcgtcgt
     1321 cataaccggt gcaagtatca tatggatacc gctcgtttac ttcatgtacg tcttggctgc
     1381 gccagtgtta gtcgcacaaa cggaagccga aggatgggac gcaggcgcgc gcgaagtcga
     1441 cgaaccaacg catcagcgaa ccggggtttc aacgggttgg gcactacgaa cgtcacgaag
     1501 attcgttcag cgtaatctcg gttgcgcgat tctcgtggtg gtatccgggc aggcactcgt
     1561 gtttgcagcg acgctcgtcg ttccaccgaa taccgggata atca
//
LOCUS       DSCC01000043            3268 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1019938, whole genome shotgun sequence.
ACCESSION   DSCC01000043 DSCC01000000
VERSION     DSCC01000043.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1019938"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            238..660
                     /locus_tag="ENN56_00460"
     CDS             238..660
                     /locus_tag="ENN56_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73989.1"
                     /translation="MDLEFAFLCDYADLGNNKLHAMGIGIDTLYLESVPSLHPGFVAV
                     VSIRFQRTEIGEKPSNLVIMGPDGEIVAKADGTLSVKSGHGPGNTSIHRLIVALRPIP
                     IQRFGPYSVRCVVNGQEIANIPFQVVKQRENDRESNGV"
     gene            662..1048
                     /locus_tag="ENN56_00465"
     CDS             662..1048
                     /locus_tag="ENN56_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73990.1"
                     /translation="MQPYMVYQFVQPSAESRFATASEAPATSIDTDSPQVLPVSTIRH
                     TGNTENTTSESYMAADFTITYDFNETQYREDLLAAHEAFREDNEGLSWDEFVAEYFED
                     LRALRESRTESERKKSYEDFRRELGL"
     gene            1052..1321
                     /locus_tag="ENN56_00470"
     CDS             1052..1321
                     /locus_tag="ENN56_00470"
                     /inference="COORDINATES: protein motif:HMM:PF05016.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDS73991.1"
                     /translation="MYQVFLSQRANRDLSSVYRTDRKIAETIDKCIMALTSEPRPRGC
                     KKLDGYEIKDAYRIRIRHYRVIYTVDDANKRVEIEVVQRRSDDYR"
     gene            1515..2201
                     /locus_tag="ENN56_00475"
     CDS             1515..2201
                     /locus_tag="ENN56_00475"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS73992.1"
                     /translation="MPPKRRNVSASTTQKRPVGAVTEPWKIIVLAMFFLGLGMLLGFF
                     MSEYARPNRIASGQSTQQQSVLPGGEEIRALLQHIEEAETQLFADPNNPQVRVHVGNL
                     YYDLALARDQAGDGTNAQEGWVRAVRHYEAARELGVADADMLTDLGTSYYRMNQPEQA
                     VEMYEAALEVNSRNANTWMNLGVVKRQALNDIPGAIAAWQEYLRLDPASANADRVRMW
                     LAQVSGSVPE"
     gene            2635..>3268
                     /gene="valS"
                     /locus_tag="ENN56_00480"
     CDS             2635..>3268
                     /gene="valS"
                     /locus_tag="ENN56_00480"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985790.1"
                     /note="valine--tRNA ligase; ValRS; converts valine ATP and
                     tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I
                     aminoacyl-tRNA synthetase type 1 subfamily; has a
                     posttransfer editing process to hydrolyze mischarged
                     Thr-tRNA(Val) which is done by the editing domain; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDS73993.1"
                     /translation="MAHEHEHELASRYDHHEVESHWYDRWDSSGAFHAEDASERPPFS
                     IVIPPPNVTGSLHMGHALTLTIEDIMIRWHRMRGDNTLWMPGTDHAGIATQMVVERQL
                     QRTEKISRHDLGREKFLEKVWEWKQQYGDRISTQARSLGASLDWERERFTLDAGYQKA
                     VREAFVSLYEEGLIYRAYRLINWCPRCHTALSDLEVEHEEGYAGHLWHIAY"
BASE COUNT          815 a          851 c          897 g          705 t
ORIGIN      
        1 acaccatctt gaatctgtga gtatagtata taccttgcct aaatacctta tgtagcgagt
       61 gagtgcgatt cgcaccggtt gagcgaacag tgtgggtagt ttctgtcgca ccggtgaaag
      121 cgacgaatct cctatcggac gttgccgttg aatgtgttca aacgtattat gaagggcaca
      181 tacatcgccc ttaggcgtta gactacaaca accaacacac ccactggaat tgttgatatg
      241 gatctggaat tcgcttttct ttgcgactat gctgatctcg gcaacaacaa gctccacgca
      301 atggggattg gaatagacac tctttacctc gaatcggtgc ccagtttaca tccaggattc
      361 gtcgcagtag tatcgattcg gtttcaacgc accgaaattg gtgaaaaacc ttcgaatctg
      421 gtgataatgg ggccagacgg agagatcgta gcgaaagccg acggtacact ttcggttaaa
      481 tcaggccacg ggccaggtaa cacgagcata caccggctta ttgttgcgtt gagaccaata
      541 ccgatacaga gattcggtcc gtattcagta cgatgtgtag tcaatggaca ggagatcgcg
      601 aatatacctt ttcaggttgt aaagcaacgt gaaaatgatc gcgaaagtaa tggagtataa
      661 catgcaacca tatatggtct atcaattcgt gcaaccttct gctgagagtc gattcgctac
      721 agcgagtgaa gccccggcaa catcaataga taccgactct ccgcaagtgc ttccagtttc
      781 gacgattcga catactggta atacggaaaa tacgacaagt gagagctata tggctgccga
      841 ttttaccatt acgtacgact ttaacgaaac acaataccgg gaagaccttt tggctgctca
      901 tgaagcattt cgcgaagata atgaggggtt gagttgggat gagttcgtcg cggaatactt
      961 tgaagacctg cgtgctttac gtgagtcgcg tacggagagt gaacggaaga agtcatatga
     1021 agattttcgg agagaattgg ggctctgact tgtgtaccaa gtctttcttt ctcaacgggc
     1081 aaaccgcgat ctgtcgtcgg tataccgtac agaccgtaaa atcgcggaaa ctatcgataa
     1141 gtgtataatg gctctcacaa gtgagccgag accgcgagga tgtaaaaaac tcgacggtta
     1201 cgagatcaag gacgcttata gaatccgaat tcgacattat cgtgtgatct acacagtaga
     1261 cgacgccaat aagcgagtag aaatcgaagt agtacagcgc cgttccgatg actatcggta
     1321 aaagatgcgg tagtgcgacg gtcccactat atctatcaaa accatccgac cattacgtat
     1381 aatccttcgc agcttgtgat caatgaccga ccggtgcgtt gcaccttgac ggtacgacga
     1441 ttttccggga cattctctcg atgtacgttc gcgcgcgaaa gattccgatg ccggtgtaac
     1501 cgaaagacct cagtatgccg ccaaaacgtc gcaacgtctc tgcgtcgacc acgcaaaaac
     1561 ggccggttgg tgccgttacc gagccgtgga agatcatcgt actcgcgatg ttttttctcg
     1621 gtctcggaat gctcctcggc tttttcatga gcgagtacgc gcgtccgaac cggattgctt
     1681 cagggcaatc gacgcagcag cagagcgtgc ttccgggcgg tgaggagatt cgtgcgctgc
     1741 tccagcacat cgaagaagcg gagacgcagc ttttcgccga tccgaacaat ccgcaagtgc
     1801 gggttcacgt cggaaatctg tactacgatc tcgcgcttgc acgtgaccaa gccggggatg
     1861 ggacgaacgc acaggaaggg tgggtgcgtg ccgtccggca ctacgaagca gcgagagagc
     1921 tcggcgtcgc ggacgcggac atgctcaccg atctcggcac gtcgtactat cggatgaatc
     1981 agccggaaca agcggttgag atgtacgaag ccgcacttga ggtgaactca cgtaacgcga
     2041 atacatggat gaatctcggc gtcgtcaaac gacaggcgct caacgacatc cccggcgcga
     2101 tcgcggcgtg gcaggaatat ctccggctcg atccggcaag cgcgaatgcc gatcgtgtgc
     2161 ggatgtggct cgcgcaggta agcggctcgg taccggagta actcgccggc tcgcccgcgg
     2221 attgcctccg cacgagcccg gcgaacaccg tcgggttttt ttcatacggt acacacggct
     2281 ttttcaaaaa acgtatattt caatcacctc atcgatacac tccgaagttg cggtgatggg
     2341 agaaagtgat ttcgatggaa cacctgctgg ttgctgtaca ccgggtctcg cggttaatgc
     2401 gtagcatacg ctgaccgtgg agaccgtccc cgctgagaga gcatcttcac tctacgaacg
     2461 gcgttcaacg aacggcgttc gccgaacgat tcatgtcgtt cgtgcccgat tcgcatactc
     2521 cgctcgtcac gctttgaact gcattctacg agggatgcac gcggttcggt agtaacccga
     2581 acggagaaac cgtaagacaa tactcgtatc accgaaatcg aaggatctat acacatggca
     2641 cacgaacacg aacacgaact cgcatcgcgg tatgaccatc acgaagtgga atcccactgg
     2701 tacgaccgat gggattcctc cggcgcgttc cacgccgaag acgcgtcgga acgtccgccg
     2761 ttcagcatcg tgatcccgcc gccgaacgtg acgggcagcc tccatatggg gcacgcgctc
     2821 acactgacca tcgaagacat catgatccga tggcatcgga tgcgcggaga caacacgctc
     2881 tggatgcccg gcaccgatca tgccggtatc gccacgcaga tggtggtgga gcgccaactt
     2941 cagcgcacgg agaagatctc gcggcacgat ctcggtcgtg aaaagttcct cgagaaggtg
     3001 tgggagtgga aacagcagta cggcgaccgc atcagcacgc aagcgcgttc gctcggggca
     3061 tcgctcgatt gggagcgtga gcgcttcacg cttgacgccg gctatcagaa ggcagtgcgc
     3121 gaggcgttcg tcagcctcta cgaagaggga ctgatctacc gtgcatatcg cctcatcaat
     3181 tggtgtccgc gctgtcacac ggcgctcagc gaccttgaag tagagcacga ggaaggttac
     3241 gccggccatc tctggcacat cgcgtatc
//
LOCUS       DSCC01000044            3022 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022139, whole genome shotgun sequence.
ACCESSION   DSCC01000044 DSCC01000000
VERSION     DSCC01000044.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022139"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..2805
                     /locus_tag="ENN56_00485"
     CDS             <1..2805
                     /locus_tag="ENN56_00485"
                     /inference="COORDINATES: protein motif:HMM:PF02384.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDS73994.1"
                     /translation="DLDAEIVKKRKAGYPLDNIIFEDTERAVLYQNGVETMRVTLDTP
                     QHVADLLNAFFAHNAPHHEDFQRAVDEFKERVPELAKGLVGKIREAHENNTAFQQAFD
                     QLYRLCSTSLNPNIAREAVDEMLVQHLLTERLIRTVFGRDNFSQQNIIAQEVEKVIQA
                     LVSHSFSRSEFLKSLDRFYVAIEKDAAAITDFREKQHFINTVYERFFQGYSVKTADTH
                     GIVYTPQPIVDFMTASVDHVLRTEFDTDLGGDDVVILDPCTGTGNFIVNLLDRIPKEK
                     LADAYRHRLFANEVMLLPYYIAALNIEHRYYELTGSYEPFDGLCFVDTLDMAEERQFS
                     LFTEENTERVKREKDAPITVIIGNPPYNAHQVNENDNRKNRPYDEVEKNIRNSYAKDS
                     SATNKNALYDPYVKFFRWAVDRLGNRDGIVCFVSNNSFVDQFAFDGMRKHLQDDFTRV
                     YHVDLHGNVRLNPKLSGTTHNVFGIQVGVGITIAVRRKHHERHTISYHRVGEEWRKEE
                     KYAWLSQAESIAGVEWTELQPDARNTWLVPEGAEEFAAYLPIGDRDTKGDVGVEAIFR
                     SYSRGVATCRDMVVYDFDLARLKDRMDEFSRVYNAEVARYQAAGSKGDIDDFVDYSQI
                     KWSLGLKNKLKAGNRIDVNDDKFRKTLYRPFTSKYLFFDSVANEALYLQYYFFPTPDS
                     ERENRVITVSDIASRAPVWTALVSDRIVDLHLCASVDSHQCFPFYVYAEDGTNRRENV
                     TDWAASAFRAHYHDETITKWDIFWYVYAVLHHPGYREKFADNLKKELPRIPYASDFRL
                     FADAGRALGELHLGYETVEPWRLEHVWADDMPISWRVEKMRVSKDKRSVVYNDALTLT
                     GIPEEAYGYRLGNRSALEWVIDQYRVKTDARSGIVHDPNGYSDDPQYIVKLLGRVVRV
                     SVETVGIVARTLRYATGSLR"
BASE COUNT          696 a          836 c          889 g          601 t
ORIGIN      
        1 gacctcgacg cggagatcgt caagaaacga aaggccggat accccctcga taacatcatc
       61 ttcgaggata ccgagcgcgc cgtgctctac cagaacggcg tcgaaacgat gcgtgtcacg
      121 ctcgatacgc cgcagcacgt cgcggacctg ctcaacgcgt tcttcgctca caatgcgccg
      181 caccacgagg acttccagcg cgccgtcgac gagttcaagg aacgtgtgcc ggaacttgcg
      241 aaagggctcg tcgggaaaat ccgcgaagca cacgagaaca acaccgcgtt tcaacaggcg
      301 ttcgaccagc tctaccggct ctgtagcacc tcgctcaacc cgaatatcgc gcgggaagcc
      361 gtggacgaaa tgctcgtcca gcacctcctc accgaacgac ttatccgcac cgtgttcggg
      421 cgggataact tctcgcaaca aaacatcatc gcgcaggagg tcgagaaggt cattcaggcg
      481 ctcgtctcgc acagtttcag ccggagcgag tttctcaagt cgctcgatcg gttctacgtc
      541 gccattgaga aggacgccgc ggcgattacc gatttccgcg agaagcagca cttcatcaac
      601 accgtctacg agcggttctt ccaagggtat agcgtgaaaa ccgccgacac gcacggcatc
      661 gtctacacgc cacagcccat cgtcgacttc atgaccgcgt cggtggacca cgtgctcagg
      721 accgagttcg acacggacct cggcggcgac gacgtggtca ttctcgaccc gtgcaccggc
      781 accggcaact tcatcgtgaa cctgctcgac cgcatcccga aggaaaagct cgccgacgcc
      841 taccggcacc gcctcttcgc caacgaggtc atgctgctgc cgtactacat cgccgcgctg
      901 aatatcgagc accgctacta cgaactgacc ggctcctacg agccgttcga cggcctctgc
      961 ttcgtcgata cgctggatat ggcggaagaa cggcaattca gcctgttcac cgaggagaac
     1021 accgaacgcg tcaaacggga gaaggacgcg ccgatcaccg tcatcatcgg gaacccgcca
     1081 tacaacgcgc atcaggtgaa cgaaaacgac aacaggaaga accgaccata cgacgaagtc
     1141 gaaaagaaca ttcggaactc gtacgcaaag gactcgagtg caacgaataa gaacgcgcta
     1201 tacgatccgt acgtgaagtt cttccgctgg gcagtggatc gactcgggaa tcgcgacggt
     1261 atcgtctgtt tcgtctcgaa taacagtttt gtcgatcagt tcgccttcga cggaatgcgg
     1321 aagcatctgc aagacgattt tacgcgagtc taccacgtcg atcttcacgg caacgtgcgt
     1381 ttgaatccga aactcagcgg aaccacgcac aacgtgttcg gcatacaggt tggtgtggga
     1441 attactatcg cggttcgccg gaaacaccac gaaagacaca cgatatcgta tcaccgggtc
     1501 ggcgaagaat ggcggaaaga ggagaaatac gcgtggctct cgcaagcaga gtcgatcgcc
     1561 ggtgttgaat ggacggagtt acagccggac gcgcggaaca cgtggctcgt gccggaaggc
     1621 gcggaggagt tcgcggcgta cctgccgatt ggtgatcgcg atacgaaggg cgatgtcggc
     1681 gtcgaggcga ttttccggtc gtatagtaga ggcgttgcga cgtgtcgcga catggttgtg
     1741 tacgattttg atctcgccag actcaaagac cgtatggacg agttttctcg cgtatacaat
     1801 gcggaagtag cgcgctatca ggctgcgggg agtaaaggcg atattgacga ttttgtggac
     1861 tattcgcaga tcaaatggag tcttggatta aagaacaagc tcaaagccgg taatcgaatc
     1921 gatgtaaacg acgataagtt ccgaaaaacg ctgtatcggc catttacaag caaataccta
     1981 ttctttgata gcgtcgccaa cgaagcgctc tatctccaat actatttctt cccgacgccc
     2041 gacagcgagc gcgagaatcg cgtgataact gtaagcgata tagcgtctcg cgctccggtc
     2101 tggacagcgc ttgtatcaga ccgcatcgtc gatctccatc tctgcgcatc agtagattcg
     2161 catcaatgtt tccccttcta cgtctacgct gaggacggga cgaatcgtcg tgagaacgtg
     2221 acggattggg cggcgagcgc gttccgcgcc cattatcacg acgagacgat cacgaagtgg
     2281 gacatcttct ggtatgtgta tgcggtgttg catcatccgg ggtatcggga gaagttcgcg
     2341 gacaatttga agaaggagtt gccacggatc ccgtatgcgt cggatttccg gttgttcgcg
     2401 gacgcgggtc gcgcgcttgg cgagttgcat ctggggtatg agacggtcga gccgtggcgg
     2461 ttggagcacg tgtgggccga cgacatgccg atatcgtggc gtgtggagaa gatgcgcgtg
     2521 agcaaggaca agcgcagcgt ggtgtataac gacgcgttga cgttgacggg tatcccggag
     2581 gaggcgtatg ggtatcggtt gggcaatcgt tcggcgttgg agtgggtaat cgaccagtat
     2641 cgggtgaaga cggacgcgcg gagcggcatc gtgcacgatc cgaacgggta cagtgacgat
     2701 ccgcagtaca tcgtgaagtt gcttggccgc gtggtgcgtg tttcggtgga gacggtgggg
     2761 attgtagcgc gaacccttcg ctacgctacg ggttcgttaa ggtgaagcgc gaatgctacg
     2821 cattcgttgc cttcacccca cgtcttcgag cggaacggaa tatgcccggc ggataaatcc
     2881 gcgaactctc acatctgttc gagttcgttt gggcgaagac gctccgcgtc ttcgccttac
     2941 gtctccgagc gtagcgcgga gacgcgcgct gcgttgacca ctcagcaccc gcaacggtac
     3001 ctttattcga taccatacga ac
//
LOCUS       DSCC01000045            1640 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1050450, whole genome shotgun sequence.
ACCESSION   DSCC01000045 DSCC01000000
VERSION     DSCC01000045.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1050450"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1435
                     /gene="amrB"
                     /locus_tag="ENN56_00490"
     CDS             <1..1435
                     /gene="amrB"
                     /locus_tag="ENN56_00490"
                     /inference="COORDINATES: protein motif:HMM:PF01875.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HDS73995.1"
                     /translation="FYPGSAAQLRVDVDRMLDDAEDFGIGDRVVALVAPHAGYVYSGV
                     TAALAYRQVRGCTYDVVIVIAPSHRDMFPGVSIKPDGAYATPLGDAKIHRDVAKAIIE
                     QCPDAHADERGHRAEHAVEVQIPFIQQALGDVPIVPIVMLDRSWGVCERLARAIHEAT
                     AELRALVVASSDLYHGASVDECEATDDRTIDAAVHADPREFCEQLEDDTVQACGGGPI
                     AVAKAFAALRGATDARVLGRTNSAEVSGSFTGYVVGYTAIAYSFTDEDLARADSNETP
                     SSATLTPEQRATILSLAKCAVRAAVLENEPPALPTDPSLHGKDQGAFVTLRTGGRRGA
                     GMLRGCIGRMAPSGSPLAEVIIEMARAAALQDSRFMPVEPPELDDITVEVSVLSPLRR
                     VDSPDEVTPGVHGLLISGYGRRGVLLPQVATEYGWGREEFLNETCVKAGLPRNAWRSG
                     NVTIEVFTADVFGDEIPIGRADTASES"
BASE COUNT          306 a          482 c          533 g          319 t
ORIGIN      
        1 attctacccg ggatcggccg ctcaacttcg cgtcgatgtc gatcggatgc ttgacgacgc
       61 cgaggatttc ggtatcggcg atcgggtcgt cgcgttggtc gctcctcacg cgggatacgt
      121 ttacagtggt gtcaccgccg cgttggcgta tcgacaggtg cgcggctgca cgtacgacgt
      181 ggtaatcgtc attgcgcctt cgcaccggga catgttcccc ggtgtcagta tcaaacccga
      241 tggcgcgtat gcgacaccgc tcggcgacgc aaagattcat cgcgacgttg cgaaggcgat
      301 cattgagcag tgccccgatg cccacgcgga cgagcgcggt catcgcgcgg aacacgccgt
      361 cgaggtgcaa atcccgttca ttcaacaggc gctcggagat gtgccgatcg tgccgattgt
      421 catgctggac cgttcgtggg gcgtgtgcga acgcctcgcc cgcgcaatcc acgaggcgac
      481 ggcggagctt cgcgcattgg ttgtggcgtc aagcgacttg taccatgggg cgtcggtcga
      541 tgagtgcgaa gcaaccgacg accgcaccat cgatgcagca gtacacgctg atccgcgcga
      601 gttctgcgaa caactcgaag acgacacggt acaggcgtgt ggcggcggcc cgattgccgt
      661 tgcgaaagcg ttcgcggcgc ttcgcggcgc caccgacgcg cgtgtactcg gtcggacgaa
      721 ctcggccgag gtgtctggaa gcttcaccgg gtacgttgtc ggctacaccg ccatcgcgta
      781 ttcgttcacc gacgaggacc tcgcacgcgc ggattcgaac gagacgccgt cgtcagcaac
      841 actcacgccg gaacaacgtg cgacgattct gtcgcttgca aagtgcgccg ttcgcgcagc
      901 ggtgctcgaa aacgagccgc ccgcgctacc aacggacccg tcgctccacg gaaaggacca
      961 aggagcgttt gtcacgttgc gtacgggcgg gcgccgcggc gcgggaatgt tgcgcgggtg
     1021 tatcggccgg atggcgccgt cggggagtcc gctcgcggag gtgatcatcg agatggctcg
     1081 cgccgcggcg ttgcaggatt cgcgtttcat gccggtcgag ccgcccgagc tggacgatat
     1141 caccgtagaa gtgagcgtgc tttcgccact ccgccgcgtc gattcacctg atgaggtgac
     1201 gccgggtgtt cacggcctct tgatttccgg ctacgggcgg cgcggtgtgc tgctcccgca
     1261 agtcgccacg gagtatggct ggggacgtga ggaatttctc aacgagacgt gtgtgaaggc
     1321 cggattacca aggaacgcgt ggcgctcggg taatgtgacg atcgaagtgt ttactgccga
     1381 cgtattcggt gacgaaatac caatcggccg cgccgatacc gcgtcggaat cgtaaacgca
     1441 tcgcgtcgtg tatctgtgca acgacgttcg actttttata tcttgatacg gtgcgaaatt
     1501 tgcgtttttc actccgccgg aggggagatt cgatggcaaa ggatacgcta acgattaccg
     1561 acaaccgtac cggaaagacg tacgagtttc ccatcgagta cggaacgtac ccgaaatacg
     1621 gtgccacggt atcggccctc
//
LOCUS       DSCC01000046            2876 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023667, whole genome shotgun sequence.
ACCESSION   DSCC01000046 DSCC01000000
VERSION     DSCC01000046.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023667"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="ENN56_00495"
     CDS             complement(<1..1585)
                     /locus_tag="ENN56_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73996.1"
                     /translation="MVLTRAITRLCVAIVVLGSATYSSAEPAVYVEPDHPVYEFIERL
                     SAKRLVEPHVLAIRPKTRNGIAAILREVPRKSTRLTQQDRRRLRFYLGEFADELPRDY
                     PVRESDAAWWTLRATEYEKTHDDDLPWWRNVLRKRTLGERNDVTVRPLVSLGADIAQD
                     TAMTWSRKIRVGGWATFGSAWSAQAMMVEDVEFGDGAAPPPRYGPERGVAFIDGTHGS
                     SFSVDDPQALVTYSGRHAALSGGVFPLEIGHGRHANTVLSDKAPPLPHVRLTVHPTSA
                     LTFTYVHMFLQSGVPDTTSPWYAESDGWQSRHLRKYFVLHRLDYAGRGFEVGLGESVI
                     YGARNVEPMYLIPVVPFRAAQHDVGDLDNLQMFIDGALTAIPYTRVYGSFFIDEMAIE
                     ALTSGDMSHSWWAWQIGLFATDLWGLLPNVDLRAEYTGALPWAYRHRYLWNTYDTYAY
                     RGNDAVVAYPLGFFNGHNSDYTRFDIQWRPVWNVELTGSASLSRRGAEGSIVEQYDTD
                     APAKEFLFGDVTRTYEYRFG"
     gene            complement(1630..2379)
                     /locus_tag="ENN56_00500"
     CDS             complement(1630..2379)
                     /locus_tag="ENN56_00500"
                     /inference="COORDINATES: protein motif:HMM:PF01522.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDS73997.1"
                     /translation="MKYRWWFAGALGGALGAFFGYALASPRSQIIGSSVWHGPRDRRE
                     IALTFDNGPSPDGTERILELLRSEGVKATFFVLGRATETYPHLARAIVDEGHQIGNHG
                     YSHKSLLWAGPTKTAQQLDRGYRAIESACGIGPTIYRPAFGSQNFFMPWLLRQRGWKL
                     VQWAVSAGDWRIHGARKLTNWVRKIRNGDILLWHDGVRSEITTPRTVTIAALSQVIPF
                     LRDRGFSFVTVDQMLGETSRQPVAVPSSATA"
BASE COUNT          574 a          877 c          864 g          561 t
ORIGIN      
        1 caccgaagcg gtattcatag gtgcgcgtca cgtcgccaaa gagaaactct ttcgcgggcg
       61 catccgtgtc gtactgctcg acaatcgaac cttcagctcc acgtcgagat agcgatgccg
      121 acccggtgag ctccacgttc cagaccggtc gccactgtat gtcgaaccgc gtgtagtcgc
      181 tattgtgtcc gttgaagaag ccgagtggat acgccacgac ggcgtcgttc ccgcggtacg
      241 cataggtatc gtacgtgttc cacaggtagc ggtggcggta tgcccaggga agcgcaccgg
      301 tatattccgc acgcagatcg acgttcggga gcagacccca taggtcggtc gcgaacagcc
      361 cgatctgcca cgcccaccaa ctgtgtgaca tatcaccgct cgtcagcgct tcgattgcca
      421 tttcgtcgat gaagaacgac ccatacacgc gcgtgtacgg aattgcggtc agtgcgccat
      481 cgataaacat ctgtaggttg tcgagatcgc cgacatcgtg ttgcgcggca cggaacggaa
      541 cgacggggat gagatacatc ggctcgacgt tccgcgcgcc gtagatcacc gattcgccga
      601 ggccgacctc gaagccgcgt ccggcgtaat cgagccgatg tagcacgaag tacttccgaa
      661 ggtgtcgcga ctgccacccg tcgctctcgg cgtaccatgg gctcgtcgta tccggtacac
      721 cgctctggag gaacatgtgc acgtaggtga acgtgagcgc agatgtcgga tgtacggtga
      781 gtcgcacgtg tggaagcggc ggcgctttat ccgagagaac ggtgttcgcg tgccgtccgt
      841 gcccgatttc caacgggaag actccgcctg aaagtgccgc gtgccggccg ctgtaggtca
      901 caagtgcttg cgggtcgtcc acgctgaacg atgagccgtg cgtgccgtca atgaacgcga
      961 cgccgcgttc cgggccgtat cgtggcggcg gcgcggctcc gtcaccaaac tcgacatctt
     1021 ccaccatcat cgcctgagct gaccatgccg acccgaacgt agcccaaccg ccgacgcgaa
     1081 tcttccgcga ccacgtcatc gccgtatcct gtgcaatatc cgcaccgagg ctgacgagcg
     1141 gccgcacggt aacgtcgttg cgttcgccga gcgtacgttt ccgaagcaca ttccgccacc
     1201 acgggagatc gtcatcgtgc gtcttctcgt attccgtcgc acgcaacgtc caccacgcgg
     1261 catcggattc gcggacgggg tagtcccgcg gcaattcgtc agcgaactcg cccagataaa
     1321 agcgcaggcg acggcggtcc tgttgggtca ggcgtgtgct cttacgtgga acttcgcgga
     1381 gtatcgcggc gattccattc cgcgttttcg ggcggattgc gaggacgtgc ggctcgacaa
     1441 gtcgcttggc gctcaagcgc tcgatgaact cgtagacggg atggtccggt tcgacgtaaa
     1501 ctgcgggttc agcggaggaa tatgttgcgc tgccgagtac gacgattgcg acacacaatc
     1561 gggtaatggc acgcgtgagt accacaatcg tctccctcgc ggcaggtaat acgtcgtcga
     1621 aacgggtttc tacgcggtcg cagacgatgg aaccgcgacg ggctgccgcg acgtttcacc
     1681 gagcatctgg tcgaccgtca cgaaggaaaa cccgcgatcg cgcagaaacg gaatgacctg
     1741 actcagcgcg gcaatggtga ctgtccgtgg cgtcgtgatc tcgctgcgaa caccgtcgtg
     1801 ccagaggaga atatcaccgt ttcggatttt cctgacccag ttggtgagct ttcgtgcgcc
     1861 gtgaatccgc caatcaccgg ccgaaacagc ccattgaaca agtttccagc cgcgctgacg
     1921 gaggagccac ggcatgaaaa agttctgcga tccaaatgcg gggcgataga tggtcggtcc
     1981 gatcccacac gccgattcga ttgcgcgata gccgcggtcg agttgttgtg ccgtcttcgt
     2041 cgggcccgcc caaaggaggc tcttgtgtga atatccgtgg ttcccgattt gatgcccttc
     2101 gtcgacgatc gcgcgcgcga gatgcgggta tgtctcagtc gcgcgaccga gaacgaaaaa
     2161 cgtagctttt acgccctctg atcgcagcag ctcgagaatc cgctccgtgc catcaggtga
     2221 cggtccgttg tcgaacgtaa gggcgatttc gcggcggtcg cgcggtccat gccagacgct
     2281 tgagccgata atctgcgatc gaggcgacgc gagcgcgtat ccgaagaacg caccgagtgc
     2341 acctccaagt gctccggcaa accaccaacg atacttcacg gcaggctcct taccagtctg
     2401 ctaactgtga cacaacggaa tactcatcgg taataggtat acacgaggga cgctgtccgt
     2461 ccaatagtta cgcgtccagc ttgtcgaccg cgaacatgcc atcgtcccca agctcaagtc
     2521 catccgacgg cccaagaaca cgaacagatg cacccgatgt aacgttctcc aacaccggtt
     2581 gcgtcacatc cattcgtccg acgcggagcg tgttgcgtat gcggacgatg cgcgcggtca
     2641 gcggatcgcc tactccagcg gattcgagcg cggcttgcat tgcctcgcgg tccgtcgcga
     2701 atgtgagcgg cagcatgcct ccgcgcggac gtgtcgcggt gatcgaattc gtatacgtct
     2761 tatggcggtc gtatttcgcc gcgacccggt cgagcgtgaa gtccgcaaga ccgattcccg
     2821 cggcgttacc ctcagatttt gccgtcaggt cacgtacgaa cactcggcga ggtgcg
//
LOCUS       DSCC01000047            1843 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043112, whole genome shotgun sequence.
ACCESSION   DSCC01000047 DSCC01000000
VERSION     DSCC01000047.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043112"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(171..662)
                     /locus_tag="ENN56_00505"
     CDS             complement(171..662)
                     /locus_tag="ENN56_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73998.1"
                     /translation="MDSTYHPSASVSLSVGIRTSDVDYRLVAMYSALRGAMRNGIAHT
                     GAAIVEVSTHDLFGYDRFNAFGGGGVGIRRLREGHFERDISLSAPRDIVYQRTGVDEL
                     VYDLVWRTGVRVALFKRANWSLDTEFAYYAAYSLNQPAGTDLHQRADEMYQVGIGVTF
                     LHL"
     gene            complement(821..1351)
                     /locus_tag="ENN56_00510"
     CDS             complement(821..1351)
                     /locus_tag="ENN56_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS73999.1"
                     /translation="MADIRTKIDELIEESYLATLTEEDIRTDLLRFIDVCDRLLRDSH
                     DTNQDVLRQIEEYKSIALRIEKATELADKQEEWAVWSQRDLLAHRAARLKLLVLDTIR
                     EIDGTLADQIERGRFSSLNSDRQETKTATLPPKTQVIRRPSTQLPHEALMPLIRICYA
                     LLTGATVFLIGYRIFH"
     gene            1609..>1843
                     /locus_tag="ENN56_00515"
     CDS             1609..>1843
                     /locus_tag="ENN56_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDS74000.1"
                     /translation="MNFVDTATIEVYSGDGGNGCVSFRREPFVPRGGPNGGDGGKGGD
                     IIIRSDPQLLTLHDLRYRRVYRAKRGEHGKGSLK"
BASE COUNT          412 a          455 c          541 g          435 t
ORIGIN      
        1 gcctgggttc ccgagtagtc gaattcgcac gcctgtccga taatgattgg acccgagccg
       61 atgagcatga tccggtgtat gtctgttctc tttggcatgt agtccccggt ttgtcacgta
      121 tgcgtgttca cagctacacg tggaccgtgc aggtgctacg aagcaaacat ctaaagatgg
      181 agaaatgtga cgccgatacc aacctgatac atctcgtcgg ctcgttgatg taggtccgtt
      241 ccggcgggct gattcaggct atatgcagcg tagtaggcga actccgtgtc taaactccaa
      301 ttcgcacgtt taaatagtgc gacgcgtacg ccggtacgcc agacaaggtc gtatacgagt
      361 tcatcgactc ccgtgcgttg gtaaacaatg tctcgtggag ccgaaagcga gatgtctcgc
      421 tcaaagtgtc cttctcgtaa gcgtcgaatc cctacaccgc cgccaccaaa cgcgttgaag
      481 cggtcgtatc cgaagagatc gtgtgtcgat acttcaacga tcgccgcacc ggtgtgcgcg
      541 atgccattac gcattgcacc gcgtaaggcc gagtacatcg ctacaaggcg gtaatcgacg
      601 tcggatgtac ggatacctac actcagcgat accgacgcgc tcggatgata cgtgctgtcc
      661 atcgcgccgg ttggaaagct tgcgaatgat gcggttcggc ttgcccattc aagagcggag
      721 gatgagatcg ggaagagaaa tagtgcaatg agaagaacga gcagggtgca cggagtatcg
      781 agtcgtggcg aatgagcttg gggacgtggc acgtgcgacc ttagtgaaag atgcgataac
      841 cgatgagaaa aaccgttgct ccggtgagaa gtgcgtaaca tatgcggatc agtggcatta
      901 atgcttcgtg cggaagctgt gtactcggac gacggatcac ttgagttttc ggagggagtg
      961 tcgcggtttt cgtctcttgc cgatcggagt tgaggctgga gaatcgtccg cgttcgatct
     1021 gatccgcaag agtgccgtca atttcccgaa ttgtgtccaa cacaaggagc ttcaaccgcg
     1081 ccgcgcgatg cgcaagaagg tcgcgttgtg accagacggc ccattcttct tgtttgtcgg
     1141 cgagctcggt tgccttttcg attctcaacg cgatcgactt gtattcctca atctgccgga
     1201 ggacgtcttg attggtgtca tgggaatcgc gaagaagacg gtcacacaca tcgataaacc
     1261 ggaggagatc cgtgcgaata tcttcttccg tcagagtcgc aagataggac tcttcgatga
     1321 gctcatcgat ttttgtgcga atatccgcca tggcacgagc actccctgcg tcatggtacg
     1381 gtgcgcggtt gtgcgtactc cctgtttcaa aaaatacaga atggcgaatc gtatcgtcaa
     1441 gcaatgcgcg cggtgatccg gtgatgcgtg agaaccatca tgtgcgtatc gaagcgttgt
     1501 ttgaaacggg agcgctctcg ccgactaacg ttgccgtgca tctacgtgtg ctctatcatt
     1561 acgaagtgat tttcgttcga ctttttcccg tcaagatagg atactccgat gaattttgtc
     1621 gataccgcta caattgaagt gtacagcggc gacggcggca acggttgcgt gagctttcgg
     1681 cgtgaaccgt tcgttccgcg cggtggtccg aatggtggtg acggtgggaa ggggggggat
     1741 atcatcatcc gaagcgaccc gcagttgctc acgctgcacg atcttcgata tcgtcgcgtg
     1801 tatcgtgcga aacgcggaga gcacgggaaa ggttcgctca aaa
//
LOCUS       DSCC01000048            2700 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025702, whole genome shotgun sequence.
ACCESSION   DSCC01000048 DSCC01000000
VERSION     DSCC01000048.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025702"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            373..1182
                     /locus_tag="ENN56_00520"
     CDS             373..1182
                     /locus_tag="ENN56_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDS74001.1"
                     /translation="MTPSEIETFRRTIYDFYRRHARRLPWRETDDPYRVYISEIMLQQ
                     TQVDRVIPKYDAFLFAFPSVHTLARARFTDVLSVWSGLGYNRRALHLHKAAKQIVENH
                     DGEIPSDPSVLRTLPGIGPYTAAAIAVFAYGKPFPLIETNVRRAYIHEFFVNCENVKD
                     GDIMPLVEETLDRDNPREWFYALMDYGAMLKIEVGNANRRSAHYTKQAPFEGSDRQIR
                     GELLRELGRGKMNEHEIPFAIRRDAQRVTSVIADLCNEGFLVRDGDMIRIA"
     gene            1232..1643
                     /gene="rnpB"
                     /locus_tag="ENN56_00525"
     ncRNA           1232..1643
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ENN56_00525"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1679..2455)
                     /locus_tag="ENN56_00530"
                     /pseudo
     CDS             complement(1679..2455)
                     /locus_tag="ENN56_00530"
                     /inference="COORDINATES: protein motif:HMM:PF03372.21"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            complement(2465..>2700)
                     /locus_tag="ENN56_00535"
     CDS             complement(2465..>2700)
                     /locus_tag="ENN56_00535"
                     /inference="COORDINATES: protein motif:HMM:PF02673.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDS74002.1"
                     /translation="AMPVILGPTILTIGDLIVATGVNDGSASLLPLTVGTLAALIAGV
                     FAIRVLLQFVQRGRLSWFAYYCWAVGGLGLVFF"
BASE COUNT          622 a          747 c          754 g          577 t
ORIGIN      
        1 catgcccgag tgacattcag tggtgcgcgt atttaggtgg agaagtgtcc gaacctctac
       61 gccgtgcgta ccagcagcat cttgcatcat gcagaacgtg ctttgggcgt gtcgcgatcc
      121 tgtcacacgc gttacgggac gcgcgcgatc aggagacttc ggagtcatcc gatccgatac
      181 tctggcaccc gcactgtgcg ctcgcacacg gtcacagatg caggctgatt ccgaatcgcc
      241 gcgtgatgtg tatggctcga tcagttcaac cggaatgtgt tagagtggct caatcgggtt
      301 ctcaatcacg atagcgcgac cctcaaccgc ggcttgactc atagtgcaga cgcgaattta
      361 ccccatcgtc ctatgacacc ttccgagata gaaacattcc gtcggaccat atacgatttc
      421 tacagacggc atgcgcgacg ccttccgtgg cgagaaactg acgaccccta tcgcgtttat
      481 atttccgaga ttatgctcca acagacgcag gttgatcgtg tgattcccaa atacgacgcg
      541 tttctttttg cgtttccgtc agttcatacg ctcgcccgcg ctcggtttac cgatgtcttg
      601 tcagtttgga gcggccttgg atacaaccgg cgtgcgttac atttacataa agcggctaag
      661 cagatcgtcg agaatcacga cggcgagata ccatcggatc cttccgtctt gcgcacactt
      721 ccgggcatcg ggccgtatac ggccgcggcg atcgcggtat tcgcatacgg taaacccttt
      781 cccctgatcg aaacaaacgt acggcgtgct tacatccacg agttcttcgt gaattgtgaa
      841 aacgtcaaag acggtgacat catgccactc gtcgaagaga cactggatcg ggataacccc
      901 agggagtggt tttacgcact gatggattac ggtgcgatgc tgaaaatcga agtcggaaac
      961 gctaatcgcc gaagcgctca ttatacaaaa caggcgccgt ttgaaggctc tgatagacag
     1021 attcgcggtg agctacttcg cgagctcggc cgcggaaaga tgaatgaaca cgaaattccg
     1081 ttcgcgattc gcagagacgc acagcgagta acgagcgtta tcgccgacct ctgcaacgag
     1141 ggatttctcg tgcgcgacgg cgacatgatc cgaatagcct gatgacggat atcgctcact
     1201 ccatggaagt tcggtatctt aaggaagcgg tacggagggt cacgtggtcg caccaagtcc
     1261 gtttctaaga cttggtgagg aaagtccgaa caccgcaggg caggatgccg ggtaactccc
     1321 gggcggcgtg agccgacgga cagtgcaaca gaaaacatac cgccaacgtc gcggcaacgc
     1381 gacacggtaa gggtgaaatg gtggggtaag agcccaccgc ggacccggta acgggttcgg
     1441 cactgcaaac cccatccggt gcaaagccaa ataggggcgg aatcgaggta gcccgcttcg
     1501 tcagcggatt cggtcctccg aatccgacgc ccgggtaggc tgcgcgcgag ggacgcggta
     1561 acgcgtctcc cagatagatg acctcggcga ttccgcgagt gtgggatcgt gaacagaatt
     1621 cggcttatcg accctccgtg tccgtacctt cgatgaccac gtcagcacac actggaaaat
     1681 gatccgatgg gtatttcccg cctcgctcga acgtaaccgt ttcaacattc ctgacgtgaa
     1741 cgccgtttcg acagagtatc cagtcgattc tgtgattctc gtgtttcggt tggccggtaa
     1801 agcgatggac tgtcgctgtg ggtcctaccg ggttcgtaac cttcgtccac gagtcctcga
     1861 aatcccctcg cgaaccgttt ggtgcagctt cattaccggt gagaacccga tatggagcgg
     1921 aatctggcac cgaattgaag tcgccacaca gaatcgtcgg taccgattcc ccaccgatcc
     1981 gtttcaaaat gagctcagcg ccgcgtacgc gaaggctctc atcaccgagt gggaagtgag
     2041 tactggcgat aaggacacgt tgtgcggtcg cgagatcgtt tacgcggcac cacgttacga
     2101 ctcgagggtg gtcttcgggc atccattcgc gcgaaccggg ggtgtccgga ttgtcggata
     2161 accagaacgt tccggatgat tcgatcatga accgcgtcgt gcgcagcaat atcggattgt
     2221 gttcttcgtt ggacgctcct cgacgttctc ttccaacaga cgcgtagtag ggtgaatctg
     2281 acacgacctc atgcaattgc gtacgatatg tttcctgcat gcagactacg tccgtatcga
     2341 gacgcgccaa caagtccgcg acgaaaggcc ggcgatcctg ccagaggttt ccttcatcgt
     2401 gctctgatgg gaagcggata ttgaacgtga cgacgcgaag gtcaatcgat ttcatgctca
     2461 ccccttagaa gaacacgagc ccgagtccgc caacggccca acagtaatat gcaaaccacg
     2521 aaaggcgtcc tcgttgtacg aattgaagaa gaacgcggat tgcaaaaacg ccggctatca
     2581 acgctgccag tgtgccgaca gtgagcggta gcagactcgc ggaaccgtca tttaccccgg
     2641 ttgcgactat gagatcaccg atcgttagta tcgtcggacc gagtatcacc ggcattgcga
//
LOCUS       DSCC01000049            1791 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044724, whole genome shotgun sequence.
ACCESSION   DSCC01000049 DSCC01000000
VERSION     DSCC01000049.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044724"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..930
                     /locus_tag="ENN56_00540"
     CDS             <1..930
                     /locus_tag="ENN56_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDS74003.1"
                     /translation="PIISDDEVKRLIDDFIAAAKIAKRVGADFVDVKHCHGYLLHEFL
                     GAKTRKGPYGGSFENRTRILREITERIRSEVPGLLIGVRLSAFDLVPYKPDPAQTRGA
                     VLGPGVPEDFSHALPYQYGFGVDENDPTQYDLTETIRFLELCRELDIRLLNLTAGSPY
                     YNPHVQRPASNPPSDGYRPPEEPLVGCARQINVVKQLKANFPDLVLVGTAYSYLQEYL
                     PHVAQYYVRTGAVDSVGLGRMLLPYPSFLADAIAGKPIDERLICRTLSDCTTAPRAGL
                     PSGCYPLDDYYKNNPDSAELKQIKRDRAEKMRK"
     gene            complement(1015..>1791)
                     /locus_tag="ENN56_00545"
     CDS             complement(1015..>1791)
                     /locus_tag="ENN56_00545"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74004.1"
                     /translation="ADFTREVTLPSITDPKAPFPVKTDTEVDAYAKHLKENGVTISAF
                     LLANNLNAADLDAEHAWMERAVRVAHRLGVPAVRIDSAMSGQQELPFEERVAKFVAGI
                     KRLIDATEDAPVDLGIENHGYQGNDPNFLHGVFDGVGSPRLGMTLDTGNFYWRGHPMS
                     KTLKTIEEFAKLAKHTHVKNINYPEETRDQQREMGWEYGRYVSPIYEGDIDHKKVVGF
                     LRAAGYDRDLCIEDESLGKFSEAERKDILMKDAEHLKSLI"
BASE COUNT          354 a          556 c          520 g          361 t
ORIGIN      
        1 ccgattatct ccgacgacga ggtgaagcgt ctgatcgacg acttcatcgc ggcggcgaag
       61 atcgcgaaac gcgtcggcgc ggatttcgtc gacgtgaagc actgccacgg ctaccttctc
      121 cacgaattcc tcggtgcgaa gacgcgcaaa ggcccgtacg gcggctcatt cgagaaccgc
      181 acacgcatac ttcgcgagat aacggaacgg attcggagcg aggttccggg attgctcatc
      241 ggtgtgcggc tgagcgcgtt cgatctcgtc ccctacaaac ctgatccggc gcagacccgc
      301 ggcgcagtgc tcggtccggg cgtaccagaa gacttctcgc acgcgcttcc gtatcaatac
      361 ggctttggcg tcgacgagaa cgacccgaca cagtatgatc tcacggaaac aatccgcttc
      421 ctcgagctct gccgcgaatt ggacatccga ctcctgaatc tgaccgccgg cagtccgtac
      481 tataatcccc acgtgcagcg cccggcgagc aatccgccat ccgacgggta tcgtcctccg
      541 gaagagccgc ttgtgggatg cgcgcggcag atcaacgtcg tgaagcaact aaaggcgaac
      601 tttcccgacc tcgtattggt tggaacggcg tacagctact tgcaggagta tctcccccac
      661 gtggcgcagt actacgtacg caccggcgcg gtcgattcgg tcggtctcgg tcggatgctc
      721 ctgccgtatc cgagcttcct ggccgacgcc atcgccggaa agccgattga cgaacggctc
      781 atttgccgaa cgctctccga ctgcaccacg gcgccacgag ccggtctacc gagcggatgc
      841 tatccgctgg acgactacta caagaacaac ccggacagcg ccgagttgaa gcaaatcaaa
      901 cgagatcgtg cagagaagat gcggaagtag accaacgccc agacccgcgt acaacagaaa
      961 acggaggcgt gatatacgtc gcgcctccgt ttctcgtttg tctctacgtg ctcgttagat
     1021 caggctcttg aggtgctcgg cgtccttcat gaggatatcc ttgcgctcgg cttcggagaa
     1081 cttgcccagc gactcatcct caatacagag gtcgcgatcg tatccggccg cgcggaggaa
     1141 tccgacgacc ttcttgtggt cgatgtcgcc ctcgtagatc gggctgacgt aacggccgta
     1201 ttcccatccc atctcgcgct gctgatcgcg ggtctcttcg ggatagttga tgttcttcac
     1261 gtgggtgtgc ttcgcgagct tcgcgaactc ctcgatggtc ttcagcgtct tcgacatcgg
     1321 gtgaccgcgc cagtagaaat tgcccgtatc gagcgtcatg ccaaggcgcg gcgagccaac
     1381 cccgtcgaat acaccgtgaa ggaagttcgg gtcgttgccc tggtagccgt ggttttcaat
     1441 gccgagatcg accggagcgt cttcagtcgc gtcgatgagg cgcttgatac ccgcaacgaa
     1501 cttcgcaacg cgctcctcaa acggtagctc ctgctggccg ctcatggcgc tgtcgatgcg
     1561 cacagccgga acaccaagcc gatgcgctac acgcaccgca cgctccatcc acgcgtgttc
     1621 cgcgtcgagg tcggcggcgt tgagattgtt cgcgaggaga aacgcgctga ttgtcacgcc
     1681 gttctccttg aggtgcttcg cgtatgcgtc aacttccgta tccgtcttga cgggaaacgg
     1741 cgctttcgga tcggtaatcg agggaagcgt aacttcgcgg gtgaagtccg c
//
LOCUS       DSCC01000050            1978 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039055, whole genome shotgun sequence.
ACCESSION   DSCC01000050 DSCC01000000
VERSION     DSCC01000050.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039055"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..640)
                     /locus_tag="ENN56_00550"
     CDS             complement(<1..640)
                     /locus_tag="ENN56_00550"
                     /inference="COORDINATES: protein motif:HMM:PF05036.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDS74005.1"
                     /translation="MITHAVGTLFSVVRPHTAYPNYRIRVSRTLLPLALVLAAFVFPS
                     QADAAYAVYAGSLTSPTQAQEEAQRINRALTITSATYERATVRGEVFWRVRVGIYRTQ
                     QEVNAIRQQFSNAGLGDTWMREVPDSELPEQSPSVQPQPTPTTRDTVSIDSVAASLVE
                     SISSLARRAVDSLETVSQRRLEHLLQRMQESFAEEVFRQVEEDRTQERSMYVT"
     gene            complement(645..1127)
                     /locus_tag="ENN56_00555"
     CDS             complement(645..1127)
                     /locus_tag="ENN56_00555"
                     /inference="COORDINATES: protein motif:HMM:PF03259.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HDS74006.1"
                     /translation="MSGGLVIYDEQLARINEVIGRLLKEGDAKCALVVNRDGRTISKQ
                     GFTRNMDTDQLGALVAGAFSATSRVASLLGEPEFTVMFHQGKHDHIHITLIDDRTLLV
                     VTFDDRTTVGMVRLVTKAVCDSLAEVLAVIYQNPEQEEELASADEIANSFDDVFGKQE
                     "
     gene            complement(1221..1925)
                     /locus_tag="ENN56_00560"
     CDS             complement(1221..1925)
                     /locus_tag="ENN56_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HDS74007.1"
                     /translation="MPTIDHANKEISCKIVYYGPGLCGKTTNLELIHRDVPGQSKTNL
                     VSLATEKDRTLFFDFLPVTLGEIKGFKIKFQLYTVPGQVYYNATRKLVLSGVDGVVFV
                     ADSQRSKFRENVDMMENLRENLLEYRINLDELPLVLQYNKRDLPDIVPVSELRQALNT
                     RGIPEFEAVAVQGKGVRETLRSIATETLSRVGRRAASTASKQKTPPTGTGSRTEPAKP
                     SEQRRSIFKRRSTGNG"
BASE COUNT          410 a          568 c          541 g          459 t
ORIGIN      
        1 gcgtaacata catggatcgc tcctgcgtgc gatcttcctc gacttgccga aagacttctt
       61 ctgcgaaaga ttcctgcatg cgctgtaaaa ggtgttcgag acgccgttgt gaaaccgttt
      121 cgaggctgtc gacggcgcgt cgggcaagcg acgagatcga ctccacgagc gatgccgcaa
      181 ccgagtcgat actcaccgta tcacgagtcg taggcgttgg ctgcggttgt accgacggtg
      241 attgttccgg cagttcgctg tcgggaacct cacgcatcca cgtatcgcca agccctgcgt
      301 tcgaaaactg ctgccggata gcgttgactt cctgctgtgt tcgatagatc ccaacacgca
      361 cgcgccagaa aacttcgcct cgcactgtcg cgcgctcgta cgttgccgat gtaatcgtca
      421 atgcgcgatt gattcgctgc gcctcttctt gggcttgcgt aggagacgtt agactgcctg
      481 catagaccgc atatgccgca tcggcttgag agggaaacac aaacgcggcg aggaccaatg
      541 cgagcggaag gagtgtgcga gagacgcgta ttcgataatt cgggtacgcg gtgtgcggac
      601 gtacgacaga gaacaaagta ccgaccgcgt gcgtgatcac cagcttactc ctgttttcca
      661 aatacgtcgt cgaagctgtt cgcgatttcg tcggcgcttg caagctcttc ttcttgttcc
      721 ggattctggt aaatcaccgc aagaacttcg gcaagcgagt cacacaccgc cttcgtcacg
      781 agacggacca tgccaaccgt cgtgcggtcg tcgaacgtga cgacaagcag cgtccgatcg
      841 tcaatcaacg tgatgtggat gtgatcgtgc ttcccctgat gaaacatgac cgtgaattcg
      901 ggctcaccga ggagagacgc cacacggctc gttgcggaaa aggctcccgc aacaagagct
      961 ccgagttgat cggtgtccat attgcgcgtg aacccctgct tggaaatcgt gcgcccgtct
     1021 ctattcacga caagcgcgca tttggcgtcg ccttccttca gaagccgtcc aattacttcg
     1081 ttgatacgcg ccaattgttc gtcgtagatg accaatccac cgctcatcgt gtatcctccg
     1141 tcaccagcga tcgccgtagc atcgtacggc gcgggtttcg ctgaagcgat caacgttccg
     1201 ttcagctcat tttcgagata tcaaccgttg cccgtcgacc gtcttttgaa tatcgaccgc
     1261 cgctgctccg aaggcttcgc gggctctgtt cgcgaaccgg tcccggtcgg tggggttttc
     1321 tgtttcgatg ccgtcgatgc ggcgcgccgt ccgacgcgcg acaacgtttc cgtagcgatt
     1381 gaacgcagcg tctcgcggac gcctttccct tgcactgcga ccgcttcaaa ctccggaata
     1441 ccacgcgtgt tgagcgcttg ccgcaattcc gatacaggga cgatgtccgg tagatcgcgc
     1501 ttgttgtact gcagaacgag cggcagttcg tcgaggttga tgcggtattc aagcagattc
     1561 tcgcggagat tctccatcat gtcgacgttc tcgcggaatt tcgaccgctg cgaatcggcg
     1621 acaaacacaa caccatccac accgctgaga acaagcttcc gcgtcgcgtt atagtacacc
     1681 tgacccggta ccgtatacaa ttgaaacttg attttgaagc cttttatctc gccgagcgtg
     1741 accggaagaa agtcaaaaaa cagtgtccgg tctttttcgg tggcgagtga aacgagattc
     1801 gtcttactct ggccgggaac gtcgcgatgg atgagctcga gattcgttgt ttttccgcaa
     1861 agccccggcc cgtaatacac aatcttgcag ctgatttcct tgttcgcgtg atctatcgtg
     1921 ggcattgccg tggcctccag tcagatgccg tgggcatgga atactcgcgg aacgaaac
//
LOCUS       DSCC01000051            1784 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044970, whole genome shotgun sequence.
ACCESSION   DSCC01000051 DSCC01000000
VERSION     DSCC01000051.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044970"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            292..>1784
                     /locus_tag="ENN56_00565"
     CDS             292..>1784
                     /locus_tag="ENN56_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74008.1"
                     /translation="MNPIRIALPAKSSPRVERNVAIVHRMLRERFDVNLEVASTDIAN
                     VKLVIDPALGADRFSITSDETTTITVGDDRQCAAAFGNWLRGLRRDSDGLRYAGGDVD
                     VTPECSIRGAYLANHFYNFYEAAPIEDVIHYIEELALLGYNTIGLIIPMFQVASFEDE
                     AGRRSFERTNTLFREVRAIGLSACLLDNLNGGFYTAPKEIHAVPPRDDFGRRGWHGLQ
                     VCPSKPAGHAYLIETRIQLLDLFAETGIDSFVFWPYDEGGCGCEQCAPWGARGYLKLG
                     REVAEIARAKYPGIKIILSTWMYDTPYDGEWEALSAEMEREPWLDAILADAHEDFPSY
                     PLEHGSPGGISLINFPEISMWGQGPWGGFGANPLPGRFERLWNQARHILDGGLLYSEG
                     IFEDLNKALYAGWYRNRDRSAESTLREYLAWETGVDADDALDTIVAAIHIMEQNHKRD
                     EIGESAEEAYRLLGDVEARMSPGAAKRWRWRLLMLRATIDRELYRHGG"
BASE COUNT          369 a          513 c          547 g          355 t
ORIGIN      
        1 atgttcacgt tcagtttgtc gtcggacggg cacgcacgcg atacggtcgc gttttccgag
       61 gatctcgaaa cgtggcataa agcgacgcgt ccgctggtgg atgtcggtcc gcgcgggagc
      121 gttgacgaac gatacgcaca caaaccatcg atgtttgcta ccaatggagc gctttaccac
      181 ttttacacgg ccgtggcgcc gaatccatca ggacggctcg gcgaagtgaa tacccacgaa
      241 atacgcggca tcagcgttgt cgcatcttgc ccattctgag gagctttccg tatgaatccg
      301 atccgtatcg ccctaccggc gaaatcgtca ccgcgcgtcg agcggaatgt cgcgatcgtt
      361 catcgcatgt tgagagaacg attcgacgtc aaccttgagg tcgcgagtac cgatattgcc
      421 aatgtgaagc tcgtcatcga ccctgcgctt ggagcggatc gtttttcgat tacatcagat
      481 gaaacgacga cgatcaccgt cggcgacgac cgccagtgcg ccgcggcatt tggcaactgg
      541 ttacgcggac ttcgccgtga ttcggatgga ctccgctatg ccggcggcga cgtggatgtg
      601 acgcccgagt gttccatacg cggtgcgtat ctcgcaaatc acttctacaa cttctacgaa
      661 gccgcgccga tagaagacgt gatccattac atcgaggaac tcgcgcttct cggatacaac
      721 acaatcggcc tcattatccc aatgtttcag gtcgcttcat tcgaagacga ggcggggcga
      781 cgctcattcg agcgaacgaa cacgctgttc cgtgaagtgc gtgcgatcgg cctttccgca
      841 tgtctgcttg acaatctcaa cggtgggttt tacacggcgc cgaaggagat tcacgcggtg
      901 cctccgcgcg acgatttcgg acggcgtggg tggcatgggt tgcaggtatg cccgtcgaag
      961 ccggcgggac atgcgtatct catcgaaacg cgcatacaac tcctcgatct cttcgcggaa
     1021 acgggcatcg actctttcgt attctggccg tatgacgaag gcgggtgcgg ctgcgaacag
     1081 tgcgcgccgt ggggtgcccg aggatatctc aagcttgggc gcgaggtggc cgaaatcgcg
     1141 cgcgcgaagt atcccggcat caagatcatc ctttccacgt ggatgtacga cacgccgtac
     1201 gacggcgaat gggaagcact cagcgcggag atggagcgcg aaccgtggct cgacgcgatc
     1261 cttgccgacg cgcatgagga cttcccgtcg tatccccttg aacacggcag ccccggaggt
     1321 atatcgctca tcaacttccc ggaaatcagc atgtggggac agggaccatg gggcgggttc
     1381 ggcgcgaacc cgctaccggg ccggtttgag cgcctctgga accaggcgcg ccatatcctc
     1441 gacggcggat tactctactc ggaaggcatc ttcgaggacc tcaacaaagc cctgtacgcc
     1501 ggttggtacc ggaaccgcga ccggagcgcc gaatcaacgc ttcgtgagta tctcgcatgg
     1561 gagacgggtg ttgacgccga cgacgcactc gacacgatcg tcgcggcgat tcatatcatg
     1621 gagcagaatc acaaacggga cgagatcggt gaaagtgccg aggaagccta tcgactgctg
     1681 ggcgacgtgg aagcgcggat gtctcccggt gcggcgaaac gatggcgctg gcggttgctc
     1741 atgctccgcg cgacgatcga ccgggagctg tatcgccatg gtgg
//
LOCUS       DSCC01000052            2603 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026972, whole genome shotgun sequence.
ACCESSION   DSCC01000052 DSCC01000000
VERSION     DSCC01000052.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026972"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="ENN56_00570"
     CDS             complement(<1..1029)
                     /locus_tag="ENN56_00570"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74009.1"
                     /translation="MSCPRRFFGAKSAFWTAFMSYERGIAAIHLEMPDSIPHTEYVSH
                     PRLMERVTGLSANHPDIGPEFYRKWDFDFIWRTDSPEFPNAPRAWMGSARYSEEQELK
                     PAEYPFTTVEEVLAFDPIERIGIPDRETTRLIIERSLGDTRSRFDTVVVPTGYYNTVF
                     TWCIVTFGWDLFLLAAKDDSDRFDTILDGFAQLSQPIFEAAAETNAEAFICHDDIVWT
                     AGPVFRPEWYRRYIFPRYRKLWAPLIEAGIPILFCSDGNFTEFVDDIADAGARGFIFE
                     PHTDLEYIAERYGQTHVIIGNADCRILTYGSSDDIRNEVERCTRIGKPLPGYFYAVGN
                     HIPYNVPID"
     gene            complement(1057..1845)
                     /locus_tag="ENN56_00575"
     CDS             complement(1057..1845)
                     /locus_tag="ENN56_00575"
                     /inference="COORDINATES: protein motif:HMM:PF00657.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HDS74010.1"
                     /translation="MSAMRGNAHENLSVFATSRVGGPVGSTPACRQIHTRSIAFTHST
                     QANGVSMNPINDGQTFLFIGDSITDCGRHGPQAPYGDGYVSLFRELFIAKYPERRVTF
                     INKGIGGNTVEDLNNRWEDDVMPIKPDWLSIKIGINDLHRTLGKTENAVPPKRFAELY
                     DRIIARTFAVRKSELILVSPFYISRDFESDSFRSKVLAFLPEYIEVVEKMASKYDARY
                     VPMQDIYQKHLEIREADVFCPEPVHPNRTGHMVIAEAIMQAIEK"
     gene            1888..>2603
                     /locus_tag="ENN56_00580"
     CDS             1888..>2603
                     /locus_tag="ENN56_00580"
                     /inference="COORDINATES: protein motif:HMM:PF01408.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74011.1"
                     /translation="MEVIMATKFRWGILGTGNIAKQFARGLASISDAELVAVGSRSRE
                     SADRFGDEFSVPHRHSTYEDLARDPGVDAIYVATPHPMHHENAILCLENDKPVLVEKP
                     FTVNASEAKRVIDTARERGVFCMEAMWTRFLPVIAKLREIVESGEIGDVRMVQANFAF
                     RAQFNPESRLFKPELGGGGLLDVGIYAISFARMIMKNAPVEVYSMADMGKTGVDEQAA
                     IILRYGAERMAMLMCGTRVTT"
BASE COUNT          614 a          683 c          698 g          608 t
ORIGIN      
        1 gtcgataggg acattatacg gaatgtgatt tcccactgcg taaaaatacc cgggtagcgg
       61 cttcccaatc cgtgtgcagc gctccacctc attgcgaatg tcatcgctgc tgccgtacgt
      121 gagtatgcgg caatccgcat tgccaataat cacgtgtgtt tgtccatacc gttccgcaat
      181 gtattcaaga tcggtatgcg gttcgaatat gaaaccacgc gctcctgcat cggcgatatc
      241 gtcgacgaac tcggtgaagt taccgtccga gcagaaaaga atcggaatcc ccgcctcgat
      301 gagcggtgcc cagagtttgc gataccgcgg gaagatatac cttcggtacc attccggacg
      361 aaagaccggt cctgcagtcc acacgatatc gtcgtggcag ataaacgctt cagcattcgt
      421 ttccgccgca gcttcaaaga tcggttgcga aagctgtgcg aatccgtcaa gtatggtatc
      481 aaaacgatca gaatcgtcct tcgcggccag caggaaaaga tcccacccga acgtaacgat
      541 gcaccacgta aagaccgtgt tgtagtatcc cgtcggaaca acaaccgtat cgaatcgtga
      601 acgcgtatcg ccaagtgaac gttctatgat gagcctcgtc gtttcgcgat ccggaatccc
      661 gatacgctcg atcggatcaa acgcgaggac ctcttcgacc gtcgtgaatg ggtattcggc
      721 aggcttaagt tcctgttcct cggaatacct tgcacttccc atccaggcgc gcggggcatt
      781 ggggaattcg ggagaatcgg ttcgccagat gaaatcgaaa tcccacttcc ggtagaactc
      841 aggaccaata tccgggtgat tcgccgaaag accggtcact cgctccatga gtcgaggatg
      901 gctcacgtat tctgtgtgcg ggatagaatc cggcatctca aggtgaattg cagcgatacc
      961 tctttcatat gacatgaaag ccgtccaaaa tgctgatttt gcgccaaaaa aacgtcgggg
     1021 acacgacatt ccatgccccc gacaaacggg tttccatcac ttctcaattg cctgcatgat
     1081 agcttcggcg attaccatat gacctgtgcg gttcggatgt actggttccg ggcagaatac
     1141 atctgcttca cgaatttcga gatgcttctg atagatatct tgcatcggta catatcgagc
     1201 gtcgtatttc gaagccattt tctcgacgac ttcgatatat tcagggagaa acgccaacac
     1261 tttgcttcgg aagctgtcgc tctcgaaatc gcgactgatg tagaatggcg atacgagaat
     1321 gagttccgat ttccgcacgg cgaacgttcg cgcaataatc cgatcgtaga gttcggcaaa
     1381 tcgtttcggc ggcactgcat tttcggtctt gccaagtgta cgatgcaggt cgttaatacc
     1441 gatctttatc gacagccaat ctggcttgat cggcatcaca tcgtcttccc aacgattgtt
     1501 gagatcctcg acggtgttac caccgatgcc cttgttaata aaggtgacgc ggcgctctgg
     1561 gtacttggcg atgaacagtt cccgaaagag cgaaacgtat ccgtcgccat atggcgcctg
     1621 aggaccgtgg cgaccacaat cggtaatgct gtcgccgatg aagagaaatg tctgtccatc
     1681 gttgatcgga ttcatggata ccccgtttgc ttgagtggaa tgcgtaaaag ctatcgaacg
     1741 tgtatgaatc tggcgacaag cgggcgttga gccaaccggt ccgcccacgc gactcgtcgc
     1801 gaagactgat aggttttcgt gtgcgttgcc ccgcatggcc gacatgagcg aactttgtag
     1861 taccgaataa ttggccaata gtcctttatg gaggttatca tggcaacaaa attccgctgg
     1921 ggcatcttag gcacgggaaa tatcgcgaaa caattcgcac gcggactcgc atcgatcagc
     1981 gacgctgaac tcgtagccgt gggatcgcgc tctcgtgaaa gcgccgatcg tttcggggac
     2041 gagttctcag tgccgcatcg tcatagcacg tacgaagatc tcgctcgcga tcccggcgtc
     2101 gacgcgatct atgtcgctac gcctcatcca atgcaccatg aaaacgcaat cctttgcctt
     2161 gagaacgaca agcccgttct tgtggaaaag ccgtttacgg tgaatgcgtc agaagcgaaa
     2221 cgcgtcattg ataccgcgcg tgagcgtggc gtcttctgca tggaggcgat gtggactcgg
     2281 tttcttccgg tgatcgcgaa acttcgcgaa attgtcgaaa gcggcgagat tggtgacgta
     2341 cgtatggtgc aggcgaactt cgcattccgc gcgcaattca atccggaaag tcgactcttc
     2401 aaaccggaac tcggtggcgg cggtctgctc gatgttggaa tctacgcgat atcgttcgca
     2461 cggatgatca tgaaaaacgc gccggttgag gtctactcca tggccgatat ggggaaaacc
     2521 ggtgtggacg agcaggcggc gatcattctc cgttatggcg ccgagcgtat ggcgatgctc
     2581 atgtgcggca ctcgcgtcac gac
//
LOCUS       DSCC01000053            1978 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039050, whole genome shotgun sequence.
ACCESSION   DSCC01000053 DSCC01000000
VERSION     DSCC01000053.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039050"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            345..>1978
                     /locus_tag="ENN56_00585"
     CDS             345..>1978
                     /locus_tag="ENN56_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74012.1"
                     /translation="MNSRMIALPSDPHDAIRIGNDVVGIRVWGPAKRPTLSIGAADIW
                     DRRLFNHRQPRITLERIRELAFSDRLQEIARSPNETVYDIYNRYDFPCPKPGAQLILI
                     VPFAEETTVEKIDEQSVRLRAVGPGKILSVHIRVLLSERIVLLDCDASGLHGHDMAVR
                     VWRHRDTIEPGALIHPTLGDRRSANDFDPLPPPNTFERNRSVFGIRQRFPGEMTFPRG
                     FEFAIGAAIDVPGVIRHANGKIGLGTKLDAEKEGRISHGVHKRYTPINEASGSAATMT
                     FRKLPNRFAIVSAIVTSSDTDDPVGDVTRLLNGVSRDRVNDLLRRDNDDRRKATRVPR
                     AVAEVMDGERIEAPEIVLPSLRRAEGYYGDVAMCSVDSTKWTFQDSALWHADFHLNEI
                     RAEPLLTMGRFEEVAQYANLIKTLLPSAEENAATVYSLPGAMYPLVHYPLRCLGIAHT
                     NLTWEQDIGLNGLVTKPLWLYWRYTGDRSFLEDVAYPVLRACATFCAAYVSEEDDGFL
                     HIAPTVSPEHWGLTENFERNRDCASALTLTKYLFNAA"
BASE COUNT          453 a          525 c          557 g          443 t
ORIGIN      
        1 cgatgtgcgt acggtagtcg attggcacga gagaaacacc cttctcaata cgctgtttcc
       61 gtgtgcgagt gcgatctcat ggaagaaaac gatgcgtcaa tgtccttcga cgatggctcg
      121 gtgcgaatga gttttacacc gtgggagatc cggactctca aagtagtgtt ttagggtatg
      181 ttttcggaga tggcgacgtc ggataccaca ctccacggcc cgacgtcgct ctttcatttt
      241 gctgctgttc tccgacggca catcgagttg accgcgaagt cgcggtgttg gtgccgtcga
      301 tatccggtgg taccgtactc gcgtctaccc aaccgaggac acggatgaac agccgaatga
      361 ttgcactgcc gtcagatcca cacgatgcga tacgtatcgg aaacgatgtt gtgggtatcc
      421 gcgtatgggg tccagcgaag cggccgacat taagcatcgg cgcggctgat atctgggacc
      481 ggcgactttt caaccaccgt cagccgcgta tcacacttga gcgtattcgc gaactcgctt
      541 tttccgatag attgcaggaa atagcacgct caccaaacga gacggtctac gatatctata
      601 atcgctacga ctttccgtgc ccaaagccgg gtgctcaact catactcata gtaccgttcg
      661 cggaggaaac gacagtcgag aaaatcgatg agcaatcggt gcgtttacgc gccgttggtc
      721 ccggcaagat tctctccgtg cacattcgtg tgttgctgag tgagcgcatc gttctccttg
      781 actgcgacgc cagcggctta cacgggcacg atatggcggt gcgtgtttgg cggcaccgtg
      841 atacgataga accgggtgca ctcatccacc cgacactggg agatcgcagg tccgccaatg
      901 atttcgaccc tcttccaccg ccgaacacgt tcgaacggaa tcgttccgta ttcggtatac
      961 ggcagcgttt tccgggtgag atgacatttc cacgtggatt cgagtttgcc atcggcgcgg
     1021 cgatagatgt cccgggcgtg attcggcatg ccaatggcaa aatcggattg ggtacgaagc
     1081 tcgacgccga gaaggaaggc cgtatatcac acggtgtaca caaacggtat acaccgataa
     1141 acgaagcatc cggatctgca gcgaccatga cgtttcgaaa gctcccgaat cgattcgcga
     1201 tcgtatcggc gatcgttacg tcgagtgata ctgacgatcc cgtcggagat gtgactcgat
     1261 tgctgaacgg tgtctcgcga gaccgagtga acgatctact acgccgcgat aatgacgatc
     1321 ggagaaaggc gacacgagtt cctcgcgcgg ttgccgaagt gatggatggc gagagaatcg
     1381 aagcacctga gattgtttta ccatcgttac ggcgagccga aggatattac ggtgacgtgg
     1441 cgatgtgcag tgttgattcc acgaaatgga cgtttcaaga ctcggcgctt tggcacgcgg
     1501 acttccacct caacgaaatc cgtgccgaac ccctgttgac gatgggacgt ttcgaagaag
     1561 tcgcgcagta cgcaaacctt atcaagacac tcttaccaag cgcagaggag aatgcagcca
     1621 cagtgtactc gctccccggc gcgatgtatc cgcttgttca ctatccgctt cgatgcctcg
     1681 gtatcgcaca cacgaatctg acatgggaac aggatatcgg actgaatgga ctcgtgacaa
     1741 aaccgctgtg gctctactgg cggtatacgg gggacagatc gtttctcgaa gatgtcgcgt
     1801 atcctgtatt acgtgcatgc gcgacgtttt gcgccgcata cgtgagcgag gaggacgacg
     1861 gattcctcca tatcgctccg acggtttcgc cggagcactg gggactgacg gagaatttcg
     1921 aacggaatcg tgattgcgca tcagctctga ctctcacaaa ataccttttc aacgcagc
//
LOCUS       DSCC01000054            1941 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040092, whole genome shotgun sequence.
ACCESSION   DSCC01000054 DSCC01000000
VERSION     DSCC01000054.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040092"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..174
                     /locus_tag="ENN56_00590"
     CDS             <1..174
                     /locus_tag="ENN56_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74013.1"
                     /translation="KEIEKTEKYVGSTKKRLENRNFVERAPAEVVEAEREKVSAGEAT
                     IARLRDTLESIAS"
     gene            254..1036
                     /locus_tag="ENN56_00595"
     CDS             254..1036
                     /locus_tag="ENN56_00595"
                     /inference="COORDINATES: protein motif:HMM:PF12686.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDS74014.1"
                     /translation="MYLMYVDESGDPGILDESCDATAQPSIHYILTGLIVADVDWKEY
                     LSALVEIRRNLRRVYGYPIRDELKGSELINPRDNEALRTLNRQKRTELYYDFLNSMTL
                     RLNRARVLNVHVNKAQPKYHATTDSSNIEEWAWKCFIQRFENFLKKDGSQTGIIFADD
                     TNEVKIRRLLRTMRHYNPIPRKYGNTYSAPVANIIEDPVMRDSKQSYFVQVSDMIAHS
                     LYRKLYPKGGYRRYNVDRLFDLVDPVLLKQASVTDSQGIVHL"
     gene            1193..1705
                     /locus_tag="ENN56_00600"
     CDS             1193..1705
                     /locus_tag="ENN56_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDS74015.1"
                     /translation="MERLWAPWRIEYISGQRGRESKTGCVFCDAVEEQDDASRLVLGR
                     GRNVFTIMNLFPYTNGHLMIVPNRHVGDFTMLTNDEYRDIMTAVRDAVDVLGATMNCD
                     GYNVGMNLGRAAGAGIAEHVHMHVVPRWNGDTNFMAVVDDTKVVSEALDDTYRKLRAE
                     YEKRGRVTVG"
     gene            1707..>1941
                     /locus_tag="ENN56_00605"
     CDS             1707..>1941
                     /locus_tag="ENN56_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74016.1"
                     /translation="MATWRHPAMGITITPRNGQRGKLRWYLRIVIYAVALVAVVLVMN
                     ALRGQIPQAFGLTPIRVDALSVARISPDSLDASS"
BASE COUNT          496 a          500 c          540 g          405 t
ORIGIN      
        1 aaggagatcg agaagaccga gaaatacgtc ggttccacga agaagcgact ggaaaaccgg
       61 aacttcgtcg agcgcgcgcc ggccgaggtc gtcgaggccg aacgcgagaa ggtcagcgcg
      121 ggtgaagcga ccatcgcgcg tttacgcgat acactggaaa gcatcgcctc gtagcacacg
      181 ggagaaaacc aaatcgtcga tttgtgtccg gtgagaacac ataacgcgct tgagcggatc
      241 aaaggaatcc accgtgtatt tgatgtacgt cgatgaatcg ggagatccgg gtattcttga
      301 tgaatcgtgc gatgcgacgg cacaaccatc gatacattat atacttaccg ggctaatcgt
      361 cgctgacgtt gattggaaag agtatctgtc ggcgctcgta gaaatccgga gaaatcttcg
      421 acgtgtgtat ggttacccga ttcgtgatga actcaaaggt tccgagctta tcaacccgag
      481 agacaacgaa gctctgagaa cactgaatcg acagaagcgc accgagttgt actacgactt
      541 tctcaactca atgactctgc ggttgaatag agctcgtgtt ctgaatgtac acgtaaataa
      601 agcacaaccg aaataccacg ccacgacgga ttcttctaac atcgaagaat gggcgtggaa
      661 gtgctttatc cagaggtttg agaactttct gaagaaggac ggaagccaaa ccggtatcat
      721 cttcgccgat gatacgaatg aagtaaaaat ccgtcgatta ctgcggacta tgaggcacta
      781 taatcccata ccgagaaagt atgggaatac ctattccgct ccggtagcca acattataga
      841 agacccggta atgcgcgact ccaagcaatc gtacttcgtg caagtgtctg acatgattgc
      901 acattcgctg tatcggaagc tgtaccctaa aggagggtat cgtcgataca acgtggatcg
      961 actattcgat cttgttgatc cggtactcct caagcaggcg tctgtcaccg attcacaagg
     1021 gatcgtacac ctttaacaaa aagcgcccgc ttcaagcggg cgactttttc gcggccgaac
     1081 acgattcatg ggaatcggtc ggtcgcatat tcactatact caaacgtaat aacgctcgat
     1141 tttgtttcat tgtcaatatc aaactcgaac aaccaccgaa aaggacgtga ccatggagcg
     1201 gctatgggcg ccgtggcgga tcgaatacat ctccggacag cggggacgtg agtcgaaaac
     1261 cggctgcgtg ttctgcgacg cggttgaaga acaggacgac gcaagccgac tcgtactcgg
     1321 gcgcggacgg aacgtgttta ccatcatgaa cctgtttccg tacacgaacg gtcatctgat
     1381 gatcgtcccg aatcgtcacg tgggcgattt cacgatgctc accaacgacg agtaccgcga
     1441 catcatgacg gcagtgcgcg atgcggtgga cgtactcggc gcgacgatga actgtgacgg
     1501 atacaacgtc ggcatgaacc tcggacgcgc cgccggtgca ggcatcgccg agcacgttca
     1561 catgcatgtc gtgcctcggt ggaacggcga tacgaacttc atggcggtag tcgacgatac
     1621 gaaggttgtc tccgaagcac tcgacgatac gtaccggaaa ctccgcgcag aatacgaaaa
     1681 gagagggcgc gtcacggtcg gctgatatgg ccacgtggcg gcatccggca atggggatta
     1741 cgatcacacc acgcaacggc caacgcggga agctccgctg gtatctccgt atcgtcatct
     1801 atgcggtggc gctcgtcgcg gtggtgctcg tgatgaacgc gctgcgtggg cagattccgc
     1861 aggcgttcgg cttgactccg attcgcgtcg atgcactctc ggttgcgcgt atatcgcccg
     1921 attcgctgga tgcgtcctca c
//
LOCUS       DSCC01000055            2690 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025830, whole genome shotgun sequence.
ACCESSION   DSCC01000055 DSCC01000000
VERSION     DSCC01000055.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025830"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..352
                     /locus_tag="ENN56_00610"
     CDS             <1..352
                     /locus_tag="ENN56_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_952236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDS74017.1"
                     /translation="LVGWVNYPGLKSHPDNERAKKYLPNGFGAIIGFGIKGGRTAGKK
                     FIESVKLFSHLANIGDAKSLVIHPATTTHQQLTPEEQASSGVTDDYIRLSIGLENIED
                     IIADLDQALAASQA"
     gene            436..1659
                     /locus_tag="ENN56_00615"
     CDS             436..1659
                     /locus_tag="ENN56_00615"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDS74018.1"
                     /translation="MLLIQDEDAVRKLFPPGALEAVNSVGATRPHRVRLVEENDSLPL
                     TVGGKIAPVDVEYEMYGTLNADKSNVVVIFHALSGDAHVAGWDMSAVETGRRWRLRKP
                     GWWDEIVGPNKAIDTERWCVICVNALGSCYGTTGPSSINPATGKPYALSFPIVTVEDW
                     VRLQVMLLDHLGIERVHAVIGGSLGGQQVIEFMMAFPDRTEKAIVLAASSKLSAQGLA
                     FNAVGRHTIMNDPNFSDGDYYDRDVPGQGLAAARMLAHITYLSEIGMETKFGRRLQNK
                     TEPDFDFGIEFEVESYLAYQGRSFVERFDANSYLYITRAMDYYDAAATHGGGNLTEAC
                     SRVTAESMVVSFSSDWLYPPNQCRELAIALSQFHRPVTYIDVPSSYGHDAFLVETEIV
                     GRLVSDFLGREVADG"
     gene            1559..2416
                     /gene="metW"
                     /locus_tag="ENN56_00620"
     CDS             1559..2416
                     /gene="metW"
                     /locus_tag="ENN56_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HDS74019.1"
                     /translation="MCRRRMATTRSSSRRRSSAGLSAISSVGRLPMAERDAIERLVDL
                     LTARTEITGRDISEEAIRALVDRLERESRNGTETVKQHPIVKNGRSRWDHGLIEEHIP
                     RGASVLDLGCGDGSLLARLMKTKGIHAQGIEIDFSLVLDCVTNGVPVFQANLDEGLAG
                     FPDRSFDYVVLEETIQTLNRPRTVLSEMVRVGKLGIVTFPNFGYWQVRLDLALTGRMP
                     VTKSLPFAWFDTPNIRLLTLEDFRQWTADEGIEIVEGHVFSEGTIRRIQDDDNLFADE
                     VMLVIRKAV"
     gene            2457..>2690
                     /locus_tag="ENN56_00625"
     CDS             2457..>2690
                     /locus_tag="ENN56_00625"
                     /inference="COORDINATES: protein motif:HMM:PF01522.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74020.1"
                     /translation="MFRRYFQSDCDIHLLIPNDRTHLVADQPSFPWPNGERAAVSLTF
                     DDGVASQYETVIPMLNKRGFHGTVYIPMAEGGRF"
BASE COUNT          563 a          791 c          806 g          530 t
ORIGIN      
        1 ccttgtcggt tgggtgaact accccggcct gaagagtcat ccggataacg agcgcgcgaa
       61 gaagtatcta ccgaatggtt ttggcgcgat catcggtttc ggtatcaagg gcggacggac
      121 cgccgggaag aagttcatcg agagcgtgaa gctcttctcg catctggcga atatcggcga
      181 tgcgaagagt ctcgtgattc atccggcgac aacgacgcac cagcaactca ctccggaaga
      241 acaagcatcg tccggagtca ccgacgacta cattcgtctg tcgatcggtt tggaaaacat
      301 cgaagacatc atcgccgatt tggatcaagc gctcgccgcg agtcaggcat aaccgtccgg
      361 cggtccgagg cgtctcgctt cggaccgctc gcacggcacg acggcggcac aaccggatcg
      421 gtctacggag acggcatgct cctaatacaa gacgaggacg cggtacgaaa actcttcccg
      481 ccgggcgcgc tcgaagcggt gaattcggtg ggagcgacgc gtccgcaccg cgtgcgcctc
      541 gttgaagaga acgactcgct cccgctcacg gtcggcggca aaatcgcgcc ggtcgacgtc
      601 gagtatgaga tgtacggcac gctcaatgcc gacaaatcga acgtcgtcgt tatctttcac
      661 gcgctctccg gcgatgcaca cgttgcgggt tgggatatga gcgccgtaga gaccggtcgg
      721 cggtggcgct tgcgcaagcc cggctggtgg gacgaaatcg tcggtccgaa taaagcaatc
      781 gacaccgagc gttggtgcgt gatctgtgtg aatgcgcttg gcagctgcta cggcacgacc
      841 ggaccgtcat cgatcaatcc cgcaacgggc aaaccctatg ctctgtcctt ccctattgtc
      901 accgtcgaag actgggtgcg cctgcaggtg atgctgctcg atcacctcgg catcgagcgg
      961 gtgcatgcag tgattggtgg ttcgctcgga gggcaacagg tcatcgagtt catgatggcg
     1021 ttccccgacc gcaccgagaa ggcgatcgtg ctcgccgcgt cgtcgaagct gagcgcacag
     1081 ggactcgcat tcaacgctgt cggtcgccat acgattatga acgatccgaa tttctccgac
     1141 ggcgactatt acgatcgcga tgtccccggt caggggctcg ccgcggcacg gatgcttgcg
     1201 cacatcacgt atctcagcga aatcggcatg gaaacgaagt tcgggcgacg actccagaac
     1261 aagaccgaac ctgacttcga tttcggtatt gaattcgagg tggaaagtta tctcgcgtac
     1321 caaggccgct cattcgtgga gcggttcgat gcgaacagct acctctacat cacacgcgcg
     1381 atggactatt acgacgccgc ggcaactcac ggcgggggga atctcaccga ggcgtgttcg
     1441 cgcgtcacgg ccgagagtat ggtcgtttcg ttctcgtcgg attggctcta tccgcccaat
     1501 caatgccggg agttggcgat cgcactctcg cagttccatc ggccggtgac atatatcgat
     1561 gtgccgtcgt cgtatggcca cgacgcgttc ctcgtcgaga cggagatcgt cggcaggctt
     1621 gtcagcgatt tcctcggtcg ggaggttgcc gatggctgaa cgcgacgcaa tcgaacggct
     1681 cgtcgatctc cttaccgccc gcaccgagat taccggtcgc gatatcagcg aggaagcgat
     1741 acgcgcgttg gtggaccggc tcgaacgcga gtcgcgaaac ggtaccgaga cagtgaaaca
     1801 gcatccgatc gtgaaaaacg ggcgttcccg gtgggatcac ggactgatcg aggagcatat
     1861 cccacgtggc gcatcggtgc tcgacctcgg ctgcggcgac ggttcgcttc tcgcacggct
     1921 catgaagacg aaaggcatcc acgcgcaggg catcgagatc gacttcagcc tcgtgctcga
     1981 ctgcgtcacg aacggcgtgc ccgtatttca ggcgaacctc gacgagggac tcgccggatt
     2041 ccccgatcgc agcttcgatt acgtcgtgct cgaagagacg attcaaacac tcaatcgtcc
     2101 gcgtacggtg ctgagcgaga tggtccgcgt cgggaaactc ggcatcgtga cattcccgaa
     2161 cttcggctac tggcaggtcc gactcgatct cgcgttgacg ggccggatgc cggtcacgaa
     2221 gtcgctcccg ttcgcatggt tcgacacgcc gaatatccgc ctgctcacgc ttgaagattt
     2281 ccggcagtgg acggccgacg agggaatcga gatcgtcgaa ggacacgtct tttccgaggg
     2341 cacaattcgg cggatacagg acgacgataa cctcttcgcc gacgaagtga tgctcgtgat
     2401 tcgcaaagcg gtgtaagttt tcccattcac gccaagaaaa ctcattggta cgctgagtgt
     2461 tccgcaggta ctttcagagc gattgtgaca tccatctcct cataccgaac gacaggacac
     2521 acctcgtggc tgatcaaccg agtttcccat ggccgaatgg cgaacgtgcg gccgtatcac
     2581 tcacgtttga cgacggcgtc gcctcgcagt acgaaaccgt catcccaatg ctcaacaagc
     2641 gtggattcca cggcaccgtc tatatcccca tggctgaagg cggtcggttc
//
LOCUS       DSCC01000056            2835 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024150, whole genome shotgun sequence.
ACCESSION   DSCC01000056 DSCC01000000
VERSION     DSCC01000056.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024150"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            63..863
                     /locus_tag="ENN56_00630"
     CDS             63..863
                     /locus_tag="ENN56_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74021.1"
                     /translation="MTKKRTHRVLITAAAIGMALMVSGCYTVLTHPTTSPNQHVTDSG
                     EVIAQDNCAGCHSGGELWSYHHNPYWLAPSHQHSLSGYYAYRYDHLFLGTRYYDPYYY
                     DRWASYYYRPWWGRQRYPRYHPTPSVPINANDDPGRGALPTGAYTQELRDEYIPLLGP
                     GSGPMSFSRPLQPVATNGDQPAARTGQDETDSESKSVSTRSDAGRGAPPTSVTPVQRR
                     VPPTQSSPPPSSSSSGSAQKQSTQSSDDDDSGDDGRGSTPSGAEPRRR"
     gene            890..2245
                     /locus_tag="ENN56_00635"
     CDS             890..2245
                     /locus_tag="ENN56_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74022.1"
                     /translation="MKRFLQMSVGIAALFIAANVNAQSSRTPELLNVLAINRGVGELV
                     PSGFVGVGARAMGMGGAQIAVVNDGSALYYNPAMLVHIPRVELSGGISLIRSNPSTTF
                     GAINPTDASASASYTRLNNLLVTVPYPVYRGGFTIAFGATRPTDFAYRSQREGELSHL
                     GKSFVLDDRIRQEGGISQYAIGFGVQVSELVSLGAAGSWYHGGADITRDVSLVQQNPA
                     VGALPDEYYGAYDETQRLTGFMFSVGTSVRLPHDLTLGVTVTPPFTYSIKTTWGQEYE
                     EYEGDHVYPYPYSENSFSYGVRRPWKFGIGAGWTTYALTLAGDVWYEDWTQARYRDRS
                     PFDSDASVDAATYFEDRYRSTVRWHLGAEYLLPILKTKARVGYYSAPDPFIGPVLATN
                     ETIRYVDEAGYFTFGLGWLIDNVVAFDVAYVTGGDKYRSGSLVENRNSRQILVTTAFR
                     F"
     gene            2547..2765
                     /locus_tag="ENN56_00640"
     CDS             2547..2765
                     /locus_tag="ENN56_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004162694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HDS74023.1"
                     /translation="MKISYDTEIDALYIRLLDGVHECRTVRLNEEIALNIGDGEKLVG
                     IEILDAARVLGSGRLPQVVLENIPIASA"
BASE COUNT          612 a          759 c          859 g          605 t
ORIGIN      
        1 gaattcactg atagagtcca ttgagataga tcgtcgttcg ccaccggact ggaggtgtgg
       61 ttatgacaaa gaaacgcacc caccgggtac tcattacagc cgcggcaatc ggcatggcgc
      121 tgatggtatc cggctgttac acggtattga cgcatccgac aacgtcgccg aatcaacacg
      181 tgacggattc cggtgaggtg atcgctcagg acaactgcgc gggatgccac tccggcggag
      241 aactctggtc gtaccatcat aacccgtact ggctcgcgcc cagtcatcaa catagcctct
      301 ccggttacta cgcgtatcgc tacgaccatc tgttcctcgg tacacggtat tatgacccgt
      361 attactacga tcgctgggcg agttactact atcgcccgtg gtggggacgg cagcggtatc
      421 cgcggtacca tccgacaccg agtgtgccga ttaacgccaa tgacgacccg ggtcgcggcg
      481 cacttccgac cggtgcgtat acacaggaac tgcgggacga gtatatcccg ttgcttgggc
      541 cgggatctgg tccgatgagc ttttcgcgtc cgctccagcc ggtcgccaca aacggcgatc
      601 aaccggctgc tcgaacggga caagacgaaa ccgattccga atcaaagagc gtatcgacgc
      661 gttccgatgc tggtcgcggc gcacccccaa cgagcgtgac accggtgcaa cggagagtgc
      721 cgccgacgca gtcgtctcct cctccgtctt ccagttcgtc cggatcggct cagaagcaga
      781 gcacgcagtc gagtgatgac gacgattcgg gtgacgacgg acgcggaagc acgccgagcg
      841 gcgccgagcc gcgtcgacgc taagactcga tgtatcggaa ggtggcggta tgaagcggtt
      901 tctccagatg agcgtaggca ttgctgcgtt atttatcgcg gctaacgtca acgctcagtc
      961 gagtcgaact cccgagcttc tcaacgttct cgcgatcaac cgtggcgtgg gtgaacttgt
     1021 gccgagtggt ttcgtcggtg tcggtgcaag ggcgatggga atgggtggcg cgcagatcgc
     1081 cgtcgtgaat gacgggtccg ccttgtacta caatcccgct atgctcgtcc acattccgcg
     1141 cgtcgagctt tccggtggca tttcgctcat tcgcagtaat ccctcgacga cgttcggcgc
     1201 catcaacccg accgacgcga gcgcgtcggc aagctatacg cgactcaata acctactggt
     1261 gacagtgccg tatccggtgt accgcggcgg tttcacgatc gcgtttggtg cgacgcgtcc
     1321 gacggacttc gcgtatcggt cgcaacgcga aggtgagttg tcgcatctcg gcaaatcgtt
     1381 cgtactcgat gatcgtatcc gacaagaagg cggtatatca cagtacgcca tcgggttcgg
     1441 cgtacaggtg tccgaattgg tatcgctcgg tgcggcgggt tcatggtatc acggcggcgc
     1501 cgacattacg cgtgacgttt cacttgtcca gcagaatccg gcagtcggcg ctcttcccga
     1561 tgagtactac ggagcgtacg acgaaaccca aaggcttaca ggattcatgt tcagcgtcgg
     1621 tacatcggta cgactgccgc acgatttgac attgggtgtt accgtgacac caccgtttac
     1681 ctactcgatc aagaccacgt ggggacagga atacgaggag tacgaaggtg accacgtgta
     1741 tccgtacccc tacagcgaga acagcttctc gtatggggtt cggcggccat ggaagttcgg
     1801 catcggcgcg ggatggacaa cgtacgcgtt gacactggcg ggcgatgtct ggtatgagga
     1861 ctggacgcag gcgcgatatc gtgatcggag tccgtttgat tccgatgcgt cggtggatgc
     1921 ggcgacgtac ttcgaagatc ggtatcgttc gacagtgcgc tggcatcttg gggcggagta
     1981 cctcctgccg attctgaaaa cgaaggcgcg cgttggctac tactcagcgc cggatccatt
     2041 cattggaccg gtactcgcga cgaacgagac gattcgctac gtcgatgagg cgggatattt
     2101 cacgtttggt ctcggttggc tgatcgataa cgtcgtggcg ttcgatgttg catacgtgac
     2161 gggtggcgac aaataccgct cgggaagcct tgtagagaac cggaacagtc ggcagatact
     2221 cgttacgact gcgttccgat tctgatacag tacagcggac gccaaggtta gggtatattc
     2281 tgaaaccgat ccggttcacg cggcacgcag gtgagcaatg taacgaccgt ggcgcgaccg
     2341 aggacgaagg tatttgtgcg gttagagaag gaacgtgtga accggcgcga cttggtcggg
     2401 agttgcgtag gtacaacttt ccattcggac ggatatggca aggaaaacac cacgcaatca
     2461 aacaggtagc accagtgatt gtagaagagg caaaagaaat cgttattata accgtctata
     2521 cgttttattt ttgaatcgag gtcgcgatga aaatcagcta cgataccgaa atcgacgcgc
     2581 tttatatccg cctgctcgac ggtgtacacg aatgccggac ggttcgtctc aacgaggaga
     2641 tcgcgctgaa tatcggtgat ggagagaagc ttgtcggtat agagattctt gacgctgcgc
     2701 gggtactcgg ttccggtaga ttgccacagg tcgttttgga gaacataccg atcgcgagcg
     2761 cataagacca acactcgtca gcggagtttt atcgcagggg tagggacgca cggcagtgcg
     2821 tccctacgtt tgtac
//
LOCUS       DSCC01000057            2064 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036903, whole genome shotgun sequence.
ACCESSION   DSCC01000057 DSCC01000000
VERSION     DSCC01000057.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036903"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            36..782
                     /locus_tag="ENN56_00645"
     CDS             36..782
                     /locus_tag="ENN56_00645"
                     /inference="COORDINATES: protein motif:HMM:PF12697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS74024.1"
                     /translation="MAAERCVEITRKDGTRVTGRIREGDGPTLVLIPGTWGNLSTRGP
                     LMKRLDSSLRIVNVALPGQDDNWPPPEHPSIPEFSEDVMRLADVIGIKQFVVGGNSLG
                     GMISVDMLRFAPDRVIGAIPIEGWTRSSVSKEAFGDNVQGCLTEKQREFLEEVRHELL
                     DRWDPELRRRYSSVWHQWDGWDILNASEVPVLEIWGDRGQPRPSRDVMAVPDRPNIEL
                     QWIAGCSHSLLVEAPETLAELINKFMREFA"
     gene            complement(790..>2064)
                     /locus_tag="ENN56_00650"
     CDS             complement(790..>2064)
                     /locus_tag="ENN56_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDS74025.1"
                     /translation="YMPPTPRKPVTHTRNNAARLPELLPGVPRRREVRPHIGQPLGLL
                     VKPASADCNLNCEYCFYHERASDPYKGAGDGHRGAMHRMSNETLDAFLSQWLPLAGPN
                     PNVGWQGGEPTLAGVDFFRQVVKRQQELKDPRQTVSNALQTNGVLIDDEWAEFLHEHR
                     FLVGLSFDGPQELHDRYRFDYAGKGTFGRIMRSIEAMQKHDVEFNLLCVVNRLTANHP
                     EKIYEFFTKQGFQWLQFIPAVERDAKGRVTSFSVTPKQFGTFLCRMFDCWYQDGKPTV
                     SVRLFDELLGIGMGQPPGSCQLNAVCGGLYLVVEYNGDIYPCDFFVEDRWKLGNVHEG
                     TMTEMLQSERLKEFAQVKPNASSKCETCRFQGICHNGCPHYRSLGDGKFLELDYLCEA
                     YYRFYSHAIPKMQKLLHELERTGGGRHPQMMH"
BASE COUNT          451 a          568 c          584 g          461 t
ORIGIN      
        1 accatagggc gtttaccgta acggagagtc gtaacatggc ggccgaacga tgtgtagaga
       61 tcacgcgaaa ggacggaacg cgggttaccg gtcggatccg tgaaggtgac ggaccgacgc
      121 ttgtattgat tcccggtacg tggggaaatc tctctacgcg tggaccgctg atgaagcgtc
      181 tcgattcgtc gctacgcatc gtcaacgtgg cgttacccgg acaagacgac aactggccgc
      241 caccggaaca cccctcgata cctgagttca gcgaagacgt gatgcgtttg gccgatgtaa
      301 tcggtatcaa acagttcgtc gtcggcggaa acagcctcgg tggaatgatc tcagtcgata
      361 tgctacggtt tgcgccggac cgagtaatcg gcgcgatccc gattgaaggg tggacgcgat
      421 cttccgtttc gaaagaggcg ttcggcgaca acgtccaagg ttgtctgacc gaaaaacagc
      481 gtgagttcct cgaagaggtt cgtcacgaac tgctcgatcg atgggatcct gagttgcgac
      541 gacggtattc ctcggtatgg catcagtggg atggctggga tattctaaac gcgagtgaag
      601 taccggttct cgaaatctgg ggagaccgcg gccagccacg cccgtctcgc gacgtgatgg
      661 cagttccgga caggccgaat atcgagttgc aatggatcgc tggatgctcg cactcgcttt
      721 tggtcgaagc tccggaaacg ctcgcagaac tgatcaacaa gtttatgcgc gaatttgcgt
      781 gacagacgat cagtgcatca tctgtggatg tctaccgcca cccgttcgtt ccaattcgtg
      841 gaggagcttc tgcattttgg ggatagcgtg actataaaac cgataatacg cctcgcagag
      901 gtagtcgagc tcgaggaatt taccatcgcc gaggctccga tagtgcgggc aaccgttatg
      961 gcaaatcccc tggaatcggc atgtttcgca tttactcgac gcgttcggtt ttacctgcgc
     1021 gaattccttc agtcgctcgc tctgaagcat ctcggtcatg gtgccttcgt gaacgttccc
     1081 gagtttccat cgatcctcga caaagaaatc gcagggatag atgtcgccgt tgtattcgac
     1141 taccaaataa agcccgccac ataccgcgtt caactgacat gaaccgggcg gttgtcccat
     1201 tccgataccc agcagttcgt cgaagagccg aacactcacc gttggcttcc catcctgata
     1261 ccagcagtcg aacatccggc agagaaacgt gccgaactgt tttggtgtca cgctgaacga
     1321 tgtcacacgc cccttcgcgt cgcgctcaac agccggaatg aactgcaacc actggaatcc
     1381 ttgcttcgta aaaaactcgt agatcttttc gggatggttc gccgtgagcc gattcacgac
     1441 gcaaagcagg ttgaactcta cgtcatgctt ttgcatcgcc tcgatcgacc gcatgatacg
     1501 tccaaacgtg ccctttccag cgtagtcgaa ccggtaccga tcgtgaagct cctgcggtcc
     1561 atcgaagctc aagccgacga ggaaccggtg ttcatggagg aactccgccc attcatcgtc
     1621 gatgagaacg ccgttcgttt gaagcgcgtt cgagacggtt tgacgcgggt cttttaactc
     1681 ctgttgccgt ttcaccacct gcctgaagaa atcaacgccc gcgagcgtag gctcgccacc
     1741 ttgccaccca acattcggat tcggtcccgc cagtggcagc cactgcgaga gaaacgcgtc
     1801 cagcgtttcg ttcgacattc gatgcattgc gccgcggtgg ccatctccag cgcctttata
     1861 ggggtcgctc gcacgttcgt gatagaaaca gtattcgcaa ttgagattac aatccgcgct
     1921 tgccggcttt acgagcaatc cgagcggttg gccaatgtgc ggtcggactt cgcgccgacg
     1981 gggaacgccg gggagaagtt cgggcaatcg tgctgcgttg ttgcgcgtgt gagtgacagg
     2041 tttccgcggc gtaggaggca tata
//
LOCUS       DSCC01000058            2918 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023215, whole genome shotgun sequence.
ACCESSION   DSCC01000058 DSCC01000000
VERSION     DSCC01000058.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023215"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            277..504
                     /locus_tag="ENN56_00655"
     CDS             277..504
                     /locus_tag="ENN56_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74026.1"
                     /translation="MMYTIYAIVVFIMGKTIPGWLSIVGVSLFWGGVLLIMQGVTAEY
                     VGRIYSEIKRRPLYFVSSAYGIDTNKPSETP"
     gene            591..2096
                     /locus_tag="ENN56_00660"
     CDS             591..2096
                     /locus_tag="ENN56_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HDS74027.1"
                     /translation="MPHPKRPHIVIFNPDQWRGDTLRHVGNPAAVTPNLDRVAATDGV
                     SFSRAFCQATVCTPSRASFMSGWYPHVRGHRTMYHMLHQYGNVTDEPILLQMLKDSGY
                     YVWWGGKNDLTPGQEPTSRCCHVRNRIPPRPPELGQRVNEHDHIQAERGHPGSDTYYS
                     FYTGKIELPEAERPYDHDWGHILAACEMIRSADIDRPLCVYLPIGFPHPAYRVEEPFY
                     SMIDRNAIPPRIPTPPGPWEGKPSMLRGIWQRQNMQSWSEDRWTELRATYYGQCARVD
                     YQFGLVVDALKQAGIYDDTAIFFFSDHGDFTGDYGLVEKNQNTFEDCLSRIPFVVKPP
                     AWVDVKPRVSDALVELIDMKATVEDLCGIEPDHTHFGRSLIPVLTGETTEHRDAVFAE
                     GGRREGETHCMELESSSSQAPEGEYWPRVNLQQQLPEHTKAIMCRTHTHKYVRRLYEH
                     DELYDLERDPAELHNVIEDPAYANVLASLKERLLTHYLDTSDVVPHRTDRR"
     gene            2240..>2918
                     /locus_tag="ENN56_00665"
     CDS             2240..>2918
                     /locus_tag="ENN56_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74028.1"
                     /translation="MIRRFKRIVGGLTVALLVVVSGASAYEPFLQTPRWRTLDDLTEL
                     PNLYVNDIVSTIGGEMWIATDRSIGTHDGTVYVSRDVDFLPQDVRYNTLLYDVLGTIW
                     AGTSSGLVRYVGHQWRLMLDSGDVTAITESVDGTVWIGVSGEFDAGAIQSGIEPGIYR
                     YQSGAWTRYGASDGLPTGRVQCLYGDANGGVWAVYGTETRMDALFRFEGARWRDMVRV
                     LNPPSGAI"
BASE COUNT          643 a          875 c          805 g          595 t
ORIGIN      
        1 atactcagag gtggagttga ttcccgcctg cttgaccgaa aagctgcgga tgcattacgg
       61 caactgcgag aacgagaccg ttttgttcgt ggtctcgtac cgtggattgg attccgacag
      121 acggcagtag agtatcaagc cgctcctcgg ttgtctggcg agtcgaaata cccatttcga
      181 cggatgcttc gattcgctgt tgacggcgtt gtttcctttt ccgcaacccc gttgagaatc
      241 gggctttata ttggcgtcgt gtcggcgata atcgcgatga tgtacacgat ctacgccatc
      301 gttgtattca taatggggaa aacgattcca ggatggttgt ctatcgtcgg agtaagcctg
      361 ttctggggtg gcgttttatt gatcatgcaa ggtgtaacag ctgaatacgt cggaagaatc
      421 tattcggaga tcaaacgtcg tccactgtat ttcgtttcat cggcgtacgg tatcgataca
      481 aacaaaccaa gcgagacgcc gtagaacacc atacatcttc cccttgcccc gacaactcca
      541 cacgcacact atcgactccg taccgctact cccaccgccg gatttcaacc atgccgcatc
      601 caaaacgacc gcatatcgtc atcttcaacc cggaccagtg gcgcggcgat actctgcgtc
      661 acgtcggaaa tcccgccgcg gtaacgccga acctcgaccg cgtcgccgct accgacggcg
      721 tctcattcag ccgcgcgttc tgtcaagcga ccgtatgcac accaagccgc gcgagcttca
      781 tgtcgggatg gtatccgcat gtgcgcgggc accgaacaat gtaccatatg ctccatcaat
      841 atggaaacgt caccgacgaa cccattttgc tccaaatgct caaggattcc ggctactacg
      901 tctggtgggg cggaaagaac gacctcacac ccgggcagga gccgaccagc cgctgctgcc
      961 acgttcgtaa ccgcattcct cctcggccac cagagctcgg acaacgtgta aacgaacacg
     1021 accacatcca agcggaacgc ggacaccccg gcagcgatac ctattactcg ttctacacgg
     1081 gcaagatcga gctccccgaa gccgaacgcc catacgatca cgattgggga cacatactcg
     1141 cggcgtgcga aatgattcgc agcgcggata tcgaccggcc gctctgcgtg tacctaccaa
     1201 tcggtttccc acatccggcg tatcgcgtcg aagaaccgtt ctacagcatg atcgaccgaa
     1261 acgccatccc gccgcgcatc ccaacgccgc ccggaccatg ggaggggaaa ccgagcatgc
     1321 tccgcggtat ctggcagcgg cagaacatgc agtcgtggag tgaagaccgc tggaccgaac
     1381 tacgcgcgac gtattacggt caatgcgcgc gcgtcgacta ccaattcggg ctcgtcgtcg
     1441 acgcgctcaa gcaggccggc atctacgacg acacggcgat cttcttcttc agcgaccacg
     1501 gcgacttcac cggcgactac ggtcttgtcg agaagaatca gaatacattc gaagactgcc
     1561 tcagtcgcat tccgttcgtc gtgaaaccgc cggcgtgggt cgatgtcaaa ccacgtgtaa
     1621 gcgatgcctt ggtcgaactc atcgatatga aagcgacggt cgaggacctc tgcggtatcg
     1681 agcccgacca tacgcacttc ggacgctcgt tgattcccgt gctcaccggt gagacgacgg
     1741 agcaccgaga cgccgtgttc gctgagggcg gtcgacgcga aggcgagacg cactgcatgg
     1801 aacttgaatc gtcgagctct caggcgccgg aaggtgaata ctggccacgg gtcaacttac
     1861 aacagcagct tcccgagcat acgaaagcga ttatgtgccg gacccacacc cacaaatacg
     1921 tgcgccgact ctacgaacac gacgaattgt atgacctcga gcgcgatccg gcggaacttc
     1981 acaacgtaat tgaggatccc gcatacgcta acgtgcttgc gtcgttgaaa gaacgtctcc
     2041 taacacatta tctcgatacg tctgatgtcg taccgcaccg caccgatcgt aggtagagcg
     2101 caccgcacac tccgacggaa aagcgatcgg cgttgccgca aacgacggcg tgccgtatga
     2161 tacgtatcgg ttcccattaa tcgcgaaatt cacggcattc gcacgattcg gtgctgttcg
     2221 ttaccacgga gcattggcga tgatccggcg attcaaaaga atcgtaggcg gtctgaccgt
     2281 cgcgttgctc gttgtggtat ccggagcgag cgcgtacgaa ccgttccttc agactccgcg
     2341 ttggcggacc ctcgatgacc tcaccgaact accaaacctc tatgtgaatg atatcgtttc
     2401 gacgattggc ggcgagatgt ggatcgccac cgaccgctcg atcggcactc acgacggcac
     2461 agtctacgtc tcgcgcgatg tcgacttcct tccgcaggat gtacggtaca acacgctgct
     2521 ctacgatgtt ctcggcacga tatgggccgg gacatcatcg ggcttggtgc gctacgtcgg
     2581 gcatcaatgg cgcctcatgc tcgatagtgg cgacgttacg gcgattaccg aatctgtcga
     2641 tggaaccgtg tggatcggcg tctcgggcga attcgacgct ggggcgattc agtcgggtat
     2701 cgaaccgggg atctaccggt atcaatccgg cgcatggacg cgttacgggg cttccgacgg
     2761 gctccctacg ggacgtgttc aatgcctgta cggtgacgcg aacggcggag tgtgggctgt
     2821 ctacggcact gagacccgca tggatgcgct cttccggttc gaaggcgcgc gatggcgcga
     2881 catggttcgc gtattgaacc caccttccgg cgccatac
//
LOCUS       DSCC01000059            1794 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044643, whole genome shotgun sequence.
ACCESSION   DSCC01000059 DSCC01000000
VERSION     DSCC01000059.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044643"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1118
                     /locus_tag="ENN56_00670"
     CDS             <1..1118
                     /locus_tag="ENN56_00670"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00324.19,HMM:PF03522.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS74029.1"
                     /translation="NLATFYESITKNPSYRPRFRFSHWTTELLGALACVVVMFLISAT
                     WASITVLTISALYWYIARKQIIARWGDVKHGIAFERTRKNLLRLEDEEYHPKNWRPMI
                     LALSGGAWSRLYLAVYGHWLAGGNGVLTLAQIIVGDVRQLLERRRNQERLLSRFISEE
                     ELAAFPAVVVSPSIEQGIQTLVQAAGIGAVRPNTVLIGWTRDPSRIETFGTTLRTIAG
                     LGRSIVVVKTGELDKEHAWEAQPGTIDVWWRGRVNGTLMVLLAHLLVQNNEWRGRMIR
                     LIRAIPSEAGREEAEKHLNRLIELARIRAQSVVVIADDVTTAIHEVSASAAVTMLGFT
                     PPEPGHEESFIEAMNRFTDGLGTTILVSSAGGMDLEA"
BASE COUNT          376 a          507 c          529 g          382 t
ORIGIN      
        1 tcaaccttgc gacgttctac gaatcaatca cgaagaaccc gagctaccga ccacgatttc
       61 ggttcagcca ctggacgacg gagttactcg gagcgctcgc gtgtgtagtc gtcatgtttc
      121 tcatttcggc gacgtgggcg tccatcacgg ttctaacgat cagcgcgctc tactggtaca
      181 tcgcgcgaaa acagattatc gcacgctggg gagatgtcaa acacgggatc gcgttcgagc
      241 gcacgcggaa aaacctcctc cgactcgaag atgaagagta ccatccgaaa aattggcgcc
      301 cgatgatcct cgcgctctca ggtggtgcgt ggagccgttt atacctcgcg gtttacggtc
      361 attggcttgc aggtggaaac ggtgtactga cgctcgcgca gatcatcgtt ggcgatgtcc
      421 gccaattgct tgaacgacgc cgcaatcagg agcggctgtt gagtcggttc attagcgaag
      481 aagaactcgc cgcgtttccc gcagtcgtgg tgtcaccaag catagagcaa ggcatccaga
      541 cactcgtgca ggcagccggt atcggcgcgg tccggccgaa taccgtactc atcggttgga
      601 cccgagatcc cagccgtatc gagacattcg gaacgacact tcgaaccatc gccggactcg
      661 ggcgaagtat cgtcgtggtt aaaacgggcg agctcgataa ggaacacgcg tgggaggcgc
      721 aaccggggac aatcgatgtc tggtggcgtg gacgcgtgaa tgggacgctc atggtcctcc
      781 ttgcgcactt gctcgtgcaa aacaacgaat ggcgtgggcg aatgatacga ttgattcgag
      841 cgataccctc agaagccggt cgcgaagaag cggaaaaaca cctgaatcgg ctcatcgagc
      901 ttgctcggat tcgtgcgcaa tcggttgtcg tcattgcgga tgacgtaaca acggcgattc
      961 atgaggtgag tgcgagcgcg gcggttacga tgcttgggtt cacccctccg gaaccggggc
     1021 acgaggagtc attcatcgaa gcgatgaacc gattcactga tggtctcggc accacgattc
     1081 tcgtttcgag tgcgggcgga atggatctcg aagcatagag cgaacgtcag gcgtggaggt
     1141 ctcgcacgaa cacgcgttga tcgttcgcac tccgaaggtc ttccgcgatc tcacgcaagc
     1201 gcatgtgatg cgtgaagaag agcacctgaa cgcggttgga aaggtccgca agcacttcga
     1261 gcgccgcacg tgcgcggtcg tcgtcgaact tgatgaggat atcgtcgacg atgaacggta
     1321 gcggcttgtt gcgggagaca tagtgctcca acgcggcaag tcgtagcgag agatagagct
     1381 gatcggcggt tccctcactc atccctccga tcgctacgag actctgctcc gcgtcgccgc
     1441 gtatccccat gagtaagggt tcatccttct cgttatactc ggtcttcagt cccgcgaacg
     1501 aaccgagtgt caaacgaccg aaaaatccgc tcgctgcctt cagaatcggc ccctggtttt
     1561 cggcacggta tttttcgatc tcgttgctga ggatgcggtg cgcgagtttg acccgcacgt
     1621 agcggtcgac atcggtgcga atctccgcac gtatgctcgc cgcttcatca gccgccatag
     1681 cagcctcgtc gctgccgtcc atccggtctc gctcgttctc ctttgcaccg atcgtgcgct
     1741 tgagttcgtc cagttcctgt gtgagctccg tctccttcga cgaaagcgct tcga
//
LOCUS       DSCC01000060            5860 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1008939, whole genome shotgun sequence.
ACCESSION   DSCC01000060 DSCC01000000
VERSION     DSCC01000060.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1008939"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..480)
                     /locus_tag="ENN56_00675"
     CDS             complement(<1..480)
                     /locus_tag="ENN56_00675"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS74030.1"
                     /translation="MAVEAESHGYDASVIIPHYAGSQVFDCIESVLSCPDRPVEVILI
                     DDASIDDTAYEASERFPSIRVARNRSNLGFVGACNRGIAEATTRYVVLLNDDAIVEPG
                     WLAECIDALDSDPELAAVQPKIVQASDPTRFEYAGAAGGLIDRFGYPYALGRWFDNVE
                     "
     gene            complement(493..3849)
                     /locus_tag="ENN56_00680"
     CDS             complement(493..3849)
                     /locus_tag="ENN56_00680"
                     /inference="COORDINATES: protein motif:HMM:PF11028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HDS74031.1"
                     /translation="MSESRVTKRIGVLVWFGVFAVYFATMAPTTSFWDCGEFIAAAFT
                     LGVPHPPGSPLYNLLGRIFSLFPIEVPLKAIGLLPDFADMAVRVNIMSPIAGAFSALF
                     AYLIGLRLIRGWSGALGRDRNDWTAQVGAVTGALIFAFADSNWFNAVEAEVYAYAIFL
                     MMLVLWLGLKWADSVGEPKHLSYALAIAYVVGLAGGLHMLCLLVLPSIGILALFTYVK
                     DRRDTWVLLIAAALLAIGGYFGLHFINAIGLDEGPMSAPAYTFGIAETRDSYITGSLV
                     LALIPGLAGLFLTFRTKTVPVRDTWTVILIGAAAGATIKAVMGVFYWYQGVESFPTAM
                     IGLGIASGVGLLFFSNAANKPRIPILKSYHLSVALLVLVVLGYSTYLTLMIRSGLNPI
                     IDENNPENWRNFFDFVARKQYGTESMSLTIFERRANFGWQFWDMLFKYLFQQFPASIT
                     GTLFDWKIFFRAATEPIYFGMRVPDLPLILMFFGLVWQYESDKKRFLALVSLWTIGGI
                     GLAVYLNMPDPQPRERHYVFTAAASVMALWMGMGVTGLIRAIPTWIPNSVSAAIRNRA
                     LPITAAVIGLFVPVWFLIGYPLVDEYSTDRSVRYDNWTKQNRHLDTVAYDYAYNILQS
                     CDPNGILFTNGDNDTFPLWYLQEVVGVRRDVRVVNLSLLNTDWYIQQLRDNEPKLPIS
                     SRYTDDFIVNVLTGSSLEALVRSNRIDVADGSPFNREGRLVGWQTKEVTAAGVDTARI
                     TLTNGEVIRGVVRDTDASEISVMPHDTRLWQSIDRNEIADIDVGRAELRWTLAAERDF
                     PVLRVQDVMVYNLIHWVKWELPVYFAVTVSADNRIGLDPYLRMEGMVLKVEMEKDPSV
                     EPGSDMYGLNPERSRFNLDSVYVMRSLMDQTIYKDDNMQKLISNYRSAYLQLADTYLS
                     MNQPEEAAVTLQKMIDRLPMDWRSSYSAASIIARRDEPVLKKLGAHFAIHSATVLARH
                     IEQEDLFGSYMIQQTRMTARLLRYNAGDDSASMDLAADLLTSASERLRESPNATIMDQ
                     LALAFDAAMAYEDAGNLQRAYEVFSDVNAALSPLMDRSDVAEQLRTSLQIDPSRLLIE
                     VQRKLAELEEQIRLIHSFETPGDTSSGITN"
     gene            4268..>5860
                     /locus_tag="ENN56_00685"
     CDS             4268..>5860
                     /locus_tag="ENN56_00685"
                     /inference="COORDINATES: protein motif:HMM:PF13860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74032.1"
                     /translation="MSAFASVRSVSAQPSRAEGPFADASHAKLAAGRRGLESNEAAIF
                     AVSGDSLWVAAGTTISRRVGAGSSQFDWETYTVRIDTLHSGESITAFAAQGDRLAVST
                     SFGVQTSQGVYGAGSGLVLSADGGNTWVHHSFSDIFPDSSRQALQIPNGDVQCFGIWF
                     EGENLWSAWTSEFAVMTPDWGVTWNRYRPDSTNNPQPNPFLDDPTLQHRYLHLNYRAY
                     DGTMLGDTLWVGTNAGINRSVDGGVTWENYDELTVGISGDFVPAIAADSENGIVWAAT
                     QATGIDEAEIKQREIDYFNDGVFNELDYDLDRDGRLDGPGRNGISWTTDGGLTWQTYV
                     PETDPAFGKTFRAWNFAFDGTTVWVAGSVSGGDALLTSTDLGASWELQPIVTVDGDAV
                     RSASGTGDVAYFDGVLWATTGQGLARSDDRGQTWQFVLAFPQADPLDGGSVEDPDAAR
                     SNLETYAFPSPCSPISGTPPNIVFALTQPSDVTIDIYDAGSGLVRTIHRSSVSAGNHT
                     VRWDGRTESGRYVANGVYLYRIT"
BASE COUNT         1289 a         1571 c         1701 g         1299 t
ORIGIN      
        1 ctcgacgtta tcgaaccatc ggccaagagc gtaggggtat ccgaaacggt cgataagccc
       61 cccggcggcg ccggcatatt caaatcgcgt cgggtcggac gcttgtacga tttttggttg
      121 gactgcggcg agctctggat cagaatccag agcgtcaata cattcggcga gccatccggg
      181 ttcgacgatg gcgtcgtcgt tcagcagaac gacgtaccga gtcgtcgctt ctgcaatacc
      241 tcggttacac gctccgacga atccgaggtt gctccgattg cgtgcaacac gaatcgacgg
      301 aaatcgctcg gacgcctcat atgctgtgtc gtcgatactc gcgtcgtcga tgaggattac
      361 ttcgacaggt cggtcggggc atgacagaac cgattcgata cagtcgaata cttgcgagcc
      421 tgcgtagtgc ggaattatga cactcgcatc gtatccgtga ctttccgcct caaccgccat
      481 ggtccgcttc gctcagttcg ttatcccgct cgacgtgtcc cctggagtct caaaggagtg
      541 tatcaaccgg atctgttctt caagttcagc cagtttgcgt tgcacctcga tcaggaggcg
      601 acttgggtct atctgcaggg atgtacgtaa ctgttcggcc acgtcgcttc gatccataag
      661 aggggaaagc gctgcgttga cgtcgctaaa cacttcgtag gcacgttgta ggttcccagc
      721 gtcctcgtac gccatcgctg catcaaacgc gagcgcgagt tggtccatga ttgtcgcgtt
      781 tggactctcc cgcaaccttt cggacgcgct ggtcagcaag tctgctgcga ggtccatcga
      841 tgcggagtcg tctccggcgt tgtagcggag taatcgagcc gtcattcgcg tttgttgaat
      901 catatacgaa ccgaacaggt cctcctgctc gatatgccgt gcgaggactg ttgcggagtg
      961 gatggcgaag tgcgcgccaa gtttcttgag cacaggttcg tcgcggcgcg cgatgatact
     1021 cgctgcgctg taggacgatc gccagtccat cggaaggcga tcgatcattt tttgcagtgt
     1081 taccgccgcc tcttccggtt gattcatgct gagatacgtg tcggcaagtt ggagatacgc
     1141 agaccgatag ttcgagatca gcttctgcat gttgtcatct ttatagatcg tttggtccat
     1201 cagagaccgc atgacataca cgctatcgag attgaatcgg gaacgctccg gattcaggcc
     1261 gtacatatcc gacccgggtt caaccgatgg gtctttttcc atttcgacct tgagaaccat
     1321 tccttccata cgcaaatacg ggtcgagtcc gatacgattg tcggcggaaa ccgtaaccgc
     1381 gaagtacacc ggaagttccc attttaccca gtgtatgagg ttgtacacca tcacgtcttg
     1441 aactcggagt accgggaaat cgcgttcagc ggcgagcgtc catctaagtt cggctcgtcc
     1501 aacgtcaatg tctgcgatct cgtttcggtc tattgactgc cataagcggg tatcgtgtgg
     1561 cattacagag atctctgatg cgtcagtatc gcgtacgacg ccgcggatga cctcgccgtt
     1621 cgtgagcgtg attcgcgcgg tgtctactcc cgccgccgtt acttccttcg tctgccatcc
     1681 gaccaatcgt ccttcgcggt tgaatgggct gccatcggcg acatcgatgc gattcgagcg
     1741 gacgagtgct tcgagactgc tgccggtgag aacgttgacg ataaaatcgt ccgtgtagcg
     1801 actgctgatt gggagttttg gctcgttatc gcggagttgt tgtatgtacc agtcagtgtt
     1861 gagaaggctg aggttgacga cgcgaacgtc gcggcggacc ccgaccactt cctggaggta
     1921 ccacagcggg aacgtgtcgt tgtcgccatt cgtgaaaaga atgccgtttg gatcacacga
     1981 ttggaggata ttgtatgcgt agtcgtacgc aacggtatcg agatggcgat tctgtttcgt
     2041 ccagttgtca tatcgcaccg agcgatcagt agaatattcg tcgacgagtg ggtatccgat
     2101 gaggaaccac acgggtacga acaatccaat caccgccgcg gtgatcggga gagcgcggtt
     2161 ccgaattgca gccgaaacgg aatttggaat ccacgttgga attgcgcgaa tcaggcccgt
     2221 tacgcccata cccatccaca acgccatcac cgacgctgcg gcggtaaata cgtagtgtcg
     2281 ttcgcggggc tgcggatccg gcatattgag gtagacggcg aggccgattc caccgattgt
     2341 ccagagggag acaagcgcga gaaaccgctt tttgtccgac tcatactgcc aaacaaggcc
     2401 gaaaaacatg agaatgagcg gaagatccgg aactcgcata ccgaagtaga ttggctcggt
     2461 tgcagcacgg aagaaaatct tccaatcaaa gagtgtgccc gtgatcgatg cggggaactg
     2521 ctggaagagg tacttgaaga gcatgtccca gaactgccat ccgaagtttg ctcgacgttc
     2581 aaagatggtc agagacattg attccgtgcc gtattgtttc cgcgccacga aatcgaagaa
     2641 gtttctccag ttttccggat tgttctcgtc gatgatcgga ttcagaccgg accggatcat
     2701 caacgttagg tacgttgagt accccaacac gacgagaacg agcaacgcga cggatagatg
     2761 gtagcttttg agaatgggga tccgaggttt gttcgccgcg ttcgagaaga acaatagccc
     2821 gacgccgctt gctataccga gtcctatcat ggcggtcggg aagctttcga caccctgata
     2881 ccaatagaaa acgcccatga cggctttaat ggtcgcgcct gcagccgctc cgatgagtat
     2941 taccgtccac gtatcccgta ccggtacggt ctttgtacga aacgttagaa aaagaccggc
     3001 aagcccgggg atcagcgcga gtacgagaga tcctgttatg tatgagtccc tcgtctccgc
     3061 gattccgaac gtatatgcgg gtgcggacat cggaccttca tcgagaccaa tggcgttgat
     3121 gaagtggaga ccgaagtatc caccgatcgc gagcagcgcg gccgctatga gcaataccca
     3181 cgtgtcgcgg cgatccttta cgtaggtgaa cagcgcaaga atgccgatac tcggaagcac
     3241 gagcagacac aacatgtgca gaccgcctgc gagtccgacg acataggcaa tcgccagcgc
     3301 atacgacaga tgtttcggtt ctccgaccga atcagcccat ttcaatccga gccagagcac
     3361 gagcatcatg aggaagatcg cgtatgcgta gacctcggct tcgacggcat tgaaccagtt
     3421 cgaatcggcg aacgcgaaga tgagcgcacc ggtgaccgct ccaacttgcg cagtccaatc
     3481 gttacggtcc cgaccgagcg ctccgctcca gcctcgaatc agccgcagac cgatgaggta
     3541 ggcgaagagt gcggaaaacg cgccggcaat cggcgacatg atattgacac gcaccgccat
     3601 atcggcgaaa tcgggaagta gcccgatcgc tttcaacggg acttcgattg ggaagagtga
     3661 aaagatccta ccgagaaggt tgtaaagcgg agaccccggt ggatgaggga cgccgagggt
     3721 gaacgccgcg gcgatgaact cgccacaatc ccagaacgag gtcgtcggcg ccatggttgc
     3781 gaagtacacc gcaaaaacac cgaaccatac caggacgcca atacgcttcg taacccgtga
     3841 ttcactcata cgtaagcccc gtgtgtgccg tatccggttg ggaaacactc acgccgcgcc
     3901 ggtggaccga acggttcgcg tagcgtaggt aactccatag ttatacaagg tgtgtgagag
     3961 taccgaccgc gcgcgtgcga gtcaagtcag agaggcgttc acgggcggtt gtttgtctca
     4021 ctctgtgatt ccgttcgagc gttgtttgtt ccgtttacgg tttgtactcg cttgacgttt
     4081 tcgaccggta acgggatatt ccttatttcg tacctccgat gtctctcggg aaccgctatc
     4141 gacgtccgac ggttcaacgc cgagacggtg agcatcgatt cggaagtcat taggcgcgcc
     4201 cgttctacag gagtacagta tcaaatcgac aacctttctc tctcgcgtac tcatcgccgg
     4261 tatcgctttg agtgctttcg catccgttcg gagcgtctca gcgcagccga gccgagctga
     4321 agggccgttc gctgacgcgt cccatgcgaa gcttgccgct ggaaggcggg gcctcgaaag
     4381 caatgaggct gcaatcttcg cggtcagcgg agattcactc tgggtcgcag ccggaacgac
     4441 catcagtcga cgcgttggag ccggatcgtc gcagtttgat tgggaaacgt acacggttcg
     4501 aatcgataca cttcactccg gtgaatcgat tacggcgttc gcggcgcaag gtgaccgact
     4561 tgcggtgtcg acatcgtttg gggtgcagac cagtcagggc gtgtacggag ccgggagcgg
     4621 gttggtgcta tctgccgatg gcgggaacac atgggtgcat cattcgttct cggacatctt
     4681 tcccgattcg tcacgccaag ctctccagat tccgaacggt gacgtacagt gtttcggcat
     4741 atggttcgaa ggtgaaaacc tttggtcagc gtggacgagt gagttcgctg taatgactcc
     4801 ggattggggc gttacatgga accggtaccg tcccgattca acgaataatc cacaaccgaa
     4861 tcccttcctc gacgacccaa cgctacagca caggtacctt catctcaact atcgcgcgta
     4921 tgacggcacg atgcttggtg acacactttg ggtcggaacg aacgcgggta tcaatcggtc
     4981 ggtagatggt ggagtgacat gggaaaatta tgacgaactg accgttggta tcagcggtga
     5041 tttcgtgccg gctattgctg cggatagcga aaacggcatc gtgtgggccg caacacaggc
     5101 gaccggtatt gacgaagcag agatcaagca acgcgagatc gattacttca acgacggtgt
     5161 gttcaacgaa ctggattacg atctcgatcg cgacggacgt ctcgacggtc ctggtcgcaa
     5221 cggtattagt tggacaacgg acggcggact gacatggcag acctatgtgc cggaaaccga
     5281 tccggctttc gggaaaacgt ttcgtgcgtg gaacttcgcg tttgacggga ccactgtgtg
     5341 ggtagcggga tccgtatccg gtggcgatgc gctacttaca agtactgatc tcggtgcgtc
     5401 ttgggaactc caaccaatcg ttaccgtgga tggcgatgcg gtgcggtcgg cgagcggaac
     5461 cggggatgtc gcgtatttcg atggagtact atgggccaca accggtcaag ggcttgctcg
     5521 ttccgatgat cgcggacaga catggcaatt cgttctggcg tttcctcagg cggatccgct
     5581 cgatggcggt tctgtcgaag atcccgacgc ggcgcgatca aatctcgaaa cgtacgcgtt
     5641 tccgtcaccg tgttccccga tcagcgggac tccgccgaac atcgtcttcg cgttgacgca
     5701 accatcggat gtcacgatcg acatctatga cgcgggtagt ggtctggttc gcaccattca
     5761 tcgctcgagc gtctcggcag gtaaccacac tgtccgatgg gacggacgga ccgaaagcgg
     5821 acgatacgtc gcgaacggtg tttacctcta ccgtattacc
//
LOCUS       DSCC01000061            1528 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055434, whole genome shotgun sequence.
ACCESSION   DSCC01000061 DSCC01000000
VERSION     DSCC01000061.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055434"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(155..1483)
                     /locus_tag="ENN56_00690"
     CDS             complement(155..1483)
                     /locus_tag="ENN56_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74033.1"
                     /translation="MKNERGTRELIYQYSVGDLSALTDDEAKWTIRELAVRGIGVISN
                     WRSGEKQDASIAESLRIGRIQQSLGLTVAADANYILHRFYDNTSATAHVDDAGEPFFD
                     DTFSGPKMGCPFTLDARKPVILGRVAAFVEAYHLAGVTLGIVTSDWEIDGPHEWNGAW
                     EHAKRCARCLANLPNIEDFTAFQAKMRQMRAELLRDCYTMPILHRNPNALVTNYAVYP
                     NDGWRYWFDYFEKQQPELPHRMDQQHIDRPWYNDFGETGFTLAMPVVYTWHDIYPAYP
                     EFSDPDYRWMYNMLLVGTNAAKSTPSGIPIATFVHWHTTALPAGVDAPPQMSKTAYRE
                     LLWHLLLRGHDIFYSWTPMDELAEEIALVQEVYDASLAYSEWLRHGTPVTFDVPKTQG
                     AVVSGMRVNDRVLVRRTDFGDSAGRDVTIDVDGARISIPSKPGELQVITL"
BASE COUNT          293 a          504 c          447 g          284 t
ORIGIN      
        1 ccgccgatcc agctacatta cggctttcac agcctcgggg cgcacgatct ggcggcactg
       61 gcgaggaggc tgcgcgcgca gcctcctcat tcgtcaacgc catgggaaga agatacaagg
      121 gcgaggcatg cctcgccctt accgctacgt ccgcctacag cgttatcacc tgcagctcgc
      181 ccggcttcga cggaatgctg atccgcgcgc cgtcgacatc gatcgttacg tcgcggccgg
      241 cggagtcgcc gaaatccgtg cggcgaacca acacgcggtc attcacccgc atcccggata
      301 ccacggcgcc ctgcgttttg ggcacgtcga aggtgaccgg cgtgccgtgc cgtagccact
      361 ccgaatacgc cagcgacgcg tcatacacct cctgcacgag cgcgatctcc tccgccaact
      421 catccatcgg cgtccacgag tagaagatat cgtgaccgcg gagcagcaag tgccagagta
      481 gctcgcgata cgccgtcttc gacatctgcg gcggcgcatc cactcctgcg gggagagccg
      541 tcgtgtgcca atgcacgaac gtcgcgatcg gaataccgga tggcgtcgac ttcgcggcgt
      601 tcgtgccgac gaggagcatg ttatacatcc accggtaatc ggggtccgaa aactccggat
      661 acgccgggta gatgtcgtgc cacgtgtaga ccaccggcat cgcgagcgtg aatccggtct
      721 cgccgaagtc gttgtaccac ggtcggtcga tgtgctgctg gtccattcga tgcggtaatt
      781 cgggctgctg cttctcgaaa tagtcgaacc aataccgcca cccgtcgttc ggatacaccg
      841 cgtagttcgt cacgagcgcg ttcggattcc ggtggagtat cggcatggtg tagcagtcgc
      901 gtagcaactc ggcgcgcatc tgccgcatct tcgcctgaaa cgcggtgaag tcctcgatat
      961 tcggcaagtt cgcaaggcat cgcgcacacc gcttcgcatg ctcccacgcg ccattccact
     1021 cgtgcggacc gtcgatttcc cagtccgacg taacgatacc cagcgtcacg cccgccagat
     1081 gatacgcctc cacaaacgcc gcaacacgac cgagaatcac cggcttacgc gcatccaacg
     1141 tgaacgggca gcccattttc gggccgctga acgtgtcatc gaaaaacggc tcgcccgcat
     1201 cgtccacgtg cgcggtcgcc gacgtgttgt cgtaaaacct gtgcaggata tagtttgcat
     1261 ccgccgcgac cgtgaggccg agcgactgct ggatacggcc aatccgcaac gattccgcga
     1321 tcgatgcgtc ctgcttctcg ccggaccgcc agttgctgat gacgccgata ccgcgcaccg
     1381 caagctcgcg aatcgtccac ttcgcctcgt cgtcggtcag cgcgctcaga tcaccaacgg
     1441 aatactggta gatcagctcg cgcgtgccac gttcgttctt caagggaggt agggcgtcga
     1501 gtacggtgcg gatgggttcg ggaagggc
//
LOCUS       DSCC01000062            1854 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042741, whole genome shotgun sequence.
ACCESSION   DSCC01000062 DSCC01000000
VERSION     DSCC01000062.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042741"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            417..1400
                     /locus_tag="ENN56_00695"
     CDS             417..1400
                     /locus_tag="ENN56_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDS74034.1"
                     /translation="MSQEDGMDHYNDISRNNEEVHRHDHEHHHAENDRSGESTARLGI
                     VFALVVVFMFVEAVAGWLTNSLALLADAGHMLTDAGAIALALFASWLSRRPSGPQRTY
                     GYYRAEILAALANAVTLIVIVVFIVIEAYHRLAEPPTVMGLPMMAVASVGLVVNLIGM
                     WILGGNHGHDHGRQGLNLQGVLWHIIGDALGSIAAIVGGLLVWWQGWYWADPLVSVLI
                     SLIILYGGVRLVLKSVSVLMEVSPARIDVREVQHALKQLPGVVECHDLHIWTVTSGLE
                     SLSVHLVVEPFPADDDFLTRARELLHERFALNHVTIQVESIGFSHVECDFV"
     gene            complement(1418..1837)
                     /locus_tag="ENN56_00700"
     CDS             complement(1418..1837)
                     /locus_tag="ENN56_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74035.1"
                     /translation="MSTEIDVPIRQTVAFSVPHEPGRQWFSIYGTKAHVEWKRAHWDE
                     PKMYVDGQEVRDMNRMDWPVAPDWLTYLAGMEGHGGIDAALVEDFVDALRNGKPSPID
                     VHTGLDYSLPGFYAAMSARAGGELLRIPNSRTERIVP"
BASE COUNT          345 a          540 c          583 g          386 t
ORIGIN      
        1 acagccgaac gaaaaacgat acaaacgcag aggccgtcgg gagtgatctc ggcggcctcg
       61 ttgtgtttcg gcgaaggcga acgcaacggg gggggcggtg cgcggagata tgcccggcga
      121 atgaattcgc gggctgtgat cacgaagtct gccgcgaact ctcacatgct ttcgagttcg
      181 tcaaggcgaa gcttcgcttc gcccaatgtc tccgagcgca gcgaggagac gcgcgacttt
      241 agtcgcaccg cgaaaccgac attacagtac cccgccaacc gtaccgcgaa atgaacgagc
      301 cgtgctcccc gtaccccttt cgcgcgaagc tgcgcccgtt gacttccccc atctaccaac
      361 gtagcgttac ggcgttttcc gtgcatcgaa acactccttg agattcggag ccgaccatgt
      421 cccaagaaga cggcatggat cattataacg atatttcgcg aaataacgaa gaagtccacc
      481 ggcacgatca cgagcaccat cacgccgaga atgatcgatc gggtgaatcg acggcgcgac
      541 tcggcatcgt gttcgcgctt gttgtcgtgt tcatgttcgt cgaagcggtc gccggatggc
      601 tcacgaacag tctcgcgcta cttgccgatg ccgggcacat gctcaccgac gcgggtgcga
      661 tcgcgcttgc actgttcgcg tcgtggcttt cgcggcggcc gagtggtcca cagcggacgt
      721 atggctacta ccgcgcggag attctcgcgg cgctcgcgaa cgcggtcacg ttgatcgtga
      781 tcgtcgtgtt catcgtgatc gaagcgtacc atcgactcgc tgaaccgccg acggtcatgg
      841 ggctacccat gatggctgtt gcgagtgtcg gactcgtggt gaacctcatc gggatgtgga
      901 tcctcggcgg gaatcacggg cacgatcacg gacggcaggg gctgaatctt cagggcgtgc
      961 tctggcacat catcggtgac gcgctcgggt ccatcgcggc gattgttggc ggattgctcg
     1021 tgtggtggca gggctggtac tgggctgatc cgctcgtgag cgtgttgatc tcgctgatta
     1081 ttctctacgg cggcgtgcga cttgtgctca aatcggtcag cgtgctgatg gaggtgtcac
     1141 cggcgcgaat tgacgtgcgc gaggtgcagc acgcgcttaa gcagttgccg ggcgtcgtcg
     1201 aatgtcacga cctacacatc tggaccgtaa ccagcgggct ggagtcgcta agcgtgcatc
     1261 ttgtcgtcga gccgtttccc gccgatgatg atttcctcac gcgcgcccgc gaactccttc
     1321 acgagcggtt tgcattgaat catgtgacga ttcaggttga gtcgattgga ttttcgcacg
     1381 tcgagtgcga cttcgtgtga gcatcattcc cgaagcgtca gggaacgatg cgctcggtgc
     1441 gagagttggg aatacggagc agctcaccac cggcgcgcgc ggacatcgcc gcgtagaagc
     1501 ccggaagtga atagtcgaga ccggtgtgca cgtcgatcgg ggacggcttc ccgtttcgca
     1561 gcgcgtcgac gaaatcctcg acgagcgccg catcgatgcc gccgtgacct tccatgcccg
     1621 caagatacgt cagccagtcc ggggcgaccg gccagtccat gcggttcata tcgcgcactt
     1681 cctgtccgtc gacgtacatc ttcggttcat cccagtgtgc gcgcttccac tcgacgtgcg
     1741 ccttcgtgcc gtagatactg aaccactgac ggccgggttc gtgcgggacg gagaaggcga
     1801 cggtctgtcg tatcgggacg tcgatttccg ttgacatgat cgcgacttgg aggt
//
LOCUS       DSCC01000063            1696 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048153, whole genome shotgun sequence.
ACCESSION   DSCC01000063 DSCC01000000
VERSION     DSCC01000063.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048153"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..720)
                     /locus_tag="ENN56_00705"
     CDS             complement(<1..720)
                     /locus_tag="ENN56_00705"
                     /inference="COORDINATES: protein motif:HMM:PF13435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74036.1"
                     /translation="MAKRLRELAYALLTICLAVGIAHATHGVFPIQVMGISPHDIEAN
                     ALDVDYPDIKPTAGIPTVPVGHRVFVEAIEDTLIDISWEITGAPVGSGVAGVTGADTV
                     FTFVADLEGSYEVSLSLNSGATTATLWVTAAEFVGVGNIGGQTPSAMEGECAGCHAGK
                     AADWQTHVHATATVPNLTLPYMNESCMSCHATGYDSQKWADDLGFAFPQPGVGVYDSL
                     TTNHPDQSVFFNVQCESCHGPG"
     gene            complement(756..1415)
                     /locus_tag="ENN56_00710"
     CDS             complement(756..1415)
                     /locus_tag="ENN56_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74037.1"
                     /translation="MVSIVQKRKWVPVVPAIALIGLVILGVRLSTLDGVHATNRGFSR
                     VDSLECSRPQSMPDGIAVYEMQETHIPSVRNIIDPTAPNYAVPTNSRYGVLGAFPGDT
                     TFVVEQGQYLVATRLIGQTGDTLRVQWGNQTFPIIAVYGGGVGWKQRYITVHGGKHYM
                     LPIQWNEQASTWEPYNLDRWFAGTGPSVPRAEDSLEGRCSDCHTVADMSRSGMVFAGS
                     E"
BASE COUNT          366 a          512 c          467 g          351 t
ORIGIN      
        1 gccgggacca tggcacgatt cacactgcac gttaaagaaa accgactggt cgggatgatt
       61 cgtggtcagg ctgtcgtata cgccgacacc cggctgcggg aaggcaaagc cgagatcatc
      121 ggcccacttc tgcgaatcgt aaccggtcgc gtgacacgac atgcaactct cgttcatgta
      181 cggcagcgtg agattcggca ccgtggccgt cgcatggacg tgcgtctgcc agtcggcggc
      241 cttgccggcg tggcagccgg cgcattcgcc ttccatcgct gacggcgtct gcccgccgat
      301 gttaccgact ccgacaaatt cggcagcggt gacccacagc gtcgcggtcg tcgcaccgga
      361 atttaggctc aagctcactt cgtacgagcc ttcgaggtcg gcaacgaagg taaacaccgt
      421 gtccgcgccg gttactccag caacgccgga gccgaccggt gcgccggtga tttcccacga
      481 gatatcgatg agcgtgtcct cgatcgcttc aacgaacacg cggtggccga cgggaacagt
      541 aggaatacct gccgtcggtt tgatatcggg gtagtcgaca tcgagtgcat tcgcctcgat
      601 atcgtggggc gatatgccca tgacctggat cgggaagacg ccatgcgtcg catgtgcgat
      661 cccaacggcc agacagatgg tcaaaagcgc gtacgctaat tctctcagtc gttttgccat
      721 cgatgcacct ccttgagata cgagaaaacg ttgattcact ccgaacctgc gaacaccatc
      781 cccgatcggc tcatatcggc gacggtatga caatccgaac aacgcccttc gagtgagtct
      841 tccgcacgcg gaaccgacgg tccggtacca gcgaaccagc gatccaagtt atacggctcc
      901 cacgtcgacg cctgttcatt ccactgtatc ggcaacatat agtgttttcc gccatgaacg
      961 gtgatatagc gttgtttcca ccccacaccg cctccgtaca ccgcaatgat cggaaacgtc
     1021 tgattacccc attgcacgcg caaggtgtcg cccgtttgcc cgatgaggcg cgtcgcgaca
     1081 agatattgcc cctgttcgac gacaaaggtc gtatcgccgg ggaacgcacc gagcacacca
     1141 tatcgagaat tcgtcggtac cgcgtaattc ggcgcggtag gatcgatgat gttgcgcacc
     1201 gacgggatgt gcgtctcctg catctcgtac acggcgattc cgtccggcat cgattgcggt
     1261 cggctgcatt cgagcgaatc gactcgtgag aacccgcggt tcgtagcgtg cacgccatcc
     1321 agcgtactca gcctcacgcc gagtatcacc aggccgatga gcgcaatcgc cggcactact
     1381 ggcacccatt tgcgcttttg aacaatcgat accaccgcgg acaccgtctc tttgtcgttc
     1441 gcttggaatt accactcagt cgcattcctg actgaggaga cgtttgttat tgcaaaggcc
     1501 gtaccgcgaa ccacggattg cgattcgaag gagagatacc gatgtatgcg ggacttccgg
     1561 aagatcgcgc gagaaaagat gtttgctcga catgtgtcac cagcgacaca ctcatttgcc
     1621 gagaaggatt gtatgtagct cgtagtatta tacttacgtc catacccgcg caagactcat
     1681 gtgtcaccgg tgacac
//
LOCUS       DSCC01000064            3548 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017804, whole genome shotgun sequence.
ACCESSION   DSCC01000064 DSCC01000000
VERSION     DSCC01000064.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017804"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(129..1214)
                     /gene="lpxK"
                     /locus_tag="ENN56_00715"
     CDS             complement(129..1214)
                     /gene="lpxK"
                     /locus_tag="ENN56_00715"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:PF02606.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDS74038.1"
                     /translation="MTRLDQWLSEKPETNWEESLLSGLHIASKFYTAGVRARAWAYRH
                     GVFRRISVKVPLVSVGNLTVGGTGKTPLTIALARMLLEHGRRPAIVSRGYFKEGRGLV
                     VVSDGNRLLVGRRQAGDEAYMMARALSGVPVVVGPNRSLAATVVQSRFAPDIIIMDDG
                     FQHLKVQRDLDIVVVDATNPFGNGMTLPRGTLREPPNHLRRADMIVLTHTDQTEQIDC
                     LRDSLHALNPNAPIVESVHQPVSLSNYQQQSELELGTLRGARVVALSSIGNPRTFERT
                     LTDLGAELVDFCRLPNHYAYRRGGLSDLVDRARDLRVKAIVTTEKDMVRFPPGFAFNI
                     PVWILGIKLHITAGNEYLERVWELGQA"
     gene            complement(1258..2580)
                     /locus_tag="ENN56_00720"
     CDS             complement(1258..2580)
                     /locus_tag="ENN56_00720"
                     /inference="COORDINATES: protein motif:HMM:PF04413.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74039.1"
                     /translation="MLLRSKEEEIEYLLYENRDTDFETQGKSREILPPMNHYLAAYRV
                     AWLAGLAVTVPVWMFGALTDRWQVRRRFRPRVSDDGAPAWWWHGASAGEVRGLTFLLD
                     SVERTDNTVVDRVSATTESGLAMWHATGRDAAVLPVDLRYTIRRVLPRNLRLAVLSET
                     ELWPMLLDELVTRRVPIVVASARLSERALSRLRATRLLRANAGALYVCAQTDEDGQRY
                     RDLGVPHEHIAVTGSLKWPRRAPAGREAVRRRFRLTPNMPCVVAGSIRRDEIEVVASA
                     LARVRSAQSDVRVIIAPRMPSDVSYAQQRMWEAGFEPTPYDAFPEENRGSSTEPLRCT
                     VVGTMGELATLYAAADVAVVGGGWEPVGGHNPFEAAVYRIPIVYGPYMRQPGVERLEA
                     IGQATRATDERALASAILNGLSAGGFDAVSWPDPVEATLTAWRVWGIV"
     gene            2582..>3548
                     /locus_tag="ENN56_00725"
     CDS             2582..>3548
                     /locus_tag="ENN56_00725"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="can hydrolyze p-nitrophenyl sulfate; contains a
                     specific cysteine that is converted into
                     C{alpha]-formylglycine upon activation with the anaerobic
                     sulfatase-maturase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HDS74040.1"
                     /translation="MADTRPTNLLLITADQYRGDCLGLAGHPAVMTPNLDDWFNEGAY
                     FPRAYSECPSCIAARRTLITGMTPYGHGLPGYRDQVPFSPPTTMMRELHDNGWQTMCA
                     GKMHFTPQRKHHGYETMILYEGQQRFGDYVDDYEEWLKARTPLTERVHGVDSNSWMAR
                     PSHLPEELHNTTWVVDQAIELLRRRDPTRPFFLWVSFHRPHAPLDPPQVYWDMYTDAE
                     LPAPVISEWSLNHAVPPPLTPNAWEGYLTDRDQRRAQIGYYALITQIDFQIGRLFEEM
                     RRLKCGDNTFSVMTADHGEMLGDHHLWRKSYGYEGSARVPFVVRTG"
BASE COUNT          726 a         1085 c         1053 g          684 t
ORIGIN      
        1 ctcccgtcta caaaccaaag agaagcttag caggacatac aatataatcg cccggagaaa
       61 tcaaccaagg gatgggccgt ttgcgggtat cccgcactgg actcgatcat cgcacaggtt
      121 cggtcgcttt acgcttgtcc aagttcccac acccgttcga gatactcatt tcctgcagtt
      181 atatgcaatt tgataccgag tatccagacc ggaatgttga acgcgaatcc tggcgggaag
      241 cgcaccatat ctttctccgt cgtcacaatt gcttttacgc ggaggtcgcg cgcgcggtcg
      301 acgaggtcac taaggccgcc gcgacggtat gcgtagtggt tcggaaggcg acaaaagtcc
      361 accagttcgg cgccgagatc agtgagcgtg cgctcaaacg tgcggggatt cccaatgctc
      421 gatagcgcga caacgcgcgc gccacgcagc gtaccgagtt cgagttctga ttgttgttgg
      481 taattggata gacttaccgg ttggtggacg ctctcgacga tgggcgcgtt gggattcaac
      541 gcgtggaggc tgtcacgtaa acaatcgatt tgctcggtct gatcggtatg agtaagcacg
      601 atcatatcgg cgcggcggag gtgatttggg ggttcgcgta aggtgccacg aggaagcgtc
      661 atcccgttgc cgaacgggtt ggtcgcatcg acgacgacga tatcgaggtc acgttggact
      721 ttcaaatgct ggaagccgtc gtccatgatg atgatatcgg gtgcgaatcg actctgaacg
      781 acggttgcgg ccagactccg gttcggaccg acgacgacgg gcaccccgga gagggcacgc
      841 gccatcatgt aggcttcatc accggcttga cgacgcccaa ccagaagccg atttccatcg
      901 gatacgacga cgagtccgcg tccttctttg aagtacccgc ggctgacaat cgcaggacgc
      961 cgcccatgtt cgagcagcat ccgagcaagc gcgatcgtaa ggggcgtctt gccggtcccc
     1021 ccaacggtga ggttgccgac ggagaccagc ggtaccttca cggatattcg gcggaacacg
     1081 ccgtggcggt acgcccacgc gcgcgctcga acaccggccg tatagaactt cgaggcgatg
     1141 tgtaatccgc tgaggagcga ctcttcccag ttcgtttccg gtttttcact gagccactga
     1201 tccaagcggg tcatttcaga gttctctctt gatacctatc gtatcgttca tgcggtttta
     1261 cacaatgccc caaacgcgcc acgcggtgag cgtcgcttcc acgggatccg gccacgaaac
     1321 cgcatcgaat ccgcctgcgg acagaccgtt cagaatcgct gatgcgagcg ctcgctcatc
     1381 ggttgcgcgc gtagcctgtc cgatcgcttc gaggcgctca actccgggtt gccgcatgta
     1441 cgggccatac acaatcggaa tccgatacac ggccgcttca aacggattat gcccgccgac
     1501 aggttcccaa ccaccaccga cgaccgcgac atccgcggct gcgtacagcg tggcgagctc
     1561 ccccattgta ccaacgacgg tacatcggag tggttccgtg gaagagcctc ggttctcctc
     1621 gggaaacgca tcgtacggag tcggctcgaa accggcttcc cacatgcgtt gttgcgcgta
     1681 ggatacatca ctcggcatac gcggcgcaat gatgaccctc acgtcagatt gcgcggaacg
     1741 aacacgcgca agtgcggagg cgacgacttc gatctcgtcg cgccgaatcg atcccgcgac
     1801 gacgcacggc atgttcgggg tcagccggaa gcgccgccgc accgcttcac ggccggccgg
     1861 tgcacggcgc ggccatttga gggaaccggt caccgcaatg tgctcgtgcg gcactccgag
     1921 atcgcggtac cgctgtccat cttcgtccgt ttgcgcgcag acgtaaagcg cgccggcgtt
     1981 cgcgcggagg agtcgtgtcg cgcgaagtcg tgagagtgcc cgctcggaaa gccgcgcgct
     2041 cgcgacgacg attggaacgc ggcgcgtaac gagctcgtcg agaagcatcg gccacaactc
     2101 cgtctcgctc agaaccgcga gccggagatt ccgcgggagt actcggcgta tggtatagcg
     2161 gagatcgacc ggaagcaccg cggcgtcgcg accggtcgcg tgccacatcg cgaggccgct
     2221 ttcggtcgtc gcggatacgc ggtcgacgac ggtgttgtcg gttctttcca ccgaatcaag
     2281 caagaacgta agcccgcgca cctcgcccgc cgaagcgcca tgccaccacc acgccggagc
     2341 gccatcgtca gatacgcgcg gacggaagcg gcggcgcacc tgccagcgat cggttagcgc
     2401 accgaacatc catacgggaa ccgttaccgc gagaccggca agccacgcga cacgatatgc
     2461 agcgagatag tgattcatcg gtggaagtat ctcacgcgat ttaccttgcg tctcgaaatc
     2521 cgtgtcccga ttttcgtaga gaaggtattc gatttcctcc tctttcgatc ggagcagcac
     2581 gatggcggat acacgtccca caaacctgct tctgattacc gctgaccagt atcgcggcga
     2641 ttgtctcgga ctcgcagggc atcctgcagt tatgacgcca aatctcgatg attggttcaa
     2701 tgagggcgcc tacttccccc gcgcatacag cgagtgcccg tcgtgtatcg cggcgcgacg
     2761 tacgctgatc accggtatga cgccgtatgg gcacggtctg cccgggtatc gcgatcaggt
     2821 gccgttcagt ccgccgacta ccatgatgcg cgaactccat gacaacggtt ggcagacgat
     2881 gtgtgcggga aagatgcatt ttaccccgca gcggaagcat catggatacg aaacgatgat
     2941 tctctacgaa gggcaacagc gcttcggtga ctacgtcgat gattacgagg aatggttgaa
     3001 agcgcggacg ccgctgaccg aacgcgtcca cggagtcgat tccaactcgt ggatggctcg
     3061 gccctcgcac cttcccgagg agcttcacaa cacaacgtgg gttgtcgatc aggcgatcga
     3121 gctgctccgc cgtcgtgatc cgacccggcc attcttcctc tgggtgagct ttcaccgacc
     3181 gcacgccccg ctcgatccgc ctcaggtcta ctgggatatg tacaccgacg cggaactgcc
     3241 cgcgcccgtc atcagcgagt ggtcgctgaa ccacgcggtg ccgccaccac tgactccgaa
     3301 cgcgtgggag ggctacctca ccgaccgcga ccaacggcgc gcgcaaatcg gatattacgc
     3361 gctcatcaca cagatcgatt tccagatcgg ccgcctcttc gaagagatgc gccgactcaa
     3421 gtgtggcgat aacacgtttt ccgtcatgac ggccgatcat ggcgaaatgc ttggtgatca
     3481 ccatctctgg cgcaagtcat acgggtacga aggttctgcg cgggtgccgt ttgtcgtcag
     3541 gacgggac
//
LOCUS       DSCC01000065            4057 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1014850, whole genome shotgun sequence.
ACCESSION   DSCC01000065 DSCC01000000
VERSION     DSCC01000065.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1014850"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..640
                     /gene="ruvB"
                     /locus_tag="ENN56_00730"
     CDS             <1..640
                     /gene="ruvB"
                     /locus_tag="ENN56_00730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDS74041.1"
                     /translation="DYVIDIIIGQGPGTSSVRVELEHFTLIGATTRTGLLTSPMRDRF
                     SIVERLNYYTVDNLTSIVRRSSTILGTSVDEDGANELARRSRGTPRVANRLLRRARDF
                     AQVRGDGTITRDVAQRTLRLMDVDELGLDEMDKRILTTIMDKFGGGPVGINTIAAAVN
                     EQPDTVEEVYEPFLMQEGLLQRTPRGRLVTPIAYKHFGRKLSAQQRTLFESE"
     gene            673..1281
                     /locus_tag="ENN56_00735"
     CDS             673..1281
                     /locus_tag="ENN56_00735"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDS74042.1"
                     /translation="MAITTILFDLGKTLVDFDFEPVNQRLTESSKLSAEVAKQRTKDR
                     YNAFCRGEMTAVEWQKFIETDYNLRMSSEEFRRFWADIFWPIDKMFELAHRTKKHYRQ
                     YLLSNTDEIHLPWCMERYAERFEGLLDGMILSYEAGAIKPDRRIFEYGTERFDLVPEA
                     CVFIDDLPENIEAARDFGMHGIVITSPAQVESELQALGVRTA"
     gene            complement(1268..2260)
                     /locus_tag="ENN56_00740"
     CDS             complement(1268..2260)
                     /locus_tag="ENN56_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74043.1"
                     /translation="MIPFPSKPHQVDAKIVEWRDIAGDSLRADVLETFSGHQIHALTL
                     TNPHVPRDCKRALYVSQPHAHEPGATAGMVDVIEQLVRAFHLGGSPTMLDRDAILDRC
                     VLTFNPIGNPQGRSRAPVDVWTGNDYTNAEFWCWMRGEHPDKPEEMWERYDRYDDRSL
                     RTPERPGIVYEQIDEHVWVEPNRDHGSSYFRLFARMDGVYRYDRWLDLHQQEFVNSSY
                     RSEIVLPIQATLDGQPQAIRDEINGWGQEIVAAWSAAGFDPMPQPRALPYSGQQAEYF
                     VRTWGTLHQRMPIIFTEIKNNAPDAPPDFQIHSQAIAIETTIARMMRDLSTTQS"
     gene            complement(2329..3225)
                     /locus_tag="ENN56_00745"
     CDS             complement(2329..3225)
                     /locus_tag="ENN56_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HDS74044.1"
                     /translation="MVGTSAIQRGQPVTTHAQLEANSLGADDSLRHYLDDIRSIPMLN
                     RDEEVELATRARAGDVNARARLVQANLRFVISIAKRYQHRGISLVDLVAEGNVGLVKA
                     VDRFDETLGYKFISYAVWWIKQAIIQAISRQARPMRLPSARANLLYRIEKASGRLEQK
                     HGSSVDWRDIAEEVDSCEDDVREMLLFQPRVSSLDGSTSNEDVALLDVVFDEFANDPH
                     RELVENSRCAEINAALEALTDRERIIIERFFGLVGDRQTLEQIGVDFGLTRERIRQIK
                     DRALRKLRHFSRSGALREYLAA"
     gene            complement(3274..>4057)
                     /locus_tag="ENN56_00750"
     CDS             complement(3274..>4057)
                     /locus_tag="ENN56_00750"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255418.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDS74045.1"
                     /translation="ENTEDVYAGLELEAGTEGAAKLIDFCAAEFGWNIRPDSGIGIKP
                     ISLAGSERLIRAAIEYAIQNDRKSVTLVHKGNIQKFTEGAFRKWGYELAKREYAEKVV
                     SWEECGGNPPEGKILIKDAIADIFLQQILTRPAEFEVVATMNLNGDYFSDALAAQVGG
                     IGIAPGGNINYVTGHAIFEATHGTAPKYADQDKVNPGSVILSGEMMLRYLGWTEAADL
                     IVHGLEKTIAAKTVTYDFERLMDGAKLLKTSEFGSAIIKNMD"
BASE COUNT          857 a         1172 c         1094 g          934 t
ORIGIN      
        1 agattacgtc atcgatatta tcatcggaca gggacccgga acatcgtcgg tccgcgtcga
       61 gctcgaacac ttcaccctca tcggtgcgac gacgagaacc ggcctactta cgtctccgat
      121 gcgcgatcga tttagcatcg tagagcggct gaactactac accgttgata acctcacttc
      181 gattgtccgt cggtcgtcta cgatactcgg cacgtcggtc gacgaagacg gcgcgaacga
      241 actcgcgcgg cgctcgcgcg gaacgccgcg tgtcgcgaat cggcttctcc gccgtgcgcg
      301 tgacttcgcg caagtccggg gagacggaac aatcacccga gacgtcgcac aacgaacgct
      361 ccgtttgatg gatgtcgacg aacttggtct tgacgaaatg gataagcgca tcctcaccac
      421 gatcatggat aaattcggtg gcggtccggt gggtatcaac accatcgccg cggcggtgaa
      481 tgagcaacct gatacggtcg aagaggtcta cgagccattc ctcatgcagg aaggccttct
      541 tcagcgaaca ccacgcggtc ggttggttac gccaatcgcg tacaaacact tcggccgaaa
      601 gcttagcgct cagcagcgca cgctgttcga atcggaatga cacgttgatc ggagttggaa
      661 ccggtaggat atatggcgat taccacgatt ctttttgatc tcggaaaaac gctcgtcgat
      721 tttgacttcg agccggtaaa ccagcgactc accgaatcat cgaagctttc tgcggaagtc
      781 gcgaaacaac gaacgaagga ccggtacaac gcgttctgtc gcggcgagat gaccgcggtg
      841 gagtggcaga agtttatcga gacagattac aacctgcgca tgtcgtcgga ggagtttcgg
      901 cgcttttggg ccgatatctt ttggccaatc gacaaaatgt tcgagcttgc tcatcggacg
      961 aaaaaacact accgccagta tctcctctcg aataccgatg agatccactt gccgtggtgt
     1021 atggaacgct acgccgaacg cttcgaaggg ttactcgacg ggatgatcct gtcatacgaa
     1081 gcgggtgcta tcaagcccga tcgacgcatc ttcgagtatg gtaccgaacg gttcgatctc
     1141 gtaccggaag cctgtgtttt tatcgacgat ttgccggaga atatcgaggc cgcgcgcgat
     1201 ttcggcatgc atgggatcgt gattacgtcg cccgcacagg tcgagagcga actgcaggca
     1261 ctcggtgtca ggactgcgta gttgatagat ctcgcatcat acgcgcgatc gttgtttcga
     1321 tcgcgatagc ctgagaatgt atctggaaat ccggcggcgc gtcgggtgcg ttgtttttga
     1381 tctcagtgaa gatgattggc attcgttggt gcaacgtgcc ccacgtccgc acgaaatact
     1441 cggcttgctg ccccgaatac ggtaacgctc gcggctgtgg catcgggtcg aatcccgctg
     1501 cactccacgc ggcgacgatt tcttgacccc acccattgat ttcatcgcga atcgcttgtg
     1561 gttgcccatc caaggttgct tgaatcggga gaacgatctc cgaccgatac gaggagttca
     1621 cgaactcctg ttgatggaga tcaagccaac ggtcgtaccg gtagacgccg tccatcctcg
     1681 cgaaaaggcg aaaatacgac gacccatgat cacgattcgg ctcgacccat acgtgttcgt
     1741 cgatttgctc gtacacaatc cccggtcgtt cgggcgttcg taaagaccgg tcgtcatacc
     1801 ggtcatatcg ctcccacatc tcttccggtt tatcgggatg ctcgccacgc atccaacacc
     1861 agaactccgc attcgtgtaa tcgttgcccg tccacacgtc gaccggtgcg cgactgcgtc
     1921 cctgcggatt cccaatcgga ttgaacgtga gaacgcaccg gtcgagtatc gcatcgcggt
     1981 ctaacatcgt cggtgaaccg ccaaggtgaa acgcacgaac cagctgctcg atgacatcga
     2041 ccattcccgc agtcgcacct ggttcgtgag cgtgtggttg cgagacgtag agcgctcgtt
     2101 tgcagtcccg cggaacatgt ggattggtca atgtcagcgc gtggatttga tgaccgctga
     2161 acgtttcgag gacgtctgcg cgcagcgaat cgccggctat gtcgcgccat tcaacgattt
     2221 ttgcgtcaac ctgatgcggt ttacttggga atgggatcat ggagtacaag gtaacggcgt
     2281 caattccacc ggggaaccga cgccgtatcg tgatgttggt gtatggtctt acgcggcgag
     2341 atactcacgt agtgcgccgc tacgagagaa gtgacgcagc tttctcagtg cgcgatcctt
     2401 gatctgccga atacgttcgc gcgtaagccc gaagtctacg ccaatttgtt ccagcgtctg
     2461 acggtcgccg acaagtccga agaaccgttc gatgataatc cgctctcgat cggtcaaagc
     2521 ctccaacgcc gcgttgattt ccgcacacct gctgttttca accagctcac gatgcggatc
     2581 gttcgcaaac tcgtcgaata ccacgtcgag aagcgccaca tcttcgttgc tcgtgcttcc
     2641 gtcgagcgaa ctcacgcgcg gctggaaaag gagcatctcc cgtacgtcgt cctcgcacga
     2701 gtcgacctct tccgcaatat cgcgccaatc aaccgaacta ccgtgcttct gttcgagtcg
     2761 accgctggcc ttctcaatcc gatagaggag attcgctcgc gccgacggta gccgcatcgg
     2821 acgtgcctgt cgggaaatcg cttgaatgat cgcttgcttg atccaccaca cggcgtacga
     2881 gataaacttg taaccaagcg tctcgtcaaa gcggtcgaca gctttgacga gaccgacgtt
     2941 accctctgca acgagatcga cgagtgaaat gcctcggtgc tgataccgtt tcgcgatgct
     3001 gatgacgaag cggagattcg cttgcacgag ccgtgcccga gcgttgacat cacccgctcg
     3061 cgcacgagtc gcgagttcga cctcctcatc gcgattcaac attggaatcg agcggatatc
     3121 gtcgagatag tggcgcaagc tgtcatccgc tccaaggctg ttcgcttcga gttgtgcgtg
     3181 cgtcgtcact ggttgccccc tctgtatcgc tgaggtgccg accaactccg aacgctacca
     3241 cactaactcg gtcggcgtac gccgtgggac acctcagtcc atgttcttga ttatagccga
     3301 gccgaactcc gacgttttca acaacttcgc gccgtccatg agtcgctcga aatcgtacgt
     3361 tacggtcttt gcggcaattg ttttctcgag tccgtggaca atgagatcgg ccgcctctgt
     3421 ccagcccaga tatctgagca tcatctcccc cgaaagtatg acgcttccgg ggtttacttt
     3481 atcctgatca gcatattttg gggctgttcc gtgcgttgcc tcgaaaatgg cgtggcccgt
     3541 cacgtaattg atatttccac ccggggcgat tccgatgcca ccgacctgtg ccgcaagtgc
     3601 gtcggagaag tagtcaccgt tcagattcat cgttgcgacg acttcgaact ccgcaggacg
     3661 cgtgagtatt tgctggagga atatgtcggc gatcgcatcc ttgatgagga ttttgccttc
     3721 gggcggattc ccgccacatt cttcccagct aacgaccttt tccgcatact cgcgtttcgc
     3781 gagctcgtaa ccccatttac ggaaagcgcc ttcggtgaat ttctgaatat tgcccttgtg
     3841 tacgagcgtt acgcttttac gatcgttctg gatcgcgtat tcgattgccg cgcgaatcag
     3901 ccgttcgcta ccggcaagac tgataggttt gattccgatc cccgaatccg gtcggatatt
     3961 ccatccaaat tccgccgcac aaaaatcgat cagcttcgct gcgccttccg tgccggcttc
     4021 caactcgagc cccgcgtaaa cgtcctcggt attctcg
//
LOCUS       DSCC01000066            3240 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020166, whole genome shotgun sequence.
ACCESSION   DSCC01000066 DSCC01000000
VERSION     DSCC01000066.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020166"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            428..1111
                     /locus_tag="ENN56_00755"
     CDS             428..1111
                     /locus_tag="ENN56_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDS74046.1"
                     /translation="MKRTLLTASIMVVLVAAFSMPGCGWSNKAKGALVGSGAGAAVGG
                     YIGSRSGNTAAGAIIGAAVGGAAGTAIGHYMDSQKEELDEELQNAKIERVGEGIKITF
                     DSGILFQTASADLQPASEENIAGLANILQKYADTNILVEGHTDSQGSEAYNQTLSEQR
                     AASVASKLLDLGVAPERVTTVGHGEMMPVADNETAEGRALNRRVEIAIFANDALKEKA
                     ESGELGSGS"
     gene            complement(1201..2931)
                     /locus_tag="ENN56_00760"
     CDS             complement(1201..2931)
                     /locus_tag="ENN56_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDS74047.1"
                     /translation="MASIPRAEYPRPNCVREQWRNLNGRWQFEMDPGLSGVARGLPDA
                     DSLVGEIVVPFCMESALSEVHHVDFMEGVWYRRTFSVPEAWAGMRIMLRFEAVDYHTR
                     AWVNGTFVGQHRGGYSPFGFDITDALRRGDNVLTVFAEDDTRSPMQPSGKQAERYASH
                     GCHYTRTTGIWQTVWLEPVPAAHIDRLHMTPDPESGVLRVDVHTSVAASGGSVRLVVS
                     ADGDTVADLTAPVVGTVARVNAQLRDVRWWSQADPFLYDVTVTMISPNGERDVVTSYA
                     GMRNLGIRGMALLLNGKPKFQRLVLDQGFYPDGIYTAPTDDALRRDIEMSQAFGFDGA
                     RLHMRVFERRFLYWADKLGYLVWGEYPNWGIAHSNPATLARVHEEWTEVMKRDINHPS
                     IVGWCPFNETARDQDADTLRAIFRTTKLADPTRPCIDTSGYTHTELTEIYDVHCYEQD
                     PAKFTERFQPMLTGGETWRNFPDHDAAYAGQPYFVSEYGGIWWNPGQRGTDAWGYGDR
                     PTSEAEFVERYRKLTTALLRHPMMCALCYTQLYDVEQEVNGLATYDRKVKFDPQIIRD
                     INQQVAAIEE"
BASE COUNT          669 a          992 c          944 g          635 t
ORIGIN      
        1 gaagcgactc tgtcgcttcg ccccacgtct ccgagtgaaa cgaggagatg cgggtggttt
       61 caaccgcacc gcgcgaaaaa acgtaacgcg aaaccgaagg tttgcggtag gagcccagcg
      121 gataaatccg cgggcattta ttgcgaagtc cgcctacgcg gactatgggg acaactaacg
      181 tgagcggttg agcctgcgta ggcaggcttt gtaattgtag cgggcgactt cagtcgcacc
      241 gcgaactcgt gcgtagcgag gagacgcacg gggaccgcgt tgaataccga tgatgtgacg
      301 ggtgtcacgg ttttcttacg agaaattcgt atattcacga cgggatacag ccttttttta
      361 ccgtatgcgc gaccgcagag ggccgtggca acggatgtaa cacaccccga tgtcgggagg
      421 attaacgatg aaacggactc tactcacagc gtcgatcatg gtcgtactgg tagcggcgtt
      481 ctcgatgccg ggttgcggct ggagcaacaa ggcgaagggc gcgctcgtcg gcagtggcgc
      541 aggtgcagca gtcggtggct acatcggcag tcgttccggt aacaccgcag cgggcgcaat
      601 catcggcgcg gccgttggtg gtgctgccgg caccgcgatc ggtcactaca tggacagcca
      661 gaaggaagag ctcgacgaag aactgcagaa cgcgaagatc gaacgtgtcg gtgaaggtat
      721 taagattacg ttcgattccg gtattctctt ccagaccgcg tccgccgatc tgcagcccgc
      781 atctgaggag aacatcgccg gtctcgcgaa tattctccag aagtacgccg acacgaacat
      841 tctcgtcgag ggacacacgg attcgcaggg ctccgaagcc tataatcaga ccctcagcga
      901 acagcgcgca gcttccgttg catcgaagtt gctcgatctc ggcgtagcgc ctgaacgcgt
      961 cacgaccgtt ggccacggcg agatgatgcc ggtcgccgat aacgaaacgg cggaaggccg
     1021 cgcactcaac cggcgtgtcg aaatcgctat cttcgcgaat gacgcgctca aagaaaaggc
     1081 ggaaagcggc gaactcggct ccggttcatg acgacgctga aacacctgct tcgctaaaca
     1141 acgacggcgc cgcaatttac acggcgccgt cgtcatttaa tacgatctat tccgcgcgct
     1201 ttactcttcg atcgcggcga cctgctggtt gatgtcgcga atgatctgcg gatcgaattt
     1261 caccttccga tcgtacgtcg cgagaccgtt gacctcctgc tcaacgtcgt acaactgcgt
     1321 gtagcacagc gcgcacatca tcggatgccg aagcagcgcc gtcgtcagct tgcgatatcg
     1381 ctcgacaaac tccgcctcgc ttgttggacg gtcgccgtat ccccacgcat cggttccacg
     1441 ttgccccggg ttccaccaga tgccgccgta ttcgctgaca aaatacggtt gacccgcata
     1501 cgccgcgtcg tggtcgggga aatttcgcca cgtctcgcca ccggtcaaca tcggctgaaa
     1561 ccgttcggtg aacttcgcag ggtcctgctc gtaacagtgc acgtcgtaga tttccgtgag
     1621 ctccgtgtgc gtgtagcctg aagtatcgat acacggacgc gtcggatcgg caagtttcgt
     1681 tgtgcggaaa atcgcgcgca gcgtgtccgc gtcctgatca cgtgctgttt cattgaacgg
     1741 acaccacccg acgatcgacg gatgattgat atcgcgcttc atgacttcgg tccactcctc
     1801 gtgaacccgc gcaagtgtcg cgggattcga gtgcgcgata ccccagttcg gatactcgcc
     1861 ccacacgagg tagccgagtt tatctgccca gtagagaaac cgccgttcga acacgcgcat
     1921 gtgcagtcgc gcgccgtcga atccgaacgc ttgcgacatt tcgatgtcgc ggcggagtgc
     1981 gtcatcggtc ggagcggtgt agatgccgtc cggatagaac ccctgatcga gcacgagtcg
     2041 ctggaacttc ggctttccgt tcaacaggag cgccattcca cggataccca gattgcgcat
     2101 tcccgcgtaa ctcgtcacca cgtcgcgctc gccgttggga ctgatcattg tcactgtgac
     2161 gtcgtaaagg aacggatccg cctgcgacca ccatcgcaca tcccgaagct gcgcgttcac
     2221 gcgagcgacg gtgccaacaa ccggcgcggt aagatcggcg accgtatcac catcggcact
     2281 gacaacgagc cgcacgcttc cgccgcttgc cgcgaccgac gtgtggacgt ccacccgcaa
     2341 cacgcccgat tccgggtccg gcgtcatgtg cagccggtcg atgtgcgccg cgggtacagg
     2401 ttcgagccac accgtctgcc agatgccggt cgtacgggtg tagtggcatc cgtgcgacgc
     2461 ataccgttcc gcctgtttcc cactcggttg cattggtgat cgcgtgtcat cctcggcgaa
     2521 caccgtcagg acgttgtcgc cacgtcgtag cgcgtcggtg atgtcgaatc cgaacggtga
     2581 atatccgccg cgatgttggc cgacaaatgt gccgtttacc catgcccgtg tatggtaatc
     2641 caccgcctcg aaccgcagca tgatccgcat gcctgcccat gcctccggaa cggaaaacgt
     2701 ccgccgatac catacgccct ccatgaaatc gacgtggtgc acctcggaga gtgcgctctc
     2761 catgcagaac ggcacgacga tctcaccgac gagcgagtcc gcgtcgggaa gaccgcgcgc
     2821 aacgccgctc agacccggat ccatttcaaa ctgccaccgt ccgttcagat tccgccactg
     2881 ttcgcgcaca cagttggggc gcggatactc ggcgcgcgga attgaagcca tcgggaatac
     2941 tcctcttcta cggttttcaa tgcacggaat cattaaggta gcgcgggaac gcgtgtgaag
     3001 ggatggtgtc agatgctctc agcggcgata tgcaagcttc cacgtcggca caaatgcgac
     3061 aggccggaac gcacgcttgt gctgcgcgag tccctcgtgg ccgagatcgc ccgcatcgtt
     3121 cacgtatcgg catccgtcga gccgtcgcag caattcaaca cgcgtcgttt cgctgagtcc
     3181 gacgatcgac ggatcgctct tctgcacgaa gaactgccca atgccggctc gcaacacgcc
//
LOCUS       DSCC01000067            3615 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017329, whole genome shotgun sequence.
ACCESSION   DSCC01000067 DSCC01000000
VERSION     DSCC01000067.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017329"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..180)
                     /locus_tag="ENN56_00765"
     CDS             complement(<1..180)
                     /locus_tag="ENN56_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74048.1"
                     /translation="MKAEWIELAHGWKFLRDEAPDAWQPKFDDANWETVSVPHCFNAA
                     DSFIPEQRFYRGPGWY"
     gene            387..929
                     /locus_tag="ENN56_00770"
     CDS             387..929
                     /locus_tag="ENN56_00770"
                     /inference="COORDINATES: protein motif:HMM:PF13801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HDS74049.1"
                     /translation="MKRSFVSLIAIAAAGLFAVTANAQPSPGMGAGSNSNPGICDSSG
                     PHGPQMGGGGGHMGQRGNAKVMLHRIFALDLTSEQATRVRAIQQNVEKQIIPKRATLD
                     VKRIELRELMHQTNVNQRAVTNKLKEINDVRGEIAQLRVNAQLEARGVLTGEQLERYL
                     DASWRPETSQCRTNGPARRR"
     gene            941..1600
                     /locus_tag="ENN56_00775"
     CDS             941..1600
                     /locus_tag="ENN56_00775"
                     /inference="COORDINATES: protein motif:HMM:PF13801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HDS74050.1"
                     /translation="MRTHHSARGDLCKVAPCSLSITHPNVGDAMNPFRRVIPFAFAVC
                     VAVVSIAVAGSRSPDATLEPETDDNPPAFFGAGQYAPQALLFRMNRLDLRPDQRDDIR
                     AILTVARESATPLSTVIREREAALTERLLADVIDRDGVRADLESIGVMRDEMNALWLD
                     AYFNAWERLDDDQRANLREIAKEPVQLDRRRSPDADRSGRPRGPRPGDGIGRPGGFGG
                     W"
     gene            1820..2422
                     /locus_tag="ENN56_00780"
     CDS             1820..2422
                     /locus_tag="ENN56_00780"
                     /inference="COORDINATES: protein motif:HMM:PF13801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HDS74051.1"
                     /translation="MGQALDRRPEEAASMMLDRIGQLPLTSEQREELRTLRARAERDV
                     APLQEQVREKEQQLYDLLVEGEVSEAEVQKQVDEIAGLRKQVAMRYVNCYRNMNGQLS
                     TDQRTRLRRMSREQDDAERGNMQGAGRSQSERPGQAGGAGSAASGRGSGAGADESGAP
                     TTQQQTDDEAEGAIETPEPVDEATGGAGGSQSGTRGRGRR"
     gene            2594..3091
                     /locus_tag="ENN56_00785"
     CDS             2594..3091
                     /locus_tag="ENN56_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDS74052.1"
                     /translation="MNDPITVLKTRRSVRSFRTTPIPQETLEDIIDCARLAPTGRGVQ
                     PWEFIVVTDADTRKGIAEIADYGSFIADAPACVAVFCADTKYYLEDGAAATENILLAA
                     WAHEIGSCWVAGDKKEYCEEIRTLLGVPNGFRLVSLVALGYPDQPPAEKKKRSLDDVL
                     HWERW"
     gene            3219..>3615
                     /locus_tag="ENN56_00790"
     CDS             3219..>3615
                     /locus_tag="ENN56_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74053.1"
                     /translation="MTSRERLLTAIHRGTPDRVPIGPYTLGRLDFDAPFTREFIRAVD
                     PLVDTGCGGNMIWGQSAPFEKLPLEHVRDQVVEVIVLHTPKGNLLRKTRRTKIMTSQT
                     EFFCKTPEDAEKVLSVPFTPPSFNLKEYFR"
BASE COUNT          786 a         1046 c         1079 g          704 t
ORIGIN      
        1 ataccaaccc gggccgcgat agaagcgctg ctctggaata aaagaatcgg cggcgttgaa
       61 gcagtgaggg acgctcaccg tctcccaatt tgcgtcgtcg aatttcggct gccacgcgtc
      121 tggcgcctcg tctcgcaaga acttccatcc gtgtgcgagc tcgatccatt cggctttcat
      181 cgtcgacctt tccgcaatgt acctgaagtc atttccgccg tcgtacacaa atatgcaatg
      241 cccggacgtt tactcaagtg agaacgcgat acgaaatcgt tgcgaaggga aaccaggccc
      301 ccgcacgtca ttcgttcgat actcactcca atgttcatct atgtagtatg cagacgccgg
      361 tccattgacc acaacggagg tcattcatga aacgttcatt tgtttctctc atcgcaatag
      421 ccgccgcagg gctcttcgcg gtaaccgcca acgcgcaacc atcacctggc atgggggcgg
      481 gttcgaattc gaatccgggc atctgtgatt ccagcggacc acacggtcct cagatgggtg
      541 gcggcggtgg ccacatgggg caacgcggca acgcgaaggt aatgcttcat cggatctttg
      601 cgcttgatct gacgtcggaa caggcgacgc gggtacgcgc gattcagcag aacgtcgaaa
      661 agcagatcat tccgaagcgt gcgacgcttg atgtgaaacg catcgagctg cgtgagctta
      721 tgcatcagac aaacgtcaat cagcgcgcgg tgacaaacaa gctgaaggag atcaacgacg
      781 tacgcggtga gatcgcacag ctccgcgtaa atgcccaact cgaagctcgt ggcgtcctaa
      841 ctggtgagca gctcgagcgg tacctcgacg cgtcatggcg tccggaaaca tcccagtgtc
      901 ggacaaacgg accggcacgt cgtcgataga tcagtagcac atgcgtactc atcactcggc
      961 acggggcgat ctttgtaagg tcgccccgtg ttcgttgtcg attacccatc cgaatgtcgg
     1021 agacgctatg aacccattcc gccgcgtcat tccattcgcg tttgccgtct gcgttgccgt
     1081 tgtttcgatt gcagtcgcgg gctcacgctc gccggacgcc acgcttgaac cagaaaccga
     1141 cgataatcct cccgcttttt tcggcgccgg gcaatatgcc ccgcaagcgc tgctctttcg
     1201 gatgaaccgt ctcgacctcc gtcccgacca gcgcgacgac atccgtgcaa ttctcacagt
     1261 agcccgtgaa tcggcgacgc cactatcgac ggtgatccgc gaacgggaag ccgcgctgac
     1321 cgaacggctc ctcgccgatg tgatcgatcg tgacggcgtt cgcgccgatc ttgagtcgat
     1381 cggtgtaatg cgtgacgaaa tgaacgcgct gtggttggat gcatacttca atgcgtggga
     1441 acgacttgac gacgaccagc gagccaatct ccgcgagatc gcaaaggaac cggtgcaact
     1501 cgatcgacga cgatcacccg atgcggaccg aagcggtcgc ccgagaggcc cgcgccccgg
     1561 tgacggcatt ggtcgtccag gtggcttcgg aggctggtaa ttccagttcg gtagtacact
     1621 ttcgttgtga tcggggtata ttttcgttgg gggggaccga taggtgtaca ccattctcac
     1681 aggacggaga ttacccatga gagttgcagc acttgtcaca gcgtgcgtga tcggcgcaac
     1741 agcagtgtac ggtgcggatc gccaaccaac gcccgcagcc cggatgagcc aagcggatta
     1801 gcacagccaa ggcgtcggcg tggggcaggc gctcgatcgt cgccctgagg aagccgcttc
     1861 gatgatgctc gaccggatcg gtcaattgcc gttgacatcg gagcagcgtg aagagttgcg
     1921 aacgcttcgg gcgcgcgcgg agcgcgatgt cgccccgctg caggagcagg tgcgtgaaaa
     1981 ggaacagcag ctctacgatc tgcttgtcga aggtgaagtc agcgaagccg aagtgcagaa
     2041 acaggtggac gagatcgcag ggcttcggaa acaggtcgcg atgcggtatg tgaactgcta
     2101 ccggaacatg aacgggcagc tttccaccga tcaacgcaca cgtcttcgtc ggatgagtcg
     2161 cgagcaggac gatgccgaac ggggcaacat gcaaggcgcg ggtcgaagcc agagtgaacg
     2221 cccgggacaa gccggcggcg cgggatcggc cgcgagtggt cgcggaagtg gtgcgggtgc
     2281 tgatgagtca ggtgcgccga caactcagca acagacggat gatgaggcgg aaggcgcaat
     2341 cgagacgccg gaacccgtcg acgaggcgac cggcggcgca ggcggcagtc aatcgggaac
     2401 gcgaggacgt ggacgccggt agaaacacca cactcggtga aacaatcaga cggagtcggt
     2461 tcatgtgaac cggctccgtt tttttcgtta tgcacgtatc gacacgcttt cgaggctttc
     2521 tccaactgtg ggaatatcgc tatcgttcac aatgacatgc gccgtgacac cgaacgtacg
     2581 gaggtatgcc gctatgaacg atccgattac cgtattgaag acccgccgca gcgtccgcag
     2641 tttccggacg acaccgattc cccaagagac gctcgaagac atcatcgatt gcgcgcgtct
     2701 cgcgccgacc ggacgtggcg tccagccgtg ggagttcatt gtcgtcacag acgccgacac
     2761 gcgaaaaggg attgccgaaa tcgccgacta tggctcgttt atcgccgatg cgcccgcatg
     2821 cgtcgccgta ttctgcgcgg atacgaagta ttacctcgaa gacggcgcgg cggcgacaga
     2881 gaacattctc cttgcggcat gggcgcacga gatcggttcg tgctgggtcg ccggtgacaa
     2941 gaaggagtac tgcgaggaga ttcgcacgct ccttggtgta ccgaacggtt tcaggctcgt
     3001 atcgctcgtc gcgctcgggt atcccgatca accgccggcc gagaagaaga agcgttcact
     3061 cgacgacgtg cttcattggg aacgctggta acacgcgtca cacgcgcatc gttgatggcg
     3121 tgcgtcggtg actcgaaggg agcggcattg catccgcgac gtcgctctct gaccttctct
     3181 ccatcccttt cgttcagcct ctcatcggag aatgatctat gacatcccgc gagcgactcc
     3241 tcacggcaat tcatcgtgga acaccggacc gtgtgccgat tggtccgtat acgctcggcc
     3301 gtctcgattt cgatgcgccg tttacccgcg agttcatacg cgcggtcgac ccactcgtcg
     3361 ataccgggtg cggtggaaac atgatatggg gacaatccgc gccgttcgag aagcttccgc
     3421 tcgaacatgt tcgcgatcag gtcgtggaag tgatcgtgct ccacacaccg aagggcaatc
     3481 ttctccggaa gactcgcaga acaaagatca tgacctcgca gacggagttt ttctgtaaaa
     3541 caccggaaga cgcagagaaa gtactttcgg ttccgttcac gccgcccagc ttcaacctca
     3601 aggaatactt tcgac
//
LOCUS       DSCC01000068            1922 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040677, whole genome shotgun sequence.
ACCESSION   DSCC01000068 DSCC01000000
VERSION     DSCC01000068.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040677"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..274)
                     /locus_tag="ENN56_00795"
     CDS             complement(<1..274)
                     /locus_tag="ENN56_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74054.1"
                     /translation="MSEYPHIPIHNPKPDATAFIDYLLGRRETHTRAPMVEYLVDDLL
                     RKHIGTEMLGQTWTDGSGGRADRDRFFANFIDFWYRMGYDYVRYEQG"
     gene            complement(295..1704)
                     /locus_tag="ENN56_00800"
     CDS             complement(295..1704)
                     /locus_tag="ENN56_00800"
                     /inference="COORDINATES: protein motif:HMM:PF04734.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74055.1"
                     /translation="MNPRFPLLAGSAQIDITPEPGAQLSGNVAVREPAESVLDPLYAK
                     ALVVKNDETALCVIQLDAIFIERQYTEHIRAEIERRCGIPRDAIMVHTTQTHAAPSIR
                     GFLLDVDFELPEEFDWLRLSDPVFAERAINGAIEAAARAHAELRPARIGWNTAIVDGI
                     AFNRRGIRRDGTCGMPWVYTSVERPLGPTDYRYVEGPTDPEVGVVWLQDEHMQSIGFL
                     LHFTCHPVNVFYHRGRGRHSQISADWPGAWATMLTQRHGGVGLVLNGCCGNINPWPAF
                     TPNFVPDHLRMGERLTAMSSEAVGRITFPNETPKLAFSTRDIPLVIREPDAEALRKAK
                     QRIAEQPNPQMISDTRVDHEWFYAAMYVSLDMLRQRTGGTEPYEIQTLRLGDVAVVGL
                     PGEPFVEGQLKLKIDSPTFPTLVAHATTQYAGYIPTREAFARGGHECNPSWWARREPG
                     ALETIVETATEMLNGLFAK"
     gene            1710..>1922
                     /locus_tag="ENN56_00805"
     CDS             1710..>1922
                     /locus_tag="ENN56_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74056.1"
                     /translation="MLLLCIPDPDVWQILQRANDHRRLFDGQCESVVTDIERALHKKR
                     FSAIAQIERAERFPINNDGYPSEAVPA"
BASE COUNT          353 a          574 c          605 g          390 t
ORIGIN      
        1 caccctgctc gtagcgcacg tagtcgtagc ccatgcggta ccagaaatcg atgaagttcg
       61 cgaagaagcg gtcgcgatcg gcgcggccgc cgcttccgtc ggtccatgtc tgcccgagca
      121 tttccgtgcc gatgtgcttc cgcagcaagt catcgacgag gtactcgacc atcggcgcac
      181 gggtgtgcgt ttcgcgtcgc ccaaggaggt agtcgataaa cgcggttgcg tcgggtttcg
      241 gattatgtat tgggatgtgc ggatattcgg acatagatac ctcgtatgac ggaatcactt
      301 cgcgaaaagt ccgttgagca tctccgttgc agtttcgacg atggtctcga gtgcgcccgg
      361 ttcacgtcgc gcccaccacg acgggttgca ttcgtgccca ccacgtgcga acgcttcgcg
      421 cgtcgggata taccccgcgt attgcgtggt ggcgtgggcg acgagcgtcg ggaacgtcgg
      481 cgagtcgatc ttcagcttga gctgtccctc gacgaatggc tcgcccggaa gtccgaccac
      541 cgcgacatcg ccaagtcgca acgtctgaat ctcatacggc tcggtgccac cggtgcgctg
      601 cctgagcatg tcgaggctca cgtacatcgc cgcgtaaaac cactcgtgat cgacgcgagt
      661 atccgagatc atctgcgggt tgggctgctc ggcgatgcgt tgtttcgctt tgcggagcgc
      721 ttcggcatcc ggttcgcgga tgacgagtgg gatatcgcgc gtcgagaagg cgagttttgg
      781 cgtttcgttg gggaatgtga tccgtccgac ggcttccgaa gacatcgcgg taaggcgctc
      841 gcccatgcgg aggtgatcgg gtacgaaatt cggcgtgaat gcaggccaag ggttgatgtt
      901 cccgcagcat ccgttcagca cgagaccgac gccgccgtgt cgttgggtaa gcatcgtcgc
      961 ccatgcgccc ggccaatcgg cgctgatctg ggaatgccga ccgcgtccgc ggtggtagaa
     1021 cacgttcacc gggtgacagg tgaaatgaag gaggaacccg atgctctgca tgtgctcgtc
     1081 ctgcagccag acgacaccaa cctccgggtc cgtcgggcct tcgacgtatc ggtaatcggt
     1141 cggaccgagc gggcgttcga cgctcgtgta cacccacggc atcccacacg tcccgtcacg
     1201 tcggataccg cggcgattga acgctatccc gtcgacaatt gccgtgttcc agccgatacg
     1261 cgccgggcgg agctccgcat gtgcgcgagc cgccgcttcg attgcgccat tgatggcccg
     1321 ctcggcgaac acgggatcac tcagccggag ccagtcgaac tcctccggaa gctcgaagtc
     1381 tacgtcgagg agaaatccgc ggatcgacgg cgcggcgtga gtctgcgtcg tatgcaccat
     1441 gattgcgtcg cgcggaatcc cacaacgtcg ctcgatttcc gcgcggatat gttcggtgta
     1501 ctgccgttcg ataaagatcg cgtcgagctg gatgacgcag agagcggttt cgtcgttttt
     1561 cacgacgagc gccttcgcgt acagcggatc gagcaccgat tccgcgggct ccctgactgc
     1621 gacattcccg gaaagctgcg cacccggctc cggcgtgatg tcgatctgtg ccgatccggc
     1681 aagtagtggg aaacgtggat tcatggggtg tgctcctctt gtgtattccc gatccggatg
     1741 tctggcagat actccaacga gctaatgatc accggcggct gttcgacggt caatgcgaga
     1801 gcgtggtaac cgatatcgag cgcgcgctcc acaagaagcg gttcagtgcc atcgctcaga
     1861 tcgaacgagc cgagaggttt cccatcaaca acgatggata cccgtccgaa gccgttcccg
     1921 cg
//
LOCUS       DSCC01000069            1781 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045099, whole genome shotgun sequence.
ACCESSION   DSCC01000069 DSCC01000000
VERSION     DSCC01000069.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045099"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            25..1047
                     /locus_tag="ENN56_00810"
     CDS             25..1047
                     /locus_tag="ENN56_00810"
                     /inference="COORDINATES: protein motif:HMM:PF00293.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDS74057.1"
                     /translation="MSSAMSSRYPTRPNARSGMIPAGIMKYTTASATMIFGATGIRRS
                     HNSRQTIIGSLNHMSSVLIDRASSSKDTRSFVLLSDQYHGNGCVKYVVVASMYHFHRI
                     PTNRRVTTIPWRISFALVRAYGVATSLTPRDLTGTLCRCAKIGSTALRDDSMTSDIIT
                     RESGSSYVVDLSEVGPEEHDRFIGTPGEVILVLTTRDELVWLHTKDFYPNGIYRLPTG
                     KLHEGESPDAGFARELHEEAGIAPIPPPECIAHIRYQSGDEVYPFVSYVYRVHDANDS
                     PSPIDESEQITGWRCVPVRELTSVATELRTLPKRWASWGRFRAPAHDVIAEVLRGNSI
                     EDKGRT"
     gene            1044..1697
                     /locus_tag="ENN56_00815"
     CDS             1044..1697
                     /locus_tag="ENN56_00815"
                     /inference="COORDINATES: protein motif:HMM:PF03737.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HDS74058.1"
                     /translation="MKNDHDLDMLAREAYTAVFCDVLDDMGYRSQSTNGDVRPLWPDA
                     RLVGYARTVRVEAVEQASESPYEKEFTLVDALNPGDIVVAECGTTRSAFWGELLATAA
                     QVRGARGALIDGYCRDVFKVRDLSFPVFARGTAPTDSKGRTEVAETDIPITFDGVRVA
                     TGDLVFGDFDGIVIVPRDVADEAITKALEKVHGERTVFEALRNGMSAREAWERWGIL"
BASE COUNT          409 a          499 c          534 g          339 t
ORIGIN      
        1 atcatgaacc acgcgatcgc gagaatgagc agtgcgatgt cgtcgagata cccaacaaga
       61 ccgaacgcgc gatccggaat gatccccgcc gggataatga aatacacgac ggcgagcgca
      121 acgatgatct tcggcgccac gggcattcgc cgatcccaca acagccgcca gacgatcatc
      181 ggaagcttga accacatgag tagtgttctc atcgatagag cttcatcctc gaaagataca
      241 cgctcgttcg tcctcctcag cgaccaatac cacggcaatg gctgcgtgaa gtacgtcgtc
      301 gtcgcttcaa tgtaccattt tcaccggata ccgacaaatc gccgcgtaac cacgatacca
      361 tggcgcatat cgttcgctct cgtgcgtgcg tacggcgtgg cgacgtcgtt gactccgcga
      421 gatttgactg gtaccctgtg taggtgtgca aaaatcggtt ctacagcgct tcgagacgac
      481 tcgatgacct ccgatatcat cactcgcgag tcaggtagtt cttacgtcgt cgatctcagc
      541 gaggtcggac ctgaagaaca cgatcggttc attggcacgc cgggcgaggt gatcctcgta
      601 ctcaccacgc gcgatgagtt ggtctggctg catacgaagg atttctatcc caacggcatc
      661 tatcgattgc cgacggggaa gcttcatgaa ggtgaatcgc cggacgccgg ctttgcgcgt
      721 gagcttcatg aggaggcggg tatcgcaccg ataccaccgc ccgaatgtat cgcgcatatt
      781 cggtaccagt caggcgacga agtatacccc tttgtgagct atgtgtaccg cgtgcacgat
      841 gcaaacgatt cgccgtcgcc gatcgatgaa tcggagcaaa tcacgggttg gcgctgcgtg
      901 ccggtgcggg agcttacgtc agtagccacg gagcttcgca cgctgccgaa acggtgggca
      961 tcatgggggc gattccgggc gcccgctcat gacgtaatcg ccgaagtgtt gcgtggaaac
     1021 tcgatcgaag ataaaggacg aacatgaaga acgatcacga cctcgatatg ctcgcgcgcg
     1081 aagcgtatac cgccgtattc tgcgatgtgt tggacgatat gggctaccgg tcccaatcga
     1141 cgaacggcga cgttcgaccg ctgtggcccg atgcccgatt agtggggtac gcgagaacag
     1201 tgcgtgtcga agcggtggag caggcatccg aaagcccgta cgagaaggaa ttcacgctcg
     1261 tcgatgcgtt gaatccgggt gacatcgtcg ttgcggaatg cgggacgacg cgctcggcat
     1321 tctggggcga actgctggcg accgcggcac aggtacgcgg tgcacgcggc gcgcttatcg
     1381 acgggtactg tcgcgacgtg ttcaaagtgc gcgatctctc gtttcccgtt ttcgcccgtg
     1441 gtactgcgcc gaccgattcc aaaggccgca cggaagtcgc tgaaacggac attccgatca
     1501 ccttcgacgg cgtccgcgtg gctaccggtg acctcgtatt cggcgatttc gacggcatcg
     1561 tcatcgttcc gcgcgacgta gccgacgagg caatcacgaa agcgctcgag aaagtgcacg
     1621 gcgagcggac ggtattcgaa gcgctgagaa atggcatgtc ggcgcgcgaa gcgtgggaac
     1681 gctggggcat cctgtagtca gcaatacaac ggcacaataa gagaaaaacg aacggagtaa
     1741 cacgatgaca gacggaaaag acacgacaca cgaacaacgc g
//
LOCUS       DSCC01000070            3570 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017632, whole genome shotgun sequence.
ACCESSION   DSCC01000070 DSCC01000000
VERSION     DSCC01000070.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017632"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..278)
                     /gene="folK"
                     /locus_tag="ENN56_00820"
     CDS             complement(<1..278)
                     /gene="folK"
                     /locus_tag="ENN56_00820"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:PF01288.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDS74059.1"
                     /translation="MSSALIGLGSNLGDRIQYLRRACEQLGTHPAVRIAATSPLYETP
                     PIGPPQGTFLNAVIRVETSLSPYGLLRLCQAVERAGKRKRTIHWGPRT"
     gene            complement(275..694)
                     /gene="folB"
                     /locus_tag="ENN56_00825"
     CDS             complement(275..694)
                     /gene="folB"
                     /locus_tag="ENN56_00825"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:PF02152.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDS74060.1"
                     /translation="MPNEKSPPSKTASNASDHSARDRVRLVGMQFYGYHGATPQERQI
                     GQRFEVDVEMAVDTEKIVDGDLGTTIDYRIVYEICRRCCAELQYRLIETMAREIAREI
                     LRAHDLPGVRVTVRKPCVPLGGVISVGAEATVERGEI"
     gene            complement(808..1485)
                     /locus_tag="ENN56_00830"
     CDS             complement(808..1485)
                     /locus_tag="ENN56_00830"
                     /inference="COORDINATES: protein motif:HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HDS74061.1"
                     /translation="MEVAHHYAGWMGDDDYASRHERRRALDILLSDRKNIPVDSVFLR
                     AIRWLENPGTDSDIALRSEWPNHFIGGQDDEVMFLAYDDLDGDGLDDIIVPTRNGPIL
                     VFQRPADTPTDWNLTRIPMPLNTGTGKSAAIGDVNNDGVNDIVVTCENARDGRIGVFY
                     LTRTSTTDETWTPHNIGGPTGVKYDLVRLIDLDEDGDLDALTCEETEGLGVIWYENPT
                     ISRGAAR"
     gene            complement(1311..2087)
                     /locus_tag="ENN56_00835"
     CDS             complement(1311..2087)
                     /locus_tag="ENN56_00835"
                     /inference="COORDINATES: protein motif:HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HDS74062.1"
                     /translation="MRMRTLLRTTTLALTVALSGIGCASATGENEVVGEPWKMHVIDS
                     TSHGADGVRLADFDSDGLLDIVTAWEEGGCIRIYRNPGPTHVHEPWPSVTVGAVDSPE
                     DAFFVDLDNDGNLDVVSLTEGSDRTVYVHWAPSDPAQYLTADAWQTEPVPDSRGRMQW
                     MFGFPLALRDTTAIDVMLTAKNTGAELGWYEVADVPRDLAAWKWHTITPVGWAMTIMP
                     VDMNGDGRSISCSPTERTFRWIPYSFARSDGWRTPVPTQI"
     gene            complement(2128..>3570)
                     /locus_tag="ENN56_00840"
     CDS             complement(2128..>3570)
                     /locus_tag="ENN56_00840"
                     /inference="COORDINATES: protein motif:HMM:PF16126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HDS74063.1"
                     /translation="LNTLLVQASSFDVRDVEAHLVPEATKRGIHIEWSHHNMGTWLPW
                     DRYGVKHPEYYAVRDAVRTNDRAQQLCLCTSNPAVANEVARNIAQFCRDHPWAHTVGL
                     WPNDGYGSCECDDCATLDRYSDEESREVAYYPGTDEAIPLTAVDRNKTNRYVRFLNDV
                     AERLVAEQPDARLSALFYVDVVRPAPDEPLHPAIDPMIALYWRCSAHALFDESCPTNQ
                     WFASVVREWLDYAPGRVSFYEYYMGMDEYASLPFPIVDALRTDWQAYAGGEDGVRGIV
                     GASIQSAASHHTAYGLNYALFAALAWDPNTDVDTFLDRWLEASYGPAARVAGEWWSAL
                     MNQMSDIAAGEGPDTHDANRPSCYTPVRLNFPALWDETGMARIGGMLDGAIDLPGMSH
                     GQSARLDDLRTYHVYCVASNEAYRRELEARQYEANGGVLNDRKVALARAVVEGLDHIA
                     EFTEQIDAQTIIFTKRIRHRLATFRRMWTK"
BASE COUNT          642 a         1057 c         1089 g          782 t
ORIGIN      
        1 attgtgcgcg gtccccaatg tatcgttctt tttcgctttc cggcgcgttc gacggcttga
       61 cagagccgga ggagaccgta cggtgacaac gatgtttcaa cgcgaatcac agcattgaga
      121 aacgttcctt gcggcggtcc gattggtggc gtttcataga gcggtgatgt cgctgcaatc
      181 cggacggccg gatgcgtgcc aagctgttca catgcgcgtc gtaggtattg gatgcggtcg
      241 ccgaggttcg atcccagtcc gatcagcgca ctgctcatat ctcaccgcgt tcgacggtcg
      301 cttccgcgcc gaccgaaatc actcctccga ggggaacgca cggctttcgc actgtcacgc
      361 ggactcccgg gagatcgtgc gcgcgtagaa tctcgcgtgc gatttcccgc gccatggttt
      421 cgataaggcg atactgcagt tccgcgcaac accggcgaca tatctcgtag acaatgcggt
      481 aatctatcgt cgtgccgagg tcgccatcga cgattttctc cgtatccacc gccatttcga
      541 cgtcgacctc gaaccgctgt ccgatctgac gttcctgcgg agtcgccccg tggtacccgt
      601 agaactgcat tccgacgagg cggacgcggt cacgtgccga gtgatcggaa gcattcgatg
      661 cggttttcga tgggggagat ttctcgtttg gcattcgcta cgggcgctcc atgtcattcg
      721 atttgcgagg atggcagatc aggtatcggt aatcaacaaa catacaaggt agcagagtct
      781 ttcgcgcggt ccgagtggcg actcccatta tctcgctgca ccacgtgaga ttgtcgggtt
      841 ttcgtaccag ataacgccaa gtccttcggt ctcttcacac gtgagagcgt cgaggtctcc
      901 gtcttcgtcg aggtcgatca ggcggacgag atcgtatttc actccggtgg gaccgccgat
      961 gttgtgtgga gtccacgttt catccgtcgt tgatgttcgc gtcagataga acacgccaat
     1021 gcgcccgtcg cgtgcgttct cacacgttac gacgatgtcg ttgacgccgt cgttgtttac
     1081 gtcgccgatg gcggcggact tgcctgtgcc ggtgttcagc ggcatgggta tccgagttag
     1141 gttccagtca gtcggtgtgt cggcaggtcg ttggaagacg agtatcggcc catttcgcgt
     1201 cggaacgatg atatcgtcga ggccgtcgcc gtcgaggtcg tcgtatgcga ggaacatcac
     1261 ctcgtcgtcc tgcccaccga tgaagtgatt cggccattcg cttcggagcg ctatatctga
     1321 gtcggtaccg gggttctcca gccatcggat cgcgcgaagg aatacggaat ccaccggaat
     1381 gttctttcgg tcggagagca ggatatcgag cgcccgtcgc cgttcatgtc gactggcata
     1441 atcgtcatcg cccatccaac cggcgtaatg gtgtgccact tccatgcggc gaggtcgcgt
     1501 ggaacatcgg cgacctcata ccatccgagc tccgcgccgg tgtttttcgc ggtaagcatc
     1561 acgtctattg ctgtggtgtc tcgaagcgcg agtgggaatc cgaacatcca ctgcatacgt
     1621 ccgcgtgaat cgggaaccgg ttcggtttgc cacgcatcgg cggtgaggta ctgcgctggg
     1681 tccgatggtg cccagtgcac gtacaccgtg cggtcggagc cctcggtcag gctcacaacg
     1741 tcgagattgc cgtcgttgtc gaggtcgacg aaaaacgcgt cctccggact gtcaacggcg
     1801 ccaacggtga cactcggcca tggctcgtgt acgtgcgtcg gaccgggatt gcggtagatg
     1861 cggatgcagc cgccttcctc ccatgcggtg acgatgtcaa gcaggccgtc gctgtcgaaa
     1921 tcggcaagtc gcacaccgtc ggcaccgtgc gatgtactgt cgattacatg cattttccac
     1981 ggctcgccga cgacttcgtt ttcgccggtt gccgatgcgc acccgatgcc ggagagtgcg
     2041 acggtgagtg cgagtgtggt ggtgcgaaga agcgtgcgca tacgcatgga ggcctcgtgt
     2101 tcgttcgttc aagtagctcg cctgcgctta ctttgtccac atcctgcgga aggtagcgag
     2161 acggtggcgg atacgcttcg tgaagatgat cgtctgtgca tcgatttgct cggtgaactc
     2221 ggcgatatga tcgagacctt cgacaacggc acgagcgagc gccaccttcc gatcgttcag
     2281 cacaccgccg ttcgcttcgt actgccgcgc ttcgagttcg cggcgatacg cctcgttgct
     2341 cgcgacgcag tagacgtggt acgtgcgcaa gtcgtcgagc cgcgccgatt gcccgtgcga
     2401 cattccgggg aggtcgattg cgccgtcgag catgccgccg atacgcgcca taccggtttc
     2461 gtcccagagc gccgggaaat tgagtcgcac gggagtgtag catgagggac ggttcgcatc
     2521 gtgtgtgtcc ggtccttctc cggcggcgat atcgctcatc tgattcataa gcgcactcca
     2581 ccattcgccg gcgacacgtg cggcgggtcc gtaactcgct tcgagccaac ggtcgagaaa
     2641 cgtatcgacg tcggtgtttg gatcccacgc aagcgcagcg aaaagcgcgt agttcagacc
     2701 gtacgccgtg tggtggctcg ccgccgattg aatactcgcg ccgacaattc cacgcacgcc
     2761 atcttctccg cccgcgtacg cctgccaatc agttcttagc gcgtcaacga tcggaaacgg
     2821 tagcgacgcg tattcgtcca tgcccatgta gtactcgtag aaggacacgc gacccggtgc
     2881 gtaatccagc cattcccgca cgaccgacgc gaaccactgg ttcgtcgggc acgattcgtc
     2941 gaacagcgcg tgcgcggaac accgccagta gagcgcgatc atcggatcga tcgcggggtg
     3001 gagcggttcg tcgggcgcag gccgaacgac atcgacgtag aacagcgcgc tcaatcgcgc
     3061 atccggttgc tctgcgacga ggcgttcggc gacatcgttg agaaagcgca cgtaccgatt
     3121 cgtcttgttc cggtcgactg ccgtgagcgg aatcgcttcg tcggttcccg gataatatgc
     3181 gacctctcgg ctttcctcat cgctgtaccg atcgagtgtt gcgcagtcgt cgcactcgca
     3241 tgagccgtac ccgtcgttcg gccagagtcc cacggtatgc gcccatgggt gatcccggca
     3301 aaactgcgca atgttccgcg cgacttcatt cgcaacggcc gggtttgacg tgcacaggca
     3361 gagttgctgc gcgcggtcat tcgtacggac agcatcgcgc accgcgtaat actccggatg
     3421 cttcacgccg tatcgatccc acggtagcca cgtgcccatg ttgtggtgcg accattcgat
     3481 atggatgccg cgtttcgtcg cctcgggaac gaggtgcgct tcgacatcgc gtacgtcgaa
     3541 cgagctcgcc tgcacgagga gcgtgttgag
//
LOCUS       DSCC01000071            1944 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040004, whole genome shotgun sequence.
ACCESSION   DSCC01000071 DSCC01000000
VERSION     DSCC01000071.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040004"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1761
                     /locus_tag="ENN56_00845"
     CDS             <1..1761
                     /locus_tag="ENN56_00845"
                     /inference="COORDINATES: protein motif:HMM:PF16126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HDS74064.1"
                     /translation="HPEWFALQPDGTRSDQLCTTNPDVLDSAVAVVSRYFDTRPDAPM
                     YSLSPNDNDDFCQCERCRAFDTEIGVNPVGANGSYTDRLVYFFNQIAERLEDDHPDKQ
                     LAFYAYINHTAPPETVKPHRMLLPVACHTPWDYCQNHAIDDPNCRRNARFAATMQGWE
                     KLTDETYVYDYYGSFAWFGPMGIVHAIRRDLPWLHAHGVTGFNSETHANWWSQGLNWY
                     VASKLFWDLDADVDQIVTDWFTHLYGPAAPQMTAFWAMYEDTAQNTPYNRDAERGWVM
                     SMDREFFARTDSLLNAAESAVATSSISSTDSADIRERIRKMRFADRVAQAQAPFEIFK
                     ANNGDIYPVLAAKVDPMDVLAEYDADSSLHDVMDGSLGRGSLMGAKAGLKNIRDVWDA
                     TNLSEERREEILRMLAEGADTEAARALGFITNWKVAGFFYAPTAQGLRYPFPPERELD
                     LATSYPTSNGDIAWRDYTVENAFGVINFRDLYPDQSVNYAVAYAHAEVEYYGRKSRGT
                     IRLGSNDGVAVWHNGELVLVSDVSRPCNPDQDRIMVPLSRGTNRFLVKVFNTQNNFKM
                     TMRVFDDAERPIMQDTGLPE"
     gene            complement(1783..>1944)
                     /locus_tag="ENN56_00850"
     CDS             complement(1783..>1944)
                     /locus_tag="ENN56_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS74065.1"
                     /translation="EDKELHAVWIENTPMRRTGDPEELAGLALYLASDASSYMTGSDV
                     IIDGGYHVW"
BASE COUNT          420 a          561 c          584 g          379 t
ORIGIN      
        1 catccggagt ggttcgcgtt gcaaccggac ggcacccgat ccgatcaact ttgcacaacg
       61 aaccccgacg tgctggattc ggcggtcgca gtcgtttcac ggtatttcga cacgcgaccg
      121 gacgcgccga tgtattcgct cagcccgaac gacaacgatg atttttgcca atgcgaacga
      181 tgccgcgcat tcgatacgga gatcggcgtc aatccggtcg gcgcgaatgg gagctatacc
      241 gaccggctcg tgtatttctt caatcagatc gccgagcgcc tcgaagacga tcatccggat
      301 aagcagctcg cgttctacgc gtacatcaac cacaccgccc cgccggaaac cgtaaagccg
      361 catcgaatgc tgctgccggt ggcgtgtcac acgccgtggg actactgcca gaaccacgcg
      421 atagacgatc cgaattgccg ccggaacgcg cgattcgccg caacgatgca gggatgggag
      481 aagctcacgg atgagaccta tgtgtacgac tactacggct cgttcgcatg gttcggaccg
      541 atgggtatcg tgcacgcgat tcgtcgtgac ctgccatggc tgcacgcaca cggtgttacc
      601 ggattcaaca gcgagacgca cgccaactgg tggtcgcagg gattgaactg gtacgtcgcg
      661 agcaagctct tttgggatct cgacgccgac gtggatcaga tcgtcacgga ttggttcacc
      721 catctctacg gacccgcggc gccgcagatg acggcgttct gggcgatgta cgaggatacc
      781 gcgcagaaca cgccgtacaa ccgcgatgcc gagcgcggct gggttatgtc gatggaccgg
      841 gagttcttcg cgcgtaccga ttcgctactc aatgcagcgg aatcagcggt tgcaacgagc
      901 agcatcagct ccaccgatag tgcggatatc cgggagcgga ttcgcaagat gcggtttgcc
      961 gatcgcgtcg cgcaggcgca ggcaccgttc gagattttca aggcgaataa cggcgacatc
     1021 tatcccgtgc tcgcggcgaa ggtcgatcca atggatgttc tcgcggaata cgacgcagat
     1081 tcatcgctac acgatgtgat ggacgggtca ctcggtcgtg gttcgttgat gggagcaaag
     1141 gctgggctga aaaacatccg cgacgtgtgg gacgccacga acctcagcga ggagcgccgc
     1201 gaggagatac tccgtatgct cgccgagggc gcggatacgg aggcggcccg cgcgctcgga
     1261 ttcatcacta attggaaagt tgccggattc ttttacgcac cgacggcaca gggtttgcgc
     1321 tatccgtttc cgccggaacg cgaactcgat cttgccacat cgtacccgac atcaaacggc
     1381 gatatcgcgt ggcgcgatta cactgtagag aacgcgttcg gtgtgataaa ctttcgcgat
     1441 ctctatcccg atcagagtgt gaactacgcg gtggcgtacg cacacgccga ggtcgaatat
     1501 tacgggcgga aatcacgtgg cacgatacga ctcggatcga acgacggtgt cgcagtatgg
     1561 cacaacggcg aactcgtctt ggtgagcgac gtatcgcggc cgtgtaatcc cgatcaggac
     1621 cgaatcatgg tgccgctttc gcgcggtacg aatcggttcc ttgtgaaggt gttcaacacg
     1681 cagaacaact tcaaaatgac catgcgcgtg ttcgacgatg cggaacgccc aatcatgcag
     1741 gataccggtc tgccggaata gcggtgcatc taagacgacg ggtcaccaga cgtggtaccc
     1801 gccgtcgata atcacatccg agccggtcat gtaactcgac gcgtccgatg cgagatacaa
     1861 cgcaagcccg gcgagctctt ccggatcacc ggtgcggcgc atcggcgtgt tttctatcca
     1921 cacggcgtgg agttccttgt cttc
//
LOCUS       DSCC01000072            2275 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032293, whole genome shotgun sequence.
ACCESSION   DSCC01000072 DSCC01000000
VERSION     DSCC01000072.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032293"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
BASE COUNT          467 a          690 c          619 g          499 t
ORIGIN      
        1 aatattcggc gttcgcgttg ataccgcgcc acccacgaac cgccccgcgc catgtatgtg
       61 cgatcgaccg ttgtcgatcc tcccaatccg taagcgtcgt atcccacagt tggtcggcac
      121 gttgtccgac tgatacagac gcagcaaact gctcgctgcc cgtcgacgcg atctcggcga
      181 catattcgcc aaatcgagat ccgcgaatac gtgatccgtc ttcttcggat tgatcaagct
      241 ctccgtcgat acgcacactt ggtcgtatgt acccaaccgc gtgtcgtacg aatccgtttc
      301 ctcgatagat ctcagattca acgggtgcat ctgtggaacc gcgcgattcc gaacgaactg
      361 cctcgagata cgcacgcact tccggcgaca cccgtccgac gctttcccaa tcagcaccca
      421 ctcgtcgcgc cgtataccct gatgacgtgg ccgtcgaatc tcgatcgaac aaaccagcat
      481 cgactgtaag cttgtggtcg ggactgggtg aataaacgaa cgagacttct ccggtacgct
      541 cctgatcgcg ccgatctgat aacgaaagcc cccatcgacg attgtcctcg gccggtgtcg
      601 tacgcgacaa cgcggtgaat cccttgtcga cacgacggcc agtgccggtg atcgcgactg
      661 ttccggcctt gccgagaggt acttcgtcgg tgcgaattgt cgcccgaccg gcccaatggc
      721 ggttatcccc atcatcgagc gatgataacg tgttttcgtc gcgatcgctc agcgcaactt
      781 ccacctcggt agtgacgcct gaaatcggca gcgcttgcgc gtgaagtgtt gtcactctgg
      841 tccgatccgg cagcgggagt actacatagg gaacgtagtt accattcccc ggcccaaccc
      901 attggtattc attcgtaaag tacccatcga cccgcgtgta cgtacctttg cccggtgcaa
      961 cttggctgaa ctggatgctg tatcgttcgt cgggcggagt cgtgtcgtcg tcgatccacg
     1021 caaagtgacc gaccgaaagg tcgatagcgc gatatgttcc gttcgggtcc gccacccagc
     1081 ccggtcgcgc ggcggccatc ggatcgtctc cggccccacg gattacatca agcgcactgt
     1141 cgtcgagagg aagtgcgagg ggatcatcga cgttatcacc ttcttgggca tagaataccg
     1201 caagctgcca acggtttgtt tcgcttttca gttcaccggt cattacggcg cttgacctgc
     1261 cgtaatcatc gcctgcgtat tcgtaatcga cgacgatgcg catgtcgtcg gtaatatgtc
     1321 gcgctcgtgt aaacgtgatc tcgcccgcgc cgtaatccat cacgtaatcg ttattctgcc
     1381 cgcgacggag tgcttgaccg ttgagccaca cgcgttccga tccggcgatc acgacaacgt
     1441 taccgtttcc cgcgttgtca acgagccgat acggcccctg aatcccgtca atgacgggca
     1501 gttcgtacgc gcgaaaacgc ccacgtgcgg atgcaacgcc tccggtgact gaccaccggt
     1561 cataccgagc ctgcaggagc gctccttcga gtcgacggtc atatgctccg aattcgccgc
     1621 ttcgatagag gaagtcgtaa ctgccgagta ctgcacggta gtgcggactc ttgatctcta
     1681 caagtacttt atcgatctcg cggatgtcct ctgtcgtccc ctcgggttga atcggtaggt
     1741 tctgatcgct cagctcggcg gtaacgtaga cgtcctcacc gatggtgccg ttgatactca
     1801 cccgcagtga ctgttcgaga gtcatatctc gattcgaccc gacctgtatc gcgaacgtct
     1861 taacaccgcc gatccgaaga tctccggaag gttcgttcgc gcccgagcgt tccgtaacga
     1921 acgtcggcag cgactcggga ttcgagtcac tcggaggttc aacgggcgtc atccaacggt
     1981 acgtcaacgg ccaatcaacg gggagggcgc ggtattcaac acgaatagtc gatcctatgg
     2041 gccacgaatt cgtcagtcga acggtgcgcg tcgtcaagtc aacggtgaaa tcgtgactcg
     2101 gaatgagccg taggtcgtcg acgaagacgt ggatcgaatc gcgtacaagg attcgagttg
     2161 gtagctggat aaccgcgtcc tcagcggttt cgacggtttc agtatgggta aaccatccga
     2221 ccctgatggt ctcggcacgc acacaggtcg ccgatacgag gacaacgaga aacgt
//
LOCUS       DSCC01000073            3529 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017937, whole genome shotgun sequence.
ACCESSION   DSCC01000073 DSCC01000000
VERSION     DSCC01000073.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017937"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..288)
                     /locus_tag="ENN56_00855"
     CDS             complement(<1..288)
                     /locus_tag="ENN56_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74066.1"
                     /translation="MIDQANARIPLDDLDGPRGCQRHEFGELLDFVNFVFRSDQGRRP
                     SMGGDYPHLYNEANCENFRRIRHRGRIVSCVNIYPALIQWGDAVLKIGAIGG"
     gene            complement(392..467)
                     /locus_tag="ENN56_00860"
     tRNA            complement(392..467)
                     /locus_tag="ENN56_00860"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(432..434),aa:Cys,seq:gca)
     gene            542..1357
                     /locus_tag="ENN56_00865"
     CDS             542..1357
                     /locus_tag="ENN56_00865"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDS74067.1"
                     /translation="MCRSTASPSGTSITRGTLTLLRRTNLRVARSRRIHHMIRAVVFD
                     LDNTLVDFMKMKDRAVDAAAWAMVDAGLQMTPEDVKRGIYDQYDKYGIESQDVFDKYL
                     TEILGEIDYRVLAAGIIAYRKAREASLQPYPHVRIIIAKLLRRGVKLAVLSDAPRREA
                     WLRLCQLGLHHEFDVVVTYEDTGYRKPHPEPFARVIDMLGIAPREAVMVGDWPERDVA
                     GAKVAGLWTVWAKYGSVHAVETTNADYEIESVDEIVDVIDAIDARMASNTSEP"
     gene            1405..3222
                     /locus_tag="ENN56_00870"
     CDS             1405..3222
                     /locus_tag="ENN56_00870"
                     /inference="COORDINATES: protein motif:HMM:PF07993.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74068.1"
                     /translation="MTCSVFARTPPSFTKRAEFTEELTFPDRSQIITHRYLLPAPANT
                     NVGQYLFHLSNRTNMNPISEFLEGKTLFITGATGFLAKGLVEKLLRQAPGIRRIYLLI
                     RSSRQTDGRIVTAEQRLKRDIIDSSAFSALRALYKDDYEDFVADKLVPVTGDLTCERL
                     GIGVETYDQLCREVDIIINSAATVVFDERVDLALDMNTLGPRRVLGLAQDCHDAVFAH
                     VSTAYVNGEQIGELPERLLSPTQCIANITRNGAGPEYDLDNEIARIQALRDQVLAEAG
                     STDLTQKFTKMLDRQDAGKRVTEHRREHQLDALRQRWITKRLVNEGIARARELGWHDN
                     YTYTKAMGEQLLVRDHGNVPLVIVRPSIIESSLYEPEPGWLDGLKVADPLIAHFGKGR
                     LPDFPGNPSSILDVIPVDMVVNVIIGALPEARNGNDVKVFHVATSAENPLTFGRIVDL
                     TYDYFKRYPMRDRHGEPIPVRPWHFPRISAFRLRYYLRYRLPVTVMSWAIQHLRFINW
                     SSRLKRRISTLDALSERVLSLVDIYSPYTNLHCTYGIANTRALFQNLSPEDRESLRFD
                     VSQIDWREYLQDIHLPGLKRHVLKMPGERKTSNEHTEEV"
     gene            3222..>3529
                     /locus_tag="ENN56_00875"
     CDS             3222..>3529
                     /locus_tag="ENN56_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74069.1"
                     /translation="MASRSSKIPYHVELLGDRATMYATYDEYVQHFLDVIRMEGYFDS
                     DMGEIVWPTGSVEQGTVGREDLVRRSALVNAVYRGLPPLRDDRLADAWTELERLIPLY
                     "
BASE COUNT          803 a         1030 c          986 g          710 t
ORIGIN      
        1 gccaccgatc gcgccgatct tcaacacggc gtcgccccac tgaatcagcg cgggatagat
       61 gttcacgcaa ctgacgatgc gtccgcgatg acgtattcgg cggaagttct cacagttcgc
      121 ttcgttgtag aggtggggat agtcgccgcc catgctcggg cgccgtccct gatcggagcg
      181 gaagacgaag ttgacgaaat cgaggagttc gccgaattcg tgtcgctgac atccgcgcgg
      241 tccgtcaagg tcgtcgagtg ggatacgcgc attcgcctga tcgatcatga cctgttactc
      301 cacggcaaaa cggttcttgc gtccctgaga aaacaaaaaa aggaccatcg gagcgctttt
      361 gaaaagcccc accgatggtc ccgaaaagga atggaggcga cgggcggatt cgaaccgccg
      421 aatagaggtt ttgcagacct ccgccttggc cacttggcta cgtcgcctca ctctgtcgta
      481 caatctacat gagagaaaaa atgtatgggt acaccacgaa tcaggcaagg gcagcaagca
      541 aatgtgccgt tctacggctt ccccttccgg cacgagtatc acacggggta ccttaacact
      601 cttgcgtcgc acgaatctgc gagtcgcacg cagccggagg attcaccaca tgattcgggc
      661 cgttgtattc gatctcgata acacgcttgt cgatttcatg aagatgaaag atcgcgccgt
      721 agatgccgcg gcgtgggcaa tggtcgacgc cggtttgcag atgactcccg aggatgtgaa
      781 acgcggtatc tacgatcagt acgataaata cgggatcgaa tcgcaggacg ttttcgacaa
      841 gtatctgacg gaaatccttg gtgagatcga ctatcgcgtg cttgcggcgg gtatcatcgc
      901 gtaccgaaag gcgcgcgagg cttcgctgca accgtatcct cacgtacgca tcatcatcgc
      961 aaaactcctc cgccgaggcg tcaagctcgc cgtgctctcc gatgcgccgc gccgtgaagc
     1021 gtggctacga ctctgccaac tcggcctgca tcacgagttc gatgtagtcg tcacatacga
     1081 ggacaccgga tatcgaaagc cgcatccgga gccattcgcg cgcgtgatcg acatgctcgg
     1141 tatcgcaccg cgcgaagcgg tcatggttgg cgattggccg gagcgcgatg ttgccggtgc
     1201 gaaggttgcc ggactatgga ccgtgtgggc gaaatacggc agcgttcacg cggtagagac
     1261 gacaaacgct gactatgaga tcgagtcggt agacgaaatc gtggacgtaa tcgacgcgat
     1321 cgacgcgcgt atggcgtcta acacttcaga accgtgatcg cggtaaccgc acacaatcga
     1381 acgttcttcc attccgagaa cgctgtgacc tgctccgttt tcgcgcgaac tcccccgtct
     1441 tttacgaaac gcgcagagtt cacagaggag ttgacgtttc ccgaccgatc acagatcatt
     1501 acacatcggt atctgcttcc agctccggcg aataccaacg tcggtcaata tctcttccac
     1561 ctgtcgaata ggacgaatat gaacccgatc agcgagtttc tcgaagggaa aaccctgttc
     1621 ataaccggtg cgacggggtt ccttgcgaag gggctcgtag agaagctgct acgacaggct
     1681 cccggtatcc gccgaatcta cctcctgatt cggtcgtctc ggcaaaccga tggccggatc
     1741 gttaccgccg aacagcgact aaaacgcgat atcatcgact caagcgcatt ctctgcactt
     1801 cgcgcgctct ataaagacga ctatgaagat ttcgtggccg acaagctcgt cccagttacc
     1861 ggcgacctta catgcgagcg gctgggtatc ggcgtcgaga cgtatgacca gctctgccgc
     1921 gaagtcgata tcatcatcaa cagcgcggca acggtggtgt tcgacgagcg cgtcgatctg
     1981 gcgcttgata tgaacaccct cggtccgcgc cgagtactcg gtctcgcaca agactgtcac
     2041 gacgcagttt tcgctcacgt ctccacggcc tacgtcaatg gcgagcagat cggtgagctt
     2101 ccggaacggc ttctctcacc gacgcagtgc atcgcgaata tcacgagaaa cggcgcggga
     2161 ccggaatacg acctcgataa cgaaatcgcg cgtattcaag cgctccgtga tcaggtgctc
     2221 gcggaagcag gcagtaccga tctgacacag aaattcacga agatgctcga tcggcaggat
     2281 gccggcaagc gtgtgacgga gcaccgtcgt gaacaccagc tcgatgcatt acggcagcgc
     2341 tggatcacaa agcgtctggt aaacgaggga atcgcgcgcg cgcgtgagct cggttggcat
     2401 gacaactaca cgtacaccaa agccatgggc gagcagcttc tcgtgcgtga ccacggaaat
     2461 gtgccactgg tgatcgtccg tccgtcaatc atcgaaagca gcttgtatga accggaaccc
     2521 ggttggctcg acgggttgaa agtcgccgat ccgctcattg cacacttcgg taaaggtcgc
     2581 cttcccgatt tccccgggaa tccaagctca atcctcgatg tgattccagt cgacatggtc
     2641 gtaaacgtta tcatcggagc gttacccgaa gcccgtaacg gcaacgacgt gaaggtcttc
     2701 cacgtcgcaa caagcgcgga aaatccgctc accttcggaa gaatcgtcga tctgacctac
     2761 gattacttca aacggtaccc gatgcgtgac cgccacggag aaccgattcc cgtgcggcct
     2821 tggcacttcc caaggatcag cgccttccga cttcgatatt acctccggta ccgccttccg
     2881 gtaaccgtga tgtcgtgggc gattcagcat ctcaggttca tcaattggtc gtcacggctc
     2941 aagcggcgaa tcagcacact ggacgcgctt tccgaacgtg tgctatcgct ggtggatatc
     3001 tatagcccct acacgaacct gcactgtacg tacggcatcg caaacacacg cgcgcttttc
     3061 cagaatcttt cgccggaaga ccgtgagtcg ttgcgcttcg acgtttcgca gatcgattgg
     3121 cgggagtact tgcaggacat ccacctcccc gggttgaagc gccacgttct gaaaatgccg
     3181 ggtgaacgga agacctcgaa tgaacacacc gaggaagtat aatggcgagt cgcagtagca
     3241 aaatcccgta tcacgtcgag ctcctcggcg atcgcgcgac gatgtacgcc acgtacgacg
     3301 agtatgtcca acatttcctc gacgtcatca ggatggaagg ctacttcgac agcgatatgg
     3361 gcgagatcgt ctggccgacg ggttcggttg aacagggcac ggtcggtcgc gaagacctcg
     3421 tgcgacgctc ggcactcgtc aatgcagtct accgcggact ccccccgctg cgcgacgacc
     3481 gactggccga cgcgtggacc gagcttgagc gactcattcc gctgtacca
//
LOCUS       DSCC01000074            1815 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043971, whole genome shotgun sequence.
ACCESSION   DSCC01000074 DSCC01000000
VERSION     DSCC01000074.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043971"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..279)
                     /locus_tag="ENN56_00880"
     CDS             complement(<1..279)
                     /locus_tag="ENN56_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74070.1"
                     /translation="MADRLAIFKRRVSWSSPILFVASFFVMIVIGAVLLSLPFATVSR
                     GSMSLVDALFQSTSAVCITGLTTLTVATDLTLFGQIVILVLFQAGALGI"
     gene            complement(413..>1815)
                     /locus_tag="ENN56_00885"
     CDS             complement(413..>1815)
                     /locus_tag="ENN56_00885"
                     /inference="COORDINATES: protein motif:HMM:PF13520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS74071.1"
                     /translation="LFHLGVMAVFIALGLVYAFGHGFDFGLIISGTGTLTGLNGLTIA
                     IIIAGLGNAILGASGVESVMQIPEELDDPKSAVPKIYRWMLSILLLFGGLVTLMIFVV
                     LDPTELRTGQDFLVSMAGRKAMASITGSVLLGTIFETIIVIDAALTLIGATNTSFAGI
                     RGLWVTMAKDHLMPRFFLRTDRVLGTHRNIHIMFLLGIALLAIGAEADIEVLGHWYGG
                     TFGLVLFSGVIGIVLLRYFLPNAARPYRAPFNVKIAGFEIPFGAIIGFVFVGGALISL
                     HLSARPDVEQLGVLLTYLLVLVIIVIAYYGHPRWMRAWNRYREEILLEHRGELRLNRE
                     RAVAVCVGGFRARERILFMLEYAAMQQYSHLVVLHVTDSTDHVSVFDPYDSATRYLEI
                     PGLNARRILETLATVPNIEEVQTHFVVIPLTNHRTWQEAVNTFLSEVPEIHFVATGPG
                     EELDARLAKVLVINTE"
BASE COUNT          485 a          465 c          490 g          375 t
ORIGIN      
        1 aattccgagt gcaccggctt gaaacaacac aagaatgacg atctgaccga ataatgtcag
       61 atcggttgct acggtcaacg ttgtaaggcc ggtaatacat accgcgctcg tactctggaa
      121 taacgcatcg acaagcgaca tcgacccgcg cgatacggta gcgaatggga gggacaagag
      181 gacggcaccg attacgatca tcacgaaaaa actcgcgacg aaaaggatcg gcgacgacca
      241 agaaacacga cgtttgaaaa tcgcgagacg atctgccatg agaattcgag ttctcgattg
      301 gttgtgtgcc gacgaataaa ccgaagtcgc gataccgttg atacttctgc atcaaggtat
      361 gccgagaacc gaggctgtgc aagtcatcgg aaaggcgtat ggcttaccga agtcattccg
      421 tattgataac aagcactttc gcgagccgag catcgagttc ctcacccgga ccggtggcga
      481 cgaaatgaat ctcggggacc tcagagagaa atgtgttaac cgcctcttgc caggttctgt
      541 gattagtgag cggaataaca acgaagtgtg tctggacttc ctcgatatta ggaaccgtcg
      601 cgagcgtttc gaggatacgt cgagcattta gaccggggat ttcgagatac cgagtcgccg
      661 aatcgtacgg gtcaaaaacg gagacgtgat ccgtcgagtc cgttacatgt agcacgacaa
      721 gatgggaata ctgttgcatc gcagcgtatt cgagcatgaa caggatacgt tctctcgcac
      781 ggaatccacc tacgcagaca gcaacggctc gctctctgtt gagtctcaat tcaccgcgat
      841 gttcgagcag gatttcttca cggtatcgat tccatgcccg catccaacgt gggtgtccgt
      901 aatacgcgat aacgataatg acgagaacaa ggagataggt gagcaataca ccgagttgtt
      961 ccacgtccgg tctggctgag aggtgtagac taataagagc tccacctacg aaaacgaaac
     1021 ctattatcgc accgaacgga atctcaaagc cggctatttt cacgttaaac ggcgcgcggt
     1081 acggtcttgc tgcgttcggg aggaaatatc ggagcaacac gattccaatc acaccggaaa
     1141 acaatacgag cccgaatgtc ccaccgtacc aatgaccaag cacctcgata tccgcttccg
     1201 caccgattgc gaggagcgcg attccgagga gaaacatgat gtgaatgttg cggtgtgtac
     1261 cgagtacacg gtccgtacga aggaaaaagc gtggcatcaa gtggtctttc gccatggtta
     1321 cccacaatcc gcgaataccg gcaaaggacg tgttcgttgc accgatcaac gtgagtgcgg
     1381 cgtcgatgac gatgatcgtc tcgaagatcg ttcccaagag tacggacccg gtaatgctcg
     1441 ccatggcttt tcgtcccgcc atggagacaa ggaaatcttg gccggttcgc agttccgtcg
     1501 gatcgagtac cacgaatatc atgagtgtga ccaaaccgcc gaacaggagc aggatactga
     1561 gcatccaccg atagattttc ggaacggctg actttggatc gtcgagttcc tcgggtatct
     1621 gcatgacgct ttccactccc gatgctccga ggatggcgtt tccgagtccc gcgatgataa
     1681 tcgcgatggt caggccgttc agaccggtaa gtgtgccggt tcccgatatg atcagcccga
     1741 aatcgaaacc gtgcccgaac gcgtatacca gccctaacgc gatgaagact gccattacac
     1801 cgaggtggaa tagga
//
LOCUS       DSCC01000075            3156 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020868, whole genome shotgun sequence.
ACCESSION   DSCC01000075 DSCC01000000
VERSION     DSCC01000075.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020868"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..496)
                     /locus_tag="ENN56_00890"
     CDS             complement(<1..496)
                     /locus_tag="ENN56_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HDS74072.1"
                     /translation="MSSQSLYPKAFERPRFMNSADARGIRILAEYYEPLQRLNNEQIW
                     HTIVFFGSARFIDREKADAALQAAREANASAEVIRAAEWKLEMARYYEDARKLAYLLT
                     EWSLALPDDVRMWICSGGGGGIMEAANRGAAEAGGKTVSLNIHLPHEQKPNDWAQESL
                     TFNFH"
     gene            complement(575..1402)
                     /locus_tag="ENN56_00895"
     CDS             complement(575..1402)
                     /locus_tag="ENN56_00895"
                     /inference="COORDINATES: protein motif:HMM:PF10609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDS74073.1"
                     /translation="MALIIAVVNPKGGVGKTTTAINIASSLAIIQRPTLLIDMDPDGA
                     CAIGLGFDPSAIHGGVFDVFTDHTSLSTTIHQTSLPYLHFIPTNVVTSDHESQFAEVA
                     ADRRLMRDLLRGPVADSYEFVILDCPPSLANVTINALVAADTVLIPVNASQFAVKAII
                     RLIKLIRILRDKQANEKLTVAGLLVTMHDKRTRVGLQIEKDLRDMFKHLVFDTTIPIN
                     TRVNEANYKGRPVVTYDVNGAGSVAYMDATLELLSRVGKTPRRRSDVALGHRNVAHD"
     gene            complement(1433..2062)
                     /locus_tag="ENN56_00900"
     CDS             complement(1433..2062)
                     /locus_tag="ENN56_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74074.1"
                     /translation="MKGLLLEGIVAAGRSEIIRALRDHAAWRRRESTLILNDLFTERA
                     NEHLRSLTPESYRTLMLRNLRILQAAKQIESASPLFDPDDRRDLCYVLDGFHITNAIV
                     HADGEFEAFADIDVELERLDCRLVLVMINEKVVGSRIKDVYRRRDERWRAYQERLESR
                     VGDLSAYYVTLQHTYLDTLNKTALKNITIDTTDQDWRRCAEDIMRFWRI"
     gene            complement(2160..3149)
                     /locus_tag="ENN56_00905"
     CDS             complement(2160..3149)
                     /locus_tag="ENN56_00905"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08534.8,HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HDS74075.1"
                     /translation="MIHMQGIFAFVIMATLVGAEPMLSSEESLRRITEVIERPVRNSE
                     MKARDYQQALTMLDRFARDYPDAVSLATADFLRANAYLGLGQTEYAQSSLEAALAGPL
                     PNDHLVVANYLHGRTLLENGQNKDGVAALRRAIGSNPNHPAVAEVRFILAYALMENLD
                     AIEAQGQLDTLMATGGPQWVLDGAATLRKNLRWIGQAAPTFNARTLDGRQISLRDFEG
                     RVVLIDFWASWCPPCIAMLPDLQNVYAKYRTRGFEVIGMSLDRTRSDLFGFLRSNDMP
                     WIHVHENAEIVADRYGVEGIPMTFLLDRRGTIAAVNLHGERLAAAVERLLSAR"
BASE COUNT          720 a          891 c          811 g          734 t
ORIGIN      
        1 agtggaaatt gaacgttagc gactcttgcg cccaatcatt cggtttttgc tcgtgcggca
       61 gatgaatatt caatgatacg gtcttcccgc cggcttcggc agctcctcga ttggcggctt
      121 ccataatccc tccgccgcca ccggaacaga tccacatgcg cacgtcgtcc ggtaacgcca
      181 acgaccattc cgtgagtaag tacgcgagct tccgcgcgtc ctcgtaatac cgtgccatct
      241 caagcttcca ctccgcggca cgtatcactt ccgccgaagc gttcgcctca cgtgcggctt
      301 gtagtgcggc atccgctttt tcccgatcga taaaccgagc agaaccgaag aagacaatgg
      361 tatgccatat ctgttcattg ttgagtctct gcaatggttc gtagtactcg gcaagtattc
      421 gtatgccacg ggcatccgca ctattcatga atcgcggccg ttcaaatgct ttcgggtaga
      481 gagactgtga ggacatgggg acctttcgtg tatactcgat cctgaggata gagagtgact
      541 gagatttgtg agttagaaac ggggttttca atcgctaatc gtgcgccaca tttctgtggc
      601 ccagcgcaac gtcgctacgt cgacgcggtg ttttcccaac gcgactgagg agttcgagtg
      661 tcgcatccat atacgcaacg gaacccgcgc catttacatc gtacgtaaca accgggcgac
      721 ctttgtagtt cgcttcattg acacgggtat tgatcggaat cgtcgtatcg aacacaagat
      781 gcttgaacat atcacggagg tctttctcga tctgaagccc tacccgcgtt cgtttgtcgt
      841 gcatcgtaac gagtaagcct gcaacagtaa gcttctcatt cgcctgtttg tcccgaagta
      901 tccgaatgag tttgatgagg cgaatgatag cctttacggc aaactggctc gcgttgacgg
      961 gaatcagcac cgtatcagcg gcaacgagtg cattaatcgt aacgttggcg agcgaaggcg
     1021 ggcaatcgag gatgacgaac tcgtacgagt cggcaaccgg gcctcgtaag aggtctcgca
     1081 taagccgccg atctgcagca acttcagcga actgcgattc gtgatcactc gtgacgacgt
     1141 ttgtcggaat aaaatgaagg tacggtaaag acgtctggtg tatcgtcgtt gagagcgacg
     1201 tgtgatccgt aaagacatcg aaaacgccac cgtggatcgc tgacggatca aatcccagac
     1261 cgatcgcgca cgcgccgtct ggatccatat cgataaggag cgtcgggcgt tgaataatcg
     1321 caagtgatga agcgatattg atcgcggtcg tcgttttccc cacaccgcct tttggattca
     1381 caacggcaat aataagcgcc attccgtccc tctcttgccc gatatttcgc tgtcagatac
     1441 gccaaaatcg catgatatct tcggcgcaac gtcgccaatc ctgatctgtc gtatcgatcg
     1501 ttatgttttt cagtgcagtc ttgtttaacg tgtccagata cgtgtgttgc agtgtcacgt
     1561 aatacgcgga caagtcaccc actcgcgatt ccaagcgttc ttgatatgcc cgccaccgtt
     1621 catcgcggcg acggtacacg tctttgatcc gtgaaccgac aaccttttcg ttgatcatga
     1681 cgagcaccaa tcgacaatcg agccgctcaa gttccacatc gatatctgcg aacgcttcga
     1741 actcaccgtc cgcgtgcact atcgcgtttg taatgtgaaa cccatcgagt acataacaca
     1801 gatcacgccg atcgtcggga tcgaaaagcg gactcgcgct ctcaatctgc ttagccgctt
     1861 gaaggatccg cagatttcgc aacatgagcg tgcggtaact ctccggagtc aacgatcgaa
     1921 gatgctcgtt cgcacgttca gtaaagagat cgttcaagat gagcgtggat tcgcgacgac
     1981 gccacgcggc atgatcacgg agcgcacgga taatctccga tctccctgcc gcaacgatcc
     2041 cttccagcaa tagacctttc atggcatacc accgccggtt aggtcattca gccgtcaatc
     2101 gcaacaattc tttcgttgta agataccaca ccgatgacca tggccaaggg atagagcggt
     2161 tatcgcgcgc tgagaagccg ctcgaccgcc gctgccagtc gttctccgtg gagattcacg
     2221 gcggcaatag tcccacgacg gtcgagtaag aacgtcatcg ggattccctc gacaccgtac
     2281 cgatccgcga caatctctgc gttttcgtgt acgtgtatcc acggcatgtc gttcgaacga
     2341 agaaacccaa aaaggtcgct acgtgttcga tcgagactca tgccaatgac ttcgaacccg
     2401 cgcgttcggt acttcgcgta tacgttttgc agatccggta gcattgcgat acacggagga
     2461 caccaactcg cccagaagtc gatgagcacg acacgccctt cgaaatctct aagcgagatc
     2521 tggcggccat cgagagtccg agcgttgaat gttggtgcag cctgtccgat ccaccgcaga
     2581 ttcttacgta gagtcgctgc cccatcgaga acccattgcg ggccgcccgt tgccatcaag
     2641 gtatccaact gcccttgcgc ctctatcgca tcgaggtttt ccatgagagc gtacgcgaga
     2701 ataaaccgta cctctgcaac ggccgggtga ttcggattcg aaccgatagc gcgacgtaac
     2761 gctgcgacgc cgtctttgtt ttgcccgttt tcgagcaggg tgcgtccgtg aaggtaattc
     2821 gcgacgacga gatgatcgtt cggaagtggt ccagccaacg cggcttcaag cgacgattgc
     2881 gcgtactcgg tctgcccaag tccaagatac gcgttcgcgc gaaggaaatc ggcggtcgcc
     2941 agactcaccg catcgggata gtcgcgcgcg aatcggtcca gcatcgtgag ggcctgctgg
     3001 taatcgcgtg ccttcatttc actattccgt accggacgct caatcacctc agttatcctt
     3061 cgaagcgatt cctccgagct gagcatcggt tcggcaccaa ccaatgttgc cattatgacg
     3121 aacgcgaaga ttccctgcat atgtatcatt tacgac
//
LOCUS       DSCC01000076            1553 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054254, whole genome shotgun sequence.
ACCESSION   DSCC01000076 DSCC01000000
VERSION     DSCC01000076.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054254"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..779
                     /locus_tag="ENN56_00910"
     CDS             <1..779
                     /locus_tag="ENN56_00910"
                     /inference="COORDINATES: protein motif:HMM:PF11175.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDS74076.1"
                     /translation="TEEYFNWGWYDVLPKDRVFTFPVHGYTEHSRDVDDHTTMYRFHL
                     TDSVPFYRSLRFDFEHGPIGDTPANYESVAFTHHRDEPVLVLSDVIAIDNVSRTENVS
                     GAMPRGHRTLVYEGNDQVIEQKRKAGDVPESWYDVGGITDTGRVFTGSATYTVAIHGG
                     NQGIKVRRRSDGEFPSGECPENERSRVVPEQRVRVAIDGAEVGVWLMPAHHARRTWLE
                     DDFEIPARFTAGKDRITIQLKNESDIGWNEYRYWIYSYTE"
BASE COUNT          346 a          468 c          418 g          321 t
ORIGIN      
        1 gaacggagga gtatttcaac tggggatggt acgacgttct cccgaaagac cgcgtattca
       61 cgtttcccgt gcacggctac accgaacaca gccgcgacgt tgacgatcac acgacgatgt
      121 accgctttca tctcacggac tcggttccat tctaccggag tcttcgattc gattttgaac
      181 acggcccgat cggcgatacg cctgccaact acgaaagcgt cgcgtttacg caccatcgcg
      241 acgaaccggt cctcgtgctg agtgacgtga tcgcgatcga caacgtatcg cgcacggaga
      301 atgtcagcgg cgcgatgccg cgagggcacc gaacactcgt gtatgaggga aacgaccagg
      361 ttatcgaaca gaagcgcaaa gcgggtgatg ttcccgaatc atggtatgac gttggcggga
      421 ttaccgatac cggacgtgtg tttaccggtt cggcgacgta caccgtcgcg atccacggag
      481 gcaaccaagg aatcaaggtg cgtcgtcgct cagatggcga gttcccttcg ggagagtgcc
      541 ccgaaaatga acgttcgcgc gtcgtgcccg agcaacgcgt ccgtgtagcc atcgatggcg
      601 cggaggtcgg cgtgtggctg atgccggccc atcacgcccg aagaacatgg ctcgaagatg
      661 atttcgagat acccgcgcgg ttcactgccg gcaaggaccg catcacgatt cagttgaaga
      721 acgaatcgga cattgggtgg aacgaatatc gatactggat ctactcgtat accgagtaac
      781 agtgcgattc acacgcccac attcaatccg atttgagcgt gctcgccgat ctcgttctcc
      841 acacgccacg gaccttcgca tacgttaccc gttacgaccg ccgacgtgac tccgctgcga
      901 atcgcgacct gcggcgcggt catctcccgg ttcatcgact tgtcgaagtg atttccgacg
      961 atagataccg taccgcccga aacatcaatc gcggcgacat tccgtttgtt tttgtcccac
     1021 tgaatgaagt tgcagccgtt gatcgacacg cgacccttcc ccgcgatacg cgcgatgcta
     1081 tcggacggtc cccagacacc gcagttcgtc agcgaaatga agcccgagtt cgtctccttc
     1141 accacgagcg cggctgagtt ttccgccatg agcggcacga actccccgtt cgtaacaagc
     1201 acaccgccgg tgtggtcgtt gatttggtcg acaacgacgc attcttccgc cgcatcgact
     1261 ccaacaccga tgaactgccc gttcgccgag ccatgttccg tctggatgaa gtggatgccg
     1321 actcgatatc cccaacagaa cgtgttatac acgtattccc agtccgtccg tccgaacacg
     1381 aacgccgtac tgttgtgcgg caaccaattc atgtagaagt cttcgtgtcg ttctggtcca
     1441 ttgaacggcc aatagtgcac gttctcaatg cgaccgacgt cgtagcattg atcgacatat
     1501 aagcccgtat gaagcggact cccgtgcaca tcgcgaacaa gatgccgaca cgc
//
LOCUS       DSCC01000077            2479 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028782, whole genome shotgun sequence.
ACCESSION   DSCC01000077 DSCC01000000
VERSION     DSCC01000077.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028782"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            55..237
                     /locus_tag="ENN56_00915"
     CDS             55..237
                     /locus_tag="ENN56_00915"
                     /inference="COORDINATES: protein motif:HMM:PF10406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74077.1"
                     /translation="MTTQQRRLYGMPELPRYRSYLTTPRSTSKRKRDSRSIRRRSCST
                     KRRISTKRGTRNTPSK"
     gene            415..>2479
                     /gene="infB"
                     /locus_tag="ENN56_00920"
     CDS             415..>2479
                     /gene="infB"
                     /locus_tag="ENN56_00920"
                     /inference="COORDINATES: protein motif:HMM:PF04760.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDS74078.1"
                     /translation="MARHTRVYQVAKEHRISSEVLMGILPDLGIEVKNHLDKLEDEEL
                     DRVAEWFAKYPDGEAPSDTGESAAEEAPTAEPSAASTDAETPEADIKPEKTEEKKQAE
                     PAPHPVMPPPEVVTTQVFMPPVETTPPPPPPPKPAARQQPKQVEKKPAVEEAAKGDEL
                     RQTPKRTETQVERTSKPEKTEKTDKPDKAKRSKRDERPERAPERRREEGTRDPEVEEI
                     SGRKIVRGHATTDRVDPQGAAGAGRRRRTKRKKKPVASQEEIQKNVQKTLTEMGRGRT
                     RRTYDRRGSHEEEEIQEERNVVRVTEFVSVSEFADLMDVKANEVIAACLKLGLMVSLN
                     QRLDWDTILVIADEFDFEVEQMEEKEVEEVVEEEDRPEDLLPRHPVVTVMGHVDHGKT
                     SLLDYIRKTNVIGGESGGITQHIGAYEVETANGRVTFLDTPGHEAFTAMRARGAQVTD
                     VVVLVIAADDAVMPQTVEAINHARAANVPLFVAINKVDLPAANPAKIKQQLTEYNVVV
                     EEFGGKSQAVEISAKTGQGINELLEMLALETELLDLKANPNKPARGVVVEARLDKGRG
                     PVATVLVESGTLRIGDPFLAGMHAGRVRAMLDERGNRIEEAGPSKPVQVLGLGDVPTA
                     GDSLLVYHDEREARDIAQYRQQLKREQDYNRTKKISLRNISERISDGELKTLPVIVKG
                     DVDGSV"
BASE COUNT          649 a          703 c          746 g          381 t
ORIGIN      
        1 agcgacagga gcggaaagag ttcctggcgg tgccgatcga cgagcttcca gacattgacg
       61 acgcaacaaa gacggctcta cggaatgccg gaattaccac ggtacaggag ttacttaact
      121 actccgcgtt cgacctcgaa gagaaaacgg gactcccgct cgatacgacg gcgcagttgc
      181 tcgacgaagc gcaggatctc gacgaagcgc ggcacgagga atacgccaag caaatgagca
      241 taccggtaga atacaccggc gtcgatctcg cgacattcgt tgcggatccg ccgccattgg
      301 tcgaagagtc cgtcgcggaa gacgaagagc aagaagcggt ttcggcggag taatcgaatc
      361 acggaaagcc ggacgggcag gtattgcgcc tgtttgccgg gaggtatttt cagggtggca
      421 aggcacacac gagtctatca ggtagccaaa gaacaccgga tcagctccga agtcctcatg
      481 ggtattctgc ccgatctggg catcgaagtg aagaaccatc tggataaact cgaagacgaa
      541 gagcttgacc gcgtcgccga gtggttcgcg aagtaccccg atggtgaagc tccttcggat
      601 acgggcgaaa gcgccgcaga agaagcgccg accgccgaac cgtctgcggc atcaaccgac
      661 gctgaaactc cagaagcgga cataaaaccg gagaagacgg aagagaaaaa acaggccgaa
      721 ccagcgccgc atccggtcat gccgcctccc gaagtggtca ccacgcaggt gttcatgcca
      781 ccggtggaga caactcctcc gcctcctccc ccaccgaagc ccgcggctcg gcagcagccc
      841 aaacaggtgg agaagaaacc ggccgtcgag gaagctgcga aaggcgacga actccgtcaa
      901 acgccgaaac gcacggaaac tcaggttgag cggacaagca agccggaaaa aacagagaag
      961 actgataaac ccgataaagc gaagcggtcc aagcgtgacg aacgacccga gcgcgctccg
     1021 gaacgtcgac gcgaagaagg cacgcgcgac cccgaagtcg aggaaatctc cggtcgaaaa
     1081 atcgtccgag gccacgcaac gacagaccgt gtcgacccgc aaggcgcagc gggcgccgga
     1141 cggcggcgcc ggaccaagcg gaagaaaaaa ccggtcgctt cgcaggaaga gatccagaag
     1201 aacgtccaga agacacttac cgagatggga cgcggccgca ctcgacgtac ctacgatcga
     1261 cgcggctccc acgaggaaga agagattcaa gaagaacgca atgtcgttcg cgtcaccgaa
     1321 ttcgtatcgg tgagcgagtt tgctgacctc atggacgtaa aggccaatga ggtgatcgcg
     1381 gcgtgcctga agctcggtct gatggtctcc ctcaatcaac gtctcgactg ggatacgatt
     1441 ctcgttatcg cggatgaatt cgacttcgaa gttgagcaga tggaagagaa ggaagtcgaa
     1501 gaggtcgtcg aggaagagga tcgtccggaa gacctgctac cacggcatcc ggttgtgacc
     1561 gtcatgggtc acgtcgacca cggaaagaca tcgcttctcg attacatacg gaagacgaac
     1621 gtcatcggcg gcgaatccgg cggcatcacg cagcacatcg gtgcgtacga agttgaaacg
     1681 gcaaacggcc gcgttacatt cctcgataca ccggggcacg aagcgttcac cgcaatgcgt
     1741 gcgcgaggcg ctcaagtcac cgacgtcgtc gtactcgtaa tcgccgcgga cgacgcggtc
     1801 atgccgcaga ccgtcgaagc gatcaaccac gcacgagcgg cgaatgtccc gcttttcgtt
     1861 gcaatcaaca aggtcgatct tccggcggca aacccggcaa agatcaagca gcaactcact
     1921 gaatacaatg tcgttgtgga agaattcggt ggaaaatcgc aggctgtcga gatttcagcg
     1981 aagaccggtc agggaatcaa cgagctgctc gagatgctcg cacttgaaac ggaattgctc
     2041 gacctgaagg cgaatccgaa caaaccggca cgcggtgtcg ttgtagaagc tcgcctcgat
     2101 aagggccgcg gccccgttgc gacggtactc gtcgaatcgg gcacgttgcg tatcggcgat
     2161 ccgtttcttg ccggaatgca cgccggtcgc gttcgcgcga tgctcgatga acgcggaaat
     2221 cgaatcgagg aagcaggtcc ttcgaagccg gtgcaggtac tcggacttgg tgacgtccca
     2281 accgccggtg actcgttgct cgtctaccac gatgaacgtg aagcgcgcga cattgcgcag
     2341 tatcgccagc agctcaagcg cgaacaagac tacaatcgca cgaagaagat atcgctgcgc
     2401 aacatcagcg aacgcatcag cgacggcgaa ctcaagacgc tgccggtcat tgtcaaaggc
     2461 gacgtggacg gctccgtcg
//
LOCUS       DSCC01000078            1553 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054250, whole genome shotgun sequence.
ACCESSION   DSCC01000078 DSCC01000000
VERSION     DSCC01000078.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054250"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(210..1139)
                     /locus_tag="ENN56_00925"
     CDS             complement(210..1139)
                     /locus_tag="ENN56_00925"
                     /inference="COORDINATES: protein motif:HMM:PF00459.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74079.1"
                     /translation="MISFGRNPAQISAYRILQTNLSLDLRPVRCTFYQIPFHRRETGE
                     RMDEVTGIIEEISSIARQAGDIALRSFGRVSPEMKADRSFVTEADRRVEEFIREQLAS
                     RFPADNVIGEEDIDKSDGSSGYTWAIDPIDGTTNYVFGVPYWAVCIGRIAADGTPDLG
                     VVNVPAMDELYTGALGNGATVNGRTLKMAVPAFPEHELLLGAWSTFYREIELDFQGKV
                     RVLGSTVIKFLNMARGMFIGALTPTVHIWDVAPGFPILREAGGDICLRDGTPYERFDL
                     DPKHEFLVPPLVLAASSEMDRIRGMIVEQRKHE"
     gene            1206..1278
                     /locus_tag="ENN56_00930"
     tRNA            1206..1278
                     /locus_tag="ENN56_00930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:1239..1241,aa:Lys,seq:ctt)
BASE COUNT          331 a          440 c          429 g          353 t
ORIGIN      
        1 acgtacgagt tgaggtcgac gacgtttcgg ttccgccttg gcgaaggcgg gctttctcgg
       61 cttccgcgta cgcgcgcttt gcgttgaaaa acgtgttgta gtacacgcaa ccgccgaatg
      121 acagcacgat caccgcgcac agagacatga atcgtaacag agatctcata gcttcatctg
      181 atccggatgc cttccactgg taccggtaat cattcatgtt ttcgctgctc gacgatcatt
      241 ccgcgaatcc tatccatttc gcttgacgcc gcgagcacca gtggcggaac gagaaactcg
      301 tgtttcgggt cgagatcaaa ccgttcatac ggcgtaccgt cgcggaggca gatatcgcct
      361 cccgcctcgc ggaggatcgg gaatcctggc gcgacatccc agatatgcac tgtaggtgtg
      421 agtgcgccaa tgaacatgcc gcgcgccatg ttaaggaact tgataacggt gctcccaagc
      481 acccgtacct tcccctgaaa atcgagctcg atctcccggt aaaacgtcga ccacgcgccg
      541 aggagtaact cgtgttcggg aaaagcggga acggccatct tcagcgtacg accgttcaca
      601 gtcgcaccgt ttccgagtgc gccggtatag agctcgtcca ttgcgggtac gttcacaaca
      661 ccgagatcag gtgtgccgtc ggcggcgatg cgcccgatac agaccgccca gtagggaaca
      721 ccaaacacgt agtttgtcgt gccgtcaatc gggtcgattg cccaggtata gcctgatgaa
      781 ccatcggatt tatcgatgtc ttcttcaccg atcacgttgt ccgctgggaa tcgcgacgcg
      841 agttgctcgc ggataaactc ttcaacgcgt cggtcggctt ccgtcacgaa cgaacgatcg
      901 gccttcatct ccggactgac tcgtccgaac gagcggagag cgatatcgcc ggcctgccgc
      961 gctatagaac ttatctcttc aatgatacct gttacttcgt ccatgcgttc tcccgtttcc
     1021 cttcgatgaa atggtatttg gtagaacgta caccgcactg ggcggaggtc aagcgaaagg
     1081 ttcgtctgta ggatgcgata cgcggagatt tgcgcggggt tccggccgaa ggaaatcatc
     1141 gcgaacggcg ttgccttgac tgaaagattg gcgatagata ttttataaag tctgacgagt
     1201 tcaatgcctc cgtagctcag ctggtagagc agctgactct taatcagttg gtcgtaggtt
     1261 cgaatcctac cgggggtact tccgaaaacg gcacgttcga gaccactcga gggtgccgtt
     1321 ttttcatgct gcggcgtcgt cgcgacacgc tcgacgacat gaaagcgtac gtgtcgagac
     1381 gttgcacgtg tcccttcccg acacccggcg aagacgcgtt cgcgtcttcg ccttacgaac
     1441 tcaagcttgc ttgagttcgc aggccaaacg gtcccttgtc atccgactcg atcgcggcga
     1501 ggaactcgtc gatcttatcg gcggtaacgt agcggtagag cttcttattc acg
//
LOCUS       DSCC01000079            1933 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040319, whole genome shotgun sequence.
ACCESSION   DSCC01000079 DSCC01000000
VERSION     DSCC01000079.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040319"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1298)
                     /gene="nusA"
                     /locus_tag="ENN56_00935"
     CDS             complement(<1..1298)
                     /gene="nusA"
                     /locus_tag="ENN56_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HDS74080.1"
                     /translation="MNNEEVLEALRQIAREKNVTLDLVIDTLEEGLLSAARKKYGTAE
                     NIDVEVDQNTGEIIIWAHKAVVDEVEEPALEVTLKDAKKFDSEAEVGEAVMQPIPFTV
                     FGRMAIQAAKQMIVQKVREAERERVYETFREQVGEIRIGSAQQVLRGDVIVNLGRAEG
                     VLPTTEQIPRERFRQGDTIRAYVKDVLRETKGPQVILSRTHPEFLEQLFHIEVPEIYD
                     GLVEIMAVARDPGNRAKIAVTSHDERIDPVGACIGPKGSRVQAVVRELNNERIDIIEW
                     SSDPETFLLNSLAPAEVQRLIVEHETRTMIAIASEDQLSPAIGRGGQNVRLAAMLTGW
                     RVEVLSEEQHQQRQERKEFLAVPIDELPDIDDATRTALRDAGITTVQELLNYSAFDLE
                     EKTGLPLDTTAQLLDEAQDLDEARHEEYAKQMSIPVEYTGV"
     gene            complement(1295..1840)
                     /locus_tag="ENN56_00940"
     CDS             complement(1295..1840)
                     /locus_tag="ENN56_00940"
                     /inference="COORDINATES: protein motif:HMM:PF02576.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74081.1"
                     /translation="MSVKLTVLPHEDLVSTDTHAAEVVAEQVVVPLGVELLEAKWIGP
                     RTQRTLRLVVDSEGGVSADICAAVTQQFDLVWEAEEATPRDFGLEVTSPGPNRPLVTE
                     RDFTRVTGKWVEVVYRDGETDRVTVLAQVDGAAEGVLRMTGLDDVETIPIGDIERAKV
                     VFTIGKPPTPKRTLERRQKSS"
BASE COUNT          353 a          616 c          506 g          458 t
ORIGIN      
        1 acgccggtgt attctaccgg tatgctcatt tgcttggcgt attcctcgtg ccgcgcttcg
       61 tcgagatcct gcgcttcgtc gagcaactgt gccgtcgtat cgagcgggag tcccgttttc
      121 tcttcgaggt cgaacgcgga gtagttgagt aactcctgta ctgtcgtaat tccggcatcc
      181 cgaagagccg tcctcgtcgc gtcgtcaatg tctggaagct cgtcgatcgg caccgccagg
      241 aactctttcc gctcctgtcg ctgctgatgc tgctcttcgc tgagcacttc aacgcgccag
      301 ccggtcaaca tcgctgcgag ccgaacgttt tgcccgccgc gtccgatggc cggcgaaagt
      361 tgatcttcac ttgcgatcgc gatcatcgtt cgtgtttcgt gctcgacgat gagacgctgg
      421 acttcagccg gtgcaagtga gttcagcaga aacgtttcgg gatcggaaga ccattcaatg
      481 atgtcaattc gctcgttatt gagctcgcgt acgacagctt gcactcggct tcccttcggt
      541 ccgatacacg caccgaccgg gtcaatccgc tcgtcgtgcg acgttacggc gatcttcgct
      601 cggtttcccg gatcgcgtgc aaccgccatg atttcgacga gcccgtcgta tatctccggc
      661 acttcgatat gaaaaagctg ctcgagaaac tccggatgcg tcctcgaaag gattacctgc
      721 ggtcccttcg tctcgcggag tacgtctttc acgtaggcgc gaattgtatc gccctgacga
      781 aagcgttcgc gaggaatctg ttcggttgtc ggtagaacgc cttcagcgcg cccgaggttc
      841 acgataacat cgcctcgaag cacctgctga gcggagccga tacgaatctc gccgacctgc
      901 tcgcggaatg tctcgtagac gcgttcacgt tcggcttcgc ggaccttttg gacgatcatc
      961 tgcttggcgg cctgaatcgc catgcgaccg aataccgtga acggtatggg ctgcatgacc
     1021 gcttcaccaa cttcggcttc ggaatcgaat ttcttcgcgt ccttcagcgt cacttcaagc
     1081 gccggctctt cgacctcgtc gacgacggcc ttgtgcgccc agatgataat ctcgcccgta
     1141 ttctgatcga cctcgacgtc gatgttctcg gcagttccgt atttcttccg tgcggccgag
     1201 agcagccctt cctcgagcgt atcgatgacg aggtccagcg tgacgttctt ttcgcgggct
     1261 atctgccgca gcgcctcaag tacctcttca ttgttcatga gctcttctgt ctccgttcca
     1321 acgtgcgctt cggagttggc ggttttccga ttgtgaatac caccttcgcc cgctcaatat
     1381 caccaattgg tatagtctcc acatcgtcaa ggcctgtcat gcggaggact ccctccgccg
     1441 cgccgtctac ttgcgcgagg accgttacac gatcagtttc accgtcgcgg tagacaacct
     1501 cgacccactt gcccgttact cgggtgaaat cgcgctccgt caccaagggt cgattcggcc
     1561 cgggcgaggt aacttcgagg ccgaaatccc gcggggttgc ctcttcggcc tcccacacga
     1621 gatcgaactg ttgcgtcacc gccgcacaga tatcggcgct gacaccgccc tccgaatcga
     1681 ccaccaatcg cagcgtgcgc tgcgtacgcg ggccgatcca cttcgcttcg agcaactcga
     1741 cacccagtgg gaccacgact tgctccgcca caacctcggc ggcatgcgta tccgtgctca
     1801 cgaggtcctc atggggtaaa acagtgagct tcacactcac ttgtaatcaa tggaaaatac
     1861 gaatatctac tcgcgaaagc aattgttgcc cctcggcccg aacacccgag ccgattcacc
     1921 aaccgctcgt cat
//
LOCUS       DSCC01000080            1553 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054259, whole genome shotgun sequence.
ACCESSION   DSCC01000080 DSCC01000000
VERSION     DSCC01000080.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054259"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            142..1131
                     /locus_tag="ENN56_00945"
     CDS             142..1131
                     /locus_tag="ENN56_00945"
                     /inference="COORDINATES: protein motif:HMM:PF01916.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74082.1"
                     /translation="MSENAEHRHVKDPVRAIRPSEHSDADGLMAAFSHMSFQARNLGQ
                     AADLLTTMAREEGLFKVLTLAGALIPAGMSETVREMIEHRMIDVIVSTGANMSHDLVE
                     GFGFHHYRNTDLKNDLELREAYINRIYDTYLAEDAFAGTEEKLLPHLKNYDGVTSADL
                     TRYLGTVVERPCVLKTATEHDVPIFIPALNDSELGIAVNRYNHLNRGGHQVRWDGLAD
                     NEAFANMMKHTDTQGIVICGGGVPRNWAQQVTPLLEYLNLGAGEEFERSLPGYKYGIQ
                     ITTDVPDYGGMSGCTFSESISWGKYDVNARYVRVSCDTTIALPLIVAAAIARL"
     gene            1156..>1553
                     /locus_tag="ENN56_00950"
     CDS             1156..>1553
                     /locus_tag="ENN56_00950"
                     /inference="COORDINATES: protein motif:HMM:PF00781.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74083.1"
                     /translation="MGVSWVVIANPVAGKGRRMRGLSVAVDALKQQGIDTQVVWTRCP
                     GDGRERAHEVAEQGHTNIAVAGGDGTANEVLNGLADTGRLSEFTITTLPFGTGNSFLR
                     DFDMNDPRVAIDRVIRGDARPCDLLRCTIQR"
BASE COUNT          354 a          426 c          461 g          312 t
ORIGIN      
        1 attcataatg gttcacagat atggccgtcg caatcgtctc aaggagattc tcaccgagac
       61 gattgcgacg gtaatgttgt atcgatcact cggaacaaac gaccgaaaga agtcgtcacg
      121 catctgattg gagattggaa catgagtgag aacgcagaac atcggcatgt gaaagaccca
      181 gttcgcgcca tccgtcccag cgaacactcc gatgccgacg gactgatggc tgcattttct
      241 catatgagct ttcaagcgcg aaacctcggt caagcggccg atctcctcac gacgatggcg
      301 cgtgaagaag gtctgttcaa ggtcctcacg ctcgccggtg cgctcattcc cgcgggaatg
      361 tcggaaaccg ttcgtgagat gattgaacac cggatgatag acgtaatcgt cagcaccggc
      421 gcgaatatgt cgcatgatct cgtcgaggga ttcgggtttc accattaccg gaacaccgat
      481 ctcaagaacg atctcgagct ccgcgaggcg tacatcaacc ggatctacga cacctatctc
      541 gccgaagacg cttttgccgg taccgaggag aagctccttc cgcacctgaa gaattacgac
      601 ggggtcacgt ccgctgattt gacccggtat ctcggcacgg tggtcgaacg accgtgcgta
      661 ctgaaaacgg cgacggaaca cgatgtgccg attttcattc ccgcactgaa tgacagcgaa
      721 cttgggatcg cggtgaaccg ttacaatcat ctcaaccgcg gagggcatca ggttcgatgg
      781 gacggcctgg cagataatga agcctttgcc aacatgatga agcacactga tacgcagggg
      841 attgtcatct gcggcggtgg tgtcccccga aactgggcgc agcaggtaac tccattactc
      901 gaatacctga acttgggtgc cggtgaggag ttcgagcggt cgctacccgg gtacaagtac
      961 ggcatccaga tcaccacgga cgtaccggat tacggcggca tgtcagggtg cacgttcagc
     1021 gaatcgattt cgtggggtaa gtacgacgtg aacgctcgat acgtgcgcgt gagctgtgat
     1081 acgacgatcg cgctgccgct catcgtcgcc gcggcgattg cgcggctcta aacctcggat
     1141 cggagcaaat atatcttggg cgtgagctgg gtcgttatcg cgaatccggt tgcgggaaaa
     1201 ggccgacgta tgcgcggcct cagcgtagcg gtcgacgcgt tgaagcagca gggaatcgac
     1261 acgcaggtcg tgtggacgcg ctgtcccgga gatgggcggg agcgcgcgca cgaagtagcg
     1321 gagcaaggtc acacaaacat tgcggttgct ggtggcgatg ggaccgcaaa tgaggtgctt
     1381 aacggtctcg cggacacggg tcgcctctcg gaattcacca ttacaacgct cccgttcgga
     1441 acagggaatt cgtttctccg cgacttcgac atgaacgatc cacgcgttgc aatcgaccgc
     1501 gtcatccgcg gcgacgctcg tccgtgcgat cttctccgct gcacgattca acg
//
LOCUS       DSCC01000081            2483 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028739, whole genome shotgun sequence.
ACCESSION   DSCC01000081 DSCC01000000
VERSION     DSCC01000081.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028739"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..874
                     /locus_tag="ENN56_00955"
     CDS             <1..874
                     /locus_tag="ENN56_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDS74084.1"
                     /translation="ILWRLLNYNTANNLEEYMLDYHHHSEASVDSETPMADVCRTAIA
                     RGVTEIAFTEHMDFIPEEPNTGYFDYTRYMTAIDAARREFSRDLTVLAGIEIDYCPDF
                     EDEIAQWLSGKEFDFVVGSVHYIRGDGNISEPRALDFFRDRTIDEAYNRYFDLVQQSA
                     ETGMWDALGHLDLIKRYGVTHYGPFDTAPFSDQIDRILTTIIRNGIALEVNTSGLRQN
                     PRDFYPAPTILRRYRELGGARVTVGSDSHTEKHTGEGIAEAYTMLKEIGFHTVEQFRE
                     RNGLSVPIDTLSPS"
     gene            complement(899..2161)
                     /locus_tag="ENN56_00960"
     CDS             complement(899..2161)
                     /locus_tag="ENN56_00960"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS74085.1"
                     /translation="MAKKPASSGQVFTQTATTPLRDLDRWHQIGEEEARVVYEMTLRN
                     ELSGGTETVREFENTFREWVGLRHAISVMNGTSALWSAMYGLKVGPGDEVICPVYTWI
                     CTISAAPMLGAKPVFCDVDPTTMLMDPNDLRKKITRRTKAIIPVHLWGWVCDLDAIMS
                     ISRETGVPILEDCSHAHGATFDGKIVGSIGHVGAWSMQGSKAVSGGECGVIATDDDEI
                     FDRACMIGQVNRVAGLDLVGETYQKYQPLGLGVKFRAHPLGVGIANVQMRKLSKLNEG
                     RRKYLTAVEVGLRDIPGLKPIPTTPKSERGGLYGFPTTFRADELPGVTRADLIAALQK
                     HGVPANDPSGYPLLHRLPLFRDGFDIFGGGRGALCEGYKGYKEGNFPGAEKIYDTIVF
                     LPMLTDPVDGAAEEVVRRIRAAVDSLGG"
     gene            2339..>2483
                     /locus_tag="ENN56_00965"
     CDS             2339..>2483
                     /locus_tag="ENN56_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDS74086.1"
                     /translation="MTESRPNEPDLRVSFAGIELPNPILVASGTFAYGQEVARLYDLS
                     VLGG"
BASE COUNT          527 a          824 c          644 g          488 t
ORIGIN      
        1 catactctgg cgactactca attacaatac tgcgaacaac cttgaggaat acatgctcga
       61 ctaccaccac cactcggaag catccgtcga ttcggaaact cccatggctg acgtctgccg
      121 caccgcaatt gcgcgcggcg tcaccgaaat cgcgtttacc gaacacatgg atttcatccc
      181 cgaggaacca aacaccggct atttcgacta cactcgatac atgaccgcaa tcgacgccgc
      241 tcgacgcgaa ttctcccgcg acctcaccgt tcttgcgggc atcgagatcg actactgccc
      301 cgatttcgaa gacgaaatcg cgcaatggct cagcggcaag gagttcgact tcgtcgtcgg
      361 atcagtccac tatatccgcg gcgacgggaa catcagcgaa ccgcgcgcgc tcgacttctt
      421 ccgcgatcga accatcgacg aggcgtacaa ccgctacttc gatctcgtac aacaatctgc
      481 cgaaacgggc atgtgggacg cgctcggaca cctcgatctc atcaagcgat acggcgtgac
      541 gcactacggt ccgttcgaca ccgctccgtt ttccgaccag atcgaccgga ttctcacgac
      601 gatcatccgc aacggtatcg cgctcgaggt caacacatca gggctgcgac agaatccgcg
      661 cgacttctac cccgcaccaa ccattctgcg acgttatcgc gagctcggcg gcgcacgtgt
      721 aaccgtcggc tcggactctc acaccgaaaa gcacacaggc gaggggattg cggaagcgta
      781 tacaatgctg aaggagatcg gttttcatac ggtcgagcag ttccgcgaac gcaacgggct
      841 gagcgtacca atcgatacac tcagcccgtc atgacgcagg cgtcgattca acgagaaatc
      901 agccgccgag cgagtccacc gcagcgcgaa tccgccggac aacctcctcc gccgcaccat
      961 cgaccggatc ggtgagcatc ggtaggaaca cgatcgtgtc gtaaatcttc tccgcacccg
     1021 ggaagttgcc ttccttgtag cccttgtatc cctcgcacaa cgcgcctcga ccgcccccga
     1081 agatatcgaa tccatcacgg aagagcggta agcggtggag gagcggatac ccactcggat
     1141 cattcgccgg cacgccgtgc ttctggagcg ccgcgatgag atcggcgcga gtcactcccg
     1201 gtagttcgtc tgcgcggaac gtcgtcggga agccatacaa cccgccgcgt tcggatttcg
     1261 gcgtcgtcgg gatcggtttc aatccgggaa tgtcacgcaa ccctacttca accgctgtga
     1321 ggtatttgcg acgcccttcg ttcagcttcg atagcttcct catctggacg ttcgctatcc
     1381 caacaccaag tggatgcgca cggaatttca cgccaagccc gagcggctga tacttctgat
     1441 acgtttcgcc gacaagatcc agaccggcga cgcgattcac ctgaccgatc atgcaggcgc
     1501 gatcgaatat ctcatcgtcg tcagtcgcga tcacgccgca ttcaccgccg ctgacagcct
     1561 tcgagccctg catgctccac gcgccgacat gaccgatgct tccgacaatc tttccgtcga
     1621 acgtggcacc atgcgcgtgt gagcaatcct cgaggatcgg cacgccggtc tcacggctga
     1681 tcgacatgat cgcgtcgaga tcgcataccc aaccccacaa gtgaaccgga atgatcgcct
     1741 tcgtacgccg cgtgatcttc ttccggaggt cgttcgggtc catgagcatc gtcgtcgggt
     1801 cgacgtcgca gaagaccggt ttcgcgccca gcatcggcgc cgcgctgatc gtgcaaatcc
     1861 acgtgtatac cgggcaaatc acctcgtcgc ccgggccgac cttgaggccg tacatcgcgc
     1921 tccacaacgc cgacgtgccg ttcatcaccg agatcgcgtg ccgcaatccg acccactccc
     1981 ggaacgtgtt ctcgaactcg cggaccgttt ccgtgccgcc ggaaagttcg ttccgaagcg
     2041 tcatctcata gacgacacgc gcctcttcct cgccgatttg gtgccagcga tcgaggtctc
     2101 tcaaaggggt cgtcgcggtc tgtgtgaata cctgcccgga agatgccggt ttcttcgcca
     2161 taatgcactc cccggagatg aacggctgtt gatagttgca tcctgtcaat caagggtaat
     2221 ccgcggtgaa tatcgtacca agatatcctc gatgcggtac gttgctcata tggatgtctt
     2281 tgcggtaccc ttcctcttcg acaccgtacc gtctcacccg aaacagcatc gacggaccat
     2341 gaccgaatca cgtccgaacg aacccgatct gcgcgtatct ttcgccggaa tcgaactgcc
     2401 caacccgatc ctcgtcgcgt ccggcacatt cgcctacggg caggaggtcg cgcgactcta
     2461 cgatctctcc gtgctcggcg gac
//
LOCUS       DSCC01000082            3786 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016287, whole genome shotgun sequence.
ACCESSION   DSCC01000082 DSCC01000000
VERSION     DSCC01000082.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016287"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..788
                     /locus_tag="ENN56_00970"
     CDS             <1..788
                     /locus_tag="ENN56_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDS74087.1"
                     /translation="IMGPELMNVMHQQAERFGTHFTYGAITEVDFSERPFRIDLEGTI
                     YHADTFIVASGASARLLGTKGEETLMGYGVSACATCDGAFFRNQDVAVIGGGDSAMEE
                     ASFLTRFAKSVTIIHRRDEFRASQIMQNRVLSHEKIRVIWDSVVDEMVGTREKGLTSL
                     KVRNVKTDEISEVPMQGCFIAIGHDPNTKVFRGKLDMDEKGYLVTYPHANKGAIPATA
                     TNVPGVFACGDVQDAYYRQAITAAGSGCMAAIDAEHFLAEQEG"
     gene            865..1584
                     /locus_tag="ENN56_00975"
     CDS             865..1584
                     /locus_tag="ENN56_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74088.1"
                     /translation="MWRVIRVIAGAITIVCVVLVALSVKRQMIEIDEWELGVPLNAVS
                     ELPPLERTITDLYAEPIAPGRRVRLLEPFSVRDTFHAVRPILSSDGRSIGIPEELVFL
                     EDPTTGMRMAIVRPAGTPTDDWIELMPMPRDVTHAPRFGRWTPLAHWSDIWHPGWADA
                     NNPEPFMWLGMTSDLIRAHDIAGDPTEIPYRVKKQFIAGAPGGPKQPAILTPRAFVLS
                     LVAIVGLFVALHGQRLIGRTT"
     gene            complement(1613..2305)
                     /locus_tag="ENN56_00980"
     CDS             complement(1613..2305)
                     /locus_tag="ENN56_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HDS74089.1"
                     /translation="MRRVGLMVVALAFAASAFAQTREAPAAFTIAQLHYGGGGDWYAN
                     PSSLPNLLAFIRANTNMDVATEPGRVRIMDNDLSNYPFLYMTGHGNVRFSDEEVSRLR
                     RYLASGGFLHADDNYGLDASFRREIKRVFPDKDLVELPFDHPIYHCWFDFPNGLPKIH
                     EHDGKRPQGFGIFHEGRLVVYYTYETDLGDGWEDQHVHNNPEEKRRSALRMGTNVVVY
                     ALTQHGYTAASR"
     gene            complement(2314..3180)
                     /gene="ispH"
                     /locus_tag="ENN56_00985"
     CDS             complement(2314..3180)
                     /gene="ispH"
                     /locus_tag="ENN56_00985"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDS74090.1"
                     /translation="MEIRVAKSAGFCFGVKRAIDIALDTATHSSGRTVQMLGDIVHNE
                     RVVEQIDEAGVKVIDNIEHADRNGVLLVRAHGTGPDTYADARKRGLDVVDATCPLVTD
                     IHEKARELHEQGYPIIVIGDHGHDEVRGIAAQVPNAIVVATPDEVGKLGRRFRRVGVV
                     CQSTQNVENVQAIIAALVPICIDLHFVNTICYPTTKHQAEIRTMPLENDVMIIVGSFT
                     SANTKRMTEISRSLNPRTHQVTGPEDLDSSWFDGVTTVGVHAGASTPDFAIDEVVESI
                     RSMVSEPTNVIG"
BASE COUNT          825 a         1067 c         1066 g          828 t
ORIGIN      
        1 gaattatggg gccggagttg atgaatgtca tgcatcaaca ggcggagcgc ttcggcacac
       61 acttcacata cggtgcgatc actgaagttg acttctccga gcgtccgttc cgtatcgatc
      121 tcgaaggaac catctaccac gctgatacat tcatagtcgc atctggcgca tcggcccgac
      181 tcctcggaac caaaggcgaa gagacgctca tgggctacgg cgtatcggcg tgtgcgacat
      241 gtgacggtgc gttcttccgg aatcaagacg ttgcagtgat tggtggcggc gactcagcga
      301 tggaagaagc ctcgttcctc acgcgtttcg cgaagagcgt tacgatcata catcgccgtg
      361 atgagttccg cgcatcgcag atcatgcaga accgcgtgct ctcgcatgaa aagatcagag
      421 tgatctggga cagtgtcgtc gacgaaatgg tggggacccg tgagaaggga ttaacctcgc
      481 tcaaggtgcg taacgtcaag acggatgaaa tttcagaggt accgatgcag ggttgtttta
      541 ttgccatcgg tcatgatccg aacacgaagg tattccgcgg aaaactcgat atggatgaaa
      601 agggatatct cgtcacgtat ccacatgcga acaaaggcgc gattccagcg acggcgacga
      661 atgtccccgg cgtattcgcg tgtggcgatg ttcaggatgc gtattaccga caagccatca
      721 ccgccgcagg tagcggttgt atggcggcta tcgacgccga acacttcctt gccgaacagg
      781 aaggctaatc acgccggtac aaagaacggt cgtcgcagta ttcaaaagcg acgaccgttt
      841 tttctttgcc tcaggagtat ctccatgtgg cgcgtaatca gggtaatcgc gggggcaatc
      901 acgatcgttt gcgtggtctt ggtcgcactg agcgtaaagc gccagatgat cgaaatcgat
      961 gaatgggaac tcggcgtgcc actcaacgcg gtgtcggagc ttccaccgct cgaacgaacg
     1021 attactgacc tttacgccga accgattgca cccgggcgcc gtgtccgtct actcgaaccg
     1081 ttttcggtcc gcgatacctt tcacgccgtt cgacccattc tttcatcgga tggccgtagt
     1141 attggaattc ccgaagagct cgtatttctc gaagatccca caaccggcat gcgcatggca
     1201 attgtccgac ctgcgggcac gccaacagac gattggattg agcttatgcc catgccgcgt
     1261 gacgtgacac atgcgccgcg attcggccga tggacaccgc tggcacattg gtccgatatc
     1321 tggcatccgg gttgggccga cgcaaacaat cccgaaccgt tcatgtggct tggaatgacc
     1381 tcggacttga tccgcgcaca cgatatcgcc ggagatccga ccgaaatccc ctatcgcgtg
     1441 aagaagcaat tcatcgcggg tgcgcccggc ggtccaaaac agccggcgat cctcacgcct
     1501 cgcgcgtttg tcctgtcgct cgtcgcgatc gtcgggcttt tcgtggcact acatggtcag
     1561 cgtttgatcg gacgcaccac gtagaggaga ccacaatagt gccgacgcaa cgtcatcgag
     1621 acgccgcggt gtatccgtgt tgggtaagcg cgtaaacgac gacgttcgtc cccatgcgaa
     1681 gggccgagcg acgcttctct tccgggttgt tgtgaacgtg ttggtcttcc cacccgtcac
     1741 cgagatcggt ctcgtaggtg taatacacga cgagtcgacc ttcgtgaaat atgccgaatc
     1801 cctgaggacg tttcccgtcg tgctcgtgga ttttcggtag cccgttcgga aagtcgaacc
     1861 agcagtggta tatgggatga tcgaacggta gctcgaccaa atccttgtcg gggaagactc
     1921 gtttgatctc gcgacggaag cttgcgtcga gcccgtagtt gtcgtctgca tggagaaacc
     1981 cgccactcgc gagataccgt cttaaccgcg atacctcctc atccgaaaag cgaacgtttc
     2041 cgtgaccggt catgtagagg aaggggtaat tcgagaggtc gttatccatg atccggacgc
     2101 gtcccggttc cgtcgcaaca tccatgttcg tgttcgcacg aatgaacgcg agcaggttcg
     2161 gtaggcttga cggattcgca taccaatcgc ctccgccacc gtagtggagt tgcgcgatgg
     2221 taaaggctgc aggtgcttcg cgcgtttgcg cgaacgccga tgccgcgaac gccagcgcga
     2281 caaccatcag tccgactcgc ctcattgcga ttctcatccg attacgtttg ttggttcgct
     2341 taccattgaa cggatcgatt cgacgacctc gtcgatcgcg aaatccggcg tcgaggctcc
     2401 agcgtgcaca ccgacggtag tcacaccgtc gaaccatgac gaatcgaggt cttccggacc
     2461 cgtaacttga tgggtccgag ggttcagcga acgagaaatc tccgtcatcc gcttggtatt
     2521 tgcactggta aaggatccga cgatgatcat gacatcgttc tcgaggggca tcgttcggat
     2581 ttccgcctga tgcttcgtcg tcggatagca gatcgtgttg acgaaatgga gatcgataca
     2641 aatcggcacg agtgccgcga taatcgcctg gacgttctcg acgttctgcg tggactgaca
     2701 cacgacgcct acgcggcgga atcgccgacc gagtttgcca acctcgtccg gcgtcgcaac
     2761 aacgatcgcg ttgggaacct gcgcggcaat accgcggact tcatcgtggc cgtggtctcc
     2821 gataacaatg atcgggtacc cctgttcgtg cagctcgcga gccttttcat gaatgtctgt
     2881 cacgagcgga caggtcgcgt caacaacgtc gagtccacgc ttcctcgcgt cggcgtatgt
     2941 atcggggccg gttccgtgtg cgcgtacgag aagcacaccg ttgcgatcgg cgtgttctat
     3001 gttgtcaatg acctttacgc cggcttcatc gatctgttcc actacgcgtt cgttatgaac
     3061 gatgtcgccc agcatttgca ccgtgcgacc gcttgaatgc gttgccgtat ccaacgcgat
     3121 atctattgcg cgtttcactc cgaagcagaa tccagcactt ttcgctacgc gtatttccat
     3181 ggtctcctcg cgaatccatc aaaatcgtga tgccatgcga ttctcggtat gagttactcc
     3241 tcgcatcagt gtacgttccc ggttataacg cgaaaacctc cgcaatggtt cgtttgacgt
     3301 cgacgaacgc acaacgctgt atactttgta tcagatagac aaacgctctc tttcactgga
     3361 ggagggggac gtgaaacgaa aaacgtatac tacaggactc gtgtgcgtgt gcttcgttgc
     3421 gcttactgca tgttccgcgt cccgatcccc gtcatcgagt cccgttgagc cggatgatgg
     3481 tccgcccgca caaatcgtcc gaaccgagcc gcccgaatac cgtcatatcc ctcagggcgc
     3541 atggacgagt gcaacaaaat cgaaagcgcc acagcagcgt tcgccgcgat cgaccgacgg
     3601 atcgtcgaat acggtggttt acttggagcc actgacggta accgaagccg caccgaatcg
     3661 agtagaggaa aacgaatcgg aagaacccga gaacgacggc gattagccga tcgttcgcaa
     3721 cgaaagttcg acgacgccga tcacgtaccg gggaccgggg ccggcggaac acgctatggc
     3781 gggatc
//
LOCUS       DSCC01000083            2267 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032444, whole genome shotgun sequence.
ACCESSION   DSCC01000083 DSCC01000000
VERSION     DSCC01000083.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032444"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="ENN56_00990"
     CDS             complement(<1..1802)
                     /locus_tag="ENN56_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74091.1"
                     /translation="MAAFVPSALIRSRITAMVFVATLIGGTHVMALEFVDVTGAVVGV
                     EPADSESATFAATVLIEEVERRTGVRWAIDGEVTEGMPRIDVVLDTADASLGDEGYRI
                     GVDLIETWVSGPTRVRISATTRRALLFGVGRFLREADCTEGVVLFPTAGLIESRPAQR
                     LRGHQLGYRPLPNSYDAWSVEQFDQYIRELVFFGTNAIESIPTSTEEGARINKHMIMT
                     REEMNVHISELCQKYDIEYWAFAPAMVDLSKRNEKRQLLRDHEAFYRSAPHISAVFVP
                     GGDPGTNHPQHLYPYLAELSALLKRYHPNAGIWTSLQKFEPDKAEWTYAYLNREQPDW
                     LAGIVSAPWDPGIEELRARLPDRYPIRVYPDITHNIRSQFPVPWWDPALNMTLGREAP
                     NPRPYDHSIIHEAYTRGSIGFLAYSEGIHDDVNKIVWSLRGWDRDMPVIEIVREYARF
                     FFGPDVAERAADGILALEENWRGPLATNGGVEATLALWQTLEGEHPELQNSWRWQLAL
                     LRAYFDAYVRSRLIRESTLEAEAMAALATASDIGPDAAMSEAERLLAKATEEPIRPDL
                     RQRIEEITHDMFRAVGYQTSVPKYGAHGPERGAIL"
     gene            complement(1913..>2267)
                     /locus_tag="ENN56_00995"
     CDS             complement(1913..>2267)
                     /locus_tag="ENN56_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDS74092.1"
                     /translation="AGDWGENLAVDHLVRKGYDVVARNWRAGRLGELDIVARDGDALV
                     IVEVKTARGRAMGHPATWVTPPKQARIERLAEVFLAECVDRYEAVRFDVVTVDASVRP
                     ARIEHVVDAWRPTES"
BASE COUNT          453 a          751 c          619 g          444 t
ORIGIN      
        1 aggattgcac cgcgttccgg cccgtgcgca ccgtatttcg gaacactcgt ctgataaccg
       61 actgcccgaa acatgtcgtg tgtgatctct tcaatccgct gccgaaggtc cgggcgaatg
      121 ggttcctcag tcgccttcgc gagtagacgc tccgcctccg acatcgctgc gtccggtccg
      181 atatcagatg ccgtcgcaag tgccgccatc gcctccgcct ccaacgtcga ctcgcggatc
      241 agacgactcc gcacgtacgc gtcgaagtat gcgcgaagga gtgcgagctg ccaccgccac
      301 gagttctgca gctccgggtg ttcgccctcg agcgtttgcc agagcgcaag cgtcgcttcg
      361 acaccaccgt tcgtcgcaag cggtccgcgc cagttctctt ccaacgcgag aatcccgtcc
      421 gcagcgcgct cggcgacgtc cggcccgaag aagaatcgcg catattcacg cactatctcg
      481 atcaccggca tgtcacggtc ccaaccgcgg aggctccaga caatcttatt cacgtcgtcg
      541 tggatacctt ccgagtacgc gagaaacccg atcgaaccgc gcgtgtatgc ttcgtggata
      601 atcgagtgat cgtacggccg cggattcggc gcttcgcgac cgagtgtcat attcagcgcc
      661 ggatcccacc acggcacggg gaattggcta cggatattgt gcgtgatgtc cggatacacc
      721 cgaatcggat accgatcggg gagacgcgca cggagttcct cgatgcccgg gtcccacgga
      781 gccgatacga tacccgccag ccagtcgggc tgctcccgat tgaggtacgc gtaggtccac
      841 tcagccttgt ccggttcgaa cttttgcagg ctcgtccaga tacccgcgtt cggatgatag
      901 cgcttcagga gcgcgcttaa ctcggcaagg tacgggtaca gatgctgcgg atggtttgtt
      961 ccgggatcgc cacccggtac aaacactgcg ctgatgtgcg gtgcgctccg gtaaaacgct
     1021 tcatgatcgc gaagaagctg acgtttctcg ttccgcttgg agaggtcgac cattgccggt
     1081 gcgaatgccc agtactcgat gtcgtatttc tgacacaact cgctaatatg aacattcatt
     1141 tcttcgcgcg tcatgatcat gtgcttgttg attcgcgcgc cttcttcagt cgaggtcggg
     1201 atactctcaa tagcgttcgt cccgaagaac accagctcgc ggatgtactg gtcgaattgc
     1261 tccacgctcc acgcatcgta cgaattcggt aacggccgat acccaagctg atgcccgcgc
     1321 aaccgctgcg ccggacgcga ctcgatcaaa ccggcggtcg ggaacagtac gacgccttcc
     1381 gtgcaatcgg cttctcggag aaatcgaccg acgccaaaca gcaacgcgcg acgtgttgtg
     1441 gcgctgatgc gcacgcgagt cggaccagac acccacgtct cgatgaggtc aacgccaatg
     1501 cgataccctt catccccgag cgacgcgtcc gcagtatcca aaacaacgtc gatgcgcggc
     1561 atcccctcgg ttacctcgcc gtcgatcgcc caacggacgc ccgttcgccg ttcgacctct
     1621 tcgatcaaca ccgtcgcagc gaaggttgcc gactccgagt cagccggctc gacaccgacg
     1681 accgcaccgg tgacatcgac gaattccagc gccatgacgt gtgttcctcc gatgagtgtg
     1741 gcgacgaaaa ccatcgccgt gattcgcgat cgaatcaatg cagacgggac gaatgcggcc
     1801 atatcgacct ccgtggcaat gagccagctt cgatgggaat atagtatacc tacaacagac
     1861 taatgtcggg cgcaaggatg ttcgtaccgg tatgaacccg gtcgcgcgat tcctacgatt
     1921 ccgtcggccg ccacgcgtcg acaacgtgct cgatacgcgc cgggcgaacc gacgcgtcaa
     1981 cagtgacgac atcgaatcgc accgcttcgt atcggtccac gcattccgca aggaatacct
     2041 ccgccagacg ctcgatccgc gcctgtttcg gcggagtaac ccacgtagcc ggatgcccca
     2101 tcgcccgacc tcgtgcggtc tttacctcga cgattaccag cgcatcgcca tcccgcgcaa
     2161 cgatatcgag ctccccgagt cgtcccgctc gccagttcct cgcgacaaca tcgtacccct
     2221 tccgcacgag atgatcgacc gccagattct ctccccagtc tccggcg
//
LOCUS       DSCC01000084            2762 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024951, whole genome shotgun sequence.
ACCESSION   DSCC01000084 DSCC01000000
VERSION     DSCC01000084.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024951"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..139)
                     /locus_tag="ENN56_01000"
     CDS             complement(<1..139)
                     /locus_tag="ENN56_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase"
                     /protein_id="HDS74093.1"
                     /translation="MPISDEHLIKRAIDGNHVAFAILVERHRDAVTRVVAEIVRPGAD
                     LE"
     gene            415..1506
                     /locus_tag="ENN56_01005"
     CDS             415..1506
                     /locus_tag="ENN56_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74094.1"
                     /translation="MPRDIPLRISRIFIRSPLCAFVLAVALGGCSTDVVDTPITDIGD
                     LETPVALRTWLVAGPLPSPDLGTRRSNQIPARAGFDLDYLAAVGGETNYRATEGAEFP
                     GPLGDSYLFRRFTWNTPYIDLTELYGRPANVAAYLYTEIESDEPRHAYFHIGLNDAGK
                     VWLNGELVLSYPIDTPARRSERVVSVDLPAGRSHILVKIDQAGANWGGYFELAKTTTL
                     SDVDSTPRPGRSSSIFPHSSRAYVSLWIIGVFVVSPVAFVIAILIYVRYRREADKAYV
                     ENLTTMMERGVPPHQAKALLTTTKEPSTHPARSAFFAGLALMLGGLGLTATEIVHAGF
                     GNAGFELFVMFTGVALLASYFIVDRRKSE"
     gene            1638..>2762
                     /locus_tag="ENN56_01010"
     CDS             1638..>2762
                     /locus_tag="ENN56_01010"
                     /inference="COORDINATES: protein motif:HMM:PF03747.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDS74095.1"
                     /translation="MTEAIKLLKDEITQRREEGCDERALAELKRAIDKLPDTAPQQTV
                     EDLYLRLDRLQPDPTLAAKEPSDLDGIRALRPDGPRELDLGLSDEQLTDRMLGAWLAR
                     AAGCTLGKPVEGWPRGDIRALLESAGEYPLRHYFPALSDYPRKWHPGNIGCLRGNITR
                     MERDDDMDYTFLGLRVMEHHGPEFTTDHVARAWLDELPYFRVYTAERVAYRNLVIEKS
                     VPETALWRNPYREWIGAQIRADMWGYVAAGMPELAAEFAWRDAALSHVKNGIYGEMWM
                     AATIAAAFCVTDMAEAIRIGMSEIPAECRLADALRDVLAWYEDDSDRERLLDRIHETY
                     GHYHPVHTINIAALVAAGLLVGENNLGTTISTAVMGGWDTD"
BASE COUNT          551 a          862 c          808 g          541 t
ORIGIN      
        1 cttcgagatc ggcgcccggg cgaacaatct ctgcgacgac gcgcgtcacc gcgtctcgat
       61 gtcgctcgac gagaatcgcg aacgcaacgt ggttaccgtc gattgcgcgt ttgatgagat
      121 gttcgtctga tatgggcatg gagaacacct acccagcgta ctcacactga tgaagaccga
      181 ggcatatgcg aaaaggttgc gctcgtttga tgtgggcgca cgcttgggct tctgtcgtga
      241 ggttacacgg aatgtacgtc caacacaata cgacgggaac ccatcgcgca ttgtcctgca
      301 acacttcgag tgctccgcag gtctattcgt aagaccgcac ctttcatggt gcggtccgcc
      361 gccaacctat catgcggtag cggtgggacc tgttacgtga gaaggagcgc caccatgccg
      421 agggatatcc ctctgcggat cagtcggatc ttcattcgtt cgccgctgtg cgcattcgtc
      481 ctcgccgttg cactcggcgg atgctccacc gatgtagtcg acacgccgat taccgatatc
      541 ggtgatctcg aaacgccggt cgcgcttcgg acgtggctcg ttgccggtcc gctgccgagt
      601 cccgatctcg gtacgcgtcg atcgaatcag atccccgcac gggccgggtt cgacctcgac
      661 tacctggcag cagtcggtgg tgagaccaat taccgcgcga cggaaggtgc agagttcccc
      721 ggtccgcttg gcgactcgta cctcttccgc cggtttacgt ggaatacgcc ctacattgac
      781 cttaccgagt tgtacgggcg tccagcgaat gttgcggcct acctctatac agaaatcgaa
      841 agcgacgaac cgcgccacgc gtacttccac atcgggttga acgacgcagg gaaagtctgg
      901 ctgaacggcg agctcgtcct ctcgtacccc atcgacacgc ccgcgcgtcg ttcggaacgg
      961 gtcgtcagcg tcgatctgcc ggcggggcgc tcgcatatcc ttgtaaaaat cgatcaggct
     1021 ggcgcgaact ggggcggata cttcgagctt gcgaagacga cgacgctttc ggacgtcgat
     1081 tcgacacctc gtcccggccg ttcgagcagt atcttcccac actcgagccg ggcatacgta
     1141 tcgctctgga tcatcggcgt cttcgtcgtt tctcccgttg cgttcgtcat cgcgatcctc
     1201 atctacgtgc ggtaccgccg cgaagccgac aaggcgtacg tcgaaaatct caccacaatg
     1261 atggaacggg gcgttccgcc gcatcaggcg aaagctctcc tcaccacgac gaaagaacct
     1321 tcgacgcatc cggcgcgcag tgcgtttttc gccggactcg cgctgatgct tggaggactt
     1381 gggcttacgg cgacagaaat cgtccacgcg ggcttcggta acgccggctt cgagctcttc
     1441 gtgatgttca ccggtgtcgc gctccttgcg tcgtatttca tcgtcgatcg tcgtaagtcg
     1501 gaataacgca accgaatcga cagactcatc ttggggcgac ggatcggcgg aaacgcggga
     1561 acgtcgccct tttcgcatcc aacaccgtac ggtaccattc ttcgcatgac accatccatc
     1621 gaaccggtga ggatcgtatg accgaagcca tcaaattgct caaggacgaa atcacccagc
     1681 gccgcgaaga gggctgcgac gagcgcgcgc tcgcagaact caaacgcgcg atcgacaaac
     1741 tccccgatac tgcgccgcaa cagaccgtcg aagatctcta tctccggctc gaccggctcc
     1801 aacccgatcc gacgctcgcc gcgaaagagc cttccgacct cgacggaatc cgcgcgttac
     1861 gtcccgacgg accgcgcgag cttgatctcg gcctgagcga cgaacagctc acggaccgta
     1921 tgctcggcgc atggcttgca cgtgcggccg gctgcacact tggaaaacct gtcgaggggt
     1981 ggccgcgagg cgacatccgc gcactgctcg aatcggccgg cgagtatccg ctccgccact
     2041 acttccccgc actcagcgat tatccgagaa agtggcatcc gggcaacatc ggctgcctgc
     2101 gtgggaacat cacgcggatg gaacgtgacg acgacatgga ctacacgttc ctcgggcttc
     2161 gcgtgatgga gcaccatgga ccggagttca cgactgatca tgtcgcgcgc gcatggctgg
     2221 atgaactgcc gtatttccgc gtctacaccg ccgaacgtgt cgcgtaccga aatctcgtta
     2281 tagaaaagtc ggtaccagag accgcactat ggcggaatcc ctaccgcgag tggatcggcg
     2341 cgcagattcg tgccgacatg tggggctacg tggccgcggg tatgccggag cttgccgcgg
     2401 agttcgcgtg gcgcgatgcc gcgctcagtc acgtgaaaaa cggcatctac ggcgagatgt
     2461 ggatggccgc cacaattgcc gcggcattct gcgtaactga catggccgag gcgattcgaa
     2521 tcggcatgag cgagataccc gccgagtgtc gtctcgcgga tgcgctccgt gatgttctcg
     2581 cgtggtatga agacgattcc gatcgcgaac ggcttctcga ccgaattcac gagacgtacg
     2641 gtcactatca cccagtccac accatcaata tcgcggcgct cgtcgcagcg ggacttctcg
     2701 tcggcgagaa caacctcgga acgacgatct ccacggcggt catgggcgga tgggataccg
     2761 ac
//
LOCUS       DSCC01000085            2251 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032767, whole genome shotgun sequence.
ACCESSION   DSCC01000085 DSCC01000000
VERSION     DSCC01000085.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032767"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..259)
                     /locus_tag="ENN56_01015"
     CDS             complement(<1..259)
                     /locus_tag="ENN56_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74096.1"
                     /translation="MSAHQPSIRRTEYREPVWEIPPPPDGDAVRRISERWKLSRVVAK
                     LVAQRIGAGNDDVAIDRFLNPRFDHLHDPYLMKGMYEAVTRL"
     gene            complement(290..2149)
                     /locus_tag="ENN56_01020"
     CDS             complement(290..2149)
                     /locus_tag="ENN56_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74097.1"
                     /translation="MNPLRFRWFSLAAMIVAAVSSVRAGDYGLLFSHDLNVWSWDQSI
                     GWDRVFGDKLVVTANGSHTRKVSESSAYDQRSDFATGNLRFNYSVTPQWSLGSEFTID
                     QSRLTAGAVRSDVISRRAELDSRYELYPGVQVRGAAGAAYEERRDVAHAGFAWRVGAT
                     AYPFRHVRLGDRANIVVTFDRDAVESPQGDAQTSFAVQGGMRWTPWLDQTVQYSENHD
                     AQRYVSAFDGGRILLRVNHSRKVRTELDGVYPLVGLVELEGEYRWGDVQDDASNDSTS
                     LKYRTDNTTHSLSGGVTWHIPLERPNLTYRVTSSRSTRDAVPVVKSDSLVLRNDLDRH
                     RNDLGMSLASVFAFGRTDSLTLSGSLAISRDHTPAETEVNDRDDFTRTFVALYHHGFR
                     SGTTLQLRAERIETHQVWLNRARSANNRWDRALNLWATTRAPLGIITFTQRAFFKAQL
                     EEFDFDYLTPDRPRSRNARIGRLDFIGEFSLPGDGLATAEYTIEARTRGVLLPADRPG
                     ERPTLWQLTRNELAQVVTVDLGVPIGERWTFSPEVSVSRQLDFIPTQTAWNPFRLGRQ
                     VDKQEALYLEATALYRPDGDLGAFSVRAARTYRRRGAITDTRDFVSVTWQYAF"
BASE COUNT          480 a          681 c          630 g          460 t
ORIGIN      
        1 caagccgtgt cactgcctcg tacatcccct tcatcaggta cgggtcgtga aggtggtcga
       61 atcgtggatt gagaaaccga tcgatcgcta cgtcgtcgtt ccccgcaccg atacgctgtg
      121 cgacaagctt cgcgacaacg cgagatagct tccaccgttc ggaaatccta cgcaccgcgt
      181 cgccgtccgg tggcgggggt atttcccaaa ctggttctcg atactccgtc cggcgtatgg
      241 acggctggtg tgctgacata ctcgcgttcc cttcacgatg tatccgtatt caaaacgcat
      301 attgccatgt gaccgacacg aagtcacgcg tatccgtaat cgcgccacgc cgacggtagg
      361 ttcgcgccgc gcggacggag aacgcgccga ggtcgccatc cgggcggtaa agcgctgtcg
      421 cttcgaggta caacgcttcc tgcttgtcta cctgcctacc gagccgaaac ggattccacg
      481 cagtctgcgt cgggatgaaa tcgagttggc gactaactga tacctccgga gagaacgtcc
      541 agcgttcacc gatcggaact ccgagatcaa cggtgacgac ctgcgcgagt tcgttgcgcg
      601 tgagttgcca taacgtggga cgctctcccg gacgatccgc cggcaggagt acgccacgag
      661 tccgtgcttc gatcgtgtac tcagcggttg ccaaaccgtc acccggtaac gaaaactcgc
      721 caatgaaatc cagtcgcccg atgcgtgcgt ttcggctgcg cggccgatca ggcgtgaggt
      781 aatcaaaatc gaactcttcg agttgtgctt tgaaaaatgc ccgctgcgta aaggtgataa
      841 tgccgagtgg cgcacgggtc gtcgcccaaa gattcagcgc gcgatcccat cggttatttg
      901 ccgaccttgc gcggtttagc catacctgat gggtttcgat ccgttccgcg cgaagctgca
      961 gtgtcgtacc tgatcgaaaa ccgtggtgat agagtgccac aaacgtgcgt gtaaagtcat
     1021 cacggtcgtt tacttccgtt tccgcaggag tgtggtcgcg tgatatggca agtgaaccac
     1081 tcaaggtaag cgaatcggtc ctgccgaatg cgaaaactga ggcgagcgac ataccgagat
     1141 cgttacggtg tcggtccaaa tcgtttcgca aaaccagcga atcgctcttg accaccggaa
     1201 cggcgtcgcg ggtacttcga ctcgatgtta cgcggtaggt aaggttcggt ctctccagcg
     1261 ggatgtgcca cgttacgcct ccgctcaacg aatgagtcgt gttgtccgtg cggtacttca
     1321 ggctcgtcga atcattgctc gcatcatcct ggacgtctcc ccatcggtat tcgccctcca
     1381 gctccacgag cccgacgagt gggtacactc cgtcgagttc cgttcgcacc tttcgactgt
     1441 gatttactcg gaggagaatc cggccgccgt cgaacgcgct tacgtacctc tgcgcatcgt
     1501 ggttttcgct gtactgaaca gtttggtcca gccacggagt ccaccgcata ccaccttgca
     1561 cggcaaacga tgtctgcgcg tctccctgtg gcgattcgac ggcgtcacga tcgaacgtta
     1621 ccacgatgtt tgcgcgatcg cccaaccgta cgtggcgaaa cgggtatgcc gtagccccga
     1681 cacgccatgc aaaacccgcg tgtgctacat cacgacgctc ttcgtacgct gcaccggcgg
     1741 caccgcggac ctgaacaccc gggtagagct cgtaccgaga atcgagctcc gcacgacggc
     1801 tgatgacgtc agagcgcacc gcaccagcgg tcaaacgcga ttgatcaatg gtgaactcgg
     1861 aaccgagaga ccactgtggt gttacggagt aattgaatcg aagattcccc gtggcaaagt
     1921 ccgaacgctg atcatacgcg ctcgattcgg aaacttttcg cgtgtgtgag ccgttcgccg
     1981 tgacgacgag cttgtctccg aagacgcggt cccaaccaat gctctgatcc catgaccaca
     2041 cattgagatc gtggctaaac aagagaccat agtccccggc gcgcaccgac gaaaccgccg
     2101 ctacaatcat cgcagcgagg gagaaccatc ggaatcgcag cggattcacg ccgttcgctc
     2161 cgaaaagaca ccgagcgact gaaacgcgtc ggcaagcgcg atgaactcaa ctgcgccgag
     2221 cgtttcagcc cgtgccgaga gatctatacc c
//
LOCUS       DSCC01000086            1684 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048653, whole genome shotgun sequence.
ACCESSION   DSCC01000086 DSCC01000000
VERSION     DSCC01000086.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048653"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            21..317
                     /locus_tag="ENN56_01025"
     CDS             21..317
                     /locus_tag="ENN56_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74098.1"
                     /translation="MTAIGIKMRTRDVESPAEGPMGFLLAERMIGTLYGVASTVCPGA
                     VFLSASYGIDGTGEITPEPHDPGLTSAFTSALAAASAQHEEVSAAMKQSDRENL"
     gene            314..>1684
                     /locus_tag="ENN56_01030"
     CDS             314..>1684
                     /locus_tag="ENN56_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74099.1"
                     /translation="MNRYLWNVRYSMRGIVFSAILLFLASTGAFASSAITVPIELTAG
                     NTTIQHAAVSIDADLNCHALWIDDTGQLLTRGGPYGTFGATRVVKRNATHLVSDPAVA
                     VLASGYAALIWSARRPEYDWDWNLWFTFSPTSDPIQIIGDTVNDRHPSALARGDSLYI
                     AWQRDGPPYRVAYGFATRARPYPPIYFLESFSDSVSNVRLGGAIGPTVWVAWNVHAET
                     GTEIWLAGLRRDVIHSAPVRLRAGWNDRNPHIAADPAGRPHVVWEDSGSIRYTYGVLD
                     TAGRVVSFAPSTLITASGGSHPSIAFMPNGVAHISWFDTVEDAIVAVTLVGGNVVGPV
                     TLVEPRYQPSLETMASGLLATSAGAIIAPFVGVAPTNGAQGIWIAPYAEVSSNAFFAS
                     FGAEVDATGAVRLTWSTEAQTVARYRIVRMQRDAEPVFIDLATVGGAGTEFAVIDHPG
                     AGGSYQY"
BASE COUNT          311 a          502 c          522 g          349 t
ORIGIN      
        1 atcaacatga gcatagcgcc gtgacggcga ttggcatcaa gatgcgaacg cgcgacgtcg
       61 aaagtccggc ggaagggccg atgggctttc tcttggctga acggatgatc ggcacgctct
      121 acggtgtcgc gtcgacggta tgtccgggtg cggttttcct ttcggcatcg tacggcatcg
      181 acggcacggg tgagatcact cccgaaccgc atgatcccgg gctcacgtcg gcattcacgt
      241 cggcactcgc cgccgcgtcc gcacagcatg aggaagtgag cgccgccatg aaacagtcgg
      301 accgcgaaaa cctgtgaacc gatacctctg gaacgtccgg tattcaatgc gcggaatcgt
      361 tttctcggcc atccttctct ttcttgcttc gaccggtgca ttcgccagtt cggcgatcac
      421 cgttcccatc gaattgaccg cgggcaatac gacgattcaa cacgccgcag tcagtatcga
      481 cgctgacctg aactgtcacg cgctgtggat tgacgacacc ggccaacttc tcactcgcgg
      541 cgggccatac ggcacgttcg gtgcgacgcg tgttgttaaa cggaacgcta ctcatcttgt
      601 gagtgatccg gctgttgcgg tgctcgcatc tggatacgcc gcgttgatat ggagcgctcg
      661 acgaccggaa tacgattggg attggaacct ctggtttacc ttttcgccaa cgtcggaccc
      721 gatccagatc atcggcgata cagtaaacga ccggcacccg tcggcacttg cacgcggcga
      781 ttcgctctat atcgcatggc aacgcgacgg ccccccctat cgtgtggcgt acggcttcgc
      841 gacacgcgcg cggccgtatc caccgatcta cttccttgaa tcattcagtg actccgtgag
      901 taacgtgcga cttggtggag cgatcggtcc gacagtgtgg gttgcgtgga acgttcatgc
      961 ggaaaccggt acggagatct ggctcgccgg tctccgtcgc gacgtgattc acagcgcacc
     1021 cgttcggctt cgcgcgggat ggaacgatcg gaatccgcac atcgccgccg atccagcggg
     1081 gcgtccgcac gtcgtatggg aggattccgg ctccattcgc tacacgtacg gcgtgcttga
     1141 taccgccgga cgcgtcgtct cattcgcgcc gagcacgttg attaccgcgt ccggaggaag
     1201 tcacccgtcg attgcgttta tgccgaacgg tgtcgcgcac atcagctggt tcgacaccgt
     1261 tgaggacgcg atcgtcgcgg tgacactggt gggcggaaac gtcgttgggc cggtaacgct
     1321 ggtggagccg cggtaccaac cttcgctgga aaccatggca tcggggctgc tcgcgacgag
     1381 tgccggtgca atcatcgctc cgttcgtcgg agttgcgcca acgaatggcg cgcaaggcat
     1441 atggattgcg ccctacgccg aggtgtcaag caacgcgttt ttcgccagtt ttggtgcgga
     1501 ggtcgatgcg acgggtgcgg tgcgattgac atggtccacc gaagcgcaaa ccgtcgcgcg
     1561 gtatcgcatt gtgcgaatgc aacgcgacgc cgaaccggtg tttatcgatc tcgccacagt
     1621 gggcggagcc ggtaccgagt tcgcggttat cgatcatccg ggagccggcg gatcgtatca
     1681 gtac
//
LOCUS       DSCC01000087            1567 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053647, whole genome shotgun sequence.
ACCESSION   DSCC01000087 DSCC01000000
VERSION     DSCC01000087.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053647"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..435
                     /gene="tmk"
                     /locus_tag="ENN56_01035"
     CDS             <1..435
                     /gene="tmk"
                     /locus_tag="ENN56_01035"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDS74100.1"
                     /translation="LMLYLASRAQLVAEVVRPALAAGSVVISDRYADSSVAYQGYGRE
                     LGVDVVRQSNALATGNLQPDLTLVIDIPVEITKERMNGKTADRLESEKRSFHERIRNG
                     FLEIARDEPERVVVVDGTKSPDDVHLEIVRIVSIRFPELTSG"
     gene            522..>1567
                     /locus_tag="ENN56_01040"
     CDS             522..>1567
                     /locus_tag="ENN56_01040"
                     /inference="COORDINATES: protein motif:HMM:PF13180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDS74101.1"
                     /translation="MSLRGRDWSWVVLMAVVVIWGGQIFNRVDAAAKDVYSTIARMAD
                     DRAEVENAILQYYVEEIDPVKLQLAEIDGMMNELDTFSDFLDEREQESLRETTEGAFG
                     GLGIQIYPVDHYPTVIAPLEGTPAWDVGLQSGDQIIAIEGESTRDEKLDIIVGKLRGV
                     PGTEVTITIKRPGVGEPIDFTITRAEIHINSVGFSGLVWDPTAQDLPLAAAPDWLRDG
                     EDIGYVRLKTFGRGAADEVRAALEQINARNPRGVVLDMRMNPGGLLEEARAVADLFLP
                     RGQMIVSTKGRSRWSEARLYSEREPVLPTNVPLVVLVDEGSASASEIVAGAIQDNDRG
                     LVIGRNTYGKGSVQT"
BASE COUNT          345 a          413 c          490 g          319 t
ORIGIN      
        1 ctcatgctct accttgccag ccgggcgcaa ctcgttgcgg aagtggttcg tcccgcactt
       61 gcggcgggat cggttgttat cagtgaccga tacgcggact caagtgtcgc gtaccaaggc
      121 tacggacggg aactcggtgt ggacgtggtg cgtcagtcga atgcgttggc gacgggaaac
      181 ctgcaaccgg acttgaccct ggtgatcgat attcccgtcg aaatcacaaa ggaacgaatg
      241 aacgggaaga ccgccgaccg gttggaatcg gaaaagcgtt cattccacga acggattcgg
      301 aacggcttcc tcgaaatcgc acgcgatgag ccggagcgcg tcgtcgtggt cgacggcacg
      361 aaatcgccgg acgacgtcca tttggagatc gttcggatcg tatcgatacg gtttcccgaa
      421 ctcacgtcag ggtaataccg acacgttgac cgagtgcaca accgagtgtt cttttctgag
      481 aacggttttc atttaatcat tcagccgcgg aggcgcagta tatgtcgtta cgaggacggg
      541 attggagttg ggtcgtactc atggccgtcg tggtcatctg gggcggtcag atattcaatc
      601 gtgtagatgc ggccgcaaag gatgtctata gcacaatcgc gaggatggcc gacgatcgcg
      661 ccgaagtcga gaacgcgatc ttgcagtatt atgtcgaaga gatcgatccg gtaaagctcc
      721 aacttgccga aatcgacggt atgatgaacg agcttgatac cttcagcgac tttctcgacg
      781 aacgcgaaca ggaaagccta cgcgaaacca ctgaaggggc attcggcggc ctcggcattc
      841 agatataccc tgtagatcac tatcccacgg taattgcgcc gttggaggga actccggcgt
      901 gggatgtcgg cttgcagtcg ggcgaccaaa tcatcgccat cgaaggtgaa tcgacgcgag
      961 acgaaaagct cgatatcatt gtcggcaagc tgcgtggcgt acccggaacc gaagtaacca
     1021 ttacgatcaa gcggccgggc gttggtgaac cgatcgactt cacgatcacg cgcgcggaaa
     1081 tccatatcaa ttcggtcggg ttctccggcc tcgtttggga tccaaccgca caagatcttc
     1141 cccttgccgc tgcgccggat tggctgcgcg atggcgagga tatcggctac gttcggctga
     1201 aaacgttcgg acgcggcgcg gcggacgaag tccgtgcggc actcgaacag atcaacgccc
     1261 gaaatccgcg cggtgtcgtg ttggatatgc gcatgaatcc gggcggactc ctcgaagagg
     1321 cgcgcgcagt tgccgaccta ttccttccgc gcggtcagat gatcgtgtcg acgaagggtc
     1381 gcagccgctg gagcgaagcg cgtttgtact cggaacgcga gccggttctt ccgacgaatg
     1441 tgcctctcgt cgtgctcgtg gacgagggat cggcgtcagc gtcggaaatt gtcgcgggcg
     1501 cgattcaaga taatgatcgc gggttggtga tcggacggaa cacgtacgga aaaggttcgg
     1561 tgcagac
//
LOCUS       DSCC01000088            1575 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053227, whole genome shotgun sequence.
ACCESSION   DSCC01000088 DSCC01000000
VERSION     DSCC01000088.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053227"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="ENN56_01045"
     CDS             complement(<1..1414)
                     /locus_tag="ENN56_01045"
                     /inference="COORDINATES: protein motif:HMM:PF13204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HDS74102.1"
                     /translation="MVRLRSFGLGCLLLVFTGALAMNETVVPQWQPHDFAFASDREHE
                     NPFAVPFRAIVQTPSSGAFIADGFFDGDAVWKLRVAPWEPGIWSLRTESADPSLDGRE
                     ITFRCAENTDPAIHGGLKIDPEHPYHFIWEDGQRYFLLGYECDWLWALDLTPNGTSPD
                     GIPTVDRFLDRLAANGFNHIILNAYAHDCGWQEGTTGADDYGPPPAFAWGGTNESPDH
                     SRFNLAYWQHYDRIMNALMRRGMVAHVMIKVYNKMVNWPEPGSDLDDRFFRWVIARYA
                     AYPNVVWDFSKESNNEPDLTYKRDRMRFIRKNDPYGRLVTTHDDEREYMNGSYDDVLD
                     FHSDQHHQDWHAETLRKRTAQAWPIVNVELGYEHGPKGLDDFTYGVVQPPEEVARRAW
                     EVCMAGGYATYYYTYTAWDVIRPEDEPPGYAYFRNLAAFFAETNWWRMKPVDELARIT
                     APGERESEIYCLADIGAEYVV"
BASE COUNT          307 a          491 c          446 g          331 t
ORIGIN      
        1 acacaacgta ttccgcaccg atatccgcaa ggcagtagat ctcgctttcc cgttcgcccg
       61 gcgcagtaat gcgcgcgagt tcgtctaccg gtttcatgcg ccaccagttc gtttccgcga
      121 agaacgccgc gagattgcgg aagtatgcgt acccgggagg ctcgtcttcg gggcggatga
      181 cgtcccacgc ggtgtacgtg tagtaatacg tcgcgtaccc gcccgccatg cagacttccc
      241 acgcgcgccg cgcgacctct tccggcggct ggacgacgcc atacgtgaag tcgtcgagcc
      301 ccttcggacc gtgttcgtaa ccgagttcga cgttcacaat cggccacgct tgcgcggttc
      361 gcttgcgcag cgtttcggcg tgccaatctt ggtgatgttg gtcgctgtgg aagtcgagca
      421 cgtcgtcata cgagccgttc atgtactcac gttcgtcgtc gtgcgttgtg acgagtcgac
      481 cgtacggatc gttcttccga ataaaccgca tccggtcgcg tttgtacgta aggtccggct
      541 cgttattcga ttctttcgag aaatcccaga cgacgttcgg atacgccgcg taccgcgcga
      601 tcacccagcg gaaaaaccgg tcgtcgaggt cgctgccggg ctccggccag ttcaccatct
      661 tgttgtacac cttaatcatg acgtgcgcga ccatgccgcg ccgcatgagc gcgttcatga
      721 tccggtcgta atgctgccag tatgcgaggt tgaaacgtga gtgatccggt gattcgttcg
      781 taccgcccca cgcaaacgcc ggcggcggac cgtaatcgtc ggctccggtc gtaccctcct
      841 gccagccgca atcgtgtgcg tacgcattca aaatgatgtg gttgaagccg ttcgccgcga
      901 gtcgatcgag aaaacggtcg actgtcggga taccatccgg cgaggttccg ttcggcgtga
      961 ggtcaagtgc ccagagccag tcgcattcgt agccgaggag gaagtagcgt tgcccatcct
     1021 cccagatgaa gtggtatgga tgctccgggt cgatcttcag gccaccgtga atcgccggat
     1081 cggtgttttc agcacaccgg aaggtaatct cgcgtccgtc gaggctcgga tcggccgatt
     1141 ccgttcgaag tgaccagatc cccggctccc acggcgctac tcgcagcttc cataccgcgt
     1201 ccccgtcgaa gaagccgtcc gcgataaacg cgccgctgct cggtgtctgc actatcgcac
     1261 gaaacggcac agcaaacgga ttctcgtgct cgcggtcgga cgcaaacgcg aaatcgtgtg
     1321 gttgccattg aggaactacc gtctcattca tcgccaacgc tccggtgaat acgagcagca
     1381 gacatccgag cccgaaggaa cgcagacgca ccactccacg gctcccttcg ttccgattcc
     1441 cgctgttcgt atgttttttc gcatgacgga aaccacgaca aaaccaacag tacgtcccga
     1501 gcggcacgaa ccaaactact cgctcggtga gaagatccga tttctcgtcg aaaacggcga
     1561 ttcgcttcgg tacac
//
LOCUS       DSCC01000089            1800 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044451, whole genome shotgun sequence.
ACCESSION   DSCC01000089 DSCC01000000
VERSION     DSCC01000089.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044451"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..131)
                     /locus_tag="ENN56_01050"
     CDS             complement(<1..131)
                     /locus_tag="ENN56_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74103.1"
                     /translation="MDEHVARQIRNIALVGHGGAGKTMLTEALLFTSGAINRMGRIE"
     gene            complement(301..>1800)
                     /gene="recJ"
                     /locus_tag="ENN56_01055"
     CDS             complement(301..>1800)
                     /gene="recJ"
                     /locus_tag="ENN56_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDS74104.1"
                     /translation="VTRLAEAVYKREVVTIFGDYDVDGTSSTALLVSTLGALGVPTEW
                     RIPHRESDGYGLSDRGVELVRETSGTLVITVDCGVTALEQIAALTSDGRDVIVLDHHE
                     TADSLPDAVAVLDPKRRDCPYPFEGLAAVGVAFKLVQALEDVLGIAPGTLMLPALDLV
                     ALGTAADIVPIVDENRVLMRAGLRRLSRKPCVGLAALINVASVNRRNLTTSSIVFGLA
                     PRINAAGRMGSASTAVRLFLATDDAEAFPLAQQLADANRVRQTEDKATLEAAIAEAEE
                     QIGRGVRALVLADASWHPGVVGIVAARLVERYYLPTIMIAMSDPIGRGSGRSIDGFDL
                     HAALGECADVLEGYGGHVKAAGLSITAENVAVFRERFQAVAKRQITDDLLRPKQEIDV
                     VAELGEVSLDTIADIERLGPFGPENRRPVFLSRNVRAAAPPEILKGAHLKVEVYQHGA
                     VREIIGFNMADAAPLFENPVDIAYVPETNTFLGNTRLQLRVKAVRTAEM"
BASE COUNT          386 a          563 c          499 g          352 t
ORIGIN      
        1 ctttcaatac gtcccatacg attgatggca ccggacgtga aaagaagcgc ttcggtgagc
       61 atggtcttgc ccgcaccgcc atgacctacc agagcgatgt ttcgaatctg acgcgcaacg
      121 tgttcatcca tggagaaggc tcccgcacga tagacgtgaa gcgattacgt ggttgagata
      181 tgaaatgaga aagttacgta ctcaccgaaa ggaggtcaac gcatcgaaac cgcacgccaa
      241 ggcatccgat ctacctcgcg aagatccctc aatcggatgc tacacatcgc gaccatcgcg
      301 ttacatctct gccgtacgga cagcttttac gcggagttgc agtcgtgtgt ttcccaaaaa
      361 ggtattcgtc tccggcacgt aggcgatgtc gaccggattt tcgaaaagtg gtgctgcgtc
      421 ggccatattg aatccgatga tctcgcgaac tgcgccgtgt tggtacacct ccaccttgag
      481 gtgagcgcct ttgaggattt ctgggggagc tgccgcgcga acgttccgtg agaggaacac
      541 cggacgacgg ttctccggtc cgaatgggcc gaggcgctct atatcggcaa tcgtgtcgag
      601 cgacacttct ccgagctcgg cgaccacgtc gatctcctgt ttcggacgaa gaagatcatc
      661 ggtaatctgt cgtttcgcca cagcttgaaa acgttcgcga aacaccgcta cgttttccgc
      721 ggtgatgctc agacccgccg ctttcacgtg tcccccgtag ccttcgagca catccgcgca
      781 ctccccaagc gccgcgtgca ggtcgaatcc atcgatactc cgccccgatc cgcgcccaat
      841 cggatcggac atcgcgatca tgatcgtcgg aagatagtac cgttcgacga gccgcgcggc
      901 gacgatcccg acaacaccgg gatgccatga tgcgtctgcg agcacgagcg cgcgaacacc
      961 gcgcccaatt tgctcctcgg cctccgcgat ggctgcttcc aatgtcgcct tgtcctccgt
     1021 ctggcggaca cgattcgcat cggccaactg ctgtgcgagg gggaacgctt cagcgtcatc
     1081 ggtcgcgagg aacagccgaa ccgcagtcga cgcgctgccc atgcgacccg cagcgtttat
     1141 gcgtggcgca agtccaaaca caatactcga cgtcgtaaga tttcgtcggt tcaccgaagc
     1201 gacgttgatc aacgccgcaa gcccgacaca cggttttcgg gatagtcgac gcagccccgc
     1261 ccgcatcagc acgcgattct cgtcgacgat cggaacgata tccgcggcgg taccgagtgc
     1321 gacgaggtca agcgcgggaa gcatgagtgt tcccggtgcg ataccgagta cgtcctcaag
     1381 tgcctgtacg agcttaaacg ccacccccac cgcggcaagt ccctcgaacg gatagggaca
     1441 atcgcgtcgt ttcggatcaa ggaccgcgac ggcatcggga aggctatcag ccgtctcgtg
     1501 gtggtccagc acgatgacgt cgcgaccgtc ggacgtcagt gcagcaatct gttcaagagc
     1561 ggtgactccg caatcaacgg tgattacgag cgtgcctgat gtttcacgca caagttccac
     1621 gccacgatcg ctcaaaccgt acccatcgct ctcacggtgc ggaatcctcc actcagtcgg
     1681 aacgccgagt gcaccgagcg tcgatacgag aagtgccgtc gaactcgtcc cgtcgacatc
     1741 gtagtcgccg aaaatcgtca cgacttcccg cttgtacacg gcttccgcaa gccgtgtcac
//
LOCUS       DSCC01000090            1823 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043720, whole genome shotgun sequence.
ACCESSION   DSCC01000090 DSCC01000000
VERSION     DSCC01000090.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043720"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            129..749
                     /locus_tag="ENN56_01060"
     CDS             129..749
                     /locus_tag="ENN56_01060"
                     /inference="COORDINATES: protein motif:HMM:PF14606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74105.1"
                     /translation="MIGIDDDAFIEPSGHFAISRPIVFYGSSITQGGCASRSGMSYEA
                     ILGRILDVDFVNLGFSGNGLGDPELAEAMAEIDAACYVLDYAVNLKEVDDLRGNYTAF
                     IETIRAKRPDTPIVAITPIFATHERWSAEARSKWNGFRDVIRAEVGLLVSNGDPIRLI
                     EGYSLFGPDIADGMADAVHPNDVGFTAMATRLAPTIADVVGLGIER"
     gene            complement(755..1510)
                     /locus_tag="ENN56_01065"
     CDS             complement(755..1510)
                     /locus_tag="ENN56_01065"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDS74106.1"
                     /translation="MKSLEHITHIMFDVGGTLANDEPSYADGYAAMLTGLGYPSTGAD
                     YRQASDGAKGDVEEAPREIDAFKVWREGYNRAIIRRLGVPEAKIEDVRAAMKKRFRYY
                     SNAYVYPESRFVLRSLRWAGYDVAIISNIQPVLPDLLLDLDLMRYASFAIASDTFGAQ
                     KPDRSIFDEAIRLANVDPKNVIYVGDGIQPDVMGSSQVGMTPVLIDRGGRVTDEQLAE
                     YGGFRVGNLVELLDRLGVDPWNEEWLQDPSRIR"
BASE COUNT          387 a          573 c          519 g          344 t
ORIGIN      
        1 cgcaagcacc cgccccgatc tccgacaaga acgccggcgt cttcgaaggg aaactgtatt
       61 ccggagcgtc gaagcgcatg cgcgacgtga ccttgtaact tccgaactac aacgccgtcg
      121 atgtcgacgt gatcggcatt gacgacgatg cgtttatcga accgtcgggt catttcgcta
      181 tttcgcgacc tatcgttttc tacgggtcat ccatcacaca gggcggatgc gcgagccgtt
      241 ccggcatgtc atacgaagcg atacttggtc gcatactcga tgtggatttc gtgaacctcg
      301 gattcagcgg gaacggactc ggcgatcccg agctcgccga agcgatggcg gaaatcgacg
      361 cggcgtgcta tgtgctcgac tacgcggtga atctcaagga agtcgacgac ctccgcggca
      421 actacaccgc gtttatcgaa acaattcgtg cgaaacgtcc cgacacgcct atcgtcgcga
      481 ttacgccgat cttcgccaca cacgaacgct ggtccgccga agcgcggagc aagtggaacg
      541 gattccgcga cgtcatccgc gccgaggtgg ggctactcgt ctccaacggc gatccgatcc
      601 gactcattga gggatactcg ctgttcggac cggatatcgc cgacgggatg gcggacgcag
      661 tgcatccgaa cgatgtcgga ttcaccgcca tggcgacgcg actcgccccg acaatcgccg
      721 atgtggtggg actcggaatc gagcggtagc cgactcagcg gatacgcgac gggtcctgca
      781 accactcctc gttccatggg tcgacaccga ggcgatcgag gagctcgacg agattgccga
      841 cgcggaaccc gccgtactct gcgagctgct catcggtgac gcggcctcca cggtcgatga
      901 gtaccggtgt catgcccacc tgtgacgacc ccattacgtc cggttggatg ccatcgccga
      961 cgtagatcac gttcttcgga tcgacattcg ccagccggat cgcttcatcg aagatgctgc
     1021 ggtccggttt ctgcgcacca aaggtgtccg acgcaatcgc gaacgacgcg taccgcatga
     1081 gatccaagtc gagcagcaag tcgggcaaca ccggctggat gttcgagatg atcgccacgt
     1141 cataccccgc ccagcgaagc gaacgaagca cgaaccgact ctccgggtag acatacgcgt
     1201 tggagtagta gcggaaccgc ttcttcatcg ccgcgcgaac atcctcgatc ttcgcctccg
     1261 ggacgccgag ccgtcggatg attgcacggt tatacccctc gcgccacacc ttgaatgcat
     1321 cgatctcacg cggcgcctcc tcgacgtcgc ccttcgctcc atcggatgcc tgccggtagt
     1381 cggcgcctgt ggacgggtat cccaagccgg tcagcattgc cgcgtacccg tcggcatacg
     1441 acggctcatc gttcgcaagc gtgccgccga catcgaacat gatatgcgtg atgtgttcaa
     1501 gcgatttcat cgtgcgtcct tctcaatcgt ggtgcctctc tcgatcggca tcctcagccg
     1561 accgactcct tccccgcgta atcgcaacgc cgccatcgac tccagagcgt accgcacccc
     1621 gccgacgcgg tcaacgggag gccgtgagag aaccgtaaaa cgagggcgca atcctctcgt
     1681 cacgtggtgc agtaaaaaaa tactgtggca ccccttgata gattatgatg ggtgttgtat
     1741 attcctgcat gaggaccgga gaagtgccgc aaaaacggaa tgtgcggcaa cggtcaatga
     1801 acacgagaga aacaagaaca acc
//
LOCUS       DSCC01000091            4766 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1011962, whole genome shotgun sequence.
ACCESSION   DSCC01000091 DSCC01000000
VERSION     DSCC01000091.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1011962"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..813)
                     /locus_tag="ENN56_01070"
     CDS             complement(<1..813)
                     /locus_tag="ENN56_01070"
                     /inference="COORDINATES: protein motif:HMM:PF08306.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74107.1"
                     /translation="MKTPLKRPISREHPLLILMVNNDSSYEEEARELVRMWRAFDPFL
                     REHATVQIEGTQSSNWERCETILRYAQPERIPITFQIQGDNGERHDAVPPNRLRDFLD
                     RYDCIVGLQIVEASQRTFVAHGAGPEYSMGRNARYARDAILIAAEYGLFLSWQLMRDN
                     WAAIGCSVDNEALFDAIQEHSEYVIPQHEMNCEFAKQIDHTAAMGMWLSGAVENWGVE
                     AQSWYWSDSGYREPGVCMPGSLDMPGGLYAIMFLLGAAGGATVYSIEPPKDVW"
     gene            complement(842..3109)
                     /locus_tag="ENN56_01075"
     CDS             complement(842..3109)
                     /locus_tag="ENN56_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74108.1"
                     /translation="MKTPLKVKISAEHPLLILMANSPPNAHHMAADYVANAHAMVDHW
                     ASLDDLLREHATIQIEGICDDFWRRVEVLLPVAEENGIPITLQTQTNNADLNDTMPMD
                     RVRRLCDQYTCLVGLQLSEASHRTFVGHGGGPEYSMGRNARYARDIIRLAGEYGLFMS
                     WQLMSENFAAIACSGDNEALFDTIVEYGEYVIPTHEMNSEYAKYIDHLAAMGFWISGA
                     TANWGVEPQSWYWSDAGYAAPGVCIPGSLDMPGELYSIMMLLGATGGATVYSIEPPWD
                     IWSGEHGRHRFTDWIVPTFSRLVSERLIPSRKEVKASMPVAYHLARCERPKDFHVVER
                     DLDFDHGEGLLVRAAYGVYDCARDAEMIPNTSRVGWIPVLPAKTPSAVLNSFARVIRT
                     GELRDEAHAREVLLSHFSDTDRGTAWSSAIGPLVVAANSHENWPVPETVKLEVPAVPE
                     KVRFDGNRLTWDSHEGDQLYYVWRLREGRETCLTPEGVSVNEFIVDEESPKDSGDSYA
                     VSVRTSATESIQATLHLHQFVIFNGRESRRSMWIGKDDSPVSRPRFAENLPDSVDRTI
                     AMEQRAAQCSPVEDLASPLISDDDPHAEAKRGVLAALIAWKQTVESEDIDALERLYDS
                     TYREQDGRTVESLCVAFKSILRKYVMPELGSFWPDYGFLFAWENPVVRLFTREWKDVS
                     DDRIVLDVVFEFWAGGGTELEPSDIFKHPIGGKSKVFRMTWVRRDSEWRIAATDHSMF
                     RMEDTVPFRTRYQGW"
     gene            complement(3201..3761)
                     /locus_tag="ENN56_01080"
     CDS             complement(3201..3761)
                     /locus_tag="ENN56_01080"
                     /inference="COORDINATES: protein motif:HMM:PF08909.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HDS74109.1"
                     /translation="MIQEVDSASTTGEFEIKPIELPPEPDYEIFDTKRIRLFTDESGK
                     CRLTVEADRSYPDVNVLRCFPHSMPNDYWAITDRTNRVVGIIRDPSALDSMSYETAQR
                     MLSIQYFLPVVTAVYSIQEEFGAFYFDIETNLGRRTFVAKGVRDSVDEHEDGEIIITD
                     VDENRYVIRDWASFDAKSRKHLSRIV"
     gene            complement(3810..4748)
                     /locus_tag="ENN56_01085"
     CDS             complement(3810..4748)
                     /locus_tag="ENN56_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS74110.1"
                     /translation="MSLGTLTAFIAYMGMFYQPMHWIMQIGNHINQAITAAQRLFEII
                     DAEKEVYEDPDAVHLDNLHGHVEFDDVRFGYRKEKEVLKGVSFDVAEGQMIGLVGRSG
                     VGKTTLTNLICRFYDVNEGAIRVDGVDLRKVSISSLRRRIGIVPQESFLFNGTIAENI
                     KYAKPDADIDDVIRAASAANALGFILRQPDGFDTRVGGSSGRLSGGEKQRIAIARAIL
                     HDPKILILDEATSSVDTETEDQIQQALRRLVKGRTTFAIAHRLSTLKYADKLVVLDDG
                     KVAEIGSHDELVAKEGVYARLVSMQSKLSAIRAVDG"
BASE COUNT         1090 a         1424 c         1161 g         1091 t
ORIGIN      
        1 ccacacgtct ttgggcggtt cgattgaata caccgtcgct ccaccggctg ctccgaggag
       61 aaacatgatt gcgtagagtc caccgggcat gtcgagcgag ccgggcatac acactcccgg
      121 ttcacggtac cccgaatccg accagtacca tgactgtgcc tcaacgcccc aattttcaac
      181 agcgccgctg agccacatcc ccatcgccgc ggtgtgatcg atctgcttcg cgaattcgca
      241 gttcatctcg tgttgcggaa tcacgtattc ggaatgttcc tgaattgcgt caaataatgc
      301 ttcgttgtcg acagaacacc cgattgccgc ccaattgtct cgcatgagct gccaactgag
      361 aaacagtccg tattcggcgg cgatgagaat cgcgtcccgt gcgtaccgag cattccgccc
      421 catgctgtat tccggcccgg cgccatgtgc aacgaacgtc cgttgactcg cttcaacgat
      481 ctgcaagccg acgatgcaat cgtatcgatc gagaaaatcg cgaagacgat tcggaggcac
      541 cgcgtcgtgg cgctcgccgt tgtcaccttg tatctgaaac gtgattggaa tccgttccgg
      601 ttgagcgtaa cggagaattg tctcgcatcg ctcccagttc gacgactgcg taccttcgat
      661 ctgcacggtc gcatgctctc gaagaaaagg atcaaacgca cgccacatac gtacgagttc
      721 gcgtgcttcc tcttcgtacg atgaatcgtt gttgaccatg aggatgagaa gcggatgttc
      781 gcgactgatc gggcgcttca atggcgtttt cattttgaac ctcttcgagg tcaacgtacg
      841 tttaccaacc ttgataccgc gtcctgaagg gcacagtgtc ctccatacgg aacatcgaat
      901 gatccgttgc agcaatacgc cactcggagt cgcgtcgaac ccacgtcatc cgaaacacct
      961 ttgatttgcc accgatcggg tgcttgaaaa tatcactcgg ttcaagctcc gtcccgccac
     1021 ccgcccagaa ttcaaataca acgtcgagca caattcggtc gtccgagacg tctttccact
     1081 cgcgcgtaaa cagacgaacc accgggttct cccacgcaaa aaggaatccg taatcgggcc
     1141 agaatgatcc aagttcgggc atcacgtatt tgcgaagaat cgatttgaac gcaacacaaa
     1201 gcgactcgac ggtgcgcccg tcctgttctc gataggtcga atcgtataac cgctccaacg
     1261 catcgatatc ttccgactca accgtctgct tccacgcaat tagagccgcc aggactcctc
     1321 gctttgcctc tgcgtgcgga tcgtcgtcag agatgagcgg cgacgccaag tcctcaaccg
     1381 gcgagcactg cgccgcccgc tgctccattg cgatcgttcg gtcgaccgaa tcaggcaggt
     1441 tctcggcgaa gcgtgggcga gatacgggag aatcgtcttt gccaatccac atcgaccgac
     1501 gactctcacg accgttgaag atcacgaatt gatgaaggtg taacgtcgct tggatcgact
     1561 cggtggcaga agtcctcacc gataccgcgt aagaatcccc tgaatccttg ggggattctt
     1621 cgtctacgat gaactcgttg acggaaactc cctccggtgt aagacacgtc tctctgcctt
     1681 cacgcagccg ccacacgtag taaagctggt cgccttcgtg tgagtcccat gtcaatcgat
     1741 tcccatcaaa ccggaccttt tccggtaccg cgggaacttc gagcttcact gtctccggca
     1801 ccggccagtt ctcgtgcgaa ttcgccgcga caacaagcgg accgatcgca gagctccatg
     1861 cggtgccacg gtccgtatcc gagaagtgag ataacagcac ctcacgcgca tgcgcttcat
     1921 cacggagctc cccggtacgg ataacacgtg cgaatgaatt caacactgcg gaaggcgttt
     1981 tcgccggcag aaccggtatc caaccaactc gcgacgtgtt cggaatcatc tcagcatctc
     2041 gcgcacaatc atacacacca tacgcggcgc gaaccagtag cccttcgccg tgatcgaaat
     2101 cgagatcacg ttcgactaca tggaaatcct ttggtcgttc acaacgcgca aggtgatatg
     2161 ctaccggcat cgatgctttg acctctttac gcgatgggat aagccgctcc gataccagtc
     2221 gcgaaaacgt tggaacaatc cagtcggtaa agcgatgtcg accatgttcg ccggaccaga
     2281 tatcccacgg tggttcgatc gaatacacgg ttgcgccgcc ggttgcaccg agcagcatca
     2341 tgatcgaata caattctccc ggcatatcca gactacccgg aatacacaca cccggagccg
     2401 catatcccgc gtccgaccag taccacgact gcggttcgac accccaattc gccgtggcac
     2461 cactgatcca gaatcccatc gccgcgagat ggtcgatata cttggcatat tcgctattca
     2521 tctcgtgcgt cggaatgaca tactcaccgt actctacaat ggtatcgaag agcgcttcgt
     2581 tgtcccccga acacgcgatt gctgcgaagt tttcgctcat caactgccaa ctcatgaaca
     2641 acccgtactc gccggcaagt cgaatgatat ctcgcgcata ccgcgcgttt cttcccatgc
     2701 tgtattccgg accaccgccg tgcccgacga atgtccgatg gctcgcctca ctcagttgca
     2761 gtccgacaag acacgtatat tggtcgcaga gtcgacgaac gcggtccatc ggcatggtat
     2821 cattgagatc ggcattgttc gtttgcgtct gcaacgtgat agggatacca ttctcctctg
     2881 cgacaggaag caacacctca acgcgccgcc aaaaatcgtc gcatatccct tcgatttgga
     2941 tcgttgcgtg ttcccgcagc agatcgtcca atgatgccca atgatcaacc atggcgtgtg
     3001 cgtttgctac gtagtcagcg gccatatgat gagcattcgg cggtgaattc gccatgagaa
     3061 tcagaagcgg gtgttcggcg gagatcttga ctttcaacgg tgtcttcaca gctcactcca
     3121 ataagccgat gatcgtacaa aaaagacgtc ggacgattga gaatcgtccg acctcgtacc
     3181 ggacgcaagt gccgaagtga ttacacgatt cgagaaagat gctttcgtga tttcgcgtcg
     3241 aaactcgccc aatctcggat aacataccgg ttctcgtcga catcggtaat aatgatctcg
     3301 ccgtcttcgt gttcgtcgac actgtcacga acgcctttcg caacgaacgt tcgtcgcccg
     3361 agattcgtct cgatatcgaa ataaaaagcg ccgaactctt cctgtatgga gtacactgcg
     3421 gtaacgacgg gaagaaagta ttggatactc agcatccgct gtgcggtttc atacgacatc
     3481 gaatcaagcg cggatgggtc ccgaatgatc ccgacaacgc gattcgtccg gtcggttatc
     3541 gcccaataat cgttcggcat ggaatgcgga aagcatcgca atacattcac gtccggatac
     3601 gatcgatcag cttcgaccgt taaacgacat ttccccgatt cgtccgtaaa aagacgtatc
     3661 cgtttcgtat cgaaaatctc atagtccggc tccggcggta attcgatcgg ctttatctcg
     3721 aattcacccg tcgtactggc cgagtccact tcttgtatca tctcgatctg tcctcatccg
     3781 tctctaagcg gcgttcattg actcgttcgt caaccatcta ccgcgcgaat cgcactgagc
     3841 tttgattgca tcgatacgag ccgtgcgtat acgccttctt ttgccacaag ttcatcgtgt
     3901 gagccgatct ccgccacttt cccgtcatcg agaaccacaa gcttatcggc gtacttcaac
     3961 gtgctgagac ggtgcgcgat ggcgaacgtt gtacgtccct tgacgagtcg tcgcagcgct
     4021 tgttgaatct ggtcctcggt ttcggtgtca actgacgatg tcgcttcatc gagaatgaga
     4081 atcttcggat cgtggaggat cgcacgtgcg atcgcaatcc gctgcttttc accgcctgac
     4141 aagcgtccgc tacttccacc gacgcgggta tcgaacccgt ccggttgtcg caaaatgaac
     4201 ccgagtgcat ttgctgcact tgccgcacga atcacgtcat cgatatcagc atccggcttc
     4261 gcgtacttga tattctcagc gatcgtcccg ttgaaaagga acgactcctg cggtacaata
     4321 ccgattcgac gccgcaggct gctgatactc accttccgga gatcaactcc gtcaacacga
     4381 attgcacctt cattcacgtc gtagaaccga caaatcaggt tggtgagtgt cgtttttcct
     4441 acgccggagc gaccgacaag gccaatcatt tgtccctcgg ccacgtcaaa cgatacccct
     4501 ttgagcactt ccttctcttt acggtacccg aagcgaacgt cgtcgaactc gacgtgcccg
     4561 tgcagattgt caagatgcac cgcgtcagga tcttcgtaca cttccttttc tgcatcgatg
     4621 atctcgaaca agcgttgcgc agccgtaatc gcttgattga tatggttgcc gatctgcata
     4681 atccaatgca tcggctgata aaacattccc atgtaagcga taaacgcagt aagggtgccg
     4741 agcgagatct gctcatgccc cagcac
//
LOCUS       DSCC01000092            2446 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029325, whole genome shotgun sequence.
ACCESSION   DSCC01000092 DSCC01000000
VERSION     DSCC01000092.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029325"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            33..1073
                     /locus_tag="ENN56_01090"
     CDS             33..1073
                     /locus_tag="ENN56_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74111.1"
                     /translation="MPHSFREEIFALGMGGAYTAGAWRGSGLFYNPASLAYKRFHLNV
                     PLRLEVGGIGGITQAQNVVSFYNDNEEKLRNLGSLPPSQVVILDDQMKELDGTGGVAR
                     VFPAVRLGWRNFGFQTYAIFNGAPQMDSGVFQPRIDLNGFTDLGAIAGYSRPFFYRTR
                     RLDLRWYGGVALKVFGRWGINHGFTLSEATRGYEFYDELADAVDDRPEYGVGADIGMM
                     IPLTHDRSASIGIVVQDAITYGQIRPEMSINVGAYWRVLSRIHLVADYRDVLNTADTK
                     WPMHVHFGGEFDLTMLRLRAGAYQGYPTLGLGVNLWLLKVDIVYYSQERGERLGHLTE
                     TNVALELQVGLD"
     gene            1175..>2446
                     /locus_tag="ENN56_01095"
     CDS             1175..>2446
                     /locus_tag="ENN56_01095"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00672.23,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS74112.1"
                     /translation="MHPNHPRTIAALVLAVTVLAAFALGIIELVSVRLMGDYHTIAST
                     IAVAVIIIALYVLARRFVSRPITNVIDTMKRFATGETTARTTADKHGYGEVGLLAAEF
                     NAMADRLQQARAALEISEMRMRRIVESTGEGILLTSLSGTIISANETFCRMFGFDVTD
                     LIGKRVLELIREANRNTLRTLFENARPDITTKEELQCHRVDGTPIDVTIGIVIVEVDG
                     EKFALIMFDDVTAERRATRALRDSEEQYRGLVESISDLVFSLNEEAICTYVNPQITYL
                     LGVSPNDVVGRTLARYIHPDDIETVTAYFREVFAGTADDAPIEFRIVTNNGAYRIVRT
                     RSRIVSHTDGSRSMVGIATDITEYQTTESALRASEEKYRKVFENVQDVFYQTDLAGTI
                     IEISPSIERYSGYPREEVIGNSVAIFYYNPED"
BASE COUNT          555 a          690 c          664 g          537 t
ORIGIN      
        1 cgatacctac gagcgtgcat gcggaacgaa acgtgcctca cagcttccgc gaggagatct
       61 tcgcgctcgg tatgggcggc gcatataccg caggagcatg gcgcggatcg gggttgttct
      121 acaatcccgc gtcactggcg tacaagcgat tccacctcaa cgtaccgctc cggcttgaag
      181 tcggaggtat cggtggcatc acgcaggcgc agaacgtcgt ttcattttac aacgacaacg
      241 aagagaaact gcgtaacctc ggcagtcttc ctccgtcaca ggtagtgata ctcgatgacc
      301 aaatgaagga gctcgacgga actggcggcg ttgcacgtgt cttccccgca gtccgtctcg
      361 gatggcgtaa tttcggattc cagacgtacg cgatcttcaa tggcgcgccc cagatggatt
      421 cgggtgtatt tcaaccacga atcgacttga atggctttac cgatctcggc gcgatcgcgg
      481 gatactcacg accgttcttt tatcgaacgc ggcgactcga cctgcgatgg tacggcggtg
      541 tggcattgaa ggtttttggc agatggggca tcaatcacgg attcacactt tccgaggcaa
      601 ctcgcggata cgagttctac gacgaacttg cggacgcggt cgacgataga ccggaatacg
      661 gcgtcggtgc ggatatcgga atgatgatcc cattgaccca cgaccgcagc gcgagcatcg
      721 gtatcgttgt gcaggacgct atcacctatg gtcagatacg gcccgaaatg tcaatcaacg
      781 tgggcgcgta ctggcgggtt ctttcacgca tacaccttgt cgccgactac cgcgacgtgc
      841 tgaatacggc ggacacgaaa tggccgatgc acgtccactt cggcggtgaa tttgacctca
      901 ccatgttgcg actccgtgcc ggtgcgtatc aggggtatcc gacactcggc ctcggagtga
      961 atctttggct gctcaaggtc gatatcgtct actattctca ggaacgtggc gaacgactgg
     1021 gtcacctcac cgaaacgaac gttgcgctcg agctccaagt cggcctcgac tgacttctga
     1081 aactctatca cgtgttccgt atgcgctttg tatattgtag attgaacggc catatcccga
     1141 aaccatcact catacgcagc atttggatac ccatatgcac ccaaatcacc cacgcacgat
     1201 cgcggcactg gtcctcgctg taaccgtcct cgcggccttc gcactcggta tcatcgagct
     1261 cgtatcggtt cggctgatgg gcgattacca taccatcgcg tcaacaatcg ccgttgccgt
     1321 gataatcatc gcgctctacg tactcgcccg ccgatttgtt tcgcgtccga ttaccaacgt
     1381 tatcgacacc atgaagcgat tcgccacagg cgaaacgacg gctcggacca ctgccgacaa
     1441 acacggatat ggcgaagtcg gtctccttgc cgcggagttc aacgcgatgg cggatcggct
     1501 tcagcaagcg agagccgcgt tggaaatctc cgagatgcgc atgcgtcgca tcgtagaatc
     1561 caccggcgaa ggcattctgc tgacaagcct cagtggcacg attatcagcg cgaacgagac
     1621 gttttgccga atgtttggat tcgacgtcac ggacctgatt ggcaaacgtg ttctcgaact
     1681 gattcgtgaa gcgaatcgga acacgcttcg aacgcttttc gagaatgcgc gaccggacat
     1741 tactacaaaa gaagaattgc aatgccatcg cgttgacgga actccgatcg atgttacgat
     1801 cgggatcgtc atcgtcgaag ttgatggcga gaagttcgcc ctcatcatgt ttgatgacgt
     1861 cacggcggaa cgcagagcta cgcgtgcgct tcgagattcc gaggagcagt accgcggtct
     1921 cgtcgagagt atcagcgacc tcgttttttc gctgaacgaa gaggcaatct gcacctatgt
     1981 gaatccacag attacctatc tacttggcgt ctcacctaac gacgttgtgg gccgcacctt
     2041 agcgcgatac atccatcccg acgacatcga gaccgttacc gcatacttcc gtgaggtatt
     2101 cgccggtact gcggacgatg ctccgatcga gttccgcatc gtcacgaaca atggcgcgta
     2161 tcggattgtt cggacacgtt cgcgcattgt tagtcacacc gatggaagcc gctcgatggt
     2221 cggtatcgct acggatatca ctgaatacca aacgaccgaa tccgcactgc gcgccagcga
     2281 ggaaaagtat cggaaggtat tcgaaaacgt tcaggatgtg ttctatcaga ccgatctcgc
     2341 cggcacgatc atcgagatca gcccatcaat cgagcggtat tcagggtatc cgcgtgagga
     2401 agtcatcgga aactccgtgg cgatcttcta ctacaacccg gaagac
//
LOCUS       DSCC01000093            1588 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052712, whole genome shotgun sequence.
ACCESSION   DSCC01000093 DSCC01000000
VERSION     DSCC01000093.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052712"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..516
                     /locus_tag="ENN56_01100"
     CDS             <1..516
                     /locus_tag="ENN56_01100"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS74113.1"
                     /translation="DPDFIPAVVLITTLGGPLSLTLGSDETTRSSRLYRELVEGELAV
                     YVNCSMEATIEPFVIDIAAMVWQDRTHEEVESAIDRVIASAVSHPVTADELARAKKQL
                     IATLVYENERISGRALMLTLAHLVFGTELLRTYADRIAAVTADDVRRVAARLFDPRNR
                     VVGRYVPNDES"
     gene            559..>1588
                     /locus_tag="ENN56_01105"
     CDS             559..>1588
                     /locus_tag="ENN56_01105"
                     /inference="COORDINATES: protein motif:HMM:PF01738.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDS74114.1"
                     /translation="MELPIAFENEGQQLIGVLHVPNGASKVPGVVMCHGFTGNRSESH
                     FLFTKAARAFAEAGIAVLRFDFRGSGDSDGSFEDMTISSEISDAVRAIEVLAQRPEVD
                     ANRIGIVGLSLGGCVAACATARSERVRATVLWAPVHDPKVIAYHRNATEQVFPWEFTG
                     GLLLGEALVRELPTIDPVAELRNVRGPVLILHGSADQTLPVEGSEAFVRAIESAGGQV
                     EREIVDGADHTFASVNHERYAIDRTASWFRDVFAAIEPEIAPSAKRISYADAGVDIEA
                     ANVTKKRLRELVRESYTPNVLTELGSFGGLFLANFPDLEEPVLVSSTDGVGTKLKVAI
                     AANRHDTVG"
BASE COUNT          369 a          423 c          477 g          319 t
ORIGIN      
        1 gacccagact tcataccggc tgttgtattg attacgacac tgggaggacc gctcagcctt
       61 acgttgggct cggatgaaac gactcgaagc tcccgactat acagagagct cgtagaaggt
      121 gagctcgcgg tatacgtcaa ctgctccatg gaggcgacga tcgagccatt cgtcatcgac
      181 attgcggcta tggtatggca ggaccgaacg cacgaggagg ttgaatcggc gatcgaccgc
      241 gtaatcgcaa gtgccgtatc gcatccggtt actgcggatg aactcgcccg tgcaaagaag
      301 caacttatcg ccacactcgt gtacgaaaac gaacgtatct ccggacgcgc gttgatgttg
      361 acgctggcgc acctcgtgtt cgggacagaa ttgctccgca cgtacgctga ccgtatcgcg
      421 gcagtgactg cggatgacgt gcgccgggtt gcagcccggt tatttgaccc gagaaatcgc
      481 gtagtcggcc gatatgtacc gaacgacgaa tcatagaatc gccgaaaaat ccatcgcact
      541 taaactacgg aatcgataat ggaactacca atcgcatttg aaaacgaagg gcagcaactc
      601 attggtgtgc tccatgtacc caacggtgca tcgaaggtgc ccggagtcgt aatgtgtcac
      661 ggtttcacgg gaaaccgttc ggaaagccac tttctcttta ccaaggccgc ccgtgcgttt
      721 gcagaggccg gcatcgccgt attgcgattt gacttccgag gatcgggaga tagcgatggc
      781 tcgttcgaag acatgacgat ctcaagcgag atttccgatg cagtacgagc gatagaggta
      841 ctcgcgcaac gaccggaagt cgacgcgaat cgcatcggta tcgtcggttt gagcctcggc
      901 ggctgcgttg ccgcgtgtgc gaccgctcgc tcggagcgag tgagagcgac ggttctctgg
      961 gcaccggtac acgacccaaa agtcatcgcg taccaccgaa acgccacgga gcaagtattt
     1021 ccttgggaat ttacgggggg attactgctc ggcgaagcgc tcgtgcgcga actgccaacc
     1081 atcgatccgg tcgcagagtt acggaatgtg cgcggacctg tgctgattct ccacgggtcg
     1141 gcggaccaga cgcttcctgt tgaaggaagc gaagcgtttg tccgcgcaat tgaatcggcc
     1201 ggtggtcaag tcgagcgaga aatcgtcgac ggtgcggacc atacgtttgc ttcggttaac
     1261 catgaacggt acgcgatcga ccgaacggca tcatggttcc gcgacgtatt cgcagcgata
     1321 gaaccggaga tcgcgccttc agcaaagcga atcagctacg cggacgccgg cgtcgatatc
     1381 gaagcggcaa acgttacgaa gaaacggcta cgcgagctgg tacgcgagag ctatacaccg
     1441 aacgtcctga cggaactcgg atcatttggc gggctgttcc ttgcgaactt tccggatttg
     1501 gaagaaccgg ttcttgtctc gagtacggac ggagtcggga caaagttgaa agtggcgatc
     1561 gcggcgaacc ggcatgatac cgtcggta
//
LOCUS       DSCC01000094            2171 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034432, whole genome shotgun sequence.
ACCESSION   DSCC01000094 DSCC01000000
VERSION     DSCC01000094.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034432"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1471
                     /locus_tag="ENN56_01110"
     CDS             <1..1471
                     /locus_tag="ENN56_01110"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF00672.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDS74115.1"
                     /translation="GSTEEILRPVGVTILPDASQAQQPEFHVAYALCSTVPWGVILQE
                     PVVKSIWTVSHILRDISLWLAIALTIAILGGLLLSRMIGRPILRLTQAVKEIGAGNFD
                     HRVPFDRGDEFGELAHGVNSMAQRLKVFDDINVGKVLREKSKLEAIVHNIADGVIFTG
                     SNGEVLAMNEPVERWFGVRESECLNRVVAECVEEKELADLINETANDLGKTLHTHELS
                     LEIPGTVHETRLIARAMRVQSDTGETVGVTTILRDVTVEREVDRMKTELLSIVAHELR
                     SPLVSIMGFSGILLEKELDLATRLEFAETVNREANRMVEMLNKFLNISRIESGKTEFT
                     KTPADVTEIVRDVVRINSGIADEKTIQIELHAPNRVTQVLVDRELIGQAVLNVLTNAV
                     KYSPVNTTVRITVTEHRNTIEVAVKDEGFGITSENQKRLFEKFFRVTDDPNVRDITGT
                     GLGLPLVKEIVEQHGGRVHVESTLGAGSTVSFHLPKNLV"
     gene            1579..>2171
                     /locus_tag="ENN56_01115"
     CDS             1579..>2171
                     /locus_tag="ENN56_01115"
                     /inference="COORDINATES: protein motif:HMM:PF01578.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDS74116.1"
                     /translation="MVHTLELLAVLLPLLYALLTAAYAIVFFRDEPGVARLLTPSLSV
                     VLILHLLYVALRTALYEHIPLANVFETLTVMGFALTFVYWITERFVKTSNTGMFVLGV
                     VFLFQTISSAFIGHEVEVNPVLRSPLFGAHTTTAVLGYTGFALSAVYGVLYLMLYHEL
                     KVNRFGIIYGRLPSLGTLGVMHERAAFVGLIAMTAAIT"
BASE COUNT          509 a          569 c          584 g          509 t
ORIGIN      
        1 aggtagtact gaagaaatac tccggccggt aggtgtgacg attctccccg acgcctccca
       61 agcgcaacag ccggagtttc atgtggcgta cgctctgtgt agcacggttc cgtggggcgt
      121 gattcttcaa gaaccggtcg ttaaatcgat ctggacagtt tcgcatatcc tccgcgatat
      181 atctctctgg cttgccattg cgttgaccat tgcaatcctc ggtggtctac tcctttcgcg
      241 gatgattggg cgtcccatat tgcgcctcac acaggcagtc aaggagatcg gtgccgggaa
      301 tttcgatcac agagttccgt tcgatcgggg agacgagttt ggcgagctcg cgcatggcgt
      361 caacagcatg gcacagcgcc tgaaagtatt cgacgatatc aatgtcggga aggttctccg
      421 cgagaagtcg aaacttgaag cgatcgtaca taacattgcc gacggggtca tcttcacggg
      481 gtcgaacggc gaagtcttgg cgatgaacga gccagtcgaa cggtggtttg gcgtccgcga
      541 gtctgagtgc ttgaaccggg tcgtcgccga atgcgtagaa gagaaagaac tcgcggatct
      601 gatcaacgag accgcgaatg acctcggaaa aacacttcac acacacgagc tttcgcttga
      661 gataccggga actgtccatg agacaagatt gatcgcccgt gcgatgcgtg ttcaatccga
      721 taccggtgaa acggtcggcg ttaccacaat tctacgcgac gtaacggtcg aacgtgaagt
      781 cgaccggatg aagacagagc tattgtcaat cgtcgctcac gaattgcggt cgcctctcgt
      841 atcgataatg gggttcagtg gtattctgct cgaaaaggag cttgatctcg cgactcgact
      901 cgaattcgct gagacggtga acagagaagc gaatcgtatg gtcgagatgc tcaacaagtt
      961 cctgaatatc tcgcgaattg aatcggggaa gacggaattt accaaaacgc ccgccgatgt
     1021 aacggaaatc gttcgcgatg tcgtcaggat taacagtggc atagccgatg aaaagaccat
     1081 tcagatagag cttcatgcac caaaccgagt aactcaggtt ctcgtggatc gtgaactgat
     1141 cgggcaggcg gttctcaacg tgctcacaaa cgcagtgaaa tacagtcctg taaacacaac
     1201 agtccgaatc acggtgacag agcatcgtaa tacgatcgaa gtcgccgtaa aggacgaagg
     1261 atttgggatc acgtcggaga accaaaaacg cctcttcgaa aagttcttcc gcgtaaccga
     1321 tgatccgaac gttcgcgata tcaccgggac gggacttggt cttccattag tcaaggaaat
     1381 cgtcgaacaa cacggagggc gggttcacgt agaaagtaca cttggtgccg gctcgacggt
     1441 gtcgttccac ctaccgaaga acttagtcta aggtcccatt aggccgcgaa tacgggcgct
     1501 tctgcttgac ccgtcacgaa gcaccccgta ccattcccgt tcgaattgac tgatcagtat
     1561 actttctggg acatcctcgt ggttcataca cttgaattgc tggcggttct cctaccgttg
     1621 ttgtacgcgc tgttgaccgc cgcgtacgcc attgtgttct tccgagatga acccggcgtc
     1681 gcacgcctgc ttacgccgag ccttagcgtc gttctcatcc ttcacctcct ttacgtcgcg
     1741 ttacgcacgg cgctctacga acatattccg ttggcgaatg tattcgagac actgacggtg
     1801 atgggttttg cactcacgtt tgtgtactgg atcaccgagc gattcgtaaa gacgtcgaat
     1861 acgggaatgt ttgtactcgg tgtcgtgttt ctcttccaga caatttcttc ggcgttcatc
     1921 gggcacgaag tggaagtgaa cccagtgctt cgcagtccac ttttcggtgc gcacaccaca
     1981 accgcggttc ttgggtacac cgggttcgcg ctttcagcag tctacggcgt actctatctc
     2041 atgttgtacc atgagttgaa agtgaaccgc ttcggaatca tctacggtcg actcccatca
     2101 cttggaaccc tcggagtgat gcacgaacgc gcagcctttg tcggactcat cgcaatgacc
     2161 gcggctatca c
//
LOCUS       DSCC01000095            1788 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044846, whole genome shotgun sequence.
ACCESSION   DSCC01000095 DSCC01000000
VERSION     DSCC01000095.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044846"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1788)
                     /locus_tag="ENN56_01120"
     CDS             complement(<1..>1788)
                     /locus_tag="ENN56_01120"
                     /inference="COORDINATES: protein motif:HMM:PF13361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74117.1"
                     /translation="LKTNFRSYPAIIDAVNRVFGAPGVFRDEGTRDATSSYHPAYAPL
                     EPPPDSPDDVGDTPRRILRVTDGRDGEKVNADERRAREATVIAATIRQAVDEGWPVRD
                     PITAAVRPLQYGDVAILYRTTTGIALLETALQNASVPYYQIGGKDFYRREEIARLVSV
                     LSAIEQPNDALSVVAALRSPFFGVSDTDLLRTKPNFDYRNAPLELPGTVRHAYEILRE
                     LHAMRRESHPSALLQRLYERTKVLGVFALSKNGDQAIANLLKILDDARRVEAIEPVSF
                     RRFNDRLRRMREDGVTEGEAEFTAHDDTGSGRVHLLTIHKAKGLEFPVVCVFDLAAGK
                     GRGTSSPVDLHTIRDADHERPRVGLQISGDSIHLSAPGFLQFADENAAREDAESKRLL
                     YVALTRARDYLLLPTIVSKTSSNWERIIDDALPANAQTAFTPHEAPPTGDTSSARIDT
                     TTEELDAIRSAFETARRELYERAVKPRVQFRRPSMHGDERASHKDVESDEARTRDEAQ
                     RLGTVVHAAMEHVVMKRDACTDDQISTLIDELADEHGLYSTGLRNDAAALTRRGLRVD
                     VVRRARAAKRAWTEVPVMLCEPDVSGDGIS"
BASE COUNT          323 a          550 c          525 g          390 t
ORIGIN      
        1 gagattccgt cgcctgaaac gtccggttcg cagagcatta ccggtacttc cgtccatgcg
       61 cgtttcgcgg cgcgtgcgcg acgcaccaca tccaccctca gtccgcgtcg agtgagtgcc
      121 gccgcgtcgt tgcgaaggcc ggtcgaatac aatccgtgct cgtccgcgag ctcatcgata
      181 agcgtggaga tttggtcatc cgtacacgcg tcgcgcttca tcaccacgtg ctccattgcc
      241 gcatggacta ccgtgcccag acgttgcgct tcgtctcgcg tgcgcgcttc gtcggactcg
      301 acgtccttgt gactcgctcg ctcgtcaccg tgcatagacg gtcgacggaa ttggaccctc
      361 ggcttgaccg cgcgttcata cagttctctc cgcgcggttt cgaacgctga acgaatcgcg
      421 tcgagctcct cggtcgtcgt atcaattctc gcggaagacg tgtcgccggt cggaggagct
      481 tcgtgcggag taaatgccgt ctgcgcgttg gccggcagtg cgtcgtcgat aattcgctcc
      541 cagttactgg atgttttcga tacgatcgtc ggcagtaaga ggtagtcccg tgcgcgtgtc
      601 agagcgacat agaggagccg tttcgattcg gcgtcctcgc gtgcggcgtt ctcgtcggcg
      661 aattgtagaa accccggtgc gctcaggtgt atcgaatcgc ccgatatctg caatccgacg
      721 cgtggacgtt catggtcggc gtctcgaatc gtgtgcaggt ctaccggcga actcgttccg
      781 cgcccctttc ccgctgcgag atcgaacacg caaacaacgg ggaattcaag ccctttcgcc
      841 ttgtgtatcg taagcagatg cacgcgtcca ctgcccgtgt cgtcatgagc ggtaaactcc
      901 gcttctcctt cggtaacacc gtcctcccgc atcctccgta ggcgatcatt gaaccgtcgg
      961 aacgaaacag gctcaatcgc ctcaactcgt cgcgcgtcat cgagaatctt cagcagattc
     1021 gcaatcgcct gatccccatt ctttgaaagc gcgaagacgc caagcacctt cgtcctctca
     1081 tacagacgtt ggaggagtgc cgacgggtga ctttcccgac gcatcgcgtg aagctcacgg
     1141 agtatctcgt acgcgtgccg tacggtaccg ggaagctcta acggagcgtt gcggtaatcg
     1201 aagttcggct tcgttcggag gaggtcggtg tcagataccc cgaagaacgg cgagcgcagc
     1261 gccgcaacga ccgacagtgc atcgttcggc tgctcgatgg cactgaggac gctgacgagc
     1321 cgcgcgattt cctcccgtcg gtagaaatcc tttccgccga tctggtagta cggcaccgac
     1381 gcgttctgga gcgcggtctc caatagtgcg ataccggttg ttgtgcggta gagaatcgcg
     1441 acgtcaccat actgaagcgg acgaaccgca gccgttatcg gatcacgaac cggccagcct
     1501 tcgtcgaccg cttgccgtat tgtcgccgcg attaccgtgg cttcgcgcgc acgtcgttcg
     1561 tctgcgttca ctttttcgcc gtcacgaccg tcggtcacgc gcagtatgcg acgtggagta
     1621 tctcccacgt catcggggga atccggtggt ggctccagag gagcgtacgc tggatggtac
     1681 gacgatgttg catcgcgtgt gccttcgtcg cggaaaaccc cgggagcgcc gaacacacgg
     1741 ttcaccgcgt cgatgatcgc gggatacgaa cggaagttcg ttttcaac
//
LOCUS       DSCC01000096            2523 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028135, whole genome shotgun sequence.
ACCESSION   DSCC01000096 DSCC01000000
VERSION     DSCC01000096.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028135"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            99..2102
                     /locus_tag="ENN56_01125"
     CDS             99..2102
                     /locus_tag="ENN56_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter KefB"
                     /protein_id="HDS74118.1"
                     /translation="MHIPILTDLVVIFALSIPVLYICHRLNIPTIVAFLLTGMLVGSS
                     GFGLVHAVDEVNILAEIGVVLLLFTIGIEFSFENLMRIKRTVLIAGALQVVFTMAATA
                     AIAMAFGLPPRMAVFAGMLVSLSSTAIVLKILQERGQIDAPIGGTSLGVLIFQDIAVV
                     PMMIVTPILAGSESLTFQSLSVLVLKILAVIIIVIVAARWLVPHGLYRIARTRDNDLF
                     LLAVVFLAFATAWLTAQVGLSLALGAFLAGLIISESEYSERALGSVLSFRDVFTSFFF
                     ISIGLLMDVRILWQSPVLVFGFTVGVILLKIVTTVSAVAILGLPFRTAALTGVALSQM
                     GEFSFILSKPGIETGLLTPEHYQIFLAVSILTMIAAPFMIMHAPAIAGLIERLPIPDR
                     VRRGITKQPPEAPESDLSEHLIVVGYGLTGRNVVRAAIEALIPYEIIELNPDTVHAER
                     QNGEVIHYGDASQPNVLLYAGIKRAKVFVITIPDAVSTRRIVEVARRMNPDLHIIART
                     RYVREMETLYALGANEVIPEEYETSVELFSRVMHSYLVPYDVIDRFIAEVRADSYEML
                     RDAPATAPIHIVRHLQGLDITTFQVANGASVVGQTLAEIGLRKNHKVTALAIHRAGEK
                     INVPDGTAEFHAGDLVVVAGCPDRLAEVAPLFRAPADASSETE"
     gene            2124..>2523
                     /locus_tag="ENN56_01130"
     CDS             2124..>2523
                     /locus_tag="ENN56_01130"
                     /inference="COORDINATES: protein motif:HMM:PF02837.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDS74119.1"
                     /translation="MTASYSLDGEWTLRGFPERSHTVDSPDMLDDLPVEPISAQVPGN
                     VELDLVRAGVIDYDPYVGDNILRLKEYEGWEWWYRRSFDGPTETGKRLTLRFDGLDCI
                     ADIWLNAMHLGRRENMLIPQEFDVTDIISRG"
BASE COUNT          497 a          740 c          699 g          587 t
ORIGIN      
        1 attgtcgaga cacggcccac ggtgtaccgt ttttaacaca gccacccacg catacttcgt
       61 agtgaacctg cgacccgact cattgaaagt gatcgtgtat gcacatcccg attctcactg
      121 atcttgtcgt aatctttgcg ctctcgatac cggttctcta tatctgtcac cggctgaata
      181 tccctacgat tgtcgccttt cttctcaccg gtatgctcgt aggttcgagc ggcttcgggc
      241 tcgtacacgc cgtggatgag gttaacatcc tcgccgagat tggtgtcgta ctgctcctgt
      301 tcactattgg tatcgagttc tcattcgaga atctgatgcg gatcaagcgc acggtactta
      361 tcgcgggagc gcttcaggtc gtattcacca tggcggcgac ggcggcaatc gctatggcgt
      421 ttgggctgcc accgcgtatg gctgtgttcg cggggatgct tgtatccctg agtagtaccg
      481 cgatcgtgtt gaaaatcctt caggaacgcg gacagatcga cgcaccaatc ggcggcacgt
      541 ctcttggggt tctcatattc caggatatcg cggtggtgcc gatgatgatc gtgacaccga
      601 ttctcgcagg gtccgagagc ctcacctttc agtctctcag cgttctcgtg ctgaagatcc
      661 tcgcagtaat cattatcgtg atcgtcgcgg ctcgttggct cgtgccacac gggctctaca
      721 gaatcgctcg aacgcgcgac aacgatctat tccttctcgc ggtggtcttt cttgccttcg
      781 cgacggcgtg gctcacggcg caagtcggtc tctcacttgc gctcggagcg ttccttgccg
      841 ggctcattat cagcgagtcg gaatatagcg aacgcgcgct cggtagtgtt ctcagtttcc
      901 gcgacgtctt cacgagtttc ttcttcatat caatcgggtt gctgatggat gtcagaatcc
      961 tttggcaatc accggtgctc gtttttggtt tcactgtcgg cgtgatcctc ttaaaaatcg
     1021 ttacaacggt gtctgccgtt gccatacttg ggttaccgtt tcggacggcg gcgctcaccg
     1081 gcgtggcgct gagtcaaatg ggcgagtttt cgttcatcct ttcgaagccc ggaatcgaaa
     1141 caggtctgct cactcccgag cactatcaaa tatttctcgc ggtatccatt ctcacgatga
     1201 tcgcggcacc gttcatgatc atgcacgcac cggcaatcgc cggtttgatt gagcgattac
     1261 cgattcccga tcgtgtccgc cgaggtatca cgaaacagcc acccgaagca ccggaaagcg
     1321 atctttctga acacctgatc gtcgtcgggt acggactcac cggtcggaac gtcgtccgcg
     1381 ccgcaatcga agcgctcatt ccgtacgaaa tcatcgaact gaaccccgac accgtacacg
     1441 ccgaacggca gaacggcgaa gtgattcact acggcgacgc gtctcagcca aacgtgttgt
     1501 tgtatgccgg tatcaagcgg gcgaaggtat tcgtaatcac gattccggat gcggtgtcaa
     1561 cgcggcggat cgtcgaagtg gcgcgacgga tgaatccgga cctgcacatt atcgcgcgca
     1621 cgcggtacgt tcgcgagatg gaaacgttat acgcgctcgg tgcaaacgaa gtgattcccg
     1681 aagaatatga gacgtcggtg gagttattct cgcgggtcat gcactcgtat ctcgtaccgt
     1741 acgacgtgat cgaccgattc atcgccgagg tgcgcgccga tagttacgaa atgctgcgcg
     1801 acgcaccggc gaccgcacca atacacattg ttcgacatct ccaagggctc gatatcacca
     1861 cgttccaggt cgcgaatggc gcatcggtcg tcggtcagac cctcgcggag atcggtttgc
     1921 ggaaaaacca caaagtgacc gcgcttgcca ttcatcgggc cggtgaaaag atcaacgttc
     1981 ctgacggcac cgccgagttt cacgcgggcg atctcgtcgt tgtcgccgga tgcccggacc
     2041 gtctcgccga agttgctccg ttgttccgcg caccggcgga tgcgtcgtcg gagactgagt
     2101 aaccgccgtt cactgaggtc accatgacag cgtcctacag cctcgacggg gagtggaccc
     2161 tccgcggatt ccctgagcgc tcccacaccg tcgattctcc cgacatgctc gacgatctcc
     2221 ccgtcgaacc gatttccgcg caggtccccg gcaacgtcga gctcgacctc gtgcgagccg
     2281 gcgtcatcga ttacgacccg tatgtcggcg acaacatcct gcggcttaaa gagtacgaag
     2341 ggtgggagtg gtggtatcgc cgttcgttcg acgggcctac ggaaaccggc aaacgactga
     2401 cgctacgctt cgacggcctc gactgtatcg ccgatatatg gttgaacgcg atgcatctgg
     2461 gacgacgaga gaacatgctc attccgcagg agttcgacgt cacggatatc atctcgcgcg
     2521 gcg
//
LOCUS       DSCC01000097            1619 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1051368, whole genome shotgun sequence.
ACCESSION   DSCC01000097 DSCC01000000
VERSION     DSCC01000097.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1051368"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..516)
                     /locus_tag="ENN56_01135"
     CDS             complement(<1..516)
                     /locus_tag="ENN56_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74120.1"
                     /translation="MKRKVFQIERCATFVSVFLCAIMGFTDLAHAEGDSMPVDSVSAV
                     GQFDVASSTSATRSHTCSPRTAFALGVLVPGGGQFYLRDWSGGIQAVVTDAILIEAMR
                     RSEATIGSMAFFIGAVHVVEGLFAAYECRTRTSVTAARNNGTLETHALSIVPGFLESR
                     VDRHEQTTGHRK"
     gene            715..>1619
                     /locus_tag="ENN56_01140"
     CDS             715..>1619
                     /locus_tag="ENN56_01140"
                     /inference="COORDINATES: protein motif:HMM:PF05378.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDS74121.1"
                     /translation="MAIALGLDAGGTYTDAVLYDLDTNTLLASAKAPTTPPDYVRGVA
                     EAVDALPLEIRATAEFVALSTTLATNAIVEHRHAPAGLVLIGYDEYESRSVNWNLKRR
                     VSGRHSIRGEETEPLDEAGLRAAVVDLLNEGVAGIAISGVMSTRNPDHELRALDVTAS
                     MCDLPIVAGHRVAGVLNAIVRAETAALNAGLIPIIHRFIDAVESVLASHGVANAPVFM
                     VTGDGSLMSSTAARVRPVETILSGPACSALGAARLSGVGDAVVADIGGTTTDIAVLEG
                     GTPRMSAGGVSVGSWRAGVRSAQVLT"
BASE COUNT          337 a          468 c          464 g          350 t
ORIGIN      
        1 cttcctgtgt cctgtcgttt gttcgtggcg atcaacgcga gattcgagaa accctgggac
       61 gatactgaga gcgtgtgttt cgagcgtgcc gttgttacgt gccgcagtca ccgatgtccg
      121 cgtgcgacac tcatatgcgg caaaaagccc ttcaacgaca tgaacggctc caatgaagaa
      181 tgccatactg ccaatagtcg cttcgctcct acgcatcgct tcaatcagga ttgcgtcggt
      241 aaccaccgct tggattccgc cgctccagtc gcgcaggtag aactgtccgc caccgggaac
      301 aagtacacca agtgcaaagg ccgtccgtgg tgagcaagtg tggcttcgcg tggcggaagt
      361 gcttgatgca acgtcgaatt gacccacggc gctgaccgaa tcgaccggca tcgaatcgcc
      421 ttccgcgtga gcgaggtcgg tgaatcccat gatcgcgcaa aggaaaaccg acacgaaggt
      481 cgcgcaccgc tcgatttgga atacttttcg tttcataccg attgagaata ccgcatcacg
      541 catgtcggtc aaaatgccca ctacgagaat cgataccgta gaccgtcgct ttccgactac
      601 ttcacgccga gaacgatgtc gttggtgcca aacacgatgc gcgtgtatct ttattctctt
      661 catgttcgat gacacacgta acactgagtg atccctcttt acgcgagatt ctccatggca
      721 atcgcacttg gtctcgacgc cggtggaacg tataccgacg ccgttcttta cgacttggat
      781 acgaatacac tacttgcatc agccaaagct cctacaacgc cgcccgatta cgtgcgcgga
      841 gttgccgagg ccgtcgacgc gcttcctttg gagatccgag cgacagccga attcgttgcg
      901 ctttcgacga cactcgcgac aaacgcgatt gttgaacatc gccatgctcc tgcgggtctc
      961 gtgctcattg gatacgacga atacgaatca cggagcgtca actggaacct gaagcgtcgt
     1021 gtttccggac gccactcgat tcgaggtgag gaaaccgagc cgctcgatga agccggactt
     1081 cgcgccgcag tagttgattt actcaacgaa ggagtagccg gaatcgcgat ctccggcgtg
     1141 atgagcacgc ggaatcccga tcatgagcta cgcgcgctcg atgtcaccgc gagtatgtgc
     1201 gacttgccga ttgtggccgg gcaccgcgtg gccggagtgt tgaacgccat cgttcgcgct
     1261 gaaaccgccg cgctcaacgc cggattgatt ccgatcattc atcgattcat cgatgccgtc
     1321 gaatcggttc ttgcgagcca tggtgtcgca aacgcgccag tgttcatggt gacgggcgac
     1381 ggtagtctga tgtcgagtac ggcggcacgg gtacgtccag tcgaaacgat actcagcggt
     1441 ccggcgtgca gtgccctcgg cgctgctcgt ttgagtggcg tcggcgatgc cgtcgtagca
     1501 gatattggcg gcacgacgac tgatatcgcc gtgctcgaag gcggcacacc ccggatgtcg
     1561 gcaggcggcg tatccgtcgg gtcgtggcgt gcaggagttc gctctgcgca ggttctcac
//
LOCUS       DSCC01000098            1725 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047087, whole genome shotgun sequence.
ACCESSION   DSCC01000098 DSCC01000000
VERSION     DSCC01000098.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047087"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..539)
                     /gene="murI"
                     /locus_tag="ENN56_01145"
     CDS             complement(<1..539)
                     /gene="murI"
                     /locus_tag="ENN56_01145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDS74122.1"
                     /translation="MDRRPIGVFDSGVGGLTVVAELQRQLPNERVVYFGDTARFPYGS
                     KSIATVTEFARQDSRLLLSHDVKLIVVACNTATAYSLDVLQQELDVPVIGVIDPGARA
                     AANASRRRLVGVIGTEGTIASRAYEQAIARIDPSISVFAQACPLFVSLAEEGWTDTAA
                     TLLIAQEYLEPLRESLIDVL"
     gene            complement(649..1692)
                     /locus_tag="ENN56_01150"
     CDS             complement(649..1692)
                     /locus_tag="ENN56_01150"
                     /inference="COORDINATES: protein motif:HMM:PF01520.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDS74123.1"
                     /translation="MATIRVPGDLIIEEDTRLPHWLDLTIIGGRIDAQAFQHVLLDGA
                     VQQIVAYQYQNSAVLSFRIEPGYRYDTMRSTSSGWLMVLFRREMPLPSPLAQVGELVE
                     IDREQWSINTIVIDPGHGGRDPGAVCDDNVYEKDIVLNIANRLARMLESELDVNVVQT
                     RSTDQFVSLRERGRIATQNGGKLFISIHANTNPDSRISGVETYFLSEAQTDEARQVAR
                     LENAAMRYEHTDEPVDGAPYSDWYAWQDVDEILAGMTSTRFLTESQELAKLVQDELAR
                     TLGARDLGVRQAEFYVMKGTLASMPSILVEVGFLSNPAEARHLRQAAYQRRAADAIFR
                     AVREFKQRYERNL"
BASE COUNT          364 a          507 c          486 g          368 t
ORIGIN      
        1 agtacatcga tgagcgactc ccgaagcggt tcgaggtact cctgagcgat aagcaacgtc
       61 gccgcagtat cggtccagcc ttcttcagcg agtgaaacga aaagtggaca tgcttgtgcg
      121 aacacgctga tcgaagggtc gatacgtgca atcgcttgct cgtacgcgcg agacgcgatg
      181 gttccttcgg taccgatcac gccgacgagt ctccggcgac tggcattcgc ggccgcacgc
      241 gcgccggggt cgattacacc gatcaccggt acgtccaatt cctgttgaag cacgtcgagt
      301 gaatatgccg ttgccgtgtt acatgcgacc acgatgagct ttacgtcgtg cgacagaagg
      361 agacgcgaat cctgccgcgc gaattcggtc accgtcgcaa tggatttgga gccgtacggg
      421 aagcgcgccg tgtcaccgaa gtatacgacg cgttcgttcg gtagctgtcg ttgcagttcg
      481 gcgacgacgg tgagcccgcc gacaccggaa tcgaatactc caatcggtcg ccggtccaga
      541 gcaaaatcct ccacgataac gtaatcgaag cgtaaaatga taacacggta cggtataatg
      601 tgcggaacag agagggtgtc ggccaaccgg gaagcgaagg cgtgatcgtt ataaattgcg
      661 ctcgtaacgt tgtttgaact cgcggacggc ccgaaagatt gcgtcggcgg ctcggcgttg
      721 atacgcagcc tgacgaaggt ggcgcgcttc ggccggattc gagaggaatc cgacttcgac
      781 gaggatactc ggcatcgacg cgagcgtccc cttcatcacg tagaactcag cttgccgcac
      841 gccgaggtcg cgcgcgccga gtgttcgcgc gagctcatcc tgcacaagct tcgcaagttc
      901 ctgactctcg gtgaggaacc gggtcgaagt catccccgca agaatctcgt cgacatcctg
      961 ccatgcatac cagtcggaat acggcgctcc gtctacgggt tcgtcggtat gttcataccg
     1021 catcgccgca ttttccaacc gcgcaacttg gcgcgcttcg tcagtctgcg cttccgaaag
     1081 aaaatacgtt tcgactcccg atattcgcga atcaggattc gtgttcgcgt gaatactgat
     1141 gaacagtttt ccaccgttct gcgtggcgat tctaccacgt tctcgcagac tcacaaactg
     1201 gtcggtcgag cgtgtctgca cgacgtttac atcgagttcc gattcgagca ttcgcgccag
     1261 acgattcgcg atgttcaata caatgtcttt ctcgtaaacg ttatcgtcgc atacggcccc
     1321 gggatcacgc cccccgtgcc ccggatcgat gacgatcgtg ttgatcgacc attgctcgcg
     1381 gtcgatctcg accaattcgc caacctgcgc gagcggcgat ggtagcggca tctcgcggcg
     1441 gaagagcacc atgagccaac cgctcgatgt gctccgcatc gtatcgtatc gatatcccgg
     1501 ttcgattcgg aagctcagca cggccgaatt ctgatattga tacgcgacga tctgctgcac
     1561 cgcgccatcg agtaatacat gctgaaacgc ctgtgcgtcg atacgcccgc cgatgatcgt
     1621 cagatcgagc caatgcggaa gccgtgtatc ctcttcgatg atgaggtcgc ccggcacgcg
     1681 aatcgttgcc aatgtaccgt tctctcgctc ctcaatcgtt ccggg
//
LOCUS       DSCC01000099            2337 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031127, whole genome shotgun sequence.
ACCESSION   DSCC01000099 DSCC01000000
VERSION     DSCC01000099.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031127"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="ENN56_01155"
     CDS             complement(<1..1577)
                     /locus_tag="ENN56_01155"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:PRK13981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HDS74124.1"
                     /translation="MQNLRLALAQINTTVGDIRGNTHLMLDWIDRAREAHADVVAFPE
                     LATIGYPPEDLLFRPDLLRENLKARDEVVAASNGMVVVFGFVDAPDDIYNAAAIACDG
                     SLAGVYHKEFLPNYGVFDENRYFQAGIGRRVYEIDGVILGVNICEDIWYPGGPTDDQA
                     LIGGAHVIVNLSASPYHRGKIGTRDRIIATRASDNDAVVALVNLVGGQDELVFDGGSL
                     ICDETGAILARGKQFEEDLIVADVSLDAIFRYRLRDSRRRKEKYSAMLRGREIERISL
                     DIVPSPSRKAVEPHLTPRCDLLTETYRALTLGLRDYVEKNRFKSVVVGLSGGIDSALT
                     VAIAVDALGADRVVGVTMPSRYSSEETRDDARIIAENLGIKFLTIPIEDAYTTYIRTL
                     EDVFTGYPPDVTEENIQARIRGNLIMALSNKFGWLVLTTGNKSEMSVGYATLYGDMAG
                     GFAVLKDVYKTTVFELSLWRNAQGDGEVIPNTTIERPPTAELRPNQKDEDSLPPYAVL
                     DPILEAFVERDETVDEIV"
     gene            complement(1619..2260)
                     /locus_tag="ENN56_01160"
     CDS             complement(1619..2260)
                     /locus_tag="ENN56_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74125.1"
                     /translation="MFRHTTVTLITCGMLFSVVCVPTATGAVDMLWTVAGGIGYLPDA
                     SSDVAASPSVHGTACIRLTERTWLGVHGDYTSYRPVKADEAGSQWRVLALVQWEWRHP
                     WMPSQMTSFAQFGIGFGRMAYTDALPPTESLRLWYPEQVTYGPAARLAGGFWWTVHPR
                     IRANATVSFERLDTAINAISRKYPTMIGLTIGVSIQLPRGSSLTGFDEAITRE"
BASE COUNT          518 a          703 c          610 g          506 t
ORIGIN      
        1 acgatctcgt caaccgtctc atcgcgttcg acgaacgcct cgagaatcgg atcgagtact
       61 gcatatggtg gcaggctgtc ctcgtccttc tggttcggtc gtagctccgc cgtaggtggt
      121 cgctcaatgg tcgtgttcgg aatcacttca ccgtcaccct gtgcgttgcg ccaaagggaa
      181 agctcgaaca cggtcgtctt gtaaacgtcc ttcaacacgg cgaatccacc cgccatatca
      241 ccgtagagtg tggcataccc aacggacatc tcagatttgt ttccggttgt gagaaccagc
      301 catccgaact tattcgacag cgccatgatg agatttccgc gaatccgcgc ttgtatgttc
      361 tcctcggtga catccggcgg gtacccggta aacacatctt cgagtgtcct gatgtacgtc
      421 gtatatgcgt cctcaatcgg aattgtgaga aacttgatgc cgaggttctc cgcgatgatc
      481 cgggcatcat cacgtgtttc ttccgagctg taccgcgacg gcatcgttac gccgacaacc
      541 cggtcagctc ccagcgcgtc cacggcaatc gcgacggtaa gcgccgagtc gataccaccc
      601 gaaagcccaa ccacaacgct tttgaaacga tttttctcta cgtagtcgcg aagacccagt
      661 gtgagcgctc gatacgtctc ggtgaggagg tcgcaacgcg gcgtcaaatg cggttcgacg
      721 gccttgcgac tcggcgacgg cacgatgtcg agtgagattc tttcgatttc ccgaccgcgc
      781 agcatcgctg aatacttttc cttccgacgc cgcgaatcac gcagccgata ccggaaaatc
      841 gcgtcaagtg acacatccgc gacgatgagg tcctcttcaa actgctttcc gcgagcgaga
      901 attgcgccgg tctcatcgca gatcagactc ccgccgtcga acacgagctc atcctgtcca
      961 ccgacgaggt tcaccagtgc aacgacggcg tcgttatctg atgcgcgagt cgcgataatc
     1021 cgatcacgcg taccgatttt tccacgatga tagggcgacg cgctcagatt gacgatcacg
     1081 tgggctccgc cgatcagcgc ctggtcatcg gttggtccac cgggatacca gatatcctcg
     1141 cagatattta ctccaagaat gacgccgtcg atttcgtaaa ctcgtcgccc aatacccgct
     1201 tggaaatatc ggttttcatc gaatacgccg taattcggga ggaactcctt gtgatacacc
     1261 cccgcaagcg atccgtcaca cgcgatcgcg gcggcgttgt agatatcgtc cggcgcatcg
     1321 acgaatccga acacgacgac catgccgttg ctcgccgcga caacctcatc acgcgctttg
     1381 agattctcgc gcagaaggtc cggacgaaac agaaggtctt cggggggata gccaatcgtt
     1441 gcgagttctg gaaatgcgac gacatccgca tgggcctccc gcgcacggtc gatccaatcg
     1501 agcatcagat gtgtgttacc gcggatatct ccaacggttg tattgatctg agctaacgcc
     1561 aatcgaaggt tttgcatttt ccgcctgcaa tgatgtattc agtgactccg ataccgttct
     1621 actcgcgagt gatcgcttcg tcaaatcccg tcagggaact accgcgcggc aactggatcg
     1681 acacgccgat agtgagacct atcattgtcg gatacttgcg cgagatcgcg ttgattgctg
     1741 tatccagccg ctcgaagctt accgttgcgt ttgcgcggat acgtggatga accgtccacc
     1801 agaatccacc agccagtcga gccgccggac cgtacgttac ctgctcggga taccataaac
     1861 gtaacgattc ggtaggcggt aaggcatcgg tatacgccat cctaccaaag ccgatcccga
     1921 actgcgcaaa cgatgtcatc tgcgatggca tccacgggtg gcgccattcc cattgtacaa
     1981 gcgccagaac acgccactga gacccggctt cgtctgcttt cacgggtcgg tacgacgtgt
     2041 aatcaccgtg aacgcccaac cacgttcgtt cggtcagccg gatacatgcc gttccgtgta
     2101 ccgatggcga cgcggcgaca tcggaggaag cgtcgggaag atatccgatt ccacccgcaa
     2161 ccgtccacag catatcgacg gcgcccgttg cggtcgggac acagacgacc gaaaacagca
     2221 taccgcaagt aatcagcgtc accgtcgtat ggcgaaacat attccgcacg aatcctcgat
     2281 ccacaagtcg ctttatatct gtacgtaagc tacggatgca tggggtcacg tcaatat
//
LOCUS       DSCC01000100            2058 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037035, whole genome shotgun sequence.
ACCESSION   DSCC01000100 DSCC01000000
VERSION     DSCC01000100.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037035"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..224)
                     /locus_tag="ENN56_01165"
     CDS             complement(<1..224)
                     /locus_tag="ENN56_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_636523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74126.1"
                     /translation="MAQDFISVRGARVHNLRNVDLSLPKNRLIVFTGVSGSGKSSMAF
                     DTIYAEGQRRYIESLSAYARQFMDQMEKPDV"
     gene            514..1617
                     /locus_tag="ENN56_01170"
     CDS             514..1617
                     /locus_tag="ENN56_01170"
                     /inference="COORDINATES: protein motif:HMM:PF08240.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74127.1"
                     /translation="MRREVGMKGTMRAAVLKTPGNLAVEEIPIPEIESDELLIEVAGC
                     GICGSDLRYFAGENPWAQHTLGKAEPNPPNMVLGHEFGGTVVEAGSTRLESWVGRRVV
                     VAPFDVSGFDRASRLGKPHLSTDTVHIGHGAGWGEMDYYPGAMAQYCRAWARRCYELP
                     DALSFDVAAVLDIAGVAYHALEVASFASGKSVAVIGMGPLGVSAVRLARLSGASHAVA
                     IDIFDAPLEFALTSGAEVFNTRRQSDWKRKILDFTAGWGVDAVLDTVGTADTLRDGSD
                     LLAPDGTLVLLAVHAGTISLPITALAGERKITTSANYRYEDFQRTIDLTASGRLPLDD
                     LVTHHVPLSRVHEGFDILRNKETTGAMKVIVTP"
     gene            1813..>2058
                     /locus_tag="ENN56_01175"
     CDS             1813..>2058
                     /locus_tag="ENN56_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS74128.1"
                     /translation="MSGHSKWANIKHKKGRADAERGKIFTRITKEIVVAARVGGGDPT
                     MNPRLRAAMDKARQANMPNDNIERGVKRGTGELEGVTY"
BASE COUNT          449 a          564 c          620 g          425 t
ORIGIN      
        1 acatcgggct tttccatctg atccatgaac tggcgggcgt acgcggagag cgattcgata
       61 taccggcgtt ggccttcggc gtagatcgtg tcgaacgcca tactgctctt gcccgacccg
      121 gaaacgccgg taaagacaat gagccggttt ttcggaagcg agaggtctac gttgcgaaga
      181 ttgtgaacgc gcgcgccgcg aacactgata aaatcctggg ccattgaaac gccgtgagtc
      241 tttcgcgaaa ttctcgtggt atgccttttc ggtatacatt ccgagtgtgt acagaatacg
      301 ggtagttacc cacaatcagc aaggtgcgct gtactatcga tacagtggcg catatcttga
      361 tgttccatgc ctgcgataca gcggcgatat tacgggtata tttccacggt tcggctcgcc
      421 atgcgacttg ccgccgacga tatcgctgcg tagtctccga atgtagcaaa tccgaagcat
      481 gatggcgtat tccagtcacg ttgcgaaccc aggatgcgcc gggaggttgg tatgaaaggc
      541 acgatgcggg ccgcggtgtt gaaaacgccc ggaaatctcg ccgtcgaaga aatcccgata
      601 cccgaaatcg aatccgatga gcttctgatt gaagttgcgg gttgcggtat ttgcggatcg
      661 gatctcaggt atttcgcagg cgaaaacccg tgggcacagc acactcttgg gaaagccgag
      721 ccgaatcctc cgaacatggt gctcggacac gaattcggcg gcaccgtcgt cgaagcggga
      781 tcaacacggc tcgaatcatg ggtggggagg cgggtagtcg tcgcgccgtt tgacgtatcg
      841 gggttcgatc gggcctcacg tctcggtaag ccgcatcttt ccaccgatac ggttcacatc
      901 ggccacggcg ccggatgggg cgaaatggac tattatcccg gcgcgatggc gcagtattgc
      961 cgtgcctggg cccgtcggtg ttacgagttg ccggatgccc tatcgttcga cgtggccgcg
     1021 gtactggata tcgccggcgt tgcctaccat gcgctcgagg tcgcctcgtt cgcgtcgggc
     1081 aagtcggtcg ccgtcatcgg aatggggccg ctcggagttt ccgccgtgcg actcgcgcgc
     1141 ctgagtggcg cttcgcacgc ggtggccatc gatatcttcg acgcaccact tgaattcgcg
     1201 ctaacttccg gcgcggaggt attcaacacg agacggcaat ccgactggaa acggaagatc
     1261 ctcgatttca ccgccggatg gggcgtcgat gcggtgctcg acacagtcgg aaccgccgat
     1321 acgctccgcg acgggagtga tttactcgcg cccgatggta cattagtact cttggcggtg
     1381 catgccggaa cgatctcgct accgataacc gcgcttgcgg gagagcggaa gatcaccacg
     1441 tcggcaaact atcgatacga ggatttccag cgcaccatcg atctcaccgc ttcgggaaga
     1501 cttccgctcg atgacctcgt gacgcaccat gtgccgcttt cacgggtgca cgagggattc
     1561 gatattttac ggaataagga gacgaccggc gcaatgaagg tcatcgttac gccgtgaaga
     1621 cgattccgca attctgcgtc agcgtatgga gaggcgactg aatggttcga tcgagttcac
     1681 catttcgaag actccgactg attctcatca gagtcagcca caaacgtcct ccagggcatt
     1741 tccccgcgta ctacgtatct gcgtgtggtg aatagtactt gaaatcagac gaccactcag
     1801 gaggacacga tcatgtcggg ccattcaaaa tgggcgaaca tcaagcacaa gaagggaaga
     1861 gccgacgctg agcgcggcaa gatatttacg cggatcacga aggaaatcgt cgtcgcggca
     1921 cgagtcggcg gcggcgatcc gacgatgaat ccacgccttc gcgctgcgat ggacaaagcg
     1981 cgtcaggcga acatgccgaa cgataacatt gaacgcggtg tcaagcgtgg aaccggcgag
     2041 ctcgaaggcg ttacgtac
//
LOCUS       DSCC01000101            1644 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1050273, whole genome shotgun sequence.
ACCESSION   DSCC01000101 DSCC01000000
VERSION     DSCC01000101.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1050273"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..414)
                     /locus_tag="ENN56_01180"
     CDS             complement(<1..414)
                     /locus_tag="ENN56_01180"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS74129.1"
                     /translation="MGERRGATPRRVRETPVLNGLSVVICSVVIPTYNRPDQIAAALD
                     ALAAQYESDAPFAVIVVDDGSEPALSISNRDRPFPVNLVRLTENRGRAAARNAGISAV
                     DTPLTVFLDDDIRVEPGYMRAVQNRIDPEGSEIGIG"
     gene            complement(342..1496)
                     /locus_tag="ENN56_01185"
     CDS             complement(342..1496)
                     /locus_tag="ENN56_01185"
                     /inference="COORDINATES: protein motif:HMM:PF00534.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS74130.1"
                     /translation="MTEQRRRRRVAMVVYNDLVHDARVYKTASTVRDAGYDVHVIGMV
                     APESGPLGAWDGIRVTRLDVGVRRSLRVRYAQFWRAAYRVLRADTPDVIHANDLDVLV
                     PCWLAARKLRIPIVHDAHELWVELPSLVGRPHVRAIWSAVAHALISRCDAVITVSDGV
                     AGEIRRRYGVEPTIVRNLPIRGGPVEPAPLRDRLGIPDDAPILIYQGGLLPGLGIERA
                     VDAMDFLPQAHLVIIGGGPLRELIARHVEQSPSRDRINLLPPVPFAELKPITTACDIG
                     LYLGSSAGLNLQYALPNKLFEYIAAHVPSVITDWPDQAAIVRHYGVGTVVPPNASPEH
                     VAGAVRDTLANRDSYVANCRVAANELVWENDAARLLDVYAKLPYSTDSLS"
BASE COUNT          345 a          506 c          484 g          309 t
ORIGIN      
        1 cccgatgccg atctcgctgc cctctggatc tatccggttt tgcactgcgc gcatgtatcc
       61 cggttcgacg cgtatatcgt catcgagaaa gacggtgagc ggcgtatcca cggcggagat
      121 gcccgcgttt cgcgccgccg ctcgaccgcg gttttctgtc aatcggacga ggttcacggg
      181 aaacggtcgg tcgcggttcg aaatcgatag cgccggctcg gaaccatcat ccaccacaat
      241 gacggcgaat ggagcgtccg actcatactg cgccgcgagc gcgtcaagcg ctgcggcaat
      301 ctgatcggga cggttatacg tcggaatgac gacgctgcat atcacgacag agagtccgtt
      361 gagtacggga gtttcgcgta cacgtcgagg agtcgcgccg cgtcgttctc ccacacgagt
      421 tcatttgctg cgactcggca attcgcgaca tagctgtctc ggttcgcgag agtgtcacgc
      481 actgcaccgg caacgtgctc cggtgatgca ttcggtggca cgacggttcc aacgccgtag
      541 tgacgaacga tcgcggcttg atccggccaa tccgtgatca ccgatggcac gtgggccgcg
      601 atatattcga acagtttgtt cggtaacgcg tactgcagat tcaatcccgc tgacgaacca
      661 agatacaatc cgatatcaca cgctgtcgta atcggcttca gctccgcaaa cggcaccggc
      721 ggaagcagat tgatccggtc acgcgagggc gactgttcaa cgtgtcgcgc gatcagctcg
      781 cggagtggtc ctccgccgat gatgaccaag tgcgcttggg gaagaaagtc catcgcgtcg
      841 acggctcgtt cgatgccgag ccccggaagc aaaccacctt ggtagatcag gatcggagca
      901 tcgtccggta tgccgagacg gtcacgaagc ggcgcaggtt cgaccggacc gccgcgtatc
      961 ggtagattac gaacgatggt cggctcgaca ccataccgtc gacgaatctc tcccgcgact
     1021 ccgtccgaga cagtaatcac ggcatcgcaa cgtgagatga gcgcgtgtgc aaccgctgac
     1081 cagatcgcac gcacatgcgg gcgtccgaca agcgacggta gttcgaccca taactcatgt
     1141 gcatcgtgga cgatcggtat gcgaagcttt cgtgccgcga gccaacatgg cacaagcaca
     1201 tcgaggtcgt tggcgtggat cacgtccggc gtatcggcgc gaagcacacg atacgctgcg
     1261 cgccagaatt gcgcgtaccg aacgcggagt gaccgccgca caccaacatc caaccgggtg
     1321 acacggatgc catcccacgc tccaagcggc cccgattcgg gcgctaccat gccgatgaca
     1381 tgaacatcgt atccggcatc tcgcaccgtc gacgccgttt tatacacgcg ggcgtcatgg
     1441 acaaggtcgt tgtagacaac catcgcgacc cgacggcgtc gtcgttgctc ggtcatcgaa
     1501 accaccttgc gacacggttt ttcagtcgcg gagcgcccgt agcgaggaag cgatggtagc
     1561 gcgatcgcga ccacgacacc gcatcgtacc gaacgagttc accgccgaat ccgcgtttga
     1621 actttgcgat gctctccata ttcg
//
LOCUS       DSCC01000102            2203 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033753, whole genome shotgun sequence.
ACCESSION   DSCC01000102 DSCC01000000
VERSION     DSCC01000102.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033753"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            184..1194
                     /locus_tag="ENN56_01190"
     CDS             184..1194
                     /locus_tag="ENN56_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDS74131.1"
                     /translation="MGSEEKLARHRLSVLELAVQLGNVSEACRRRGMHKSQFYEWKRR
                     FQTHGLEGLKDLPPIHKSHPMTTPAGVVKRVVALSLEHPAWGCTRLSDYLKLDGVSVS
                     SPTVQKILIKHGMASRYDRWLKLEEQRGGGAIELTGEQVAFIEKQNPAFRERRVESSR
                     PGELLCQDTFFVGHLKGVGKVWLHAVVDTYGSYAFGFLHTTKTPEAAVAPLHNDVLPF
                     YRNHELPVGAILTDNGREYCGTDAHSFELYLALNDIEHRTTKVKTPRTNGFVERFNCT
                     VLDEFFRQAFRTTFYNTVEALQIDLDAWLVHYNTERPHQGYRNMGRRPIETVQRYIKK
                     SK"
     gene            complement(1291..1755)
                     /locus_tag="ENN56_01195"
     CDS             complement(1291..1755)
                     /locus_tag="ENN56_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015892227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS74132.1"
                     /translation="MREHQTDILIVGGGVGGCAAAVAGTSLGYRVILTEETDWLGGQI
                     TSQAVPPDENRWIEMFGCTRRYRAFRNGVRQYYREHYPLTPDARANARLNPGGGRVSA
                     LCHEPRVAVAVIEAMMAYPRTAGLLNVLSTERCASTRCARRRSAVGEFPRAV"
BASE COUNT          471 a          631 c          638 g          463 t
ORIGIN      
        1 ggcttaaaaa tcagataggt agtctaatcg ttcccagatt catagtattt atgtctggaa
       61 catcgcaata tatggtgcga atggtgcagt cagattgagg gattaaatct tactcagctg
      121 tcctgctaag cttctctttg gtttgtagac gggagtatct ttggttgaga aggaggagct
      181 gagatgggat cggaggagaa gcttgcgcgt caccggttga gcgtgttgga gctcgccgtg
      241 caactgggga acgtgtcgga ggcgtgtcga cgtcggggga tgcacaagag ccagttttac
      301 gagtggaagc ggcggttcca aacgcacggg ttggaggggt tgaaggatct tccgccgatc
      361 cacaagagcc acccgatgac cacccccgcg ggggtggtca agcgggtcgt ggcgttgagt
      421 cttgagcacc cggcatgggg ctgcacgagg ttgagcgatt atctgaagct cgacggggtg
      481 tcggtgagtt cgccgacggt acagaagatc ctgatcaagc acggcatggc gagccggtat
      541 gatcggtggc tcaagctcga agaacaacgt ggtggcggag cgatcgagtt gaccggcgag
      601 caggttgcgt tcatcgagaa gcagaacccg gcgtttcggg agcgtcgggt ggagagtagt
      661 cgtccgggtg agctgctgtg ccaggacaca ttcttcgtgg ggcacctcaa gggtgtgggg
      721 aaggtgtggc ttcatgcggt tgtcgatacc tacgggagct acgcgttcgg cttcctgcac
      781 accacgaaga cgcccgaagc cgccgttgcg ccgctgcaca acgacgtcct gccgttctac
      841 cggaaccacg aactccccgt cggggcgatc cttaccgaca acggcaggga atactgcggc
      901 accgatgccc attccttcga gttgtatctg gcgctcaacg acatcgaaca tcgcacgaca
      961 aaggtcaaaa caccgcgtac caacggcttc gtcgagcgat tcaattgtac cgtcctcgac
     1021 gagttcttcc gccaagcgtt ccgaactaca ttctacaata ccgtcgaagc cctccagatc
     1081 gacctcgacg cctggctcgt tcactacaat accgaacgtc cacaccaggg ataccgaaac
     1141 atgggaagac gaccaatcga aactgttcaa cgatatatca aaaaaagtaa atgaagacgc
     1201 ttagcagaac acatcacgga atcaatactt tgcccgagtg gtcgctacac cgcatacgtc
     1261 ttcggccatt cgcgttcaat gccctgatca tcacacagcc cttggaaatt cgccaaccgc
     1321 gctgcgtctt cgcgcacacc gcgtggatgc acatcgttcc gtagacaaca cgttaagcag
     1381 ccccgccgta cgcggatacg ccatcatcgc ttcgatgacg gctacggcaa ctcgtggctc
     1441 gtggcagagc gcggagactc ggccaccgcc gggattcaat cgggcatttg cgcgagcgtc
     1501 aggtgtgagc gggtaatgtt cgcggtagta ctgccgaacg ccgttacgga atgcgcggta
     1561 acgccgcgta caaccgaaca tttcgatcca acggttttca tccggcggca ccgcctgcga
     1621 ggtaatctgt ccgccaagcc aatcggtttc ctcggtgaga atcactcggt agccgagtga
     1681 ggttcccgca actgctgcgg cacatccccc gacgccgcca ccgacgatga gaatgtcggt
     1741 ttggtgctca cgcatcgtct gctcccgttt ttggttcatt caccacgatt cgatttccgc
     1801 gatcgcgagt ccggattcac cggcgaccga gtacacgaga taccaacgtc cgtcttcttc
     1861 gaacaacgcc gggtcgcgca actcgttcac cggtcctttc gcagaccctt gtctggacgg
     1921 cacaagcggt tgatttgcgc cttcccacgg ttcttccggg ctgaggacaa cctccgcgct
     1981 cgacggtttc cattcgcgcc agtctggttt cagatcgatc gtgcaatact tgatcgattc
     2041 gggcatgtcg ccgatatcgc tccatagcaa gtgaagcgtg tcgcctgcaa ctcggacagc
     2101 cgcgtggcga aagcgctcgt cgaatacctg cggaccttct tcgaatccgc tcagtccgtc
     2161 ccgcgaacga tgcgtgattc cgtcccgcgc gatggcgtaa tac
//
LOCUS       DSCC01000103            3067 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021680, whole genome shotgun sequence.
ACCESSION   DSCC01000103 DSCC01000000
VERSION     DSCC01000103.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021680"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..561
                     /locus_tag="ENN56_01200"
     CDS             <1..561
                     /locus_tag="ENN56_01200"
                     /inference="COORDINATES: protein motif:HMM:PF04452.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDS74133.1"
                     /translation="EKMFESGEPTVKVTLAAAIIKADRFESLVEKATELGVDAVWPME
                     SENSVVRAPSENKQRRWRAIAESAAGQCLRSRVPQIADPLMLDDVIARRGEFDAAFVA
                     WERQRNGSLESATLDSRKRILLIVGPEGGFTPVEIARLESANVTPVTLGARRLRTVTA
                     AITLLTLTMQWAGEFSRTAYGAIDSD"
     gene            601..2193
                     /locus_tag="ENN56_01205"
     CDS             601..2193
                     /locus_tag="ENN56_01205"
                     /inference="COORDINATES: protein motif:HMM:PF01401.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3A and M3B thimet/oligopeptidase F"
                     /protein_id="HDS74134.1"
                     /translation="MTHPAHQFIEQHIERVQALERDVALAYWEAALTGSEAAAKRRAE
                     LSARLTKIYANPEEFARLSEWREDGTLDELTARQVELLYRAYLGNQKDEATIERMSNL
                     EAEVEVAYSTFRGVLDGEKKTNNELRKVLTDDNDSEKRKRAWEASKDVGPVVRDRVLE
                     LVTLRNKVARDAGYPDYYHLSLFLQELDVTELFTLLDDLEQQTREPFRKAKNQLDAHL
                     CKRFDVRCDELRPWHYADFFFQDAPSNDEVDLYPFFRDKDLVALATQTYDEIGLDVRD
                     ILSRSDLYPKDGKDQHAFCINTNRADDVRVLCNLEPDESWMATLLHELGHGVYDENLD
                     ADLPWTLREPAHTLLTEAIAMIMGRLTKDQHWLRTTAGVASEDIDRIMPHLRQYSSLA
                     MLIFVRWCLVMTYFERDLYANPKQDLNTLWWDYVERFQMLRRPEGRDMPDWASKLHIA
                     LAPVYYHNYLLGEMVASQIHHYITKHVTPVGAQGSPKVGQYLRDRLFRLGARYPWNQT
                     IEMVTDEPLTSRHFSEQFVTQA"
     gene            2203..>3067
                     /locus_tag="ENN56_01210"
     CDS             2203..>3067
                     /locus_tag="ENN56_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDS74135.1"
                     /translation="MPTKRPNFIVFYLDDLGYGDLGCQGSDAIRTPHIDDLANRGVQF
                     RQWFSNSPVCSPSRAALYTGRHPMRAGVPHILGGKRGTPGLPPSERTIATHFRDNGYR
                     TGLVGKWHLGVTPETSPMAHGFEEFYGFKAGCIDYYSHIFYWAAYSGDDPVHDLWEND
                     KEVWENGRYFTDLIGDKAIDFIDRHADEEFFLSVPFNAPHYPMHAPREYLDRYPDLPP
                     DRRIMAAMVSAVDDNVGRIMASLQSHGLADDTVVFFSSDNGPSTETRNWLDGTPDKYY
                     GGSAGIFRGHKA"
BASE COUNT          690 a          911 c          890 g          576 t
ORIGIN      
        1 gaaaaaatgt tcgaatccgg cgaaccgaca gtgaaggtga ccctcgccgc ggccatcata
       61 aaggcggatc ggttcgagtc acttgtcgaa aaagcaaccg aactcggcgt tgacgcagtt
      121 tggccgatgg aatcggaaaa ctcggtggtg cgcgctccat cggaaaacaa gcagcgtcgc
      181 tggcgcgcca tcgcggaatc agcggcggga cagtgccttc gatcgcgcgt gccacagatt
      241 gccgacccgc tgatgctcga cgacgtgatc gcgaggcgtg gtgagttcga tgcggcgttc
      301 gtcgcgtggg agcgacagag gaacggctcg ctcgaatcgg cgactctcga ttcgaggaag
      361 cggatactgc tcatcgtcgg accggaaggt gggttcacgc cggtggagat cgcgcggctt
      421 gaatcggcga acgtcacacc ggtcactctg ggcgcgcgtc gcctgcggac ggttacggcc
      481 gccattactc ttctcactct caccatgcag tgggccggtg aattctcccg tacggcgtac
      541 ggggcgatcg attcggacta aaaacggttt cgtttcacat tacgaaaagg acgatacatc
      601 atgacacatc cggcgcatca gttcatcgag caacacatcg aacgcgtaca ggcacttgag
      661 cgcgacgtag cgctcgcgta ctgggaggcg gcattgacag ggagcgaagc tgcggcaaaa
      721 cgtcgcgccg agctcagtgc gcggttgacg aagatctacg ccaatcccga agaatttgca
      781 cggctttcgg aatggcgcga ggacggtacg ctcgatgaac taacggctcg acaggttgag
      841 cttctctatc gcgcgtatct cggaaaccag aaggacgaag cgaccatcga gcgcatgtcg
      901 aatctcgaag cggaagtgga agtggcgtac tcgacatttc gcggtgtgct cgacggcgag
      961 aagaagacaa ataacgagct acgcaaagtt cttaccgacg acaatgactc cgagaaacgt
     1021 aaacgcgcat gggaagcatc gaaggatgtc ggtcccgttg tgcgcgaccg cgtgctcgag
     1081 ctcgtaacgc tacggaacaa ggttgcgcgc gatgccggat acccagacta ctaccatctg
     1141 tcgctgtttc ttcaggaact ggacgttacg gaactgttta cgcttctcga cgacctcgaa
     1201 cagcagacac gtgagccctt ccggaaagcc aaaaaccagc tcgacgcgca cctgtgtaaa
     1261 cgattcgacg ttcggtgtga cgagctgcgt ccgtggcatt atgcggactt cttcttccag
     1321 gacgcgccct cgaacgatga agttgacttg tatccgtttt tccgcgacaa ggaccttgtg
     1381 gcgctcgcca cgcaaaccta cgacgagatc ggtctcgacg tgcgtgatat cctcagccgc
     1441 agcgacctct acccgaagga cggcaaggat caacacgcgt tctgcatcaa caccaatcgt
     1501 gcagatgatg tcagggtgtt atgcaacctt gagccggacg agagctggat ggctacattg
     1561 cttcacgaac tcggacacgg cgtatacgac gaaaacctcg acgcagacct accgtggacg
     1621 ctccgtgaac cggcccatac gctgctgacc gaggcgatag cgatgatcat gggacgcctc
     1681 acgaaggacc agcattggct gcggacgacg gccggtgtgg catcggagga catcgaccgc
     1741 atcatgccgc atctccgcca gtattcgagt ctcgccatgc tcatcttcgt ccgctggtgt
     1801 ctggtaatga cgtacttcga gcgcgatctc tatgcgaatc cgaagcagga cctgaacacg
     1861 ctgtggtggg actacgtgga gcgcttccag atgttgcgcc gtccggaggg ccgggatatg
     1921 ccggactggg catcaaaact gcacatcgca ctggcaccgg tatattatca caactacctg
     1981 ctcggcgaga tggtagcgag ccagattcac cactacatca caaagcacgt aacgccggtc
     2041 ggcgcgcaag gaagcccaaa ggtcggtcag tatttacgcg atcggctgtt tcgcctcggc
     2101 gcgcgatatc catggaatca gacgatcgaa atggtcaccg acgaaccgct cacgtcgcgg
     2161 cacttctcag agcagttcgt gacgcaagca taggagttat tcatgccgac gaaacgacca
     2221 aacttcatcg tattctacct cgacgacctc ggctacggcg acctcggctg ccaaggctcc
     2281 gatgccatcc gcacgccaca cattgatgat ctcgcgaatc gcggcgtcca attccgacag
     2341 tggttctcga actcgccggt ctgctctccc tcacgcgccg cgctctacac cggtcgacac
     2401 ccgatgcgcg cgggcgttcc gcatattctc ggcgggaagc gcggcacacc cggtctcccg
     2461 ccctcggaac gcacaatcgc gacacacttc cgagataacg gctaccgcac cggcctcgtc
     2521 ggaaagtggc atcttggggt gactccggag acctcgccaa tggcgcacgg ctttgaggag
     2581 ttctacggat tcaaggcggg ctgtatcgat tactactcgc acatcttcta ctgggccgcg
     2641 tactccggag atgaccccgt tcacgacctg tgggaaaacg acaaggaagt ctgggaaaac
     2701 ggccgctact ttaccgatct gatcggtgac aaagcgattg atttcatcga tcgacacgcc
     2761 gacgaggagt tcttcctctc cgtgccgttc aatgcgccgc actaccccat gcatgcaccg
     2821 cgggaatacc tcgatcggta tccggacctt ccaccggacc gacgcatcat ggccgcgatg
     2881 gtcagcgccg tcgatgacaa cgtggggcgt atcatggcgt cgctacagtc gcatgggctc
     2941 gcagacgaca cggttgtctt cttcagcagc gacaacggac cgagcacgga gacacgaaac
     3001 tggctcgatg gaactcccga caaatactac ggcggcagcg cgggaatctt ccgcggacat
     3061 aaggcca
//
LOCUS       DSCC01000104            2060 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036981, whole genome shotgun sequence.
ACCESSION   DSCC01000104 DSCC01000000
VERSION     DSCC01000104.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036981"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..456)
                     /gene="ilvN"
                     /locus_tag="ENN56_01215"
     CDS             complement(<1..456)
                     /gene="ilvN"
                     /locus_tag="ENN56_01215"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDS74136.1"
                     /translation="MSNRRHIISILVENHFGVLARVSSLIAGRGFNIDSLTVGETEDP
                     SLSRMTIVTRGDEMIIEQITKQLNRLVDVVKVTDLTETTHVERELVLAKISAPNSQVR
                     SEIIQVIDVFRAKVVDISANTMTVEVTGDNEKVEALIGMLEPFGIREMAR"
     gene            complement(456..>2060)
                     /gene="ilvB"
                     /locus_tag="ENN56_01220"
     CDS             complement(456..>2060)
                     /gene="ilvB"
                     /locus_tag="ENN56_01220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDS74137.1"
                     /translation="VVLKVYDEIYKRRRELHIIVPRHEQAGGHAADAYSRVTGRVGTL
                     FVTSGPGATNTVTALATAMMDSIPMVCFTGQVSRELIGSDAFQETDAIGITRPVVKHS
                     YMIERAEDIPRIIREAYYIARTGRPGPVLVDLPKNVTIETGTFHWPENVSMRGYKPTV
                     EGHPKSIQRLADAIAEAERPYLYIGGGVILSNASQELFELVEKTNIPVTTTLMALGAF
                     PGTHPLFVGMPGMHGSKVANICFQEADLVISIGARFDDRVTGKLGAFAPKAKIAHVDI
                     DPSSIKKNVKVDYPVVGDARWVLTELNKIVKPREPNGWNRLVADMKKTHWYKYRKDTN
                     VINPQYVIDMVYQMTKDRDTIITTEVGQMQMWAAQFYLFDKPRRWLTSGGLGTMGYGL
                     PAAIGAQIAAPDALVIDIGGDSSTQMNIQEFATIVQHNIPVKVANINNGYMGMVRQWQ
                     ELFYDRRYSSSCMGQIPNFERVAEAYGIKGITVREHAEVIPAIEQMISYPGPVFVDFW
                     VNPEEKVYPMVPPGAALHEMKDEEWA"
BASE COUNT          412 a          600 c          551 g          497 t
ORIGIN      
        1 ccgtgccatt tcgcgaatgc cgaacggttc gagcatccca atgagcgctt cgaccttctc
       61 gttgtctccg gtcacttcga ctgtcatggt gttcgcgcta atgtcaacca ctttcgcgcg
      121 aaacacgtcg atgacctgaa tgatctccga acggacttgt gaattcggcg cggaaatctt
      181 cgcgagcacc aactcacgct caacgtgtgt tgtctcggtg agatcggtca ctttcacgac
      241 gtcaacgagc cggtttaact gcttggtgat ctgttcgatg atcatctcgt cgccgcgggt
      301 gacgatcgtc atgcgcgaaa gagacggatc ttcggtctcg ccgacggtaa ggctgtcgat
      361 attgaatccg cgtccggcga tcagactact gacacgagcc aagacgccga aatggttttc
      421 gacaagtatg ctgatgatat gtcgtcgatt gctcattatg cccactcctc gtctttcatc
      481 tcgtgaagcg ccgcaccggg aggaaccatc ggatatactt tttcctctgg attgacccag
      541 aagtccacaa acaccggtcc cggatacgaa atcatctgct cgatcgcggg tatcacttct
      601 gcgtgttcgc gaacggtgat gcctttgatt ccatacgctt cggcgacccg ctcgaagttc
      661 ggaatctgtc ccatgcaact cgaggaatac cggcgatcgt agaagagctc ctgccactga
      721 cgcaccatac ccatgtagcc gttgttgata ttcgcgactt tcaccgggat gttgtgctgc
      781 acgatggtcg cgaactcctg aatattcatc tgcgtcgatg aatcgccacc gatgtcgata
      841 acaagcgcat caggagcggc gatctgcgca ccgatcgctg cgggaagtcc gtatcccatt
      901 gtgccgagtc caccggacgt gagccagcgc cgtggcttgt caaacaagta gaactgcgcc
      961 gcccacatct gcatctgacc gacttccgtt gtgataatgg tgtcgcggtc ttttgtcatc
     1021 tggtagacca tgtcgatgac atactgcgga ttaatgacgt tggtatcctt ccggtacttg
     1081 taccagtgtg tcttcttcat atccgcaacg agacgattcc agccgttcgg ttcacgcggt
     1141 ttgacgattt tgttcaactc tgtgagcacc cagcgtgcgt caccaacgac gggataatca
     1201 accttcacgt tcttcttaat cgatgacggg tcgatatcga cgtgggcgat tttcgctttc
     1261 ggcgcgaacg cccccagttt gcccgtcact cggtcgtcga aacgcgcacc aatcgagatc
     1321 acaagatccg cttcctggaa gcagatgttc gcgaccttgc ttccgtgcat tcccggcatg
     1381 ccaacgaaga gcggatgagt acccgggaaa gctccgagcg ccatgagcgt cgttgtcacg
     1441 ggaatgttcg tcttctcgac gagctcgaac agctcttgag acgcgtttga cagaatcact
     1501 ccgccaccaa tgtagagata cggacgttcc gcttcagcaa ttgcgtcggc aagccgctgt
     1561 atcgacttcg gatgcccttc gacggttggc ttgtaaccgc gcatgctcac gttttccggc
     1621 cagtggaacg tgcccgtttc aatggtgacg ttctttggga gatcgacgag gacgggtccg
     1681 ggacgacctg tccgcgcgat gtagtatgct tcacgaatga tccgtggtat gtcttccgcg
     1741 cgttcgatca tgtagctgtg cttcaccacc ggtcgcgtga taccaatcgc gtcggtttcc
     1801 tggaatgcat cggatccaat gagctcacgg ctcacctgtc cggtaaagca caccatcggg
     1861 atactgtcca tcatcgctgt cgcgagcgct gttacagtgt tcgtcgcgcc ggggccgctc
     1921 gtgacgaaca gcgtgccgac gcggccggta acgcgcgaat acgcgtctgc ggcgtggcca
     1981 cccgcctgtt catggcgcgg cacgataatg tgcaattccc gacgccgctt gtagatctcg
     2041 tcgtacacct tcagcacgac
//
LOCUS       DSCC01000105            1753 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046022, whole genome shotgun sequence.
ACCESSION   DSCC01000105 DSCC01000000
VERSION     DSCC01000105.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046022"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            180..>1753
                     /locus_tag="ENN56_01225"
     CDS             180..>1753
                     /locus_tag="ENN56_01225"
                     /inference="COORDINATES: protein motif:HMM:PF02901.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74138.1"
                     /translation="MIAVTKPESNIAETLESLGEPFAAGFMRKPDATMVERFAEGLRT
                     WARHLVLQPYRGELLYPSGPRVWNRDGMAVGWYYVGATGLDRGVFERKYADADEDGRS
                     ALSELERVWTLHRFPGGYTHSIPHYERVLAEGLDGFARRVADGLSRSTGDAERAGFYR
                     AMETTVDTARILHKRIVEEIERFNPLDAKSEENRAALLAAYARVPFAPADGFFDALVA
                     TNFIYYFDGPDNLGRIDQYLRPYFERSVARGEIDHDRAVDLIRAEWRNVDAASAWNVA
                     IGGISPQDGSDLSNELTVVCIEAARGMRRPNLALRLGPTTPDAVWDAALDTIASGCGL
                     PALYWDPNYYAAMDASGLPLPLEDRSEYAFGGCTELMVQGRSFVGSLDDSLNVPAVLE
                     HTVARILPTAPSFDAFYAAYRADLIEAIDEIVDRVNRWQSEKAEWQPQPIRSLLIDDC
                     VHRGVEYSAGGARYNWSVINVMGLANAIDSLAAIRSIVFDDGIASGREVVNALTSNFE
                     DAEELRLRLEHSPRYG"
BASE COUNT          333 a          504 c          558 g          358 t
ORIGIN      
        1 atgcataccg agtccgttga attcgctcat ggttccgctc cgttgtgata aaggttttcc
       61 catctacatg tcccgtgagg aatgataccc acgatacgcc gtcggtgaaa ccggaggttg
      121 cgttcgcgcc gccttcgtgt gttcgttttg gatggatact cgtataagga attttcggaa
      181 tgatcgccgt cacgaaacca gagtcgaata tcgctgagac actcgagtcg cttggggaac
      241 cattcgcggc cggtttcatg agaaaacccg acgcgacgat ggtcgagcga ttcgctgaag
      301 gtctccgaac gtgggcgcgg catctcgttc tgcaaccgta tcgcggtgag ctgctgtatc
      361 cgtcggggcc gcgagtctgg aatcgtgatg gcatggccgt cggatggtat tacgttggcg
      421 cgacggggct cgatcgcggt gtgttcgaac ggaaatacgc ggacgccgac gaggacggtc
      481 gctcggcact cagcgaactc gaacgtgtgt ggacgttgca ccgattcccc ggcgggtata
      541 cgcactcgat cccgcactat gagcgggttc tcgcggaggg actcgatggc ttcgcgcgcc
      601 gcgtagccga tggcctcagc cgttccacag gcgacgccga acgtgccgga ttctatcgcg
      661 ccatggaaac gacggtggat acggcacgga tactccacaa gcgtatcgtc gaggaaatcg
      721 aacggttcaa tccgttggac gccaaatccg aagaaaaccg cgccgcgctt ctcgccgcat
      781 atgctcgtgt accgttcgcg ccggcggacg ggttcttcga cgcgctcgtc gcaacgaact
      841 tcatctacta cttcgacgga ccggacaacc tcgggcgaat cgatcagtac ttgcgtccgt
      901 actttgaacg ctcggtggcg cgcggcgaga tcgaccacga tcgcgccgtc gatctgattc
      961 gtgcggaatg gcggaacgtc gatgcagcga gtgcgtggaa cgtcgcaata ggcggaatat
     1021 cgccgcagga cggtagcgac ctttcgaacg aactgacggt cgtatgcatc gaggctgcac
     1081 gcggaatgcg tcgcccgaat ctcgcgctcc ggctcggtcc gactacgcct gacgccgtat
     1141 gggatgcggc gctcgacacg atcgcatccg gctgcggact tcccgcgctc tactgggacc
     1201 cgaactacta cgccgcgatg gatgcgtcgg gtctcccatt gccgcttgag gaccggagtg
     1261 agtacgcgtt cggcggttgc accgagctga tggtgcaagg ccggtcgttt gtcggttcgc
     1321 tcgacgattc gctgaatgtc ccagcggtgc tcgagcacac cgttgcgcgg attcttccca
     1381 cggcgccgag cttcgacgca ttttacgctg cgtatcgcgc cgacttgatc gaagcgatcg
     1441 acgaaatcgt ggatcgagtc aatcggtggc aatcggagaa ggccgaatgg cagccgcaac
     1501 caatccgtag cctcctcatc gacgactgcg tccaccgtgg cgtggagtat tcggcggggg
     1561 gcgcgcggta taattggtcc gtcattaacg tgatggggct tgcgaacgca atcgattccc
     1621 ttgcggcgat acggtcgatt gtgttcgacg acgggatcgc gtcggggcga gaggtcgtca
     1681 acgcgctcac gtcgaacttc gaagacgcag aggagttgcg gctccggctt gaacactcgc
     1741 cgcggtatgg aaa
//
LOCUS       DSCC01000106            3405 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018847, whole genome shotgun sequence.
ACCESSION   DSCC01000106 DSCC01000000
VERSION     DSCC01000106.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018847"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            250..2286
                     /locus_tag="ENN56_01230"
     CDS             250..2286
                     /locus_tag="ENN56_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HDS74139.1"
                     /translation="MASEHTEPRAQDAKEEPKPKQPDTSSALADIDEPFVDGFTWNTI
                     AAMLFIGVVMMPGAILLGLVAGQSMGGAAEWVTIILFLEITKRSFVTMRRQELIIIYF
                     AAASLVAPGAHAIVGGQFATMIWNQYLIQSPQAEAIAMHIPTWVVPPKGSAPLIERTF
                     MSTLWLKPLGVVLATWFFFQINSLSFGYLVFRITSDIEKLPFPMAPVAAGGATALAES
                     STKSETWRWRIFSIGTILGLIWGTIYVVIPTLSSTFLTETVTILPIPFIDFTGNLRSV
                     LPTAVLAVNTEISGLLAGFVLPYWAVIGTFTAGMLKNFVINPVLYHNGIMERWEPGMS
                     LIPTTISVDFDFWLSAKIGIALFVFVVSIAAIVKSLMKGGQELDEKPVNEMDPEKVGP
                     GSGRGDVPIWLAALGFTVASVMLVFFIHWLVPEFPSWIVVIFAFVLTPMMSYISAKMT
                     GITGTTGAANFPYMREGSIYISGYQGVAIWFVPLPINDYGGWAQQFRVLQLTRTKFVS
                     YVKMIFANFIIVLIFSFIFWSFIWKMTTIPSSAYPYAQKMWPFHATMQVLWASSTLPG
                     GSNLMQGIVTIPKIASGFGIAAISWGAVMIGKGPLTLFYGLVSGFSGFPTGTYLMFAG
                     AMLNQFYFFPKFGQQRWRRYAPVLLAGYGCGMGLIAMTSIAVSLVMKSISSVVF"
     gene            complement(2358..2951)
                     /locus_tag="ENN56_01235"
     CDS             complement(2358..2951)
                     /locus_tag="ENN56_01235"
                     /inference="COORDINATES: protein motif:HMM:PF01986.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HDS74140.1"
                     /translation="MATGGSSVIVFNAEESAGVGGVYVLRITVSTRAHVVFGAFANGK
                     PICVEPGDYVYTGSARSSRGGTSLAARLIRHATRSGDLPPHRIRREIIDTFGQLGLWN
                     GRVPSGKKLFWNIDHLLDRLEAELVGVIALCTSDRREGEIARYLENEPHTSYLAKGLG
                     AHDIPGNTHLLRIDNVDAKWWEHLSETLVSFVNQETD"
     gene            complement(2953..>3405)
                     /locus_tag="ENN56_01240"
     CDS             complement(2953..>3405)
                     /locus_tag="ENN56_01240"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74141.1"
                     /translation="DDEGAPAQRVAIIENGVLANWLHGSYTANKAGEPNNGHGTRSGG
                     VSTSNINPALGERTAAELIAETKEGIYIDRGDLAPSMASGELSATVDFGFKIENGELV
                     YPVKNTMIGIGVWDMLNNLEAISSDYRAEPGAIMPTLRIAGVRVAGGE"
BASE COUNT          654 a          999 c         1007 g          745 t
ORIGIN      
        1 aatcaaccgc ggattcatga acgtcatgcg gaagcggttc ctcgtctggc gtaccatcac
       61 gccggatatc aaggacggct accgcgaacg cggacggcgc atattcgccg gcgaagacat
      121 gactgaggta ctggcgagtg gatcgggcga aacgactacc tgatcgaatc gggcagcgat
      181 atccgaaatg tctaatcggt gccgcggtcg gtagagagag ccgacgcgga tatgtcagga
      241 gatcaaccag tggccagtga acacacagag cctcgagcgc aggacgccaa ggaagagccg
      301 aagccgaagc agccggacac gtcatcggca ctggccgata tcgacgagcc gtttgttgac
      361 ggatttacgt ggaacaccat agcggcgatg ctgttcatcg gcgtcgtgat gatgccgggc
      421 gctatcctcc ttggcctcgt cgccggtcag agcatgggcg gcgcggcgga gtgggtgacg
      481 atcattctgt tcctcgaaat cacgaagcgt tcgttcgtga cgatgcggcg gcaggagctc
      541 atcatcatct actttgccgc ggcgagtctt gttgcgccgg gtgctcacgc gatcgtaggc
      601 ggtcagttcg cgacgatgat ctggaaccag tatctgatcc aatcgccgca agctgaagcc
      661 atcgccatgc atatcccgac gtgggtcgtt ccgccaaagg gcagtgcgcc gctcatcgag
      721 cggacgttca tgagcacgct ctggctcaag ccactcggcg tcgtgctcgc gacgtggttc
      781 ttcttccaga tcaactcgct ctcgttcggc tacctcgtat tccgcatcac gtcggatatt
      841 gaaaagctcc cgttccccat ggcgccggtt gccgccggtg gtgccacggc cctcgccgag
      901 tcctcaacga agtcggaaac gtggcgttgg cggatattct caatcggaac aatactcggt
      961 ctcatatggg gtacgattta cgtcgtcatt ccgacgttat ccagtacatt cctcaccgaa
     1021 acggtgacca tcctaccgat tccatttatc gattttaccg gcaatttacg ttccgtactg
     1081 ccgaccgccg tgctcgccgt gaacacggaa atcagcggct tgctcgccgg atttgtactg
     1141 ccgtactggg ccgtgatcgg gacgttcacc gccgggatgc tcaaaaactt cgttatcaac
     1201 ccggtcctct accacaacgg cataatggag cggtgggaac cgggtatgtc actgattccc
     1261 acgacgattt ccgtggactt cgacttctgg ctctcggcga agatcggtat cgcgctcttc
     1321 gtgttcgtgg tgagtatcgc cgcaatcgtg aagtcgctga tgaaaggcgg tcaggaactc
     1381 gacgaaaagc cggtgaacga aatggatccc gagaaagtcg gacccggaag tggacgcgga
     1441 gatgtaccaa tctggctcgc cgcactcgga ttcaccgtgg caagcgtgat gctcgttttc
     1501 ttcatccact ggctcgttcc cgaattcccc tcgtggatcg tcgtgatctt cgcgttcgtc
     1561 ctcacgccga tgatgagcta tatctcggcg aagatgaccg gtattaccgg taccacaggt
     1621 gcggcaaact tcccatacat gcgtgaaggc tcgatctaca tttcggggta ccagggcgtt
     1681 gccatctggt tcgtgccgtt gccgataaac gattatggcg gttgggcgca acagttccgc
     1741 gtgctccagc tcacgcgcac gaaattcgtc agctacgtga agatgatctt cgcgaatttc
     1801 atcatcgtgc tcatcttcag cttcattttc tggtcgttca tctggaagat gacgacgatc
     1861 ccctcgtccg cgtatccgta cgcgcagaag atgtggccgt tccacgcaac catgcaggtg
     1921 ctctgggcat ccagtacgct acccggcgga tcaaacctca tgcaaggtat cgtgaccatc
     1981 ccgaagatcg cgtcgggatt cggtattgcg gcaatttcct ggggtgcggt tatgatcggg
     2041 aaagggccgc tgacgctctt ttacggactg gtgagtggct tctccggctt cccgacggga
     2101 acgtatctca tgttcgccgg cgccatgctg aaccagttct acttcttccc gaaattcggc
     2161 caacagaggt ggcggcggta cgcaccggtg ctgctcgccg gatacgggtg cggcatggga
     2221 ctcattgcga tgacgtccat cgccgtgtca ctggtgatga agagtatctc gtcggtggtg
     2281 ttctaaaccg gacgattcag gaaatacgga aagcggggag tcgtgataaa cggctccccg
     2341 ctttccgtta cgagagttca gtcagtttcc tgattgacga aagatacgag cgtttcggag
     2401 agatgctccc accacttcgc atcgacgttg tcgatacgga ggaggtgcgt gtttccgggg
     2461 atatcgtgcg cgccgaggcc cttggcgagg taggacgtgt gcggctcgtt ttcgaggtac
     2521 cgcgcgattt cgccttcgcg ccggtccgac gtgcacagag cgatcactcc cacgagttcc
     2581 gcttcgagcc ggtcgaggag atggtcgatg ttccagaaga gcttcttccc ggacggtacg
     2641 cggccgttcc agagtccaag ctgaccgaag gtgtcgatga tctcgcgacg tattcggtgt
     2701 ggcggaagat caccgctgcg agtcgcgtgt cggatgagtc gcgcggcgag cgaggttccg
     2761 cctcgactcg agcgcgcaga gccggtgtac acgtaatctc ccggctcaac gcagatcggc
     2821 ttcccgttcg cgaacgcacc gaatacgacg tgtgcgcgcg tcgatacggt aattcgcagc
     2881 acgtacaccc caccgacacc ggcggattcc tcagcattga agacaatcac cgacgagccg
     2941 ccggtggcca tgtcattcac ctccggcgac gcggacaccg gcaatgcgaa gagtcggcat
     3001 gatcgcgccc ggttccgcgc ggtagtccga cgagatcgct tcgaggttat tgagcatgtc
     3061 ccacacgccg atgccgatca tcgtgttctt caccgggtac acgagttcgc cgttctcgat
     3121 cttgaatccg aagtctaccg tggcggaaag ctcacccgac gccatactcg gcgcgaggtc
     3181 cccgcggtcg atgtagatgc cttccttcgt ctccgcgatg agttccgccg ccgtccgttc
     3241 gccaagcgcg gggttgatgt tcgaggttga cacgccgcct gatcgcgtgc catgaccgtt
     3301 gttcggttcg cccgctttgt tcgccgtata gctgccgtgc agccagttcg ccagcacgcc
     3361 gttctcgatg atcgcgacgc gttgcgccgg cgctccttca tcgtc
//
LOCUS       DSCC01000107            2465 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029040, whole genome shotgun sequence.
ACCESSION   DSCC01000107 DSCC01000000
VERSION     DSCC01000107.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029040"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(257..1591)
                     /locus_tag="ENN56_01245"
     CDS             complement(257..1591)
                     /locus_tag="ENN56_01245"
                     /inference="COORDINATES: protein motif:HMM:PF05175.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDS74142.1"
                     /translation="MTKRDHRNNRNRKRTEQHSPRRRPKREEPRGMLSKVAIGEVSAT
                     LWISTGIEPSRAIKPYEHLLADTIAKLQPKRLLEIGSEVGAVGLAAAQSGWAERIVWV
                     QRDTVGLNAVQRTLAAQPLADYTAILGEGARDAASESFDAVVVHQQPSRKLTETLLRQ
                     AISVLEATGVIVLAGDIQLGARTAAAALAELCENVDVTEGGKTGLVAIGRNPHSSAAM
                     SEQAPRTTHEAEIHGTVFQWVTTPGVFSADDLDPATELLLDTVVLPHRGRILDMGCGP
                     GVIGLFAALHMPETDVTMIDTDIAAVRCAEEGIKRNCLTNARVVASDGIEAIERKRFD
                     VVLSNPPVHRAGRRDASLVSRFAEEAARAIGRKGRLWLVTAPTVPLQRLLGELFTEVA
                     VTEDFAQRFRVYDAIRRPRRHTRPDEVAVELNEFDDDIEFDEDDWGDDWDAE"
     gene            1809..>2465
                     /gene="folP"
                     /locus_tag="ENN56_01250"
     CDS             1809..>2465
                     /gene="folP"
                     /locus_tag="ENN56_01250"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDS74143.1"
                     /translation="MSTTLSRLLASHRTLVMGVVNITADSFSDGGAYLDPDAALAHAR
                     QLLEEGADILDVGGESTAPTSAGVDAEEESRRVLPVIEALAKDGVYVSVDTYKPEVAR
                     RALGAGARMVNDVTAFRGDRSLVDVVAEGDSDVCIMYAKDATPRTTRNQPVYEDVVIE
                     IAAFLKERTEYAEKHGVNRDRIIIDPGMGAFVSGEPGPRLEILHRLREFTELGYPVLV
                     G"
BASE COUNT          472 a          714 c          771 g          508 t
ORIGIN      
        1 accgaaaaca cgccgcgaaa gtcgacgagt cgaaaatcgg ctgtccgatg ctcgtcatcg
       61 gctcggaact cgaccgtatc gtgccggcgt cggtggtccg gagcgtggcg aagaaatacc
      121 gacgtgttgc cgaacaccga gagcttgagg gacattcgca ctgggtactc ggtgagcccg
      181 gatgggaacg tatcacggca caggttgtcg agtggcttga aacgacgtaa gacacatgct
      241 gccggggctt cgccgtttat tccgcgtccc agtcgtcgcc ccaatcgtcc tcatcgaact
      301 cgatatcatc gtcgaactcg ttcaactcga cggcgacttc gtccgggcgc gtatgcctac
      361 ggggacgccg tatcgcgtcg tatacgcgga aacgctgcgc gaaatcttcg gtaacggcca
      421 cctcggtaaa cagttctccg agcaatcgtt gcagcgggac ggtcggagcc gttaccagcc
      481 agagcctccc cttgcggccg atggcccgtg cggcttcctc ggcgaaccgg ctgacgaggc
      541 ttgcgtcccg acgtcccgcg cgatgcacag gcgggttgga gaggaccacg tcgaagcgct
      601 tccgttctat cgcttcaatg ccatcgctcg ccacgacacg ggcgttcgtg agacaattcc
      661 gcttgattcc ctcctcggcg caccgcacgg ccgcgatatc tgtatcaatc atcgttacgt
      721 cggtttccgg catgtgaagt gccgcgaaga gcccgatcac acccggaccg catcccatat
      781 cgagaattcg accgcgatgt ggcaacacaa ccgtatcgag gagcaattcc gttgcgggat
      841 cgaggtcgtc ggcactgaag acaccgggtg tcgtgaccca ttggaagact gttccgtgga
      901 tttcggcctc gtgcgtggtt ctcggtgcct gctcgctcat cgccgcggag gaatgcggat
      961 tcctaccgat ggcgaccagt ccggttttcc cgccttcggt gacatcgacg ttctcacaca
     1021 gttcagcgag tgccgcggcg gcggttcgcg caccgagctg aatatctccg gcaagaacga
     1081 taacgccggt tgcttcgagt acggaaatcg cttgacggag gagcgtctcg gtgagcttac
     1141 gtgaaggttg ctggtgaacg acgaccgcgt caaacgactc cgaggcggca tcgcgtgcgc
     1201 cttcaccaag aatcgccgtg taatcggcca acggctgtgc cgcaagcgtg cgctgaacgg
     1261 cgttcagccc gaccgtatcg cgctgtaccc aaacgattcg ctcagcccac ccggactgcg
     1321 cggcagcaag tccgactgcg ccgacctcac taccgatctc aaggagccgt ttgggttgga
     1381 gtttggcgat cgtatccgcg agaaggtgtt cgtacggctt tatggcacgc gacggctcaa
     1441 tgccggtgga gatccaaaga gtcgcggaaa cttcaccgat tgcgaccttc gacagcatgc
     1501 cgcgcggttc ttcacgcttc ggtcgtcgcc gtggtgaatg ttgttcggtc cgcttacggt
     1561 ttcggttgtt gcggtgatcg cgcttggtca tgcgataaag ctttccagtg aggatttgtc
     1621 cggaggatgt ttcattcaga aatgtcgcga gataccgagg tcgcggtaaa ggtccggcgc
     1681 ggagatcacc gtcgggaggt ggtgacacga tacgggttac cgtacctttt cgcggtgcgc
     1741 gttcgcggtg cgcgctcgcg gtacgcgctc gcggtacgcg aattcccgaa tatggagtct
     1801 gatttcctat gtctacgaca ctttcgcgct tgttggcatc gcaccgaacg ttggtgatgg
     1861 gtgtcgtgaa tattaccgcg gactcgtttt ccgacggtgg tgcgtatctt gaccccgacg
     1921 cggctctcgc tcacgcgcgg caactccttg aggaaggcgc ggatatactc gatgtgggcg
     1981 gcgagagcac agcgcctacg agcgcgggcg tcgatgccga ggaagagtca cgtcgggtac
     2041 tccctgtcat cgaagcgctt gcgaaggacg gcgtctatgt gtccgtcgat acgtacaagc
     2101 ccgaagtagc gcgacgagcg ctcggcgcgg gtgcccgcat ggtgaacgat gtgacggcat
     2161 ttcgtggcga caggagcctt gtagatgtcg tggcagaggg tgacagcgat gtctgtatca
     2221 tgtacgcgaa agacgcaacg ccgcgaacga cgcgaaatca gccggtctac gaggatgtgg
     2281 ttatcgaaat cgccgccttt ctcaaagaac gtaccgagta cgccgagaag cacggagtca
     2341 accgcgaccg aatcattatt gatcctggta tgggcgcgtt cgtcagcggg gagccgggtc
     2401 cgagactcga aatactccac cgtcttcgag agttcaccga gctggggtat cccgtgcttg
     2461 tcgga
//
LOCUS       DSCC01000108            1567 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053650, whole genome shotgun sequence.
ACCESSION   DSCC01000108 DSCC01000000
VERSION     DSCC01000108.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053650"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..354)
                     /locus_tag="ENN56_01255"
     CDS             complement(<1..354)
                     /locus_tag="ENN56_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_924769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDS74144.1"
                     /translation="MIQVRPSNERGKMNIDWLDTNYTFSFARYYDPKHMGFRSLRVIN
                     QDKVKPGTGFSTHPHNDMEIITYIIEGELEHRDNLGSRSVIRRGDIQRISAGTGVTHS
                     ETNPSNHETVHLLQIW"
     gene            complement(406..873)
                     /locus_tag="ENN56_01260"
     CDS             complement(406..873)
                     /locus_tag="ENN56_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_924768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDS74145.1"
                     /translation="MATRYRGTDDEVSALNAFIALQRAADALNAKLAPRIREYGLTDS
                     QFGVLEALYHLGPMHQNELGAKLLKSGGNMTLVIDNLEKRGFVRRVRSETDRRYITVH
                     LTGNGEELIARVFPEHAAAIADLMSVISPEERETLRRLARAVGTGSPTEVGRI"
     gene            complement(983..>1567)
                     /locus_tag="ENN56_01265"
     CDS             complement(983..>1567)
                     /locus_tag="ENN56_01265"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74146.1"
                     /translation="SPDKSIANTGADSIRRGLAAARTLGTDGVLVVPGTVTPEVPYDV
                     VWDRTKDRIGELVDEAKQTGVAICVENVWNKFHLSPIELRDYIDSFDSEFVRVYLDVG
                     NMLLYGYPEHWIRILGSRIKKVHFKDFRTSVGNGGGFVGLLEGDVNWPEVMKAFGEIG
                     YTGPASVELGPYKYHWEASIYVISLQMDYILGRR"
BASE COUNT          310 a          464 c          432 g          361 t
ORIGIN      
        1 ccaaatctga agcagatgca ccgtttcgtg attcgacgga ttcgtctcgc tgtgcgtcac
       61 cccggtgccg gcggaaatcc gctggatatc gccgcgccga atgaccgaac gcgatccgag
      121 gttgtcgcga tgttcgagct ctccctcgat aatgtaggtg atgatctcca tgtcgttatg
      181 cggatgagtg ctaaaacctg tgccgggttt caccttgtcc tggttgatga cacggaggga
      241 tcggaatccc atgtgtttcg ggtcgtaata gcgcgcgaat gaaaacgtat agtttgtgtc
      301 gagccagtcg atgttcatct tgccgcgctc gtttgacggt ctgacctgta tcattggaag
      361 tctccgttcc tgttgtattg agtcagtatc gatgtgtgat tgtgctcaga tccgcccgac
      421 ctccgtcgga gaacccgtac caaccgctcg cgcgagtcga cgaagcgtct cgcgttcttc
      481 cggcgagata accgacatca aatctgcaat cgccgcggca tgttcgggga acacccgcgc
      541 gatgagctct tcaccgttcc cggtgagatg caccgtgatg taccgccggt ccgtctcact
      601 gcgcactcgc cggacgaacc cgcgtttttc gaggttgtcg atcacgagcg tcatattccc
      661 accgcttttc agcagcttcg ctccgagctc gttctgatgc atcggtccga ggtgatatag
      721 cgcttcgagc acgccgaatt gactgtccgt cagcccgtac tcgcgaatac gcggtgcgag
      781 ctttgcattc aacgcgtccg ccgcgcgttg cagcgcgatg aacgcgttca gcgccgagac
      841 ctcatcgtcc gttccgcgat atcgtgtcgc cataacatct ccacattgaa taattcgata
      901 tcaaagtata taccgaaaaa aatcgccccg tcaagtcctt gtgacccgac agggcgatac
      961 gattcgatgt gtccgtgccg gattaccgcc ggccgaggat gtagtccatc tggagcgaga
     1021 tcacgtagat cgacgcttcc cagtggtact tatacggacc gagttcaacc gaggcggggc
     1081 ccgtatatcc gatttcgccg aacgccttca tgacttccgg ccagttcacg tcgccttcga
     1141 ggagcccgac gaaccctccg ccattgccga cagaggtgcg gaaatccttg aaatgcacct
     1201 tcttgatccg ggatccgagg atccgaatcc aatgttccgg atatccgtac agcagcatgt
     1261 tcccgacatc gagatagacg cggacgaact cgctgtcgaa gctgtcgatg tagtcgcgca
     1321 gctcaattgg gctgaggtgg aacttgttcc acacgttctc cacgcaaatc gccacaccgg
     1381 tttgttttgc ctcgtcgaca agctcgccga tccgatcttt cgtgcgatcc cacacgacgt
     1441 cataggggac ctctggagtc accgtaccgg gcacgacgag aacgccgtcg gtaccgagtg
     1501 tgcgcgcggc ggcgagaccg cgtcgaatcg aatcggcgcc ggtgttcgcg atgcttttat
     1561 ccggtga
//
LOCUS       DSCC01000109            2006 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038339, whole genome shotgun sequence.
ACCESSION   DSCC01000109 DSCC01000000
VERSION     DSCC01000109.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038339"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1002
                     /gene="ribF"
                     /locus_tag="ENN56_01270"
     CDS             <1..1002
                     /gene="ribF"
                     /locus_tag="ENN56_01270"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDS74147.1"
                     /translation="IGPLFVHAMMRHLVRSLEDIPLFENTAVTVGSFDGVHVGHRTIL
                     ERLRVSARARGLSSIAITFEPHHRIYFGREPRPFLLTDLDEKLALLGETSVEHTLVLP
                     FDGLLAGLSAREFLERVLIDRLGAKLFLVGHDQAIGRDQVRGPKNVARSAKGLDLDLE
                     TVPPIDAGGAPVSSTRIRTALTDGETTVALALLGNPYLLTGKVVHGDERGRQLGYPTA
                     NIALDEPYKLIPAPGVYIATADVLDTPTGDAQVRAMLYLGTRPTFDDSRTVVELFLMD
                     WTGDLYDTRLRVSIYARIRGDMRFDGASELVERMRRDEEITRTWFDERERCMMKLSM"
     gene            1123..1800
                     /locus_tag="ENN56_01275"
     CDS             1123..1800
                     /locus_tag="ENN56_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDS74148.1"
                     /translation="MVTVTYHGHNCVEFAGEAGKVIVDPFLSGNPLAEVGPDEIDVDA
                     ILLTHAHGDHLGDTVAIAKRLDVPVISTFEVVQWLERTYDVAGHAMHIGGSHRFDFGR
                     VRLVVAHHGNSTPDGISLGPPCGVVIDFDPTVVYHAGDTALTLDMKLLDGIIETIDFA
                     CLPIGGNFTMDIADAVHAADFIRPRMVMPIHYDTFDVIKADPKEFAKRVHCDCLVLRP
                     GESAEVV"
BASE COUNT          420 a          555 c          577 g          454 t
ORIGIN      
        1 attggaccgt tatttgtcca tgctatgatg cgtcatctcg ttcgcagtct cgaagatatc
       61 ccgctgtttg aaaacaccgc ggtaaccgtc ggatcgttcg acggtgtgca cgttggccat
      121 cgcacgatcc tcgagcgcct ccgtgtgtcg gcgcgggctc gcggactgtc ctcgatcgcg
      181 ataacgttcg agcctcacca ccggatatat ttcggtcgtg aaccgcgccc gttcctcctt
      241 accgatctcg acgaaaaact cgcccttctc ggcgaaactt cggtggagca tacactcgtc
      301 cttccatttg acgggcttct cgcgggattg tccgcgcggg agtttctcga gcgagtactg
      361 atcgaccgac tcggagcgaa gctgttcctt gtcggacacg atcaggcgat cgggcgagat
      421 caggtgcgcg gtccgaaaaa cgttgcacgt tcggcgaagg gtcttgacct cgatttggag
      481 accgttccgc ccatcgatgc gggaggagcg cctgtctcga gcacgcgcat tcgtaccgcg
      541 ctcacagacg gcgaaacgac ggtagcactc gctctcctcg gtaatccgta cctcctcacc
      601 ggaaaggtcg ttcacggtga cgaacgggga cggcagctgg ggtatcccac cgcgaatatc
      661 gcactcgatg aaccgtacaa gctgattccg gcacccggtg tgtacattgc tactgcggat
      721 gttctcgata cgccgaccgg tgacgctcag gtgcgcgcaa tgctctacct tggcacccga
      781 ccgacgtttg acgattcgcg gactgtggtg gaactgtttc tcatggattg gacgggtgac
      841 ctctacgata cacggctgcg agtttcgata tacgcgcgaa ttcgcggcga tatgagattt
      901 gacggggcgt ccgaattggt ggagcgaatg cgtcgtgacg aggaaatcac tcgtacatgg
      961 tttgacgaac gcgaacgatg tatgatgaag ctttcgatgt aataccgact gacatctttg
     1021 ggcgaggtaa cttgactaca ccgtgtcctg tcggtaaaat tgcttccgtt atcctcaaca
     1081 aaacgcgtcg caacgccgat tggattcatt ggagaagact gcatggttac ggtcacgtat
     1141 cacgggcaca actgtgttga gtttgcaggt gaagcgggta aggtaatcgt cgatccgttc
     1201 ctgagcggga atccacttgc ggaggtcggc ccggacgaaa tcgacgttga cgcaattctc
     1261 ctgactcacg cccatggcga tcatctcggc gatacggtag cgattgcaaa acggctcgat
     1321 gttccggtca tcagcacatt cgaagtcgtg caatggcttg agcggacgta tgacgtcgcc
     1381 ggccacgcga tgcacattgg cggctctcat cgttttgatt tcggacgtgt gcggctcgtc
     1441 gtggcacacc atgggaatag cacgcccgac gggataagtc tcggaccacc gtgcggggtc
     1501 gtaatagatt tcgatccgac ggtcgtttac cacgccggcg atacggcatt gacgctcgac
     1561 atgaagctcc tcgacggcat catcgaaaca atcgattttg cgtgcctgcc cattggtggt
     1621 aacttcacca tggatatcgc cgatgcggtt cacgccgcgg atttcatccg gccgcgcatg
     1681 gtaatgccga tccactacga tacattcgac gtgatcaaag ccgatccaaa ggaattcgcg
     1741 aagcgggtgc attgcgactg ccttgtgctc agaccgggcg aaagcgcgga agtcgtgtaa
     1801 cggtatcaat cggatggttc gaagaagccc gactgtacgc gatgcggtcg ggcttctttc
     1861 gtgtagcgga aatcctgaac accgattgac cccctccggt cgatactcta cattcccgct
     1921 tcaccagtat tcgcaacgta tagtggagat gtaacgttga gtcgaatcgc ggaaagcctc
     1981 ggcgaacgga atacgaatct tcgcac
//
LOCUS       DSCC01000110            2034 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037627, whole genome shotgun sequence.
ACCESSION   DSCC01000110 DSCC01000000
VERSION     DSCC01000110.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037627"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1000
                     /locus_tag="ENN56_01280"
     CDS             <1..1000
                     /locus_tag="ENN56_01280"
                     /inference="COORDINATES: protein motif:HMM:PF14579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74149.1"
                     /translation="ARREGGPFESIFDFCQRVDLKAVNKRMMESLIKSGAMDKFGERA
                     QLLEGTDALINWAVAEEADRSRGQVSLFEAVADAGIASDFDATPTLPNVAPLSPYDRL
                     QQEKELLGFYVSGHPLESHRRDVELFCTHTSDALEETSPETPVLMAGIIVAIRKITTK
                     AGKAMAMVTLEDFAGSYGAVVFPDTYAAARECLETDCIVMIRGTRDREEAKVLVDEVI
                     PIDQARQRLARAVDVRVLSRVLDDSGVDELKQMISINGGTLPVFLSVATKGHGTLRFR
                     IQGSSVNPTDEFIAKLEAHSAVEDVTLEAIELKREEQPNGRRRRQNGNRNAYAVGA"
     gene            1112..>2034
                     /locus_tag="ENN56_01285"
     CDS             1112..>2034
                     /locus_tag="ENN56_01285"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDS74150.1"
                     /translation="MPGNSFGHMFRITTFGESHGPAVGVVIDGCPPGIEITESDIQRD
                     LDRRRPGQSAITTARQETDAVEIVSGVFEGVTTGTPICMIVRNKDARSRDYSEIKDLF
                     RPGHADFTYLAKYGVRDYRGGGRASARETIGRVAAGALAKKLLERIGSRVVAYTCQVG
                     DVVLPTPDAPVTLEQIEANSVRCPNPAVAAQMEALIHTVRAERDSIGSAVELVAYGVP
                     TGLGEPVFDKLKADLAKALLSLPAVVGFEYGSGFRAVSMRGSEHNDAYEMCDGEVRTR
                     TNRHGGMLGGISSGEPIIMRVAIKPTSSIPKP"
BASE COUNT          469 a          545 c          606 g          414 t
ORIGIN      
        1 ggcgcggcgt gaaggcggtc cgttcgaatc gatattcgat ttctgccaac gtgtagatct
       61 caaggccgtg aacaagcgga tgatggaaag tttgatcaag tctggtgcga tggacaaatt
      121 cggcgagcgc gcgcagttac tcgaaggcac tgatgcgctc atcaattggg ccgttgccga
      181 agaggcggat cgttcgcgcg ggcaggtctc cttgttcgaa gccgtcgcgg atgctggaat
      241 cgcatccgat ttcgacgcga cgccaacact tccgaatgtt gcgccattgt cgccgtacga
      301 tcgactacag caggagaaag aactcctcgg attttacgtc agcgggcatc cgctcgaatc
      361 ccatcgtcgt gacgttgaat tgttctgcac gcacacgagc gatgcgctcg aagaaacatc
      421 tccggaaacg ccggtgctga tggcgggtat catcgtggct attcgaaaaa tcacgacgaa
      481 agccggtaaa gcgatggcaa tggttactct ggaggatttc gccggcagct acggagctgt
      541 ggtattcccc gatacgtatg ccgccgctcg agagtgtctc gaaactgatt gcatcgttat
      601 gatacgtggc acacgcgacc gagaagaggc gaaagtgctt gtcgacgagg tcattcccat
      661 cgaccaagct cgtcaacgac tggcgcgcgc tgttgatgtc cgggtacttt cgcgcgttct
      721 cgacgattcc ggcgtcgacg aattgaaaca gatgatatct atcaacggtg gtacgctgcc
      781 ggtctttcta tcggttgcga caaaaggtca cggcaccttg aggttccgca ttcaagggag
      841 tagcgtgaat ccgaccgacg aattcatcgc caagctcgaa gctcacagcg cggttgagga
      901 cgtaacactt gaggcgattg agctcaaacg ggaagaacaa ccaaacggtc gtcgccgcag
      961 acagaatgga aatcggaacg cgtatgccgt cggagcatag cgtcgcttcg gcttgtccag
     1021 acgattaaaa tgacgccatc tttcacaacg atcaatcggg tgttgtcaca tcccggaata
     1081 ctcgagcgaa cttacgaacg aaggttctcg tatgccgggt aatagcttcg gtcacatgtt
     1141 tcgcattaca accttcggcg aatcacacgg tcccgctgtt ggcgtagtca tcgacgggtg
     1201 tccgccgggt atcgaaatca ccgagtccga tatccagcga gatctcgacc gccgacgtcc
     1261 gggtcagagt gcgattacaa ccgcgcgaca agaaaccgac gcggtcgaaa tcgtcagcgg
     1321 cgttttcgaa ggggtgacaa ccgggacgcc gatctgcatg attgtgcgaa acaaggacgc
     1381 gcgaagccga gactacagtg agatcaaaga cctcttccga cccgggcacg cggacttcac
     1441 ctatcttgca aaatatggtg ttcgcgacta ccgtggcggt ggacgcgcgt cagcacgcga
     1501 gaccataggc cgagtcgctg ccggcgcgct tgcgaagaag ctgctcgaac gtatcggatc
     1561 gcgtgttgtc gcatacacct gtcaggttgg tgacgtcgta ctaccgacgc ctgacgcgcc
     1621 agtcactctc gagcagatcg aagcgaactc ggttcggtgt ccgaatccgg cagtagccgc
     1681 gcagatggaa gcactcatcc acacggtacg cgcggaacgc gatagtatag gaagtgctgt
     1741 cgagctcgtt gcatacggtg ttccaacggg actcggcgag ccggtattcg acaagctcaa
     1801 agccgatctc gcgaaagcgc ttctcagttt gccggctgtg gtcggattcg aatacggcag
     1861 cggatttcgc gcagtctcca tgcgcggctc agagcacaac gacgcgtatg aaatgtgcga
     1921 cggagaggtc cgaacgcgga cgaatcgaca tggcggtatg ctcggtggta tcagtagtgg
     1981 tgaaccgatc attatgcggg tggcaatcaa gccgacgtcg agtattccga agcc
//
LOCUS       DSCC01000111            3702 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016768, whole genome shotgun sequence.
ACCESSION   DSCC01000111 DSCC01000000
VERSION     DSCC01000111.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016768"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="ENN56_01290"
     CDS             complement(<1..1610)
                     /locus_tag="ENN56_01290"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03264.12,HMM:PF14537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74151.1"
                     /translation="MAAEEQNGKKWWRHWWVWVIGLPVALLVFTAFAAEYTSRSSFCV
                     TCHYMVPFYESWQESEHHDIECVQCHFPPGMAGKIKGKVNGLYQVAAYVTQAYRRSRP
                     WAEIEDASCMRSGCHDSGILSDTNIVIFKNVAFTHGSHLNEPRRDRQLRCTSCHSQIV
                     QGEHMLVTTSTCALCHLWHGGVLADDNPRQTESGECRLCHTMTAETRPQNHPNVDKLD
                     VACVSCHGKTTIGDGAVSLDRCYQCHFERDRLDRYDEHVLLHQIHITDNKIDCLNCHT
                     RIQHKTVIAQEVAQLDCQGCHPNLHEPQHTLFTGRGAHMVPNMPNAMFSRGIQCQSCH
                     VYHREVSFPDEAELGETVYADEDACAQCHGRGFTGLVEQWNSFIYRRLSDLEGIDRRV
                     RTRARPITDARVASLLDSVSYNMRVVRDGHPVHNAVYAKAILDSTYSLLRTVANSVEP
                     RISLPPFDTNVGIDVPGACRTCHFAIETVNVRAFGEYPFSHNIHAVQQQLPCEMCHSN
                     TATHGEHIVTPSDCTDCHHTTEKPNQCAV"
     gene            complement(1750..>3702)
                     /locus_tag="ENN56_01295"
     CDS             complement(1750..>3702)
                     /locus_tag="ENN56_01295"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01292.18,HMM:PF02085.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74152.1"
                     /translation="VTLLLTLCASVFVVPIANAQLSSQDCLDCHGDADIMEGMESYLV
                     QPGALDESVHAFFECTDCHNVTDIPHDDLGPPTCASCHDMAQEEFDESVHGMAFAKGE
                     PEAPGCGSCHGGHTIRYVDDPTSLVAPRKQPATCGTCHSNPDVVRKYYMSAANPSEAY
                     ERSAHYAALMREGDDAPEKDPPTCSTCHGAHAVQRASNPDSPVYAAKVGELCGRCHTE
                     IYDIYAGSVHGQAHSAGNRNAPTCVSCHGEHNIVTTADGRDIDEIIRLSSETCATCHA
                     DQQLIRNMGLSALRVTSYRESYHGLAGQAGSEQIARCASCHGVHNIYRARDPRSLVHE
                     NNLVFTCGNCHEGINDRFAQIRVHALYTTETTTPAGIVRVTYIFLIVSILGGMMIHNF
                     VIYSRYIREKYRAEKRLRRLRRFPRFQLTQHIILILSFMTLAITGFALKYGGSFWVEW
                     LQSVGFDEATRRLTHRFAAVVLMVQSVVQMVWFVITREGRRDVRSLIPTYQDVIDAIQ
                     NMRYHLFHAEHPPRYGRFDYVEKAEYLALIWGTAVMAITGLVLWFPELATRFWPAWTI
                     EVATMIHYYEAILATAAIFVWHWFFVIYHPREFPMRLTWLTGAMTEHDYRHHHPREYE
                     ELRNNPDVVLPPQHDTQDRRESNETS"
BASE COUNT          724 a         1068 c         1133 g          777 t
ORIGIN      
        1 accgcgcact ggtttggttt ctcggtggtg tggtgacagt cggtgcagtc ggagggagtc
       61 acgatatgtt cgccgtgcgt cgcggtattc gagtgacaca tctcgcaagg tagttgctgc
      121 tggactgcgt ggatattgtg cgagaacgga tactcaccga acgcacgcac gttcaccgtc
      181 tcgatagcga agtgacatgt ccggcaggct cccggtacgt caatgccgac attcgtgtcg
      241 aaaggcggca aggatatccg cggttcgacg ctgtttgcca cggtacgaag gagactgtat
      301 gtgctgtcga ggatcgcttt cgcgtatacc gcgttgtgca ccgggtgccc atcgcgaacc
      361 acccgcatgt tatacgacac cgaatcgagc agcgaggcga ctcgtgcgtc ggtgatcggg
      421 cgggcacgcg tacggacgcg ccgatcgata ccttcgagat cggaaagcct acgatagatg
      481 aacgagttcc actgctcgac gagtccggtg aaaccgcgtc cgtgacactg tgcgcaggca
      541 tcctcatcgg cgtacaccgt ttcgccgagt tccgcctcat cagggaagct cacctcgcga
      601 tgatacacgt gacagctctg acactgaatc ccgcgagaga acatcgcgtt gggcatattc
      661 ggcaccatgt gcgcgccgcg accggtgaac aacgtatgtt gtggttcgtg gaggttcggg
      721 tggcacccct gacagtcgag ctgcgcgact tcttgagcaa tgaccgtctt gtgttggatt
      781 cgcgtgtgac agttcagaca gtcgatcttg ttgtcggtga tatgtatctg gtggagcagt
      841 acgtgctcat cgtaccggtc gagtcggtcg cgttcgaagt gacactggta gcatcggtcg
      901 agcgatacgg cgccgtcgcc gatggtcgtt ttcccgtgac aggaaacgca ggcaacatcg
      961 agtttgtcga cgtttgggtg attctgcggg cgcgtctccg ccgtcatggt gtggcagagg
     1021 cggcactcgc ccgattcggt ctgtcgcggg ttgtcatcag ccagtacgcc gccgtgccaa
     1081 aggtgacaca gcgcgcacgt ggaggtggtc acgagcatgt gctcgccttg aacgatctgc
     1141 gagtgacatg atgtacagcg gagctgacga tcgcgacgcg gttcgttgag atgggagccg
     1201 tgggtgaacg cgacgttctt gaagatgaca atgttcgtgt cggacaggat accggaatcg
     1261 tgacagcctg accgcatgca cgatgcgtct tcgatttccg cccacggacg cgatcgtcga
     1321 tacgcctgag tcacgtacgc ggcgacttgg tagagtccgt tgaccttacc tttaatcttc
     1381 cccgccatgc ccggcgggaa atggcactga acgcactcga tatcgtggtg ttcggattcc
     1441 tgccacgact cgtagaacgg caccatgtag tggcatgtta cgcagaacga cgagcgcgac
     1501 gtgtattccg cggcgaaggc cgtaaacacg agcagagcga ctggcaaccc aatcacccat
     1561 acccaccaat gccgccacca tttcttcccg ttctgctctt cggcggccat tgcgccctcc
     1621 tcgtcgccgg atatcgacgc gttccttctt gtcacaatac gatcacacgc agatgcgcga
     1681 ttccgttgta cccttacgcg taccgcctcg aatacgaagt gctaccggtg acatgaccgt
     1741 tttccatgcc tacgatgtct cgttcgattc ccgtcgatcc tgtgtgtcat gctgcggggg
     1801 gaggacgaca tcaggattgt tccgcaactc ctcatactcg cgtggatgat gatgccgata
     1861 gtcgtgttcg gtcatcgcac cggtgagcca tgtcagtcgc atcgggaact cgcgcggatg
     1921 gtagatcacg aagaaccagt gccacacgaa aatcgccgcc gtcgcaagga tggcttcgta
     1981 gtagtgaatc atcgtcgcga cctcgatggt ccacgcgggc cagaaacgtg tcgccagctc
     2041 cgggaaccag agcacgagac ccgtaatcgc catcaccgcg gtaccccaga taagcgcgag
     2101 gtattccgcc ttttccacgt aatcgaagcg gccgtaacgc ggtggatgct ccgcgtggaa
     2161 gaggtggtag cgcatgttct ggattgcgtc gatgacatcc tgataggtcg ggatcaacga
     2221 gcgtacgtcg cgacgcccct cgcgcgtgat cacgaaccac accatctgaa cgaccgactg
     2281 caccatgagc acaacagcag cgaatcgatg ggtcaaccgc cgcgtcgcct cgtcgaagcc
     2341 gacgctctgt agccattcga cccagaacga acctccatac ttcaacgcaa acccggtaat
     2401 cgcaagggtc atgaagctca agatgaggat gatgtgctgt gtgagctgga agcgcgggaa
     2461 ccgacgcagg cgacgcagcc gcttttcggc gcgatacttc tcgcggatgt agcgcgaata
     2521 gatcacgaag ttatggatca tcataccgcc gaggatcgat acgatcagga aaatgtacgt
     2581 gactcgcacg ataccggcgg gcgtcgtcgt ttccgttgtg tagagcgcgt ggacgcgtat
     2641 ctgcgcgaaa cgatcgttga ttccctcgtg acagtttccg cacgtgaaaa cgaggttgtt
     2701 ctcgtgcacc aaactgcgcg ggtcgcgcgc tcggtagatg ttatgcacgc cgtgacaact
     2761 cgcgcaacgc gcgatttgct ctgaaccggc ttgtcccgcg agaccgtggt aactctcgcg
     2821 gtagctcgta acccggagtg cggagagacc catgttacgg atcagttgct gatcggcgtg
     2881 acacgtggcg cacgtctcgc tcgaaaggcg gatgatctcg tcgatgtcgc ggccgtccgc
     2941 ggtggttacg atattgtgtt cgccgtgaca cgagacgcag gtcggtgcgt tccgattgcc
     3001 cgctgagtgc gcctgcccgt gaacactgcc tgcgtagatg tcgtagatct cggtatggca
     3061 cctaccacag agctcgccaa cctttgccgc ataaaccggc gaatctggat tcgatgcgcg
     3121 ttggaccgcg tgcgctccgt ggcaggtgct gcacgtcggt gggtcctttt ccggcgcgtc
     3181 gtcgccttcg cgcatgagcg ctgcgtagtg cgcgctccgc tcatacgcct cgctcgggtt
     3241 cgctgcggac atatagtact tccgcacgac atcgggattc gagtggcacg taccgcacgt
     3301 cgccggttgt ttacgcggcg cgacaagtga cgtcgggtcg tcgacgtacc ggatagtgtg
     3361 cccgccgtga cagcttccgc agccgggcgc ttccggctcg cctttggcga atgccatacc
     3421 gtgaacggac tcgtcgaatt cctcttgcgc catatcgtgg caggatgcac acgtcggcgg
     3481 accgaggtcg tcatgcggaa tgtcggttac gttgtggcag tcggtgcact cgaagaacgc
     3541 gtggaccgat tcgtcaaggg cacccggttg aacgaggtag ctctccatgc cctccatgat
     3601 gtccgcatcc ccgtggcaat cgaggcagtc ctgcgacgag agttgcgcgt tcgcaatcgg
     3661 aacgacgaaa accgacgcgc agagcgtcag gaggagcgtg ac
//
LOCUS       DSCC01000112            2417 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029780, whole genome shotgun sequence.
ACCESSION   DSCC01000112 DSCC01000000
VERSION     DSCC01000112.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029780"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1961
                     /gene="dnaE2"
                     /locus_tag="ENN56_01300"
     CDS             <1..1961
                     /gene="dnaE2"
                     /locus_tag="ENN56_01300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743853.1"
                     /note="DNA polymerase involved in damage-induced
                     mutagenesis and translesion synthesis. It is not the major
                     replicative DNA polymerase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HDS74153.1"
                     /translation="IDLDIMHTRREEVIQWVYEKYGRSHAAMVANVIRYRPKSAVRDV
                     GKAFGLPITTIDRLAKLLSSYGGITEEAFHAAGIDLHIPVHRHLFQLANEILKTPRHL
                     SIHPGGFLLGHEPVHDLVPIENATMENRTVVQWDKYDVEELGLFKVDLLGLGGLTLID
                     TTLRLLKAHRDIDLTMATIPPDDTATFDMIGRAETIGVFQIESRAQMATLPRMKPRSY
                     YDLVIEIALIRPGPITGDMVNPYLRRRDGDEPVTYPHPCLESVLKKTLGVPLFQEQVM
                     RIAIEAADYTPGEADQLRRDMAAWKLHGSIDKHRERLLSRMVAKGIDEKYAERICAQI
                     QGFGEYGFPESHSASFALIAYVTSWLRCHYIDEFICGLLNAQPMGFYSVDTIIHDAQR
                     RGVVIRPVDVLYSMEDCTLEPLETGRHAVRIGLRYVKNLLNSAAERMVSARASGQFTS
                     ISNVARRAKLDQRSLAALATAGAFESLSANRRDAYWCASGTSYDDVLPLGIAEPDPMP
                     EFEPLAPHEVTVWDYRSVGHTTRPHPLANLRVQLKQQGIPDAATVVQMKNGAWTRYAG
                     MVICRQRPATAKGVMFATLEDESGTVNIILWSHVLERQSVIAKTASFLGVSGQISSEK
                     GVIHIVAKKLWVPRFDRTVGSVRSRDFR"
BASE COUNT          597 a          652 c          626 g          542 t
ORIGIN      
        1 atatcgatct cgacatcatg catacgcggc gagaagaggt catacagtgg gtctatgaaa
       61 agtacggacg ctcccacgcg gcgatggttg cgaatgttat ccggtatcga ccaaaatctg
      121 ccgtgcgaga cgtgggaaaa gcgttcggtc taccgattac gacgattgat cgactcgcga
      181 agttactctc ttcatatgga ggtattactg aagaagcttt tcacgcagcg gggatagacc
      241 tgcacatacc tgttcatcgg catttgtttc aacttgcgaa cgaaatcctc aaaacaccac
      301 ggcatctctc gatccacccg ggtggattcc tcctcggaca cgaaccagtt cacgatcttg
      361 tcccaataga aaacgcgacc atggaaaacc gaacggtggt gcaatgggat aagtatgacg
      421 tcgaagagct gggtctgttc aaagtcgatc tactgggatt gggcggtttg acacttatcg
      481 acacgacact gcgattattg aaggcacacc gtgacatcga ccttacgatg gcaacgatac
      541 cacctgacga cactgcaacc ttcgatatga ttggtcgtgc ggaaaccatt ggcgtctttc
      601 agatcgaaag tcgcgcgcag atggcaacac tgccgcgaat gaagccgcgt agctattacg
      661 atctcgtaat cgaaatcgcc ttgattcgcc ccggtccgat taccggagac atggtcaatc
      721 cgtatctgcg acgacgtgac ggagatgaac ccgtaacgta tccgcaccca tgccttgagt
      781 cggtgctaaa gaaaacactc ggcgttcccc tgtttcagga acaggtaatg cgaatcgcca
      841 tcgaagccgc tgactatact ccgggggaag ccgatcaact acgacgtgac atggctgcgt
      901 ggaaactcca tggtagtatt gacaaacatc gtgagcgtct cctttcgcga atggtcgcga
      961 aaggtatcga cgagaaatac gcagagcgta tctgtgcaca aatccaagga ttcggcgaat
     1021 acgggtttcc cgagagccac tctgcatcat ttgcactgat tgcatatgta acgtcgtggc
     1081 tcagatgcca ctacatcgat gagttcattt gtggccttct caatgcacaa ccaatgggct
     1141 tttactcggt agacaccatc atacatgacg cacaacgccg cggtgtagtg attcgtccgg
     1201 tagatgtttt gtacagtatg gaagattgca cattggagcc gcttgaaacc ggtagacatg
     1261 cggtgcgtat cgggctgcgg tacgtaaaaa acttactcaa ctcggccgcg gaacgtatgg
     1321 tatctgctcg tgcaagcgga cagtttacct cgatatccaa tgtcgctcga cgagcgaagc
     1381 tcgaccagcg atcacttgcc gcattggcca ccgccggagc atttgaatct ctcagcgcaa
     1441 accgacggga cgcgtactgg tgtgcgagtg gaacttcgta cgatgatgtg ttaccacttg
     1501 gtatcgccga acccgatcca atgcctgaat tcgaacctct cgctccgcac gaagtcactg
     1561 tgtgggatta ccgcagcgtt gggcatacca cgcgcccgca tccgctcgcg aacttacggg
     1621 tgcagttgaa acagcaggga atccctgatg ctgcaaccgt cgtgcagatg aagaacggcg
     1681 cgtggacgcg ttacgcggga atggtgatct gccggcaacg acctgcgact gcgaagggtg
     1741 taatgttcgc aacattggaa gacgaaagcg gcaccgtgaa tatcattctc tggtcgcatg
     1801 tattggaacg gcagagtgtc attgcaaaaa cggcatcgtt tctcggtgta tcggggcaaa
     1861 tttcatcgga gaagggtgtc atacatatcg tcgcgaagaa actgtgggta ccgagattcg
     1921 atcggaccgt aggaagcgtt cggagcagag actttcggta gtcaccacga gtcgattccg
     1981 tcgtacaccg gtacggacca aacccatctc gtgaaactac gtgagcaggc gttgccgcgt
     2041 acaatccgcg catccatccg caacgcgaaa cagaaacaaa acctacaccg cgacttctca
     2101 ttttctctac gtgttcccga tcctttcccc tcctttctca ctcaatcgcc ttacagctct
     2161 gcaccaaaat actcggttat gataccgatt aggtgcgcgg tctgattcac gagttcgtca
     2221 agcggcatga cttccagatc ggaatgcgca cctactgcgc gagttccgca gtacacggcc
     2281 ggtatgcccg cttcacccgc caaaatggat acgtcaccaa ccgacatgaa cgcgcttcgt
     2341 ggcatctcac gtccttcgat acgtcggaaa ctgtcctgat acgcgcgcac gactttcgcg
     2401 tcttctggaa cttcata
//
LOCUS       DSCC01000113            1869 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042251, whole genome shotgun sequence.
ACCESSION   DSCC01000113 DSCC01000000
VERSION     DSCC01000113.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042251"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            34..1833
                     /locus_tag="ENN56_01305"
     CDS             34..1833
                     /locus_tag="ENN56_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74154.1"
                     /translation="MGLMYGVWIPANDPPMARHRPEWNRGWREYVCPSIPAAREAILR
                     SRAEYVSRIPHLDILFVPSADDGGCWCADCVPWSETYLGLVRQQWEIARRYHPNVRVM
                     LSNQALTHDENLVLCDRLRELEDTAWIAGVAFAPGSNEVFRHARLTEEWSSFDRPDFG
                     DEVLSVKTFRRNLPASIDLYLYPDITHSLRCQYPIWEIDPLVGHAYHRDPVFFRPTHF
                     HRVYGLTAPFADGSFPYSEGMYDNLNQIVWLELGNDPQKAPHQIVYDYWRWYVGDAAA
                     PFAAAATDSLEESWRSDFLTTTEIDRAARNTFELCQHLPRNRERGNWRADILRLRVLT
                     DEWLRERIDQAEGIEKRIVTQLKEVDSDTDAQSLKRALHHAAEIARGGIAEMGSEPHD
                     PEIPHLAQRVGAEGLDIPAIERIGEVVGNLRWITRTLDDAIASIEIESMRRARIDVID
                     YERGTVYIDCGHPRRDPLGGPRLNGRTFITLDTEDLDVRSSQISFAMTYADDEPLSYR
                     LTGLDRTREYCIHVTYYLPPYLRRWCQSGEQRLEAGGIPLHGPLRLQSDRPTHHEFPL
                     PKEAYPTDETVLRFIPTGESRAAMVSEIWLRER"
BASE COUNT          403 a          525 c          554 g          387 t
ORIGIN      
        1 atacaagccc gtgtatcgga gcttgtccac gatatggggc tcatgtacgg cgtgtggata
       61 ccggcaaatg atcccccaat ggcgcgacat cggccggaat ggaatcgcgg ttggcgggag
      121 tatgtatgcc cgtcgattcc cgctgcacgc gaggcaattc tccgcagtcg agccgagtac
      181 gtctcgcgaa tcccgcacct cgatatcctc ttcgtcccgt ctgcggacga cggcggatgt
      241 tggtgtgcgg attgcgtccc gtggtctgag acgtatctcg gactcgtgcg ccaacagtgg
      301 gagatcgcgc gacggtacca tccgaacgtg cgtgtgatgc tttcgaatca ggcgctgacg
      361 cacgacgaga atctcgtgct ctgcgatcgg cttcgcgagt tggaggatac ggcgtggata
      421 gccggcgtcg cgtttgctcc gggatcgaac gaagtattcc gccacgctcg gttgactgag
      481 gaatggtcgt cgttcgatcg gcccgatttc ggcgatgagg ttctgagtgt aaaaacgttt
      541 cgtcggaacc ttccggcgag catcgatctc tacctttacc cagacatcac acactcgcta
      601 cggtgtcaat acccgatctg ggaaatcgat cctctcgtcg ggcatgccta ccatcgcgat
      661 cccgtgttct tccgtcccac gcacttccat cgcgtgtacg ggctgaccgc accgtttgct
      721 gatggatcgt tcccctattc cgaggggatg tatgataact tgaaccagat cgtctggctc
      781 gagttgggaa acgatccgca gaaggcacct caccaaatcg tttacgatta ctggcgctgg
      841 tacgtcggcg acgcagcggc cccattcgcc gcagccgcga cggattcgct ggaagagtcc
      901 tggcggagcg acttccttac gacgacggaa atcgatcgcg cggcgcggaa tacctttgag
      961 ttatgtcagc atcttccgcg aaaccgtgaa cggggaaact ggcgagcgga cattcttaga
     1021 ctgcgcgtac tcaccgacga atggcttcgt gaacggatcg atcaagcgga gggaatcgag
     1081 aaacggattg ttacgcagtt gaaggaagtc gattccgaca ccgatgcgca gagcctcaaa
     1141 cgagcattgc atcacgccgc tgaaattgcg cgcggcggaa tcgcggaaat gggaagcgaa
     1201 ccgcatgacc cggagattcc gcatctcgcg caacgcgtcg gcgcggaagg ccttgatata
     1261 ccagcgattg agcggattgg cgaggtggta ggaaacctcc gctggatcac gcgaacactc
     1321 gatgacgcga ttgcttcgat cgaaattgaa tcaatgcggc gcgcgcggat cgatgtcatc
     1381 gactacgagc gcggcacggt ctatatcgac tgcggacatc cgcgacgcga cccactcgga
     1441 ggcccacgac tgaatggtcg gacgtttatc acgctggaca cagaagattt ggacgtaaga
     1501 tcgtcgcaga tctctttcgc catgacgtac gctgacgacg agccgctgag ttaccgtttg
     1561 acgggactcg atcggacgcg cgagtactgt atacacgtaa cctactacct accgccgtac
     1621 cttcggcgat ggtgtcaatc cggtgaacaa cgactcgaag ccggcggaat tccactgcac
     1681 ggaccgcttc ggcttcagag tgatcgaccc acgcatcacg agttcccatt accgaaagaa
     1741 gcgtatccta ctgacgaaac ggtactgcgt ttcattccga cgggtgaaag tcgtgccgcc
     1801 atggtgagtg aaatctggct acgggaacgg tgaaaattga gtctctcgct tgcacagacg
     1861 gacgattgg
//
LOCUS       DSCC01000114            3450 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018507, whole genome shotgun sequence.
ACCESSION   DSCC01000114 DSCC01000000
VERSION     DSCC01000114.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018507"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(35..1510)
                     /locus_tag="ENN56_01310"
     CDS             complement(35..1510)
                     /locus_tag="ENN56_01310"
                     /inference="COORDINATES: protein motif:HMM:PF00924.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDS74155.1"
                     /translation="MALPRVDQWEQSWIRGLRIGGWEVLSAGQIADLLMIALEGLRLA
                     IGFMFFIWIAGHILDLVPFIEGWNYTLLLTSLLGATLLTVVMIVVIRAIIMLYHTFIA
                     RVREWKGTLIKTVRVKRVELVTQERLGELLEGAIRIFYLVLLISLVYVWLTSVFSIFP
                     FTEHWGEQLAAYVLNPTTSIIMAFVNYLPNLFTIIVIVIVARYLLKFVRWFFREVGRG
                     NIPLGGFYTDWAAPTYNIVRFLILAFVAIIMFPYLPGSDSPAFGGISVFLGLMLSLGS
                     ASAIANVISGVVLTYMRAFRVGDRVKIADTIGDVTEKTLLVTRVRTVKNVDITIPNAM
                     VLGSHITNFSSSAVESGLVLHTTVTIGYDVPWRKVHELLVEAALRTNGVHKEPEPFVL
                     QTGLQDYYPAYELNVYTEEPNRMARIYSELHGNIQDVFNEAGVEILSPAYGAVRDGNA
                     STIPNDYLPKDYDAPSFKVRTVTDLLTQHQTVKREGGERDT"
     gene            complement(1435..2391)
                     /locus_tag="ENN56_01315"
     CDS             complement(1435..2391)
                     /locus_tag="ENN56_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74156.1"
                     /translation="MSRTAVVTALVFAALLVFGNAYAQRDTLHSNQSPTDADSLAVGT
                     HVITDTSVVVTSDTVVVKADFTILSEAARWWDAFSPLDLSLVFLLLIATVVIWRGISI
                     LRPRVRVKLDELARTGRIKPLIAWNRILVRARTWTTIADVTLRVLRFFIILTLCYLAM
                     ITVVGVARPENAAPFGEVVRGLLIAAFALFLARSANRSWPTLYERARSWEGTHIRAIR
                     LGRFVLATVAEATHMVVNILVTLRILFVLIVTYSILSYLLGVFAIDEESIIANAVGGL
                     FVAVFVVTIYVLLNRLFPWLYRAWISGSSRGYAACGSAGGRF"
     gene            complement(2615..3403)
                     /locus_tag="ENN56_01320"
     CDS             complement(2615..3403)
                     /locus_tag="ENN56_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74157.1"
                     /translation="MKSISRGIIAGAILVVGLSIGCSTVDAQTRPSRNRNMVDRFHRV
                     AIRFNTFAAVPNDPRGRIAYGTERGNVYVLEFENGYYRSIWSSPSMATRIKEVQFADL
                     DVDGRYALIAYNTRGQLAIWNIDDFSVRWESPETHFLSIEALTTGQVDHDPQLELLFM
                     SEGRLFIYDGKHFVEEWRSQQIFDATDIVVGDVDGDDEPEIILNTGFVLDAFTRSLKW
                     ESLDEFGEILELVDIDGDGKLELISGSVGATKVWDIDMRREKWE"
BASE COUNT          825 a         1008 c          951 g          666 t
ORIGIN      
        1 caagcgcgaa cgcgagcgta gcgaagggtt cgcgctacgt gtcgcgttcc ccaccctccc
       61 gtttcaccgt ctgatgctgc gtgagcagat cggttaccgt gcgtaccttg aacgacggcg
      121 cgtcatagtc cttcgggagg tagtcgttcg gaatcgtact cgcgtttcca tcgcgaaccg
      181 cgccgtacgc cgggctgagg atctcgacac cggcctcgtt aaagacatct tgtatatttc
      241 cgtggagctc cgagtaaatc cgcgccatac ggttcggttc ttccgtatac acgttcagct
      301 cgtatgcggg ataatagtcc tgcaatcccg tctggagcac gaacggttcc ggctccttgt
      361 gcacgccatt cgtgcgtaat gccgcctcga ccagcagttc atgcaccttt cgccacggca
      421 catcataccc gatcgtcacc gtagtatgga gcacaagacc cgattccacc gccgacgagc
      481 tgaagttcgt gatgtgactg ccaagtacca tcgcgttcgg aatcgtgata tcgacgttct
      541 tcactgtccg aacgcgcgtg acgagtaacg tcttctccgt cacgtcgccg attgtatcag
      601 cgatcttcac ccgatcaccg actcggaacg cacgcatgta cgtgagcacg acaccggaga
      661 taacattcgc gattgccgat gccgaaccga gagacagcat gagtccaagg aagactgaaa
      721 tcccgccgaa tgcgggcgaa tcgcttcccg gtaggtacgg gaacatgata atcgcgacga
      781 acgccaggat aaggaatcga acaatattgt acgtcggcgc cgcccagtcc gtgtagaaac
      841 caccgagcgg aatgttacca cggccgacct cccggaaaaa ccaacgcaca aacttgagga
      901 gataccgcgc gacgatgacg ataacgatga tcgtaaacag attcggcagg tagttgacaa
      961 acgccatgat gatggacgtt gtgggattca gcacgtacgc ggcgagttgc tcgccccaat
     1021 gctcggtgaa tggaaaaatg ctgaacacgc tcgtaagcca tacatacacg agcgagatca
     1081 atagcacgag gtaaaagatg cggatcgcac cttccaggag ctcgccgagt cgctcctgcg
     1141 taaccaactc gacgcgcttt acgcgaaccg tcttgatgag cgtgcccttc cactcacgca
     1201 ctctcgcgat gaacgtgtgg tagagcatga tgatcgcacg gatcacgacg atcataacca
     1261 cggtcagcag cgtcgcgccg agtaggcttg tcaagaggag ggtgtagttc cagccttcaa
     1321 taaacggaac cagatcgagg atatgccccg cgatccagat gaagaacata aacccgatcg
     1381 caaggcggag accttcgagc gcaatcatga gtaaatcggc gatttgcccg gcgctcagaa
     1441 cctcccaccc gccgatccgc aagccgcgta tccacgactg ctcccactga tccacgcgcg
     1501 gtagagccac gggaacagcc ggttgaggag gacataaatc gtcaccacga acaccgcaac
     1561 aaacagaccg ccgacggcat tcgcgatgat cgactcctcg tcgatggcga acacaccaag
     1621 taaatacgag aggatcgagt aggtcacgat gaggacgaac aggatgcgca gcgtgacgag
     1681 tatgttgacg accatgtgcg tcgcttcggc gaccgtcgcg agaacgaatc gaccgagccg
     1741 gatcgcgcgg atgtgtgtgc cttcccacga tcgggcgcgc tcatacaggg tcggccacga
     1801 acgatttgcg gaccgtgcga gaaataacgc gaacgctgca atgaggagtc cgcgcacgac
     1861 ctcgccgaat ggcgccgcgt tttccggtcg tgccacgccg actacggtaa tcatcgcaag
     1921 gtagcacagc gtgaggatga tgaaaaaacg gagtacccgc agtgtcacat ccgcaattgt
     1981 cgtccacgtc cgcgcgcgaa caagaatccg gttccacgca atgagcggct tgatccgacc
     2041 cgtccgtgcg agttcgtcaa gctttacccg cacacgcggt cgcagtatgc tgattccgcg
     2101 ccagatgacc accgttgcga taaggaggag aaacaccagc gagaggtcga gcggactgaa
     2161 cgcatcccac caccgagctg cttccgacag aatcgtgaag tcggctttta cgacgacggt
     2221 atccgatgtg acgaccaccg aggtgtcggt gatgacatgc gtgccaaccg ccagtgaatc
     2281 ggcgtccgtt ggagactggt tcgagtgcag ggtgtcacgt tgagcgtacg cattcccgaa
     2341 caccagtaaa gcggcgaaaa cgagagcggt aacaacagcg gttcgcgaca tccgattcat
     2401 ccttcccgac gcggacggaa tccgttcgca catcaacgca caaacagtcg aatgttcaat
     2461 atacagaggg aattgcggat ggtggaagat aaacgaaaga cacgtgtgga attccgcgtc
     2521 gcgcaccaat gtgaaatcgc cgaagagacg ggagacgcgc ctcactccgc cacatgcgta
     2581 attggatgca ggggaggatc actctgtggg tcaatcactc ccatttctcg cgacgcatgt
     2641 cgatatccca cactttcgtc gcaccaaccg atccggagat gagctcgagc tttccgtcac
     2701 catctatgtc tacaagctca agtatctcgc cgaactcgtc caaggattcc cacttcagcg
     2761 agcgtgtgaa cgcatcgagc acgaatccgg tgttcaggat gatttccggt tcatcgtctc
     2821 cgtcaacatc gccgacgacg atatccgtcg catcgaagat ctgctgtgaa cgccactctt
     2881 cgacgaagtg tttcccgtcg taaatgaata gtcgtccttc gctcatgaag agcaactcga
     2941 gctgcgggtc gtggtccact tgcccggttg tcagcgcttc gatggacagg aaatgcgtct
     3001 cggggctctc ccagcgcacg ctgaagtcgt caatgttcca tatcgcgagt tgcccacggg
     3061 tgttgtacgc aatgagcgca taccgaccgt cgacatcgag gtcggcgaac tgcacctctt
     3121 tgatacgggt cgccatcgac gggctcgacc agatgcttcg gtaatagcca ttttcgaatt
     3181 cgagcacgta cacgtttccg cgctcggtgc cgtacgcaat gcgtccacgc ggatcgtttg
     3241 gcaccgcggc gaaggtattg aaccgaatcg cgacgcggtg gaaacggtcc accatatttc
     3301 ggtttcgact cggtcgggtt tgcgcatcaa ctgtcgaaca gccgatcgag agaccaacca
     3361 ccaagatcgc accggcgatg attccccgag atatggattt cataagtgac ctccgatgat
     3421 gacgacgcgg gttattcggc gacaatacac
//
LOCUS       DSCC01000115            2002 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038437, whole genome shotgun sequence.
ACCESSION   DSCC01000115 DSCC01000000
VERSION     DSCC01000115.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038437"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(26..649)
                     /locus_tag="ENN56_01325"
     CDS             complement(26..649)
                     /locus_tag="ENN56_01325"
                     /inference="COORDINATES: protein motif:HMM:PF02631.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HDS74158.1"
                     /translation="MIRVNAAAVSSCSTVRRRLELDHALAIAAGIRVGATISRSDLAE
                     LVRKDSIASAEKDALALLTRRDHSEAELRKKLRAKDHDAAAIDVVVARCTDWGYLDDE
                     RIARQLVNDAINLKRLGPARIKQKLGELGIDKDVAREAISRAEDNAPSPVEQALGALA
                     SKRRSYARLDAETARRRMYGFLQRRGFDFDTVRAAVERFENELRDAE"
     gene            complement(728..1831)
                     /gene="recA"
                     /locus_tag="ENN56_01330"
     CDS             complement(728..1831)
                     /gene="recA"
                     /locus_tag="ENN56_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDS74159.1"
                     /translation="MAAKSSSKTEATTKETREKAVDLALGQIRKQFGDGSIMKMGDES
                     QKKIAMIPTGSIALDQALGIGGVPRGRIVELYGPESSGKSTLAMHIVAEAQKRGGIAA
                     YIDAEHAFDPTYGKLLGVDIDNLLISQPDSGEDALGITDTLVRSAAIDVIVIDSVAAL
                     VPRAELDGEMGDAHVGLQARLMSQALRKLTGSISKSHTCVIFINQIRMKIGVMFGNPE
                     TTTGGNALKFYSSVRMDIRRISTIKDGDETIGSRTRVRVVKNKVAPPFRMAEFDLMHG
                     EGISRAGELVDLGTELGIVSKSGTWYSIGEERIGQGRENAKQYLRDHPELMEELTDAV
                     REKLEIPIVKADGTIVIAGETVTPESPAESEGE"
BASE COUNT          328 a          643 c          586 g          445 t
ORIGIN      
        1 gaactgggaa ggcgtctgac cggagttact cggcgtcgcg gagttcgttc tcgaatcgtt
       61 ccacagccgc gcgcacggtg tcgaagtcga agccgcgccg ttgtagaaag ccgtacatgc
      121 ggcggcgcgc ggtttccgca tcgagccgcg cgtagcttcg tcgcttggac gccagcgcac
      181 cgagcgcctg ctcgaccggt gacggtgcgt tgtcctcggc acgcgaaatc gcttcccgcg
      241 ccacgtcttt gtcgataccg agttcaccga gtttctgctt gatgcgtgcc ggtccaagtc
      301 gcttcaagtt gatcgcgtcg tttaccaact gccgcgcaat ccgttcgtcg tcgaggtatc
      361 cccagtccgt gcaccgcgca acgacaacgt caatcgccgc cgcgtcgtga tctttcgcgc
      421 gcagtttctt ccgcagttcc gcctcggagt gatcccgccg cgtcaacagc gcgagcgcgt
      481 ccttctccgc cgatgcaatg gaatccttcc gcacaagctc tgcgagatcg gaccgcgaga
      541 tcgtcgcgcc gacacggatt cccgcggcga tagcgagcgc gtggtccagt tccaaccgac
      601 gccgcaccgt cgaacacgac gacacggcgg cggcgtttac gcggatcatt ggggatttct
      661 tcgatggctt tcaccgtcgg catacgagtg agctcctacg cacggtgata atgcgcggag
      721 agtccggtta ttcgccctcg ctttcggcgg gcgattccgg cgttacggtc tcaccggcga
      781 tcacgatggt gccgtcggct ttcacaatgg gaatctcgag cttttcccgg acggcatcgg
      841 tgagttcttc catcagctcc ggatggtcgc gcaggtactg cttcgcgttc tcgcgaccct
      901 gcccgatgcg ttcctcgccg atggagtacc acgtcccgga cttgctgaca atgccaagct
      961 ccgttccgag atcgacgagt tcgcccgccc gactgatacc ctcgccgtgc atgaggtcga
     1021 attccgccat gcggaagggt ggcgcgactt tgtttttcac gacgcggacc cgggtgcgcg
     1081 agccgatcgt ttcgtcgccg tctttgatcg ttgagatgcg tcggatatcc atgcgcacgg
     1141 agctgtagaa cttcaacgcg ttcccgccgg tggtcgtctc cgggttgccg aacatgacac
     1201 cgatcttcat acgaatctgg ttgatgaaga tgacgcaggt gtggctcttc gagatcgaac
     1261 cggtgagctt gcggagtgcc tgcgacatga gccgcgcctg caagccgacg tgcgcatcac
     1321 ccatctcgcc gtcgagttcg gcacgcggca ccaatgcggc gacggaatcg attacgatca
     1381 cgtcgattgc cgcgctgcga acgagcgtgt cggtaatgcc gagtgcgtct tcgccgctat
     1441 ccggttgact gatgagcaaa ttgtcgatgt ccacgccgag gagcttcccg tacgttggat
     1501 cgaaggcgtg ttccgcgtcg atatacgccg cgatgccacc gcgcttctgc gcttccgcaa
     1561 cgatgtgcat cgcgagcgtg ctcttcccgg atgactccgg gccatacagc tcgacgatac
     1621 gaccgcgcgg cactccgccg atcccgagcg cctgatcgag cgcaatcgag ccggtcggaa
     1681 tcatcgcgat cttcttctgc gactcgtcgc ccatcttcat gatcgagccg tctccgaact
     1741 gtttccggat ctggccaagc gcgagatcaa ccgccttttc gcgagtttcc ttcgtggtcg
     1801 cttcggtctt ggaactgctt tttgccgcca ttcgcgtata ctccccgtga gaacttcgat
     1861 gtgatgcgcc gaatgtacca cgagcctcgg aacgatcgaa cccgtcgtcg gtgcttttcg
     1921 tgtcgatttc ctcgtctgac tccgcgttat cgtcgacgac gtccgttgtg ccaccctctg
     1981 gattgctgac ctcgtcgaaa tc
//
LOCUS       DSCC01000116            3199 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020505, whole genome shotgun sequence.
ACCESSION   DSCC01000116 DSCC01000000
VERSION     DSCC01000116.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020505"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..451
                     /locus_tag="ENN56_01335"
     CDS             <1..451
                     /locus_tag="ENN56_01335"
                     /inference="COORDINATES: protein motif:HMM:PF00198.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HDS74160.1"
                     /translation="GYSADGIERCRDVNIGVALGLDAGVVVPVIHEVNRKSVDEIARE
                     LGEKTDRAKRGVLLAEDVQGSTFTLSNLGMFGVDEFQAILNAPDAAILAVGAVSKQAV
                     VRGDVLAVGTVMTGTLTVDHRVADGVAAAKWMAAFADVLTNPIELVM"
     gene            549..1277
                     /locus_tag="ENN56_01340"
     CDS             549..1277
                     /locus_tag="ENN56_01340"
                     /inference="COORDINATES: protein motif:HMM:PF01243.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDS74161.1"
                     /translation="MERREIFVAIDQGRLDELVTSTDSTKELNEIHNALFHRALEVGV
                     PKQELIRRIDQATYAAARESNMDERDIKQMSRKTLEDYDPKLAIQVYRTQINAMYTAY
                     MTAIENRETGEERPEEELFNTGAYGQTLSTASLDGTPVVAVFTQLAVIEDEKIRFGRI
                     GQEQAYKNLKENPKCVFTAIGPSNDPSKVDLVSITAELVDDIDSGEGLERGRAEFGHA
                     VSNLLIFSVTDVKMNHLPKPIHMT"
     gene            1287..1898
                     /gene="rdgB"
                     /locus_tag="ENN56_01345"
     CDS             1287..1898
                     /gene="rdgB"
                     /locus_tag="ENN56_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDS74162.1"
                     /translation="MAQDRTRIVIATRNVGKIREIVELYSDLPIEWVPIDSLGVSPEI
                     EETGETFRDNAILKACGISRWSGFPTLADDSGLIVDALDGAPGVRSARFAGEHASDAD
                     NVRLLLERLSNVPDERRTARFHASLIVWWSESEYDEADGVCEGRIARAPSGNTGFGYD
                     PVFVPDGYERSFAVLGPDVKNRLSHRARALKRLRPMLERRLGV"
     gene            1921..2712
                     /locus_tag="ENN56_01350"
     CDS             1921..2712
                     /locus_tag="ENN56_01350"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS74163.1"
                     /translation="MDLCVLASSSSGNSVAVRSGESILLIDAGLSARAIERKLDEIGW
                     SSREICGIVLSHEHQDHCRGASLLSRRYGVPVYGTVGTLEALSSQWRGDETLNEIKNG
                     YSFSVGPFVCEPFSIPHDVADPAMFVVRCDGSSIGFATDLGRVTSLVRNKLASTDLAL
                     IEANHDPDRLRWGDYPWAVKQRISSPHGHLDNEQAATLAVELASAGVEHIVLGHLSPR
                     HNDVEQVAQTIGRALVETRLQPRVTVIAPNERSGIISVVNGGTRT"
     gene            2709..>3199
                     /locus_tag="ENN56_01355"
     CDS             2709..>3199
                     /locus_tag="ENN56_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDS74164.1"
                     /translation="MSWTILVTGGAGFIGSNFIRYTLSTYSDAQIINLDKLTYSGNLA
                     NLRDVEHDTRYEFVRGDIADEQIVNPLVERADAIVNFAAESHVDRSIQDPHVFLRTNI
                     DGPYALLEASRKTGIERFLQVSTDEVYGEVMTGHSREDDTLLPRSPYSASKAAGELLA
                     RSY"
BASE COUNT          752 a          812 c          946 g          689 t
ORIGIN      
        1 ggggtacagt gccgatggaa tagagcggtg tcgcgacgta aatatcggtg tagcgctcgg
       61 actcgacgcc ggagtcgtgg taccggtcat ccacgaagtc aatcggaagt cagttgacga
      121 gattgcgaga gaactcggag aaaagactga ccgagcgaaa cgtggagtcc tattggctga
      181 ggacgttcag ggaagtacgt ttaccttgtc caatttgggg atgttcgggg tcgacgagtt
      241 tcaagcgatt ctcaacgcac ccgacgcggc gatactcgcc gtcggagccg tttccaaaca
      301 ggcggtcgtt cgaggggacg tactcgccgt cgggacggtg atgactggaa cgcttacggt
      361 agatcaccgt gttgcggatg gagtcgcagc tgcaaagtgg atggcagcgt ttgccgacgt
      421 tctgacaaat ccgattgagc tcgtcatgta accgatttcg ttacccggta ctcactctgt
      481 tttacgcaac ggttcattgc ggtaacgcgt agaagcgctc cccgtggggg gacgaggtgg
      541 gaggacacat ggaaaggcgt gagattttcg tagcgatcga tcaagggcgc ttggacgaac
      601 tcgttacgag tacggattcg acaaaggaat tgaacgagat tcataatgcg ctgtttcatc
      661 gcgcgcttga agtcggcgtg ccgaagcagg aattgatccg tcgaatcgac caagcaacat
      721 acgctgctgc gcgtgaatcg aacatggacg agcgcgatat caagcaaatg agccgaaaga
      781 cgctcgagga ttacgatcca aagctcgcga ttcaggtgta tcggacgcag atcaacgcaa
      841 tgtacaccgc gtatatgact gccatcgaaa accgcgaaac gggcgaagaa cgtcccgaag
      901 aggaactctt caacacgggc gcgtacggtc agacactcag caccgcatcg ctcgacggga
      961 cacccgtggt agctgtcttt acacaactcg cagtaatcga agacgagaaa atccggttcg
     1021 ggcgcatcgg acaggagcaa gcatacaaga atctcaaaga gaatccgaag tgcgtcttca
     1081 cggcgatcgg tccgagtaac gatccgtcga aggtcgattt ggtttcgatt actgccgagc
     1141 tcgttgacga tatcgattcc ggcgaaggac ttgagcgcgg gcgtgccgag ttcggtcacg
     1201 ccgtttcgaa cttgttgatc tttagcgtta ccgatgtgaa gatgaaccat cttccaaaac
     1261 cgatacatat gacgtaagtc gcgtcgatgg ctcaagaccg aacaagaatc gtcatcgcaa
     1321 cacggaacgt ggggaaaata cgcgagatcg tcgaactcta ttccgatctg ccgatcgaat
     1381 gggttccgat agactctttg ggcgtttcgc ctgagatcga agagaccggt gagacgtttc
     1441 gcgataacgc gattctgaag gcgtgcggaa tatctcgttg gtcgggattc cccacgcttg
     1501 cagacgatag cggactcatt gtcgacgcac tcgacggtgc tcccggagta cgttctgcac
     1561 gttttgcggg cgaacatgcg agcgacgccg ataacgtgcg actgttgctc gaacgtcttt
     1621 cgaatgtccc agatgagcgt cgcacagcgc gattccatgc gtcacttatc gtttggtgga
     1681 gcgaatccga atacgatgaa gcggatggcg tatgtgaagg tcggattgca cgtgcgccgt
     1741 ccggaaacac tggtttcgga tacgatccgg tgtttgtgcc cgacgggtat gaacgatcgt
     1801 tcgcggttct tggacccgat gtgaaaaacc gactaagtca ccgcgcaaga gcactcaaac
     1861 gactccgtcc tatgctggag cgccgactgg gtgtttgaca gtcctgaaaa agcgcagaca
     1921 atggacttat gtgtccttgc gagcagtagc tcgggtaatt ctgtcgcggt tcgatccggc
     1981 gaaagcattc tcctgattga tgctggtctc agtgcacgag ccattgaacg taagctcgat
     2041 gagatcggat ggtcgagtag agagatttgc ggtatcgttc tcagtcacga acatcaggat
     2101 cactgccgtg gagcgagtct gctcagtcga aggtacggag tccccgttta cgggactgtc
     2161 ggaacgctcg aggctctatc gtcgcagtgg cgaggagacg agacactcaa cgaaatcaag
     2221 aacgggtatt cgttttctgt cggtccgttt gtgtgtgaac cgttttcgat accgcatgat
     2281 gtcgctgatc cggcgatgtt cgtcgtccgg tgtgatggga gttctattgg attcgcgaca
     2341 gacctcggac gtgtgacgag tctggtgcga aacaagctcg cgtcgaccga ccttgctctc
     2401 atcgaagcaa accatgaccc ggaccggctc cggtggggcg attatccgtg ggcggttaag
     2461 caaaggatat caagtccgca cgggcatctc gacaacgaac aggcggcgac tttagcggtt
     2521 gagctcgcaa gcgcaggtgt cgaacacatc gtactcggtc acctcagccc gcgacacaac
     2581 gatgttgaac aagtcgcgca aacgatcggt cgtgcgctcg tcgagacacg actacagcct
     2641 cgcgtcaccg ttatcgctcc gaacgagcgg tcgggcatta tctccgtagt aaacggagga
     2701 actcgtacat gagttggaca attctcgtta ccggcggtgc cggattcatt ggatcgaatt
     2761 tcatccgata cacactctca acgtattccg atgcacaaat tatcaatctc gataaactga
     2821 cgtactccgg gaaccttgcg aatctccgcg acgtggaaca cgatacgcgg tacgagttcg
     2881 tccgcggaga catcgctgac gagcagatcg tcaatccgct tgtggaacga gccgacgcga
     2941 tcgtcaactt tgcagcggag agtcacgttg atcgttcaat tcaagatcca cacgtgtttc
     3001 tccgaacgaa catcgacgga ccgtacgcgt tgctcgaggc ttcgcgaaag acgggtatcg
     3061 agcgctttct gcaagtgagt actgacgaag tctacggcga agtcatgacc gggcactcgc
     3121 gcgaggatga cacgctgctt ccgcgaagcc cgtattcggc gagtaaagct gcgggtgagt
     3181 tgctcgcgcg ctcgtatta
//
LOCUS       DSCC01000117            3636 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017203, whole genome shotgun sequence.
ACCESSION   DSCC01000117 DSCC01000000
VERSION     DSCC01000117.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017203"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="ENN56_01360"
     CDS             complement(<1..1133)
                     /locus_tag="ENN56_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_661670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDS74165.1"
                     /translation="MLTWLTFVPLIGAGVILLIPTGGGRGDDSSDGEPIGWKAIRIVA
                     FATTAVTLVLAILLFVAFDRTTSAFQFEQIVTWIPTFNIHYHVGVDGVSILMVLLTQL
                     IGIIGVLASWRISKAVKGYFSLYLLLQTGMTGVFVALDFVLFYIFWEVMLLPMYFLIG
                     IWGGPRKEYAAIKFFLYTLLGSVLMLLAILGLYFNMKEPTFDMIAMMDQSAYAPDGWL
                     MSGLSTWFFSFTPKQLVWLAFFVAFAIKIPAFPFHTWLPDAHVEAPTAISVILAGVLL
                     KMGTYGIFRVNFPMLPDTTVEFAKFIAVIGVINIIYGALCAMAQKDLKSLVAYSSISH
                     MGYVMLGMVSWHHAAGINGAMFQMFNHGTITAMMFLLVGVIYDR"
     gene            complement(1189..3291)
                     /locus_tag="ENN56_01365"
     CDS             complement(1189..3291)
                     /locus_tag="ENN56_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDS74166.1"
                     /translation="MATYALILLLPLVAFAIQMLIGKRLPRGGDWVVLAAIAGSFALS
                     AVVFAQALFGHAFADGAYQWVMTDWLRSSELAPSGWTLPMGILVDNLTAMMLIVVSGC
                     SALIFLFSVGYMHDHPRYSRFFGYLSLFAFSMLLLVLGNNLIALYVGWELVGLSSYLL
                     IGFLHDEEIVSPDINPGYAAKKAFLTNRVGDLGFFLGILTLFFMTGAVQYMDVFDAVE
                     NGAFDVRPWLLTVAGILLFTGAIGKSAQFPLHIWLPDAMAGPTPVSALIHAATMVAAG
                     VYMVGRIYPLLTPDALLVVAYVGAITAFFAATIAIVMTDIKRVLAYSTISQLGYMIFG
                     LGVGAYSAGLFHLMTHAVFKALLFLASGSVIHAMHSAYHHAHAEWNPETGENDAQDMR
                     NMGGLRKYLPITFGTMFIATLAISGVPFTSGFLSKDAILGGALAFGWYLHPQHFLIPL
                     FGFLAAGMTAFYMFRLIFMTFFGENRTGEHARTGLRESPKTMTIPLIVFAVFSLWIWY
                     NWDPTVLVANGWVDHFINKPDTVSMAGGFGAAMHGAYEQAAHHAHVPAMLLSLLVAGC
                     GIALAWVLYKRGAISLEAWREKDTALYRLLANKYYVDEFYEKTVIALTYAISKVSALF
                     DTYVVDGIVNGAAAVARWLSALSGMIDHRGVDGLVNAIADVTQTLGDRVRRVQTGRVQ
                     NYLVFAVLGAIVLIIIVR"
     gene            complement(3306..3611)
                     /gene="nuoK"
                     /locus_tag="ENN56_01370"
     CDS             complement(3306..3611)
                     /gene="nuoK"
                     /locus_tag="ENN56_01370"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDS74167.1"
                     /translation="MIPLNAYLLVSAMLFSLGVVSILTRRNAVAVLMGVELMLNAANL
                     NFVAFARFSATLINGQMFAIFSIVMAAAEVAVALALVLNIYRHIGTIDVDEADTLRG"
BASE COUNT          952 a         1065 c          955 g          664 t
ORIGIN      
        1 cggtcataga tcacgcccac gaggaggaac atcatcgcgg taatcgtgcc gtggttgaac
       61 atctggaaca tcgcaccgtt gatacccgca gcatggtgcc acgacaccat accgagcatg
      121 acgtagccca tgtggctaat cgacgaatac gcaacgagcg atttcaggtc cttctgtgcc
      181 atcgcacaca gcgcgccgta gatgatgttg atcacaccaa taaccgcgat aaacttcgcg
      241 aattccacgg tcgtatccgg caacatcgga aaattcaccc tgaagatgcc gtatgttccc
      301 atcttcagga gcacgcccgc aaggatcacg ctgatcgcgg tcggcgcttc gacgtgtgcg
      361 tcgggtaacc atgtatggaa cgggaaggca gggattttga tcgcgaatgc aacgaagaac
      421 gcgagccaaa caagctgctt cggcgtaaac gagaagaacc atgtcgagag accgctcatg
      481 agccagccgt cgggagcata cgcagactga tccatcatcg cgatcatgtc gaacgtcggt
      541 tccttcatgt tgaagtagag accaagaatc gcgagcagca ttaggacgct gccaagcaat
      601 gtatacagga agaacttgat cgccgcgtat tctttccgtg gaccgcccca aatgccgatg
      661 aggaaataca tcggtagtag cattacttcc cagaaaatgt agaacaacac gaaatcgagt
      721 gcgacgaata cacccgtcat accggtttgc agcaacagat acagcgagaa ataccctttt
      781 accgctttcg atatccgcca cgacgcaagc acgccgatga taccgataag ctgcgtcaaa
      841 agcaccatga ggatactgac gccatcgacg ccgacgtggt agtggatatt gaatgtcgga
      901 atccacgtca cgatctgctc gaactggaac gcgctcgtcg tccgatcgaa cgcaacaaag
      961 agcagaatgg cgagaacgag cgttaccgct gtcgtcgcaa atgcaacgat gcgaatcgct
     1021 ttccaaccga tcggttcgcc atctgacgag tcgtcgcctc gtccgccacc ggtcggaatc
     1081 agcaggatca cccctgctcc gatgagcgga acaaacgtca gccaagtgag gatggtatcc
     1141 atcacaataa tgcccttctc gatctgcgcg ggttatgagc tggtctcgtt atctgacgat
     1201 gatgatcaaa acaatcgcac caagcactgc aaatacgagg taattctgca cacggcccgt
     1261 ctgcacccga cgaacacgat caccgagggt ctgcgtaacg tctgcaatag cgtttaccag
     1321 tccgtccact ccacgatgat cgatcatacc actcagcgct gacaaccaac gtgcaaccgc
     1381 cgcggcgccg ttgacgatgc cgtcgacgac ataggtatcg aatagtgcag aaaccttgct
     1441 tatcgcgtac gtcaacgcga taacggtctt ctcgtagaac tcgtctacgt aatacttatt
     1501 ggcgagcaat cgatagagcg ccgtatcctt ctcgcgccat gcttcaagtg atatcgcccc
     1561 gcgcttgtat agcacccacg ccaatgcaat cccgcatcca gctaccaaga gcgaaaggag
     1621 catcgccggc acgtgcgcgt ggtgagccgc ttgttcatac gccccgtgca tcgccgcgcc
     1681 gaacccgcca gccatggaaa ccgtgtccgg cttgttgata aagtgatcga cccagccatt
     1741 cgcgaccagt actgtcggat cccagttgta ccatatccac agcgagaaca ccgcgaagac
     1801 gatcagcgga atcgtcatgg tcttcggcga ttcacgaagt ccagtccgcg cgtgctcacc
     1861 agttcggttc tcgccgaaaa acgtcatgaa gatcaggcga aacatgtaga acgctgtcat
     1921 gcccgccgcg aggaacccga acagtggaat caggaagtgc tgcggatgta gataccatcc
     1981 aaacgcaagc gcaccaccga gaatggcgtc tttactgaga aacccgctgg taaacggcac
     2041 accgctgatg gcgagcgtcg cgatgaacat cgttccgaat gtgatcggca ggtacttccg
     2101 caagccaccc atgttgcgca tgtcctgcgc gtcattctcg cccgtttccg gattccactc
     2161 ggcgtgcgca tgatgatacg cgctgtgcat cgcgtgaatc acactgcccg acgcgaggaa
     2221 gagaagcgcc ttgaacactg catgcgtcat gagatggaac aagcccgccg aatatgcacc
     2281 aactcccagt ccgaagatca tgtaccccag ctgactgatc gtcgaatacg ccagcacgcg
     2341 cttgatatcg gtcatgacaa tcgcgatggt tgccgcgaaa aacgccgtaa tcgcaccgac
     2401 atacgccacg acgaggagcg catccggcgt gagcaacgga tagatacgtc cgaccatgta
     2461 gacgcccgcg gcaaccatcg tcgccgcgtg aatcaatgcg ctcaccggtg tcggacccgc
     2521 catcgcatcg ggaagccaga tgtgcaaagg gaactgcgct gacttgccga tggcacccgt
     2581 aaacaggaga atgccggcca ctgtcagtaa ccatggacgc acgtcgaacg cgccgttctc
     2641 aactgcgtcg aagacatcca tatactgaac cgcaccggtc atgaagaaaa gcgtcaggat
     2701 cccgaggaag aaaccgagat cgccgacacg gttcgtaaga aacgctttct ttgccgcata
     2761 tccggggttg atatccggcg agacgatttc ttcgtcgtga agaaacccga tcaggaggta
     2821 ggaactcagg ccaacaagct cccatccaac gtacagcgca atgagattgt taccgagtac
     2881 gaggagcagc atgctgaacg cgaaaagcga tagatatccg aaaaaccggc tgtaccgcgg
     2941 atggtcgtgc atgtatccga cgctgaacag gaagatcagt gcggagcatc ccgaaacaac
     3001 gatcaacatc atcgccgtga gattgtcgac gaggataccc atcggaagcg tccaaccgct
     3061 gggcgcaagt tccgacgagc gtaaccagtc ggtcataacc cactgatacg caccatcggc
     3121 gaacgcatgc ccgaataacg cctgtgcgaa gactaccgcg gaaagcgcaa acgacccggc
     3181 gatcgcggcg agtacaaccc agtcgccccc gcgcggaagc cgtttgccga taagcatctg
     3241 gatcgcaaat gccacaagtg gaaggagcag gatgagcgca tatgtcgcga tggatgttgc
     3301 cacggtcagc ctctcaaagt atccgcttca tccacatcga tggtgccgat gtgccgatag
     3361 atgttcaaca caagcgcgag cgcgacggcg acttccgcgg cagccatgac gatgctgaaa
     3421 atcgcgaaca tctggccgtt gatcagcgtc gcagagaacc gcgcaaacgc gacaaagttc
     3481 aggtttgcgg cattcaacat gagttcaaca cccatgagca cggcaacagc attccgtcgc
     3541 gtgagaatac ttacgacacc aagagaaaag agcatcgcgc tgacgagcag gtacgcattc
     3601 agcggaatca tgtcacgcgt cctcccgttt cgctac
//
LOCUS       DSCC01000118            2638 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026468, whole genome shotgun sequence.
ACCESSION   DSCC01000118 DSCC01000000
VERSION     DSCC01000118.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026468"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..464)
                     /locus_tag="ENN56_01375"
     CDS             complement(<1..464)
                     /locus_tag="ENN56_01375"
                     /inference="COORDINATES: protein motif:HMM:PF00583.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS74168.1"
                     /translation="MPDSPLPNVHKSIYIRYRLRSSTYGYVTLRESMGSIRGPRALCL
                     DEFKSLHRLQDTVFRPGPNTSMFDEFPTLFSPENRERLRVISVDGEIVSAICYQIRPA
                     IIYGVTLAVASLGAVCTYESYRGHGFATQLLDDTFAAARKEGADVMLISGQRG"
     gene            562..1446
                     /locus_tag="ENN56_01380"
     CDS             562..1446
                     /locus_tag="ENN56_01380"
                     /inference="COORDINATES: protein motif:HMM:PF00701.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDS74169.1"
                     /translation="MLESLSHLRFTTPLVTPFGRREGVQSDRIAELVRRQKTAGADAV
                     LVASPLGEGDTLSSDERGLLAQTAVKTARDVDIAVLVDISAPSVRAAVIAAEHAMSIG
                     ADALVMALPSLRSYDTVTCGLVVNEIARATNLPLVIRAPDTKTFCLSLEVINQLARVP
                     IVQGCVTDDLEEALRLRNETPLTVLMASDFLIGPSLLAGISGIVSPVANLVPESIARI
                     VRASMIGNIDEVRSAQRWITGLCDLIGTELVVPRMKAALQLLGIPVGIGRLPIRELTD
                     LEIEALRAALRQWGIGVS"
     gene            1536..>2638
                     /locus_tag="ENN56_01385"
     CDS             1536..>2638
                     /locus_tag="ENN56_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74170.1"
                     /translation="MGELKTPFQLRGWGIRTWNSVDRFETAVERAITRAASHGITTID
                     LHDGTVPPGIGWVDLFAEYKQVQALRDADILTYHGEQISREQREGYRNRFRELCRKIK
                     AHGIDIHVWYHVLRDLPEEWLDVEPTVSRLEGRRLWQVLGGIVDDFLMAVPEVDALTV
                     TGQQALTAGMRDTSSVTSGERLRAIYQCLYEACRRQRRRLVIREIGTTAAEKEAFVHA
                     IGPLPPDIAIMVKDVQGDWYHLDAPLNPLFLRLRNKQLIVETDLYGVHWGHLDVPLCR
                     LRQIHTAVRSWLPLSISGAIARIKVVEQPDSPSVHVFDTPNEANISALSHLLQNPYPD
                     PDLDSKDVDAFDFRLWIDWLRNRYGEDASPYVIA"
BASE COUNT          549 a          681 c          786 g          622 t
ORIGIN      
        1 ccccgttggc cggaaatgag catgacatcg gcaccttcct tgcgggctgc cgcgaaggtg
       61 tcgtcgagca attgcgttgc gaaaccatga ccccgatagc tttcgtacgt gcagacggct
      121 ccgagcgacg caacggcgag tgttaccccg taaataatcg ctggtcggat ttgatagcag
      181 atcgcggaga cgatttcgcc atcgacggaa ataacgcgga gacgttctct gttttccgga
      241 gagaacagcg tcgggaattc gtcgaacatc gatgtattcg ggccgggacg aaacaccgtg
      301 tcttgaagcc ggtgtaagct cttgaattca tcgaggcata gcgcacgcgg tccgcgtatc
      361 gatcccattg attccctcag cgtcacatat ccgtacgtcg aacttcggag tcgatatcga
      421 atgtagattg atttatgaac gttgggcaag ggcgaatccg gcatctcccc ctgccgattg
      481 atcctcgtgt tgactacctt ttttcgttgg ttcgtcagtc cgatgtgctc ggtacatcac
      541 gcataggtcg cggagaacga cgtgctcgaa agcctttctc atcttcggtt tacgacgccg
      601 cttgttacgc cgtttggtcg gcgcgaaggt gtgcagtcgg accgtatcgc cgaactcgta
      661 cgacgacaaa aaaccgccgg agccgatgcg gttctcgtgg cgagcccgct cggagaaggc
      721 gatacgttaa gctccgatga acgcggtttg cttgcgcaga cggcggtaaa gacggcgcgc
      781 gatgtcgata tcgcagtgct tgtggatatt tcggcgccga gtgtgcgagc agccgtaatc
      841 gcagcggaac acgcgatgag tatcggtgcg gacgctttgg tcatggcgtt gccatcattg
      901 cgttcgtatg atacggtcac gtgtgggctg gttgtaaacg agattgcgcg ggcgacgaat
      961 ctaccgttgg tgatccgagc acccgatacc aagacgtttt gtctgtcgct tgaggttatc
     1021 aatcaactcg cgcgcgtgcc gatcgttcag ggatgcgtta cggacgacct cgaggaagca
     1081 cttcgccttc ggaatgaaac gccgctgact gtgttaatgg cgtcagattt ccttatcggt
     1141 ccatcgctgc tggcgggaat cagtgggatt gtctctccgg tggcgaatct ggtcccggaa
     1201 tccattgcgc gtatcgttcg cgcttccatg atcggcaata tcgacgaggt gcggagtgct
     1261 cagcggtgga taacgggatt gtgtgatctc atcggtacag agttggtggt tccccgaatg
     1321 aaagcggcgc tccagttact cggtattccg gtggggatag gacgtctccc catacgtgaa
     1381 ctgacggatt tggagatcga agcgcttcgc gccgcgctac gacagtgggg aattggagta
     1441 tcgtgatcgt atcgtaaata gagtgcattc ttttttgtat ctgacatgtc tgcagtcgcg
     1501 tttggtatgt gtgagaagtg gaaggctgaa agcacatggg tgagctcaaa accccgtttc
     1561 aacttcgcgg atggggtatt cgcacgtgga attccgtaga tcgttttgaa acggcggtgg
     1621 agcgcgcgat taccagagcg gcgtcacacg gcataacaac gatcgacctg cacgacggta
     1681 cggtacctcc gggcattgga tgggtcgatc tattcgccga atacaagcag gttcaggcgc
     1741 tccgtgacgc cgatattctc acgtaccacg gggaacagat cagccgcgaa cagcgcgaag
     1801 gatatcgaaa ccgatttcgc gagctttgtc ggaagatcaa ggcgcatggt attgatatcc
     1861 acgtctggta tcacgtctta cgcgatcttc ccgaagagtg gcttgatgtc gagccgacgg
     1921 tgtcacgact cgaaggacga cggctctggc aggtgcttgg cggtatcgtc gacgattttc
     1981 tcatggcggt gccggaggtc gatgctctca ccgtcaccgg tcaacaggca ttgaccgcag
     2041 ggatgcggga tacgagtagt gtcacgtcgg gcgagcgtct tcgcgctatt taccagtgcc
     2101 tttacgaagc gtgtcgtagg caacgtcggc gcctcgtcat ccgtgagatt ggcacgacgg
     2161 cggcggaaaa ggaggcattc gttcacgcaa ttggtccgtt gccgccggat atcgcgatca
     2221 tggtgaaaga cgtacagggg gattggtacc acctcgacgc accactcaat ccactgtttc
     2281 tccgtttgcg taacaaacaa ctcattgtcg aaacagatct ttatggcgtg cactggggcc
     2341 acctcgacgt gccactctgc cgattacgtc agattcatac ggccgtacgg tcatggctcc
     2401 cgctgtcgat ctcgggggcg atcgcgcgga tcaaagtcgt cgaacagcca gactccccct
     2461 ctgttcacgt tttcgatacg ccgaatgaag ccaatatttc ggccttgagt catcttcttc
     2521 aaaacccgta tcccgatccg gacctcgatt cgaaagatgt cgacgcgttt gacttccgat
     2581 tgtggataga ttggttgcgg aatcggtacg gcgaagatgc gagtccctat gtaattgc
//
LOCUS       DSCC01000119            2925 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023149, whole genome shotgun sequence.
ACCESSION   DSCC01000119 DSCC01000000
VERSION     DSCC01000119.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023149"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(1086..>2925)
                     /locus_tag="ENN56_01390"
     CDS             complement(1086..>2925)
                     /locus_tag="ENN56_01390"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13205.4,HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74171.1"
                     /translation="IVVLLNTGTGEYTTPLDTVIAGGQLRDLFVGAISSEAGSVSIIA
                     APSVSNEPVLILERPTMRAEASPLQSENESPLSVDGSLPADNDTEVSTSAEFEVWFNA
                     PVNNQGRALLDSGWVTITGTIPGVGTRDIGVSAVRSVTDPRPSMSARYVLVPQEYFLP
                     LEEITVTLAGVRGVAGVGQNAVYFLLPEPKVIRFTTSTLKVIDSQPASDEEFVGLDDG
                     VVLRFNGPIDSTTANSSTIVIYGTGGRVIPYGSRYDESEQTLHLDPSVPFHPYERITV
                     ATTSGITDTLGRSIFEGHSFSFWATGPQVVATAPLDGAVGSPNLISVSFNTEMKSSSP
                     EAFRVYGSQSGRHVIAGVSYSPSNTRVLEIVPRGSFHSGETVTVVVTTDVESVESTKL
                     AKPHVWSFTAQPSAPAELRPVTPQFTPSHGGGTVAAGRFSVDGGGMVLADTSGVITAF
                     TRGADGWYPSESFSGEKTRRVLRGADLNGNGLLDLIEVLPDSDLVRVYLNLSDATGAI
                     AFGEPDEYHVGRFPAGVYIGDLNGDGWLDLATPNTAANDVSVLLNTGDGRFAEERYYR
                     VGNHPRAIVGADLDGDGDIDLVVANASDGTVTLLRNQTQIAPPSDR"
BASE COUNT          685 a          840 c          781 g          619 t
ORIGIN      
        1 catcgtacgt gcgtacgacc cgaccggaca tggagcgcgt ggcaaattgg cgctggcact
       61 accgatcgtg aaggcattct caagtacgct tatggagaat gaccgtgggg agattcaccc
      121 ggacgaatca tccatcgact ggtcgatgga accgttcgca atagagaccg tgcacctctt
      181 taccaacggc gacacatggt cgaatctcgg tcaagcgaca ctcggaccaa tcactgaatc
      241 ggtacaacca gtttggtgcc gatcatggaa acacaattcc ggtgcgcatc cacttgggta
      301 tttaccggtg gcaatacttc ttgacggcga tcttccgatt gaaaacgagg gcggaaactt
      361 cccaaccgtt gcgcgcgtcc gcgtgacaat cgtcaataat tacacggatc gttcgatcga
      421 cggatcggct cagattacgg cgccttcaaa ctggacgatt ctcccgaaaa ccgttcagta
      481 cgacctcgcg ccgcgcgagc acctcgttga ggatgtgaca attacctttg atcgtccacc
      541 ccgtgtcggc cttctcaagg cacgcctcgt ccatgagggt caggtttacc aggacgtaat
      601 agaggcaggt gttgataatc gagtcgagat cgggggaggc ggaggtgaac gactcaaccg
      661 tttagacgtt gtccaggaac ggcctatcga gtggtcagtg ctacgagatg gccaagatat
      721 cgtcgttcac gtacgaaatc cctggcgcga gcaacttgat gccgagctat cgattattac
      781 cccactggaa atgtggggga aagatgccgg cgcgttcgag cttgcggaga tttcgccgcg
      841 aacgatcggt ctgaacgtac ctccactaca acagcgtatt ctacgattct cgttacacca
      901 tacaggctcg catggtccgc tattctgggc ttgggcgaaa ctcgtgtatc acggtaagcc
      961 cgattaccgc ccggttcccg gaactacggc gtaatcacgt ttcaattcga ggcgaactca
     1021 caaacggggc gtcgacgaat agtcggcacc ccgtttccat cgagtatcgg agattctacg
     1081 actatttacc gatcggacgg cggagcgatc tgcgtctgat ttcgcagcag cgtcacggta
     1141 ccgtcgcttg cattcgcgac cacaaggtca atatcgccgt caccgtcgag gtctgctcct
     1201 acaatcgccc gtggatggtt gcccactcgg tagtaccgtt cttccgcaaa tcgtccgtcg
     1261 cctgtgttca gtaacaccga aacatcgtta gccgccgtat tcggcgtcgc cagatcgagc
     1321 catccgtcgc cattgagatc cccgatgtac acacccgccg gaaacctacc gacatggtat
     1381 tcatccggtt ccccgaacgc aattgcacca gtcgcatcac tcaggtttag atacacgcga
     1441 acgagatcgg aatccggaag cacctcaatg agatcaagca atccgttccc gttcagatcg
     1501 gcgccgcgaa gaacacgacg agtcttttct cctgaaaagc tttcgcttgg ataccaccca
     1561 tcggcccccc gtgtgaaagc ggtaatcacg ccgctcgtat cggcgagcac cattcctccg
     1621 ccatctacgc tgaatcgccc cgcggcaact gttccgccgc catgcgacgg agtgaactgc
     1681 ggcgttacgg gacggagttc tgcaggtgcg gaaggttgcg cggtaaacga ccagacgtga
     1741 ggctttgcga gtttcgtact ttcgacagat tcgacatcgg tggtaacaac aacggtgacc
     1801 gtttcgcctg aatggaacga accgcgcggc acgatctcga gtacacgagt attcgatggc
     1861 gaatacgaca cgccggcaat cacatggcgg ccactttgcg agccgtatac gcgaaatgct
     1921 tccggagagg acgacttcat ctccgtgtta aacgacactg agatcagatt cggcgaaccg
     1981 accgcaccgt ctaaaggtgc agtcgcgacg acctgaggtc ccgtcgccca aaacgagaac
     2041 gagtggccct cgaatatcga tctaccgaga gtgtccgtga tacccgatgt cgttgcaacg
     2101 gtaatccgtt catatgggtg aaacggaaca gatggatcga ggtgcaaggt ttgttctgac
     2161 tcatcatatc gcgatccgta cggtatcact cgaccgccag tgccgtaaat aacgatcgtc
     2221 gaggagttcg ccgtcgtaga atcaatcggt ccgttgaagc gaagaacaac accgtcatca
     2281 agcccgacaa actcttcgtc tgacgccggt tgagaatcaa ttaccttgag cgtagacgta
     2341 gtaaagcgga taactttcgg ctcaggtaaa agaaagtaca ccgcgttttg tccgacgcct
     2401 gcaacgccgc gtactcccgc cagcgtcacc gtaatctctt cgaggggtag gaaatactcc
     2461 tgcggtacga gtacgtaccg cgcgctcatg gacggacgcg gatctgtaac ggaccgaact
     2521 gcagatactc cgatatcgcg cgttcccacg ccgggaatgg taccggtaat cgttacccac
     2581 ccggaatcga gtagcgcacg tccctggttg ttcaccggcg cattgaacca aacttcgaac
     2641 tctgcagatg ttgacacttc ggtgtcgtta tcggcgggta atgacccgtc cacgctgaga
     2701 ggcgattcgt tttcagattg caacggactc gcctcagcac gcattgtcgg tcgctcaagt
     2761 atcaacaccg gctcattgga aaccgatggc gccgcgataa tggaaacgct cccggcttca
     2821 gaactgattg cgccaacaaa taaatctcga agttggccac ccgcaatcac cgtgtcgagc
     2881 ggggttgtat attcgcccgt gccggtgttc aggagcacta caata
//
LOCUS       DSCC01000120            2096 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036148, whole genome shotgun sequence.
ACCESSION   DSCC01000120 DSCC01000000
VERSION     DSCC01000120.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036148"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="ENN56_01395"
     CDS             complement(<1..1277)
                     /locus_tag="ENN56_01395"
                     /inference="COORDINATES: protein motif:HMM:PF13320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDS74172.1"
                     /translation="MPIRTPDPRSDMPPGMWADALVPRTNPYTGEPVAGPQWEKHGWR
                     GERFRGRGYDVWENLQQPVWVDIRVPRDAAPGIYTGSITVTASNTAPASIPVEIEVWD
                     FALPDGPTLENHFGGFERAASYYGIATDSEEYCRLEDRFIEMLVAHRINPPFPRRLLP
                     QLGENGSVTFSDELDRRITEYVAKYHVTNIEVPRAPFRDMLGAEREKAARFYQSWYAY
                     LERKGWADRAYLYMFDECLHKESYEKVRQIGPFVEAAEPRLRRLVVEAPYPRDASWGT
                     LDGNLDIWVSLFGYIDEASTQRVLKAGDEMWLYTALVQSIPKFHPDYENIKEPNLPHW
                     DPAAKAGRPPYWEMDFPLMSYRIAPWLNRRYDANGLLYWSSCYWFKKGTSTPQNPWDN
                     PTFGDHWNGEGFFVYPGADAGIDGLIASARLKNL"
     gene            complement(1633..>2096)
                     /locus_tag="ENN56_01400"
     CDS             complement(1633..>2096)
                     /locus_tag="ENN56_01400"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906883.1"
                     /note="catalyzes the oxidation of 3-isopropylmalate to
                     3-carboxy-4-methyl-2-oxopentanoate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDS74173.1"
                     /translation="FHEVGAKDYPDIVRDYAHVDAITMWMVKNPEWFDVIVTDNIFGD
                     IITDLGAMVQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGQGKINPIAAIAAAQMMV
                     DTLGEHDAAKAFERAIVHVVGEKMESMQAGRIGHTTSQVGDLIADAIAQGV"
BASE COUNT          416 a          630 c          577 g          473 t
ORIGIN      
        1 aggtttttca accgtgccga tgcaatcaat ccgtcgatac cggcgtccgc tcccgggtag
       61 acgaaaaacc cttcgccgtt ccagtggtcg ccgaacgttg ggttgtccca cggattctgc
      121 ggtgtgctcg ttcccttctt gaaccaatag caactcgacc agtagaggag accgttcgcg
      181 tcataccgtc gattcagcca cggagcgatg cggtaactca tgagcgggaa atccatctcc
      241 cagtacgggg gtcgaccggc tttcgctgcg ggatcccagt ggggcaaatt cggctctttg
      301 atgttttcat aatcgggatg gaacttcggt atcgactgga cgagtgccgt gtacagccac
      361 atctcgtcgc ccgctttgag cacgcgttgc gtactcgctt catcaatata tccgaagagc
      421 gagacccaga tatcgagatt cccgtcgagc gtgccccacg acgcgtcacg cgggtacggc
      481 gcttcgacaa cgagccgacg gagccgcggc tcggccgctt cgacaaacgg gccaatctga
      541 cgcaccttct cgtacgattc tttatgcaga cattcgtcga acatgtagag atatgcacgg
      601 tccgcccatc ccttccgttc gagatacgca taccacgact ggtagaatct cgcggctttc
      661 tcacgctccg cgccaagcat atcgcggaac ggcgcacggg gtacctcaat gttcgtgacg
      721 tgatacttcg caacgtactc cgtgatgcgt cggtcgagtt catcgctgaa ggtcacgctg
      781 ccattttcac ccagttgtgg aaggagccgt cgcgggaatg gtggattgat tcggtgtgcg
      841 acgagcatct cgatgaaacg gtcttcgagt cggcagtact cctccgaatc ggtggctatc
      901 ccgtagtacg acgccgctcg ctcgaaaccg ccgaagtggt tttcgagtgt cggaccgtcc
      961 ggcagcgcga aatcccatac ctcgatttcg acgggtatac tcgcaggagc tgtattcgac
     1021 gcagtgaccg ttattgatcc ggtgtatatg cccggcgcgg cgtcacgcgg tacacggata
     1081 tctacccata ccggctgttg cagattctcc cacacatcgt acccgcgacc gcggaatcgc
     1141 tcgccgcgcc acccgtgttt ttcccactgt ggacccgcaa caggctctcc ggtatacgga
     1201 ttcgtccgtg gcacgagcgc atccgcccac attccgggcg gcatgtccga ccgcggatcg
     1261 ggagtacgaa tcggcacgaa tacgtttcag aacacgtcga cgcagtccgc cggtaacgta
     1321 tcaccagacg cgcttcgcag ttcggaaacg gtcgcgtcga cttcgcgcag tgtcccgttt
     1381 ttcagtgata tgactacttg aaatgactcc gtctcgtttc gagcgcacga gatcgagact
     1441 gcattcgatc ccgagatccg gccgtcttgt cgaatattcg tactcgctga agctgcgacg
     1501 agccgaattg acggtgacga tgttgccatg ttttgtatcc tcaacgagtg cggcgaaaca
     1561 gaaaaagggc gatgaacggt tctcgacctt ggcttcatcg ccctttccaa aacgtatgat
     1621 ctgttcgcga cgttatacgc cctgtgcgat cgcgtccgcg ataaggtcgc cgacctgcga
     1681 tgtcgtgtga ccaatgcggc cggcttgcat cgattccatc ttttcgccga cgacatggac
     1741 gatcgcgcgt tcgaatgcct ttgccgcgtc gtgctcgccc aaggtgtcga ccatcatttg
     1801 cgccgctgcg atggcggcga tcgggttgat cttgccctga ccggtgtatt tcggcgcgct
     1861 tccaccgata ggctcgaaca ttgagacgcc ttcggggttg atattcccac cggcggcaac
     1921 acccatgcca ccctgaacca tcgcgccgag gtcggtgata atatcgccga agatattatc
     1981 ggtaacgatc acgtcgaacc attcggggtt tttcaccatc cacatggtaa tcgcatcgac
     2041 gtgagcgtaa tcgcgcacaa tgtccggata atccttcgcg ccgacttcgt ggaacg
//
LOCUS       DSCC01000121            1793 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044676, whole genome shotgun sequence.
ACCESSION   DSCC01000121 DSCC01000000
VERSION     DSCC01000121.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044676"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..547)
                     /locus_tag="ENN56_01405"
     CDS             complement(<1..547)
                     /locus_tag="ENN56_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDS74174.1"
                     /translation="MANEETKKKKSTKAPAKAKGKKAEKVAEAAPEKKTEAGIPTPRF
                     KNRYHESVVPQMIERFGYRNSMQVPRLAKIVINMGVGEGTQNTKLVDSAVEELQTITG
                     QRPNVRRARLSISNFRLREGTPVGAAVTLRRTRMWEFLDRLVTFSVPRIRDFQGLPRR
                     GCDGGGYYTFGLKEQLIFPEIT"
     gene            complement(566..910)
                     /locus_tag="ENN56_01410"
     CDS             complement(566..910)
                     /locus_tag="ENN56_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDS74175.1"
                     /translation="MDIVKGDLVEVISGEGRGQRRGEGQKGKVLWVDRDRGRVIVENV
                     NIVRHHEKPSQQNPQGGIVEREAPVSISNVLLVSPRLGRGVRVRHKRLEDGTRVRVCT
                     KTGEVIPPPTES"
     gene            complement(923..1291)
                     /locus_tag="ENN56_01415"
     CDS             complement(923..1291)
                     /locus_tag="ENN56_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDS74176.1"
                     /translation="MIQMQTSLTVADNTGAKRATVIKVLGGSRRRYATVGDEVIVAVK
                     EAIPGGTVKKGDVAKAVVVRVKRANRRADGSHIRFDDNAAVLIANDGEPRGTRIFGPV
                     ARELRDKRYMRIVSLASEVV"
     gene            complement(1311..1574)
                     /locus_tag="ENN56_01420"
     CDS             complement(1311..1574)
                     /locus_tag="ENN56_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDS74177.1"
                     /translation="MTTESRGHRKVRTGRVISGAADKTIVVAIDRLMQHPLYGRIVRR
                     RKKLHVHDENNDAHVGDFVEIMETRPMSKLKHWRLVRVIERAR"
     gene            complement(1571..1792)
                     /gene="rpmC"
                     /locus_tag="ENN56_01425"
     CDS             complement(1571..1792)
                     /gene="rpmC"
                     /locus_tag="ENN56_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDS74178.1"
                     /translation="MKKLTVEEVEQELEDARAEMWRLRFRAATEQLDNPMLTRHRRRD
                     IARMLTVLKEHRDPNHPTVLASREGGSES"
BASE COUNT          329 a          583 c          458 g          423 t
ORIGIN      
        1 acgtgatctc ggggaaaatc agctgttcct tgagcccgaa tgtgtagtac ccgcctccgt
       61 cacatccccg ccgcggcaat ccctggaagt cgcggattcg cggaacactg aacgtgacga
      121 gacgatcgag aaactcccac atgcgagtac gacgcagcgt caccgccgct cccaccggcg
      181 taccttcacg caggcggaaa ttcgaaatcg acaaccgggc gcgacgcaca ttcggccgct
      241 ggccggtaat cgtttgcagc tcttcgaccg ctgaatcgac caacttggtg ttctgcgtac
      301 cttcgccgac acccatgttg atgacgattt tcgcgagacg cgggacctgc atcgagttcc
      361 gatagccaaa ccgttcgatc atctgcggta ccacgctttc gtggtaacga ttcttgaatc
      421 gaggcgtcgg gataccggct tcggtcttct tttccggagc cgcttcagct accttttcgg
      481 ccttcttacc cttggccttc gcaggagcct tcgtgctctt cttcttcttt gtttcttcgt
      541 tggccatttg cttgcgctcc cgccgttacg attcggtcgg gggaggaatc acttctcccg
      601 ttttcgtgca cacacggact cgcgtaccat cttcgagccg cttgtgacgc acgcgcactc
      661 cacgtccgag acgaggactg accaggagca cgtttgaaat gcttaccggc gcctcacgct
      721 caacaatgcc gccctgtgga ttctgctggg acggcttctc gtggtgccgt acgatattga
      781 cattctcgac tatcacgcga ccgcgatccc gatcaaccca gagcaccttg cccttctgac
      841 cttctccgcg ccgctgtccg cgaccttcgc ccgaaatcac ttcgacgagg tcacccttga
      901 cgatatccat gacctttttc ccttacacca cttccgaggc aagcgacacg atacgcatgt
      961 accgcttgtc acggagttcg cgcgccaccg gtccaaaaat acgggtaccc cggggttcgc
     1021 cgtcattggc gattagcact gccgcgttgt cgtcgaaccg aatatgcgac ccatccgcgc
     1081 gacgatttgc gcgcttcacc cgaacgacga cggctttcgc tacatcgccc ttctttaccg
     1141 tgccacccgg aatcgcctct ttgactgcaa cgatcacttc gtcgccgacc gtcgcgtacc
     1201 ggcgacgcga gccgccgagt actttgatta ccgtcgcacg tttcgcgccg gtattatcgg
     1261 ctacggtgag agaagtctgc atttgaatca ttgacggtga cctttcctcg ttaccgcgcg
     1321 cgctcgatga cgcgtacgag ccgccaatgt tttagcttgc tcatcggacg cgtctccatg
     1381 atctcaacaa aatcgccaac gtgcgcatcg ttgttctcgt cgtgtacgtg caatttcttc
     1441 cgacgtctca caatgcgacc gtagagcgga tgctgcatga ggcggtcaat cgcgacgaca
     1501 attgtcttgt cagccgcacc actaatgacg cgtcccgtgc gaaccttgcg atgaccgcga
     1561 gattcagttg tcatgactca cttcctccct cgcggctcgc gagcaccgtc gggtgattgg
     1621 gatcgcgatg ttctttgagg accgtaagca ttcgcgcgat gtcacgccgg cggtgccggg
     1681 tcagcatcgg attatcgagc tgttccgtcg ccgcacgaaa ccgaagacgc cacatttcag
     1741 cgcgtgcgtc ctcaagctcc tgctcgactt cctctaccgt gagtttctta atg
//
LOCUS       DSCC01000122            2925 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023144, whole genome shotgun sequence.
ACCESSION   DSCC01000122 DSCC01000000
VERSION     DSCC01000122.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023144"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..215)
                     /locus_tag="ENN56_01430"
     CDS             complement(<1..215)
                     /locus_tag="ENN56_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDS74179.1"
                     /translation="MLNPSPIHATPSLEDALLLSASGTMLPIHRRILADTETPVSAFM
                     KIRNEDQYGFLLESVEGGEKIARYSFLG"
     gene            667..2088
                     /locus_tag="ENN56_01435"
     CDS             667..2088
                     /locus_tag="ENN56_01435"
                     /inference="COORDINATES: protein motif:HMM:PF04909.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74180.1"
                     /translation="MVSWCTIVSWCRTSLDSGGDMSELRQRLATELQNIPLIDTHEHF
                     PEERVRLRSLSDFFSYLHYVECDLTSAGSEPWHVLTQHASFDGRREEFFAFWPYVQHT
                     GYGQALKIMAFDLFGVEAITPETFETLNRNASMFPREGYYESLLRERANIDRVCRIVW
                     SGAPTHCDNDLLFPVVQFDHFANVLSRADIRDLENETNMPIHTLDDLLEALDESFDAR
                     EDEGMVGAKIFLAYKRTLFFDAPTTADAERIFNRATQAHGDTPLGYAEAKPLQDFIVR
                     RIVEQAIERDLPIQIHTGFQNDNANFVENTRPTHLTNLFMTYPAAKFELLHGGWPYTQ
                     EFVALAKVFPNVYPDMAWTYIIGSRMASRLLDDLLDSVPMNKILGFGGDYNFAEGAYA
                     HARIARRAIRHVLADRIERGDMTEDEALDVARLIMHDNAAKLYGFDTIEEMSSDTDVD
                     MDDDIDDDEDGFVDEAEDDIDDD"
     gene            1955..2905
                     /locus_tag="ENN56_01440"
     CDS             1955..2905
                     /locus_tag="ENN56_01440"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:PRK13111.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDS74181.1"
                     /translation="MPQNSTDSIPLKRCRAIPMSTWTTISMTTRTGSLTKPKTISMTT
                     ESGTGVNRITQAFSRARVDGRAAFIAYITCGDPTLERTKQLVLRFADAGVDIVEFGVP
                     FSDPMADGVANQEAANRALKNNVSMGDVLRTAGELRASGSELPVILFTYYNPVFAYGL
                     ERFAKDAARNGVDGVLLVDVPPEEASECTHLLADAGLATIFLVAPTTDETRLAEILAS
                     TTGFVYYVSRAGVTGERTDLASDVRDHVAKIRTQTDLPVAVGFGISTPDHVRRVAEVA
                     DGVIVGSAIVGRIGAGGDTDGMVDDVTSFVRELTRPLIER"
BASE COUNT          610 a          802 c          879 g          634 t
ORIGIN      
        1 ccgagaaatg agtatcgcgc gatcttctcg ccgccttcga cgctctcgag gagaaacccg
       61 tactgatcct cgttgcgaat cttcataaac gccgagaccg gcgtctcggt gtcagcgagg
      121 atgcgtcggt gaatgggcaa catcgtgcct gacgctgaaa gcaacaaagc gtcttcgagg
      181 ctcggtgttg cgtgtatggg tgacggattt aacatggttt atctcgatat gaaacgaaaa
      241 aggccgtgaa cacgacgtgc gtgttcacgg cctttgatct ggcacctata tatccggatc
      301 agccttcgac gacgcacgtc tccctatgtt ggctacgcca ataccagcgc caccaaccaa
      361 acgcgaagaa gtcgtgcgaa agagcggtct gcatgatccg cgattccgtt tttgagtgtg
      421 gtgtgtgccg cgaaaccgta tcgcgacagc gtgaagtata gagtcttttc gcgtcgttgt
      481 caacatactc gatcgatttc acgtggcgac gtgtttgttt cacgttccac ggcaggtgcc
      541 ggatgcggcg ttgagtgccg ttccactggt tccgaaggtg gtatcgtgct atgtttcaaa
      601 gacgtggcga cgaacgtaga gttcgtgtag cacgaagccc gtgtagcacg aagcgcgtgt
      661 agcacgatgg tatcgtggtg cacgatcgta tcgtggtgcc gcacctctct ggacagtgga
      721 ggagacatga gcgaattacg ccagcgactc gcgaccgagt tacaaaatat cccgttgatc
      781 gacacacatg aacactttcc ggaagaacgt gttcggttga gatcgttgtc cgatttcttc
      841 agctacctac actacgtgga gtgtgacctc acctcggcgg gctctgagcc atggcatgtt
      901 ctcacgcagc acgcctcgtt tgatggacgt cgcgaggaat tcttcgcgtt ctggccgtat
      961 gtccagcaca ccggttacgg gcaggcgttg aagatcatgg cgttcgatct gttcggcgtc
     1021 gaagcgatca cgccggagac gttcgaaacg ctcaaccgga acgcgtcaat gttcccgcgc
     1081 gaagggtatt acgaatcgct ccttcgagag cgtgcgaata tcgatcgcgt atgtcgaatc
     1141 gtgtggtccg gcgcgccgac tcactgcgac aacgacttgc tctttcccgt ggtccagttt
     1201 gaccatttcg ccaacgtgct gagtcgcgca gatatccgag acctcgaaaa cgaaacgaac
     1261 atgccgatac acacgctcga cgacctcctc gaagcgctcg acgagtcgtt cgacgcacgt
     1321 gaggacgagg gcatggtcgg cgcaaagata ttcctcgcgt acaagcggac gctcttcttc
     1381 gacgcgccga caaccgccga tgccgagcgc atcttcaacc gtgcgaccca agcgcacggc
     1441 gacacgccgc ttgggtacgc cgaggccaag ccactgcagg acttcattgt acggcgaatc
     1501 gtcgaacagg cgatcgagcg cgatctgccg attcagattc ataccggctt ccagaacgat
     1561 aacgcgaatt tcgtcgaaaa tacgcgacct acgcacctca cgaacctctt catgacctat
     1621 ccggcggcaa agttcgagtt gctgcacggc ggttggccgt atacgcagga attcgtcgcg
     1681 ttggcgaaag tcttcccgaa cgtgtatccg gatatggcgt ggacgtacat catcgggtcg
     1741 cgcatggcga gtcgtttgct tgacgatctg ctcgacagcg tgcccatgaa caagattctc
     1801 ggcttcggtg gcgattataa cttcgccgag ggagcatacg cccatgcacg gattgcgcgt
     1861 cgtgcaattc gtcacgttct cgccgaccgt atcgagcgcg gtgatatgac cgaagacgag
     1921 gcgcttgacg tcgcacggct gatcatgcac gacaatgccg caaaactcta cggattcgat
     1981 accattgaag agatgtcgag cgataccgat gtcgacatgg acgacgatat cgatgacgac
     2041 gaggacgggt tcgttgacga agccgaagac gatatcgatg acgactgaaa gtggaacagg
     2101 cgtgaaccgg ataactcagg cgttttcccg cgctcgggtc gacgggcgtg cggcgttcat
     2161 cgcgtatatc acctgtggcg acccgacgct cgagcggacg aagcagcttg ttctacggtt
     2221 tgccgatgca ggtgtcgata ttgttgaatt cggcgttccg ttcagcgatc ccatggcgga
     2281 tggcgtcgcg aatcaggaag cggcgaatcg tgcgttgaag aacaacgttt cgatgggcga
     2341 tgtgcttcgg acggcgggcg agttgcgcgc atccggctct gaactccccg tcatcctttt
     2401 cacgtattac aatccggtgt ttgcgtacgg gctcgagcgg tttgcgaagg acgccgcgcg
     2461 aaatggcgtt gatggggttc tgctggtgga tgtgccgccc gaggaagcat cggagtgcac
     2521 acatttgctc gcagacgccg gccttgcgac gatattcctc gttgctccta cgacggatga
     2581 aacgcgtctc gcggagattc tcgcgagcac taccggattc gtgtattacg tatcgcgtgc
     2641 cggtgtcacc ggtgagcgca cggatcttgc gtcggacgtt cgcgaccacg tcgcgaaaat
     2701 tcgcacgcag accgatctgc ccgttgcggt ggggttcggc atttcaacgc cggaccacgt
     2761 gcgcagggtt gccgaggtcg ctgacggtgt cattgtcggg agtgcaatcg tggggcgcat
     2821 cggcgccggt ggtgatactg acggaatggt ggacgatgtg acgagcttcg tccgcgaact
     2881 gacccgacca ctgattgagc ggtgaatgcc caccggttag agccc
//
LOCUS       DSCC01000123            2469 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028957, whole genome shotgun sequence.
ACCESSION   DSCC01000123 DSCC01000000
VERSION     DSCC01000123.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028957"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1475
                     /gene="glgP"
                     /locus_tag="ENN56_01445"
     CDS             <1..1475
                     /gene="glgP"
                     /locus_tag="ENN56_01445"
                     /inference="COORDINATES: protein motif:HMM:PF00343.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HDS74182.1"
                     /translation="NGVSKLHGKVAQSMWKSLFPGLPVDEVPITSITNGVHVRSWISN
                     DMAWLYNRYLGPTWMQRPADAEIWQKVKRIPDEELWRTHCRRRERLVNFSRKRLESQL
                     KRRKATRREVASASEVLNPDTLTIGFARRFATYKRATLLLRDPARLTKLLLDKERPLQ
                     FIFAGKAHPADSPGKEFMRQLVHFIRSSEELRRHLVFIEDYDINVARYMVQGVDVWLN
                     TPRRPLEASGTSGMKATLNGALNCSVLDGWWDEAWNDHGEKDVGWAIGSGEEYTDLEQ
                     DLEDDVESRALFNLLEQEIIPTFYEWNGLGLPRQWISRMKASMSNLCAEFNSSRQVSE
                     YTERFYIPASRRWTEFQSDEYKRAKELAAWKQRIRQYWGQVHFADIDHEKKKELAVGS
                     ELNVQANVFLGQLTPNDVAVQVYHGRIVKTDTKEDIDEIADGVAIDMAFDAKKDDNVY
                     HFTGTVPATGTGQHGFAIRVLPRHADLTNPFEMGLVVWSS"
     gene            1488..2327
                     /locus_tag="ENN56_01450"
     CDS             1488..2327
                     /locus_tag="ENN56_01450"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00440.21,HMM:PF09209.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDS74183.1"
                     /translation="MRVSRRGVDSDSPPFFIPHRVVPARHHWRSPLFPPLFVCAFQMY
                     ISNTRYSHSYVLARSRIMGHTRQRLLESACEIFAEQGYQAATVAEICERAEANIAAVN
                     YHFGSKEALYDEVLAYSRRVADTEVPLDGGLPANASSEQRLHAFIHAMLVRTLSEGRV
                     AWFQRMVAHERMKPESGMARKFRPYIQRNRAVLDAIIGEMGGGTLDEEIVHTCGSQVV
                     ALCNYWGYNRYARDRFTRKTSGAPDAIAKLARDTTRFARAGIRALIGIDGTHVDNTRN
                     NEK"
BASE COUNT          566 a          678 c          726 g          499 t
ORIGIN      
        1 ccaatggcgt gagcaagctg cacggaaagg tcgcgcagtc catgtggaag tcgctcttcc
       61 ccggactacc ggtcgatgag gtgccgatca catccattac caatggcgtg catgtgcggt
      121 cgtggatcag taacgacatg gcgtggctgt acaaccgcta cctcggcccg acgtggatgc
      181 agagacctgc agatgccgag atctggcaga aagtgaaaag gattccggac gaggaactct
      241 ggcgcacaca ctgccggcgg cgcgagcggc ttgtcaattt ctcgcggaag cggctcgaat
      301 cccagctgaa acgccgaaaa gcgacgcgaa gggaggtcgc ttcggcttcc gaggtgttaa
      361 atcctgatac tctgacgata ggttttgccc gtcggttcgc cacatataaa cgggctacgc
      421 ttcttctccg cgatccggca cgtctgacga agctgctctt ggataaagaa cgtccgcttc
      481 agttcatctt cgcaggaaag gcacatcccg ccgattcacc gggaaaagag ttcatgcgac
      541 agctcgttca tttcatacgt tcgtcggagg agcttcgtcg ccacctcgta ttcatcgagg
      601 actacgacat caacgtcgcg cgctatatgg tgcagggtgt cgacgtgtgg ctcaatactc
      661 cccgtcgtcc gctggaagcg agcggcacga gcggcatgaa agccacgctc aacggtgcgc
      721 tgaactgttc cgtgctcgac ggctggtggg atgaggcgtg gaacgaccat ggtgagaagg
      781 atgttggttg ggcgatcgga agcggcgaag agtacacgga cctcgagcag gatctcgaag
      841 acgatgtcga gtcacgcgcg ctgttcaatc tccttgaaca ggagattatc cccacgttct
      901 acgagtggaa cggactcggt cttccccgtc agtggatcag tcgaatgaag gcgagcatgt
      961 cgaacctttg cgccgaattc aactcgagcc gacaggtgag cgagtatacc gaaagattct
     1021 acataccggc ttcacggcgg tggaccgagt tccagagcga cgagtacaag cgcgccaagg
     1081 aactcgccgc gtggaaacaa cgcatccgtc agtattgggg acaggttcat ttcgcggata
     1141 tcgatcacga aaagaagaag gagctcgcgg tcggttccga gttgaacgtc caagccaatg
     1201 tgttcctcgg acaactcacg ccgaatgatg ttgcggtaca ggtgtatcac ggtcgtattg
     1261 ttaagacgga tacgaaagag gatattgacg aaatcgccga tggcgtcgca atcgacatgg
     1321 cgttcgacgc gaagaaggac gacaacgtct accactttac gggcaccgtt cctgcgaccg
     1381 gcaccggtca gcacggcttc gcgatccgcg tccttccgcg tcacgccgat ctgacgaatc
     1441 cgttcgaaat ggggctcgtc gtctggtcgt cataacaccg cgacaaaatg cgcgtctcac
     1501 ggcggggagt cgattccgat tccccgccgt tttttattcc ccaccgagtt gtccctgcac
     1561 ggcaccattg gcgttccccg ttgttcccgc cgcttttcgt atgcgcattt caaatgtata
     1621 tttcaaatac gcgatacagt cattcgtacg tcctcgcgag gagccgaatc atgggacaca
     1681 cgcgacagcg cctgcttgaa agcgcgtgcg agattttcgc cgagcagggc tatcaagccg
     1741 caacggtagc cgagatatgt gagcgcgccg aagcgaatat cgcggcggtc aattatcatt
     1801 tcgggtcgaa agaagcgctg tacgacgaag tgcttgcgta ttcgcgacgc gttgccgaca
     1861 ccgaagtccc actcgatggc ggtttgccgg ccaatgcctc gtcggaacaa cgactccacg
     1921 cgttcatcca cgcgatgctc gtgcgaacgc tgtcggaagg ccgcgtcgcg tggttccagc
     1981 gcatggtcgc gcacgagcgg atgaagccgg aatccggcat ggcgcggaag ttccgcccgt
     2041 acatccaacg gaatcgcgcg gtgctcgacg cgatcatcgg agaaatgggt ggcggcacgc
     2101 tcgacgagga gatcgttcac acgtgcggaa gtcaggttgt cgcgttatgc aactactggg
     2161 gctacaacag atacgcgcgc gatcgattca cgcgaaaaac gagtggtgct cctgatgcga
     2221 tcgcgaaact cgcgcgcgat acaactcggt ttgcgagagc cggaattcgc gcgctgatcg
     2281 gcatcgatgg gactcatgtc gataatacgc gaaataacga aaaatagatt ttcgcaccgt
     2341 gggagtaatt cgccctatag cgaaatgatc cgtaagtact tacgaaccaa atcactcgta
     2401 cggttctgag gtagagtcat gcaggaaatc cgaagactca ttcattggtt caaaccgtac
     2461 cgctggaag
//
LOCUS       DSCC01000124            5100 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1010881, whole genome shotgun sequence.
ACCESSION   DSCC01000124 DSCC01000000
VERSION     DSCC01000124.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1010881"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(229..678)
                     /locus_tag="ENN56_01455"
     CDS             complement(229..678)
                     /locus_tag="ENN56_01455"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDS74184.1"
                     /translation="MESIRVQITRLAHAADLPLPRYETDGAAGMDLRAAIAKPVSIAP
                     NETTRVPTGFAIAIPAGYEGQVRPRSGLAGRSKILVPNSPGTIDSDYRGEVQVLLTNI
                     GSAPFIVEPGMRIAQLVIAPVVRANWVEVDELPETVRGDGGFGHTGN"
     gene            complement(909..3125)
                     /gene="pnp"
                     /locus_tag="ENN56_01460"
     CDS             complement(909..3125)
                     /gene="pnp"
                     /locus_tag="ENN56_01460"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDS74185.1"
                     /translation="MISLRKRRTVVVRTYSLELDGKTITIEHGRMAAQADGAVVVRSG
                     DSMVLVTACMSPSVPNLPFFPLTVEYREKGYAAGKIPGSIFKREGKPSDEETLSARLI
                     DHQIRPLFPKAFQNEVQVIVSVLSHDQENDPDVLACVGASAALSISKIPFAGPYGTVR
                     VAADPETGNKIINPTYEQRENSDRDMVVAGNAESLLNIEGEAHEISEAEMLDSMTFAI
                     ESIKKLVDFQAQIVSEVGQPKVEVAEPEVNQELVKRVEDLAADRIRESLTIAEKSVRE
                     DAQEKIIDEAVAALADDYPTEQKEIADVISAIAKREMRNRILKEGTRIDGRDVKTVRD
                     ITIETGFLPRAHGSALFSRGQTQALASVTLGTRMDERLVDNLEGKSYKNYMLDYNFPP
                     FSTGEVKRIGPPSRRDIGHGALAERAIESVIPDDKSFPYTIRVVSDIMESNGSSSMAT
                     VCAASLALMDAGVPIKTAVAGINIGLITDKDSGNTAILTDLLGIEDANGDMDLKIAGT
                     RDGITAFQMDIKIGGIAIDTMREAFDRAHDGRLHVLNEMVKAIEAPRAQLSRFAPRII
                     TMNIDPSQIGAVIGPGGKIVREIQESTGATVEIEDDGTITISSLDGESGEAAAGRIKG
                     IVSVPEIGEKYHGTVKSIMPYGAFVEIMPGRDGLLHISEIEERRLDKVEDVLTVGDEI
                     EVIVMNVEANGKLRLSHKRLEASIEELKAEAEKSGSSSGGGGSRGGRGGSRGGGRR"
     gene            complement(3446..3715)
                     /locus_tag="ENN56_01465"
     CDS             complement(3446..3715)
                     /locus_tag="ENN56_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDS74186.1"
                     /translation="MAVTKERIAELIAEYQRHGKDTGSTEVQVALLSERIRNLTEHFR
                     THSKDHTSRRGLMKLVGQRRRLLNYLEKTNIEEYRKLISRLELRR"
     gene            complement(3828..4748)
                     /gene="truB"
                     /locus_tag="ENN56_01470"
     CDS             complement(3828..4748)
                     /gene="truB"
                     /locus_tag="ENN56_01470"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:PF09157.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDS74187.1"
                     /translation="MARRKPSDSAFPAGVVLIDKPLGITSHGVVSKLRKLFDTRRIGH
                     GGTLDPAATGVLVLFTGRATRLAPYLGGGAKVYLTTIRFGEATTTDDAEGDICATGSV
                     PNQTYAGISASAERFLGYIDQIPPAVSAVHHEGERAYRIARRGDSVELKPRRVHIDAI
                     DVLEWNPPDLVARVTCGTGTYIRSLARDWGEHLGTHAHVRTLRRERSGVFRIEDAITL
                     DEIADRLQSGASYRELVTDPGAALVEALGDQTVLNPDDTKRFVCGNDVRDDSGELDTV
                     AVLDASGTLLGVGERRSDGSLAPVVVLSAP"
     gene            complement(4768..>5100)
                     /gene="rbfA"
                     /locus_tag="ENN56_01475"
     CDS             complement(4768..>5100)
                     /gene="rbfA"
                     /locus_tag="ENN56_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDS74188.1"
                     /translation="LIRNEMRDPRVGYVTITRVDISDDLSLARVFVSELSTEERTSGE
                     SVEALRKAAGFLRRELGKRLRLRLTPELRFESDRSIEEGLRMTQLLDEIARKDEKHDD
                     DESDDGRT"
BASE COUNT          937 a         1522 c         1498 g         1143 t
ORIGIN      
        1 gtatcatccg cgccgaggtc ggcggcatcg agccgctcga atacgcagcc ggacgtctca
       61 agcgcggcca tgtgctcgcg gaaacgtgac ggcgagacgg ttgttgcgcc aagctctggt
      121 ccggacgcga ctgcgtgata ggtgagtatc gtgcgcgcca ttatcgatcc atgttaacgc
      181 aacaaaacgc cgagcgccga tacgactatc gagcgtcggc gatacagctc agttgccggt
      241 atggccgaat ccgccgtcac cgcgaacggt ttcgggaagc tcgtcgactt cgacccagtt
      301 cgctcgcaca acaggcgcga ttaccaactg tgcaattctc attcccggct cgacgataaa
      361 cggtgccgaa ccgatattcg tcaggagaac ctgtacctct ccgcgatagt cgctatcgat
      421 tgttcccggg ctattcggta caagaatctt cgatcgtccc gcaagaccgc tacgcggccg
      481 tacttgccct tcgtaaccgg cggggatcgc gatagcaaat cccgtcggga cgcgcgtcgt
      541 ttcgttcgga gctattgaga caggcttagc gattgccgct cgcagatcca taccggctgc
      601 gccatcggtt tcataccgcg gtaacggaag atctgccgca tgggcgaggc gcgtgatctg
      661 cacgcggata gattccatgt agtctacgcc ttcccgtgat acctcttcgc cggaccgatt
      721 agccggatga tagacaccaa gccgtaacag cctaagaaga tacggatatc ggtttgtgaa
      781 cgcgaagggg ttgttgaaaa cccttcgcga gacggtgtca ccgatagaac acgaaacagg
      841 ggcgcggcgc catatgattg tgacgccgtg cccctgcggt attcgtgagt cgattctacg
      901 cgggtgtgtt accgacgacc gccgccgcga ctaccgccac gaccgccgcg gctaccacca
      961 ccgccactgc tgctgccgga cttttcggcc tcggccttca gctcttcgat tgacgcctcg
     1021 agacgcttgt ggctcagacg cagcttcccg ttcgcttcga cgttcatcac aatcacttcg
     1081 atttcgtcgc cgacggtgag cacgtcttct accttgtcca gacgccgttc ttcgatttcg
     1141 gaaatgtgga ggagtccgtc acgacccggc atgatctcga cgaacgcacc gtagggcatg
     1201 atcgacttga ccgtgccgtg gtacttttcg ccgatttccg ggacggagac gattcccttg
     1261 atccgaccgg cggctgcctc gccgctctcg ccgtcgagtg aggaaatcgt aatcgttccg
     1321 tcgtcctcga tctcgaccgt cgcgccagtt gattcctgaa tctcgcgcac gattttgccg
     1381 cccgggccaa tcacggcgcc gatctgtgac ggatcgatgt tcatggtgat gatgcgcggt
     1441 gcgaaacggc tgagctgcgc acgcggcgct tcaatcgcct tgaccatctc gttgaggacg
     1501 tggagtcggc cgtcatgtgc gcggtcgaac gcttcacgca tcgtatcgat cgcgataccg
     1561 ccgatcttga tatccatctg aaacgcggtg ataccgtcgc gcgtcccggc gattttcagg
     1621 tccatgtcac cgtttgcatc ttcgataccg agcaggtcgg tgaggatcgc ggtgttgccg
     1681 ctgtccttat cggtgatcag accgatgttg atgccggcaa cggccgtctt gatcggcacg
     1741 cctgcatcca tgagcgcgag gctcgcggcg catacggttg ccatggaaga cgagccgttc
     1801 gactccataa tgtcggagac gacgcggatg gtgtacggga agctcttgtc atcgggaatc
     1861 accgattcga ttgcgcgctc ggcgagtgct ccgtggccga tatcgcggcg actcggtggt
     1921 ccgatacgct tgacttcgcc ggtggagaac ggcgggaagt tgtagtcgag catgtagttc
     1981 ttgtaggact tcccctcgag gttgtccaca agccgctcgt ccatccgcgt tcccaacgtc
     2041 accgacgcga gtgcttgcgt ctggccgcgg ctgaagagcg ccgatccgtg ggcacgtggg
     2101 aggaaaccgg tttcgatggt gatgtcgcgg acggttttca catcgcggcc atcgatacgg
     2161 gtgccttcct tcaggatgcg gttgcgcatt tcgcgtttcg caattgccga gataacgtcg
     2221 gcgatctctt tctgctcggt cgggtagtca tcggccagcg cggcgaccgc ttcgtcgatg
     2281 atcttttcct gtgcgtcttc gcgaacactc ttctcggcga tggtgaggct ctcgcgaatc
     2341 cggtcggcgg cgaggtcttc gacgcgcttg acgagctcct gattgacttc cggttcagct
     2401 acttcgactt tcggctgacc gacttcgctg acgatctgcg cctggaaatc gacgagtttc
     2461 ttgattgact cgattgcgaa cgtcatcgaa tcgagcatct cagcttcgct gatctcgtgc
     2521 gcttcgcctt cgatattcag caaagattcg gcattgcccg caacgaccat gtcgcgatcc
     2581 gagttttcgc gttgttcgta ggtcggattg atgatcttgt tgccggtttc gggatcggcg
     2641 gcaacgcgaa cggttccgta cggtccggcg aagggaatct tcgaaatgct gagtgccgcc
     2701 gatgcgccaa cgcacgcgag aacgtccgga tcgttttcct gatcgtgcga gagaacgctc
     2761 acgatgacct ggacttcgtt ctggaatgct tttgggaaca acgggcgaat ctggtgatcg
     2821 atgagccgcg ccgagagcgt ttcctcgtcg gacggcttgc cttcgcgctt gaagatactg
     2881 ccgggaatct tccccgcagc gtaccccttt tcgcggtatt cgaccgtcag cgggaagaag
     2941 ggaagattcg gtacactggg cgacatacat gccgtcacga gcaccattga atcaccactg
     3001 cgaacgacga ccgcgccatc ggcttgagca gccatgcggc catgttcgat ggtgattgtt
     3061 ttgccatcga gttctaaact gtacgtgcgg accactacgg tcctcctttt acggagcgaa
     3121 atcatgagtc atcgagagga atcatgggac gtcgcacgct gaaatcttcg cgtggtgtgc
     3181 tccctgtcgt ctgaagcgac gggccacgtg agattcagtc tactggaaac acgcaatcgc
     3241 cgtgagtcag caatcatgtt cccgatagac ggaaccgccg cggcacatac cgtcagttcg
     3301 ggtgctggac aacggtccgt cgcgtagtaa cacgcgccgt accgtatgat tcctccgatg
     3361 gtgaagtccg ccgacggctc cgtcatggac gtcggcggac tctgtctggc gggatgcgga
     3421 catcgttgtc cgcatcccaa aaacgttagc gacgcagctc caagcggctg atcagcttgc
     3481 ggtactcctc gatattcgtc ttctcgaggt agttcagcaa acgacgacgc tgaccgacga
     3541 gcttcatgag accacgtcgg ctcgtgtgat ccttgctgtg cgtccggaag tgctcggtca
     3601 gattccggat gcgctcggat aagagtgcta cctgaacctc ggtcgagccg gtgtccttac
     3661 cgtgccgctg atactcggcg atgagctctg cgatgcgctc ttttgttact gccacaccag
     3721 tctcctttct caccgaacga ccactatggg gccgttacaa cggcttccgg tgcggtggaa
     3781 gccgggcgat aatgtttcat cctcgcgata tcgtcgctgt gagccaatca cggcgcggag
     3841 aggaccacta cgggagcaag cgacccgtcg gatcgtcgtt ctcccactcc aagtaacgtt
     3901 ccggatgcgt cgagcaccgc tacggtgtcg agttctccgg aatcgtctcg aacatcattc
     3961 ccgcacacga accgtttcgt gtcatcggga ttcaacactg tctgatcacc gagcgcttcg
     4021 acaagcgctg cgcccggatc ggtaaccaat tcgcgatacg atgcgccact ctgaagtcgg
     4081 tccgcgatct cgtcgagggt aatcgcgtct tcgattcgga agacgccgga acgctcccga
     4141 cgcagcgtgc ggacgtgtgc gtgcgtcccg aggtgttcgc cccagtcacg cgcgagcgac
     4201 cgtatgtatg tgccagtgcc acaggttact cttgcgacga ggtccggcgg attccactcg
     4261 agcacgtcaa tcgcgtcgat atgaacgcgt cgcggtttga gttcgaccga atcaccgcgc
     4321 cgggcgatgc gatatgcgcg ttcgccttca tgatgcaccg cgctgaccgc cggcggtatc
     4381 tgatcgatgt agccaagaaa tcgttcggcg cttgcggata ttcccgcgta tgtttgattc
     4441 ggcacggaac cggtcgcgca gatatcgcct tcggcgtcgt ctgtcgtcgt cgcttcgccg
     4501 aaccggatcg ttgtcagata caccttcgcg ccaccaccga gatacggtgc aagacgcgtc
     4561 gctcgaccgg taaacagcac gagtacgcct gttgcagccg gatcgagcgt gccgccgtgt
     4621 ccgatccttc gcgtatcgaa gagcttgcga agcttcgaga cgacgccgtg cgacgtgatt
     4681 cccaacggtt tatcgataag aacgactccc gccgggaacg cgctatctga tggtttcctg
     4741 cgggccatgc gtacggatat ctatcgttca ggtacgacca tcgtcggatt cgtcgtcgtc
     4801 gtgtttttcg tctttgcggg cgatctcgtc gaggagctgc gtcatacgaa gcccttcttc
     4861 gatcgatcgg tcggactcaa accgaagctc gggtgtgagc ctgaggcgaa gccgtttccc
     4921 caactcgcgt cggaggaagc ctgccgcttt gcggagcgcc tcgacgcttt cgccgcttgt
     4981 ccgctcttcc gtcgacagtt cgcttacgaa tacccgcgcg aggctcaggt cgtcggatat
     5041 atcgacccgg gtgatggtga catatcccac tcgggggtca cgcatctcgt ttcggatcaa
//
LOCUS       DSCC01000125            2096 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036145, whole genome shotgun sequence.
ACCESSION   DSCC01000125 DSCC01000000
VERSION     DSCC01000125.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036145"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="ENN56_01480"
     CDS             complement(<1..1904)
                     /locus_tag="ENN56_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74189.1"
                     /translation="MRTAHDTPWRNQKRIVFPVRCIAAYDDQRNRWVSRWGEPIVIGS
                     GAWLENGAASESDDVRMTAALCRDAWSPMIEPDASLPNIVWVKESPTLVAVDFSNDTI
                     EVAVDVASTLGLTFKDDAIHGPDVVKRGFTALLSLAGRFALAARADSSPDTDTIQRNG
                     IDAVVDAFLALDANMRHAIVETLQSPQIDAGHIFGQFLRTVTDEKRRDQWGEDPRAWR
                     RRWITWLIGQSRVDLPYDRDTINEILLDPTLDPDDARLALYQTVRGYDIETEKANAER
                     IGKSVSWSGQELIFGRMSRAFHNQALLFANARYVTVTPKVAETANTCVDAALTWPQLR
                     PAADRLRTMMRADRETPLTEMAGALDHLEEQTLRHQRELSQAALEERRVQVERAESDP
                     DTDHRSGKRMDEIMRQAEAILSSAGNLRHALLEAPRRPAAYLIMSQRPSPTGGHLLAK
                     LNEGEEPFLGKAANLRRLVPQGGDRVYASPDYSWLEYANHWIEAIPLFIKERIRVIDG
                     VETAETVIDQEGMEESFRESMADPWARNLRRTETSGWCAIARLLLDENADPRTADLGL
                     QADIARAWNVKQTGCADDVALLSAVIALTAESTAASISASVERDPTTDRPTAVRAILF
                     GDTETLHPVWT"
BASE COUNT          390 a          580 c          657 g          469 t
ORIGIN      
        1 gtccagacgg ggtgtagcgt ttccgtatcg ccgaaaagta tcgcgcggac ggcggttggt
       61 cggtcggtgg tgggatcgcg ttcgaccgat gcgctgatcg aagcggcagt cgattccgcg
      121 gtgagcgcga tcaccgccga aagcaacgcc acatcgtcgg cgcaaccggt ttgtttcacg
      181 ttccacgcac gggcgatatc tgcttgaagg ccgaggtcgg cggtgcgtgg gtcggcgttc
      241 tcgtcgagta gtaggcgcgc tatggcgcac cacccggatg tctcagtgcg acgaagattc
      301 cgcgcccacg gatccgccat cgattcgcgg aaggactctt ccataccctc ttggtcaatc
      361 acggtctcgg ctgtttcgac accgtcgatg actcgaatac gctctttgat aaacagcgga
      421 atcgcttcga tccagtgatt cgcgtattcg agccatgaat agtcgggcga cgcgtaaact
      481 cgatcaccgc cctgcggcac gagccggcga aggtttgcgg ccttcccgag gaagggttct
      541 tcgccttcat tcagcttggc gagtagatgc ccccccgtcg gcgatgggcg ctggctcatg
      601 atgagatacg ctgcgggccg tcggggcgct tcgagaagtg catgccgtag gttgcccgct
      661 gaactgagta tggcttcggc ttggcgcatg atctcgtcca tgcgtttgcc ggatcgatgg
      721 tccgtatcgg gatcggactc ggctcgttct acctgtacgc ggcgttcttc gagggcggct
      781 tgcgagagtt cacgttggtg tcggagtgtc tgttcttcga gatgatcgag cgctcccgcc
      841 atctccgtga gcggtgtctc gcggtcggcg cgcatcatgg tgcgaagccg gtcagcggcc
      901 ggacggagtt gcggccacgt aagagcggcg tcgacacacg tattcgcggt ttccgcgact
      961 ttcggtgtga cggttacgta ccgcgcattt gcgaaaagta atgcctggtt gtgaaacgcg
     1021 cgactcatgc gaccgaagat gagttcttgc cccgaccacg aaacggactt gccgatacgt
     1081 tcggcgttcg ctttttccgt ttcgatgtcg tatccgcgaa cagtctggta taacgcaagg
     1141 cgggcgtcgt cgggatcgag tgtcggatcg agaagtatct cgttgatggt gtcgcggtca
     1201 tacgggagat cgacgcgcga ctgtccgatg agccacgtaa tccagcgacg acgccacgcg
     1261 cgaggatctt cgccccattg atcgcgccgt ttttcgtcgg ttaccgtgcg aaggaactga
     1321 ccaaagatgt gaccggcatc gatctgaggc gactgcaacg tctcaacgat cgcgtggcgc
     1381 atgttggcat cgagagcgag gaacgcgtcg acaaccgcgt cgattccgtt acgctgaatg
     1441 gtatcggtat cgggcgagga atctgctcgt gccgcgagtg cgaaacgccc ggcgaggctg
     1501 agtagagctg taaatcctcg cttgactacg tccggaccgt gtatcgcgtc gtctttgaac
     1561 gtgagaccga gcgtcgatgc cacatctaca gcaacttcaa tggtgtcgtt agagaaatcg
     1621 accgccacga gtgttggaga ttcttttacc catacaatat tggggagcga tgcgtccggt
     1681 tcaatcattg gagaccacgc gtcgcgacac agtgctgcag tcattcgtac gtcgtcggat
     1741 tcacttgcgg caccattttc gagccacgca ccggaaccga tgacgatcgg ctcaccccat
     1801 cgactcaccc atctgttgcg ctggtcatcg tacgcggcga tacagcgtac cgggaagacg
     1861 attcgtttct gatttcgcca tggggtgtcg tgagcggttc gcattcgtgt tcctcagttt
     1921 cgtagagcgg cgtctcggtt cgcgatcgca gtaggtgcga tctcgacgaa cggacgcgtg
     1981 gagaaacctg tagcatcgcc caccgaagtg acccgagtga ttcggtttcg gaggtaaacc
     2041 gtgtagagtt gacacgttcg ggcggcaaat tgttaagata ccacgtccta tcggac
//
LOCUS       DSCC01000126            2424 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029671, whole genome shotgun sequence.
ACCESSION   DSCC01000126 DSCC01000000
VERSION     DSCC01000126.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029671"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            315..1124
                     /locus_tag="ENN56_01485"
     CDS             315..1124
                     /locus_tag="ENN56_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDS74190.1"
                     /translation="MPVIMDKVELSPNVYRIEVDAPLVATRRRPGQFVIVRASDESER
                     IPLTIVDSDPVAGTITLIYQALGEGTRFLAKLEMGDEIRDIAGPLGRATDLSYFGKVV
                     CVGGGIGVAPLYPIVRGLKELANEVTVVMGARNEGLLILEEEMREVADEVVIATDDGS
                     RGFHGFVSAALQERIDAGYDPKLCVTIGPLPMMRSVAEVTRPYGIHTIVSLNPVMVDG
                     TGMCGGCRVTVKGERKFACVDGPEFDGHAVDYAELASRQRMYRDHEQCAIR"
     gene            1170..>2424
                     /gene="gltA"
                     /locus_tag="ENN56_01490"
     CDS             1170..>2424
                     /gene="gltA"
                     /locus_tag="ENN56_01490"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDS74191.1"
                     /translation="MSSPTEARRRTPQPKAPRNIMPERPAQERAKDFREVATGFTAEM
                     AVAEALRCLQCKKPLCIDGCPVGIDIPAFIDYVAENDFAGGARRIKVDTNLPAICGRV
                     CPQEKQCEERCIVGKKHKPVSIGALERFVADWERESGSVSVPEIAEATGKKVAVVGSG
                     PAGLTAAGELARRGHAVTVFEALHEPGGVLVYGIPEFRLPKEIVRREVDSLRQMGVEI
                     QLNALIGQAITVDELFNEGYDAVFIGTGAGLPHFLDIPGENLNGIYTANEFLTRVNLM
                     KAYRFPEYHTPVYLGKRIAVFGGGNTAMDSARTSLRLGPDSVKLIYRRSEAEMPARLE
                     EIHHGRDEGVEFVTLAAPLEFIGDEKGWLKAVRIIHMELGEPDASGRRRPIPIEGSEE
                     LLEIDTAIIAIGNGPNQLIPNTTPGL"
BASE COUNT          570 a          601 c          720 g          533 t
ORIGIN      
        1 acccatgtca tagggtgtca tattgagtat agagatcttc gaggtgatag tcaagagtgg
       61 agtgagcggc attcgcgagt cgcgattcga atgtgtcgtt tctgcgcatg tttgtgccat
      121 taccgcgcca tttcgcgtcc acgtgtcttc tctcgtaggt cgaagatacc gaaccaccga
      181 tcattcggct taatggcacg gtacgtgcac gcaaaacacc gcccggtaaa cggggagttt
      241 tcggtacaga aatcggtttt caccacccac cgatgtgatt tcgtcgaaag aaacggaaaa
      301 agagaggcag agcaatgccc gtcataatgg acaaggttga actctcacca aatgtatacc
      361 gcatcgaagt cgatgcccct ttggtggcaa cgcggcgacg tcccggacag tttgtgatcg
      421 ttcgtgcgag cgatgaatca gagcgaatcc cgttgacgat tgtagacagt gatcctgtcg
      481 ccggaacgat taccctcatc taccaagctc tcggtgaggg caccagattc ctcgcaaaac
      541 tcgagatggg tgacgagatt cgcgatattg caggacctct cggacgtgcg acggatttat
      601 cgtatttcgg aaaagtggtg tgtgtcggcg gtggtatcgg tgttgcgccg ttgtatccca
      661 ttgttcgggg tctcaaggaa ctggcgaacg aagttaccgt ggtaatgggt gcgaggaatg
      721 aaggtttgct cattctggaa gaagagatgc gcgaagtcgc cgatgaagtg gttatcgcga
      781 ccgatgatgg tagtcgcggc ttccacggat tcgtcagtgc cgcgcttcaa gaacgtatag
      841 acgcgggata cgatccaaaa ctgtgcgtga cgatcggacc gcttccaatg atgcgcagcg
      901 ttgctgaagt aacccgtccg tacggcatcc acacgatcgt gagtctcaac ccggtcatgg
      961 tcgacggaac gggtatgtgc ggtggatgcc gcgttaccgt caaaggcgaa cggaaatttg
     1021 cgtgcgtaga cggaccggaa ttcgatggcc acgcggtcga ttacgccgaa ttggcgagcc
     1081 gtcagcgcat gtaccgcgac cacgaacagt gcgcaatccg ttagaatagc taattgcaca
     1141 acgtaacgcg agatagatag aggtattcaa tgagctcacc gactgaagcg cgtaggcgta
     1201 ccccgcaacc gaaggcacct cgcaacatca tgccggagcg tccggcacaa gagcgtgcaa
     1261 aagactttcg cgaagttgct actggattca cggcggaaat ggctgtagcg gaagcgctgc
     1321 gctgccttca gtgcaaaaag ccactctgca tcgacggatg tccggttggt atcgacattc
     1381 cggccttcat cgactacgtc gctgaaaacg atttcgcggg tggcgctcgt cgcattaagg
     1441 tcgacacaaa cctgcctgcg atatgtggac gtgtgtgtcc tcaagagaaa caatgcgaag
     1501 aacggtgtat cgtcgggaag aagcacaaac cggttagtat cggcgcactt gagcgattcg
     1561 tcgccgattg ggaacgtgag agcggttctg tatcggtgcc agagatcgca gaagcgaccg
     1621 gaaagaaggt tgccgtcgtt ggttctggtc cagccggact tacggcggcc ggcgaacttg
     1681 cgcgacgcgg ccatgcggta acggtcttcg aagcgcttca tgaaccgggc ggtgtgcttg
     1741 tctatggtat tccagagttc cgtcttccga aagagatcgt ccgacgtgaa gtggattcac
     1801 tgcgtcagat gggtgtagaa atccaactca atgctcttat cggacaggcg attactgtcg
     1861 atgaactgtt caacgaaggg tacgacgctg tttttattgg cacgggcgct ggtttgcctc
     1921 atttccttga tataccgggc gaaaatctga acgggatata cactgcgaac gagtttctta
     1981 cgcgcgttaa tctgatgaaa gcgtaccgtt tccccgagta ccacacgcct gtctacctcg
     2041 ggaaacgtat tgccgtattc ggtggcggaa atacggcaat ggactcggcg cgtacgagtc
     2101 ttcgactggg tcccgattcg gtaaagttga tttatcgccg ttcagaagcg gagatgcctg
     2161 cgcgtctcga agagattcat catgggcgag acgaaggcgt agaattcgta acgctcgccg
     2221 cgccgctcga gttcatcggc gacgaaaaag gatggctaaa agcggttcgc atcatccata
     2281 tggagcttgg tgagccagat gcgagcggac gccgccgccc gattccgatt gagggtagcg
     2341 aagaactact tgagatcgac acggcgatta tcgcgatcgg gaacggaccg aaccaattga
     2401 tcccgaatac gactccgggt ctcg
//
LOCUS       DSCC01000127            1747 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046240, whole genome shotgun sequence.
ACCESSION   DSCC01000127 DSCC01000000
VERSION     DSCC01000127.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046240"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..576
                     /locus_tag="ENN56_01495"
     CDS             <1..576
                     /locus_tag="ENN56_01495"
                     /inference="COORDINATES: protein motif:HMM:PF00850.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HDS74192.1"
                     /translation="PPGHHALHGSAMGFCLFNNVAIGARYARQKHGIERVLIVDWDFH
                     HGNGTQETFWRDGSVMCFSVHCSPAYPYSGFVDQRGEGPGEGLIVNAPLAPGAGHYEY
                     ADAFENTLKPAAERFEPELVLISAGFDAHRSDPLSLMRIDETDFREFTDRVRDIADRF
                     AGGRIVSVLEGGYNVDTLASSAEEHVRRLLT"
     gene            576..1031
                     /gene="rimI"
                     /locus_tag="ENN56_01500"
     CDS             576..1031
                     /gene="rimI"
                     /locus_tag="ENN56_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase"
                     /protein_id="HDS74193.1"
                     /translation="MTLRPFRAEDCEQVERLESVLFRVPYDEPWSAAIIGAYARYSHL
                     HGWVAEDDGVIIGYLLATIVLDEANIDNLGVDPTRQRTGVARRLLEHAIEQFREHGVR
                     HVWLEVAVPNEAARAFYLAAGFEQVDQRRNYYHGSHGISADALILTKAL"
     gene            1028..>1747
                     /locus_tag="ENN56_01505"
     CDS             1028..>1747
                     /locus_tag="ENN56_01505"
                     /inference="COORDINATES: protein motif:HMM:PF01048.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74194.1"
                     /translation="MNGTGSGMNHVSTARSSRNIVPDSSAAARVTTAASWLAERGVVD
                     PMGMIVLGSGWAPLIERVEVLGRWPYSEIPGLPSCSVKGHPGDLVYGRWDGVPLIVFV
                     GRWHAYEGRTPEDLALPPRIAKALGARWMLGTNAAGGIDSNLRVGDLVAVTDDLSFWA
                     TGRAMQVSGIVGDTPQHSGAPYSQRLVDALLRAAEDVGISLHRGTLAMMTGANYEAAA
                     EIAMLRFAGASVVSMSTLPEVR"
BASE COUNT          322 a          516 c          569 g          340 t
ORIGIN      
        1 ccgccggggc atcacgcgct ccacggtagc gccatgggat tctgcctgtt caacaacgtt
       61 gccatcggcg cacgatacgc gcgtcagaaa cacggcatag agcgcgtgct catcgttgat
      121 tgggatttcc atcacggaaa cggtacgcag gagacgttct ggcgcgatgg ctcggtgatg
      181 tgtttcagcg tccactgctc acccgcgtac ccgtatagcg gatttgtgga tcagcgaggc
      241 gaaggccccg gcgaggggct catcgtgaat gcgccgcttg ctcccggcgc cggacactat
      301 gagtacgcag acgccttcga aaacacactc aagcccgcag cggaacgatt cgaaccggag
      361 ctcgtgctca tcagcgccgg gttcgatgcc catcgaagtg acccactttc gttgatgcgc
      421 atcgatgaga cggatttccg cgagttcacc gatcgcgtgc gcgatatcgc cgaccgtttc
      481 gccggcggcc ggatcgtgag cgtgctggaa ggcggataca acgtcgatac actcgcatca
      541 tccgccgaag agcacgtccg gcggctgctc acgtgatgac acttcgccca tttcgcgcgg
      601 aggactgcga gcaggtcgaa cggctcgagt cggtgctgtt tcgcgtgccg tacgacgagc
      661 cgtggtccgc cgcgataatc ggcgcctacg cgcggtattc ccacctccac ggctgggtcg
      721 ctgaagacga cggcgtcatt atcggttatt tgttggcaac gatcgtgctt gacgaggcga
      781 acatcgacaa tctcggtgtc gacccgacgc gacagcgaac aggcgtcgcg cggaggctcc
      841 tcgagcatgc gatagagcaa ttccgggagc acggcgttcg gcatgtatgg ctcgaggtcg
      901 ccgtcccgaa tgaagccgcg cgcgcctttt atctcgcagc gggattcgag caggtggacc
      961 aaaggcgaaa ttattatcac ggttcgcacg gcatatcggc cgacgcgctc atactcacga
     1021 aagcactatg aacggcacgg gtagcggaat gaaccacgtg agtacggcac gttcgtctcg
     1081 aaatatcgta cccgactcga gcgcggcggc tcgtgttacc actgcggcgt cgtggctcgc
     1141 ggaacgtggc gtcgtcgatc cgatggggat gatcgtgctc ggcagcggtt gggcgccact
     1201 catcgaacgc gtcgaggttc ttggtcggtg gccatattcc gaaatccccg gtcttccatc
     1261 gtgctcggtg aaggggcatc ccggcgacct cgtctatggc cgatgggacg gtgttccgct
     1321 tatcgttttc gtcgggcgat ggcacgcgta tgaagggcga acaccggagg atctggcgtt
     1381 gccgccgcgt atcgcgaagg cactcggtgc acggtggatg ctcggaacga acgcggccgg
     1441 tggaatcgat tcaaatctgc gcgtcggcga tctcgtcgcc gtgaccgacg atctcagctt
     1501 ttgggcaacc ggtcgcgcga tgcaggtaag cggtattgtc ggcgataccc cgcagcatag
     1561 cggcgccccg tactcacaac gactggtcga cgcgttgctc cgtgcggcgg aagacgtagg
     1621 gatttcctta catcggggga ctctcgcgat gatgaccggc gcgaactacg aggctgcggc
     1681 ggagatcgcg atgttacgtt tcgccggtgc gtccgtggtg agcatgtcga cgctgccgga
     1741 agtccgg
//
LOCUS       DSCC01000128            2806 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024469, whole genome shotgun sequence.
ACCESSION   DSCC01000128 DSCC01000000
VERSION     DSCC01000128.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024469"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..670)
                     /gene="hrcA"
                     /locus_tag="ENN56_01510"
     CDS             complement(<1..670)
                     /gene="hrcA"
                     /locus_tag="ENN56_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDS74195.1"
                     /translation="MRSLNERERAVLTAIIEEYTESAEPVGSRTLSRKYFGDLSPATI
                     RNTMYDLEELGLLVQPHTSAGREPTAAGYRLYLNSLMKQPRLSLHDRRELDQLLSEER
                     ATRDEDTVLLHVAQALANISHLISLAFFPSFDDAILEKIDLLPISDNRVLTILQIRAG
                     TVDTVMITLPEPVRPDQISETVRALNERLAGETIGRIRRTIGERLRGLSYGDQAVINV
                     FLNEG"
     gene            complement(837..1691)
                     /locus_tag="ENN56_01515"
     CDS             complement(837..1691)
                     /locus_tag="ENN56_01515"
                     /inference="COORDINATES: protein motif:HMM:PF04951.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74196.1"
                     /translation="MTPETTAGFSRNDGTTSQNEGDDMRIYLCTDMEGISGISYPDEF
                     MTDGPRYEYGRTLLTGDVAAAVEGLKAGGATKIVVLDGHGGGNNFIFDKLPAGAEYIC
                     GRGGSPRALPFLDESYDAVGMIGQHAMAGTPDATWPHTQSSASWLGMWVNGREIGEIG
                     QFAITAGGYGLPLILFTGDRAACAEVRELVGDQVETVAVKHAASATRAKCLALDDARH
                     AIYDAAKHSLGRIATAQPLRVDMPMEVKIRFNATKFADDREKNGADRVDLLTVRRITN
                     DPYGVYAV"
     gene            1893..2753
                     /locus_tag="ENN56_01520"
     CDS             1893..2753
                     /locus_tag="ENN56_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74197.1"
                     /translation="MSISPLFRGITLFAMIAVLFVWTGTQAAIITTVSGERIVCEIIE
                     ERPEYYIIDHDGYRRYLLKELIRSVELDPPVVRDPNPMRHHSLGVIGGYYRNVQSQSQ
                     ELAGVRVSYAFPFSEHVAIEANAHFGAGTVNESAGSKLDPGDMLWSGGSLGALWNTRI
                     GPTFWFVESSLGYFVIDHTIDPAAKSKRLIVNRALEIIPAGQTGDFEQNPDNGFGLVF
                     GGGFMYPIFRHLAVETRATIIMIDSRMRDVVTYDPIAGDALYSTGKHVNTYELFTEMA
                     QFQVALRWTF"
BASE COUNT          588 a          801 c          762 g          655 t
ORIGIN      
        1 caccttcatt cagaaatacg ttgataaccg cttgatcgcc gtacgaaagt ccgcgcagtc
       61 gctccccgat cgttctccga atacgcccga tcgtctcacc ggcaagtctt tcattcagtg
      121 cgcggactgt ttccgagatc tggtccggac gaaccggttc tggcagcgtt atcatgaccg
      181 tatcgacggt tcccgcacga atttgcagaa tcgtgaggac gcggttatcg gaaatcggaa
      241 gcaggtcgat cttttcgagt atcgcgtcat cgaagctcgg aaagaacgcg agacttatca
      301 ggtgggagat atttgcaagc gcctgagcaa catggagaag gacggtatcc tcgtcgcgcg
      361 tcgcacgttc ttcactgagt agctgatcga gttcgcgccg gtcgtgcagt gataagcgcg
      421 gctgtttcat aagggagttt agatacagcc ggtatccagc agcagtcggt tcccgaccgg
      481 cgctcgtgtg cggctgcacg agaagcccaa gctcctcgag atcgtacatc gtgttgcgaa
      541 ttgtcgccgg actcaggtcg ccgaaatact ttcgtgatag cgtacgcgag cctaccggtt
      601 cagccgattc cgtgtactcc tcaattatgg cagtgagtac cgcacgttcg cgttcgttga
      661 gcgagcgcat atctgccctc ccgccgcata caggtccatc acagaggact caatgtgtcg
      721 cggcgatttt tggcactctc gacaggtgag tgctaatgcc ctgatgtcaa gtataggacg
      781 gccgggagaa ttggtcaagt tacgcgtctt gacacccgcc gtactggccg atgccgtcac
      841 acggcgtaca caccatacgg atcgttcgtg atacgccgta cggtgagaag atcgactcgg
      901 tccgcaccgt tcttctctct atcgtcggcg aacttggtcg cgttgaaccg aatctttacc
      961 tccatcggca tatcgacgcg gagtggttgc gcggtcgcga tacgtccgag cgagtgtttc
     1021 gcagcgtcat agatggcgtg acgcgcatcg tcgagcgcaa ggcatttcgc gcgtgtagcg
     1081 gaagccgcat gctttaccgc cacggtttcg acctgatctc ccacgagttc gcgcacttct
     1141 gcacacgctg cgcgatcacc ggtaaacaaa atgagcggca atccgtatcc gcccgcagtg
     1201 atggcgaact gaccgatttc gccgatctcc cgcccgttta cccacatgcc gagccaactc
     1261 gccgacgatt gtgtgtgcgg ccaggttgcg tccggcgttc ccgccattgc gtgctgccca
     1321 atcatgccga ccgcgtcata cgattcgtcg agaaatggaa gcgctcgcgg actgccaccg
     1381 cgtccacaga tatattcggc accggcggga agtttgtcaa aaatgaagtt gtttcccccg
     1441 ccatgcccat ccaatacaac gattttcgtc gctccaccgg ctttaagccc ttccacagcc
     1501 gccgcgacgt caccggtaag tagtgtgcgt ccgtactcgt atcgcggccc gtcggtcatg
     1561 aactcgtctg gatacgagat accggaaatc ccttccatat cagtgcacag atagattcgc
     1621 atgtcgtctc cttcgttctg cgatgtcgta ccgtcgttac gcgagaaacc tgccgtcgtt
     1681 tccggtgtta tatttcatac attcggaaat ctaccgcgga aaagattcca caagtcctac
     1741 catcgggatt tcagaataca tcgtcgcaca tgaagtgtag gaatagaccg acgcggtcca
     1801 aggccgtttc ggttgttctc acatacgcat tttgttaccg tggactgccg ttaccgcgca
     1861 gtgatcgatc ctcgacttat ctggagcgta tcatgagtat ttcgccgcta tttcgcggta
     1921 tcacgttatt cgcgatgatt gccgtactgt tcgtatggac gggaacacag gccgcgatta
     1981 tcacgacggt ttcgggcgag cgaatcgtct gcgagatcat tgaagagcga ccggaatact
     2041 acattatcga tcacgacggc tatcgacggt atctcctcaa agaactcata cgcagcgttg
     2101 agctcgatcc tccggtcgtg cgcgatccga atccaatgcg ccatcactcc ctcggcgtga
     2161 tcggcggtta ctatcgaaac gttcagtcgc agagtcagga gctggcggga gttcgggttt
     2221 cttacgcatt tccgttcagc gagcacgtcg ctattgaagc aaacgctcat ttcggcgcgg
     2281 gcaccgtaaa cgaaagtgcg gggagtaagt tggatccggg agacatgctg tggagtggcg
     2341 gctcgctcgg tgcactgtgg aatacgcgaa tcggcccgac gttctggttt gtcgaatcct
     2401 cattgggata tttcgttatc gatcacacga tcgatcccgc agcgaaaagt aaacgcctta
     2461 tcgtcaatcg ggcattggag atcataccgg ccggccagac gggtgatttc gaacagaatc
     2521 cggataacgg cttcgggctc gtgttcggcg gcggattcat gtacccgatt ttcaggcatc
     2581 tcgccgttga gactcgcgca acaatcatca tgattgattc gcgcatgcgc gacgtcgtca
     2641 cgtacgaccc aatcgcggga gacgcgttgt attccaccgg aaaacacgta aacacgtacg
     2701 agctgttcac cgagatggcc cagttccaag tcgcgctccg atggacgttc taatccgtca
     2761 ctcccgctcg cgtttttcgt agccttcgcc gacgacgtta tagtca
//
LOCUS       DSCC01000129            2202 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033787, whole genome shotgun sequence.
ACCESSION   DSCC01000129 DSCC01000000
VERSION     DSCC01000129.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033787"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(749..1090)
                     /locus_tag="ENN56_01525"
     CDS             complement(749..1090)
                     /locus_tag="ENN56_01525"
                     /inference="COORDINATES: protein motif:HMM:PF12831.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS74198.1"
                     /translation="MPPGPPINVDDGYGNPSGKNCSSHVPSSSIVFIASSNSNAESRS
                     YLSRIQNAANASRAASFSHIAASSCRLSRTIMQLGQAAGMSLRGEKQQPPRGVFSFHS
                     ETWGEGRRPSP"
     gene            1101..1745
                     /locus_tag="ENN56_01530"
     CDS             1101..1745
                     /locus_tag="ENN56_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74199.1"
                     /translation="MVALALAAQWLRLGGSVIAVPLQYANIRDVADAMLELYNIDLAD
                     YRRKFAYIQFDLAVDITEKNSDDTVNANLLKPEAWDAALTKAEELITKSDQGTLVLST
                     ALNLLLFSPTYREALLEKLKTLLSQDRSRTHICTVSTSVFENDIESLEEASDNVLSVR
                     MQRPMNLYLTINRMRNVSFVKDEQRVPIESSVLKKMKQEAERTRTQLIPKLKKI"
     gene            complement(1778..>2202)
                     /locus_tag="ENN56_01535"
     CDS             complement(1778..>2202)
                     /locus_tag="ENN56_01535"
                     /inference="COORDINATES: protein motif:HMM:PF12831.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS74200.1"
                     /translation="WERVWTAPMLGVRESRRIVGEYVLTQNDIEAGLSQQQHADIVAI
                     ADHALDLHAEHGGCRELSEPYGIPFRCLVPKGFHNLLIASRAASFSHIAASSCRLSRT
                     IMQLGQAAGMSLRGAKRQPSGVSSLRSEAWHEGRRPSP"
BASE COUNT          519 a          601 c          653 g          429 t
ORIGIN      
        1 agacgtcgtt gtcgctggga cgccggaagt cgttgcggca catccgacct cgcacaccgg
       61 tcgcgtgctg cgcgaggcgt atgggcgtac gcagcagaca ccaattgatg ccggcgaacc
      121 ggcagagttc gttacggaag cggacatact cgaggtcgtc aatgacgaac ctgacgagga
      181 aagcgccgag gagactccgg tcgacgatta tgaccgttcg gattcgcgcg acgcgccgtg
      241 gcgggtcgat ccgcacacat ggcacaccga tccgcagcga ccgcggctcg atgggaagct
      301 cccgctgtgg gagcctgacg cgctgacggt tgttgtcgag atggcagaag aactcggtgt
      361 gacggatatt cggtggaaca acaaaatcat cgtgagcgga ttcattccaa atatcggccg
      421 ttggatagag ctgcgaacgg cggactcgca ttcgatgcgc attcgactgc tggtaccgaa
      481 ggggacgttt gacggtcgga agctacggaa gcaactcggt atcgaaccgc tcgacaaagt
      541 ggatgactcg cccatcggcg gaaagcggca gcgcgttcgc atgaacgggc gcggcaagga
      601 ttggactcgt gtggatatcg ctgtgctgtg gaagcgcgag atcgacacac cgggattctg
      661 aacgttcctc cgcgaggcgt atgatgcgtt cgtcgcggcg aataatctcg actgaaaacg
      721 cgaactcgaa gctggcgctt cgagttcgtt aaggcgaagg gcgacgccct tcgccccacg
      781 tctccgaatg aaacgagaag acgccgcgcg gcggttgctg tttttctcca cgtagcgaca
      841 ttcccgccgc ttgccccaat tgcatgatcg tccgagacaa acggcacgac gatgccgcga
      901 tatgtgagaa cgatgcggcg cgcgacgcat ttgcagcgtt ttgtattctt gacaagtaag
      961 aacgtgattc agcgtttgaa ttggaggatg caatgaacac gatggaactg gacggaacgt
     1021 ggctggagca gttctttccg gaaggatttc cgtacccgtc gtccacgtta ataggtggac
     1081 ccggcggcac cggaaaacct ttggtcgcgc ttgccctcgc cgctcaatgg ctccgactcg
     1141 gtgggagtgt aatcgccgtt ccgctgcagt atgcaaacat acgagacgta gctgacgcga
     1201 tgctcgagct atacaacatt gatctcgccg actatcgacg aaaattcgcg tatatacagt
     1261 ttgatttggc ggttgatatc accgaaaaga acagcgacga cacggtcaat gccaacctat
     1321 tgaaaccgga agcctgggac gcggcactaa cgaaagccga ggagttgatt acgaagagcg
     1381 atcagggaac gttagttctt tctaccgcgc tgaatctgct cctattctcg ccgacatacc
     1441 gagaggcttt gctggagaag ctgaaaacgc tcctcagtca ggatcgatcg cgaacgcaca
     1501 tctgtacggt gagcacctca gtatttgaaa acgatatcga atcactcgaa gaggcgtcgg
     1561 acaacgtcct atcggtaaga atgcagcgac cgatgaacct gtatctcaca atcaacagga
     1621 tgcgcaacgt ttccttcgtg aaagacgaac aacgggtgcc tatcgagtcg tccgtgctca
     1681 aaaagatgaa acaggaagcg gagagaaccc gtacacaact cattccaaag ctgaaaaaga
     1741 tatagtcggc gaactcggag ctggcgcttc gagttcgtta aggcgaaggg cgacgccctt
     1801 cgtgccacgc ctccgagcga agcgaggaga cgcccgacgg ttgccgtttc gctccacgta
     1861 gcgacattcc cgccgcttgc cccaattgca tgatcgtccg agacaaacgg cacgacgacg
     1921 ccgcgatatg tgagaacgat gcggcgcgcg acgcaatgag caggttgtga aatccttttg
     1981 gaacgagaca tcggaatggt attccgtacg gctcgctgag ctctcgacac ccgccgtgtt
     2041 cggcgtgcag gtccagcgcg tgatccgcaa tcgcaacgat atccgcgtgt tgctgttggc
     2101 tgagacccgc ctcaatgtcg ttctgtgtga ggacgtattc gccgacgata cgacgcgact
     2161 cacgcacacc aagcatcggc gcagtccaca cgcgttccca tg
//
LOCUS       DSCC01000130            1522 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055691, whole genome shotgun sequence.
ACCESSION   DSCC01000130 DSCC01000000
VERSION     DSCC01000130.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055691"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..479
                     /locus_tag="ENN56_01540"
     CDS             <1..479
                     /locus_tag="ENN56_01540"
                     /inference="COORDINATES: protein motif:HMM:PF08241.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDS74201.1"
                     /translation="RAKLAPEDNALDVGTGTGVVAMHVAPKVRRVVGVDRSLPMLRVA
                     RDRVATSGSRNVLMEFGDLLDLPIRPDSIDVAFCSLVLRLVAKPSDAVTNIARTLRPG
                     GRIVVCDTLRTTDKETGINPAHLSAWLKNAKLTNISLDVLGDSETGKYLVGVGHRE"
     gene            complement(483..>1522)
                     /locus_tag="ENN56_01545"
     CDS             complement(483..>1522)
                     /locus_tag="ENN56_01545"
                     /inference="COORDINATES: protein motif:HMM:PF13671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS74202.1"
                     /translation="SGWADELAALDARLAGDEYGEEFLIAILAVAAARHIFPNSAQPD
                     GGARFARLLSHRAGYSPDITESAVALARYCHVPAEDNPVISKLLVASLIVDMRCVART
                     AEVFGVDTARFRQRCCALDCWDGPFEPSGIPEEASPHVRRRVVEAVVERVVPVDEVEA
                     YARSLASDTIGHMIVTVGIPGAGKTTWVERVLGSVPIVSTDRLRGVLLGDATDQSRND
                     MIISRSIAAAESIAASGRTVVFDATNTNHRGRERIRRALRRSGARSTMVYFDTSLDEA
                     LRRNMIRPRAVPDEVIRKFYRRLEAPRPDEADTTVIVSNVWDQPRIRRYGCELRIRDA
                     IPVEDVGAYLR"
BASE COUNT          331 a          490 c          423 g          278 t
ORIGIN      
        1 accgagcgaa gctcgcaccg gaagacaacg cactcgatgt cggtacgggc accggagttg
       61 tcgcgatgca cgtggcgccg aaagttcgac gcgtcgtggg tgttgatcga tcgcttccga
      121 tgctccgtgt cgcccgcgac cgcgtcgcaa cgagcggttc gcgaaacgtg ctcatggaat
      181 tcggcgactt actcgacctg ccgatccggc cggactctat cgatgtagcg ttctgctcac
      241 tcgtgctgcg tctcgtagcc aaaccgtccg acgcggtgac aaatatcgcg cgtacactcc
      301 gacccggagg ccgcatcgtc gtctgcgaca cgcttcgcac aaccgacaag gaaacgggaa
      361 tcaacccagc tcatttgagc gcatggttga aaaacgccaa actgaccaat atcagcctcg
      421 acgttctcgg ggacagcgaa accggcaagt atctcgtcgg tgtcggtcat cgcgagtgat
      481 tttcatcgga gatacgcacc gacatcctcg acgggaatcg cgtcgcgaat acgtagttca
      541 catccatatc gtcggatgcg cggctgatcc cacacgttcg acacaatgac cgtcgtgtct
      601 gcctcgtctg gacgcggcgc ttctaatcga cggtagaact tccgaatcac ctcatccgga
      661 acagcacgtg gccgaatcat gttacggcgt agcgcttcgt cgagacttgt atcgaagtag
      721 accatcgtcg aacgtgcgcc ggaccgtcgc aaagcgcgtc gaatacgctc gcgaccacgg
      781 tgattcgtgt tggttgcatc gaacacaacc gtcctacccg acgcggcgat cgattccgcc
      841 gcagcgatac tccggctgat gatcatatcg ttccggctct gatcggttgc gtcgcctaac
      901 aacacaccgc gcagacgatc ggtcgaaaca ataggcacgc taccgagcac gcgctccacc
      961 cacgtcgtct ttcccgcgcc cggtatccca accgtaacga tcatgtgacc gatcgtgtcc
     1021 gatgccagac ttcgtgcata cgcttcgacc tcgtcgactg gcacaactcg ctcgacaacc
     1081 gcctcgacca cgcgtcgacg cacatggggc gacgcctcct ccggaattcc cgacggctca
     1141 aacggcccgt cccaacaatc gagcgcgcag caccgctgac gaaaccgggc ggtgtcgaca
     1201 ccgaatactt cggcagtccg tgcgacgcac cgcatatcca cgatgagcga cgcaaccagg
     1261 agcttcgaaa tcaccggatt atcttcagcc ggaacgtggc aataccgggc gagtgcgacc
     1321 gcggattcgg tgatgtccgg cgagtaaccg gcgcgatgag agaggaggcg cgcaaaacga
     1381 gctccgccat cgggctgtgc ggaattggga aagatgtgac gcgcagcggc tactgcaagt
     1441 atcgctatga gaaactcttc gccgtattca tctccggcaa ggcgggcatc caacgccgca
     1501 agttcatcgg cccatccaga cc
//
LOCUS       DSCC01000131            1507 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1056404, whole genome shotgun sequence.
ACCESSION   DSCC01000131 DSCC01000000
VERSION     DSCC01000131.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1056404"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1507)
                     /locus_tag="ENN56_01550"
     CDS             complement(<1..>1507)
                     /locus_tag="ENN56_01550"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00905.20,HMM:PF03717.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDS74203.1"
                     /translation="ITIRLIDIQVIRSDRFAQRAAGQQERQVILDARRGRISDRNGVD
                     LAVSLPATSVSINPRKVRRATGLARQISAITGEPSDAILRRINRNDRSFAWVARNIEP
                     EVAERIRALDPDAMMLLSGTRRVHPLAGLSGQLLGHVDIDNVGGDGIELLYNDALSGR
                     SGWIRTLVDARGDRVPNTAAPIQQAVNGGNVALTIDADYQAIVEHELSLAIEATGATS
                     GQAILLEASTGAVRAMANVPLFNPDVYRAVDPSVRRNRVISDPYEPGSTFKIVTASAV
                     LSEQVITPTDSIDCLNGSIEITGQRIRDTHPNGKIPFREVFSRSSNVGTIIAAQRLSS
                     DDYYRYIRRFGFGAETGVGLPGESKGILAPTTRWSGRSHATIAIGQEIGVTALQMASA
                     YAALANDGWLMRPYVVDRVLAHDGSVAQHATPLRVRQVVPESVAHQMVEMLTSVVDEG
                     TGKTARIAGLRVAGKTGTAQIARADGRGYEIGAYTASFAGFLPDMEPRLVCV"
BASE COUNT          321 a          434 c          420 g          332 t
ORIGIN      
        1 acgcatacaa gccttggctc catatctgga agaaaaccgg caaacgaagc tgtatacgcg
       61 ccgatctcat acccccggcc gtctgctcgc gcgatctgag ccgtgccagt ttttccggcg
      121 acgcgcaaac cggcgatccg agctgttttt ccggttcctt cgtcaacgac actggtcagc
      181 atttcgacca tttggtgtgc gacgctttcc ggaacaacct gacggacgcg aagcggcgtt
      241 gcgtgttggg cgaccgagcc gtcgtgagca agcacgcggt cgacaacata cgggcgcatg
      301 agccaaccat cgttggcgag ggcggcgtac gcggaagcca tctgtagtgc ggttacaccg
      361 atttcctgac caattgcgat cgtcgcgtga ctgcgtcccg accaccgcgt tgtcggtgcg
      421 aggattccct tggattcgcc cggtaagccg acgccggttt cggcgccaaa tccgaagcgg
      481 cgaatatatc ggtagtagtc atcgctgctg aggcgctgag cggcgattat cgtgccgacg
      541 ttagacgaac gcgagaatac ctcgcgaaac ggtatcttac cgttcggatg tgtgtcgcga
      601 atccgctgac cggtaatttc tatgcttccg ttcaggcagt cgatactgtc cgtaggagta
      661 atcacctgtt cggaaagaac agcactcgcg gtgacgattt tgaacgtcga tccgggttca
      721 tacgggtccg aaatcacacg atttcggcga acactcggat caacagctcg gtacacatcg
      781 gggttaaaca aagggacatt cgccatcgct ctgaccgcac cggtcgatgc ttccagcaat
      841 atcgcctgtc cgcttgtagc tccagttgct tcgatcgcga gggagagctc gtgttcaacg
      901 attgcctggt aatccgcatc aatcgtgagc gctacattcc cgccgttaac ggcttgttgg
      961 attggtgcag ctgtgttcgg aacccgatct ccgcgggcgt caacaagcgt tcgtatccaa
     1021 ccgctacggc ctgaaagcgc gtcgttgtac agtagctcga taccatcgcc accaacgttg
     1081 tcaatgtcta cgtgtccgag gagttgcccc gaaagtccgg cgagtggatg aactcggcgt
     1141 gttccggaga gaagcatcat cgcatcggga tctaacgcgc gaatccgctc cgcgacttcc
     1201 ggttcgatgt ttcgtgcaac ccacgcaaac gatctatcat tccgattgat ccgtcgaaga
     1261 atagcatctg aaggctctcc ggtaatcgca ctgatctggc gcgccaaacc ggttgctctt
     1321 cgaaccttcc gtggattgat cgaaacggaa gtagccggaa ggctcacggc gagatcaacg
     1381 ccgtttcgat cagaaatgcg tccacgacga gcgtcgagga tcacctgacg ttcttgttgg
     1441 ccggccgccc gttgagcaaa acggtcactc cgaatcacct ggatatcgat aagacgtatt
     1501 gttatcc
//
LOCUS       DSCC01000132            1919 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040747, whole genome shotgun sequence.
ACCESSION   DSCC01000132 DSCC01000000
VERSION     DSCC01000132.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040747"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(73..918)
                     /locus_tag="ENN56_01555"
     CDS             complement(73..918)
                     /locus_tag="ENN56_01555"
                     /inference="COORDINATES: protein motif:HMM:PF00950.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDS74204.1"
                     /translation="MGDFADYLSSPFVLRAFVALILAAVNAALSGAFASFRNSTFLIS
                     GASHAALGGAALVIVLEVNGIVAGVDPLIGAAVFAVVLALLAAHASARGQHADVDVTI
                     GVGFALAMAVAVLLISLVPESASRVWGLLMGDLLLVSSGELMLVSALTLVVALTFFVF
                     RREFLFVTFDMEGAKAFGIRATRYNYVLFALIGLSAAILVKSIGAILVFAMLSAPAAT
                     ALRIAKSVGTAMWLAFGFALLAGILGILVSYATGFSVSALSAFFAAGAYFVVRAVQWE
                     SGPDE"
     gene            complement(911..1720)
                     /locus_tag="ENN56_01560"
     CDS             complement(911..1720)
                     /locus_tag="ENN56_01560"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDS74205.1"
                     /translation="MREILPKTETVVEAEHLSFVYRETEIEAIEDMSFRLAPAQFSAI
                     MGPNGSGKSTLLRLMIGLLQPTQGSIRLMGEDPARSPDVVQRLVGYVPQYESINMRLP
                     VRNRDIVGLGVASRTNRRLGASEVQDRAQAALEAVGMASMSDRRYSSLSGGQRQRVLI
                     ARALAVDPHVLVLDEPFAAMDLANQRRIAESMQRLVREKGISVITVVHNVNTLVHFID
                     RVILINRILIAEGHPDEVLRREPLQQAYGATVPIIVCEEGFPHPIMEDSHG"
BASE COUNT          454 a          569 c          534 g          362 t
ORIGIN      
        1 cactacacgt cagtcgtgag atgggcgcct acatgatttc gtcggtcgaa aaccgtaatt
       61 gtgtgcagta actcattcgt caggtccgct ttcccactga actgcgcgaa cgacgaagta
      121 ggcgccggcg gcgaagaacg cactgagcgc actaaccgag aacccggtag cgtacgacac
      181 gagtatcccg agaataccgg caagtagcgc aaacccaaac gcgagccaca tggcggtacc
      241 gaccgatttc gcgatgcgta acgccgtcgc tgcgggggcg cttagcatgg caaacacgag
      301 gatcgcgccg atacttttta cgagaatcgc cgccgaaaga ccgataagcg cgaagagcac
      361 ataattgtag cgagtcgcac gaatcccgaa cgctttcgcg ccttccatat caaacgtgac
      421 gaacaggaac tcacgccgga agacgaaaaa cgtgagcgca acgacaagcg tgagcgcgct
      481 gacgagcatt aactccccac tactcaccag taacaggtcg cccatgagga gtccccatac
      541 gcggctcgcc gattccggta caagagaaat gagcaacact gcaacagcca tcgccaacgc
      601 gaatccgacg ccgatggtaa cgtcgacgtc cgcgtgctga ccgcgtgcgc tcgcgtgcgc
      661 tgcgaggagt gcgaggacaa ccgcgaatac cgccgctccg atgagcggat cgaccccggc
      721 aacgatgccg tttacctcca acacaatcac gagtgccgcg ccgcccagcg cggcgtgtga
      781 cgcaccggag atgagaaacg tggagttgcg aaaactcgca aacgcaccac tgagcgccgc
      841 gttcactgcg gcaagtatca gtgccacaaa cgctcgtagg acgaacggcg aagacagata
      901 gtccgcaaaa tcacccatgc gagtcttcca taatcggatg cgggaaacct tcttcgcaga
      961 cgatgattgg gacagtcgca ccgtacgcct gctgtagtgg ttcgcgacgg agcacttcgt
     1021 ccggatgccc ttcggcgatg agaatgcggt ttatgagaat gacacggtcg ataaaatgaa
     1081 cgagtgtatt cacattatgc acgacggtga tgacgctgat gcccttttcg cgaacaagcc
     1141 gttgcatcga ttccgcgata cgtcgttgat ttgcgagatc catcgcggcg aacggctcgt
     1201 cgagaaccag cacgtgcgga tcgactgcca gcgcgcgtgc gatcagtacg cgttgccgtt
     1261 gaccgccgct gaggctgctg taacggcgat cgctcattga tgccataccg acggcttcga
     1321 gtgctgcctg tgcgcggtcc tgcacttcac tcgcgccgag tcgcctattt gtgcgcgaag
     1381 cgacaccgag tccaacgatg tcgcggttgc gcaccggcaa tcgcatgttg atcgattcgt
     1441 actgtggcac atacccgaca agccgctgga ccacgtccgg agaccgtgcc gggtcttcac
     1501 ccatgagacg aatactgcct tgcgtgggct ggagtaggcc aatcatcaag cggagtaatg
     1561 ttgatttccc ggagccgttc ggacccataa tcgccgagaa ttgagccggt gcaaggcgaa
     1621 acgacatgtc ctcgatcgct tcgatttccg tttcgcggta cacgaaactc agatgttccg
     1681 cttcgacgac agtctctgtt ttcggtagta tctcacgcac gtatcacttc ctaccttgcg
     1741 aacgggactg attcttctgt cgaatcgtga cgagcaccaa caggaaaaac actccgatga
     1801 gcgcgatacc tacgcttatc aacatgaatg aaccaccgtt ttcattgctc ggcggtgctc
     1861 cgtctcccaa gtcgcgcacg atcagggccg cgttgaagta cgcctgtgcg atgtacgaa
//
LOCUS       DSCC01000133            1919 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040741, whole genome shotgun sequence.
ACCESSION   DSCC01000133 DSCC01000000
VERSION     DSCC01000133.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040741"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="ENN56_01565"
     CDS             complement(<1..1036)
                     /locus_tag="ENN56_01565"
                     /inference="COORDINATES: protein motif:HMM:PF13177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74206.1"
                     /translation="MAPLSTDTPHTEVFARLMRAKQNGRLGHSLLLTGGGGHGAYVLA
                     LRLAEQFLGIDIKREWSALFNHPDFLCISPLPPKTEMEKRGKDKPDPVIESLLRDPHE
                     PINVGSNWRITADQGRRLVQWTALSPWQSDEKFVLIAEAEKTKEDAADIMLKTLEEPQ
                     PGVTIVLVTMRPQDLLPTVRSRCHETSVPPLDTENIAALLIERGAREEAAHSIARLAQ
                     GDFWQASALSAGDTNELRRDAAKMIVVALDPKERVADIMGDIKAVTDGMSPTEISEYV
                     RWMIWWLRDLILAVEGLQPPRPEVEPALDWARKVGFERLTEWLVEADRSYEMLGRNVT
                     PEAVVAALTLY"
     gene            complement(1095..>1919)
                     /locus_tag="ENN56_01570"
     CDS             complement(1095..>1919)
                     /locus_tag="ENN56_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS74207.1"
                     /translation="VYDNMAFGLKLRKFPKKDIDTRVRDAAEILGIGALLNRKPKALS
                     GGQRQRVAVGRAIVRHPKVFLFDEPLSNLDAKFRVAMRTEISKLHARLEATMIYVTHD
                     QTEAMTMGDRIVVMDDGIIQQVDPPVTVYEKPANRFVAGFIGSPAMNFIEGRVVSQED
                     SFVFDADGFSILLSDGIRERLSGFTGEEITLGVRPEWLFSGSNLDAAKDPARITAKVD
                     LVEPMGSETYLYLSVGDATIIARVDPHVLPEIGDEITIGIDMERAHFFDTSGATIV"
BASE COUNT          390 a          582 c          530 g          417 t
ORIGIN      
        1 gatacaacgt gagcgccgcc accaccgcct ccggtgtaac gtttcgccca agcatttcat
       61 aactacgatc agcttctacg agccactcgg tcaggcgttc aaatccgact ttccgtgccc
      121 aatcaagcgc cggttcgacc tctgggcgcg gcggctgaag tccctcaacc gcgagtatca
      181 ggtcacgcag ccaccagatc atccagcgta cgtactcgct gatctcggta ggtgacatcc
      241 cgtcggtaac ggccttgata tcacccatga tgtcagcgac tcgttccttc gggtccaacg
      301 cgacaacaat cattttcgca gcgtcgcgac gcagttcgtt cgtatcgccg gccgatagcg
      361 cgcttgcctg ccagaaatca ccttgtgcga gtcgcgcaat cgagtgcgcg gcttcctcac
      421 gtgcgcctcg ttcgatgagt aaggccgcaa tgttttcagt atcgagcggc ggaacactgg
      481 tttcgtggca gcgcgaacgc accgtgggta gaagatcctg cggtcgcatc gtaaccaaaa
      541 cgatcgtcac acccggctgt ggctcttcga gcgttttgag cataatgtcc gctgcgtctt
      601 cttttgtctt ttcagcttca gcgatgagca cgaacttttc atcgctctgc cacggggaca
      661 atgcggtcca ctgaaccaag cggcgccctt gatcggccgt gatacgccag tttgagccga
      721 cgttgatcgg ttcatgcgga tcacggagca aggattcgat aacgggatcc ggtttatcct
      781 tccctcgttt ttccatttcg gtcttcggcg gcaacggcga gatacagagg aagtccggat
      841 gattgaacag cgccgaccat tcgcgtttta tgtcgatgcc aaggaactgc tcggcgagtc
      901 gcaatgcgag gacatacgcc ccatgtccgc caccgccagt gagaagtagc gagtgcccca
      961 gtcgaccgtt ttgtttcgct cgcatgagcc gagcaaacac ctcggtatgc ggtgtatcgg
     1021 tactcaacgg cgccatagag gcgactcccg tcgaaggaaa tgttacggct cgcgcacaga
     1081 ctacacgatg cagtctacac gattgttgca ccggacgtgt cgaagaagtg cgcccgctcc
     1141 atatcgatgc cgatcgtaat ctcgtcacca atctccggta gcacgtgcgg atcgacgcgt
     1201 gcgatgatgg ttgcgtcgcc gacactcagg tataggtacg tttcgcttcc catcggctcc
     1261 acgagatcaa ccttcgccgt gattcgtgcg gggtcctttg cagcgtcgag gttcgaaccg
     1321 ctgaacagcc actccggacg cacgccgagg gttatctcct caccggtaaa cccgctcagc
     1381 cgctcgcgga ttccgtcgga gagcaagatg ctgaacccat ccgcatcgaa tacgaacgag
     1441 tcttcttgtg aaacgacgcg accctcgatg aagttcatag ccggcgagcc aatgaatccg
     1501 gcaacgaacc ggttcgcagg cttttcgtag acggttaccg gcggatcgac ctgttgaatg
     1561 attccgtcgt ccataacaac gatgcggtcg cccatggtca tggcttcggt ctgatcgtgg
     1621 gtgacataaa tcatcgtcgc ttcgagtcgg gcgtggagct tgctgatctc cgttcgcatg
     1681 gccacgcgga acttcgcatc gaggttcgag agcggctcgt cgaaaaggaa caccttcgga
     1741 tgccgcacaa tggcgcgtcc gaccgcgacg cgttgacgct gaccaccgga gagcgccttc
     1801 ggcttccggt tgagcagcgc gccaatgccg agaatctccg cggcatcacg tacgcgcgtg
     1861 tcgatgtcct tcttcgggaa cttccgcagc ttcaaaccaa acgccatatt gtcgtagac
//
LOCUS       DSCC01000134            1583 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052887, whole genome shotgun sequence.
ACCESSION   DSCC01000134 DSCC01000000
VERSION     DSCC01000134.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052887"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="ENN56_01575"
     CDS             complement(<1..1343)
                     /locus_tag="ENN56_01575"
                     /inference="COORDINATES: protein motif:HMM:PF09261.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74208.1"
                     /translation="MLIPLWSEPRSKETCMPDITAKIAALSRDLDTAHGSVKERIERI
                     SSELSFAWRLRELHPDKATEWEQLIGRALDQVNAAFSGNACADLPAAVEKAEQILEPI
                     GAVAKDYTVWNVGHGHIDMNWMWSWPETVSVTIDTFRTVLALMDAYDDFHYSQSQTSV
                     YDLLRIHDPKLLDRIAERVKEGRWEITASHWVEADKNLTGGESFCRHLLYTRAFMHEY
                     FGLEPSDVPIDWEPDTFGHAHTIPSFLARGGVKRYYHCRSGIDQKRPAVFWWQAPDKS
                     RVLVYHEIPGSWYNGRVEPGTTNSMIDFCKETGLNAWMNVYGVGDHGGGPTREDIERC
                     HEMNAWPIYPTFVLSSTKPFYELLEENGDRWATLDQELNFEFQGCYTTQTSIKKANRF
                     GENQLYESEAAATLASAQTGMAYPAERFREGWINTLFSQFHDILPGSGVRDTRDY"
BASE COUNT          328 a          469 c          429 g          357 t
ORIGIN      
        1 cagtagtcgc gcgtatctcg aacacccgat cccggcagga tgtcgtggaa ttggctgaag
       61 agcgtgttga tccaaccttc gcggaagcgc tccgccggat atgccatgcc ggtctgcgcc
      121 gatgcgagcg tcgcggcggc ttccgattcg tagagttggt tttcgccgaa acgattcgcc
      181 ttcttgatgc tcgtctgcgt cgtgtaacag ccttgaaact cgaaattcag ctcctgatcg
      241 agcgtcgccc agcgatcgcc attttcctcg agtagttcgt aaaatggctt cgtgctggaa
      301 aggacaaacg tcggatagat cggccacgcg ttcatttcat gacaccgctc gatatcttca
      361 cgagtcggac cgccgccgtg atccccaaca ccatagacgt tcatccatgc gttcaaaccg
      421 gtttctttgc agaaatcgat catcgagttc gtcgtgcccg gctcgacgcg gccgttatac
      481 cacgatccgg gaatctcatg atagacgagc acgcgcgact tgtccggtgc ttgccaccaa
      541 aacaccgcgg ggcgcttctg atcgattcca ctccggcaat gatagtaccg cttcacaccg
      601 ccgcgcgcga ggaatgacgg aatcgtgtgc gcgtggccga aggtatccgg ctcccaatcg
      661 atgggaacat cagacggctc gagaccaaaa tactcgtgca tgaacgcccg tgtgtagagc
      721 agatgccgac agaagctctc gccgccggtg aggttcttat ccgcttcgac ccagtggctt
      781 gcggtgatct cccaacgacc ttccttgaca cgctcggcaa ttcgatcgag cagtttcgga
      841 tcgtgaatgc ggagcaggtc gtacacactc gtctgactct gcgagtagtg gaagtcgtca
      901 tacgcatcca tcaaagcgag cactgtacgg aacgtgtcga tggtaacgct gaccgtctcc
      961 ggccacgacc acatccagtt catatcgatg tgcccatgcc cgacgttcca aaccgtgtag
     1021 tctttcgcca ccgcaccgat tggttcgagt atctgttccg ccttctcaac cgccgcgggt
     1081 aggtctgcac aggcgtttcc agaaaacgcg gcatttacct gatcgagggc gcgtccaatg
     1141 agctgttccc attcggtagc tttgtccgga tgcaattcgc gcagtcgcca cgcgaacgag
     1201 agttcggaag agattcgttc aattcgttct ttcacgcttc catgcgcggt atccagatca
     1261 cgcgatagcg ctgcaatctt tgcggtgatg tcgggcatgc atgtctcctt cgagcgcggt
     1321 tcggaccaca gtggaatcaa catcgattag catcgtgaac atagtaggta cgcaacgacg
     1381 atgtgggcgg gaagtgtcct cgtatatcat tcgcgtcaat ctcggtacaa cgacgaggtg
     1441 accgctttcg cgatcacctc gttcgcatcg ttgcctcgcc gcgcaaacgc ggcaaatcgt
     1501 ctattccgta ctcggcggca cggttggacg caaacgaatg cccagttctc ggagctgtcg
     1561 ttcatcgacc tcagaaggcg cac
//
LOCUS       DSCC01000135            1506 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1056489, whole genome shotgun sequence.
ACCESSION   DSCC01000135 DSCC01000000
VERSION     DSCC01000135.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1056489"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            236..1411
                     /locus_tag="ENN56_01580"
     CDS             236..1411
                     /locus_tag="ENN56_01580"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74209.1"
                     /translation="MRKHSRFALTSPSAARRRRVTPDTSACTLISYIAPAAPATRRWA
                     RGNEPFLRPEIGFTPKWFRENVPFEDGPIDFGEKWHTNPEYRRKTLLAARAELRRRFP
                     GTAIGHIDEPDQPLDVLTGTYGGSLIAAIFGVPIIWARDNWPNAAHQYLSDDEMAAIE
                     PADLDASPVFQDLMRQVDWIAESEGIITGFINPQGILNNAQRLRGETIFMDMFDNPDG
                     CRRLFDVIAETMIAVVLRVHARQRETGFDPQFFTVSNCLVNMVSPRQYHELLLPYDRR
                     IAEIYPCIGIHNCAWNANGYMDDYATVPNLGYIDMGIDSDLRRARELVPQGRRALMYT
                     PMDVANKDSVELRADFEKIADEYGPCDIVLADIEAGTPDARVRELVDICAEISAARA"
BASE COUNT          314 a          500 c          418 g          274 t
ORIGIN      
        1 caacggcacc gcgacctcga cgcaccgcaa cggacacacc gggtgaagac attcgtctcc
       61 aagcctcgca cggcgaaacg acggtgtcgc ttcgcctaaa cgaactcgaa cgcacgtgag
      121 agttcgcggg gatactgtca cccgcctctt cgctgtatct tcgcctgtac aatgccgacc
      181 ttcgacacca accgtcggac acctttcgcc cgaagatgcg gggcgaagcg aacgaatgcg
      241 taagcattcg cgcttcgcct tgacgtctcc gagcgcagcg aggagacgcc gtgtgactcc
      301 cgacacttcc gcttgcaccc tcatcagcta cattgcaccc gcggcaccag caacccggcg
      361 ctgggcgcgc ggcaatgaac cattccttcg accggagatc ggtttcacgc cgaagtggtt
      421 tcgcgaaaac gtcccattcg aggacggccc aatcgatttc ggcgagaagt ggcacacgaa
      481 tccggagtat cgccgcaaaa cgctactcgc agcgcgcgcg gaactccgtc gtcgcttccc
      541 cgggacggca atcggacaca tcgacgaacc cgatcaaccg ctcgacgtcc tcaccggcac
      601 gtacggcggc tcgctcatcg ccgcgatatt cggcgtaccg atcatctggg cgcgcgacaa
      661 ctggccgaac gccgcgcatc aatacctgag cgacgacgaa atggcggcca tcgaacccgc
      721 cgacctcgac gcgagtcccg tgtttcagga tctcatgcgg caggtcgatt ggatcgccga
      781 atccgaaggc atcataaccg gcttcatcaa cccgcaggga atcctcaaca acgcgcaacg
      841 gcttcgcggc gaaacaattt tcatggacat gttcgacaat cccgatggct gccgccggtt
      901 gttcgacgta attgccgaga cgatgatcgc cgttgtcctg cgcgtgcacg cccgtcaacg
      961 cgagaccggt ttcgatccgc aattcttcac cgtgtcgaac tgcctcgtca acatggtatc
     1021 gccacggcaa taccacgaat tgctcctgcc atacgaccgc cgaatcgcgg aaatataccc
     1081 gtgtatcggc atccacaact gtgcgtggaa tgcaaacgga tacatggacg actacgcgac
     1141 cgtgccgaac ctcgggtaca tcgatatggg gatcgattcg gatctgcgac gtgcgcggga
     1201 gctcgtcccg caaggccgtc gtgcgctcat gtacacgccg atggacgtcg cgaacaagga
     1261 ttccgtggag cttcgcgccg acttcgagaa aatcgccgac gagtatggtc cgtgcgatat
     1321 cgttctcgcc gacatcgaag ccgggacgcc ggatgcgcgc gtgcgcgagc ttgtggatat
     1381 ctgcgcggaa atcagcgccg cgcgggcgta accgacttcg cttgacctcg cgattcgcct
     1441 cccgtacttt tttctacgtt cgacgacacg gcccccgcat caaccggggg ccactttctt
     1501 ttgggc
//
LOCUS       DSCC01000136            2388 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030261, whole genome shotgun sequence.
ACCESSION   DSCC01000136 DSCC01000000
VERSION     DSCC01000136.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030261"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(14..610)
                     /locus_tag="ENN56_01585"
     CDS             complement(14..610)
                     /locus_tag="ENN56_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDS74210.1"
                     /translation="MARNSGRRDARNSVLDDVELSETVVKIKPVSKVTKGGRDRRFNA
                     LVVVGDGKGRVGVGMGKAREVAEAIRKGTQIAQRNIKRVPVGSTIPHEVWGQCGAARV
                     FLRPASPGTGVIAGAAVRSVIEAAGIKNVLTKSVGSTNAHNVAIATVDALAELRTYRE
                     VLATRRIEDEPRPARAAEVPVSEPVAQDAPVAEEGAES"
     gene            complement(632..979)
                     /locus_tag="ENN56_01590"
     CDS             complement(632..979)
                     /locus_tag="ENN56_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDS74211.1"
                     /translation="MKSRRELRLQRWRRARRVRGTAERPRLVVDRGLRSIQAHFVNDD
                     DSAVICGASTLAKSLEIHGSNIEAAKALGKELARRAKEKGITKVVFDRNGYPYHGRVK
                     ALADGAREGGLEF"
     gene            complement(1059..1628)
                     /locus_tag="ENN56_01595"
     CDS             complement(1059..1628)
                     /locus_tag="ENN56_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDS74212.1"
                     /translation="MSRIGRKPVPLPSGVTVDIDGRVVSAKGPKGNVAVTLPENVTVE
                     NDGGELKVAPEEGAIPGAWGLSRTLVSNAVTGVTDGFKRRLLIVGVGYRAEAQGSKLT
                     LTVGYSHPVVIEAPSTISFSVEPNITVSFKGSNHPTVPVVVEGADKQLVGDYAAKVRA
                     VRPPEPYLGKGIRYEEERIRTDKTGKAAR"
     gene            complement(1642..2040)
                     /locus_tag="ENN56_01600"
     CDS             complement(1642..2040)
                     /locus_tag="ENN56_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDS74213.1"
                     /translation="MSMTDPIADMLTRVRNACQAGHRTVDVPASNIKREIARILLEQG
                     YLNRVYVVDDKKQGMLRLYLRYDRDGNPIIRGLRRASTPGRRRYRGAQLVDRVLNGLG
                     IAIVTTSKGVMTDEECRKVGVGGEVLCHVW"
     gene            complement(2069..2254)
                     /locus_tag="ENN56_01605"
     CDS             complement(2069..2254)
                     /locus_tag="ENN56_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_431272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDS74214.1"
                     /translation="MAKKCLRVKATRKPKFKVRAYNRCPRCGRPRAFLRKYGMCRICF
                     RELALKGEIPGVVKASW"
     gene            complement(2274..>2388)
                     /locus_tag="ENN56_01610"
     CDS             complement(2274..>2388)
                     /locus_tag="ENN56_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDS74215.1"
                     /translation="IRGMDITFVTTAETDEEALAFLSLLGVPFVREASEAA"
BASE COUNT          423 a          791 c          650 g          524 t
ORIGIN      
        1 accgcgatct tcttcacgac tcagctccct cttcggcgac gggcgcatcc tgagctaccg
       61 gttcgctaac gggaacctcc gcagcacgtg cgggccgcgg ttcatcttcg atgcgacgcg
      121 tcgcgagcac ctcgcgatac gtgcgcaact ctgcgagcgc gtcgaccgta gcgatggcga
      181 cgttgtgggc gttcgtcgaa ccgaccgact tcgtcagcac gttcttgatg cccgccgctt
      241 caattacact gcgaactgcg gcgccggcga tcacaccggt accggggctg gccggacgca
      301 agaacacgcg cgctgcgcca cactgtcccc atacctcgtg cggaatcgtg ctgccgaccg
      361 gaacgcgctt gatattccgc tgggcgatct gcgtgccctt ccgaatcgct tcggcgactt
      421 cacgcgcctt gcccataccg acaccgacgc gtcccttgcc gtcgcctacc acgacgagag
      481 cgttgaaccg tcgatcccgt ccgccttttg taaccttgga gacgggcttg atcttgacca
      541 ccgtctcgct cagttcgacg tcgtcgagta ccgaattcct tgcgtctctt ctaccactat
      601 ttcgcgccat gctgatccct cttggggcga ctcagaattc gagtccgcct tcacgtgcgc
      661 catcggcgag agcctttacg cgcccatggt acgggtaccc gttccggtcg aatacgactt
      721 tcgttattcc cttttccttt gcgcggcggg caagttcctt gccgagcgct ttcgcggcct
      781 cgatgttcga accgtggatt tcgaggctct ttgccaacgt gcttgcaccg cagataaccg
      841 cactatcgtc gtcgttgacg aagtgggctt gtatgctcct gagtccgcga tcgacgacga
      901 gtcgtgggcg ttccgctgtc ccacgtactc gtctggcgcg ccgccagcgt tgcagccgca
      961 attctcgcct gcttttcatg atcgttcagt cctcggcgcg caccgagccg tgttcggatt
     1021 ccgggtacac ggagtgcgcg cggtatcggc ccgcgagatt agcgcgcggc cttgcccgtc
     1081 ttatctgtgc ggatccgctc ttcttcgtaa cggatgcctt tacccaggta cggctccggc
     1141 ggacgaaccg cgcggacttt cgctgcgtag tcgccgacaa gctgcttgtc cgcgccttcc
     1201 acaacaaccg gtaccgtcgg gtgattacta cctttaaagc tgacggtaat gttcggctcg
     1261 acgctgaatg agatcgtcga cggcgcctca ataaccaccg ggtgcgaata gccgacggtg
     1321 agcgtcaact tgctgccctg cgcttcggcg cggtaaccga ctccgacgat gagcagccgt
     1381 cgcttgaaac cgtccgtcac accggtgacc gcatttgaga cgagcgtccg cgaaagaccc
     1441 catgcgccgg gaatcgcacc ttcttcaggg gcgaccttca gttctccgcc gtcattctcg
     1501 accgtgacat tttccggaag cgtcacggct acgttgcctt tcgggccttt cgcactgacg
     1561 acacgtccgt caatatcgac cgttacccca ctcggcaggg gaacgggctt tcgtccaata
     1621 cgcgacatat gccgtttcct cctaccagac gtggcagagc acttcgccac cgacaccgac
     1681 cttacggcac tcttcgtcgg tcatcacgcc cttcgaggtc gtgacgatag cgatgcccag
     1741 cccattgagt acacgatcta ccaattgtgc gccgcgatac cgacgccgac ccggagtcga
     1801 cgctcggcgt aaaccgcgaa tgatgggatt gccgtcgcga tcataccgca agtacagccg
     1861 aagcatgccc tgctttttat catcgacgac gtatacccgg ttgaggtatc cctgctccaa
     1921 cagaatacgt gcgatctcgc gcttgatatt cgacgcggga acgtctaccg tgcggtgacc
     1981 cgcctgacac gcattccgga cgcgggtcaa catatccgcg atcgggtcag tcattgacat
     2041 gtgcgtttcc ttttcgatcc agtgcgggtt accaactggc cttcacaacg ccgggaatct
     2101 cgcccttgag cgcaagctcc cggaaacaga tgcgacacat tccatacttg cggagaaacg
     2161 cccgagggcg accgcaacga gggcatctgt tgtaggcgcg aactttgaac ttcggcttac
     2221 gtgttgcctt gacccgcaaa cactttttgg ccacgtgtcg tgtcctttcc tgatcaggca
     2281 gcttcgctcg cttcgcgcac gaacggaact ccaagcaacg agagaaacgc aagcgcctcc
     2341 tcgtcggtct ccgctgtcgt gacgaaggta atgtccattc ctcggatc
//
LOCUS       DSCC01000137            1975 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039139, whole genome shotgun sequence.
ACCESSION   DSCC01000137 DSCC01000000
VERSION     DSCC01000137.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039139"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            173..430
                     /locus_tag="ENN56_01615"
     CDS             173..430
                     /locus_tag="ENN56_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74216.1"
                     /translation="MHIDLDRGHTFASVYSALTDPVWGPWWKTLDLTMLTLGLWHGLT
                     GVWGIIRDYAMPAILQLILLGLLIVAGLAFGIMGYTTILSF"
     gene            486..>1975
                     /locus_tag="ENN56_01620"
     CDS             486..>1975
                     /locus_tag="ENN56_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007470272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDS74217.1"
                     /translation="MVAHVHKFDVVIVGAGGAGLRAALEVPREYSCAVLTKVFASRSH
                     TGTAQGGVCAALANEEEDKPIWHVYDTVKGSDYLGDQDAIEIMCEDAPRAIIELEHMG
                     LPFSRTPTGHIAQRRFGGHTRDFGESPVRRACYSADRTGLVMQHTLFENCIKHNVNFF
                     HEYHLTDLIVRDGKAVGLVAINMMDQELHIFHAKAILIATGGFGRAYRITSNAHAGTG
                     DGMWLAWQAGIPLEDMEFVQFHPTGLWKLGILVSEAARGEGGILRNKDGEAFMERYAP
                     SVKDLAPRDMVSRSIVMEIRAGRGIHAPDGTQYVHLDLTHLGEDVINHKLPEITGFAR
                     TYLGVEPLREPIPVTPTCHYAMGGIPTNADAEVIANADGDVIEGLYAAGEAACVSVHG
                     ANRLGTNSLLDIIVFGRRGGKAISKYLEKDANFAPIPADVDGRTRERVEQLTNGNGDE
                     SVPKLRAELQQLMTEKVGEFRNAADLTIAIEKIAELKQRTLRTKVS"
BASE COUNT          455 a          496 c          578 g          446 t
ORIGIN      
        1 gctattcctc gcggtatagt ggctgatgct cgcgcacgag ttactcggct tgctggagac
       61 ggctctgtca ttgcaccatc atacatcgag caacacagga tcgttttgat ggttattcag
      121 gcgcatcagc gggttcgtcc tggtcgcgct tgtcattttc cactacgtac ttatgcacat
      181 tgatctcgat cgcggtcaca cgtttgcatc cgtgtactcg gcgttgacag atccggtatg
      241 gggtccgtgg tggaaaacac tcgatctaac aatgctcaca ctcggtcttt ggcacggact
      301 taccggcgtc tggggcataa ttcgcgacta tgcgatgccg gcaatcctgc aactgatact
      361 tttgggactc ctgattgttg cgggattggc atttggtatc atggggtata caacaattct
      421 gtcgttttga aacactcacg caccgacata cgtagcattc aggtgtggat agaggaagga
      481 agccgatcgt ggcgcacgtt cataaattcg acgtggtcat agtgggagcg ggtggagccg
      541 gtcttcgtgc cgcgcttgag gtgcctcgcg aatattcctg cgcggtattg acgaaagtgt
      601 tcgcatcacg ttctcatacc ggtacggcgc aaggtggtgt atgtgccgct ctcgctaacg
      661 aggaagaaga caagcccatt tggcacgtgt atgatacggt aaaaggatcg gactatctcg
      721 gcgaccagga tgcaatcgag atcatgtgtg aagatgctcc gcgagcgatt atcgaactcg
      781 agcatatggg gctcccgttt tcccgtactc cgaccggaca catcgctcag cggcgtttcg
      841 gcggtcatac gcgcgatttc ggagaatctc ccgtccggcg tgcatgctac tccgcggacc
      901 gtaccggtct cgtgatgcag catacgttgt tcgagaattg tatcaagcac aatgtcaact
      961 tcttccacga gtatcacctg accgatctca tcgtacgcga cggaaaagct gtcggtctcg
     1021 tcgcgatcaa catgatggat caagaacttc acatcttcca cgcaaaggcg atactgatcg
     1081 cgaccggagg attcggacgc gcataccgga tcacatcgaa cgcacatgcc ggtaccggtg
     1141 atggaatgtg gctggcatgg caggcgggaa taccgctcga agatatggag ttcgtacagt
     1201 tccatccgac ggggttgtgg aaactcggaa ttctcgtatc ggaagcagcg cgcggcgaag
     1261 gcggcattct tcggaacaaa gacggcgaag cgtttatgga gagatatgct ccgagcgtaa
     1321 aagacctcgc accgcgcgac atggtatctc gttcgatcgt catggagatt cgggctggtc
     1381 gtgggataca cgctcctgac ggcacgcaat acgtgcatct cgatctcaca cacctcggtg
     1441 aggatgtaat caatcacaag ctgccggaga ttacgggttt cgcaaggacg tatcttggtg
     1501 tggagccact tcgcgagccg attccggtca caccgacgtg ccattatgcg atgggtggga
     1561 taccaactaa tgctgacgca gaagtgattg ccaacgcgga cggcgacgtt attgaggggc
     1621 tctacgccgc aggtgaagcg gcgtgtgtat cagtacacgg cgcgaatcga cttggtacga
     1681 atagcttact cgatatcatc gtgtttggtc gtcgaggtgg taaagccata tcgaaatacc
     1741 tcgagaaaga cgccaacttc gcacctattc cggcagatgt cgacggacgg acgcgagagc
     1801 gtgtcgagca gttgacgaac ggtaacggag acgaatcggt accgaagctt agagcggagc
     1861 ttcagcaatt gatgacggag aaggtaggtg agtttcgcaa cgcagcggac ctgacgatcg
     1921 cgatagaaaa aatcgccgag ctgaagcagc ggactctgcg aacgaaggtt tccga
//
LOCUS       DSCC01000138            1738 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046563, whole genome shotgun sequence.
ACCESSION   DSCC01000138 DSCC01000000
VERSION     DSCC01000138.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046563"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1738)
                     /locus_tag="ENN56_01625"
     CDS             complement(<1..>1738)
                     /locus_tag="ENN56_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDS74218.1"
                     /translation="IGAKHAIAEANRPVFLQTQVTLIDASGRMLLGTDIGSAMTTINA
                     IGTDLVGINCSTGPMEMTDAVRYLGEMSRAPISVIPNAGMPINEDGETVYPMGPDEMA
                     EALARFVRDFGVSVVGGCCGTTPDHIRRFVKAVGIPTPAKQRSVPDTIYVSSSMTSVA
                     LEQEPRPLLIGERVNTVGSRKVKELLLTNQYEGLVPIARDQVEGGAHVLDVCTALTER
                     DDEIDQMRAVVKLLSQSVPAPLVIDSTEADVIEDALTWLPGVGIVNSINLEGDGSRVR
                     TVLPIVKKFGAAVIALTIDEEGMAHTADRKFAIAKRIYDTATNDYGIEPGRLIFDCLT
                     FPLTTGQEDLRNSAVETIEAIHRIKSDLPHALTVLGVSNVSFGVKPHARGVLNSVFLH
                     HCVQAGLDAAIVNPKHITPYASIPEDERAVTDDLVYNRHKDALATFIQHFESVVADTQ
                     HREDPRLHMTPARIIHYNIVNRDPEGIEELIDRELEACSAVEVINDVLLPAMQEVGDK
                     FGSGELILPFVLESAGVMKRAVAHTEQFLEKNDNTNKGDVVLATVYGDVHDIGKNLVR
                     TIFSNNGYTVHDL"
BASE COUNT          337 a          474 c          502 g          425 t
ORIGIN      
        1 gaggtcgtgc acggtgtatc cgttgttact gaagatggtg cgtacgaggt tttttccgat
       61 gtcgtggaca tctccgtaca cagtcgcgag aactacgtcg cctttgttcg tgttatcgtt
      121 cttttcgaga aactgttcag tatgagcgac agcccgtttc atgaccccgg cggattcaag
      181 cacaaacggt agaatgagct cacccgaccc gaatttgtcg ccgacttctt gcatcgccgg
      241 gagaagaaca tcgttgatta cctccactgc gctgcacgcc tcgagttcgc gatcgatgag
      301 ttcttcgata ccctccgggt cgcgatttac gatgttgtag tgaattattc gcgcgggtgt
      361 catatgcagg cgtggatctt cgcgatgttg tgtgtccgcg actaccgatt cgaaatgctg
      421 gatgaatgtt gcgagggcgt ccttgtgccg gttgtacacg aggtcatcgg taacggctcg
      481 ttcgtcttca gggatggacg cgtaaggcgt gatgtgtttc ggattcacga tcgcggcgtc
      541 gaggcctgct tgcacgcagt gatggagaaa cacgctgttg aggacgccgc gcgcgtgtgg
      601 ctttacgccg aatgacacat tactcacgcc gagcacggtg agagcgtgcg gaaggtccga
      661 cttgatgcga tgaatcgcct cgatcgtttc gacagcgctg ttccgaagat cctcttgtcc
      721 cgttgtgagt gggaacgtaa ggcaatcgaa gatcaggcgt cccggttcga tcccgtagtc
      781 attcgtcgcg gtgtcataga tgcgtttcgc gattgcgaat ttccgatccg cggtgtgcgc
      841 catcccttct tcgtctatcg tgagtgcgat aaccgcggca ccgaactttt ttacaatcgg
      901 aagaacggtg cgaactcgtg agccgtcgcc ttcgagattt atcgagttga cgataccgac
      961 tccgggcagc cacgtaagcg catcttcgat gacatcggct tcggtgctgt caatcacaag
     1021 tggtgctggt acagattgcg aaaggagctt gacgactgcg cgcatctgat cgatctcgtc
     1081 gtcacgctcg gtaagcgccg tacaaacatc gagtacatgc gcaccgcctt ccacttgatc
     1141 tcgcgctatt ggcacaagcc cttcgtactg gttggtaagg agtagttcct tcaccttgcg
     1201 actaccgacc gtattaacgc gctcgccgat gagtaacggg cgcggctcct gctccagcgc
     1261 gacgcttgtc atcgacgatg acacataaat ggtatccggg acgcttctct gtttggctgg
     1321 tgtgggaata cctactgctt tcacaaaccg acgaatgtgg tccggcgtcg ttccgcaaca
     1381 gcctcctacg acgctgacgc cgaaatcgcg gacgaaacgt gcaagcgctt cagccatctc
     1441 atcgggaccc attgggtaga ccgtctcgcc atcctcgttg atgggcatac cggcgttcgg
     1501 aatgacgctg attggtgcgc ggctcatttc gccgaggtat cgcacagcgt cggtcatttc
     1561 cattgggccg gtagaacagt tgatgccgac gagatcggtg ccgattgcgt tgatcgtcgt
     1621 catcgcgctc ccgatatcgg taccgagaag catgcgccca ctcgcatcga tgagcgtgac
     1681 ctgtgtctgg agaaacaccg gtcgattcgc ctcggcgatc gcgtgcttgg cgccaatc
//
LOCUS       DSCC01000139            4648 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1012395, whole genome shotgun sequence.
ACCESSION   DSCC01000139 DSCC01000000
VERSION     DSCC01000139.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1012395"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..378
                     /locus_tag="ENN56_01630"
     CDS             <1..378
                     /locus_tag="ENN56_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDS74219.1"
                     /translation="IPRELREGSLALGATKWQTIVRNVLPYSIPGMLTGAILGIGRAA
                     GETAPILLTVAAFYLPTLPNSVFDQAMALPYHLYVLATQHPDIEKVRPMQYGTALVLL
                     TIVLGISMVAIVLRSRFRKKIRW"
     gene            372..1160
                     /locus_tag="ENN56_01635"
     CDS             372..1160
                     /locus_tag="ENN56_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDS74220.1"
                     /translation="MVEQTVTTASKPNIKIRAESVDFYYGSVQALFGISMDINEHEVT
                     ALIGPSGCGKSTFLRTLNRMNDIIDDTRTEGKIRIDGHDIYAPETDVVELRKRVGMVF
                     QKSNPFPKSIYDNVAYGPRIHGQKNRSELDDIVEQALRGAALWDEVKDRLSISALDLS
                     GGQQQRLCIARALAVDPDILLMDEPASALDPRSTSRIEDLIGELRENYTIVIVTHNMQ
                     QAARVSDYTAFFYEGTLVEFGPTQQVFTKPDKQQTEDYITGRFG"
     gene            1180..1851
                     /gene="phoU"
                     /locus_tag="ENN56_01640"
     CDS             1180..1851
                     /gene="phoU"
                     /locus_tag="ENN56_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDS74221.1"
                     /translation="MTMHLQREIEGLKKMLLSLSAYVEESVQKAVRALADRDSALAQR
                     VIDDDTIIDKMEVDVEEECLKVLALHQPVAIDLRFIISVLKINSHLERIGDLSVNIAE
                     RALYLNEVDPIPGPFDFLGMATKAQSMLRRSLDALITMDSRLAREVCADDDEVDQINR
                     KMHKKVKEEIKKDLDRFDCYIHLLGISRHIERIADQATNIAEEVVYMTEGEIVRHRFE
                     DFRAE"
     gene            2090..2731
                     /locus_tag="ENN56_01645"
     CDS             2090..2731
                     /locus_tag="ENN56_01645"
                     /inference="COORDINATES: protein motif:HMM:PF00154.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HDS74222.1"
                     /translation="MISEHTAIARTLSRLPDIVSAQSLMHDTDARTWRYDALCGRLIE
                     LSSADAAASLTATTTLIAQAQREGEPVAWVTTEESCVYPPDLSEQGIDLDALIVVRIG
                     TTTDVPRAAEKLVQSGAFGLIVRDLVERAHIPTPLLARLMQLARKHATVVVCLTVKPS
                     DEESLSSLVSLRGEARRIRREGVIYRFEVHALKNKRGSPWRHTEQCHGPMGLR"
     gene            2709..4166
                     /locus_tag="ENN56_01650"
     CDS             2709..4166
                     /locus_tag="ENN56_01650"
                     /inference="COORDINATES: protein motif:HMM:PF00817.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HDS74223.1"
                     /translation="MDRWACVNIPALPLQFALVQRPDWKAYPVVIISRDAPNGEVMWL
                     NKKAARSGIRKGLRYATALEIEPDLRANVISRTTISRQIKRIENTLQTVTPDVESSDD
                     IPGVFWLNATGMGGVYGTISRWGKRIIAILKECGYQATVTIGFTRFGTYAASAAHTGV
                     TIFRDRAEELQTVHEIKLRDLSLPSDLVDTLGMLGIETVDGFIALPADGLRKRFGETA
                     YTLHRWASGDMWDPLRPERREPTLYSRIEFERPESDALRLFTAISSVLQPLIHQTSAR
                     GNAVREVRWVMLLDTGERRSESVKPATPSIDERQIGDLVRLRIESLQLDAGVDELLIS
                     VQTVPTQAGQTSLLTVRQKRDLAAADRALARLRAEFGDNAVVRACLEDRQLPEARYRW
                     EPVDRARFPKPVQTSLLSLVRRVYTKPRPIQQACMVTKIAGPYVVSGGWWYTTPESKE
                     EVHREYYFVLTTNEAILWVYYDRANQQWFAQGEVA"
     gene            4170..>4648
                     /locus_tag="ENN56_01655"
     CDS             4170..>4648
                     /locus_tag="ENN56_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDS74224.1"
                     /translation="MYAPLWCKSHYSFCEGASSPSELVEQTAHFGMKHVAITDRDGVY
                     GVVRAHVAARDYGIHLIVGSEITLDDGSTILLLVMDRKGYTNLCRLITVGRRRCEKGS
                     SHVTWQEICAHSEGLIALWGGERSLISCHDEIDAATVGGLREAFGDRLYGLVARHCR"
BASE COUNT         1119 a         1204 c         1273 g         1052 t
ORIGIN      
        1 atccctcgcg agcttcgcga aggatcgctt gcgcttggtg cgacgaagtg gcaaacgatc
       61 gtacggaatg tcttgccgta ctcgataccg gggatgctta ccggtgccat cctcggtatc
      121 ggacgtgccg ccggcgaaac ggcgcccatt cttcttaccg tcgcagcgtt ctatctaccg
      181 actctaccaa actcggtatt cgaccaggcg atggcgttac cgtatcacct ttacgttctt
      241 gcgacacaac acccggacat agaaaaagtt cgtccgatgc agtatggcac agcgttagta
      301 ctcctgacaa ttgtattggg gattagtatg gtagctattg tcttgcgtag tcgattcaga
      361 aagaaaatca gatggtagag cagaccgtaa caaccgcatc gaaaccgaac atcaagattc
      421 gagccgagtc ggttgatttt tactacggat cggtacaggc gttgttcgga atatcaatgg
      481 atatcaacga acacgaagta acggcgctga tcggaccgtc gggatgcggg aaatcgacat
      541 ttcttcgcac attgaaccga atgaatgata tcatcgacga cacacgcacc gaggggaaga
      601 tccgcattga tgggcacgat atctatgcac cggagaccga tgtcgtagag ctgcgtaagc
      661 gcgtggggat ggtgtttcag aaatccaatc cgtttccgaa atcgatctac gataacgtcg
      721 cgtatggccc gcgtatacac gggcagaaga accgatcgga actcgacgat atcgtcgagc
      781 aggctttacg aggtgctgcg ctgtgggacg aggtgaaaga tcgcttgtca ataagtgcgc
      841 tcgatctctc gggcggccaa cagcaacgtc tctgtattgc tcgggcactc gcggtcgatc
      901 cggacatatt gctcatggac gaaccggctt cagcactcga tccacgatcg acgagtcgca
      961 tcgaagatct cattggcgag ttgcgggaaa actacacgat cgtcatcgtt acgcacaaca
     1021 tgcagcaggc cgcccgcgta tccgattata cagcgttttt ctatgaagga acgctcgtgg
     1081 aatttggccc cacgcaacag gtgttcacga agccggacaa acaacagacc gaagattaca
     1141 tcaccggtcg ttttggttga tgggggatac tgatttatta tgacgatgca tttacaacgc
     1201 gaaatcgaag gcctgaaaaa gatgcttctt tcgctgagcg cgtatgttga agagagcgtt
     1261 cagaaagctg tacgtgcact cgcggataga gactcagcgc tcgcgcagag agtcatcgat
     1321 gacgacacga ttatcgacaa aatggaagtc gatgtcgaag aggagtgtct caaagtactc
     1381 gcgctccatc agccggtggc aattgatctc cgctttatca tttccgtgct caagatcaat
     1441 agccacttgg agcgaatcgg tgacttatcg gtaaatatcg cggaacgtgc gctctacttg
     1501 aacgaagtgg atccgattcc cggaccgttc gatttcttag gcatggcgac gaaagcacaa
     1561 tcgatgcttc ggcggagtct ggatgcgctc atcacgatgg atagcagact cgctcgagag
     1621 gtctgcgccg acgacgatga agttgatcag atcaatcgaa agatgcacaa aaaagtcaaa
     1681 gaagagatca agaaagacct tgatcgtttc gactgttaca tccacctact cggaatatcg
     1741 cggcatatcg agcgaatcgc cgaccaagcg acgaacatcg ctgaggaagt cgtatatatg
     1801 acagaaggcg agatcgtccg gcatcgtttc gaggatttcc gggcggagtg aaagagctct
     1861 gagtttgatt cgcctctctc cgcgcctaac gcccttcaag aggctgttag gcgcggtttt
     1921 cccatcgtgt atgccgttcc cccgcctttc aattcattcg ctgtcgcaat ccactctccg
     1981 gaccctattc gtccatacac tccttcatct tgccataccc gcataatacc cgtattatta
     2041 cgggtatact tcgccctgaa taacgtcgca taaacctatc ggcactgata tgatctctga
     2101 gcacacggcg attgcgcgca cattatcccg attgccggac atcgtatccg cacaatcact
     2161 gatgcacgat acggatgccc gtacgtggcg ctatgatgca ctctgcggtc ggctcatcga
     2221 actatcgagc gctgatgccg ccgcgtctct tacggcaaca acgactttga ttgcgcaggc
     2281 gcaacgagaa ggagaaccgg ttgcatgggt aaccacggag gaaagttgcg tctatcctcc
     2341 ggatctttcc gagcagggta ttgatctgga tgcgctgata gtcgttcgaa tcggcacaac
     2401 aacggatgtg ccccgtgcag cggagaaact tgtgcaatcc ggtgcattcg gacttatcgt
     2461 gcgcgatctc gttgaacgcg cgcacattcc gacgccgcta ctcgcacgat tgatgcaact
     2521 cgcgcgaaag cacgccacag tggtagtatg tctcacggta aagccttcag acgaagagtc
     2581 actgagctct ctcgtctctc ttcgcggaga agcccggcgg atacgaaggg aaggagtgat
     2641 atatcgattc gaggtacatg ctctaaaaaa caaacgtgga tctccatgga gacatacgga
     2701 acagtgtcat ggaccgatgg gcttgcgtta acattccggc attgccgctc caattcgcac
     2761 ttgtgcagcg ccccgactgg aaagcatatc ccgtggttat catctctcgc gatgcgccga
     2821 acggagaggt catgtggctc aacaaaaaag ccgcccgatc gggcattcgg aaagggctac
     2881 gatacgcaac tgcattggag atcgaacccg atcttcgcgc aaacgtgatt tcccggacga
     2941 caatatcgcg acagataaag agaatcgaaa acactttaca aacagtgact ccggatgttg
     3001 agtcttcgga cgatattccc ggagtgtttt ggctgaacgc gaccggcatg gggggtgtct
     3061 atggcacaat ttcccgatgg ggtaagcgga tcatagcgat actgaaggaa tgcggatacc
     3121 aagcgaccgt gactattgga tttacccgat tcggaacata tgcagcctcg gcggcccata
     3181 cgggagtaac gatcttccgc gaccgagccg aagaacttca aacggtgcac gagatcaagc
     3241 ttcgcgatct ctccctcccg tccgacctcg ttgatacact cggtatgctt ggtatcgaga
     3301 ctgtcgacgg gtttattgca ctccctgcag atggtcttcg aaagcgattc ggggagacgg
     3361 cgtataccct ccatcgttgg gcttccggcg atatgtggga tccgcttcgg ccggagcgac
     3421 gtgaacccac actgtatagc cgtattgaat tcgaaaggcc ggaaagcgat gctttgagat
     3481 tatttacagc gatatcctcg gttcttcagc ctctcataca tcagacctcc gcacgtggga
     3541 atgcagtgcg tgaagttcgc tgggtgatgt tgcttgatac cggtgaacgc cgttcggagt
     3601 ctgttaaacc agcgactccg agtatcgatg agcgacagat cggcgatctt gtccgcctgc
     3661 gcatcgagtc gctacaactc gatgccggtg tcgatgagct tctcatcagt gtccagaccg
     3721 ttccgacaca agcggggcag acatcccttc tcacggtacg acagaaacga gaccttgccg
     3781 ctgctgaccg agcattggcc cggcttcgtg ccgaattcgg agacaacgcg gttgtccgtg
     3841 cctgtcttga ggatcgtcaa cttcccgaag cgcggtatcg gtgggaaccg gtcgaccggg
     3901 cgcggttccc aaaaccggta caaacatcgt tgctatcgct cgttcgacgt gtatatacca
     3961 agccgcgacc aatccagcag gcatgtatgg tgacgaaaat cgccggtcct tacgtcgtct
     4021 ccggcggatg gtggtatacc acccccgaat cgaaagaaga ggtgcatcga gagtattact
     4081 tcgtgcttac gacgaatgaa gcgattctgt gggtctatta tgaccgggca aatcagcagt
     4141 ggtttgcaca aggcgaggtt gcgtaagaga tgtacgcccc gctgtggtgc aagagtcact
     4201 attcgttctg cgaaggtgca tcgagcccga gtgagttggt agaacaaacc gcacacttcg
     4261 gcatgaaaca tgtggcgatc actgatcgcg acggtgtgta cggcgtcgtt cgagcgcatg
     4321 tggcggcgcg cgattacggc attcacctta ttgtcggttc agaaatcacc ctcgatgacg
     4381 gatcaacgat tctcctcttg gtaatggatc gaaagggata caccaatctg tgccgattga
     4441 tcactgtggg acggagacga tgcgagaaag gttcgtctca cgttacatgg caggagattt
     4501 gtgcgcattc agagggtctc attgcgcttt ggggaggcga gcggagcctc atcagttgtc
     4561 atgatgaaat cgatgctgcg acagtaggcg gcctccgtga agcgttcggt gatcgcctat
     4621 atggtcttgt tgctcgtcat tgcagaga
//
LOCUS       DSCC01000140            2347 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030979, whole genome shotgun sequence.
ACCESSION   DSCC01000140 DSCC01000000
VERSION     DSCC01000140.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030979"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            3..1046
                     /locus_tag="ENN56_01660"
     CDS             3..1046
                     /locus_tag="ENN56_01660"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74225.1"
                     /translation="MTWLHEHDHARAGKPWFLCASFSRPHFPLTAPRRFFDRYWPNGV
                     TPPKTGPGDTHDHPMTRGMRAGFHTDEIGSEEAMRARAAYFACVDYLDEILSDFLTLL
                     DADGFLENTIIVYTTDHGELAGEHGLWWKNSWHEAATRVPWFIQTPEHRSGAIAPRDV
                     DTPVSLADLFPTLAGLCGVETPSDIDGIDLSNSVIDSGEPAQRPVFCDALTPRWGAGT
                     EFRMVRDGTWKYVAFRNAPPILIDLESDPYEQHSLAGTADASVEAKDAERRLAKIVAE
                     SIDFDDVEAERLRDEQLTKEHALRIKPGYRNMYLLADGRLIDADREIYEPPVLSDDPA
                     TTFADFPGRDRSE"
     gene            1196..1771
                     /locus_tag="ENN56_01665"
     CDS             1196..1771
                     /locus_tag="ENN56_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDS74226.1"
                     /translation="MPELKGSRTEKNLMIAFAGESQARNRYSYFASQAKKDGFVQIAD
                     IFEETANHEKEHAKRFFKFLKGGDLEITASFPAGVIGTTLENLRAAAAGEKHEWTEMY
                     PEFAEIAREEGFGAIAKTFESVAVSEKQHEKRFVELAENVATGKVFKREGKVVWRCRN
                     CGYLHEGTEPPAVCVACAHPKDHFELLGENW"
     gene            complement(1844..>2347)
                     /locus_tag="ENN56_01670"
     CDS             complement(1844..>2347)
                     /locus_tag="ENN56_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS74227.1"
                     /translation="EYLKGFEKMELVSTGSLLGVRETRRIVGDYELNLDDFTSRAVFP
                     DEIGRYSYPVDIHIARPDKESYDNFRKEMATYRLGKGESYGIPYRILTPKGLKNVLVA
                     GRCVSTDRSLQASIRVMPGCYITGQAAGMAATLMVEGNTDSRGIAVNELQRRLVEMGG
                     YLPNYGG"
BASE COUNT          514 a          659 c          701 g          473 t
ORIGIN      
        1 ctattacatg gctccatgaa cacgaccatg cacgggcggg gaagccatgg tttctctgcg
       61 cgagtttcag caggccgcac tttccgctca ccgctcctcg gcggttcttc gatcgttact
      121 ggccgaacgg cgtgacgcca ccgaagacgg gaccgggcga cacgcacgat cacccgatga
      181 cacggggaat gcgagccggt ttccacaccg acgaaatcgg cagtgaggaa gcgatgcgtg
      241 cgcgcgccgc gtatttcgct tgcgtcgact atctcgatga gatactcagc gatttcctca
      301 cgcttctcga tgccgacgga tttctcgaaa acactatcat cgtgtatacg accgaccacg
      361 gcgagctggc cggtgaacac ggcctctggt ggaagaactc gtggcacgag gcggcgactc
      421 gggtaccgtg gttcattcaa acaccagagc atcgctcagg agccatcgca ccgcgcgatg
      481 tcgatacacc cgtgagtctt gccgatctct tcccgacgct cgccggactg tgtggcgtcg
      541 aaacgccgtc cgacatcgac gggatcgatc tttcgaattc ggtgatcgat tctggtgaac
      601 ctgcgcagag accggtattc tgcgatgcgt tgacgccgcg ttggggcgcc ggcacggagt
      661 tccgcatggt acgtgacggc acgtggaaat atgtggcgtt tcgaaatgca ccgccgattc
      721 tcatcgacct cgagagcgat ccatacgagc aacacagtct cgctggaaca gccgacgcgt
      781 ccgtcgaagc gaaggatgcg gaacggcgac tcgcgaagat cgttgccgag tcgatcgatt
      841 tcgacgatgt agaagcggag cgtctgcgcg acgaacagct aacgaaagag catgcgctcc
      901 gtatcaagcc aggttatcgg aacatgtacc tgctcgccga tggtcgtctt atcgatgcgg
      961 atcgtgagat ttacgagccg cccgtgctct cggatgatcc agcgacaaca ttcgcggatt
     1021 ttccgggccg agaccggtcc gaatagcagc gatttctatg caagtatcaa cggtggagtg
     1081 gggatttcga gatactttgt ccgaacgacg gctgtttgta cgttttatcg tgaattcgag
     1141 aacgacgacc ccacacgtcg cgtatgcaga tagccttgtc cgaaaggggt agcagatgcc
     1201 ggaactcaag ggatcacgca cagaaaagaa cctcatgatc gcgttcgccg gggaatcgca
     1261 ggcgcgaaat cggtactcgt atttcgcgag ccaggcaaag aaggacgggt tcgttcagat
     1321 tgccgatatc ttcgaagaga ccgcaaacca cgagaaagag catgcgaagc gtttcttcaa
     1381 gttcctcaaa ggtggcgatc ttgagatcac cgcaagtttc ccggcgggag tgattgggac
     1441 aacactcgag aatcttcgtg cggcagcggc tggtgagaaa cacgagtgga cggagatgta
     1501 cccggagttt gccgaaatcg ctcgcgaaga gggctttggc gcgatcgcga agacgttcga
     1561 aagcgtcgcc gtgtcggaaa agcagcacga gaagcggttc gttgagctcg cggaaaacgt
     1621 tgcgaccgga aaggtcttca agcgcgaagg gaaggtcgtg tggcgttgcc ggaattgcgg
     1681 ctacctgcat gagggtaccg aaccgcccgc cgtgtgcgtg gcgtgtgcgc acccgaagga
     1741 tcacttcgag ctgctcggcg agaactggta agaacaagaa cgggaggcgt tctcacgcct
     1801 cccgttttgc gcttccgcgc acgtcatcgg atacgccgaa cggctatccg ccgtaattcg
     1861 gcaggtaccc gcccatttca acgagccgcc gttggagttc attcacggct atcccacggg
     1921 agtccgtgtt cccctcaacc atcaaggtcg cagccatacc ggctgcctga ccggtgatgt
     1981 aacatcccgg catgacgcga atcgacgcct gaagtgaccg gtccgtgctc acgcatcgac
     2041 cggcgacgag gacgttcttc aggcctttcg gcgtgagtat gcggtagggg ataccgtagc
     2101 tttcgccctt tccaagccga tacgtcgcca tttccttgcg gaagttgtcg tagctttcct
     2161 tgtccggacg cgcgatatgg atatcgacag gatacgagta acgtccgatt tcgtcgggga
     2221 acaccgcgcg actggtgaag tcgtcgagat tcagctcata atcaccaacg atacgacggg
     2281 tttcgcgaac accgagcagt gaaccggtgc tcacaagctc catcttctcg aatcctttga
     2341 gatactc
//
LOCUS       DSCC01000141            1834 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043371, whole genome shotgun sequence.
ACCESSION   DSCC01000141 DSCC01000000
VERSION     DSCC01000141.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043371"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..289
                     /locus_tag="ENN56_01675"
     CDS             <1..289
                     /locus_tag="ENN56_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDS74228.1"
                     /translation="GLVTDLLERQLDSASTPSEVFACGPTPMLLAVARVARAHSVPCQ
                     VSLEEHMACGIGICMGCAVRKAGTPESDYSLVCVDGPVFDADDVFSTGIPQ"
     gene            286..1326
                     /locus_tag="ENN56_01680"
     CDS             286..1326
                     /locus_tag="ENN56_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74229.1"
                     /translation="MISPEQASRFLQGLSRFASSTGVLRLFEWTRRSGLTILRMRLSP
                     TSRDAAGDAWTAEMLTEALQLVAERYTVIPLRDAVAALEGRQAMPEAPLVLTASITTP
                     PGDFPLGRVLSAAKVQCFISGSPTLLDRGQPPWPVLVRTALDRVSTDSVDFYERRWPI
                     GGNGTREKVAKEIIGRLYALPDTERHSRAIELIGRWGVSADTVRGIQWTDFERLANTD
                     GVTVGASGLIGDSLLRQPADRAIWELRESRYRMSDRLGREIVYLEYPWGEMNPLLGIE
                     AKRAGYVCGIGWSTDSTAVLNPRDRDPFALISRPFYPGTREMTRAELAGLMTYLRPAV
                     RFITRKRRKVAQ"
     gene            1385..>1834
                     /locus_tag="ENN56_01685"
     CDS             1385..>1834
                     /locus_tag="ENN56_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74230.1"
                     /translation="MSVRYHLRYQIAPEHDVEKVTTELAAFCRKHEIEEVVLFYGAEM
                     FNSGLLSSADEDRWFDTIRRSTEILHTAGIDYSLNPWMTVLHTDRGRSMPADRSFAPM
                     VSPAGETATAVASFADPAWREYIAHQYGRFAGLGFRVVWVEDDYRYHN"
BASE COUNT          362 a          513 c          559 g          400 t
ORIGIN      
        1 gggtctcgtt accgaccttc tcgaacggca actggatagc gcgagtacac cttcggaggt
       61 gtttgcgtgc ggaccgacgc cgatgctttt ggcggttgca cgggtggcac gagcgcattc
      121 ggtaccgtgt caggtttcgc tggaagaaca tatggcgtgc ggtatcggca tttgtatggg
      181 atgcgcagta cgaaaggccg gtacgcctga gtcggactac tctctcgtgt gtgtcgacgg
      241 tccggttttt gatgctgatg acgtatttag cactggaatt cctcaatgat atcaccggaa
      301 caagcgtcga gattccttca agggctgagt cgctttgcgt cgagtaccgg cgttctgcgg
      361 ttgttcgagt ggacgcgtcg atcgggattg acaatcctgc gtatgcgcct ctcgccgaca
      421 tcgcgcgacg cggcgggcga cgcgtggaca gccgagatgc tcaccgaagc gctccaactc
      481 gttgcggaac ggtacaccgt aattcctctc cgtgatgccg tcgcggcact tgaaggacgc
      541 caagcgatgc cggaagcacc actcgtgctc accgcgtcga ttacgacgcc gccgggtgac
      601 ttccccctcg gacgtgtgtt atcggcggcg aaggtgcagt gttttatcag cggatcgccg
      661 acgcttctcg accgtggtca gcctccctgg ccggttctcg ttcggacagc acttgaccgc
      721 gtctcaacag actccgtcga tttctacgaa cgtcgttggc cgattggtgg gaacggcacc
      781 cgtgagaagg tggcgaagga gatcatcgga cgcctgtacg ccttaccgga cacggagcga
      841 cattctcgag cgatcgaact tattggtcga tggggagttt ccgcggatac agtacgtggt
      901 atacagtgga ccgacttcga acgactcgcg aatacggacg gtgtaacggt aggcgcgagt
      961 ggcctgatcg gtgattcgct tctccgccag ccggccgatc gtgcgatctg ggagcttcgc
     1021 gaatcgcggt acaggatgtc cgaccgactc gggcgggaga tcgtgtacct cgaatacccg
     1081 tggggcgaga tgaatccact cctcggtatt gaagcgaaac gtgcgggata cgtatgcgga
     1141 atcgggtggt ctaccgactc gacggcggta ttgaatccac gcgatcgtga tccgtttgca
     1201 ttgatcagca gaccgttcta cccgggtacg cgtgaaatga cacgtgcaga gctcgccggt
     1261 ctcatgacgt acctgcgtcc cgccgtccgg ttcatcacgc gaaaacggcg taaagtggca
     1321 caataattcg gtagcaacgc acctttccta cccacaactc cacgcatgca cgggagacgt
     1381 ttccatgtct gttcggtatc atctccgcta tcaaatcgca cccgagcacg atgtcgaaaa
     1441 agtgacgacg gaactcgcgg cgttctgtcg taaacatgaa atcgaagagg tcgtgttgtt
     1501 ttatggtgcc gagatgttca actccggact gctcagcagc gccgacgaag atcgttggtt
     1561 cgatacgatt cgccgcagta cggaaattct ccacacagcg ggaatcgact actcgttgaa
     1621 cccatggatg accgtgttgc acaccgatcg tggccgctcc atgcctgccg accggtcctt
     1681 tgcgccgatg gtatccccgg cgggagagac tgcgacggcg gtcgcgtcgt ttgccgaccc
     1741 ggcgtggcgc gaatatatcg cgcatcaata cggacgattc gcaggactcg gctttcgtgt
     1801 ggtctgggtg gaagacgatt atcgctacca caac
//
LOCUS       DSCC01000142            2211 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033583, whole genome shotgun sequence.
ACCESSION   DSCC01000142 DSCC01000000
VERSION     DSCC01000142.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033583"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="ENN56_01690"
     CDS             complement(<1..1072)
                     /locus_tag="ENN56_01690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDS74231.1"
                     /translation="MSTQNRNKPWRGVIEQYREYLPVTDSTPVITLLEGNTPLIPAVR
                     LPNEIGVDLELYLKFEGMNPTGSFKDRGMTLAISKAKESGAEIVVCASTGNTSASAAA
                     YAARSSMIPVVFIPRGNIAQGKLAQAMMHGALVMEIDGNFDDCLRLVREVADARPVAV
                     VNSINPFRIEGQKTGAFEICDQLGGSPPDYHVLPVGNAGNITAYWKGYVEYHERGEID
                     RKPVMLGFQAAGSAPIVENRRIDKPQTIATAIKIGDPASWKGAVNARDESGGRIEAVT
                     DDEITNAQMLMARTEGVFAEPASAASIAGVIKLAKRGFFKGGERVVCILTGHGLKDPD
                     RAIAVTEPPVRVESKLDTVLDML"
     gene            complement(1069..>2211)
                     /locus_tag="ENN56_01695"
     CDS             complement(1069..>2211)
                     /locus_tag="ENN56_01695"
                     /inference="COORDINATES: protein motif:HMM:PRK06349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDS74232.1"
                     /translation="PLPDGVLSDDVDGLLNDPNIDIVIELVGGTGFARTVVLTAIENG
                     KHVVTANKALLATHGEEIFRAAREKGVDIAFEAAVGGSIPILKALRESLSTSRIDAVY
                     GIVNGTTNYILTRMVNEGAPYDELLRSAQEQGFAERDPSADVKGWDSQQKLTILMRLA
                     FGTVVRPDDILCEGIEQITPLDIDYAREFGYTIKLLAIAKRRGDKLEARVHPAMIPVK
                     TLIADVQYEYNAIEVIGDEFGTQVFYGKGAGRRPTATVVVSDALDIAQRIQSGAPCSR
                     VREIIDRKDAPTLVPSDTLSMRHYVRLEVTDHAGVLEDIAHVFASENISIESVIQKGR
                     ATADTVPLVIMTHEAEEAAVSRAVGQLEELDAVRPPVQRIRIEDLL"
BASE COUNT          421 a          667 c          618 g          505 t
ORIGIN      
        1 ccagcatgtc gagtactgta tcgagttttg actcgacacg tacgggtggc tccgtcacgg
       61 cgattgcgcg atccgggtct ttcaagccat gaccggtgag gatacacacg acgcgctcgc
      121 cacccttgaa gaagccgcgt ttcgccaatt tgatcacacc ggcaatgctc gccgcgcttg
      181 ccggttccgc aaacacacct tccgtccgcg ccatgagcat ctgtgcgttg gtaatctcgt
      241 cgtcggtcac cgcttcgatt cgcccgcccg attcgtcacg tgcgttgacc gcgcccttcc
      301 agctcgccgg atcaccaatc ttgatcgccg ttgcaatggt ctgtggtttg tcgatacgtc
      361 ggttctccac gatcggcgcg gacccggcgg cctggaaccc aagcatcacc ggcttacgat
      421 cgatctcgcc gcgctcgtga tattcgacgt agcctttcca atatgccgtg atatttccgg
      481 cattgccgac cggaagtacg tggtaatcgg gcgggcttcc accaagctga tcgcagattt
      541 cgaacgctcc ggttttttgc ccttcgattc ggaacggatt gatcgagttc accacggcga
      601 ccggacgtgc gtcagccact tctcggacaa gccgaaggca gtcgtcaaag tttccgtcga
      661 tctccatgac gagggcaccg tgcatcatcg cttgcgcgag cttcccttgc gcgatgttcc
      721 cgcgcgggat gaataccacg ggaatcatac tggaccgcgc cgcgtacgcc gccgccgacg
      781 cactggtatt tccggtcgac gcgcacacaa cgatctcggc acccgattcc ttcgcttttg
      841 agatcgcgag tgtcatgcca cggtccttga acgacccggt cgggttcatc ccctcgaact
      901 tgaggtataa ttcgaggtct acaccgatct cattcggaag gcggaccgcc ggaatgagcg
      961 gcgtattgcc ttcaagtaac gtaatgaccg gcgtggagtc ggtgaccggc aagtattcgc
     1021 gatactgttc gataacaccg cgccatggct tgttacggtt ctgcgtgctc ataggagatc
     1081 ctcgattcgt atccgttgga cgggcggacg aacagcgtcg agttcttcga gttgacctac
     1141 cgcacgggaa accgcagcct cttccgcttc gtgtgtcatg attaccagtg gaaccgtatc
     1201 cgccgtagca cgacccttct gaatcaccga ctcaatgctg atgttctcgc ttgcgaagac
     1261 atgtgcaatg tcctccaaaa cgcctgcgtg gtctgtgact tcaaggcgta cgtaatgacg
     1321 catcgagagc gtgtccgagg gaacgagcgt cggtgcatct ttccggtcaa tgatctcgcg
     1381 gacgcgtgaa cacggcgcgc cactctggat tcgttgcgcg atgtcaagcg cgtccgatac
     1441 gacgacagta gccgtcggac ggcgccccgc gcctttcccg taaaacacct gtgtgccgaa
     1501 ttcgtcgccg ataacctcga tcgcgttgta ctcatactgc acatcggcaa tgagcgtttt
     1561 taccggaatc atcgccgggt gaacacgcgc ttcaagcttg tcaccacgtc gtttcgcgat
     1621 tgccagaagt ttgatcgtgt atccgaattc acgcgcgtaa tcgatgtcga gcggagtgat
     1681 ctgctcgatt ccttcgcaga ggatgtcatc aggacgaacg acggtgccaa atgcgaggcg
     1741 catgaggatc gtgagcttct gttgcgaatc ccaccccttc acatcggcgc ttggatcgcg
     1801 ttcggcgaac ccctgctcct gcgcgctgcg gagtagctcg tcgtacggtg caccttcgtt
     1861 caccatgcgg gtgaggatgt agttcgtcgt tccgttgacg ataccgtaaa ccgcgtcgat
     1921 gcggctggtc gataggcttt cgcgaagcgc cttgaggatc ggaatactcc ctcctacagc
     1981 cgcttcaaag gcgatatcaa cacctttctc acgcgcggcg cgaaagatct cttcgccgtg
     2041 cgtcgcgagg agcgctttgt tggcggttac gacgtgcttc ccgttttcaa tcgcagtcag
     2101 tacgaccgtt cgtgcaaagc cggtgccgcc gacgagttcg atgacgatat cgatgttcgg
     2161 atcgttgaga agaccgtcga catcgtcgga caaaacgccg tcaggtagcg g
//
LOCUS       DSCC01000143            2174 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034374, whole genome shotgun sequence.
ACCESSION   DSCC01000143 DSCC01000000
VERSION     DSCC01000143.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034374"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="ENN56_01700"
     CDS             complement(<1..1223)
                     /locus_tag="ENN56_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74233.1"
                     /translation="MIVPSSSAGRTSDTTGRVVIIGAAVLGIAARVGVLVWHGNPSDP
                     NLWEFGTIARNLVEHGTFAFYHPAAASAYMPPGFPLIIAGLYTVFGIGHAAHWILAAM
                     LLTAGIALPFVVRRIAAHVWDHDVAAVAFVFAMLWPHLILMNAGFTGLPVSTLLLALA
                     VTVAIDVHANGLGSVAACGLLLGGYSLFRFEGLLFVLPIGYLVFRRMSNRNVKARIGV
                     LAVLIALTITPLVPWVARNYAVFGTPLLSTSAGFNLARGHNAITVGTGRDPRTGEWAE
                     LLGNPPVALDDIDESADELARDRYFRAAAIEYARSHLVRELTLTARKAFFFLVADFTH
                     PADRLLIVWTPTFVAFVLGFWYWIRKGRNDPAQQALWLVFAVQLGVAMVFFVVPRYRM
                     AVAWVPLLFFAAWYAR"
     gene            complement(1224..>2174)
                     /locus_tag="ENN56_01705"
     CDS             complement(1224..>2174)
                     /locus_tag="ENN56_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDS74234.1"
                     /translation="WFEPERVRRGTPLQTEHPEWLLENGEDKGNLLFDLGNDEARRYL
                     TGFISGMIEEVGIDIYRQDFNFEPLTYWKRADEPDRVGMHEIRHIEGLYAFWDELRAR
                     HPNLLIDNCSSGGRRIDLETTSRSFPLWRSDYTDLPAKNEGMKLQIGAQAQTAGLSRW
                     VPLHSASVWTFEPYDMRSSFSTGVSLYTDILADDYPIDLVKHAVAEVKRLRPYLLGDF
                     YPLVLLTVEEHDWCAYQFDRPDLGTGCAIFLRRHESPYPSVVAGLRNIDTDARYEISL
                     SRVYEHGSFHQIGAWDLKRLTVSIPTAPGSLLLEYRKRDG"
BASE COUNT          506 a          648 c          617 g          403 t
ORIGIN      
        1 cgcgcatacc acgcagcaaa aaacagaagc ggcacccacg ccacagccat ccggtagcgc
       61 ggtacgacga agaacaccat cgcaaccccg agttgcacag cgaacacaag ccacagagcc
      121 tgctgcgctg ggtcgtttcg tccctttcgt atccaatacc agaatcccaa cacaaacgcc
      181 acgaacgtag gggtccatac aatgagcaat cggtccgccg gatgcgtaaa atccgcgacg
      241 agaaagaaaa acgcctttcg cgcggtgagt gtgagttcgc gaacgaggtg tgatcgcgcg
      301 tattcgatcg ccgcagcccg gaagtatcga tcgcgtgcga gttcatcggc cgactcgtca
      361 atgtcgtcca gcgcgaccgg tggattcccc agaagttccg cccattcacc cgttcgcgga
      421 tctcgtccag tacctacggt gattgcattg tgtccacgcg cgaggttgaa tccggcggac
      481 gtgctcaaca gcggcgtccc aaataccgcg tagtttcgcg ccacccacgg gacgagtggc
      541 gttatcgtta gcgcgatcaa aacagcaagt acgccgattc gcgccttaac gttgcggttc
      601 gacatccgac ggaatacgag ataaccgatt ggcaggacaa acaggagccc ttcgaatcga
      661 aacagcgagt atccaccgag cagtaaaccg cacgcagcga cggagccaag gccattcgca
      721 tggacatcga ttgcgacggt gactgccaga gcgagcagga gcgtcgaaac cggaagtccg
      781 gtgaagccgg cgttcatcag aataagatgc ggccagagca tcgcgaacac gaatgcgacg
      841 gcggccacgt cgtggtccca cacgtgcgct gcgattcgcc gcacaacgaa cggtagcgcg
      901 atacccgctg tgaggagcat tgcagcgagg atccaatgtg ctgcgtgccc gatgccgaat
      961 accgtgtaca gccccgcaat gatcaacggg aacccgggtg gcatgtaggc cgatgcagca
     1021 gcgggatggt agaacgcaaa cgttccgtgc tcaacgagat tccgcgcgat ggtcccgaat
     1081 tcccacaggt tcggatcgga cgggttccca tgccatacga gcacaccgac acgcgcggca
     1141 ataccgagaa ccgctgcgcc gatgataact acacgcccag tggtgtccga tgtgcgaccg
     1201 gccgatgagc tcggtactat cattcagcca tctcgtttgc ggtattcgag aagcagcgaa
     1261 cccggtgcag tcggaatcga tacggtcagt cgcttcaaat cccacgcgcc gatctgatgg
     1321 aatgagccgt gctcgtatac acggctcaag ctgatttcgt atcgtgcgtc cgtatcgata
     1381 tttctgagcc cggcaaccac tgacgggtac gggctttcgt ggcgtcggag aaagatcgca
     1441 cagccggtac cgaggtctgg tcggtcgaac tgatacgcac accaatcatg ctcttcgaca
     1501 gtcaacagca cgagcgggta gaaatcgccg aggagatacg gacgaagccg cttcacctcc
     1561 gcaaccgcgt gcttcacgag atcgatcggg tagtcatcgg cgaggatatc ggtatagagc
     1621 gatacacccg tcgaaaacga actgcgcata tcgtacggtt cgaacgtcca caccgacgcg
     1681 gaatggagcg gcacccaacg cgagagtccg gctgtctgcg cctgtgcgcc gatctggagt
     1741 ttcattccct cgtttttcgc ggggagatcg gtgtagtcgc tccgccaaag tgggaacgat
     1801 cgcgaagtcg tttccaagtc aatacggcga cccccgctcg agcagttgtc gatgagcagg
     1861 ttcggatgac gagcgcgtag ttcatcccag aacgcataca gaccttcgat gtggcgtatc
     1921 tcatgcattc cgactcgatc aggttcgtcg gcacgcttcc agtaggttag cggttcgaag
     1981 ttgaaatcct gccgatagat atcgatccct acctcttcga tcatgcccga aatgaaaccc
     2041 gtcaagtagc ggcgagcttc gtcatttcca aggtcgaaca ggagattccc cttgtcttcg
     2101 ccattttcca gcagccactc cggatgctcg gtctgcaacg gtgttccgcg acgtacgcgt
     2161 tccggctcga acca
//
LOCUS       DSCC01000144            2520 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028176, whole genome shotgun sequence.
ACCESSION   DSCC01000144 DSCC01000000
VERSION     DSCC01000144.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028176"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="ENN56_01710"
     CDS             complement(<1..1342)
                     /locus_tag="ENN56_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HDS74235.1"
                     /translation="MASTDPKRPNVVFILSDDQGAWALGCAGNSEIRTPNLDSLAANG
                     TRFENFFCASPVCSPARASILTGRIPSQHGVHDWIRDGNMLSDVEWGAQGKVIEYLRG
                     IPGYTDVLAANGYTCGISGKWHLGDSGHPRKGFSSWKVHSRGGSGYYDAPMFADGREY
                     VEPRYVTDVIAENGVAFLREQVNSDAPFYLSVHYTAPHSPWTPDQHPSETYDDYHANC
                     PFHSTPFEPMHPWQIASAPSGYDEVSRRRELSGYYTAVTEMDHTIGDIVAEIDRMGIR
                     ENTLIIFTGDNGMNMGHHGLYGKGNATFPANMFDTSVKVPMIISQPGRVPSGVVCEEM
                     LSHLDIMPTLLDYLGMENPVADEIPGASFAPILHGEKLTGRDAVVVYDEYGPTRMIRT
                     REWKYVHRYQYGPHELYNLKDDPDERVNLIDADGMSGVARDLRARLAEWFVHWAD"
     gene            complement(1390..2433)
                     /locus_tag="ENN56_01715"
     CDS             complement(1390..2433)
                     /locus_tag="ENN56_01715"
                     /inference="COORDINATES: protein motif:HMM:PF13088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74236.1"
                     /translation="MFAYTRFTGGRDDAATADIAEIHSDDGGKTWSDPVIIVPNRGEV
                     NTMSVSLLRMNDGRILFCYLTKDGIHGWPHGNCRPWVCFSSDEGATWTEPKLITQVPG
                     YYVQNNDRIIQLSDTHPVAPGRLIAPQSFHRLTGYEPEIEKFHFTSSIVIWTISDDGG
                     ETWREAKTWWAIPVPSGSGLQEPGVIERPNGKLYGWARSDTGYQWTTESGDGGETWAP
                     FQPERTFMSPNSPLCMKRIPGTDRLLVIWNDRSDRWDIGTPDKWSWDRTPLTSAISDD
                     NAATWTRHHLIEDDPKHGYCYTAIHFLSAEEAHENESFVLLAYCAGGEETKGVLNRLR
                     MRRIPLSWFTAES"
BASE COUNT          484 a          783 c          726 g          527 t
ORIGIN      
        1 gatcggccca gtgcacgaac cactccgcca atcgagcacg cagatcacgc gcgacgccgg
       61 acattccatc cgcgtcgatg agattcaccc gctcatccgg atcgtccttc agattgtaga
      121 gctcgtgcgg accgtattgg taccgatgga cgtacttcca ctcgcgcgtg cggatcatac
      181 gggtcgggcc gtactcgtcg tataccacca ccgcgtcacg ccccgtgagc ttctcgccgt
      241 ggagaatcgg cgcgaaactc gccccgggaa tctcgtcagc aaccggattc tccataccga
      301 ggtagtcgag cagcgtcggc atgatgtcga ggtggctgag catctcctcg cacacgacgc
      361 cactcggaac acgacccggc tgcgagatga tcatgggcac tttcaccgat gtatcgaaca
      421 tgttcgcggg gaaggtcgcg ttgcctttcc cgtaaaggcc gtgatgcccc atgttcatgc
      481 cgttatcgcc cgtgaagatg atgagcgtgt tctcccggat ccccatgcgg tcgatctccg
      541 caacgatatc cccgatggtg tgatccatct ccgtgacagc ggtatagtaa ccgctgagct
      601 cgcggcgacg gctcacctcg tcgtatcccg acggtgcgct cgcgatctgc cacgggtgca
      661 tcggctcgaa cggcgtcgag tggaacgggc agtttgcgtg ataatcgtcg taggtctcgc
      721 tcgggtgttg atccggagtc cacgggctgt gcggcgcggt gtaatggaca ctgaggtaga
      781 atggcgcgtc tgaattcacc tgctcgcgga gaaacgcgac tccgttctcg gcgataacgt
      841 cggttacgta gcgtggctcg acatattcgc gcccgtcggc gaacatcggt gcgtcgtaat
      901 agccgctccc gccgcgtgag tgaaccttcc aagaggaaaa gcccttccgc gggtgccccg
      961 aatcgccgag atgccacttg ccgctgatac cgcacgtgta tccgttcgcc gcgagcacat
     1021 cggtgtatcc ggggatgccg cggagatact cgatcacctt cccctgggca ccccattcaa
     1081 cgtcactcag catgttcccg tcgcggatcc aatcgtgtac gccgtgctgc gagggaatcc
     1141 gtccggtgag gatactcgcg cgagcgggag agcagaccgg agacgcgcag aaaaagttct
     1201 caaaccgcgt gccgttggca gcgagcgaat cgagattcgg tgtccgtatc tcggagttgc
     1261 ccgcgcaacc aagcgcccat gcaccttggt cgtccgaaag gatgaacacc acgttcggac
     1321 gtttcggatc agtcgatgcc atggtcagtt ctccttcgag actgctctct cgccccgcga
     1381 acgttgttct tacgactccg ccgtgaacca actcaacggt atccgacgca tgcgaagtcg
     1441 gttcagcact cctttcgtct cttcaccgcc cgcgcaatac gcaagcaaca cgaacgactc
     1501 gttttcgtgt gcctcttccg cgctgagaaa atgaatcgcg gtgtagcagt agccgtgttt
     1561 cggatcgtct tcaatgagat gatggcgtgt ccacgtcgcg gcgttatcgt cgctgatcgc
     1621 gctggtgagc ggcgtccggt cccagctcca tttatccggc gtgccgatat cccaacggtc
     1681 ggagcgatcg ttccagatca cgagcagacg gtcggttccg ggaatccgct tcatacaaag
     1741 gggcgaattg gggctcatga aggtgcgttc cggttggaac ggcgcccacg tttcaccgcc
     1801 gtcgccggac tcagtcgtcc attgataccc ggtatccgaa cgcgcccagc cgtaaagctt
     1861 gccattcgga cgctcgatga ctcccggctc ctgaagaccc gatccactcg gaacgggaat
     1921 cgcccaccac gtcttcgctt cacgccatgt ctcgccaccg tcgtcggaga tcgtccagat
     1981 cacaatgctc gacgtgaaat ggaacttctc gatttcgggc tcatacccgg tcagccggtg
     2041 gaacgactgc ggcgcaatga gacgccccgg tgcaaccggg tgcgtatccg agagctggat
     2101 gatccggtcg ttgttctgca cgtagtaacc gggaacctgc gtaataagct tcggctctgt
     2161 ccacgtggcg ccttcgtcac tgctgaagca tacccacgga cggcagtttc cgtgcggcca
     2221 gccgtggatg ccgtctttcg tcagatagca aaagaggatg cggccatcat tcatcctcaa
     2281 gagcgacacg ctcatcgtat tcacttcgcc gcggttcggt acaatgatta ccggatcgga
     2341 ccacgttttc ccgccgtcgt cggagtgaat ctccgcgata tcagcagtag cggcatcgtc
     2401 acgaccgccg gtgaaccgtg tgtacgcaaa catgacacgg gttccaccgt caggacggcg
     2461 cagcgtaatg aacgcgcctt ctgaatttcg tggattatcc ggtgttggct cgtattgaac
//
LOCUS       DSCC01000145            2196 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033908, whole genome shotgun sequence.
ACCESSION   DSCC01000145 DSCC01000000
VERSION     DSCC01000145.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033908"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>2196)
                     /locus_tag="ENN56_01720"
     CDS             complement(<1..>2196)
                     /locus_tag="ENN56_01720"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13432.4,HMM:PF13525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS74237.1"
                     /translation="YAQAAMGTGNVVPYSLYSLGWVRQKLEEYDGAIAAYQEMIRVAP
                     DHPLVPQSRFKMGESQYQLGQYREAIESLSESMPKISDPQLRANGHYLLGESYRSLGE
                     YDRARHHYRTVLSDYGRTSLAPAALYAIGWTHIEERDYREAVSTMSQLEEQYPDADVV
                     PQAKFRRALALKELNQPRETERVLTELLRSYSDSDVADDASYELGTLAYNRQDYSIAE
                     GSFRRLLEEHPNSDLRMQSTYMLGETMLAQGELDSANTYYTTIIGSYQSSPLVPQALF
                     QRGWTRYKLGRYADAIDDLEQLIRLWPEHTLVADAYYLVAEAHYALGDLESASKMYRV
                     LVDKYPTSSLVSDAQYGLGWTAFKREDWNGAIRHFDEAKRSASNPQVKADAAVRVGDS
                     YFNQGDFDRALVAYREASRFDAENPRPIYYAAQSLFHLNRLEEAIGTYRQITDRFPRS
                     DFADDAWFMIGWSRLRIDEYPAAIESYRYLTINYPQSDLAPQAQLGIGDAYYNSGRYT
                     DAVRSYEEILRRWPESTQVTAAISGIWDSYLREGRIQDAIVRTEGYITENPESPVSAQ
                     LLMKRGDWFYSAGRYEDAVETYRRITDEFADVDLPSSPLFWEATALAQLGRRQEAIDR
                     FRRVAESSGPLAADALERIGDMRMDLDEYRLALESYTEYDRRFPESNKRAVVLTKQGR
                     AHLLLGSDMAADQAFRRAANIGGESMAGAEARLELSRLRIRRGEYDQAR"
BASE COUNT          462 a          652 c          574 g          508 t
ORIGIN      
        1 cccgcgcttg atcatactca ccgcgacgaa tcctgagtct cgacagttcg agacgagctt
       61 ccgctcccgc catcgattcg ccgccgatgt tcgccgcgcg ccggaacgcc tgatccgcgg
      121 ccatatcact accgagcaac aaatgtgcgc ggccttgctt cgttagcacc actgcgcgct
      181 tgttgctttc cgggaaacgt cgatcatatt cggtgtaact ttcaagagcc aaacgatact
      241 cgtcgaggtc catccgcata tcgccgattc gttccaacgc gtctgcagca agcggcccgc
      301 tactctcggc gacacgacgg aatcgatcga tcgcctcctg tcggcgtccg agttgggcga
      361 gcgctgtagc ttcccagaag agtggcgaac tggggagatc gacgtcagcg aactcgtcgg
      421 taatccgccg atacgtctca accgcgtcct cgtaccgtcc ggctgaatag aaccaatcgc
      481 cgcgcttcat caatagctga gctgataccg gactctccgg attttcggtt atgtacccct
      541 ccgtgcggac aatcgcgtct tgaattctgc cctcgcggag ataactatcc cagataccgc
      601 taatagcagc cgtcacctgc gttgattccg gccatctgcg gagtatctcc tcatatgaac
      661 ggacggcgtc cgtgtatcgt ccgctgttgt agtacgcgtc acctatcccg agttgcgctt
      721 gtggcgcaag gtcactctgc ggatagttga ttgtaaggta tcggtacgat tcgatcgcgg
      781 ccggatactc gtcgatacgt aaccgcgacc acccgatcat aaaccatgca tcgtcggcga
      841 aatccgaacg cggaaaccgg tcggttattt gccgataggt accgatcgcc tcttcaagcc
      901 gattcaggtg aaacagactc tgcgccgcgt aatagatcgg tcttgggttt tcggcatcga
      961 accgggacgc ttcacggtag gccacaagcg cacggtcgaa atcgccttga ttgaaatacg
     1021 aatcgccgac tcttaccgcc gcatcggcct tcacttgagg attcgaagcg cttcgtttcg
     1081 cttcatcaaa atgccgaatc gctccgttcc aatcctcacg tttgaatgcc gtccaaccga
     1141 gtccgtattg cgcgtcggag acgagtgaac tcgttggata cttgtccacc agcacgcggt
     1201 acattttcga cgccgactcg agatcgccga gtgcgtaatg agcttctgca acgagatagt
     1261 atgcgtcggc aaccaacgta tgttctggcc agaggcgaat gagctgttca agatcgtcaa
     1321 tcgcatccgc gtatcggccc agcttgtatc gggtccagcc gcgctggaag agtgcttgcg
     1381 gaaccagcgg cgacgattgg taggaaccga taatcgtcgt gtagtacgta tttgcgctgt
     1441 caagctcgcc ctgcgcaagc atcgtttcac cgagcatata cgtcgactgc attctcaggt
     1501 cgctgttcgg atgttcttca aggagtcgac ggaatgatcc ctcagcgatg ctgtaatctt
     1561 gtcggttgta cgccagcgtt cccaactcat acgacgcgtc atcagcaaca tccgaatccg
     1621 aatacgaacg aagcagttcc gtcagcacac gttccgtttc tcggggttga tttagctcct
     1681 tgagtgccag ggcacgacga aacttcgcct ggggaacgac gtcagcatcc ggatattgct
     1741 cttccaactg actcatcgtc gaaaccgctt cacggtaatc gcgctcttca atatgcgtcc
     1801 agcctatcgc atacagcgca gcgggtgcaa gagaagttcg tccgtaatcc gaaagcactg
     1861 ttcggtaatg gtggcgcgcc cgatcatatt cgccgagtga ccgatagctc tcaccaagaa
     1921 ggtagtgacc gttcgcgcgc agttgcggat cgctgatttt gggcatcgat tcgctgagcg
     1981 attcaatcgc ttctcggtat tgcccaagtt ggtattgcga ttcgcccatt ttgaaacgac
     2041 tttgcggcac aagtggatga tccggcgcca cgcgaatcat ttcctggtac gcagcgatcg
     2101 caccgtcgta ttcctcaagc ttctgtcgca cccatccgag actgtacaag gaatacggca
     2161 cgacattccc ggttcccatc gcggcctgtg cgtaac
//
LOCUS       DSCC01000146            1524 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055595, whole genome shotgun sequence.
ACCESSION   DSCC01000146 DSCC01000000
VERSION     DSCC01000146.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055595"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..493)
                     /locus_tag="ENN56_01725"
     CDS             complement(<1..493)
                     /locus_tag="ENN56_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDS74238.1"
                     /translation="MNVLVINAGSSSVKYQLIDSARKKALAVGSVERIGMADAMLVHK
                     PDGREKVTVTGEILDHIQAIEYTLAILLSPQHGVIKDHSEIDAVGHRVVHGGEKFSAS
                     AIITSEVMAQIRDCIELAPLHNPHNVRGIEACQRLLPAATQVAVFDTAFHQTMPDFAY
                     LYAL"
     gene            787..>1524
                     /locus_tag="ENN56_01730"
     CDS             787..>1524
                     /locus_tag="ENN56_01730"
                     /inference="COORDINATES: protein motif:HMM:PF02056.14"
                     /note="catalyses the hydrolysis of terminal non-reducing
                     alpha-D-galactose residues in alpha-D-galactosides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDS74239.1"
                     /translation="MPKITFIGAGSLGFFKTLMIDCLSFDALRHADWAIVDIDEKRLG
                     YAHRIAKRIMGEAKSTGDLQVSTDRREVLEGSDYVIMAILVGGYEPIAHDVDIPMKYG
                     VDQCIADSHGPGGVFRALRTIPVKVDIARDIEELCPDAWVLSYTNPMSMLCRAMTSET
                     DINLVGLCHSVQGTTGMLADWVGAPKSEIDYLVAGINHQAWVLRIRHNGEDLYPRIHK
                     AANEPEHYKSLTASVEMLNHLGYFVTEG"
BASE COUNT          349 a          428 c          423 g          324 t
ORIGIN      
        1 gaagtgcgta caagtacgca aaatccggca tggtctgatg gaaagccgta tcgaacaccg
       61 caacctgcgt cgccgccggc agaaggcgtt gacacgcttc gattccgcga acattgtgcg
      121 gattgtggag cggcgcgagt tctatacagt cgcggatttg cgccattact tcgctcgtga
      181 tgatcgccga tgcgctgaac ttctcaccgc cgtgaaccac acgatgtccg acggcgtcga
      241 tttccgaatg gtctttaatg actccgtgct gcggactgag caaaatcgcc aacgtgtatt
      301 caatcgcctg aatgtgatcg agtatctcgc cggtgacagt gaccttttcg cgtccgtcgg
      361 gcttgtgaac gagcattgcg tcggccatgc cgatacgctc gactgaacca acggcaagcg
      421 ccttcttccg tgctgaatcg atgagttgat acttgaccga cgagcttccg gcattaatta
      481 cgaggacatt catggtatcg gggctccgtt ccggggggat atatgggacg ggatcatagc
      541 atttacacag tggcgcgtta atgtcgatcg tcgcagatcg tcatgtgcga caacatgggt
      601 gaaaaagcgc aaccggaata gtgaaaatac cgtgtttgtg tgcaggttca aagcgggttc
      661 cgcaccaata cggtaaggaa atggtcagac tcccggtgtg aataccgctt gccccaattc
      721 cgagtttcgg gtattcattt ggttcctcaa aaaagcatcc gatccacgta ccttccggag
      781 gtattcatgc caaagatcac attcattggt gcgggaagcc tcggcttctt caaaacgctc
      841 atgatcgact gtctgagctt cgacgcactt cgtcatgcgg actgggcgat cgtcgatatc
      901 gacgaaaagc gcctcggata cgcgcatcgc atcgcgaaac gcatcatggg cgaagcgaaa
      961 tcgacaggcg acctccaggt cagcaccgat cgacgcgaag tcctcgaagg ttccgactac
     1021 gtcatcatgg cgattctcgt cggcggatac gagccgatcg cgcacgacgt cgacatcccg
     1081 atgaagtacg gcgtcgacca atgcatcgcc gattcgcacg gtccgggtgg tgtgttccgc
     1141 gcccttcgca cgattcccgt gaaagtcgat atcgcccgcg acatcgaaga gctctgcccc
     1201 gatgcgtggg tattgagcta cacgaacccg atgagcatgc tgtgccgcgc catgacgagt
     1261 gaaaccgaca tcaacctcgt cggtctctgt cacagcgtac agggcacgac cggcatgctc
     1321 gccgactggg tcggagcacc gaagagcgag atcgactatc tcgtcgccgg gatcaaccat
     1381 caggcgtggg tactccgcat tcgtcataac ggcgaagatc tctatccgcg cattcacaaa
     1441 gccgcgaacg aaccagagca ctataagagc ctcacggcga gtgtcgaaat gctgaatcac
     1501 cttggttact tcgtcaccga aggc
//
LOCUS       DSCC01000147            1574 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053313, whole genome shotgun sequence.
ACCESSION   DSCC01000147 DSCC01000000
VERSION     DSCC01000147.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053313"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..905
                     /locus_tag="ENN56_01735"
     CDS             <1..905
                     /locus_tag="ENN56_01735"
                     /inference="COORDINATES: protein motif:HMM:PF13439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDS74240.1"
                     /translation="AAVIHLVREIRERKVDIIHTHLVRAGIVGKLAASIVRVPVVTTR
                     HYTRDAKSRRWDFVAEDVLTRLLADRVIAIGDSVFDTIREQKMCAPEKIVLHRNAIDV
                     ASYVCQGRDERTPNLVIGTVGRLHPAKAHEVFLDALAMVRRTHPEVTGVIVGGGERLV
                     ALKEYADRCGVLDAVTFTGAVDSDTVRDWLHRFDIFVLPSDWEGLPISLIEAAAAGLP
                     IVATDVGGCPEVVRDGENGFLVPPRDPAAVANAVERLIRDVSLRDAFGNRAVEIAKSE
                     FDIHRLADQTADLYREIRMRKACT"
     gene            896..>1574
                     /locus_tag="ENN56_01740"
     CDS             896..>1574
                     /locus_tag="ENN56_01740"
                     /inference="COORDINATES: protein motif:HMM:PF13522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74241.1"
                     /translation="MHVMCGIAGVAMRAAPVDAALIGRITERLYRRGSDDSDVVVHGK
                     VGLGHRRLSVIDTSSAGRQPMGTEDDRYWITYNGETYNFADLREDLEKRGERFHSRTD
                     SEVILRAFRHYGRETWVRLSGMFALAIYDRQTGEVVLSRDRYGIKPLYYAVLPDRLVF
                     ASELKAVVVDPAVPRDLDDEAIDLYLTFGYIPAPWTIYRHVCMLMPGHDALWDGNTLS
                     TRQWYDLA"
BASE COUNT          336 a          418 c          471 g          349 t
ORIGIN      
        1 aagccgccgt gattcacctc gtccgtgaga ttcgcgaacg aaaggttgac attattcaca
       61 cgcacctcgt tcgtgcaggt atcgtcggga agctcgccgc gagtattgtg cgcgttccgg
      121 tcgtaacaac gaggcattat acgcgtgatg cgaaaagtag acggtgggat ttcgtcgctg
      181 aggacgtgct cacgcggttg cttgcagacc gtgtaatagc aatcggcgat agtgtgtttg
      241 atacgattcg cgaacaaaaa atgtgcgcac cggagaagat tgtcctccac cgaaacgcga
      301 tcgatgttgc gtcatacgtg tgccaaggca gggacgagcg gacaccgaat ctcgtgatcg
      361 gaaccgttgg gcgattgcat ccggcgaagg cgcacgaggt gtttctcgat gcgctcgcga
      421 tggttcgacg cacccatccg gaagtgacgg gagttatcgt tggcggtggg gaacgacttg
      481 tcgcgcttaa ggagtacgcg gaccggtgtg gtgtgttaga cgcagtgacg tttaccggag
      541 ccgtcgattc ggataccgtg cgtgactggc ttcaccggtt cgacatcttt gttcttccct
      601 ctgattggga aggtcttccg atatcgttga tagaagccgc tgcagcggga ttaccgattg
      661 ttgcaaccga cgttggtggc tgtcctgaag tagtgcgaga cggagagaac gggtttctcg
      721 ttccaccgcg tgatcctgcc gcagtggcaa acgccgtcga gcgcttaatc cgagatgtaa
      781 gccttcgtga cgcgtttggg aatcgagccg ttgagattgc gaagtccgag tttgacattc
      841 accgattagc agaccagacc gcggacctgt accgggaaat acgtatgaga aaggcgtgca
      901 cgtgatgtgc ggtatcgccg gtgtcgcaat gagagccgct cccgttgacg ccgcactcat
      961 cgggagaatc accgaacggc tgtaccgtcg cgggtccgac gactccgatg tggtggtaca
     1021 cggcaaagtc ggactcggcc atcgacggct gagcgtaatc gacacatcga gtgccggacg
     1081 ccaaccgatg ggtacggaag acgatcgata ttggattacg tacaacggcg aaacatacaa
     1141 cttcgccgac cttcgcgagg acctcgaaaa gcgcggtgaa cgatttcaca gtcggaccga
     1201 ttcggaggtg attcttcgag cgtttcgcca ttacggacgc gaaacatggg tgcgtctctc
     1261 cggaatgttc gcgctcgcga tctacgatcg acagaccggt gaggttgtct tatcgcgcga
     1321 ccgctacggt ataaaacctc tctactatgc cgttcttcct gatagactcg tattcgccag
     1381 tgaattgaaa gcggtcgtcg ttgatcccgc cgttccccgc gacctcgacg acgaagcgat
     1441 tgatctctac ctgacctttg gatacatacc cgctccgtgg acgatatacc gccatgtgtg
     1501 catgctcatg cccggtcacg atgccctgtg ggacggcaat acactgtcca ctcgccaatg
     1561 gtacgacctt gcac
//
LOCUS       DSCC01000148            2802 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024511, whole genome shotgun sequence.
ACCESSION   DSCC01000148 DSCC01000000
VERSION     DSCC01000148.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024511"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..620)
                     /locus_tag="ENN56_01745"
     CDS             complement(<1..620)
                     /locus_tag="ENN56_01745"
                     /inference="COORDINATES: protein motif:HMM:PF07715.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74242.1"
                     /translation="MLPRLRSCWFLCCVLSLFMLVFLASRAFSATTGIISGIVADDSG
                     MPIVGAAVQVEGTRLGATSDADGQYVILQVPPGEYTVTARLIGFNVTSVTDVRVSADL
                     TTRVNFRLYQEAIEIEAVVVTASRDPIRIDVTSSQTTIDAQRVSEMPVNQMLNVLNYQ
                     PGVSVVRGNELEIRGGGPSEIRFQVDGVDRTDGLTGKGYTQLNQVLV"
BASE COUNT          652 a          792 c          718 g          640 t
ORIGIN      
        1 acgagcacct ggttcaactg cgtatacccc ttgccggtga ggccgtcggt acggtctacg
       61 ccgtcgactt ggaaacgaat ctctgatggt ccgccgcctc gaatctccaa ttcattgccg
      121 cgaacgacac tcactcccgg ctgataattc aacacgttca gcatctgatt aaccggcatc
      181 tccgatacgc gttgggcatc gatagttgtc tgcgaactcg ttacatcgat gcgtatcggg
      241 tcgcgagatg cagtgacaac cacggcttcg atttcaatcg cttcctgata caaacggaag
      301 ttcactcgcg tcgtcaggtc cgcgcttaca cgcacgtctg taacgcttgt gacgttaaat
      361 ccgatgaggc gcgccgttac cgtgtattcg cctggaggaa cctggagaat gacgtactga
      421 ccgtcggcgt cagaagtcgc gccgaggcgg gtgccttcca cctgtacggc agcgccgaca
      481 atcggcatac cgctatcatc ggctacaatt ccggagatta taccagtggt tgcggagaaa
      541 gctctactcg caaggaatac cagcataaat agggaaagaa cgcaacacag aaaccaacag
      601 gaccgtagac gaggaagcat ggtgacctcc ctctttcgca gaaatccgag ggtcgcgact
      661 tttcgaccgc atcgaaatac gtcgcagcgg tgtacactcg ggtgtgctca gactgtcatc
      721 gtcgtatatg accccaatct atatgatcaa ccaaccgact taacgaatga gccccgttct
      781 caaaaagtaa ggagagtatc ctacgtctct tgcgacgcta atatatctca tacacatgac
      841 ggcgccaaca tacacgccgc gttttttccg acacacaagc cttcttaaac gacgaagacg
      901 aggtgtcaca ttcacgtaac acctcgtctt ctcaccgaag aattgcgcga cgttatatct
      961 cgaatcgaac tccgaagcgg tagaagcgtg gattcataaa ctgaacccag tccgtttctg
     1021 cgctgattgg acgcagccag agagaggcat tcggatcgat tttatacggc tctgtgagca
     1081 cgtagaacgt cgacttgtca cctacctgta catcttcgcc aaggttgcgt cgtgtgtaga
     1141 tatacccaag gtagtcctgc cagttccgaa cgccatattt gttgagatgc ttcgtattga
     1201 gaacgttcag aatatcgaga tacgcggtca cgaaccgacc gttcgaaagc ggtatctgct
     1261 tcgagaattt catattcacg ttctgcgtgc taacccacgg aaggtagttc tcctccgtta
     1321 gctcacgcgc aggcagtatg ttggggttgt atatctcttc tccccccttc tgccaggagt
     1381 attccgttcc gagcgaccat ccgccggacc acgtacccca gtccttcggc gtgccgaggc
     1441 gcaagaatac tcggaagaaa ccacgggcag gctgtctccg cactgttggg agatacgggg
     1501 taaggaaata cttcatttcg ttaccaactg tcggtacgga aacttccccg gtccgtgtcg
     1561 aatacgattc atacgtaatc gcaccgttca cgaaacggcc cgtattccga gcaatctgga
     1621 actcgtaccc gcgaacatct tcatagttcg cgttccgata cgcgttatac gcaaagacac
     1681 caccgtttac gaccatcgcg gtgacctgat cctcgttgta cttctgatat ccggtcacct
     1741 tgaacacggt ctcatacggt aaaacgtgct ccactccgac ttcgtacatt gtggtcgtgc
     1801 tcggacgcag attcgcattg tacatgtagt tgatacgtcc gctgccaccg ccggggcgac
     1861 cgtcatgaat ggtgaatcca tacatgaacg tcggcttctg catcgcgtag aagatgccgt
     1921 agttgaagaa gaacttcgta cggttgctca ctggatgcga gataccgaag cggggtgctg
     1981 ctctccagta aactttgtta tcgcgcgaag gccatgcacg aacaacatcc cacggcatcg
     2041 gtgcggtgcc gttcaagtaa tccggaccgt tgatggtgta gaagttcgtt ccaatagaac
     2101 caagaagcga cgaactcgaa tcgagcccgg cctcgtggaa tgccagagcg aacatgtgac
     2161 gcgagatcgg ctggctgttc gtctgcccaa atacggtcga atcaaacata ttgtggtaga
     2221 accacgctgg atgccccccg tcgaagctct cgagacgaac tccgacgttc gcgatcatgc
     2281 catccgactc gtacttatcc tgaatgtatg cgccgaggat tttcggttgc gcggcgggat
     2341 actcgttccc atagaaacgg aatgtggtgt tccgaccagc gccaacacct gactcgaagt
     2401 atccacccga ctgttctttc aggtagtcga gatcacgacc gatgtactcg agaccggtct
     2461 tcaacgtgtg ctgaccgacg acgtgggtta ggtccccacg aatcgtggtc tgcgaaccac
     2521 caccttggat gttctcctcg ccgccgccac cgatagtgaa gcgacttgcc agatcaccat
     2581 atgggtttgc gacccaacct tgcggtgaaa cgatcgtcac gttgaatgtc gtgtcgtcag
     2641 tcgcgtccgc ccggtcacca cgtgcgggcg cccacgagcg aggtccccac gtaaacgtct
     2701 tcgtgatgaa ctttgtctcc gtcggaggcg ccgcgtacgc atttgggtga gcgtatggtg
     2761 aacgagtgag gaagctatcc ggattcgtcg cgtacgacat ac
//
LOCUS       DSCC01000149            2300 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031784, whole genome shotgun sequence.
ACCESSION   DSCC01000149 DSCC01000000
VERSION     DSCC01000149.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031784"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            372..1709
                     /gene="rlmD"
                     /locus_tag="ENN56_01750"
     CDS             372..1709
                     /gene="rlmD"
                     /locus_tag="ENN56_01750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDS74243.1"
                     /translation="MVESVTADSLPSVGDVVELDIADQAYGPDSVARLNGIAVFVPDT
                     APGDRVRARITEVHKRYLRARVVSILRESSKRVPYNCPIAHQCGGCQWQYLAYDEQLR
                     AKERAVRDALVRIGGWADPDVRAIIASPREWRYRNKARYWVETAGDQRRIGYYARSGD
                     AVVPIETCPLNMDGVDTTLTTVGRLIASETHRDVAESLESVIARESLGSGEKAVRLIL
                     RREVPVRAFAEALMREVPALVGVTYNRAGRNAMRRDRVVVGSGTLTEYVGALEYRVSV
                     DSFFQINPFATPLLVDVVREAAALDGVESVLEAYGGVGLSGVALAERASRVTLVEADR
                     SAANDARRIFSKRGIDSATVYERSVDDMGDLPGGVDVIVCDPPRQGAGRDGIAALNRH
                     GAYRLVYVACDPTALARDSAILRDAGWNLHWARPIDLFPQTYHVETVALFERR"
     gene            1714..2247
                     /locus_tag="ENN56_01755"
     CDS             1714..2247
                     /locus_tag="ENN56_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HDS74244.1"
                     /translation="MDSRTRRRPHSFVEPVDIDASRGDEGPRVNIVLVEPEIPQNTGT
                     IVRLAAATRCPLHLVEPLGFELSERAIRRAGLDYWQHASVTTYPSYKALRDAHPDGRF
                     WFTSKKAKRLYTSVTYRPDDFLVFGRETTGLPEEMIAADPERCIGIPMFGPVRSLNLA
                     NAVSVVVYEALRQLRGF"
BASE COUNT          416 a          634 c          779 g          471 t
ORIGIN      
        1 atctatgcgg tggcgctcgt cgcggtggtg ctcgtgatga acgcgctgcg tgggcagatt
       61 ccgcaggcgt tcggcttgac cccgattcgc gtcgatgcac tctcggttgc gcgtatatcg
      121 cccgattcgt tggatgcgtc ctcaccgcac gaggttgact acgcagtgac ggtgcgcatc
      181 gtcgggcgcg actcggtgac gacgccggtg cggctcgatc aattccgcgt cgataccgat
      241 gccgggcgat tccaaccgta cgcaagcgat gttctctttg acagcaccgg tatcctacac
      301 gtcgcaagag gcgatacgat cgtcggtgtg ctggtcttct cactgcccga gcgcgcggag
      361 ccaaagcagc tctggtggaa tccgtgaccg ccgactcgct cccgtcggta ggcgacgtgg
      421 tcgagcttga cattgctgat caggcgtacg gtccggactc agtcgcgcgg ttgaacggta
      481 ttgcagtctt cgttccggac acggcaccgg gcgatcgcgt gcgcgcacga atcaccgaag
      541 tgcacaagcg atatctccgc gcgagggtgg tttcgatact tcgcgaatca tcgaaacgag
      601 taccgtataa ttgccctatc gcccatcagt gcggcggttg ccagtggcaa taccttgcgt
      661 acgacgagca acttcgcgcg aaggaacgcg cggttcgtga cgcgttggtc cgcatcggcg
      721 gttgggctga tccggatgtg cgcgcgatca tcgcgagtcc acgcgagtgg cgctatcgga
      781 ataaggcgcg gtattgggtc gaaacggctg gcgaccaacg ccggatcgga tactacgcgc
      841 ggagcggcga cgcggttgtg cctatcgaga cgtgtccact taacatggac ggagtcgaca
      901 cgacgttgac gacagttggt cggctgattg cgagtgaaac gcatcgcgac gtcgccgaat
      961 ctctcgaatc ggtcattgca cgagagagtc tcggctcagg tgagaaagcc gttcggttga
     1021 tactccgacg cgaggtcccc gtgcgggcgt tcgccgaagc actcatgcgc gaggtgccgg
     1081 cgctcgtcgg cgtgacgtac aaccgcgcgg gtcggaacgc gatgcggcgt gaccgcgtcg
     1141 tcgttgggtc aggaacgctg acggagtacg tcggggcgct tgagtaccgt gtctctgtcg
     1201 attcgttctt tcagataaat cccttcgcga cgccactcct cgtggacgtg gttcgcgagg
     1261 ctgccgcgct cgacggcgtc gaatcggtgc tcgaggcgta tggcggcgtc gggctgtctg
     1321 gtgttgcgtt ggcagagcgt gcaagtcgag tcactttggt ggaggcggac cgcagtgcgg
     1381 cgaatgatgc gcggcggatc ttttcgaagc gcggcatcga cagcgcgacg gtatacgagc
     1441 gctcggtcga tgacatgggc gatctgcccg gcggcgtgga tgtgatcgtg tgtgatccgc
     1501 cgcgtcaagg ggctggacgg gatggaatcg ccgcgctgaa ccggcacggg gcgtatcggc
     1561 tggtctacgt cgcgtgtgat ccgacagcac tcgcacggga ttcagcgata ctgcgtgacg
     1621 ccggttggaa tcttcactgg gcgagaccga ttgacctgtt tccgcaaacg tatcacgtag
     1681 aaaccgtggc gcttttcgag cgtcgataag aagatggatt cacgtacgcg caggcgcccg
     1741 cacagctttg tcgagccggt cgatatcgac gcgagtcgtg gcgacgaagg tccccgagtg
     1801 aacatcgtac tggtcgagcc ggagattccg cagaatacgg gcacgatcgt ccgactcgct
     1861 gcggcgacga ggtgtccgct gcacttggta gagccgctcg gatttgagtt gagcgagcga
     1921 gcaatacggc gcgcggggct cgactactgg cagcatgcgt cggtcaccac gtatccttca
     1981 tacaaggcct tgcgtgatgc gcaccccgat ggtcggttct ggttcacgtc gaagaaggcc
     2041 aagcgtctgt acacgtcagt tacgtatcgg ccggacgact ttctcgtgtt cggacgcgaa
     2101 acgacggggc tgccggaaga gatgatcgcc gccgatccgg agcgctgtat cgggattccg
     2161 atgttcggac cggtacgcag tctgaatctt gcgaatgcgg tgtcggtggt tgtgtatgag
     2221 gcgttacgac aattacgcgg attttagcat catatgaaag gaatcgtggc accatgaagg
     2281 cgaaggttat cgaagtgcgg
//
LOCUS       DSCC01000150            3842 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1015936, whole genome shotgun sequence.
ACCESSION   DSCC01000150 DSCC01000000
VERSION     DSCC01000150.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1015936"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..325)
                     /locus_tag="ENN56_01760"
     CDS             complement(<1..325)
                     /locus_tag="ENN56_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDS74245.1"
                     /translation="MQTAPKGTRLHIGIFGRRNVGKSSVLNAFTRQEVSIVSETAGTT
                     TDPVEKAMELLPLGPVLFIDTAGIDDEGALGARRIERTRRAIDRTDIAVLVTDHAEIG
                     EFERHL"
     gene            487..1683
                     /locus_tag="ENN56_01765"
     CDS             487..1683
                     /locus_tag="ENN56_01765"
                     /inference="COORDINATES: protein motif:HMM:PF01408.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74246.1"
                     /translation="MSGSRTPHGVDTVEDVVIFQSLRSLMLTHATTVKRNFKRGSMAH
                     APITSRPVRGAIFGYGSIGPVHMMSVLGYTEDHTDRIPDVDIVAVAEPIAERVARVPE
                     GIKRVRDYQTAIQTIRIDVAHICLPHSMHAAAVIAAAEAGINIICEKPMALNAAEAQT
                     MLDAVKANGVRFSLISQNRLNPEKAWLKERVAAGDLGTIKRIDWEVDWFRSAAYYADS
                     GGWRGRDSDARGGALSNQAYHTLDLAMWLADAPVVEVTATTSIDRTVHPDIDVPDRLE
                     GELRFANGIRTKFLATVCGNPKDIIRLEVTGNRNSSAPMHIVVDATQIIEQNVAEETP
                     QFGGNGPVLGKACYGTSHQENIARSYEAFIDGTEVPVTAEDGIRVLRVIDAILESNGN
                     PVAISS"
     gene            complement(1734..3437)
                     /locus_tag="ENN56_01770"
     CDS             complement(1734..3437)
                     /locus_tag="ENN56_01770"
                     /inference="COORDINATES: protein motif:HMM:PF02423.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74247.1"
                     /translation="MDDFGVDDVHHQSGFLSADVRQRDHAAGMSCDVAIFGEYTDEVV
                     PRLKLRGVWRCKVLFRRLRKHLRGKLIDVTFIWIMHTDEFIRQIRCFVNAAVAVFPEI
                     IRDLFRRIRNVVFRRECLCLFIGQEIYRVDFLVKLDRIGFQALDNFVTAPFPQRELDT
                     DLVAHVCLPTDDSFYGVAIRAVEPERNTVHRLSIDRGTNAFFCIHRVYPLRENVHISV
                     LEIPSSGIRRKPNEERMTETERIHISDEEIEAIPLRQMIDAVTEALQALGRGEIDISP
                     RNEILGRKGDPDYLRIEMDAVWNRAGKPDWYCRKVIEESGARDTSGARVLGSRSAWIE
                     LRDTARNRVITLDAEVITNVRTGIAAALGSRWLSSNQSVTLAIIGTGRIAGATLRAAA
                     QVFDVHEVRVTSRSASNRTRFVRELSSELGMRILPFESVRECVRGADIVVAAVPSSNP
                     VVVSADVVDVPQITLVGGDPRVVLADSDVFSDRLVVVDSYEQALRTGDFVRTADDGFI
                     GSVRWAQVDGRPATLADASLGCLTSLCGEMTILVLTGIAALDLAVARLAWERRLRTQT
                     E"
     gene            2712..>3842
                     /locus_tag="ENN56_01775"
     CDS             2712..>3842
                     /locus_tag="ENN56_01775"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74248.1"
                     /translation="MNSFGFSHSLLVRLAPDSTRRYLQYRNMDILSQRIHAMNAKERV
                     RATINRQPVDRIPLGFYCADCDTVERVIGRKTYVRNKIGIQLALWEGRRDEVVESLKA
                     DSVEFYQKIDTVDLLTYKEAQTLPPKDYVPNPPKKIADDLWEDRNGRIYKASYLSNEL
                     VCVHDPNKRDVDEFTPEMFPEPSEEDLAPPDPSQFEAWDHFVSVFAKDRYIAGHSGGM
                     VALTHVGGQESGLMMYVINPEVVHAASRRAVIIAEARDTQVIRPEQDGVLFDQDMAGT
                     NGPMISPKLFREHCFPYMKQRVAHVKSLGQQILLHNCGDNRLLMPMFAEAGIECYQSL
                     QTNAGMTLENLQRDFSDHIVFWGGLAVENLIEGTPEDVRRNVR"
BASE COUNT          908 a         1150 c         1009 g          775 t
ORIGIN      
        1 gaaggtgtcg ttcaaactca ccaatttccg cgtgatcggt aaccaacacc gcgatatcgg
       61 tgcgatcgat tgcacgacgc gttcgctcga ttcgccgcgc accaagtgcg ccttcatcgt
      121 caatacctgc ggtgtcgatg aagagcaccg gtccaagtgg aagcagttcc atcgccttct
      181 caaccggatc ggtggtcgtt cccgctgttt cgctgacgat actcacttcc tgacgcgtaa
      241 acgcgttcaa aacagatgac ttcccgacgt ttcttcttcc aaaaatgccg atgtgcagcc
      301 tcgtgccttt cggtgccgtt tgcattcgac gatcctctgt ccctctgtaa gtacgtgcgc
      361 gaacgtgtct acgggtgcat cgcttctgcg cgtctgaaac cgtactcgcg ttgtatcata
      421 accccatcgg tcagtgaatc aacgcagttt cggcccgttg catgaacaac tgcaattcac
      481 tggccaatgt ccggttcccg aacgcctcac ggcgttgaca cggtcgaaga tgttgtcatc
      541 tttcaaagtc tacgatcgct gatgctaaca cacgctacta ctgtgaagag aaacttcaag
      601 agaggctcca tggcacacgc accgatcacg tcgcggcccg ttcgcggcgc gatcttcggt
      661 tacggttcaa tcggtcccgt tcatatgatg tccgtactcg ggtacacgga agaccacacc
      721 gaccgtatcc ccgacgtcga tatcgttgca gtcgccgaac ccatcgcgga acgcgtggca
      781 cgcgtacccg aaggcataaa acgcgtccgc gactaccaaa cagccattca aacgattcgt
      841 atcgacgtcg cgcacatctg cctgccgcat agtatgcacg cggcggcagt tatcgccgcg
      901 gcggaagcgg gtatcaacat aatctgcgaa aagcccatgg ctctcaacgc agcggaagca
      961 caaaccatgc tggatgctgt aaaagccaac ggcgtacgct tctcgctcat cagtcagaat
     1021 cgactgaatc cagaaaaagc ttggttgaag gagcgcgtcg cagcgggaga tttgggaacg
     1081 atcaaacgaa tcgattggga agtcgactgg ttccgcagcg ctgcatacta cgccgatagt
     1141 ggcggatggc gcggtcgtga ctcagacgcg cgcggcggtg cactttctaa tcaagcgtac
     1201 cacacgctcg acttggcgat gtggcttgcc gatgcacctg tggttgaagt taccgcaacg
     1261 acatcaatag atcgcaccgt tcacccggat attgatgtac cggatcgcct cgagggagag
     1321 cttcgcttcg cgaacggcat ccgaacgaag ttcctcgcca cggtgtgcgg aaacccgaaa
     1381 gacattatcc gactcgaagt taccggaaac cggaacagta gcgcgcctat gcatatcgtc
     1441 gtcgacgcga cacagatcat cgagcaaaac gttgcggaag aaacaccgca atttggcggc
     1501 aacggacccg tcctcggtaa ggcgtgctac ggaacgagcc atcaggagaa tatcgcgcgg
     1561 tcttacgagg cgttcataga tggaacggaa gtcccggtaa cggccgaaga cggcatccgt
     1621 gttctccgtg tcatcgacgc tatcctcgaa agtaacggca atccggtcgc catcagctcg
     1681 tagatcagct ctcccgtacc atacaaacgg agtagcgaac gaaaaacatt tcgctactcc
     1741 gtttgcgttc ttaatcgtcg ctcccacgcg aggcgagcga ccgcgagatc gagcgccgca
     1801 ataccagtca gcacgagtat cgtcatttcg ccacataacg aagtgagaca accgagcgac
     1861 gcgtccgcga gcgttgcagg tctgccatcg acctgcgccc aacgaacgga accgatgaac
     1921 ccatcatccg cggttcggac gaagtcgccg gttcggagtg cctgctcgta agaatccacg
     1981 acgaccaatc gatcggaaaa cacgtctgaa tcggcaagaa cgacgcgcgg atcaccaccg
     2041 acaagcgtaa tctgcggaac gtccacaaca tcggctgata cgacgaccgg gttactcgac
     2101 ggtaccgccg caacgacgat atccgcgccg cgaacgcact cgcgaaccga ttcgaatgga
     2161 agaatccgca ttccaagttc cgacgacaac tcgcgcacga accgggtccg attcgacgcc
     2221 gagcgactcg ttactctcac ttcatgcacg tcgaaaacct gcgcggccgc tcgtaacgtt
     2281 gctccggcga tacgcccggt accgattatc gccaacgtca ccgactgatt ggaactcaac
     2341 caacgcgaac caagagccgc ggcgattccc gttcgtacgt tcgtaatcac ctccgcatcg
     2401 agcgtaatga cgcgattccg tgcggtatct cgaagctcga tccaagcact tctactcccc
     2461 agtacgcgcg ctccagacgt gtctcgcgca cccgactctt caataacctt cctgcagtac
     2521 cagtctggct tgccggcacg attccaaacg gcatccatct cgatgcgtaa atagtccgga
     2581 tcacccttcc ggccgagtat ttcgttacgt ggcgaaatat cgatttcacc acgcccaagt
     2641 gcttgcagcg cctcggtcac ggcgtcgatc atttgacgaa gcggtatcgc ctcaatctct
     2701 tcgtccgaga tatgaattcg ttcggtttca gtcattctct cctcgttcgg cttgcgccgg
     2761 attccactcg acggtatctc cagtaccgaa atatggacat tctctctcaa aggatacacg
     2821 cgatgaatgc aaaagaacgc gttcgtgcca cgatcaatcg acagccggtg gaccgtattc
     2881 cgctcgggtt ctactgcgcg gattgcgaca ccgtagaacg agtcatcggt cggaagacat
     2941 acgtgcgcaa caagatcggt atccaactcg cgctgtggga agggcgccgt gacgaagttg
     3001 tcgagagctt gaaagccgat tcggtcgagt tttaccagaa aatcgacacg gtagatctcc
     3061 tgacctataa agaggcacaa acactccctc cgaaagacta cgttccgaat ccgccgaaaa
     3121 agatcgcgga tgatctctgg gaagaccgca acggccgcat ttacaaagca tcgtatctgt
     3181 cgaatgaact cgtctgtgtg catgatccaa ataaacgtga cgtcgatgag tttaccccgg
     3241 agatgtttcc ggagccgtcg gaagaggacc ttgcaccgcc agacccctcg caatttgagg
     3301 cgtgggacca cttcgtcagt gtattcgcca aagatcgcta catcgcagga cattccggcg
     3361 gcatggtcgc gttgacgcac gtcggcggac aagaatccgg attgatgatg tacgtcatca
     3421 accccgaagt cgtccatgcg gcgagtcgtc gcgcggtgat aatcgccgaa gcacgcgaca
     3481 cgcaggtcat ccgtcccgag caggacggcg tgttgttcga ccaggacatg gcgggaacca
     3541 acggcccaat gatctcgccg aaactcttcc gcgagcattg ttttccgtac atgaagcaac
     3601 gcgtcgcgca tgtgaaaagt cttgggcagc agatacttct ccacaattgc ggcgataacc
     3661 gccttctcat gccgatgttc gcagaagccg gtatcgaatg ctaccagtcg ctccagacaa
     3721 acgcggggat gacactcgag aacctccagc gcgatttttc cgatcacatc gtgttctggg
     3781 gtgggctcgc cgtagaaaac ctcatcgagg gaacgccgga ggacgtgcgg cgaaacgtgc
     3841 gc
//
LOCUS       DSCC01000151            1655 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1049804, whole genome shotgun sequence.
ACCESSION   DSCC01000151 DSCC01000000
VERSION     DSCC01000151.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1049804"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..270)
                     /locus_tag="ENN56_01780"
     CDS             complement(<1..270)
                     /locus_tag="ENN56_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74249.1"
                     /translation="MRGRKNDGVSGLFAWILIAGTFIIGAIGCAESDTRESADTNLQG
                     DLIGTWFAYRDNVIENMWTFKRDGTVSNDGWPGMPSATEPALPPYH"
     gene            complement(367..1302)
                     /locus_tag="ENN56_01785"
     CDS             complement(367..1302)
                     /locus_tag="ENN56_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDS74250.1"
                     /translation="MKIAKEDHATATSKQESELGLIRTAVEANPRLLIGGHVSVAGGV
                     HRAIERGENDGFTAIQVFTRNPTQWQSPPLTDEKVDAFRTAWKHSIVQYVVAHDSYLI
                     NLATPDAALRKKSIDAMLDEIDRAEVLGIGEVVTHLGAHMGADEDAGIANLVASLNTI
                     HEQRPDSRVRIALETTAGQGTSLGYRFEHLAAVFDGVDAPERLVTCFDTCHVWVAGND
                     LAGDGGFDEVWSEFDRIVGIERLCCIHVNDAKRERGSRVDRHAHIGEGTIADDAFRRL
                     MRSDGLSSVPKIVETSEAETHHRTNVAHLVSLLHS"
     gene            complement(1299..>1655)
                     /locus_tag="ENN56_01790"
     CDS             complement(1299..>1655)
                     /locus_tag="ENN56_01790"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_927033.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDS74251.1"
                     /translation="QGSSREHAALIPRWLGVKAKIVKSFARIHLANLVNFGIVPLTFV
                     NPADYDGIDFADELELPNIREAIERGDKVITVKNVTKGTTFETTHALTERARHVILAG
                     GMLNFVKQQVKTANAK"
BASE COUNT          312 a          503 c          442 g          398 t
ORIGIN      
        1 atggtatggc gggagtgctg gctccgttgc ggacggcata ccgggccaac cgtcatttga
       61 tacggtaccg tcgcgtttga acgtccacat gttttcaatt acgttatcgc gataggcgaa
      121 ccaggttccg atgagatcgc cttgcagatt cgtatcggcg ctttctcgtg tgtcgctttc
      181 ggcgcaacct atcgcaccga tgatgaacgt accggcgatg agaatccacg cgaacagtcc
      241 cgacactccg tcgttctttc gccctctcat agtgtcccct tcccccggcc gtggccgtat
      301 gaaatcgccg cgttcgtcgc ttacgtgctc gatcgctcaa atatcgacgc ttcgacaccg
      361 aaccgcttat gaatgtaaca aacttaccag gtgcgcgaca ttggtacggt gatgcgtttc
      421 cgcttcagac gtttcgacaa ttttcggtac actcgaaagc ccgtcagatc gcatcaatcg
      481 tcgaaacgcg tcgtcggcga tcgtcccctc gccaatatgt gcgtgtcggt cgacgcgact
      541 accgcgctca cgcttcgcgt cgtttacgtg gatacagcag agccgttcga tgccaacaat
      601 tcgatcgaac tcgctccaga cctcgtcgaa gcccccatca ccggcgaggt cgttccccgc
      661 aacccatacg tggcacgtat cgaaacatgt aaccaatcgc tccggcgcgt caacaccatc
      721 gaacacggcc gcaagatgct cgaaccggta cccaagcgac gtaccctgcc cggcagtcgt
      781 ttccagcgcg atccgtacgc gggaatccgg ccgctgttcg tggatcgtgt tcaagctcgc
      841 aacgagattc gcgattcctg cgtcttcgtc ggcacccata tgcgcaccga ggtgcgtgac
      901 cacctcgccg ataccgagca cctcagctcg gtcgatctcg tcgagcatgg cgtcgatcga
      961 cttcttgcgg agtgccgcat ccggtgttgc gagattgatc aagtacgaat cgtgtgctac
     1021 gacgtactgt actatactgt gtttccacgc cgtacgaaat gcgtcgacct tttcgtcggt
     1081 caacggcggc gattgccatt gagtcggatt ccgcgtgaac acctgtatcg cggtgaaccc
     1141 gtcgttttcg ccccgttcga tcgcgcggtg cacaccgccc gcgactgata cgtgcccacc
     1201 gatgagaaga cgcggattgg cttctacggc ggtgcgaatc aggccgagtt ccgattcctg
     1261 tttcgacgta gccgtcgcgt gatcctcttt cgctattttc acttcgcgtt tgcagttttc
     1321 acttgctgtt tcacgaagtt gagcatgccg ccggcgagaa tcacgtggcg tgctcgttcg
     1381 gtcagcgcat gcgtcgtttc gaacgtcgtg ccttttgtca cgttcttgac ggtgataacc
     1441 ttgtcgccgc gctcaatcgc ttctcggata tttggtagtt cgagttcgtc ggcgaaatcg
     1501 attccgtcgt agtcggccgg attgacaaac gtcaacggaa cgatgccgaa attcacgaga
     1561 ttcgcgaggt ggattcgcgc gaacgacttc acgattttcg cttttactcc gagccaacgt
     1621 ggaatcaacg cggcatgttc gcggctggat ccctg
//
LOCUS       DSCC01000152            1587 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052746, whole genome shotgun sequence.
ACCESSION   DSCC01000152 DSCC01000000
VERSION     DSCC01000152.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052746"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..995
                     /locus_tag="ENN56_01795"
     CDS             <1..995
                     /locus_tag="ENN56_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012659621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HDS74252.1"
                     /translation="VDATEEILGIVEDLGERDLVIVLLSGGGSALLVAPADDISLKDK
                     QLTTSALLASGATIQEINSVRKHLSRVKGGQLARACGPASVMTLVLSDVIGDSLDVIA
                     SGPTVPDASTYGDALAVIDRYRLREKLPESVVERLDRGGSGDLPETPKPGDDAFARNT
                     VRIIGSNRVALEAAAAMGNANGYRSRILTSSLRGEAREVAKVLSALAEGVPDDERPCA
                     LICGGETTVTLGDDPGTGGRNQELALAAALEIDGRDDIVVLSVGTDGTDGPTDAAGGI
                     VDGESVRRARETGLDIRDALHRHDAHPLLSEIGDLVTTGPTGTNVMDVVIFLVG"
     gene            complement(1073..>1587)
                     /locus_tag="ENN56_01800"
     CDS             complement(1073..>1587)
                     /locus_tag="ENN56_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDS74253.1"
                     /translation="PGVLAMDILDNVIPYIRGEEEKVERETQKILGKLTGTTIIAADF
                     PVSATCTRVNVIDGHTETVTVGLKRAASVDDVKQAMREYGKELVAMNLPSGPKQLITV
                     MDDPFHPQPRIHRDLDGGMTTSVGRIRPDNLLQNGIKYVLLSHNTKMGAAKGATLVGE
                     ILLEKGFIKG"
BASE COUNT          322 a          439 c          482 g          344 t
ORIGIN      
        1 gcgtcgacgc tacggaagag atcctcggga tcgttgaaga cctcggtgag cgcgatctgg
       61 taattgtatt gctcagcggc ggtggttccg cgctcctcgt ggcgccggcg gacgacatat
      121 cactcaaaga caagcagtta acgacgagcg cactactcgc ttccggtgcg acgattcagg
      181 agatcaactc tgttcgcaag catctctcac gcgtaaaagg tggacaattg gcgcgtgcgt
      241 gcggtcctgc gtcggtaatg acacttgtct tgtcagacgt tattggtgac tcgctcgacg
      301 tgattgcgtc tgggccgact gttccggacg cgagcacata tggtgatgct ctcgcggtga
      361 tcgatcggta cagattgcgc gaaaaactcc ccgaatcggt tgtagaacgg ctcgatcgcg
      421 gtggatcagg agatcttccg gagacgccga aaccgggtga cgacgcattc gcgcgaaata
      481 ccgtgcgaat aatcggaagc aatcgtgttg cactcgaagc ggcggcggcg atggggaacg
      541 cgaacggata tcggtcgcga atcctcacgt cgtcgttacg cggtgaagct cgtgaagtgg
      601 cgaaggttct cagcgcgctt gcggagggtg ttcccgacga cgaacgaccg tgcgcactca
      661 tctgcggcgg cgaaaccacg gtaacactcg gcgacgatcc gggtacggga gggcggaatc
      721 aggagcttgc gcttgccgct gcgctggaaa tcgacggacg tgacgacatc gttgtgttga
      781 gcgttggtac ggatggaacc gatgggccaa ccgacgcagc gggtggcatc gtggatgggg
      841 aatccgtgcg tcgagcgcgt gaaacggggc tggatatccg cgatgctcta caccgtcatg
      901 acgcgcaccc gctgctctcc gaaatcggcg atctcgtcac taccggtcct accggcacaa
      961 acgtcatgga tgtcgtgatc tttctcgtcg gatagctgac gacattgccg tacagaagaa
     1021 cgcgggagcc cggtataccg aactcccgcg ctttttctat tcgcaaatag actcagcctt
     1081 tgatgaaacc cttctcgagg agtatttcac caaccaacgt cgcgcctttc gcagcgccca
     1141 tcttcgtgtt gtggctgagc aacacgtact tgatgccgtt ctgtaggagg ttgtcaggac
     1201 gaatacgtcc gaccgaggtc gtcattccgc cgtcgaggtc gcgatgaatc cgcggttgcg
     1261 ggtggaacgg gtcgtccatc acggtaatca actgcttcgg tcccgacggc aagttcatcg
     1321 caacgagttc cttgccgtat tcacgcatcg cctgctttac gtcatcgacg ctcgcggcac
     1381 gcttcaatcc aacggtcacg gtttccgtat gcccgtcgat tacgttcaca cgcgtgcagg
     1441 ttgcagatac gggaaaatcg gcggcgatga tggtcgtgcc ggtcaacttg ccaagaatct
     1501 tctgtgtttc gcgctccact ttctcttctt caccacggat gtacgggatg acgttgtcga
     1561 ggatgtccat tgcgagaaca ccaggac
//
LOCUS       DSCC01000153            1827 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043611, whole genome shotgun sequence.
ACCESSION   DSCC01000153 DSCC01000000
VERSION     DSCC01000153.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043611"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..813
                     /locus_tag="ENN56_01805"
     CDS             <1..813
                     /locus_tag="ENN56_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74254.1"
                     /translation="VTDQAEIAYSGAIRLIGGKGFIWLAIIIGAVFLDPNVIAGFPSL
                     QEIWHVPFGIRELIMFAVMFLAYKTANKDALKGNDFNFEPIREVGFLFIGIFMTMVPA
                     LQLIAYEAQHYASVLTPGLFYFGTGGLSAVLDNTPTYINFFVASMGKYGLEAGNVSHV
                     RLFTACPETILYLEAISVASVFFGALTYIGNGPNFMVKSISDQNGLPMPSFFGYLVKW
                     ALPILLPIFIIVWALFYRNPVDHGDALKVEPCFQIEQIMQSAGMTSGMPAGH"
     gene            926..1336
                     /locus_tag="ENN56_01810"
     CDS             926..1336
                     /locus_tag="ENN56_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74255.1"
                     /translation="MSQADPGTSPRTIQQALDANLKGFTIGRRLSIPFRCELISILVG
                     KHRSFVGTPDIITDFSPSQREISVVERPNRMDLYFDKYESLQEELGHWKGTLIVTCCE
                     VGDDIFEPNDRRRIEFVFRDDTSEVTIQEFREEA"
     gene            1344..>1827
                     /locus_tag="ENN56_01815"
     CDS             1344..>1827
                     /locus_tag="ENN56_01815"
                     /inference="COORDINATES: protein motif:HMM:PF04015.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2088 domain-containing protein"
                     /protein_id="HDS74256.1"
                     /translation="MNWPRTIRVEQTFDHPTISDPVSAARQSLSGVLDGVGVALDGKS
                     VAITVGSRGIANLPAIIRALVDELTQRGARPFIVPSMGSHGGGTASGQTGVLHALGVT
                     EEAVGAPIRSSMETVEVGRTEDGFPVVIDRLASEADHILVVNRVKPHTEFFGPYQSGL
                     M"
BASE COUNT          376 a          520 c          532 g          399 t
ORIGIN      
        1 gttaccgatc aggccgaaat cgcctattca ggcgcgattc gccttattgg cggaaaaggt
       61 ttcatctggc tcgcgatcat catcggcgcc gtcttcctcg atccgaacgt gatcgccgga
      121 ttcccgtcac tgcaggagat atggcacgtt ccgttcggca tccgcgagtt gattatgttc
      181 gcggtgatgt tccttgcgta taagaccgcg aacaaggacg cgctcaaggg caatgatttc
      241 aacttcgagc caattcgcga agtcggattc ctcttcatcg gcatcttcat gaccatggtg
      301 ccggcgctgc agttgatcgc gtacgaagcg cagcattacg cttcggtgct cacgccgggc
      361 ttgttctact ttggaaccgg tggcttgagt gcagtgctcg acaatacgcc gacgtacatc
      421 aacttcttcg tggcatccat gggtaaatac ggccttgaag cgggcaatgt gagccatgtg
      481 cgtctgttca cagcgtgccc ggagacgatt ctgtatctcg aagcgatctc ggttgcgtca
      541 gtgttcttcg gtgcactgac ctatatcggc aacggtccga acttcatggt gaaatcgatc
      601 agcgaccaga atggtttgcc catgccgagc ttcttcggat acctcgtgaa gtgggcgctg
      661 ccgattctgc taccgatctt catcattgtt tgggcgctgt tctatcggaa tccggtcgac
      721 catggcgacg cgctgaaggt cgagccgtgc ttccagatcg agcagatcat gcagtccgcc
      781 ggaatgacat cgggtatgcc ggccggacac tgatcggctc gtaggaaatg ccgcgcgcgg
      841 gatgtcgaaa cgtacggcat cccgcgatgc gttctgtaca tgagcgggta cctctcaaac
      901 gcaagagcga agaaagggga cgatcatgtc acaagcagac ccgggaacgt cgccgcgcac
      961 gattcaacag gcgctcgacg ccaatctcaa gggattcacc atcgggcggc gtctgagtat
     1021 cccgttccgc tgcgagctca ttagcatcct cgtcggcaag caccgctcct tcgtcggcac
     1081 tcccgacatc atcacggatt tctccccgtc gcagcgtgag atttccgttg tcgagcgtcc
     1141 gaaccgtatg gacctgtatt tcgacaagta cgagtcgcta caggaagagc tcggtcactg
     1201 gaaagggacg cttatcgtca cgtgctgcga ggtcggtgac gacatcttcg aaccgaacga
     1261 tcgtcgtcga atcgagttcg tcttccgcga cgatacctcc gaagtgacca tacaggagtt
     1321 tcgagaagaa gcgtgaggcg tgaatgaact ggccgcggac aatacgagtc gagcaaacgt
     1381 tcgatcaccc gacgatcagc gatccggtaa gcgcggcgcg acagtcactc tccggtgttc
     1441 tcgacggcgt cggagttgcg cttgatggga aaagcgtcgc gatcacggta gggagtcgtg
     1501 gcattgcgaa tctacctgcc atcatccgag cactcgtcga cgaactgacg cagcgtggcg
     1561 ctcgaccgtt catcgttccg tcgatgggga gtcacggtgg cggtaccgcg tcgggacaga
     1621 ccggcgtact ccatgcactc ggcgtcaccg aagaggccgt aggcgcaccg atccgttcgt
     1681 cgatggagac agtcgaggtt ggccgtacgg aggacggttt tcccgttgta atcgaccgtc
     1741 tcgcatccga agctgatcac atcctcgtcg taaaccgcgt caagccacac acggagtttt
     1801 tcggacccta ccagagcggt ctgatga
//
LOCUS       DSCC01000154            2173 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034406, whole genome shotgun sequence.
ACCESSION   DSCC01000154 DSCC01000000
VERSION     DSCC01000154.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034406"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(198..>2173)
                     /locus_tag="ENN56_01820"
     CDS             complement(198..>2173)
                     /locus_tag="ENN56_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDS74257.1"
                     /translation="REKEIERVSQVLSRRKKNNPVLIGEPGVGKTAIAEGLAQKIEAK
                     EVPHVLQNKRIVTLDMGGIVAGTKYRGQFEERVKAIMNELVQNREVIIFIDELHTIVG
                     AGSAEGTLDASNLFKPALSRGELQCIGATTLDEYRKYIEKDGALERRFQTIMVEPPSV
                     SDTERILLGLRGKYEEHHRVEYPDATIKAAVTMAERYLHDRYQPDKSLDVIDEAGSRV
                     RLSHVSVPREVKDLEDQIADVEKQKERAVNEQNFEEAANLRDTERRLRDKLAVERKNW
                     EQARESDVVEVTEDDIAYVVSSMTGIPVFRLATDESEKLLHIEDQLRNRVVGQEGAIN
                     AVSRAIRRSRSGLKDPDRPIGSFIFLGPTGVGKTELGKALAAHLFDDEDAAIIIDMSE
                     FMEKFAVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEKAHPDVFNILLQVL
                     DDGRLTDSYGRKVDFRNTILIMTSNLGSRDIGDISGFGFTSRDGLPSRSEMETAINRE
                     MKRVFSPEFLNRVDEVVIFNTLTHENMLRITDIVIDDVRKRIVERDLKMTLSHDAKDL
                     LAEKGFDAKLGARPLKRTIQRLLEDPLSEEILGGKFADGSTILVDRDGDKLSFSLVDV
                     SGDNGGSDDGGDDGGPGVSEPEGPVHVDAEAHS"
BASE COUNT          471 a          663 c          527 g          512 t
ORIGIN      
        1 gccaaaccgt atacgagaaa cgttgagaga aaataacgat acgcagattg ataccgggta
       61 acgaggggct cgcgaactgc ttcgtaagta ctaacgtgag gatgaacaaa aagtgtatcg
      121 gttttccgat taccacacgg agagtcccgc ctccgcgtcg gagacgggac tcgtgttggt
      181 catcgctcgc tctcttacta cgagtgagcc tcggcgtcta cgtgtactgg gccttccggt
      241 tctgatactc cgggaccgcc gtcatcaccg ccgtcgtcgc tcccgccgtt atcacccgat
      301 acgtcgacga gtgagaaact cagtttatca ccatcacgat ccacaaggat cgtcgagccg
      361 tccgcgaact taccaccgag gatttcctcg ctgagcggat cttcgagtaa gcgttgaatc
      421 gttcgcttca aaggccgcgc accgagcttc gcgtcgaatc ccttttccgc gagaaggtcc
      481 ttcgcgtcgt gcgaaagagt cattttcagg tcgcgctcga caatccgctt tctcacgtcg
      541 tcgataacga tgtcggtaat cctcagcatg ttctcatggg tgagtgtatt gaaaatcacc
      601 acttcgtcaa ctcggttgag aaactccggc gagaaaaccc gcttcatctc acggttgatt
      661 gccgtttcca tctccgaacg agaggggagc ccatcgcgtg acgtgaagcc aaatcccgat
      721 atatcgccga tatcgcgact tccgaggttc gatgtcatga tgagaatcgt attccgaaaa
      781 tcgaccttcc ggccatagct atccgtcagg cgcccgtcat cgagcacctg aagaaggata
      841 ttgaatacat ccgggtgcgc tttctcgatc tcatcgagga gaatcaccga atacggcttc
      901 cgacggacct tctccgtgag ctgaccaccc tcttcatacc caacatatcc cggaggcgcg
      961 ccaacaagcc ggctcacggc aaatttctcc atgaactccg acatgtcgat gatgatcgcc
     1021 gcgtcctcgt catcgaaaag atgcgcggca agcgctttcc caagctcggt tttcccaaca
     1081 ccagtcggtc caaggaaaat gaacgacccg atcggacgat ccggatcttt cagaccgcta
     1141 cgcgaccggc gaatcgctcg ggatacggca ttgatcgcac cttcctgccc aacgacgcga
     1201 ttccgtagtt gatcttcgat atggagaagc ttttcagact catccgtcgc gaggcgaaag
     1261 accggaatac ccgtcatgct gctcacaaca tacgcgatat cgtcttcggt cacttcgact
     1321 acatcgctct cacgagcctg ctcccagttt ttccgttcga ctgcaagctt gtcgcgaagg
     1381 cggcgttctg tgtcgcgaag attcgccgct tcttcaaagt tttgttcgtt gacggcacgc
     1441 tctttctgct tttcgacgtc cgctatctga tcctcgagat ccttaacctc acgcggcacc
     1501 gaaacatgac tcaagcgcac ccgagagccg gcttcgtcaa ttacatcgag cgatttgtcc
     1561 ggttgatacc gatcgtgcag gtatcgttcc gccatggtca cagcggcttt gatcgttgca
     1621 tccggatact ccacgcgatg gtgttcctcg tacttgccgc gcagaccgag caggatccgc
     1681 tccgtgtccg aaaccgaagg cggctcaacc atgatcgtct ggaaacgacg ctccaacgcg
     1741 ccatcctttt cgatgtactt gcgatactcg tcgagcgtcg tcgcaccgat acactggagt
     1801 tcgcctcggg acaatgccgg tttgaacaag ttcgaggcat caagcgtgcc ttcagccgat
     1861 cccgcaccga cgatcgtatg caactcatcg ataaagatga tcacttcacg attctgcacc
     1921 aactcattca tgatcgcttt gacgcgctct tcgaactggc cacggtattt cgtgccggca
     1981 acgataccgc ccatgtccag cgtcacaatg cgcttattct ggagcacgtg cggaacttct
     2041 ttcgcttcga tcttttgcgc cagtccttcc gcaattgcgg tcttaccaac acccggctcg
     2101 ccaatgagta ccggattgtt ctttttccgc cgcgaaagca cctgactgac acgctcgatc
     2161 tctttctctc gac
//
LOCUS       DSCC01000155            3304 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1019635, whole genome shotgun sequence.
ACCESSION   DSCC01000155 DSCC01000000
VERSION     DSCC01000155.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1019635"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            376..903
                     /locus_tag="ENN56_01825"
     CDS             376..903
                     /locus_tag="ENN56_01825"
                     /inference="COORDINATES: protein motif:HMM:PF00027.27"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDS74258.1"
                     /translation="MAPNAVWSSIKSLVSAPATLDEAFRKLPMFEKLNRKQMKEVERI
                     IHRRTYRAGEEIFHAGDTGVALYVVLAGEVRIVLPGEEPEEGIEVARLSAGDFFGELA
                     LLDSSPRSASAIAATPTEAAALARPDWLDLVDRQPAIGVEMLLPLAKMLAVRLRAANR
                     TTVEELRTGKASKEK"
     gene            900..1982
                     /locus_tag="ENN56_01830"
     CDS             900..1982
                     /locus_tag="ENN56_01830"
                     /inference="COORDINATES: protein motif:HMM:PF01594.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDS74259.1"
                     /translation="MSLKTYDERGAWWPVLLTATVSFAALYMVRSTLPPVIFAGIMAY
                     LLNPFVDFGERRALPRWAAIGLIYLIIIGLVILSVFYLVPALINQLTDIQRELRSLWP
                     LVPQYLDVWVDWVRVRFPSAVSMIVGDEPLGVRVVSGAQTWITSAIAQAPTMITSVLT
                     NIASFLLYFVMVPFIAFFLLRDGRAFKMWLIQLVPNRYFETTLHIVGGIGESVGWYLR
                     GLLIQATILATIASIGLSIVGVKYAVIIGIVAGIANMIPYVGPTLGVIIGLMIVVTTG
                     EGSILGVLIVFGIAQFIDNWFIAPFVMSRSVKLHPLIVFLAVILGGTYAGILGMFLAI
                     PVTGAVFVTFKRLRDGLKPPVYAMEP"
     gene            2130..>3304
                     /locus_tag="ENN56_01835"
     CDS             2130..>3304
                     /locus_tag="ENN56_01835"
                     /inference="COORDINATES: protein motif:HMM:PF13715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74260.1"
                     /translation="MHTYKLTLHSILIVVFSIVAFSGWVNAATTGIISGTVTDADGSP
                     LPGAAVMIDGTRLGASTDSDGQYVVLLVPPGVYTVSSQLIGYNPTSVTDVRVSADLTS
                     RLNFRLEQEAIEVDAIVVTARRDPIRIDVTSSQTIVDAARVSEMPVSQMLGVLNYQPG
                     VNVVRGNELEIRGGGPSEIRFQVDGVDRTDGMTGKGYTQLNQVLVSEVTLLTGGFNAE
                     YGNVRSGMVNVVVKEGNERGTLMPWIAAVASYAPAQKKHFGPGAYDENQYDYWRLLKT
                     DSAATGGPIYWPDLYEETRTDTAFMSVVKGSRAQYRVHQGWNDVVKTANSSGLRYGPP
                     YSHNGWTLDDIKEAWAWETNMNEEVWQYSHEPDVTADVALGWALPRKLGGVVIGYSY"
BASE COUNT          690 a          819 c          982 g          813 t
ORIGIN      
        1 atgagggagc gcatgtagac gtcggtccgc gaccgggtga gggaattgaa tccgaacacg
       61 tcggtagcga tattcaacct gtgtttttaa tcgaggggga acttaccggg cggaagttcc
      121 acgaagccgc actgacagag gtacccgata tccctgctcg aacgcaggtg caactcgacc
      181 tcgttgttgc gccaaacggt tcagttgtca gcgtgattgt cgttcggaaa gaaaacgcgt
      241 tgttggaaag gttcgcggtg gattacgtgc ggcgctcacg attcgattcg ttgtcgccgg
      301 aggctccgca agagaatcag acgggccgta tgacgatcac atttgctcag cggggagaat
      361 aggagtcgga cgccgatggc accgaacgca gtctggtcca gcatcaagtc gctggtgagc
      421 gcgccggcta ctctcgatga agcttttcga aagctcccga tgttcgaaaa gctcaatcgg
      481 aagcagatga aagaggttga gcgaatcatc catcggcgta cgtatcgtgc tggtgaggag
      541 atattccatg ctggcgatac gggcgtcgcg ctttacgttg ttttggctgg tgaagttcga
      601 atcgttctcc cgggagagga gccggaagaa ggcattgaag ttgcgaggtt gagtgccggt
      661 gatttcttcg gggaacttgc gctgcttgac tcgtcaccac gttcggcatc tgccattgcg
      721 gcaacaccga ctgaagcggc ggcgctggcg cgtccggact ggttagattt ggtcgatcga
      781 cagccggcta tcggtgtcga gatgcttctt ccacttgcga aaatgcttgc ggtacgcctt
      841 cgcgcggcga acaggacaac tgtggaagag cttcgtaccg ggaaagcgtc gaaagaaaaa
      901 tgagtctcaa gacgtatgat gaacggggtg cgtggtggcc ggtattgctt accgcgacag
      961 tttcgttcgc cgcgttgtac atggttcgtt cgacgttgcc gccggtgata ttcgccggca
     1021 tcatggcgta cctgctgaat ccgtttgttg actttgggga gcgacgggcg ctgccgcgat
     1081 gggcggcgat tgggttgatc tacctcatta ttatcgggtt ggtcatactt tcggtcttct
     1141 atcttgtccc tgcgcttatc aaccaactga ccgatatcca acgggaacta cggtcactgt
     1201 ggccactggt cccgcagtat ctcgatgtct gggttgactg ggtacgagtt cgatttcctt
     1261 cggctgtttc gatgattgtt ggtgacgagc cgttaggggt tcgtgtggta tccggagcgc
     1321 aaacgtggat aaccagcgca atcgctcagg caccgacaat gatcacaagt gtgctgacca
     1381 acatcgcgag ctttctcctc tactttgtga tggtaccgtt tatcgcgttt ttccttctgc
     1441 gtgacggtcg agcgttcaag atgtggctga tccaactcgt gccaaatcgg tatttcgaaa
     1501 cgacgctgca catcgtcggt ggtatcggtg aatcggtcgg ttggtatctc cgcggactac
     1561 tgattcaagc gacgatactc gcgacgatcg catcgatagg actttcgatc gtcggtgtca
     1621 agtacgcagt cattatcggt atcgtcgccg gtatcgcgaa tatgattccg tacgttggac
     1681 cgacgctcgg cgtcatcatc ggattgatga tcgtcgtgac gaccggcgaa ggatctattc
     1741 tcggtgttct tatcgtattt gggatcgcgc aattcatcga caactggttc atcgcgcctt
     1801 ttgttatgtc tcgaagcgtg aaacttcatc cgctcatcgt gtttcttgcg gttattcttg
     1861 gtggcacgta cgccggcatt ctcggaatgt ttctcgcaat accggtcacc ggtgccgtat
     1921 tcgtcacgtt taaacggttg cgcgacggtc taaaaccacc ggtctacgcg atggaacctt
     1981 gatgcgattt tcctacacgc acctgcgttg ccccaagcgg atcatccgtt gtaccatact
     2041 accaagctcc gcgtaacctt ataccgttca cgccttaatc ctcaagtgaa gccaccgctt
     2101 cgaattctga acccagccgg gggaagcgca tgcatacgta caaattgaca ctgcactcga
     2161 tcctcatcgt tgtattcagt atcgttgcgt tttctgggtg ggttaacgcg gctacaacgg
     2221 ggattatctc cggaaccgta acagatgccg acggttcgcc gcttcccggc gccgcagtga
     2281 tgatcgacgg tacgcgactc ggagcttcta ccgattcgga tgggcagtat gttgtcctct
     2341 tagtgcctcc cggcgtttac acggtttctt cgcagcttat cgggtacaac ccgacgagtg
     2401 ttacagatgt tcgagtcagc gccgatctca cgtcccgatt gaactttcga ctggagcagg
     2461 aggcaatcga ggttgacgcg atcgttgtga cggcgcggcg agatcccatc cgtatcgatg
     2521 tgacaagttc acagacgatc gtagatgctg cccgtgtctc ggaaatgcca gtcagtcaga
     2581 tgctcggcgt gttgaattac cagccaggcg tgaatgtcgt tcgcgggaat gagttggaga
     2641 ttcgtggcgg cggaccttcg gaaattcgtt ttcaggtgga tggtgttgat cgcaccgatg
     2701 gtatgacggg gaaggggtac acacagctta atcaggttct cgtaagcgaa gtgacgctcc
     2761 taaccggtgg ctttaacgcc gagtatggga atgtacgttc cggtatggtc aacgttgtgg
     2821 tgaaggaagg gaacgaacgc ggcacgttga tgccgtggat agcagcggtt gcgtcatatg
     2881 ctcccgcaca aaaaaagcat tttggacctg gcgcctacga cgaaaatcaa tacgactact
     2941 ggcgcttgtt aaagactgat tcggcagcaa caggcggccc gatctactgg ccggatttgt
     3001 acgaggaaac gcgtactgat actgctttta tgagcgtagt gaaaggctct cgagcccagt
     3061 accgcgtcca tcagggatgg aacgatgtgg tcaagacggc gaactcgtcg ggactgcgtt
     3121 acggtccgcc gtattctcac aacggctgga cattggacga tatcaaagag gcgtgggcgt
     3181 gggaaacgaa catgaacgaa gaggtttggc agtattccca cgagccagat gtcacagcgg
     3241 atgttgcgct tggctgggca ctgccgcgga agcttggtgg cgtcgttatt ggatatagct
     3301 acag
//
LOCUS       DSCC01000156            5492 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1009840, whole genome shotgun sequence.
ACCESSION   DSCC01000156 DSCC01000000
VERSION     DSCC01000156.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1009840"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..468)
                     /locus_tag="ENN56_01840"
     CDS             complement(<1..468)
                     /locus_tag="ENN56_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74261.1"
                     /translation="MISYEGRNRRTVNYLKSMYFDYPEWIETNVSIMMATWLKYRDEA
                     EDIVLRHPRVFPGWKKGKLGDYSFPDASPLYLSGYHRDKWGTLWHNIERGLDSIPVEY
                     PLDDWGKWDSWRTPDPETETEWGLRDWDAVEREIAETKRNGGLARGGGLMHGFF"
     gene            692..1753
                     /locus_tag="ENN56_01845"
     CDS             692..1753
                     /locus_tag="ENN56_01845"
                     /inference="COORDINATES: protein motif:HMM:PF13641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS74262.1"
                     /translation="MNGNTQTDSKLPVVSVIMPIRNEGTFIEQSLGAVLDQDYPQDLL
                     EVLVVDGMSDDGTRETVRSILEHTDISGQLLDNPHRIVPTAMNIGIQAAKGSIIVRVD
                     GHTIIETDYVRRAVEALRRTGAHNVGGLMRPVPSGYVPRAVAAAMSSPFGVGASIFHY
                     AEREAEADTVYLGVYPKWVLEHVGMYDEEFVRNQDDELNDRVRKAGGRIMLCPELKSA
                     YYPRSTLKKLAKQYFQYGWWKVRCYQKHPKHVGKGHLAPTAFVVALISGLALSTLNRI
                     VGLLWLTMMALYLIGATASAIRLGPKYGWSLVPIMPIVFFTIHVSYGLGMLLGIIRFA
                     KRWRDDACPLSPLPPHTPL"
     gene            1793..2959
                     /locus_tag="ENN56_01850"
     CDS             1793..2959
                     /locus_tag="ENN56_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS74263.1"
                     /translation="MSKKADFIAFHRPLIEADDIQAVLDSLKTGWITTGPRVAEFEEA
                     FERLTGTTCAVGVNSCTAAMHLAVAALGLGPGDEVITTPYTFVATSEAILYTGAKPVF
                     VDVKPTDANIDPSLIEAAITPKTKGIMPVHIAGFPVEMDRVMEIARAHGLWVVEDAAH
                     GIETQWKGVRAGAWGDAGAFSFYATKNITTAEGGMLTTNDENLADRARHLSLHGMSRD
                     AWKRYTATGSWYYEITSQGYKYNMPDVLAALGLSQLKKIDVWHGIRQKYAERYSQAFA
                     SNPALQPPSVPNDGVVAWHLYPLRLNLDALRISRGDFIEQLRKQGVGTSVHFIPLNLQ
                     PYYQETLGYRAGQFPVAEAAYEGEISLPLYPGLRESEIDRVIESVITVAGESLA"
     gene            2987..3637
                     /locus_tag="ENN56_01855"
     CDS             2987..3637
                     /locus_tag="ENN56_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDS74264.1"
                     /translation="MLFDQIVAFIGITALSPVFVVFAILVRLTSSGPAFYVQERIGRN
                     AKPFRLLKFRTMTVAKPSTDTRQITVAGDARITPLGHFLRRYKLDELPQLINVLVRHM
                     RLVGPRPEVAKYVAYYRGEQHRVLSVHPGITDIATLYYRNESDELAAADDPETYYVET
                     VLPRKLHLNLLYIDSRTFLSDIRVILLTALVSISPTSGARYVRNWIEKRYLADISM"
     gene            3702..3929
                     /locus_tag="ENN56_01860"
     CDS             3702..3929
                     /locus_tag="ENN56_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74265.1"
                     /translation="MAILLWRVLMVIVAVALLFAVIWVITRSVNGTRRQKRTIRRLDQ
                     ETDPDIADAEYRDVGEGDAGKKRDSDNTHGE"
     gene            3856..4959
                     /gene="holA"
                     /locus_tag="ENN56_01865"
     CDS             3856..4959
                     /gene="holA"
                     /locus_tag="ENN56_01865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDS74266.1"
                     /translation="MRNTETSVKETPVRNEIRTTRMANKPRRARGSSSAEPANPDQVV
                     KSIVEAGSGAYLLLGENGLDLEEVVDRVIESLTTAETRAFNFDEFDASDRETTGEVVS
                     SAVRSFPMLADIRVVVIRTMHDANDSVGSELAHLVDGTIEGTALLMIGEKLDGRKRWA
                     QTVAKHATRFDFGIPKGRAFPAWLKRRAERQGATLSDDAATMLIEFTAQDVWRVASEL
                     EKVVLYALPRTEITVDDVEASVGLTREDTVYSLTDRIAERNVDAALGIAHRMAQADAH
                     PAYLVGMIVRHWQTLRLASDLAASSRQKELSALLGENRNWILNKYLTQARTLRREHVR
                     SGFRLALAAESAIKAGWEPTVVLDGLICQLAAR"
     gene            5055..5378
                     /gene="trxA"
                     /locus_tag="ENN56_01870"
     CDS             5055..5378
                     /gene="trxA"
                     /locus_tag="ENN56_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDS74267.1"
                     /translation="MAKPIVVTDDTFEQEVLKSDIPVVVDFWAVWCGPCRMIAPVVEE
                     LAVQYDGKVKFAKVDVDDQQKYAGQFGIRSIPTLLIFKDGKVVDQIIGAVPKGELDKK
                     VKAQL"
     gene            5391..5466
                     /locus_tag="ENN56_01875"
     tRNA            5391..5466
                     /locus_tag="ENN56_01875"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:5424..5426,aa:Thr,seq:cgt)
BASE COUNT         1239 a         1468 c         1551 g         1234 t
ORIGIN      
        1 gaaaaaaccg tgcataaggc caccgccgcg agcgaggcca ccattccgtt tcgtctccgc
       61 gatttcccgt tcgacggcat cccaatcgcg cagaccccat tcggtctcgg tttccgggtc
      121 cggcgtcctc catgaatccc atttccccca atcatcgagc ggatattcaa cgggaatgct
      181 gtcaagaccg cgttcgatgt tgtgccagag tgtcccccac ttgtcgcgat ggtagccgga
      241 aaggtagagt gggctcgcat cggggaagga atagtcgccg agttttccct ttttccatcc
      301 ggggaatacg cgcgggtgtc gcaatacgat atcttccgct tcgtcgcggt atttcaacca
      361 cgtggccatc ataatactga cgtttgtttc gatccattcc ggataatcga aatacattga
      421 tttgagatag ttcactgtgc gacggttgcg tccttcatac gaaatcatgg cgttgctccg
      481 agtgtggaac ggttcgagat gtcatgatta caactcacgc gaggcgataa cacgctatcg
      541 catgtgcata ccgatgtaag gtatcatgaa acggaaatgg gggaagggcg acggctccgg
      601 attgacggcc gcactcgcgt gccgttgttt tactgtggcg ggtggtggat atggcgggta
      661 ggtcgcgcgt tttttcgata cgagtggtga catgaacgga aacacacaga ccgactccaa
      721 acttcccgtc gtcagcgtca ttatgcccat tcgcaacgaa ggtacgttca tcgagcagtc
      781 gcttggcgcc gtcttggacc aagattaccc tcaagacctt ctcgaagttc tcgtcgtcga
      841 tggtatgtcc gacgacggca cccgcgaaac ggtacgctcc attctcgaac acaccgatat
      901 ttccggtcaa ctcctcgaca atccccaccg aatcgttcca accgcgatga acatcggtat
      961 tcaagcggcg aaaggtagca ttatcgtccg tgtagacggg cacacaatca ttgaaacgga
     1021 ttacgttcgc cgcgcagtgg aggcgctgcg ccgtaccggc gctcacaatg tcggtggctt
     1081 gatgcgacct gtaccttcgg gctatgtccc gcgtgcagtc gccgcggcaa tgtcatcgcc
     1141 gttcggtgtt ggtgcgtcca tcttccacta cgccgaacgc gaggcggaag cagacacggt
     1201 gtatctgggg gtgtatccga aatgggtact cgagcatgtc gggatgtatg atgaagagtt
     1261 cgtgcgcaat caagatgacg aactcaacga ccgtgtaaga aaagccggcg gccgcatcat
     1321 gctgtgcccg gaactcaaat cggcctatta tccacgatcg acgttgaaaa aactggcgaa
     1381 acagtacttc caatacggtt ggtggaaggt gcgctgctac cagaagcacc cgaagcacgt
     1441 ggggaaaggg catcttgctc ctacggcgtt cgtagtcgct cttatttcgg ggcttgcgct
     1501 gtcaacactg aaccgtatcg tcggcttgct ctggctgacg atgatggcgt tgtacctcat
     1561 cggagctacg gcttcggcga ttcgccttgg gccgaaatac ggatggagcc tcgttccgat
     1621 tatgccaatc gtgtttttta ccattcacgt cagttacggc ttaggcatgc tactcggtat
     1681 aattcgtttc gcgaaacgat ggcgggatga cgcgtgccca ctgtcaccac tcccaccgca
     1741 cacgccgctg tgattcgatc gccaaacgaa ctcggagccc acacaaaaaa atatgtctaa
     1801 aaaagctgac tttatcgcct ttcatcgtcc gcttatcgag gcagacgaca tacaggccgt
     1861 cttggattcg ctcaagactg gttggataac caccggacct cgtgttgcag agttcgaaga
     1921 agcgtttgag cgacttaccg gtacgacgtg tgccgtcggc gtcaattcct gcaccgcagc
     1981 aatgcacctc gctgttgccg cgctcggact cggaccgggc gatgaagtca taacgacacc
     2041 atacacgttc gttgcgacga gcgaagcgat cctctacacc ggagcgaagc cggtattcgt
     2101 agatgtcaaa ccgaccgatg cgaacatcga tcccagtctg attgaggcgg cgattactcc
     2161 aaagacgaaa gggatcatgc cggttcatat tgccggcttt ccggttgaga tggaccgcgt
     2221 aatggagatc gcgcgtgcgc acggtctgtg ggtggttgaa gacgccgcgc acggaattga
     2281 aacgcagtgg aaaggtgttc gcgcgggtgc gtggggcgat gcgggtgcat tcagcttcta
     2341 tgctacgaag aatatcacaa cagcggaagg tggaatgctg acaacgaacg acgagaatct
     2401 cgccgatcgc gcgaggcatc tttcgttaca cggcatgagt cgcgacgcgt ggaaacggta
     2461 cacggcaacc ggttcgtggt attacgagat cacgtcgcaa gggtacaaat acaacatgcc
     2521 ggacgttctc gctgcactcg gtctcagtca actcaaaaag atcgacgtct ggcacggtat
     2581 ccgacagaaa tacgccgaac ggtattccca agcgttcgca tcgaatccgg ccctccagcc
     2641 gccaagcgtg ccgaacgatg gcgttgtcgc gtggcatttg tatccactcc gtctgaatct
     2701 cgacgcgttg cgaatctcac gcggcgactt catcgaacag ctgcgtaagc aaggagtcgg
     2761 cacaagcgta catttcatcc cgctcaatct ccagccgtac tatcaggaga cactcggcta
     2821 ccgtgcggga cagttcccgg ttgcggaagc ggcgtatgaa ggtgaaatct cactcccgct
     2881 ctatccgggt ctccgcgaat ccgaaattga tcgcgtgatc gaatcggtta tcaccgttgc
     2941 gggcgaatcg cttgcgtaag cgtgggcgga aggattccac acgagaatgc tctttgatca
     3001 gatagtcgca ttcataggca ttacggccct ttcgccagta ttcgtggtat tcgcgattct
     3061 cgttcgtctg acatcgagtg gacctgcgtt ctacgtacaa gaacgcatcg gtcggaacgc
     3121 aaaaccgttt cgccttctga aattccgaac aatgaccgtg gcaaaaccaa gtacggatac
     3181 gcgacagatc accgtcgccg gcgatgctcg cattacacct cttggacatt ttctacgacg
     3241 ttacaagctc gatgaactac cgcaacttat caacgtgctc gttcggcaca tgcgtctcgt
     3301 cggtccgcgt ccggaagtgg cgaaatatgt tgcttattac cgaggagagc agcatcgcgt
     3361 attgtccgtt catccgggta tcaccgacat cgcaacgctg tattaccgca acgaatccga
     3421 cgagctcgcc gcggcggatg atccggaaac gtattacgtc gaaacggttc tgcctcgaaa
     3481 actgcatcta aatctgcttt acatcgacag ccgcacgttt ctttcggata ttcgagttat
     3541 cttgctgact gcgcttgttt ccatctcgcc gacatcaggt gcgcgttacg tacgaaactg
     3601 gattgagaaa cggtatcttg ctgatatatc gatgtaacca gatcacgaaa aacgagaagc
     3661 gaatagtgga aacacactga cgaaaggttc ttctccttcc catggcgatt cttctttggc
     3721 gtgtgttgat ggttatcgtt gcggttgcgc tactcttcgc ggtaatctgg gtgattaccc
     3781 ggtccgtcaa cggtacgcgc cggcagaaac ggacgatccg gcgcctcgat caggaaaccg
     3841 atccggatat cgcggatgcg gaataccgag acgtcggtga aggagacgcc ggtaagaaac
     3901 gagattcgga caacacgcat ggcgaataaa ccacgtcgcg cgcgcggttc ctcatccgcc
     3961 gaacccgcaa atcccgatca ggtcgtgaaa tcgatcgtcg aggcggggtc aggtgcgtat
     4021 ctccttctcg gcgagaacgg cctcgatctg gaggaagtag tcgaccgggt aatcgagtcg
     4081 ttgacaacag ccgaaacgcg ggcattcaac tttgatgaat tcgacgcgtc ggaccgagag
     4141 accactggag aagtggtttc ttcagcggtt cgttcgtttc ccatgctcgc cgatatccgt
     4201 gttgtcgtta tccgtaccat gcacgacgcg aacgatagtg tcggatccga gctcgctcat
     4261 ttggttgatg ggacgatcga aggcaccgcg ctcctgatga tcggcgagaa gcttgacggt
     4321 cggaagaggt gggcgcaaac tgtcgcgaaa cacgctaccc ggtttgattt tggaattccg
     4381 aagggacggg cgtttccggc gtggctcaaa cgccgagccg agcgacaggg agcaacgctt
     4441 tccgacgacg cggcgacgat gctcatagag tttaccgcgc aggatgtctg gcgtgtcgca
     4501 agtgaactcg agaaggttgt tctttacgca ttgccgcgaa cggagattac ggtagatgat
     4561 gtcgaggcat ccgtcgggtt gacgcgcgaa gacacggtgt attcgctgac ggatcgtatc
     4621 gcggagcgaa acgtggatgc ggcacttggc atcgctcatc gcatggcgca agccgacgcg
     4681 catccggcgt atcttgttgg gatgatcgtc aggcactggc agacgcttcg gttggcatcg
     4741 gatctcgcgg ccagctcccg acaaaaagag ttgtcggcgt tgctcggaga gaatcggaat
     4801 tggattctca ataagtacct cacgcaagcc cgtacacttc gtcgtgaaca tgttcgttcg
     4861 ggattccgcc tggcactcgc cgcggagagt gcgatcaaag ccggttggga accaacggtc
     4921 gtgcttgacg ggttgatatg tcaactcgcg gcgcgttgag gtcttgacga gtgcgagatt
     4981 cgacccgtac attatctctc ttgcgtatta cgcgtctcaa tcgatgtata ccgcaagcga
     5041 aaggataggt atccatggca aaacccattg tcgttactga tgatacgttc gaacaggaag
     5101 ttctgaagag cgatatcccg gtcgtagttg atttctgggc cgtctggtgc ggtccgtgtc
     5161 ggatgattgc tcccgttgtt gaagagcttg ccgtgcagta tgacggtaag gtgaagtttg
     5221 cgaaggtcga tgtcgatgat cagcagaagt acgcaggtca attcggcatt cgtagcattc
     5281 cgacgcttct catattcaag gatggcaagg tcgtcgatca gatcatcggc gccgtgccga
     5341 agggcgaact cgacaagaag gtcaaagcgc aactctaaac aaagacgcga gccaacgtag
     5401 ctcagttggt agagcagctc actcgtaatg agcaggtcgt cggttcaaat ccgaccgttg
     5461 gctccagtga atagccctcg tacgtgccat ac
//
LOCUS       DSCC01000157            1959 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039615, whole genome shotgun sequence.
ACCESSION   DSCC01000157 DSCC01000000
VERSION     DSCC01000157.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039615"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            109..>1959
                     /locus_tag="ENN56_01880"
     CDS             109..>1959
                     /locus_tag="ENN56_01880"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03781.14,HMM:PF13088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDS74268.1"
                     /translation="MSDVAEFTNSLGMIIRRIEPGRFLMGNDRPLADEIVRLGHWRYG
                     NLDERPVHWVTITEPFGLSETPVTNAQYEQFDPAHRELRGKMGFSTDDDEAVVFVNWH
                     DAVRFCEWLSEREEKPYRLPTEAEWEYACRAGTTTAYHTGDTLPPEFLTNAGSVWYPT
                     VRNPDIDHPVPLPVGKTPPNAWGLRDMHGLVEEWCGDWYGAYEEHVYVNPVGPTFGDF
                     RVTRGGSHSTEAYYLRSANRSGCLPEERHWYVGFRVAMGDEPQPARVRNADWIERHQW
                     NVISTVPESIETGPDADSPCFAGPLEYVNIPHDSFGPLYGTHNHDPGFAVCPNGDLLA
                     IWYTCVEEPGRELGIAASRRRFDRVAGRLAPTWDEPSIFWDAPGKNDHAPALWSDGAR
                     LYHFNGLSAGATWGPLATILRTSDDSGATWSSARIIIPEYGPRHMPIAGVFALRDGTI
                     VLPCDTPGGGKEGVEPGTALWLSQDGGETWYDAGGTIAGIHAGVVELADGSLLAYGRG
                     RDIDGMMARSVSNDRGKTWQYDASPFPGIRSGQRIVLMRMKGACEHGSDPLVLVSFAK
                     EPLDSPNAFRITDASGAKRPITGMFAAVSFDDGATWPFARPVTDDGPDRFI"
BASE COUNT          389 a          611 c          612 g          347 t
ORIGIN      
        1 ccgtagagac gagtatgaac tgattcgtct cccccttccc ccacaatccg cgcgtgtgtt
       61 tcgtgatggt cggagccaca ccgacctctc acggaaaggg cacaccgtat gtccgacgtc
      121 gctgaattca ccaactccct cggtatgatc atccgccgta tcgaacccgg gcgcttcctc
      181 atgggcaacg accgtccgct cgccgacgag atcgtgagac tcggtcattg gcgctacggt
      241 aacctcgacg agcgaccggt gcactgggtg acgatcaccg agccgttcgg actgagcgaa
      301 acgcccgtaa cgaacgccca gtacgagcag tttgatccgg cacatcgcga gttgcgtggg
      361 aagatgggat tttcgacaga tgacgacgaa gccgtcgtgt tcgtgaattg gcacgatgcg
      421 gttcggttct gcgaatggtt gagcgaacgc gaagagaagc cataccgctt gccgaccgag
      481 gccgaatggg aatacgcctg ccgcgcggga acgacgaccg cctaccacac tggcgacacc
      541 ctcccaccag agtttctgac caacgcggga agtgtgtggt atcccaccgt gcggaatccc
      601 gatatcgacc accctgttcc gttacccgtc ggcaaaacgc cgccgaacgc gtggggtcta
      661 cgcgacatgc acggactcgt cgaagagtgg tgcggcgact ggtatggggc gtatgaggaa
      721 cacgtgtacg tcaatcccgt gggacccacc tttggtgatt ttcgcgtcac gcgcggcggg
      781 agtcattcca ccgaggcata ctaccttcgc tcggcgaatc ggtccggatg cctcccggaa
      841 gaacggcatt ggtacgtcgg attccgcgtc gctatgggcg acgaaccgca gccggcacgg
      901 gtgcgcaatg cggactggat cgaacgtcat cagtggaacg tgatctcaac ggtgccggag
      961 tctatcgaga cgggacccga cgccgattcg ccgtgcttcg ccggaccgct ggaatacgtc
     1021 aacataccac acgactcgtt cggaccgctt tacggaacgc acaatcacga tccgggattc
     1081 gcagtctgtc ccaatggcga tctactcgcg atctggtata cctgcgtaga ggagcccggc
     1141 cgcgaactgg gaatcgccgc gtcgagacgc cgtttcgacc gagttgccgg aagactcgca
     1201 cccacgtggg acgaaccgtc gatattctgg gacgcacccg gcaagaacga tcacgcgccc
     1261 gcgctctggt cggacggtgc gcggctctac cacttcaacg gcctgtcggc aggcgcgacg
     1321 tggggaccgc tcgcgacgat ccttcgcacc tcggacgaca gcggcgcgac gtggtcctcg
     1381 gcgcggatca tcatccctga atacggtccg cggcacatgc caatcgccgg ggtgttcgca
     1441 cttcgcgacg gaacgatcgt gctcccgtgc gacacaccgg gtggcggaaa agaaggcgtt
     1501 gaaccgggca ccgcgctgtg gctcagccag gacggtggcg agacatggta cgatgcgggc
     1561 ggcacaatcg caggcatcca cgccggtgtc gtcgaattgg ctgacggcag cctcctcgca
     1621 tacggacgtg ggcgcgatat cgacggtatg atggcgcgca gcgtgtccaa cgatcgcgga
     1681 aaaacgtggc aatacgacgc gagccccttc ccggggattc gttccgggca acgcatcgta
     1741 ctgatgcgca tgaagggcgc gtgcgagcat ggaagcgatc cgcttgtgct cgtatcgttc
     1801 gcgaaagagc cgctcgactc gcccaacgcg tttcgcataa ccgacgcatc cggcgcgaaa
     1861 cgaccgatca cgggtatgtt cgccgcggtg tcattcgacg atggagcgac gtggccgttc
     1921 gcgcgaccgg ttaccgacga tggtcccgat cgtttcatc
//
LOCUS       DSCC01000158            4927 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1011414, whole genome shotgun sequence.
ACCESSION   DSCC01000158 DSCC01000000
VERSION     DSCC01000158.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1011414"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..442
                     /locus_tag="ENN56_01885"
     CDS             <1..442
                     /locus_tag="ENN56_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74269.1"
                     /translation="KNGDTFSEIYLSYLNDSRGARIPSTEFFTPFPPDEKFGFRRDVH
                     GVPEHCIRAYHLRDAKTGEAGPWLAGLTLEPSIVYEAWCSQRYGDIIVMIEEIHGKPV
                     RAGESFGAAHIVGYFDTIEEMHTLYERHRGSTALEVDADGWQFA"
     gene            490..1452
                     /locus_tag="ENN56_01890"
     CDS             490..1452
                     /locus_tag="ENN56_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_662285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74270.1"
                     /translation="MATYTFLDLAIDILEKAEKPLTYHEIWQRGEESGLTKKIDSTGK
                     TPWQTLGARLYVDVRDNDSSKIVKVGNRPARFYLKGRNVPLGTIEKIEIAEVQQKEPK
                     TTYSELELHPLLTYFAYTNPTFNRGRSILTKTIFHQKSKKSGYSEWTHPDIVGFHIPL
                     EDWDPDIIAFNRLSDNNLLRMFSFEMKKSLNKANYRESYFQAVSNSSWAHEGYLVAVD
                     ISKDDAFRSELERLASSFGVGIIHLDPTDIDSSRILYPARTRDSLDWETMNKLCEQNS
                     DFSTFVKDVKNALTNATIYPSVFDEVKKDIDEYIRKTLKITPST"
     gene            1530..2891
                     /locus_tag="ENN56_01895"
     CDS             1530..2891
                     /locus_tag="ENN56_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74271.1"
                     /translation="MSDLLFTFAAMATRTEGDLTALKGMERLAEVLHPHGVRITWLIT
                     PGIAERLREPLTKWHEENGDDIAIAPEGLRRDHTGFDLGTMQGQIDAMAAAAKDTRDR
                     VRKILPWTKADVAHGVHQNHVALQALEQAGFVGIWGFCFEQIEVDGITDRGIPWGFAY
                     IDSKCRTAPNTEPGGLISMEWTARDLLKSYHSGNPTVWSTDVNDAARSGLCRWRDCDY
                     LKRMFDEYYRNVRWNDLVVYQVHQEAHEMMPEFGCYSEEDCVEAMEMLDELAGHAMGH
                     LGVRSVSLSDAAAEYRSRNTMTAPSYMLWRDIPVGQYNDDYSRGTPRGPWPKTFLTYD
                     SECQLMFVDGGFEPRTIRNYRLRNHETGAPDQPYYAEPIIPRVRLKENNRSPYGAEIV
                     FEVEAPHAMPMGVTFWEDWGPYLWDSGEGILDHKIISGELMYLRMNVEAGKQEYRIKL
                     RRK"
     gene            complement(2972..4249)
                     /locus_tag="ENN56_01900"
     CDS             complement(2972..4249)
                     /locus_tag="ENN56_01900"
                     /inference="COORDINATES: protein motif:HMM:PF00877.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74272.1"
                     /translation="MAAPVVIISIRRVTAMKRYRIPIIFVQVFFALCSFPVLSHAAVA
                     SDDGVTAFSRLDEAKLRESMRSYAGTPYRYGGTSKNGIDCSGFTFAVYREQGITLPRT
                     VREQFRVGQSVSRSQLAVGDLVFFNTAGTGPSHVGIVSGPARFFHGSTSKGVIEDDLN
                     SDLWRRRFVGARRIASARTFTIRGDRVGASASDRVVLINRYPFTTYSLVNVPTNQVGE
                     ARSLSLGFTTNSSGDMVIAGRSSMWNRIELAGFLPVTGALDAGAPGVKQPDGLVKLRI
                     NDQWHRIPGFAIGYDTRRARLIEETIYGDSVRVTNRRGLFIVGSGNLTYRQGFLIGET
                     RFHAGASIASVGNVESTEDVGVFVGLEQQLLRRIALVGEVDNLFGERAAHINAGIRIT
                     VTDDAVLEYAVTAIGPRHYRPEKVLRFSFHIPY"
     gene            4442..>4927
                     /locus_tag="ENN56_01905"
     CDS             4442..>4927
                     /locus_tag="ENN56_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74273.1"
                     /translation="MSDTNRHDVILAPATDEFPRNTEGSVVRLETGRLLAAWTAFERG
                     TGDDTTAHIKARISGDDGVTWGEPFVLMENVAAQNTMSVSLHREQSGGLLMAVLEKHG
                     PDDCRLVVRRAVKEVREWSSPITVTRKPGYYIMNNDRLAEDRAGRIYAPIAFTREPVW
                     SG"
BASE COUNT         1131 a         1358 c         1444 g          994 t
ORIGIN      
        1 caagaacggc gatacgttca gcgagatcta cctcagctac ctgaacgact cacgcggtgc
       61 gcgtatcccg tcgaccgaat tcttcacacc gtttcctccc gatgagaagt tcggattccg
      121 ccgcgacgtg catggggtgc cggagcactg cattcgcgcg tatcatttgc gagatgcgaa
      181 gaccggcgag gcgggaccgt ggcttgccgg gctgacatta gagccatcga tcgtgtacga
      241 ggcgtggtgc agtcagcggt acggtgatat tatcgtcatg atcgaagaga ttcacggcaa
      301 accggtgaga gccggcgagt cgtttggcgc cgcccatatc gtcgggtatt ttgacacgat
      361 cgaagaaatg cacacgcttt acgagcgcca tcgcggcagc acggcactgg aagtcgatgc
      421 cgacggctgg cagttcgcgt aatagattcg tgtagacgaa tacgtatacc gcgacgagga
      481 ggtatctcca tggcgaccta tacgtttctt gatctcgcga tcgacatttt ggaaaaagcg
      541 gagaaaccgc taacctatca cgagatatgg cagcgtggtg aggaatccgg gcttacgaaa
      601 aagatcgata gcacgggaaa aacgccatgg cagactctcg gcgcgcgatt gtacgtagac
      661 gttcgtgaca atgatagttc gaagatcgtt aaggtcggaa accgaccagc gcgtttctat
      721 ctcaaaggga gaaacgtgcc attgggaacg atcgagaaga ttgagatcgc agaggtccaa
      781 caaaaggagc cgaagaccac ctattcggaa ctggagcttc atccactttt gacgtatttc
      841 gcgtatacga acccgacctt caatcgtgga aggtcgatac tgacgaagac gatttttcac
      901 cagaaatcga agaaatcggg gtacagcgaa tggacacacc ccgatatcgt cgggtttcat
      961 ataccactcg aggactggga tcccgatatc atcgcgttca atcgtctatc cgacaacaat
     1021 cttcttcgca tgttttcatt tgagatgaag aagagtttga ataaagcgaa ctatcgcgaa
     1081 tcgtatttcc aggcagtatc gaattcgtca tgggcgcacg aagggtatct cgttgctgtc
     1141 gacatctcga aagacgacgc gttccgctcc gaacttgaac gtctcgcatc ctcgttcggc
     1201 gtaggcatca ttcatctcga tccgaccgat atcgacagtt cgcgcatcct gtatccggcg
     1261 cgaacgcggg actcgctgga ttgggaaacg atgaacaaac tctgcgaaca gaacagtgac
     1321 tttagcacgt ttgtcaaaga cgtcaaaaac gctttgacaa acgcaacgat ctatccttct
     1381 gtattcgacg aagtgaagaa ggatatcgac gagtacatcc ggaaaacatt gaagatcaca
     1441 ccgagtacgt agacagcgcg taaccgagaa aaaagacggg ttacgcatcg tgccacgaaa
     1501 cacttaactc gacaaacgaa gggaacatca tgtccgactt gttattcacg ttcgccgcca
     1561 tggcgacgcg taccgagggc gatctcaccg cgctcaaggg aatggaacgc ctcgccgagg
     1621 tgctccatcc acacggcgta cgcatcacct ggctgatcac gccgggtatc gccgaacgac
     1681 tccgtgagcc gcttaccaaa tggcatgagg agaacggcga tgacatcgcg atcgcaccgg
     1741 aagggctacg ccgcgatcac acgggattcg acctcggcac gatgcagggg cagatcgacg
     1801 ccatggctgc cgcggcgaag gatacgcggg accgcgttcg caagatcctc ccatggacga
     1861 aggcagatgt cgcacacggc gtgcatcaga atcacgtcgc gttacaggca cttgaacagg
     1921 ccggtttcgt cggtatctgg ggattctgct tcgaacagat cgaagtggac ggcatcaccg
     1981 atcgcggcat tccgtggggg ttcgcgtaca tcgattcgaa gtgccgcact gcgccgaata
     2041 ccgaaccggg cggcctgatt tccatggagt ggacggcacg cgacctgctg aagtcatacc
     2101 acagcggcaa tccgacggta tggagcaccg acgttaacga cgccgcgcgc agtggcctct
     2161 gtcggtggcg tgattgcgat tacctgaagc gtatgttcga cgaatactac cgcaatgtcc
     2221 gttggaacga cctcgtggtg tatcaggtgc atcaggaagc tcacgagatg atgccggagt
     2281 tcggctgcta cagcgaggaa gactgtgtcg aggcgatgga gatgctcgac gagctcgccg
     2341 gacacgccat ggggcacctc ggcgtgcgtt cggtatcgct gagcgacgcg gcggcggagt
     2401 atcgctcgcg caataccatg acggcaccgt catacatgct gtggcgcgat atccccgtcg
     2461 gtcagtacaa tgacgactat tcacgtggaa cgcctcgcgg tccgtggccg aagacgttcc
     2521 tcacatacga ctcggaatgt cagctcatgt tcgtcgacgg cggattcgag ccgcgcacga
     2581 tccgcaatta ccgtctgcgg aaccatgaga ccggcgctcc cgatcagccg tattacgctg
     2641 aaccgatcat cccgcgcgta cggctcaaag aaaacaaccg ttcgccctac ggtgccgaga
     2701 tcgtgttcga ggttgaagct cctcacgcga tgcccatggg cgtaacgttc tgggaagact
     2761 ggggtccgta tttgtgggat tcgggcgaag gcatcctcga ccacaagatc atttcgggcg
     2821 aactcatgta tctccggatg aacgtcgagg cgggcaagca ggaataccgg ataaagctcc
     2881 ggcggaagta acacgagcga ccacaccaac aaagaaggga acccgacacc gtcaggctcc
     2941 cttctttccg tgtgccgtga tgggtgggcg atcaataggg aatgtggaag ctgaaccgca
     3001 gcaccttttc cgggcggtag tggcgcggac cgattgccgt aacagcgtat tcgagtaccg
     3061 cgtcgtctgt cacggtgatt cggatgcccg cattgatgtg tgccgcgcgt tcgccgaaga
     3121 gattatcgac ttctccaaca agggcgatac gccggagtag ctgctgctca aggccaacga
     3181 acacgcccac atcttccgtg ctctccacgt tacccaccga agcgatgctc gcacctgcgt
     3241 ggaagcgtgt ttcaccgatc aggaagcctt gccggtacgt gagattcccg cttccgacga
     3301 tgaagagtcc gcgtcgattt gtcacgcgga cggagtcgcc gtagatcgtc tcctcgatca
     3361 gtcgcgcccg acgcgtatcg tagccgatcg caaagccggg aatgcggtgc cactggtcgt
     3421 tgattcgcag ctttaccaat ccgtcaggct gtttgacgcc gggtgcaccc gcgtcaagcg
     3481 cgccggttac cggcaggaat ccggccagtt cgatgcgatt ccacatgctc gaacgacccg
     3541 cgataaccat atcgccggac gagttcgtcg tgaatcccaa cgatagagaa cgtgcttcgc
     3601 cgacttgatt cgtcggcacg ttgacgagtg aataggttgt gaacggatac cggttgatga
     3661 gtacgacacg gtccgatgcc gatgctccaa cgcgatcacc cctgatggta aatgtccgtg
     3721 ccgatgcaat ccggcgtgca ccgacgaacc ggcgcctcca gaggtcgctg ttcaggtcgt
     3781 cttcgattac gccttttgac gtgctgccgt ggaaaaaccg cgcgggtccg gaaacgattc
     3841 cgacatgcga cggtccggtc ccggcagtgt tgaaaaagac gagatcaccg acggccagtt
     3901 gcgatcgcga gaccgactgg ccgacgcgga actgttcgcg taccgtacgc ggcagagtga
     3961 tcccctgttc ccgatatacg gcgaaggtga agccggagca gtcgatgccg tttttcgacg
     4021 ttccgccgta ccggtatggc gtaccggcgt acgaccgcat actctcgcgg agtttcgctt
     4081 cgtcgagtct ggagaaggcg gtaactccgt catcggaggc gacggctgcg tgactcaaca
     4141 cagggaagga gcagagtgcg aagaaaacct gaacgaagat tattggaatc cggtaccgtt
     4201 tcatcgcggt cactcggcgg atgctgatga taaccacagg tgccgccaca agcggtaccg
     4261 gcggttttcg tatgtcgggt tgagtacagt ataggcgggt cgccggggaa tgggcaagcc
     4321 cgattcggcg aatcgtacgt ttgatccttg ttccattctc ttttgcttcc tacgttcctc
     4381 gtatcttttg actgttcgat tcgaaacaga ccgagagaaa atgtaaaaca acggagttat
     4441 catgtcagat acgaatcgcc acgatgtcat ccttgctccg gcgaccgacg agtttccgcg
     4501 caataccgag ggaagcgtcg tccggcttga aacgggccga ctacttgcag cgtggacagc
     4561 attcgagcgc ggcaccggcg acgacaccac cgcacatatc aaagcgcgta tatccggtga
     4621 cgacggcgtg acgtggggtg aaccgttcgt tctgatggaa aatgtcgcgg cgcagaacac
     4681 catgtcggtg tcgctgcatc gggaacagtc cggtggcttg ctcatggccg ttctcgaaaa
     4741 acacggccct gacgactgcc gtctcgttgt tcgtcgtgcg gtgaaggagg tcagagaatg
     4801 gtcgtcgccg attacggtga cccgtaaacc gggctactac atcatgaaca acgaccggct
     4861 cgcagaagat cgtgcgggtc gtatctacgc gccgatcgcg tttacgcgtg agccggtgtg
     4921 gtctggc
//
LOCUS       DSCC01000159            1824 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043683, whole genome shotgun sequence.
ACCESSION   DSCC01000159 DSCC01000000
VERSION     DSCC01000159.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043683"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="ENN56_01910"
     CDS             complement(<1..1763)
                     /locus_tag="ENN56_01910"
                     /inference="COORDINATES: protein motif:HMM:PF00984.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74274.1"
                     /translation="MTDGYVRSWLEESIALYNVVDAHGLNEAVDTRMKAYNREIETLG
                     AALIEELGLGVEVDTIMATEGVPKSSAIRRIISANRTVQKQVALLAVLREVSGHTKAD
                     HVDPFPVDTYVENHRDELEGAALRDVIARNQREIEEAVAELRGKDHSTSESELQRQVV
                     QSDDDYRADLDTYVRFAAREAVLEAWEREHPDWKLRERWERWLRQHQRLALTTARKEV
                     VSEHGLNHLTMDPRYYFQATAGNKRFHLLYTPSRVDLGPRERESVETWAQWVGGRDTA
                     AAQVGRRIYGLINKSVKRFDSLTEPEVLKTGENASMASHFAYSNAMALMVNATGRGDF
                     EELGDQMSLREDRKIHPAGEGYGGYCVPKDGLFLEFVLTLTESVKLRQLGIEDRFHEA
                     VSTLAARVLTRRDDFATDLEWETWAEKKIADQNGLRDLFELRDGHIPVFQITRLAAVL
                     DELGQPPLREHRDVVKTLSARWGVHTMIAGAEHVNRFMPFYKAWLTYDALDQARNAEK
                     NRKLPEARDAVIVLSAEYKPDTQDGRFSVGMRKYEIYTGTDDHLRYSLGSEASLIASL
                     MIDGWDRTARKRGTDDPELAK"
BASE COUNT          314 a          550 c          531 g          429 t
ORIGIN      
        1 atctttgcca gttccggatc gtcggttcca cgctttcgtg ctgtccggtc ccacccatcg
       61 atcatgagtg atgcaatcag actcgcttcg ctgccaaggc tgtagcggag atgatcgtcg
      121 gtgccggtgt agatctcata tttccgcata ccgacgctga agcgtccgtc ctgcgtatcc
      181 ggcttgtact cggcgctcag cacgatgacc gcgtcgcgcg cctcggggag tttgcggttt
      241 ttctccgcgt ttcgcgcttg gtcgagggcg tcgtacgtga gccacgcttt gtaaaacggc
      301 atgaacctgt tgacgtgctc ggctcctgca atcatcgtgt gcacgcccca acgcgcgctg
      361 agcgtcttca cgacatcgcg gtgttcgcgc aacggtggct gaccgagctc atcgagcacc
      421 gcggcaagtc gcgtgatctg gaataccggt atgtgtccgt cgcgcagctc gaagagatcg
      481 cgcaagccgt tctggtcggc gattttcttc tccgcccacg tttcccactc aaggtcagta
      541 gcaaaatcat cccgccgcgt aagaacgcgg gcggcgagag tcgaaacggc ctcgtggaaa
      601 cggtcctcga taccaagctg gcgaagcttc accgactcgg tgagggtgag cacgaactcg
      661 agaaagagtc catctttcgg cacacagtac ccgccgtatc cctcgcccgc cgggtggatt
      721 ttccggtcct cgcgcaagct catctggtcg ccaagctctt cgaaatcgcc gcgtccggtg
      781 gcgtttacca tgagtgccat ggcgttcgag tacgcgaaat gcgacgccat cgatgcgttt
      841 tcgccggttt tcaatacttc cggctcggtg agactgtcga atcgttttac gctcttgttg
      901 atgagaccgt agatccggcg gccgacttgc gcggcggcgg tatcgcgacc accgacccac
      961 tgcgcccacg tctcgacgga ttcgcgttcg cgcggcccga gatcgacgcg gcttggcgtg
     1021 tagaggagat ggaagcgttt gtttcccgcg gttgcctgaa aatagtaccg cgggtccatc
     1081 gtcaggtgat tcaacccgtg ctctgaaacg acttcctttc gcgcggtcgt caatgcgagc
     1141 cgctggtgtt gacgcagcca acgttcccac cgttcgcgga gcttccaatc aggatgttca
     1201 cgttcccatg cttccagaac ggcttcgcgc gcggcaaacc gtacgtacgt atcgaggtcg
     1261 gcacggtaat cgtcatccga ctggacaact tgacgctgta actcggattc tgacgtcgag
     1321 tgatctttcc cgcgtagctc ggccactgct tcttcgattt cacgctgatt ccgcgcgatg
     1381 acatcgcgga gggctgcgcc ttcgagctcg tcgcgatggt tttcgacgta tgtatccacg
     1441 ggaaatggat cgacgtgatc cgctttcgta tgcccagata cctcgcgcaa tactgcgagc
     1501 agcgcgactt gcttctgtac tgtccggttg gcgctgataa tccggcgaat cgccgacgat
     1561 ttcggtacgc cctcagtcgc catgattgtg tcgacttcca cgccgagtcc gagctcttcg
     1621 atgagcgctg ctccgagcgt ttcgatttcg cggttatacg ctttcatgcg cgtatctacc
     1681 gcttcgttga gaccgtgtgc gtcgacgacg ttgtagagcg caatactttc ctcgagccaa
     1741 ctccgcacgt acccgtcggt catatttgcc gattgtgtgg tgagaacgtg tagagcgggt
     1801 agcggtcgtc gcggcaggtc ggcg
//
LOCUS       DSCC01000160            2271 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032376, whole genome shotgun sequence.
ACCESSION   DSCC01000160 DSCC01000000
VERSION     DSCC01000160.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032376"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..542)
                     /locus_tag="ENN56_01915"
     CDS             complement(<1..542)
                     /locus_tag="ENN56_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDS74275.1"
                     /translation="MPMPGTRSSSSNPIRSPMPEREPVTVVDYLNLAVDFLAKRGIEN
                     PRLHAELLLGDVVGLKRIELYLQFDRPLTTTEIHRYRELLRRRSTGEPVQYILGHTEF
                     YGRRFAVNPSVLIPRPETEVLVEQTLNRLRGVAEPDILDIGTGSGAIAVTIAAERPDA
                     RVVATDISADALDTARANAE"
     gene            complement(484..1512)
                     /locus_tag="ENN56_01920"
     CDS             complement(484..1512)
                     /locus_tag="ENN56_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74276.1"
                     /translation="MSFTTTAYARIGLLGNPSDGYYGKTIACAIRNFAATVTLEESEC
                     FVCEPHPEFDPREFASIDALHDSAVRDGYYGGLRLLLASAKKFAEYCRRHDITLPTRN
                     CTLRYDTNIPRQVGLAGSSAIITATIKALRTFYGINDEQLPKVLQPNLVLSVEEEELD
                     IRAGLQDRVAQTYGGLVYMDFDRKLLESRGYGEYEELDPSLLPPLFLAYLDEPKDSGK
                     THSNVRERWKSGDADVVSAMRAFAEFTTDGRRALLERDYTTLADLMDRNFDLRRRIFG
                     DAVIGAHNLEMIGIARDHGAAAKFSGSGGAVVGTPRESGSMDAIRTAYADAGYSFVIV
                     EPDTKSDA"
     gene            1900..2175
                     /locus_tag="ENN56_01925"
     CDS             1900..2175
                     /locus_tag="ENN56_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74277.1"
                     /translation="MQKSFLTAVVAGGLLVGLMAGCTPKPNEEQLQELNRVCAAADEA
                     ESALEQTRRDLNSVERQLASARQSLQERQAYLNSVRQNLVALERDAQ"
BASE COUNT          464 a          620 c          711 g          476 t
ORIGIN      
        1 cactccgcgt tcgcgcgggc agtatcgagc gcgtcggcag agatatccgt cgcgacaacg
       61 cgagcgtccg gacgttccgc cgcaatcgtc accgcgatgg cgcccgatcc ggtgcctata
      121 tcgaggatgt ccggttccgc gacgcctctc aagcggttga gtgtctgctc gacgagtact
      181 tccgtttcgg gacgcggaat gagtactgac gggttcacgg cgaacctgcg accgtagaac
      241 tcagtatggc cgagaatgta ctgtaccggc tcgcctgtcg agcggcggcg gagaagctcg
      301 cggtaccggt gaatctcggt cgttgttagc ggtcggtcga actggagata tagctcgatg
      361 cgcttcaatc cgacgacgtc gccgagcagc aactccgcgt gcagccgcgg gttctcaatg
      421 ccgcgttttg cgagaaaatc caccgcgagg ttgaggtagt cgacgacggt gaccggttct
      481 cgttcaggca tcggacttcg tatcgggttc gacgatgacg aacgagtacc cggcatcggc
      541 atacgcagtg cggatagcgt ccatactccc cgattcgcgt ggcgtaccga cgaccgcgcc
      601 gcccgatccg ctgaatttcg ccgccgcacc atgatctcgt gcgataccga tcatctcgag
      661 gttatgcgca ccgattaccg catcaccgaa aatccggcga cgtaaatcga aattgcgatc
      721 catgagatcg gcaagtgtgg tatagtcgcg ctcgaggagt gctcgacggc catcggtggt
      781 gaactcggcg aacgcacgca tggcgctcac gacgtcggca tcgccgctct tccaccgttc
      841 acggacattg ctgtgcgtct tgccggaatc tttcggttcg tccaagtatg caaggaacag
      901 tggtgggaga agactcggat caagttcctc atactcgcca tatccgcggc tttcgaggag
      961 tttccggtcg aaatccatgt agacaagtcc gccgtaggtc tgcgcgacgc ggtcctgtaa
     1021 acccgcgcgg atatcgagct cttcttcctc gacactcagc acgagattcg gctgaagcac
     1081 cttggggagt tgctcgtcgt tgatgccgta aaacgttctc agcgccttga ttgtcgcggt
     1141 aataattgcg ctggaaccgg cgaggcctac ttgacgcggg atattcgtgt cgtaccgtag
     1201 tgtgcagttg cgcgttggta acgtgatgtc gtgacgccga caatactcgg cgaacttctt
     1261 cgccgacgcg agcaagaggc ggagaccgcc atagtatccg tcgcgtacgg cgctgtcgtg
     1321 gagcgcgtcg atcgaggcga actcccgcgg gtcgaactcc gggtgcggct cgcagacgaa
     1381 gcactccgat tcctcgagag tgacggtggc ggcgaaattt cgaatggcac acgcgatggt
     1441 tttgccgtag tacccgtcgg aggggtttcc aagcagaccg atacgggcgt acgcggtcgt
     1501 tgtgaatgac atcatgcagg tacctctgga acttgcagta gattttggtg cgatctacct
     1561 ttcgtacgtt gcggctgaac gacgatccgt ctaacgccgc atgaacggac atcgcgagta
     1621 tccgattcgg taaaatcgtc atcgcgaaat gagtggtgcc gatgaaagta tagagaactt
     1681 tcgtcggaat caccatccat acgcgattga ccggtgcgtt tggattgcgt atagtacggg
     1741 tgtctgtcgc atcacggatt cgcgggaatc tgcgaattgg gtcagggtgc gacgtatggc
     1801 attctcaaac attgaatgtt ttcgcacgca agagaaagat gctctccggt ggggccgaat
     1861 ggtctccggg ggcagtatcg cgtagcacgg gaggatgcaa tgcagaaatc gttcttgacc
     1921 gcggtggttg ccggcggtct cctcgtaggg ctcatggccg gctgtacgcc aaagccgaac
     1981 gaagagcagc tacaggagct gaatcgggtg tgcgccgctg cggatgaggc ggaaagtgca
     2041 cttgagcaga cacggcgtga tctcaacagc gtcgagcgtc agctcgcatc agcacgccag
     2101 tcgctccaag agcgtcaggc gtacctgaac agcgtacgtc agaatcttgt cgcgctcgag
     2161 cgcgatgcac agtaacttca gcgggaatga tccgaagtcg attcggcatc gggttcggtt
     2221 tccggggaag tgacggacgg acatgaaggt acgcaggcga cacattatgg g
//
LOCUS       DSCC01000161            4558 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1012711, whole genome shotgun sequence.
ACCESSION   DSCC01000161 DSCC01000000
VERSION     DSCC01000161.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1012711"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..799)
                     /locus_tag="ENN56_01930"
     CDS             complement(<1..799)
                     /locus_tag="ENN56_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74278.1"
                     /translation="MAHSYTPGLRVTPFTALKRRRILPLKGGVTVEVGQRVERADIVA
                     RTDLPGDVASVNVVNRLGIDASQINQFMLKKEGDTIRREEIIAENRPLIKWFKTVVKS
                     PIDGTVESISSVTGQVLLRHPPRPVAVAAYVDGEVVEVIPNEGVVVETEGAFVQGIFG
                     IGGEAWGPVKVISEAPGDIVTPERITDDCTGCVLIIGAIAGIDVIRRAVQVKAAGIIA
                     GGINADDLKNILGFDLGVAITGTEEIGTSVIVTEGFGSIAIAHRTYEI"
     gene            complement(840..2636)
                     /locus_tag="ENN56_01935"
     CDS             complement(840..2636)
                     /locus_tag="ENN56_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase"
                     /protein_id="HDS74279.1"
                     /translation="MSETPKLDVIIATDCGSTTTKAILIEKVDGQYRQTYRGEAPTTV
                     EAPFEDVTRGVLNAIQEIEELSGRTILDGEQIITPAEGDRGVDIYISTSSAGGGLQMM
                     VAGVVKSMTGESAQRCALGAGAIVMDVLASNDGRLPHQKIERIRQLRPDMVLLSGGTD
                     GGTVTHVVALAEFIAAADPKPRFGIGFELPVIYAGNVDAREQIDNILGEKTSLTITDN
                     LRPTLEAENLMPARHKIHDLFLEHVMAQAPGYRKLMGWTGAPIMPTPGAVGVIMQTIA
                     RNQGINIVGVDIGGATTDVFSVFDEVFNRTVSANLGMSYSVSNVMAEAGLDNIMRWVP
                     FDIDEQDLRNRVKNKMIRPTTIPQMLDELKIEQALAREALRLAFIQHKALAVGLKGVA
                     KERTLSDVFAQSEDESLIDMMRLDLLVGSGGVLSHAPRRQQSMQMMIDSFQPEGVTRL
                     AVDSIFMMPHLGVLSTVNEQAATEVFEKDCMIYLGTCVAPRGRGKRDTKIATYSLTLP
                     DGTTKDATLSVGDLLYFPLPLDQTAQIHVTPQRGWDFGAGAGKEIEATLHGGVVGLVI
                     DGRSRPIQFADNRSEQVDDIKRWNAATDQYPA"
     gene            complement(2633..3025)
                     /locus_tag="ENN56_01940"
     CDS             complement(2633..3025)
                     /locus_tag="ENN56_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74280.1"
                     /translation="MPLHETASLWTSLKRTMNHAFAMPPPLDLSDNDRALLDRIADMI
                     VRRRLAAPAMMILELARPLNFVAGQFLHFVGPFATLLLKEREYRRFAEILEHRQGIDA
                     LLNAINQKASDTDSVGINTPRNTHGTVE"
     gene            complement(3438..>4558)
                     /locus_tag="ENN56_01945"
     CDS             complement(3438..>4558)
                     /locus_tag="ENN56_01945"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:PF00342.17"
                     /note="catalyzes the formation of D-fructose 6-phosphate
                     from D-glucose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDS74281.1"
                     /translation="HAYHNDLPAETRGGLRLEVPDNVDPDRLADLFDVLDLSRTVFNV
                     ITKSGSTAETTSTFLVIKNRLESDPKIGANWTKHVVITTDPEKGDLRDLAKAWKLPAF
                     DIPAGVGGRFSVLTPVGLVSAAATGIDISELLRGAGDMAERIRTTSIKANPAYISAAI
                     HYLLYHHTAPKRMAVMMPYSQRLRDVADWFRQLWAESLGKERDLSGNTVNVGPTPIKA
                     LGVTDQHSQVQLYAEGPNDKLYSFIAVDRFKTDIDFPEDRDLARYNAFNYFAGKTMGE
                     LLNYEREATTLALTRKGRPNLTINVPEVTPYTVGQLYMLLEVQTVVAGALFGIDPFDQ
                     PGVEAGKVATYALMDRPGYEEEKSQIERELAGRGRIVL"
BASE COUNT          899 a         1308 c         1297 g         1054 t
ORIGIN      
        1 ggatttcgta cgtccggtgt gcgatggcga tcgatccgaa cccttccgtg acgatgacac
       61 tcgtaccgat ttcctcggtg ccggtgattg cgacaccgag atcgaatccg aggatattct
      121 tcagatcgtc cgcgttgatt ccaccggcga tgatacctgc ggcttttacc tgtaccgcgc
      181 ggcgtatgac gtcaatacca gcgattgcgc cgataatcaa cacgcaaccc gtacaatcat
      241 ccgtgattcg ttcaggcgtt acgatatcgc ctggtgcttc cgagataacc tttaccggtc
      301 cccacgcttc gccgccgatg ccaaagattc cctgtacgaa cgcgccttca gtctctacga
      361 cgactccctc gttcggaatc acttcgacga cttctccgtc aacgtacgcc gccactgcaa
      421 cggggcgcgg cggatggcga agcagcacct gtccggtcac cgatgagatg ctttcaaccg
      481 tgccgtcgat cggcgacttt acgaccgtct tgaaccattt gatcaacggt cgattctccg
      541 cgatgatttc ctcacggcga atcgtatcgc cttctttctt cagcatgaac tggttgatct
      601 ggctggcatc gatcccaagg cgattcacca cgtttaccga agcgacgtcg ccgggaaggt
      661 cggtgcgcgc gacgatatcg gcgcgttcga cccgctgccc gacttcgacc gtgactccgc
      721 ctttcagcgg aagaatacgg cgacgcttta gcgcggtaaa cggtgttacg cggagtcccg
      781 gagtatacga atgtgccatg tgcgagcgtt cctcgaataa aagcccaaac cgctatctac
      841 tatgccgggt attggtccgt tgcggcattc cagcgcttga tatcgtcgac ttgctcggac
      901 cggttgtcgg cgaattgtat cggtcgactg cgtccgtcga tgacaagtcc gacgactccg
      961 ccgtggagcg tcgcttcgat ctccttgcct gctcccgcgc cgaaatccca accgcgttgt
     1021 ggcgttacgt ggatttgcgc ggtctgatcg agtggcagtg ggaaatagag gagatcgcca
     1081 acagacagtg ttgcgtcttt cgttgtaccg tcgggaagcg tgagcgaata ggtcgcgatt
     1141 ttcgtatcgc gcttaccgcg tccacgtggc gcgacacagg tcccgagata gatcatgcaa
     1201 tccttctcga atacctcggt cgccgcctgt tcgttcaccg tggaaagtac accgagatgc
     1261 ggcatcatga agatgctatc cacggcgaga cgggtgacac cttccggttg aaacgaatcg
     1321 atcatcatct gcattgactg ctggcggcgc ggcgcatggc tgagcacacc gccggaaccg
     1381 acgaggaggt cgagtcgcat catgtcgatg aggctttcgt cctccgattg tgcgaagacg
     1441 tccgatagcg tgcgttcttt cgcgacgccc tttaggccga ccgcgagcgc cttgtgctga
     1501 atgaacgcaa gccgaagcgc ctcgcgtgca agcgcttgct cgattttcag ttcatcgagc
     1561 atctgcggaa tcgttgtcgg gcggatcatt ttgttcttga cgcggttccg aagatcctgc
     1621 tcgtcgatat caaacggcac ccagcgcatg atgttgtcga gaccggcctc cgccatgacg
     1681 ttcgatacgc tataggacat gccgagattc gcactcaccg tacggttgaa gacctcgtcg
     1741 aacacgctga acacgtccgt cgtcgcgccg ccgatatcca ctccgacgat attgattccc
     1801 tggtttcgcg cgatcgtctg cataattacg ccgaccgcgc ccggtgtcgg cataatcggt
     1861 gcgccggtcc agcccatcag ctttcggtac cccggtgcct gcgccatgac atgttcgagg
     1921 aacaggtcgt ggattttatg gcgagcgggc atgaggtttt cggcttcaag cgtcgggcgg
     1981 aggttgtcgg taatcgtaag agaggtcttt tcgccgagga tgttatcgat ttgctcgcgc
     2041 gcatcgacat ttccggcgta tatcacgggg agctcgaatc caataccgaa acgtggcttc
     2101 ggatcggcgg ctgcaatgaa ctcggcaagc gcaacgacgt gtgttaccgt tccgccatcg
     2161 gttccgcccg agagcagcac catatcggga cgcaattgac ggatacgttc gattttctgg
     2221 tgtggcagac gtccgtcgtt cgacgcgagc acgtccatta cgatcgcacc ggcaccgagt
     2281 gcgcaccgct gtgcgctttc gccggtcatt gacttgacga cgcctgcgac catcatctga
     2341 agtccgccgc ctgctgacga tgtcgaaatg tagatatcta caccgcgatc accttcggcg
     2401 ggcgtgataa tctgctctcc gtcgagtatc gttcgaccgc tgagttcctc gatttcctgg
     2461 atcgcgttga gtacaccgcg tgtgacatct tcgaacgggg cttctacagt cgtcggagct
     2521 tcgccgcggt atgtctggcg atattgaccg tcgacctttt ctatgaggat tgctttcgtc
     2581 gttgtgctgc cgcaatcggt tgcgatgatg acgtcgagtt ttggagtctc actcattcca
     2641 cggttccatg tgtattgcgc ggtgtgttga ttccaacgga atctgtgtcg ctcgcctttt
     2701 ggttgatcgc gttcaacagc gcgtcaatgc cttgacggtg ttcgagtatc tccgcgaaac
     2761 gcctgtattc ccgttctttg agcagcaggg tggcgaatgg gccgacgaag tggaggaact
     2821 gacccgcaac gaagttcaac ggccgagcaa gctccaatat catcatcgcc ggagcagcca
     2881 gacgccgccg aacgatcatg tcggctatgc ggtcgagtaa ggcgcgatcg ttgtcactca
     2941 aatccagtgg tgggggcatc gcgaacgcgt ggttcatcgt tcgcttgaga ctggtccaga
     3001 gtgacgcggt ttcgtgcaat ggcatacaca agatctcgct gttgcgggca tcgcagatac
     3061 aacccgcgcc atgcatcgag acggcgcgga cgtggaaacg ttccgagaaa aacaccttca
     3121 tctcgaagcg aaacccgtcg gtaccgatcc cacggatact tccgcgagta tccgcagaat
     3181 ctcgtctgta cgccatccgc gttgataacg tatacagtag gaaaaagata ccgcgatata
     3241 ctgccgaaaa gaaaggtgac cgatactatt ccgatccaga ccgaccccat cccaacgccg
     3301 gcaacgaccg atgcgcctat cagaaaagcg atgagcacgg acctccgcgc gagtgaatca
     3361 tcggcccagg gatgactctt ccatgtgacc gcgcgtattg gttcgccggt catttgcgtt
     3421 ccgtcgaaat gccgcactta cagaacgata cgtccgcgtc cggcgagttc gcgctcgatt
     3481 tgggatttct cttcttcata acccggacga tccatgagcg catacgtcgc aaccttcccc
     3541 gcctcgacgc ccggctgatc aaatgggtcg atgccgaaca atgcgcccgc gacgacggtt
     3601 tggacttcga gcagcatgta gagctgaccg accgtatagg gagtgacttc cgggacgttg
     3661 attgtaaggt tcggacgtcc cttccgtgtc aacgcaagtg tcgtcgcttc gcgttcgtaa
     3721 ttcaagagct cgcccatggt cttgccggca aagtagttga acgcgttgta gcgcgcgagg
     3781 tcgcggtctt ctgggaaatc gatgtctgtt ttgaaacgat caacggcgat gaagctgtag
     3841 agcttgtcgt tcggaccttc cgcgtaaagt tgaacctgag aatgctggtc cgttacaccg
     3901 agtgctttaa tcggtgtcgg tccgacgttg accgtgttcc cgctgaggtc tcgctctttc
     3961 ccgaggcttt ccgcccacaa ctgtcggaac caatctgcga catcgcgaag acgttgagaa
     4021 tacggcatca tgacagccat ccgcttcggt gcggtgtggt ggtagagcaa gtagtgaatt
     4081 gccgcactga tgtacgctgg attcgctttt atcgacgttg tgcggattcg ttcagccatg
     4141 tcaccggcgc cgcgaagcag ctcgctgata tcgatccccg ttgcggctgc ggaaacgagc
     4201 ccgaccggcg tgaggacgct gaaacgcccg ccgacacctg cggggatatc gaacgcgggg
     4261 agcttccacg cttttgcgag gtcccgaaga tcgccttttt ccggatcggt ggtgatcacg
     4321 acgtgcttcg tccagtttgc gccgatcttc ggatcgctct ccagtcgatt cttgatgacc
     4381 aggaatgtac tggtcgtttc ggcagtgctc ccggatttcg taataacatt gaaaacggta
     4441 cgggagagat cgagcacgtc gaacaggtct gcgagacgat caggatcgac gttgtccggt
     4501 acttcgaggc ggagtcctcc acgcgtttcg gcaggcagat cgttgtggta cgcgtgac
//
LOCUS       DSCC01000162            4675 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1012307, whole genome shotgun sequence.
ACCESSION   DSCC01000162 DSCC01000000
VERSION     DSCC01000162.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1012307"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="ENN56_01950"
     CDS             complement(<1..1065)
                     /locus_tag="ENN56_01950"
                     /inference="COORDINATES: protein motif:HMM:PF13088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74282.1"
                     /translation="MPQGIRVDAIHGTICDLTGEKFGYFGWPSVARTESGRMYAIASG
                     LRWGHVCPWGKTVLFTSEDSGATWSRPRVINDTPLDDRDAGIISLGGEKVLLTWFTSD
                     TKNIANVENAPEEIRDEWRNAIDAIPDSVREKWRGSWVRTSVDGEAWSDFIRAPVNTP
                     HGPIRLESGDLLYFGKQWWMPGMGDPMEHVGDMRAAISRDDGYTWEELGSVPLPPEIS
                     PKNLHEPHVVETTSGRLIAMIRYQHSGDAKAYESFSLLQSESDDGWKTWTEAHHTGVY
                     GSPPHMIRHSSGAIVCVYGYRREPFGHRVMISRDEGQTWQSNYIIRDDGPVSDLGYPG
                     SVELPDGRIYTVYYQKPAKDV"
     gene            1260..2243
                     /locus_tag="ENN56_01955"
     CDS             1260..2243
                     /locus_tag="ENN56_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDS74283.1"
                     /translation="MQAQNSATPWGQLVLFAGRSIVSVAEAIAEELGIPLSDATVSQF
                     PDTESSVMLKENVRGADVFVIQSTGAPVNDNYMDLLIMIDAIRRASAARITVVMPYMG
                     YARQDRKATGREPISAKLMADLITAAGADRVVSVDLHNSAIQGFFNIPLDHLTAIPAL
                     AQELKNCTLVDPVLVSPDVGRAKMVEKFQQVFDIPLVMMHKRRKGLEVSVTEVIGDVK
                     DRTPILIDDIIASGSIIKHVDALVQRGARPEVYMAITHPVLVGDAVQRLQHPALKALI
                     TTDTLAIPSSKQSGKIRVVSLAPMLADVIKRIHLNQSVSEVFTKLFEDFPV"
     gene            complement(2325..4007)
                     /gene="groL"
                     /locus_tag="ENN56_01960"
     CDS             complement(2325..4007)
                     /gene="groL"
                     /locus_tag="ENN56_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDS74284.1"
                     /translation="MAAKIIAYDVKARAKILEGVNVLANAVKATLGPKGRNVVIDKSW
                     GAPTVTKDGVTVAKEIDLPDPFENMGAQMVKQVASKTSDVAGDGTTTATVLAQAIYSE
                     GLRNVTAGTNPMGIKRGIEKAVRAMVEQIRKQSVDISGREQIAQVATISANSDETIGN
                     LLADAMEKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYISPHFVTDQDTMEVEFDDA
                     VILIYDKKISAISDILPLLEQVGNATKQLLIIAEDVEGEALATLVVNKLRGTIQVAAV
                     KAPGFGDRRRAMLDDIAILTGGTVISEEAGLKLENVQMKDLGRARSVRIDKEDTTIVE
                     GAGSRDMISGRIKQIRQQIEATTSDYDREKLQERLAKLAGGVAVINVGAASEVEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVALLRARKALDALSGLTSEELVGVDIVRRAVAEP
                     AKQIAENAGFEGSVVVQKILSVDDANYGFNAQEEEYGDLVKQGVIDPTKVVRAALENA
                     SSVASLLLTTEAMVTEKESDEPEDPGHSHGGGGMPGMGGMGGMGGGMPGMGF"
     gene            complement(4122..4415)
                     /locus_tag="ENN56_01965"
     CDS             complement(4122..4415)
                     /locus_tag="ENN56_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDS74285.1"
                     /translation="MTIKPLGDRVLVQPIEETNQVQGGIIIPDTAKEKPQQGKVVAVG
                     PGKTTESGTVLPMDVAAGVTVLYGKYAGTEVKIDGEKYLIMSQNDILGIVENA"
BASE COUNT          842 a         1443 c         1291 g         1099 t
ORIGIN      
        1 cacgtccttc gccggcttct ggtagtagac ggtgtagatg cgtccgtcgg gcagttcaac
       61 cgaacccggg tagccaagat cgctcacggg accatcgtcg cggatgatat agttcgactg
      121 ccacgtctga ccttcgtcgc ggctgatcat gacgcggtgc ccaaacggtt cgcggcggta
      181 cccgtacacg catacgatcg cacccgacga atgacgaatc atgtgcggag gtgagccgta
      241 gacgcccgtg tgatgtgctt ccgtccacgt cttccagccg tcgtccgatt cactctgtag
      301 gagtgagaag ctctcgtatg ccttcgcgtc accgctgtgc tgatagcgaa tcatcgcaat
      361 gagccgtccc gacgttgtct cgaccacgtg cggttcgtgg aggttctttg ggctgatttc
      421 cggaggaagt ggtacgctgc caagctcctc ccacgtgtac ccatcgtcac ggctgattgc
      481 cgcacgcata tcaccgacat gctccatcgg gtcacccatg ccgggcatcc accactgctt
      541 gccgaagtag agcagatcgc ccgattcgag ccgtatcgga ccgtgcggcg tgtttaccgg
      601 tgcgcggata aagtcgctcc acgcctcccc atcgacactg gtacgcaccc acgacccgcg
      661 ccatttctca cgcactgagt ccggtatcgc gtcgatcgcg ttgcgccatt cgtcacgaat
      721 ctcctcaggc gcgttctcga cgtttgcgat gttcttcgtg tccgacgtga accacgttag
      781 gaggaccttc tcgccgccga ggctgatgat ccccgcgtca cggtcgtcga gcggcgtgtc
      841 gttgatcacg cggggacgcg accacgtggc gccactgtct tcgctcgtga acagtaccgt
      901 ctttccccat gggcagacgt gaccccaacg caagccggaa gcgatggcgt acatccgccc
      961 ggattccgtt cgtgcgacac tcggccatcc gaagtaaccg aacttttcac ccgtcaagtc
     1021 gcagatcgta ccgtgaattg catcaacgcg tattccttgg ggcatggtgt ctccttatgc
     1081 ttcttcaatg tcgtatcatt cgatagtggg taggagatgg taacgcggta cagagccgtg
     1141 tggaaccccg tcagggcgag acgctttgtt cgccgcattg actacgccgt cgaaacgtcc
     1201 catttttcgt atactacggc actcataccg aaccgtcgtt aaatcagggg attgccactg
     1261 tgcaagctca aaattccgct actccatggg gtcagctcgt tctcttcgca ggacgctcca
     1321 tcgtctccgt cgccgaagct atcgccgaag agcttggaat cccgctctcc gatgccacgg
     1381 tctcccagtt cccggatacc gagagctccg tcatgctcaa ggaaaacgtg cgcggagccg
     1441 atgtatttgt gattcagtcg accggcgcgc cggtgaacga caactacatg gatctgctca
     1501 tcatgatcga cgcgatacgg cgtgcgtcgg cggcgcgtat caccgtggtc atgccgtaca
     1561 tgggttacgc gcgacaagat cggaaagcca ctggtcgcga accgatctcc gcgaagctca
     1621 tggcggacct catcactgcg gccggtgcgg atcgcgtcgt ctctgtcgat ctgcacaatt
     1681 ccgccattca gggctttttc aacatcccac tcgatcatct caccgcgata ccggcccttg
     1741 cgcaagaact caagaactgc actctcgtcg atccggttct cgtttcaccc gacgtcgggc
     1801 gtgcgaagat ggtggagaag ttccagcagg tgttcgacat cccgctggtg atgatgcaca
     1861 agcgtcgcaa gggtctcgaa gtctccgtca ccgaagtcat tggcgacgtg aaggatcgca
     1921 cgccgatcct catcgacgac atcattgcgt cgggcagtat catcaaacac gtcgatgcgc
     1981 tcgtgcagcg tggcgcgcgg ccggaagttt atatggcaat tacgcacccg gtgctcgtcg
     2041 gagacgcggt gcagcggctt cagcatcccg cgctcaaagc gctgatcacg actgacacgc
     2101 tcgccattcc gtcgtcgaag cagtcgggga aaattcgcgt cgtgagcctc gcgccgatgc
     2161 tcgccgacgt catcaagcgg atacatctga atcagtccgt gagcgaagtc ttcacgaagc
     2221 tctttgaaga tttcccggtt tgattcgatc acctatcggt acatgaaaaa cgcctccgca
     2281 tcccttcgga tacggaggcg ttccgtatta acggagttgg gagcttagaa gcccatcccg
     2341 ggcattccgc cgcccattcc acccattcca cccatgccgg gcattccgcc accgccgtgg
     2401 ctgtggccgg gatcttccgg ctcgtccgat tccttctcag tcaccattgc ttcggtggtg
     2461 aggaggagac tcgcaacact cgatgcgttc tcaagcgccg cgcggacgac tttcgtcgga
     2521 tcgatcacgc cctgtttgac gaggtcgcca tactcttctt cctgcgcgtt gaagccgtag
     2581 ttcgcgtcat ccactgagag gatcttctgc acgacgacgc tgccttcgaa tccggcgttt
     2641 tcggcaatct gcttcgccgg ctcggcgacg gcgcgacgaa cgatatccac accgaccagt
     2701 tcctcggagg taaggccgct gagcgcatcg agcgctttcc gtgcgcggag cagcgcaacg
     2761 ccgccaccgg gcacgatgcc ttcttctacc gcggcacgag ttgcattcag cgcgtcttcg
     2821 acacgtgcct tcttttcctt catctcgact tcgctggcgg caccaacgtt gatcaccgcc
     2881 acgccaccgg ccaacttcgc aaggcgctcc tggagcttct cgcggtcgta gtcggacgtg
     2941 gtagcttcga tctgctggcg aatctgcttg atccgaccgc tgatcatgtc gcgtgaaccg
     3001 gcgccttcta cgatcgttgt gtcttctttg tcgatacgaa cgctccgcgc ccgcccgagg
     3061 tccttcatct ggacgttttc gagcttgagc ccggcttctt cgctgatgac ggttccgccg
     3121 gtgaggatcg cgatatcgtc gagcattgcc ctgcgccggt caccaaagcc cggagccttc
     3181 accgctgcaa cttgaatcgt gccgcgaagc ttgttcacga caagtgtcgc gagcgcttca
     3241 ccttcgacgt cctcggcgat gatgaggagc tgtttcgttg cgttaccgac ctgctcgaga
     3301 agcggcagga tgtcggaaat agcggagatt ttcttatcgt agatgaggat gacggcatcg
     3361 tcgaactcga cttccatcgt atcctgatca gtaacgaagt gcgggctgat gtatccgcgg
     3421 tcgaactgca taccttcgac gacgtcgagc gtcgtttcaa tactcttcgc ttcttcgacc
     3481 gtgattactc cgtccttacc gaccttctcc atcgcgtcgg cgagaagatt accgatcgtc
     3541 tcgtcgctat tcgccgaaat ggtcgcgacc tgcgcgatct gctcacgtcc ggatatatcc
     3601 accgactgtt tgcggatctg ctcgaccatg gcgcgcacgg ccttttcgat accgcgcttg
     3661 atgcccatgg ggttcgtgcc ggcggtgacg ttccggagtc cctctgagta gatcgcctga
     3721 gcgagaaccg ttgcggtcgt cgtgccgtcg ccggcaacgt ccgaggtctt cgaggcgacc
     3781 tgcttcacca tctgtgcacc catgttctca aatggatcgg gaaggtcgat ctcttttgcg
     3841 acagtgacgc cgtccttggt caccgtcggt gctccccacg atttgtcgat gacgacgttc
     3901 cggcctttgg ggccgagcgt cgcctttacc gcgttggcca agacgtttac gccttcgagt
     3961 attttcgctc gcgcttttac gtcataggcg atgattttcg ctgccatgcg tgtttgcctc
     4021 caatttcaga ttcgctgatg tcaacactga accgcgcacg tatcggtaat cgtcgtgcgt
     4081 gcacgtgtat gattttcctc tatggagagg ccgaaccggt atcacgcgtt ttcgacgata
     4141 ccgagaatgt cgttctggct catgatgaga tacttttcgc cgtcgatctt aacttcggtg
     4201 ccggcgtatt tcccgtagag caccgttact cctgcggcaa cgtccatcgg cagcacggtt
     4261 cccgattcgg tcgtcttacc gggtcccact gccacgactt ttccctgctg cggtttttcc
     4321 ttggcggtgt ccggaataat gatgccgccc tgtacctggt tggtttcttc gatcggctgc
     4381 accagaacac gatcaccaag tggtttgatc gtcatagttg cgcccttccg tgtgattgaa
     4441 acgctgtgtg agtgtactcg gcgcacagtt tggagtgttg actcggtatc gtgcgcgctc
     4501 ctcgattagc actcatcgtt cgagagtgct aataagaaga tagtatgtag cccggcgatg
     4561 tgtcaagttc aacaagcgcg aacgcgagcg tagcgaaggg ttcgcgctac gtgtcgcgtt
     4621 ccccaccctc ccgtttcacc gtctgatgct gcgtgagcag atcggttacc gtgcg
//
LOCUS       DSCC01000163            2210 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033610, whole genome shotgun sequence.
ACCESSION   DSCC01000163 DSCC01000000
VERSION     DSCC01000163.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033610"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            298..576
                     /locus_tag="ENN56_01970"
     CDS             298..576
                     /locus_tag="ENN56_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDS74286.1"
                     /translation="MTGNARAQIRIIGRVQGVGFRFFTVKHASRYTVTGWVRNRPDGS
                     VEVEAEGPRDRVEAFIEELRHGPSFAHIDDVDVEWVDPRGTSSFEVIG"
     gene            576..1088
                     /locus_tag="ENN56_01975"
     CDS             576..1088
                     /locus_tag="ENN56_01975"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_925693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDS74287.1"
                     /translation="MDFTKFIRDIPDFPAPGILFRDITPLLAHPEAFSEVIDELAIAV
                     SGYQIDKVAGIESRGFIFGTPLALKLGVGFVPIRKPGKLPADTIREEYTLEYGTNAIE
                     MHSDALEPGDRVLIVDDLLATGGTACAAAKLIERGGGKVAALAFVIELTFLEGRKKLE
                     GYEIVDLVTY"
     gene            1112..1642
                     /locus_tag="ENN56_01980"
     CDS             1112..1642
                     /locus_tag="ENN56_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74288.1"
                     /translation="MARTLIALVILAVLAVGCQIGQPDPDYYLARATTPEGKHWQVYF
                     RGAWRDISVDGMSEPTTLREHVFRGDNWFMFAGVIDTVELRASGPHLPVFMLEAWSII
                     WPVRRRLTIDGPLDTTGFQHPVEDDFIDKPAFQRYGINYAAQWWPLVVNIDYPEGEAR
                     ARGISLRRSLFDLESE"
     gene            complement(1643..2209)
                     /locus_tag="ENN56_01985"
     CDS             complement(1643..2209)
                     /locus_tag="ENN56_01985"
                     /inference="COORDINATES: protein motif:HMM:PF10050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HDS74289.1"
                     /translation="MDTDLSPFIVAAANDAGASRVIPIDPTGIRYYTEVRRLCERNAC
                     GVLGKNWQCPPYVGSVADTSSRAKRFACGVVVQSVGVLEDSYDIEGMGREQKAHQDRL
                     RAVGKRLRTEHPDVPFLLLGAGPCDVCPRCTVLDNEPCRFPDRIMPSMESYGMNVKEV
                     VESVGLPYISGKNTVTYSGLILYGSAEE"
BASE COUNT          455 a          683 c          633 g          439 t
ORIGIN      
        1 caccgggcga tcccgatcgg ttcatgcgac agcttcacac cagcctgagc gggggattac
       61 cggagacgat cgtctacggt cgcgacgggc tcccgcgtct catccttcgc ggcgagcaga
      121 cgtacgcgca gtttcgcgag tacgtagagc gtgcactgca gtagatcgct cagcacttcg
      181 ggcaggcgcg ggcatgtctc gcgcctgcca tgcaacgccg ttttcgcctt cgctcagtgc
      241 agcaatcctc cgtagagtac ccattaccgt aagcgggatt ggggggtacc ttcaaacatg
      301 acagggaatg ctcgcgcgca aatccgcata atcggtcgcg tgcaaggcgt cggatttcgc
      361 tttttcacgg taaaacacgc gagccgctac accgttaccg gatgggttcg caaccggccc
      421 gacggctctg tcgaagtgga agccgaaggg ccacgggacc gcgtggaagc gtttatcgaa
      481 gaactccgac atggcccgtc gtttgcccac atcgatgacg ttgacgtgga atgggtcgat
      541 ccacgtggca catcttcatt cgaggtaatc ggttaatgga ttttaccaag tttatccgtg
      601 acatacccga cttccccgct ccgggtatcc tctttcgcga catcacaccg ttactcgcac
      661 atcccgaggc attcagcgag gtgatcgacg agctcgcgat cgcggtttcg ggataccaga
      721 tcgacaaagt cgcgggtatc gaatcgcgcg gctttatctt cggcacgccg ctcgccctca
      781 agctcggtgt gggcttcgtc ccaattcgca aaccggggaa gcttcctgcg gataccattc
      841 gcgaagaata cacccttgaa tacgggacaa acgccatcga gatgcattcc gacgcgctgg
      901 aacccggcga ccgtgtgctg atcgtcgacg atctgctcgc gaccggtggg accgcgtgcg
      961 cggccgcgaa gcttatcgaa cgcggcggag ggaaagtcgc cgcgctcgcg tttgtcatcg
     1021 agctcacgtt tctcgaaggt cggaagaagc tcgaaggcta cgaaatcgtc gatctcgtga
     1081 cgtattgacc gctctggagc acatcaatcg aatggcacgt acgctcatcg cactcgtcat
     1141 actcgcggta ctcgctgtcg gatgtcagat cgggcaaccc gatcccgact actatctcgc
     1201 ccgcgcgacg actcccgagg ggaagcactg gcaggtctac tttcgcgggg catggcggga
     1261 catcagcgtt gacggaatga gcgaaccgac aacactccgg gagcatgtct tccgaggcga
     1321 caactggttc atgttcgccg gtgtgatcga tacggtggag ttacgcgcct ctgggccgca
     1381 cttgccggtc ttcatgctcg aagcgtggag catcatctgg ccggtgcgaa gacgccttac
     1441 gatcgacggg ccgctcgata ccacggggtt tcagcatcca gtcgaggacg acttcatcga
     1501 caaacccgcg ttccagcgtt acggcatcaa ttatgcggcg cagtggtggc cgctcgtggt
     1561 aaatatcgac tatccggaag gcgaggcccg cgcacgtggg atttcactca gacgtagcct
     1621 tttcgatctg gaatccgagt agtcactcct ccgcactgcc gtaaaggatg agtcccgaat
     1681 aggtcaccgt gttcttccca ctgatgtagg gaagcccaac cgattcgacg acctccttca
     1741 cgttcatccc atacgattcc atcgacggca tgatccggtc gggaaaccgg cacggctcgt
     1801 tgtcgagaac cgtgcagcgc ggacacacat cgcacggccc cgcaccgagg agcaggaatg
     1861 gcacatccgg gtgctccgtg cgtagtcgct ttccaacggc acgcaaacga tcctgatgtg
     1921 ccttttgctc gcgtcccatc ccctcgatat cgtacgaatc ctccaggacg ccgaccgact
     1981 gcacaacgac tccgcaagca aaccgcttcg cccgcgacga ggtatcggca acgctaccga
     2041 catacggagg gcattgccag ttcttcccaa gcacgccgca cgcatttcgt tcgcaaagac
     2101 ggcgcacttc agtgtaatac cggatgcccg tcggatcgat gggaattacc cgcgatgcgc
     2161 ccgcgtcgtt cgccgccgcg acaatgaacg gagaaagatc ggtatctatc
//
LOCUS       DSCC01000164            1748 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046189, whole genome shotgun sequence.
ACCESSION   DSCC01000164 DSCC01000000
VERSION     DSCC01000164.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046189"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(249..923)
                     /locus_tag="ENN56_01990"
     CDS             complement(249..923)
                     /locus_tag="ENN56_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDS74290.1"
                     /translation="MSMSTDERHPAISVDLVVLTILDDALHVLLVKRRNEPFAGCWSL
                     PGGRAHIEESLEDAARRVLNEKTGISRIFLEQLYTFGEPNRDPRQRIITIAYYALIPF
                     DTTTVLHAGHAEQDIAWAPVATLGCLAFDHEMIIEATLQRLRNKLDYTPVGLELLPEK
                     FTLSELQRVYEIILGKTIDKRNFRKKIRGKDWIERTEGFRRDGPHRPAQLYRFVPRST
                     PNGSYL"
     gene            1170..>1748
                     /locus_tag="ENN56_01995"
     CDS             1170..>1748
                     /locus_tag="ENN56_01995"
                     /inference="COORDINATES: protein motif:HMM:PF12708.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74291.1"
                     /translation="MKGTEMVEEPSGTIDVKRFGARGDGRIDDTNAIQHAIDACAESG
                     GGVVFLPTGRYRIDGALELKRGVIIRGSGGAVDGWGMPTVLEAYNRDRSVITATWQSG
                     VQYLEIYYPEQRLEPDGLIPYPWSIYGSGENVSIIGVSLRNSYDGINLTAACRHLVRD
                     VHGSPLHTGLYVDQCYDVGRIENVHYWPFNGPE"
BASE COUNT          363 a          451 c          547 g          387 t
ORIGIN      
        1 gcagaaggac tcgatattcc aactgcggga ctcatgtacg cggcactgaa gggtatcaag
       61 gtgcagccgt acacgaagat ggaagcgaaa ccgatttacg gtggtccgtc cggagtcacg
      121 atccctgacg agaccaccgc ccgatttgca cgggaaatgg atgcgttgcg cgcgaagtat
      181 cggtgagttc ggcggacact gttacgaaac gcggcggaac ggcgcatcgc gttccgccgc
      241 gctgcgtttt aaagatagga accgttcggc gtacttcgtg gaacaaaccg gtaaagttgg
      301 gcgggacgat gcggaccgtc acggcgaaaa ccttcggtgc gctcgatcca atccttaccg
      361 cgaatttttt tgcggaaatt ccgtttatct atggtcttcc caaggatgat ctcgtacacc
      421 cgctgaagct cgctcagcgt gaacttctcg gggagtagct ccagaccgac gggcgtgtaa
      481 tcgagcttgt tacggaggcg ctgcaaggtc gcttcgatga tcatctcgtg atcgaacgca
      541 aggcacccta acgtggcgac tggtgcccag gcgatgtctt gttccgcatg accggcgtgc
      601 agcacggttg tggtatcgaa cggaatcagc gcgtaatacg cgatcgtgat gatgcgttgc
      661 cggggatcac gattcggttc accgaacgtg tatagctgct caaggaagat ccgcgagatg
      721 ccggttttct cgttgagaac gcggcgagcg gcatcttcga gcgactcctc gatgtgagcg
      781 cgtccgcccg gaagggacca gcagccggcg aaaggttcat ttcgccgttt cacgaggagc
      841 acgtgcagcg cgtcgtcgag gatcgtcaga acgacgaggt cgacactgat ggctgggtgt
      901 cgttcgtcgg ttgacatcga catcgagtca ggcatgcgtt gtgcgctcca tggtttatct
      961 cgccccgtgt ttttgggcgc gtgtgcgggt gtttcgtgtg attccttcgt tacgctaaag
     1021 ataccgcgac gggacttagt gtgtcaagca cacgaacccc gactgcgccg tcaagcgatg
     1081 tacgatcccg gtactatccc attggttctc ttctcgggct gttgttccat cgaagaggac
     1141 cttgctttgt ttccacgatt actggatagg tgaaaggtac ggagatggtc gaagaacctt
     1201 cgggaacgat cgatgtgaaa cggttcggcg cgcgtggtga cggacgaata gacgatacga
     1261 atgccataca gcacgcgata gacgcgtgcg cggaatccgg tggtggcgtc gtgtttcttc
     1321 cgactggacg gtaccgtatc gacggagcgc tggagttgaa acgcggagtg attatccgtg
     1381 gctccggcgg cgcggtagat gggtggggca tgccgacggt gctcgaagca tacaaccgcg
     1441 atcgatccgt cattacggcg acgtggcaga gcggcgtgca gtacctcgaa atctactatc
     1501 cggaacagcg actcgagcct gatgggctga ttccctatcc gtggtccatc tacggtagcg
     1561 gtgaaaatgt atcgatcatc ggcgtatcac tacggaactc gtacgacggg atcaacttga
     1621 cggccgcgtg tcggcatctt gttcgcgatg tgcacgggag tccgcttcat acgggcttat
     1681 atgtcgatca atgctacgac gtcggtcgca ttgagaacgt gcactattgg ccgttcaatg
     1741 gaccagaa
//
LOCUS       DSCC01000165            2014 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038109, whole genome shotgun sequence.
ACCESSION   DSCC01000165 DSCC01000000
VERSION     DSCC01000165.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038109"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..333)
                     /locus_tag="ENN56_02000"
     CDS             complement(<1..333)
                     /locus_tag="ENN56_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74292.1"
                     /translation="MTGPYWCQGTRIAVVTGQNAPLSERYVAEVAARSIRERGVESAV
                     FVGVDSITGSWDLTLYLGTSAAHPWLADRFRERDRLRFTSPLRRGAEASLGMTFRYEG
                     ANCAGIVAE"
     gene            579..>2014
                     /locus_tag="ENN56_02005"
     CDS             579..>2014
                     /locus_tag="ENN56_02005"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDS74293.1"
                     /translation="MTEQKHIRNFSIIAHIDHGKSTLADRLLETTGTLVSSQMQEQVL
                     DSMDLERERGITIKAHAIRMKYLACDGITYELNLIDTPGHVDFSYEVSRSLAACEGAI
                     LIVDAAQGVEAQTVSNLYHALEADLEIIPIINKIDLPSAQVDMVREQLRDMIGCTDEE
                     ILLTSAKTGVGVPEVLEAIVHRVPPPIGNPDAPLRALIFDSVYDQYQGAVIYVRVVDG
                     ELRAGQRIRLAAADIEYEIDEVGYLKLGRQRVDRITTGEVGYVTAAIKSIEDARVGDT
                     VMDAENLATSALKGYKEVKPMLYAGIYPIAPEDYEDLRDALERLKLNDASLVYEPESS
                     LALGFGFRCGFLGLLHMEIVQERLDREFDLDIITTVPNVRYRVVTTDGKETWVENPSN
                     LPPTVKIERIEEPYLTVQIVTPTEYIGPIMKLCTERRGDYKTTEYLSQRTVDIRFELP
                     LSEIVFDFYDKLKSGTRGYASFDYEFSE"
BASE COUNT          496 a          539 c          543 g          436 t
ORIGIN      
        1 ctcagccact atgccggcgc aatttgcgcc ttcgtatcgg aaggtcattc caaggctcgc
       61 ttccgctccg cgtcgaagcg gcgaagtgaa acgcaagcgg tcgcgctccc gaaaacggtc
      121 tgcgagccat ggatgtgcgg cggacgttcc aaggtagagc gtaagatccc acgatcccgt
      181 aatcgaatcg acgcctacga aaaccgcaga ctctacgccg cgctcgcgta tggaccgagc
      241 ggcaacctcc gcgacatacc gttcgctgag cggggcgttc tgtccagtca cgacggctat
      301 tcgggtgcct tgacaccaat atggtccggt cacggtaatc cctttcgcgg taatttctca
      361 ccgattcgat ggaacacaga cgaaaatcga aaagtagccg tggcgcaacg ggttcgcgtc
      421 gtaatcgaga ggctgataga ctcgtctgtt gcttgaatcg cgcatatgcg gtattcttat
      481 ttgtttacac cggcatacgc aaccgccgat gaaacgacgc tcgcacgact acgtgttccc
      541 gcgattgaaa ccgcgctacg gcagaggttc gccaacgaat gaccgaacaa aaacacatac
      601 ggaatttctc gataatcgca catatcgacc atgggaaatc gactcttgcg gaccggttgc
      661 tcgagactac cggcacactc gtttcgagtc agatgcaaga acaagttctc gatagcatgg
      721 acctcgaacg tgaacgagga ataaccatca aagcccatgc gattcgaatg aaatacctag
      781 cgtgcgatgg gatcacgtac gagctgaatc tcatcgatac accggggcac gtagacttct
      841 cgtacgaggt ttcgcggagt ctcgctgcat gtgaaggtgc gattctcatc gtggatgcgg
      901 cccagggagt cgaagcgcag accgtatcga atctgtatca cgcactcgaa gcggaccttg
      961 agataatccc aatcatcaac aagatcgatt taccgagtgc gcaagtggat atggtcaggg
     1021 aacaacttcg cgatatgatc ggctgtaccg acgaggaaat ccttctcacg agtgcaaaaa
     1081 ccggtgttgg cgtgcctgaa gttctcgaag cgatcgttca ccgcgttcca ccaccgattg
     1141 gtaatcccga tgcgccgcta cgcgcactca ttttcgattc tgtctacgac cagtaccaag
     1201 gtgcggtgat ctacgtacga gttgtcgacg gtgaattgcg cgccggtcaa cgcattcgcc
     1261 ttgctgcagc tgatatcgag tatgaaatcg acgaggttgg gtatctgaaa ctcggacgtc
     1321 aacgtgttga tcgtatcaca acgggtgaag tcggatacgt tacggctgcg atcaaatcga
     1381 tagaagatgc gcgtgtcggc gacacggtca tggatgcgga aaacctcgcc acatcggcgc
     1441 tcaaggggta caaagaagtc aagccgatgc tctacgcggg aatctatccc atcgcaccgg
     1501 aagactacga ggatcttcgc gatgcgcttg agcgcctgaa actcaatgac gcttcgctcg
     1561 tctacgaacc agagagttca ctcgctcttg gctttggatt ccgttgtgga tttctgggcc
     1621 tcctccacat ggaaatcgtg caggaacggc tcgaccgtga gtttgatctg gacatcatca
     1681 ccacggtacc gaacgttcgg tatcgcgttg tgaccaccga tggcaaggaa acgtgggtcg
     1741 agaatccgtc gaaccttccg ccaaccgtga aaattgagcg tatcgaagag ccgtatctca
     1801 cagtacagat tgtgaccccg acggagtata tcgggccgat tatgaaactc tgtaccgaga
     1861 ggcgcggtga ctataagaca acggaatacc tttcacagcg aaccgtcgat atcagattcg
     1921 agttgccgtt aagcgagatc gtattcgatt tctatgacaa gctcaagtcg ggaacgcgtg
     1981 gctatgcgtc gttcgattac gaattctccg aata
//
LOCUS       DSCC01000166            7191 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1006623, whole genome shotgun sequence.
ACCESSION   DSCC01000166 DSCC01000000
VERSION     DSCC01000166.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1006623"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..682)
                     /locus_tag="ENN56_02010"
     CDS             complement(<1..682)
                     /locus_tag="ENN56_02010"
                     /inference="COORDINATES: protein motif:HMM:PF04561.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDS74294.1"
                     /translation="MPERIIERRSFAKLPTIVDVPNLLEVQVQSFEEFLQANKSADKR
                     EPAGLQAVFESIFPIADIHNLYSLEFVNYTVGRPKYTIDECRERGATYSAPLRSTLRL
                     IAREKPEKGKDEAQPKIKDVIEQQVYLGDLPMMTEQGTFVINGSERVIVSQLHRSPGV
                     FFDETVHPNGKRLFTSRIIPYRGSWVEFSMDINDIMYVHIDRRRKLPVTVLLRALGFS
                     SDLDVLNLF"
     gene            complement(849..1235)
                     /locus_tag="ENN56_02015"
     CDS             complement(849..1235)
                     /locus_tag="ENN56_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002825717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDS74295.1"
                     /translation="MADLAALKEQLSQLTVLEIAQLTKELEEAWGVSAAAPVAVAAAG
                     GAAGGDAAAAAEEEQTEFDVILKDVGAQKIQVIKVVRELTGLGLKEAKAVVDGAPSPV
                     REAVSKDEAAQAKEKLEAVGATIEIK"
     gene            complement(1273..1818)
                     /locus_tag="ENN56_02020"
     CDS             complement(1273..1818)
                     /locus_tag="ENN56_02020"
                     /inference="COORDINATES: protein motif:HMM:PRK00099.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDS74296.1"
                     /translation="MPTPKKDQTIVEITEVFKTANGVFLTDFTGLSVEHMTELRKRCH
                     AEKVGFRVVKNTLAQRAAEAAGMPDISEWLTGSTAIAYTDDATRALKLLQKFASDVRE
                     ANGKPEVKTGVVDQALLSAAEMKMLAELPSVEVVKAKFLGLLNAPAQQFVFLLNAAPS
                     QFARLLGAYQKTLEEGNSAEA"
     gene            complement(1878..2582)
                     /locus_tag="ENN56_02025"
     CDS             complement(1878..2582)
                     /locus_tag="ENN56_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDS74297.1"
                     /translation="MAKNGKKYVGDFDKVDREREYSLHDAVAILKSFEPRKFDQTVDS
                     AVRLNVDPRKADQMVRGSVTLPNGTGRTRRVLVFARGDAADAAREAGADFVGAEDLVE
                     KIQGGWTDFDVAIATPDMMGLVGRLGRVLGPRGLMPNPKTGTVTPETAKAVDDAKGGK
                     VDFRVDRAGNAHAPLGLISFEPEKIEENARTLFEALMRQRPAAIKGIYVRSATISATM
                     TPGVKIDRGELLNTLR"
     gene            complement(2595..3035)
                     /gene="rplK"
                     /locus_tag="ENN56_02030"
     CDS             complement(2595..3035)
                     /gene="rplK"
                     /locus_tag="ENN56_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDS74298.1"
                     /translation="MAKKVVAQIKIQLPAGSATPGPPVGPALGAHGLNIMEFVKAFNE
                     RTREQAGMTIPAVITVFADRSFTFITKQPPVVALLKKFAGIPKGSGTPNTQKIGRVTW
                     DQCIEIAKIKLPDMNTAKIESAASMVAGTARSMGLDVVGRPAQK"
     gene            complement(3174..3755)
                     /gene="nusG"
                     /locus_tag="ENN56_02035"
     CDS             complement(3174..3755)
                     /gene="nusG"
                     /locus_tag="ENN56_02035"
                     /inference="COORDINATES: protein motif:HMM:PRK05609.1-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDS74299.1"
                     /translation="MTDTPQEQPTRKLRWYAVHTLSGHENKVCHYLKNSAEALALGDS
                     IGDCVVPTEEVAEMRQGKRIMTKRKFLPGYVLVQMEMNSEARHVVANSPSVTGFVGVR
                     SDQEPQPLRPSEVERILGQIERKTTAPETDSAYRVGDRVKVIDGPFSEFIGVIEATDH
                     AKDKVTIAVSIFGRPTKVELDALQVEPAPEQQS"
     gene            complement(3757..3897)
                     /gene="secE"
                     /locus_tag="ENN56_02040"
     CDS             complement(3757..3897)
                     /gene="secE"
                     /locus_tag="ENN56_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDS74300.1"
                     /translation="MGKVNWPSRDELKTSTIIVLVVTIIFAVFIGAFDWVLSQIVQWF
                     LG"
     gene            complement(4007..4084)
                     /locus_tag="ENN56_02045"
     tRNA            complement(4007..4084)
                     /locus_tag="ENN56_02045"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(4047..4049),aa:Trp,seq:cca)
     gene            complement(4111..4263)
                     /gene="rpmG"
                     /locus_tag="ENN56_02050"
     CDS             complement(4111..4263)
                     /gene="rpmG"
                     /locus_tag="ENN56_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDS74301.1"
                     /translation="MPRDIIVLACEECKRRNYSTTKNRRTHSERVTFKKYCPFDRKHT
                     PHRETR"
     gene            complement(4315..5505)
                     /gene="tuf"
                     /locus_tag="ENN56_02055"
     CDS             complement(4315..5505)
                     /gene="tuf"
                     /locus_tag="ENN56_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDS74302.1"
                     /translation="MAKAKFERTKPHVNVGTIGHVDHGKTTLTAAITQVLANRGLAKA
                     QAFDEIDNAPEERARGVTINVHHTEYESANRHYAHVDCPGHADFVKNMITGAAQMDGA
                     VLVVSAADGPMPQTREHILLARQVNVPYIVVFLNKVDMVDDEELLDLVELEVRELLSA
                     YEFPGDDIPVIRGSALQALENPTDETASAPIMELVETMDSYIPQPEREVDRPFLMPIE
                     DVFSITGRGTVGTGRVERGIVKVGDEVEVVGVRDTRKTVVTGLEMFRKLLDEARAGDN
                     AGALLRGVNKGDLERGMVLAKPGTITPHTKFEAEVYVLSKDEGGRHTPFFPGYRPQFF
                     FRTTDVTGSIQLAEGTEMVMPGDHTTMIVELITPIAMDEGLRFAIREGGRTVGAGSVT
                     KIIQ"
     gene            complement(5603..5678)
                     /locus_tag="ENN56_02060"
     tRNA            complement(5603..5678)
                     /locus_tag="ENN56_02060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5643..5645),aa:Thr,seq:ggt)
     gene            complement(5745..5820)
                     /locus_tag="ENN56_02065"
     tRNA            complement(5745..5820)
                     /locus_tag="ENN56_02065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5785..5787),aa:Gly,seq:tcc)
     gene            complement(5870..5956)
                     /locus_tag="ENN56_02070"
     tRNA            complement(5870..5956)
                     /locus_tag="ENN56_02070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5919..5921),aa:Tyr,seq:gta)
     gene            complement(6054..6129)
                     /locus_tag="ENN56_02075"
     tRNA            complement(6054..6129)
                     /locus_tag="ENN56_02075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(6094..6096),aa:Thr,seq:tgt)
     gene            complement(6340..>7191)
                     /gene="ligA"
                     /locus_tag="ENN56_02080"
     CDS             complement(6340..>7191)
                     /gene="ligA"
                     /locus_tag="ENN56_02080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00533.24,HMM:PF03119.14,HMM:PF14520.4"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDS74303.1"
                     /translation="IPELRPANATPFQFPTRCPVCESVLHRDEGDAIVRCDNAACPAQ
                     VRGRLIHWGSRGAMDIDGLGEAIVDQLVERGLATNIADLYDLTWEPISTLDRMAEKSA
                     RNLVTAIEKSKQRPLSALIFGLGIRNVGATTARSLAELFRSVETLMAATEDDLVAVTD
                     IGPTVARSIVDYFGVPENRELVARLAAHGLLTEEAAPAEPNPSASTPFAGKTVVLTGA
                     LTQYSRDDAGEIIRRLGGKVTGSVSKNTDVVIVGADAGSKRTKAEQLGVEIWDEARFL
                     REIDGAT"
BASE COUNT         1511 a         2261 c         1871 g         1548 t
ORIGIN      
        1 agaagagatt caatacgtca agatcggagc tgaagccaag cgcacgcaaa agtaccgtga
       61 cagggagttt gcgacggcgg tcgatatgga catacatgat gtcgttgatg tccatggaga
      121 actcgaccca gcttccgcga tacggaatga tgcgcgacgt gaatagacgc tttccgttcg
      181 ggtgtactgt ctcgtcgaag aagacacccg gggaacggtg aagctgactc acaatgacgc
      241 gctcggaacc attgatcacg aacgtgcctt gctcggtcat catcggcaaa tcgccaagat
      301 agacctgttg ttcaatcacg tccttgatct tcggttgcgc ttcgtctttc cctttttcgg
      361 gcttttcgcg cgcaatcagc cgaagcgtcg aacgcaatgg cgcggaatat gtcgcaccac
      421 gctcacggca ctcatcgatt gtgtatttcg gtcgcccgac cgtgtagttg acgaattcga
      481 gcgaatagag gttgtgtatg tcggcgatcg ggaatatgct ttcaaagacg gcctgaagtc
      541 ccgccggctc ccgcttgtcg gcagacttgt tcgcttgcag gaactcctcg aaggattgaa
      601 cctgtacttc gagaagattc ggcacgtcga cgatggtcgg gagcttggcg aaagaccggc
      661 gttcgattat ccgctctggc atggcctgct atcgctcctt ttggctgtgt gccaacagat
      721 atgtacaaat cacccgtaac cgcggtggac cgggcgttaa atacgaaaac gagacacaca
      781 aagacgacgc agtctgccac cacgaaggcc gcagacgcat cgtctttgcg taagtccagt
      841 caatccaact acttgatttc gatggtggcg ccgacggcct cgagcttctc tttggcctgt
      901 gcggcttcgt ccttcgatac ggcttccctg accgggctcg gtgcaccatc gacgacggcc
      961 ttcgcttcct tgagacccaa gccggtcagc tcgcgcacga ccttgatgac ctgaatcttc
     1021 tgagcgccga catccttgag gatgacatcg aattcggtct gctcttcttc cgcagctgcg
     1081 gcggcatcac caccagcggc tccaccggcg gctgctacgg caaccggcgc ggcggcggat
     1141 acgccccaag cttcttcaag ctctttcgtc aactgtgcga tctcaagcac ggtgagctgg
     1201 ctgagttgtt ctttgagtgc agcaagatct gccatggaac caaaacctcc ttgaacaccc
     1261 gggggcatct cgttacgcct ctgccgagtt tccttcttcc aacgtcttct ggtacgcccc
     1321 gagcaggcgt gcgaactgcg acggcgccgc attcaacagg aacacaaact gctgagccgg
     1381 cgcgttgagc agcccgagga acttggcctt tacgacctcc accgagggca actccgccag
     1441 catcttcatc tctgccgcgc tgagcagagc ctggtctact acaccagtct tgacctccgg
     1501 cttcccattc gcttcacgaa cgtcgctggc gaatttctgc aagagcttga gcgcacgcgt
     1561 tgcatcgtcg gtataggcaa tcgccgtcga gccggtgagc cactcgctga tatccggcat
     1621 acccgccgct tccgccgcac gttgtgccaa tgtgttcttc acgacgcgaa atccgacctt
     1681 ttccgcatga caccgcttcc gcaactcggt catatgttcg accgagagtc cggtaaagtc
     1741 ggtaaggaaa acaccgttcg ccgtcttgaa gacttcggtg atctctacaa tcgtttgatc
     1801 ttttttgggt gtcggcatcg ccttcgccct tctgaatggt cagtcccgct ccccgttcga
     1861 aacgggaagc agaatcacta ccgaagtgta ttgagtagtt cgccccggtc gatcttcaca
     1921 ccgggtgtca tcgtggcgct gatcgttgcc gaacgaacgt agataccttt gatcgccgcc
     1981 ggcctctgcc gcatgagcgc ttcaaataac gtccgcgcgt tttcttcgat cttctccggt
     2041 tcgaaggaga tcaaaccgag tggtgcgtga gcgttacccg cgcggtcgac gcggaagtca
     2101 accttaccac ccttcgcgtc gtcgacggcc ttcgccgtct caggcgtcac cgttccggtc
     2161 tttggattcg gcatcaaccc acgcggtccg agcacgcgac cgagtctacc gacgagaccc
     2221 atcatgtccg gtgtcgcgat cgccacgtcg aagtccgtcc aaccgccttg aatcttctca
     2281 acgagatcct cggcaccgac gaaatccgct ccggcttcgc gagctgcgtc cgcggcgtcg
     2341 ccacgtgcga atacaagcac acgacgcgta cgacctgtgc cgttcggaag cgttaccgat
     2401 ccgcgcacca tctgatccgc tttccgcgga tcgacgttca agcgcactgc actgtcaacc
     2461 gtctgatcga acttacgcgg ctcgaagctc ttgagaatcg caacggcatc gtgcagagag
     2521 tattcgcgtt cgcgatcgac cttgtcgaag tcgcctacgt acttttttcc attcttcgcc
     2581 attgttccga tcctctactt ctgcgcaggg cgaccgacaa cgtcgaggcc catgctccga
     2641 gcggtacccg cgaccatact cgccgcagac tcaatcttcg cggtgttcat gtcaggcaat
     2701 ttgatcttgg cgatctcaat acactgatcc cacgttaccc gaccgatctt ctgagtattt
     2761 ggagtgcctg atcccttcgg gattccggca aactttttca gaagggctac caccggcggc
     2821 tgcttcgtga tgaacgtgaa gctccggtcg gcaaacaccg tgattaccgc cggaatggtc
     2881 atgccggcct gttcacgcgt acgctcgtta aacgctttga cgaactccat gatgttgagc
     2941 ccgtgtgcac caagcgccgg cccaacaggc ggacccggcg ttgccgaacc cgctggcaac
     3001 tgaatcttga tctgggcgac aacctttttc gccacggtct atcctccgac gccctccacc
     3061 tcgcaatatc gcagaggtca ggctatcgag tgaaacaacg gaatcacacg cgaacgccca
     3121 cgaagtcgcg ggcgtcgatg attcctgcta cagtgtactc acattccatg caatcaggac
     3181 tgttgctcgg gcgccggttc tacctgcaac gcgtcgagct ccaccttcgt gggacgaccg
     3241 aaaatactca ccgcgatggt gactttgtcc ttcgcatggt ccgttgcctc gataacgccg
     3301 ataaactcgc tgaacggacc atcgataacc ttcacgcggt cacctacgcg atacgccgaa
     3361 tcggtttcag gcgcagtggt tttccgttcg atctgaccga gaatccgttc aacttcgctg
     3421 gggcgcagcg gctgcggctc ctggtcggaa cggactccaa cgaatccggt cacgctcggc
     3481 gaattcgcca ccacgtgccg cgcctcgctg ttcatttcca tctgaacgag cacgtacccg
     3541 ggcaaaaact tccgcttcgt catgatacgc tttccctgac gcatctcggc gacttcttca
     3601 gtcggaacga cgcaatcacc gatcgaatcg ccgagcgcga gcgcttcggc cgagttcttc
     3661 aggtagtgac acaccttgtt ttcgtgaccc gaaagggtgt gcaccgcgta ccagcgcagt
     3721 ttacgggtgg gctgttcttg aggcgtatcc gtcatattat ccgaggaacc attgtacgat
     3781 ttgactcaat acccagtcga acgcaccaat gaagaccgcg aaaataatgg tcaccacaag
     3841 tacgataatg gtcgatgtct tcaattcgtc tcgcgacggc caattgactt tgcccatctc
     3901 cgagacgact tcatggtagt agttcacgat cttctgacgc acgaggaaca cctcaagctc
     3961 gaacggcggt gcgacacttc attgacgcac acggtgaact acgaactggc aggagtggag
     4021 ggattcgaac ccccgaccgt cggatttgga gtccgatgct ctagccagac tgagctacac
     4081 tcctaccgtt cagttatcga tacgcgcgcg ttaccgcgtc tcccgatggg gagtatgctt
     4141 gcggtcaaac gggcagtact tcttaaatgt cacccgctcc gagtgcgtac gacgattctt
     4201 cgtcgtcgaa taattccgac gtttgcactc ttcacatgcc aatacaatga tgtcgcgagg
     4261 catgacggtg aaacctttcc aatagtgccc gaagttgcta tcgagcaact ggtattactg
     4321 aataatcttg gtaacggagc cggcgccgac ggtgcgtccg ccttcacgaa ttgcgaaacg
     4381 cagaccctcg tccatggcga tcggcgtgat caactcgacg atcatcgtcg tgtgatcacc
     4441 aggcatcacc atctccgttc cttccgccaa ctgaatcgaa cccgtcacat ccgtcgtacg
     4501 gaagaagaac tgaggacgat accccgggaa gaacggcgtg tgacgaccac cttcatcctt
     4561 cgacaacaca tacacctcgg cctcgaactt cgtgtgcggc gtaatcgtac caggcttcgc
     4621 caacaccatg ccacgctcga gatcgccctt gttcacacca cgaagcagcg caccggcgtt
     4681 gtcacctgca cgggcttcgt caagaagctt ccggaacatc tcaagaccgg tcacaaccgt
     4741 cttgcgcgta tcacgaactc cgacaacctc aacttcgtcg ccaaccttca caataccacg
     4801 ctcaacacga ccagtgccta ccgtgccgcg acccgttatc gagaacacgt cctcgatcgg
     4861 catcaaaaac ggacggtcga cctctcgctc aggctgcgga atgtacgagt ccatcgtctc
     4921 gaccaactcc atgatcggcg ccgaagccgt ctcatccgtc ggattctcaa gcgcctgaag
     4981 cgccgaacca cggatcaccg gaatgtcatc gcccgggaac tcatacgccg acaacagctc
     5041 gcgtacctca agctccacga ggtcgagaag ctcttcgtcg tccaccatgt caaccttgtt
     5101 caggaagaca acgatgtacg gcacgttcac ctgacgtgcg agaagaatgt gctcgcgcgt
     5161 ctgcggcatc ggaccgtcgg ccgctgatac taccagcacc gcaccatcca tctgcgccgc
     5221 acccgtgatc atgttcttca cgaagtcggc gtgacccgga cagtctacgt gcgcgtagtg
     5281 acgattcgcc gactcatact ccgtgtggtg aacgttgatc gtcactccac gggcacgctc
     5341 ttccggcgca ttatcgatct cgtcaaacgc ctgagccttc gcaagaccac gattcgccaa
     5401 cacctgcgtg atcgccgccg tcaacgtcgt cttaccgtgg tcgacgtgac caatcgtccc
     5461 cacgtttacg tgcggcttgg tcctctcaaa ttttgccttg gccatgagag tctcctctga
     5521 tttctcgttc gttacgccaa aaagcgcgaa ggtcccgcct tcacgccctc cgctccggga
     5581 acgcgtcggt tccatgaaca tctggagccc gggatcggac ttgaaccgac gaccccgtcc
     5641 ttaccaagga cgtgctctac cggctgagct acccgggctt gattctataa tcacgggcca
     5701 gtcaacagcc gtcccgatca aattcacgcg aaacacgcga aaaatggagc gggaaacggg
     5761 gctcgaaccc gcgacgatca gcttggaagg ctgatgctct accaactgag ctattcccgc
     5821 tcggttgtgc cgtcaaaccc gaaatggccg atttgcagcc gttatcgtat ggtgggcgtc
     5881 gagggattcg aaccccctta ggcgtcagcc aacggattta cagtccgccc cggctctccc
     5941 actccggcgc acgcccgttc caccaaatgt atcgagtctc tgggatcggt gccgctgtca
     6001 tatcgccgag cgcgtcgcgc ccgaaccata cacattgtct caacaacagc cgatgaagcc
     6061 gatggaggga atcgaaccca cgacctgctc attacaagtg agccgctcta ccaactgagc
     6121 tacatcggca gtacatgaga ctctcaatgt taccacgagc tcgcgaattt gtcaagggta
     6181 aatagaaaaa ttacgggctg cgtcgggtgt cgtcaactcc gattccgaaa gcggtccggg
     6241 tcatgaatac gcgcgccgtg gtagatcccg taaatatcgg cgaacgggct gcaagcttaa
     6301 gaagataggt ataacgtggt gtatcgcaaa gtgtctgcgt caggtcgcgc cgtcaatctc
     6361 gcgaaggaaa cgcgcctcat cccagatctc gactcccagt tgctcagctt tcgtgcgctt
     6421 cgatccggcg tcagctccga cgatgacaac gtccgtattt ttcgacacgc ttccagtgac
     6481 ctttccgccg agtcgtcgaa taatctcgcc cgcgtcatct cgactgtact gcgtaagcgc
     6541 gcccgtcagc accactgtct tcccggcaaa cggtgtcgat gcagatggat tcggctcagc
     6601 cggcgcggcc tcttcggtca gcaatccgtg cgcagcgagt cgcgcgacga gttcacgatt
     6661 ttcgggcacg ccgaagtagt ccacaatgct ccgtgcgaca gttggtccaa tatcggtcac
     6721 cgcgacgagg tcatcctccg tcgccgccat gagcgtctca acgctacgga aaagttcggc
     6781 aagcgaccgg gcagtcgtcg caccgacatt ccggatgcca agcccgaata tcagcgcact
     6841 gagtgggcgc tgcttcgatt tctcgatcgc ggtgacgaga ttccgcgccg atttctccgc
     6901 catgcgatcg agcgtgctga tcggttccca cgtcagatcg tagagatcag cgatgttcgt
     6961 tgccagtccg cgctccacaa gctgatccac aatcgcctcg ccgagcccgt caatgtccat
     7021 cgcgccacgc gagccccaat gaattagccg cccacggact tgcgccgggc aggcagcatt
     7081 atcacaacga acgatcgcgt ctccttcatc tcggtgaagt acagactcgc agaccgggca
     7141 acgcgtggga aactggaacg gcgtagcgtt cgccggacga agctcgggga t
//
LOCUS       DSCC01000167            7190 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1006626, whole genome shotgun sequence.
ACCESSION   DSCC01000167 DSCC01000000
VERSION     DSCC01000167.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1006626"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..409
                     /locus_tag="ENN56_02085"
     CDS             <1..409
                     /locus_tag="ENN56_02085"
                     /inference="COORDINATES: protein motif:HMM:PF01808.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HDS74304.1"
                     /translation="FAYVAVKHTRSNAIILARAYKPGCYQLVGLGAGQPNRVDSLRAL
                     AAPKARANFEMEYDELSPGGTKDIYVEEQFRRVVLASDAFFPFDDTVREAHAAGIRYI
                     VQPGGSIRDDEVIATANELGIAMAFTGRRHFMH"
     gene            464..1483
                     /locus_tag="ENN56_02090"
     CDS             464..1483
                     /locus_tag="ENN56_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDS74305.1"
                     /translation="MKFSLAGMMSNDIGVDLGTANTLIWVKGRGVVLNEPSIVAVDHT
                     THRPLAIGLEAKEMLGRAPGDIEVIRPMKDGVIANFEVAEEMLRGFLTKVQRNRGLFR
                     PRVVICVPSGITEVEKRAVRDSAEAARANEVHLISEPMAAAIGVGLPIERPVGSMIID
                     IGGGTSEIAVMSLSGIVTTTSERIGGDEMDDAIINYVKRTYNLSIGHRMAEEIKVKIG
                     SAYPLENELETTVRGRHLVTNIPQVVVVKSEEIREALAESIDRIINGVLQTLERTPPE
                     LAADIIDRGIFLTGGGALIKGLDDRLKEATNLPINIVEDPLTAVVRGTGAVLEDVVRY
                     RKVLF"
     gene            1572..2414
                     /gene="mreC"
                     /locus_tag="ENN56_02095"
     CDS             1572..2414
                     /gene="mreC"
                     /locus_tag="ENN56_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDS74306.1"
                     /translation="MLQRVAQWLWVHLTGVVLAFTLISSLFFLTRAPSLKVGPVRWAL
                     LYAISGGQSVFRYPIHIVSLKTENRYLRSQLLLKVINETELRELRAENERLREMIAFT
                     RDRDLELLAARVLSHDVEIPPTSIVIDVGTSAGVAPFDAVVSARGLVGRCDESPTWNR
                     SIVRLLTDPGIRTSVMVQNPARPMGILRWNGAEMHIDNIAQELPVAVGETILTSGLGG
                     IFPPGLHVGVVDSVREDPHALFKDVIMKPSARLYRLEEVFVVRVHRDSASIGQQEGHN
                     AVGR"
     gene            2398..2901
                     /gene="mreD"
                     /locus_tag="ENN56_02100"
     CDS             2398..2901
                     /gene="mreD"
                     /locus_tag="ENN56_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDS74307.1"
                     /translation="MRWVGEALWLISAAIVYAVYGPIIAINSAVPVLTTIVIVRVALT
                     GGPLAGNIMGFAGGLLLDVSTPEWFGAAMFVDSIIGFVVGIIRDRIVLDSPFARFVVL
                     LVAATTHSLGIVVMRSFVTPDMAPEPFINAFASGLYTAVLGGMWWILCSVLRALVGLR
                     SVWHVER"
     gene            2888..4702
                     /gene="mrdA"
                     /locus_tag="ENN56_02105"
     CDS             2888..4702
                     /gene="mrdA"
                     /locus_tag="ENN56_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDS74308.1"
                     /translation="MLNGSDSAGNERRRTSVVMIAIVVGFLVLGVQLYRLQIQQHDDY
                     ARRSEQNAMRTVPISAVRGRILDRSGNIIVGNRPAYTLGVIPAEVADAAKLDSILSNE
                     IGLASGHLIDRVRSNRRRHSPIELRRDAPYATIAYLEEHRHKLPSVLYMVESRRRYPY
                     GQIGAHFLGYVREIGQTQLMSISDESYALGDLMGQTGIEAEYERFLRGTKGEEYQEVT
                     VRGQVIHTARREPVRGSDVRLSIDLDLQLTAEAAMDTIKRGALVAMDPKTGEVLAMVS
                     RPTFDPAVFSFVVPTSVWRVLNDNMERPMFNRAAMGTYPPGSTIKMITAIAGLDSGVI
                     TPTTLLNPCRGSLRYGDRTFLCWRESGHGAVNVVGAIERSCNVFFYQLGDLIGVDKWG
                     EIARKFGFGERTGLDLPAEDGGIVASSDVYNRRMGRFGWTRGEALNVAIGQGITLVTP
                     LQMARYTCALATGKLPKPHLALGLIEADGTERPLPVPPPEPLDINLELLPIIREGMIL
                     VTEGDHGTARRSRVPGYHVAGKTGTAQNPHGDDHSWYVGYVPADDPVIAIAVIAENAG
                     HGSDIAAPIAGRVMRRFMLRDKSRSQPSAEAAPIVERR"
     gene            4706..5938
                     /gene="rodA"
                     /locus_tag="ENN56_02110"
     CDS             4706..5938
                     /gene="rodA"
                     /locus_tag="ENN56_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDS74309.1"
                     /translation="MSITTRNVRELIDWPLAIATLALIAIGIVGISSATGEQAASRVQ
                     GLATIQAVWATLAVSVAIATLFLPVRFWHAMAYVIYGVSIAALVATLFAGSLGMGAER
                     WLQIGPMRVQPSEFGKLALILALSRFLSGPRIRINRFWDLSRALALVIVPVVLIRQQP
                     DLGTALVYLAVALPMVFFAGLRNLYLFLILSPIVNAICAFAGWIPWLAFCLVFVGLLF
                     LTKPSTMWTVVLITVNLGIGIVAPIFWDELSPYQQRRIETFFNPENDPLGAGYQIIQS
                     KVAIGSGGMWGKGFQQGTQTQLAFIPETHTDFIFAVLAEEFGFVGAIVVIGLLLFAIW
                     RIIGLANTLNNRFASFICIGVGTLIAFHALVNIGMTVGLMPVTGLPLPFVSYGGSAML
                     TDGYAIGLVLSLGLRRFE"
     gene            5958..6929
                     /locus_tag="ENN56_02115"
     CDS             5958..6929
                     /locus_tag="ENN56_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74310.1"
                     /translation="MSDRLRSDMMAFTLNVFVIAVVVFLPVSTSAEPTLRVFYTANVK
                     GFIDPCGCPGAPAGGITRRATFFRERADVPSIKLDGGEILGNDTPIDRMRTIYLLRAM
                     QEMGYRTIGVGPRDFMYGREFIEFIAQEFQFTLVNANVVDENGAPIFAPYAIEEVSET
                     GLLGMQRSVTRIGILNVIGEDRTPLTIPTNPPVYVTDPVTAASRIVDELRSEVDLIVL
                     MANVNPETLNTLTQIDGVDIILMTRLTRIPGEYVVVNDKTAIGYSTIEGRGIGQIDVT
                     LRSGELVDVKGSLTMLFDRIPEDRTMLRIREEFENWKDQYIDELPSP"
BASE COUNT         1574 a         1923 c         2013 g         1680 t
ORIGIN      
        1 attcgcctac gtagccgtca aacatacgcg ttcgaatgcg atcatccttg cacgagccta
       61 caaacctggt tgctaccagc ttgtcggtct cggtgcggga cagccaaatc gcgtcgacag
      121 cctccgcgcg cttgccgcac cgaaagctcg cgcgaacttc gagatggagt acgacgagtt
      181 gtcacccggc ggcacaaagg acatctacgt agaagagcaa ttcagacgag tcgttcttgc
      241 gtcagatgcg ttcttcccgt ttgacgacac ggttcgcgaa gcccatgcgg ctggcatccg
      301 gtacattgta caaccaggcg gttcaatccg agacgacgaa gtgatcgcga cagcaaacga
      361 acttggcatc gcgatggcgt ttacgggtcg tcgtcatttc atgcactaac gttacattct
      421 acatagaatc gagcggtaag cggcggaacc gcgagggggt acaatgaagt tctcacttgc
      481 cgggatgatg tctaacgaca tcggtgttga tctcggcacg gcgaatacgc tgatttgggt
      541 caaaggtaga ggtgttgtcc ttaatgagcc gtcgattgtt gcggttgatc atacgacaca
      601 tcgtccgctt gcgatcggac ttgaagcgaa ggagatgctc ggccgcgcac ccggcgatat
      661 cgaagtgatt cgcccgatga aggacggtgt gatcgcgaac ttcgaggtcg ccgaggagat
      721 gcttcgtgga ttcctcacga aggttcaacg gaatcgcggg ctctttcgac cgcgtgtggt
      781 gatctgtgtc ccgtctggta tcacggaagt tgagaaacgt gcggtacgcg attccgccga
      841 ggcggcccgt gcgaacgagg tgcaccttat cagtgagccg atggccgctg cgatcggcgt
      901 cggtcttccc attgaacgac ctgtcggttc gatgattatc gatatcggtg gcggcacgag
      961 tgaaatcgcc gtgatgagtc tttcaggcat cgtcacaacg acgtccgagc gcatcggcgg
     1021 cgatgaaatg gacgacgcca tcatcaacta cgtcaaacga acctataacc tctccatcgg
     1081 tcaccgtatg gccgaagaga tcaaggtcaa aatcggctcg gcgtatccac tcgaaaacga
     1141 actggaaaca acagtgcggg gtcgacacct cgtaacgaac atcccgcaag tggtcgtggt
     1201 gaaatccgaa gagattcgcg aagcgctcgc agagtcgatt gacaggatta tcaacggcgt
     1261 gcttcagacg ctcgaacgga cgccaccgga acttgccgcc gatattatcg accgaggtat
     1321 attcctgacc ggcggcggag cgcttatcaa aggactcgat gatcgactca aggaagcgac
     1381 gaacttgcca atcaacatcg tcgaagaccc gcttacggca gtcgtacgtg gcaccggtgc
     1441 agtgctcgaa gacgtagtac ggtatcgcaa agtgctcttc taaagagagt caagctgtaa
     1501 atcgatgtgt agagattcct accgcacccg cggcggagat acgccgtgac gtttgaaggt
     1561 attgccgcac aatgctacag agagttgccc agtggttgtg ggtccacctt acgggagtcg
     1621 tactcgcttt tacgctgatt tcctcgctct ttttcctcac acgcgcccca tctctcaaag
     1681 ttggtcccgt tcgctgggcg cttctctatg caatctccgg tggccagagc gtttttcgat
     1741 acccaattca tattgtttcg ctcaaaacgg aaaaccgtta tctccgaagc cagcttctgt
     1801 tgaaggtgat aaacgaaaca gagttacgcg aacttcgcgc ggaaaacgaa cgactgcgtg
     1861 agatgatcgc cttcacgcga gaccgcgact tagaactgct cgctgcacgc gtgctttcgc
     1921 atgatgttga aatcccgccg acaagcatcg ttattgatgt cggtacctcc gccggcgtcg
     1981 ccccattcga cgccgttgtg agcgcgcgag gtctcgtcgg acggtgcgac gaatctccga
     2041 cgtggaatcg atccatcgtt cgcctgctta ctgatccggg catccgaacg agtgttatgg
     2101 tacaaaatcc cgcacgaccg atgggtatcc tgcgatggaa cggtgcggaa atgcacatcg
     2161 acaacatcgc tcaggagctt cctgtcgccg tcggggaaac catactcacg agtggcctcg
     2221 gtggcatctt tcctcccgga ctccatgttg gcgtcgtcga ctccgtacgc gaggacccgc
     2281 atgcgctctt caaagacgtt atcatgaagc cgagtgcacg attataccgc ctcgaagaag
     2341 tcttcgtggt gagagttcac cgagactccg catccattgg tcagcaggag ggacacaatg
     2401 cggtgggtcg gtgaagcgct ctggctcata tcagccgcga tcgtgtacgc cgtctacggt
     2461 cccatcatcg caatcaatag cgccgtacct gttctaacaa cgatcgtgat cgttcgtgtt
     2521 gcgcttacgg gcggtccgct tgcaggtaac atcatgggat tcgccggcgg tctcctgctt
     2581 gatgtctcta cacctgaatg gtttggtgcg gcgatgtttg tcgattcgat aattggattc
     2641 gtcgtgggaa tcatacgcga tcgaatcgtt ctcgatagtc cgtttgcgcg attcgtcgtc
     2701 cttctcgtcg ccgcaacgac ccattcgctc ggaattgtgg ttatgcgaag tttcgtgacg
     2761 ccggacatgg cgccggaacc tttcatcaac gcattcgcgt ccggtttgta tacagccgtc
     2821 ctcggcggaa tgtggtggat actgtgtagc gtgcttcggg cgctggtcgg tttgcggagt
     2881 gtttggcatg ttgaacggta gtgactccgc gggaaacgag cgccgacgta ccagcgtcgt
     2941 catgatcgcg atcgttgtcg ggttcctcgt gttgggcgtg caactctatc gtcttcagat
     3001 acagcaacac gatgactacg cccgacgttc agagcaaaat gcgatgcgca ccgttccgat
     3061 ctcagcggtt agaggtcgga tacttgatag aagtggcaat atcattgtcg gcaaccgtcc
     3121 cgcgtacacc ctcggtgtca tacctgccga agttgcggac gcagcgaaac ttgacagcat
     3181 attgagtaac gagatcggac ttgcgtccgg acacctcatc gatagggtcc ggagtaatcg
     3241 acgacgtcat agtccgattg aactccgcag agacgcgccg tatgcaacga tcgcgtatct
     3301 cgaagaacac cgtcacaaac tcccttcggt tctctacatg gttgagtcgc gacggcgata
     3361 tccgtacggc cagatcggcg ctcacttcct gggttacgtc cgcgagatcg ggcagactca
     3421 actcatgtcg atttccgatg aatcatacgc gcttggcgat ctgatgggtc agaccggtat
     3481 cgaagcggag tacgaacggt ttctccgagg aacgaagggg gaggagtatc aggaagtcac
     3541 tgttcgcgga caggtgatcc atacggcacg acgtgaacca gtccgcggct cggatgtgcg
     3601 actcagcatc gacctcgatc tacagctcac cgcggaagcg gccatggaca cgataaaacg
     3661 tggtgcactc gtagccatgg atccgaagac cggcgaagtg ctcgcgatgg tgagtcgtcc
     3721 gacgttcgat cccgcagtgt tctcatttgt cgtgccgacc agtgtttggc gcgtattgaa
     3781 cgacaatatg gaacgtccga tgttcaatcg tgccgctatg ggaacctatc ctccgggctc
     3841 tacaataaag atgattacgg cgattgccgg tctcgatagc ggcgtgatta cgcctacgac
     3901 attactcaat ccgtgccgtg gttcacttcg atacggtgac agaacattcc tctgttggcg
     3961 cgaatcaggc catggtgcgg taaatgtcgt aggcgcaatc gaacgaagct gcaacgtgtt
     4021 tttttaccaa ctcggtgatt tgattggtgt cgataagtgg ggagaaatcg cacgtaaatt
     4081 tgggtttgga gaaagaaccg ggctcgacct tcctgcggag gacggtggta ttgtcgcgag
     4141 ttccgatgtg tacaaccgac gaatgggccg cttcggatgg acacgcggcg aagcgctcaa
     4201 cgtcgccatt ggtcagggca tcacccttgt cacaccgctc caaatggcac gttacacatg
     4261 cgcacttgcg actggtaagc ttccgaaacc tcacctcgcg ttgggtctca ttgaagccga
     4321 tggcaccgag cggccactgc cagtcccacc accggaacca ctcgatatca atctcgaact
     4381 tcttccgatt attcgggagg gaatgattct cgtcacagaa ggcgatcacg gaactgcacg
     4441 ccgttctcgc gtacccggat atcatgtcgc agggaaaacc ggcacggcgc aaaatccgca
     4501 cggagacgat cattcgtggt atgttggata tgtgcctgcc gatgacccgg taatcgcgat
     4561 cgccgtgatc gccgaaaacg ccgggcacgg tagtgatatc gctgcaccga tcgccggtcg
     4621 ggtaatgcga cggtttatgt tgcgagataa atcgcgatcc caaccgtcgg ctgaggctgc
     4681 gccgatcgtt gagcggagat aaccgatgtc gataacgacg cgaaatgtgc gcgaactgat
     4741 agactggccg ctcgcgattg caactttggc actcatcgcg attggtattg tcggcatatc
     4801 aagcgcaact ggcgaacagg cagcgagtcg cgttcaagga cttgccacca ttcaagccgt
     4861 ttgggcgacg cttgccgtct cagtagcgat cgcgacgttg tttctcccag ttcgattttg
     4921 gcacgcgatg gcgtatgtga tctatggcgt ttcgatcgcc gctctcgttg ctacactctt
     4981 cgctggatcc ctcggtatgg gtgcggagcg ttggttacag atcggtccga tgcgcgtcca
     5041 accgtcggaa tttgggaagc tcgcactgat cctcgcgttg agtcgttttc tcagcggacc
     5101 gagaatacgg atcaatcggt tctgggacct ctctcgtgca ttggcgctcg tgattgttcc
     5161 ggtagtgctg attcgccagc aaccggatct cggaactgcg ctggtgtatc tcgccgtagc
     5221 tcttccgatg gtcttcttcg ccggtcttcg aaacctgtac cttttcttga tcctatcccc
     5281 aatcgtaaac gctatctgcg cgtttgccgg ctggattcct tggctcgcgt tctgcctcgt
     5341 attcgtgggt ttgctcttcc tgacgaaacc gtcgacgatg tggaccgtcg tattgattac
     5401 cgtcaatctc ggcatcggga tcgtcgctcc gattttttgg gacgagctgt ctccgtacca
     5461 acaacgtcga atcgaaacgt tcttcaatcc ggaaaacgat cctcttggcg ccggatatca
     5521 gatcatccag tcgaaagttg caatcggttc cggaggaatg tggggtaaag gttttcagca
     5581 gggaactcaa acgcaactcg cattcattcc cgaaactcat accgacttca tatttgccgt
     5641 cctcgccgaa gagttcggat ttgttggtgc aattgtagta attggattgt tactattcgc
     5701 tatatggcgg ataattggat tggcgaacac actgaataac cgattcgctt cattcatctg
     5761 tatcggtgtc ggtacactca ttgcctttca tgcgctcgtc aatattggta tgacggtagg
     5821 cttgatgccg gtcacgggtc tcccgcttcc gtttgtgagt tacggcggct ccgcaatgct
     5881 tacggatgga tatgctatcg gtctcgtgct cagtctcggg cttcgccgat tcgagtagca
     5941 aatgaccgga gagatgtatg tcggatagat tacgaagtga tatgatggcg tttacgctga
     6001 atgtattcgt aatcgcagtc gtcgttttcc tgccggtttc gacgagcgcg gagccgacat
     6061 tacgggtatt ctacacagca aacgtaaaag gttttattga tccgtgcgga tgccccgggg
     6121 ctcctgccgg tggtatcaca cgacgcgcga cgttctttcg agagcgcgcc gacgtaccct
     6181 cgatcaaact tgacggtggc gagatcctcg gtaacgatac gccaatcgac cgcatgcgaa
     6241 cgatatacct acttcgcgcg atgcaagaga tggggtaccg gacgatcggc gtcggtccgc
     6301 gcgatttcat gtacggacga gagttcatcg agttcattgc acaagagttt caattcacgt
     6361 tggtaaacgc aaacgttgtc gacgagaacg gtgccccgat tttcgcgccg tatgccatcg
     6421 aggaagtctc cgaaaccgga ctgttgggta tgcagcgttc cgtgacccga attggtattc
     6481 tgaacgtgat cggagaagat cgtacaccgc taaccatacc aaccaatcct ccggtatacg
     6541 taaccgatcc cgttaccgca gcgagcagga ttgttgatga actccggagc gaagtcgacc
     6601 tcatcgtgct gatggccaac gtaaacccgg aaactttgaa cacactaaca cagatcgatg
     6661 gtgtggacat catactcatg acgcgcctta cccgaattcc gggagaatac gttgtcgtca
     6721 acgataagac ggcaatcgga tactcaacaa ttgaaggaag aggcattggc cagattgatg
     6781 ttacgctgag aagcggcgaa ttggtcgatg tcaaagggtc gctaacgatg ctcttcgatc
     6841 ggattccgga agacagaaca atgctacgaa tccgagaaga atttgagaac tggaaagacc
     6901 aatacatcga cgagcttccc agtccgtgaa atacgtcaga ttggaggtat caatgcgacg
     6961 ttgggttagc atagcgtttg ctgcgctcat cgcgatggcc gctttcgtga ccacgacgca
     7021 gtcctcgtct ccagcaaaat ccattacgtt gtttcttact gccactgcta caggtaatct
     7081 cgaaccgtgt ggatgtccgg caaacccaag cggcggagtg tcgcgccgtg ccgcgtatat
     7141 tcgtgaccac actcgttccg atgcacctcc ggtgctgctc gacggtgggg
//
LOCUS       DSCC01000168            1721 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047219, whole genome shotgun sequence.
ACCESSION   DSCC01000168 DSCC01000000
VERSION     DSCC01000168.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047219"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..582)
                     /locus_tag="ENN56_02120"
     CDS             complement(<1..582)
                     /locus_tag="ENN56_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDS74311.1"
                     /translation="MDIERIRKELTRLDLDGWLLYDFQGINPIAREIAGLTETFLTRR
                     WFCLIRREGSPTWLVSRIERGQFANVQGEVRAYLSWSDLQQQLNDVLGGCKAVAMEYA
                     PDGAIPYISRVDGGTLEMVAAADIELVSSADLIQWCQARWSTQQLATHMEASRHLNTA
                     RELAFRFIGESIRDAHRITEYDVQQKILEFFAQN"
     gene            complement(666..1277)
                     /locus_tag="ENN56_02125"
     CDS             complement(666..1277)
                     /locus_tag="ENN56_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74312.1"
                     /translation="MKTADTFVFNEYTHVAVRGLPFGNGNDNDVRGTGSEHAMDQEMR
                     RDILRRYIRTAMTRDSRLSRYIAISWPVDDAESATEVAINGRMPGEIVLSVRSAAITN
                     EGAQHLTDAGFAAEYDERNPRYRVFQRSAARAAIENLVDFAEWVFLVVLGAPSDYTPT
                     VIPAGSSESGKAKPGCGKSCLTVMKVAFWIMLGVIVLLTWVLR"
     gene            1325..1645
                     /locus_tag="ENN56_02130"
     CDS             1325..1645
                     /locus_tag="ENN56_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74313.1"
                     /translation="MPTLPEVTDSGLRPNTWHLSLLATKLGALRAAGMKVVMLSYLPD
                     WKRELDCRTEDVVGMIEYLVETKHPLMIGFGLDMHLIDAIMQCRLRRQGLYSREEWTG
                     IPDR"
BASE COUNT          351 a          498 c          499 g          373 t
ORIGIN      
        1 gttttgcgcg aagaactcga gaatcttctg ttggacgtcg tattcggtaa tccgatgggc
       61 gtctcggatg ctttcaccga tgaaccgaaa cgccagttca cgcgccgtat tcaggtgacg
      121 gctcgcctcc atgtgcgttg cgagctgctg cgtggaccac ctcgcttgac accactggat
      181 aagatcggcg ctcgacacga gttcgatgtc ggcggcggct accatttcca gtgtgccgcc
      241 atcgacacgg gagatgtacg gtattgcgcc gtcgggggcg tattccattg cgacggcttt
      301 acatccgccg agcacgtcgt tgagctgctg ctggagatcg ctccaactca ggtacgcgcg
      361 gacttcgccc tgcacgttcg cgaactgtcc gcgttcgatg cggctcacca gccacgtcgg
      421 tgaaccttcg cggcgaatca agcagaacca gcgtcgcgtc agaaatgtct cggtgagacc
      481 ggcgatttcg cgcgcaatcg ggttgatgcc ctggaaatcg taaagcagcc atccgtcgag
      541 atcgagccgc gtcagctctt ttcgtatccg ttcgatatcc atctgtctac cgttgaaaga
      601 ggatcgttca ccggaccatg atggtaccga tggtttccac gccgagcaac gcagcggtgt
      661 tcacgtcaac gtagcaccca tgtaaggagg acgattacgc cgagcatgat ccaaaacgcg
      721 accttcatca ccgtcaagca ggatttcccg cacccgggct tcgcttttcc cgactcactc
      781 gagccggctg gaataaccgt cggagtgtag tccgatggtg cgccgaggac aacgagaaac
      841 acccactcgg cgaagtcgac gaggttttcg atcgccgccc gcgccgcgct gcgttgaaaa
      901 accctgtatc ggggattgcg ctcgtcatac tcagcggcaa atccggcatc agtcaagtgc
      961 tgagcgcctt cgttggtgat cgcggctgag cgaaccgaca gcacgatttc cccgggcatc
     1021 cgaccgttga tagcgacttc ggtggcggat tctgcgtcat cgaccggcca tgaaattgcg
     1081 atgtaccggg atagccggga atcgcgcgtc atagcggttc gaatgtagcg tcggagaatg
     1141 tcacgacgca tctcctgatc catcgcgtgc tcgcttccgg ttcctctcac gtcgttgtcg
     1201 ttaccgttac cgaagggtaa gcccctgacg gcaacgtgtg tatactcatt gaatacgaac
     1261 gtgtctgcgg ttttcacaag cccgcgccgt atattgcccg gagtcatcga gaaggaatac
     1321 tgccgtgcct acgttgcccg aagtcaccga ttctgggtta cgcccaaaca cctggcacct
     1381 gagccttctc gcgacaaaac tcggtgcgtt acgcgccgcc ggtatgaagg tggtcatgct
     1441 gtcgtatctg ccggattgga agcgcgaact cgactgtcgc accgaggatg tggtcggaat
     1501 gatcgagtac cttgtcgaaa cgaaacatcc gctcatgatc gggttcggac tcgatatgca
     1561 tctcatcgac gcgatcatgc agtgtcgtct ccgcaggcaa gggctgtaca gccgcgagga
     1621 atggaccgga atccctgatc gatagggata cgtgtcgcgg gaagcgggag gatggattca
     1681 catatgattc tgctcgtcga tgacgaagcg ctccttcgag a
//
LOCUS       DSCC01000169            1666 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1049370, whole genome shotgun sequence.
ACCESSION   DSCC01000169 DSCC01000000
VERSION     DSCC01000169.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1049370"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(27..1082)
                     /locus_tag="ENN56_02135"
     CDS             complement(27..1082)
                     /locus_tag="ENN56_02135"
                     /inference="COORDINATES: protein motif:HMM:PF09084.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74314.1"
                     /translation="MNRITPNLRTVVALIVALAASFVLTGCSADPDDQGLTEINFLLD
                     WKPQMEQAGFVVALEKGYYREAGLKVNFLEGQGATTTAALVGTGQYPLGIASGGATII
                     ARARGAKPVSVALINQRSPTVIFALASSGIRTPSDLVGRSIALTKSGVKYDEYRALMA
                     KLGIDRSSINEVDVRKAVVPSLMSGSVDAALGYTEDQPVLVELQGHEVIRIPIHRYGV
                     NILSTNIIAHEDFISAQPDIVRRFVKASLKGWKRAIEYPDEAVMIYAARYPESNEDFV
                     RENFRQFRPILFSSATDTLGLGAQTLEGFRETERTLRELGIIDQHVNPEHAFTNDFLP
                     RIFVDNPEVEDLDVTRR"
     gene            complement(1140..>1666)
                     /locus_tag="ENN56_02140"
     CDS             complement(1140..>1666)
                     /locus_tag="ENN56_02140"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS74315.1"
                     /translation="AAVVMSRSAALNRTFYPLVVASQTFPKEALAPLFIVLIGFGILP
                     KILIAAMISFFPVTINTARGLESADPMDEDLFRSIAASKWQTFIKLELPSAVPFIFAG
                     LKMSATLSVIGAVVGEFVGASRGLGYLTQKGIDELQTELMFASLIVLGCMGVLLFSIV
                     EIVERFGFRKYATQ"
BASE COUNT          321 a          480 c          517 g          348 t
ORIGIN      
        1 ggtttgcacc atccccgcga taacgatcag cgccgcgtaa cgtcgagatc ttcgacctcg
       61 gggttgtcga cgaatatccg gggcaggaag tcgttcgtaa acgcgtgctc gggattcacg
      121 tgctgatcga tgatgccgag ttcgcggagc gtgcgttcgg tctcgcggaa accttcgagt
      181 gtctgcgcgc cgagaccgag tgtgtcggta gctgagctga agagtatcgg tcggaactgc
      241 cgaaaattct cgcgtacgaa gtcctcattg ctctccggat accgcgccgc gtagatcatg
      301 acggcctcat cggggtactc gatcgcgcgc ttccagccct tcaagctcgc cttgacgaat
      361 cgacgcacga tatccggttg cgcggagatg aagtcttcgt gtgcgatgat attcgtgctg
      421 aggatgttga cgccgtatcg gtgtatcggg atgcgaatta cctcgtgtcc ttgtagttcg
      481 acgagcaccg gctgatcctc ggtgtatccg agggctgcgt ccacgctgcc gctcatgagt
      541 gacggcacga cggctttccg cacgtcgact tcgttgatcg acgaccggtc gatgccgagc
      601 tttgccatga gcgcgcggta ttcgtcgtat tttacgccgc tcttcgtcag tgcgatggat
      661 cgcccaacca aatcggacgg cgtgcggatt cctgacgacg cgagtgcgaa gatcaccgtc
      721 ggtgagcgct ggttgatgag cgcgacggat acgggtttcg cgccgcgtgc gcgcgcgatg
      781 atggtcgcgc cgccacttgc gatgccgagc gggtattgcc ctgtgccgac gagcgccgca
      841 gtggtggtcg cgccctgccc ttcgaggaaa ttcaccttca agccggcttc acgatagtag
      901 cctttctcca gtgcgacgac aaacccggcc tgctccatct gcggcttcca gtcgagcagg
      961 aagttgatct cagtcaggcc ttgatcgtca ggatccgcgc tgcatcccgt cagcacgaac
     1021 gaagccgcga gggcgacaat caacgccact accgtgcgca gattcggtgt gattctgttc
     1081 acgtgattcc cccagaaaac gtagatggcg tgagcgcgtt cacgtcgata tcatgatcac
     1141 tactgcgtcg cgtacttccg gaacccgaag cgctcgacga tttccacgat gctgaagagc
     1201 agcacgccca tacatccgag cacgatgagc gatgcgaaca tgagttccgt ctggagttcg
     1261 tcgatgcctt tttgcgtgag gtagccgagt ccgcgactcg ctccgacgaa ttcaccgacc
     1321 accgcgccga tgacgctgag tgtcgcggac attttcaagc ctgcgaaaat aaacggcacg
     1381 gcgctgggga gctccagctt gatgaacgtt tgccacttcg acgcggcaat cgagcggaag
     1441 aggtcctcgt ccatcggatc ggccgattcg agtccgcgcg cggtgttgat ggtgaccggg
     1501 aagaagctga tcatcgcggc gatgaggatt ttcgggagaa tcccaaaacc gatcagtacg
     1561 atgaacagcg gcgcgagcgc ttccttcggg aatgtctgcg aggcgacgac gagcgggtag
     1621 aacgtgcgat ttagcgcggc ggaacggctc atgacgactg ccgcga
//
LOCUS       DSCC01000170            1617 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1051427, whole genome shotgun sequence.
ACCESSION   DSCC01000170 DSCC01000000
VERSION     DSCC01000170.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1051427"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..>1617
                     /locus_tag="ENN56_02145"
     CDS             <1..>1617
                     /locus_tag="ENN56_02145"
                     /inference="COORDINATES: protein motif:HMM:PF16656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74316.1"
                     /translation="PPTIELQYTGSMIIAEGEPFQMELFVTDFDMVEIMPSVEATVSP
                     SIEFDFVREGDWAMFSWTPGYDVAGAGQSTAFTVTFTATDEFSAQTVETLQLIVLNTN
                     REPSIEPIEPVTVTEGKQLVIPVDASDPDGDPLMLHVEPMPATATTDGLVFTWKTTQA
                     DVGEHVLMFSVWDREVFLRPSREANMPMYASTEVFVTVIDTNYAPVLQAIGDVQLNPG
                     DKLELFARATDPDGDAITFSDNSALFDIDPETGAISYTAVAGDAGTHLITITATDSKS
                     ASHSQTFYLSIIADTTPPVYVSMPIVTSTTTTSATIEWTTNEPSTSVVTYGLITGPRN
                     QTASDPALVTNHRVTITGLNPGVTYGFGTRSADAAGNLSVERTGSFTTRQLPPPPLTF
                     SATGPFVSNRGAQTATIEWETNRTATSVVQYGTVSATLNQTATGASGATHSVLLSGLT
                     AQTTYYYRAVSVDAGGESIQSDVRSFTTKALVVAPVIISGPTVIARTHETVTLQWDTD
                     RPATSIVQYGPTSAYGLRVERDLGDAVVSRH"
BASE COUNT          347 a          506 c          462 g          302 t
ORIGIN      
        1 ccaccgacaa tcgaactcca gtacaccggc tcgatgatca tcgccgaggg cgaaccgttc
       61 caaatggaac tcttcgtaac cgatttcgac atggtcgaga taatgccgtc agtcgaggct
      121 acggtgtcgc ccagtatcga attcgatttt gtgcgagaag gcgactgggc gatgttctca
      181 tggacgcccg gctacgatgt tgccggtgcg ggacaatcca ccgcgtttac ggtcaccttc
      241 accgcgaccg atgagttcag cgcgcaaacg gtcgaaacgc tgcaactgat tgttctcaac
      301 acaaaccgcg aaccgagcat cgagccaatc gagccggtca cggtaaccga agggaagcag
      361 ctcgttatcc cggtcgatgc gagcgatccg gacggcgacc cactcatgct acatgtcgaa
      421 ccgatgcctg cgaccgccac aacggacggt ctcgtgttta cgtggaaaac cacgcaagcc
      481 gatgtcggtg agcatgtgtt gatgttcagc gtgtgggacc gggaggtatt cctccgcccg
      541 tcacgcgaag cgaacatgcc gatgtacgca tcgacggaag tcttcgtgac agtcatcgat
      601 accaactacg cgccggtgct tcaggcgatc ggcgacgtgc aactcaaccc gggcgacaaa
      661 cttgagctat tcgcacgcgc caccgacccc gacggcgacg cgattacctt tagcgacaac
      721 tcggcgctct tcgacatcga cccggagaca ggtgcgattt cgtataccgc cgtcgccggt
      781 gacgccggaa cgcatctcat cacgattacc gccacggata gcaagagcgc ttcgcactcg
      841 cagacgtttt acctgtcgat cattgccgat acgacgccgc cggtctacgt gtcgatgccg
      901 atcgttacca gcacgacgac caccagcgcg acgatcgagt ggacgaccaa cgagccttca
      961 acgtcggttg ttacctacgg actcataacc ggtccgcgta accaaacggc aagcgacccg
     1021 gcactcgtta cgaaccatcg cgtaacgatt accggcttga atccgggagt gacctacgga
     1081 ttcggcacgc gctccgccga tgctgcggga aacctttcgg tggaacgcac cggctcgttc
     1141 acaacacgcc aacttccgcc gccgccgttg acgttctccg cgaccggtcc gttcgtatcg
     1201 aaccgtggcg cgcagacggc gacgattgag tgggaaacaa accgcaccgc aacaagcgtg
     1261 gtccagtacg gcaccgtgtc ggcgacgttg aatcagaccg ccaccggtgc aagtggagcg
     1321 acgcactcgg ttctgcttag cggactcacc gcacagacga cgtattacta tcgcgcggta
     1381 tcagtcgatg cgggcggcga gtcgattcag agcgacgttc gctccttcac gacgaaggcg
     1441 ttggtcgtcg caccggtgat tatcagtggc ccaacggtca ttgcgcgcac gcatgagacg
     1501 gttacccttc agtgggatac cgaccgtccg gcgacgagta tcgtgcagta tggtccgact
     1561 tccgcatatg gtctgcgtgt cgagcgtgat ctcggtgacg cggtcgtcag ccgccac
//
LOCUS       DSCC01000171            1910 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1041001, whole genome shotgun sequence.
ACCESSION   DSCC01000171 DSCC01000000
VERSION     DSCC01000171.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1041001"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..254)
                     /gene="tsaE"
                     /locus_tag="ENN56_02150"
     CDS             complement(<1..254)
                     /gene="tsaE"
                     /locus_tag="ENN56_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007310808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDS74317.1"
                     /translation="MKAESAVVVTRSAEETRAFGEKFAQTLRSGSVVLLSGSLGAGKT
                     TLVQGICHGLGVTACANSPTFTLINEYVGTRNGEPLRVYH"
     gene            complement(275..1870)
                     /locus_tag="ENN56_02155"
     CDS             complement(275..1870)
                     /locus_tag="ENN56_02155"
                     /inference="COORDINATES: protein motif:HMM:PF08665.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS74318.1"
                     /translation="MSSRIALNTPCMDKMRRRILWVDDEIDLLKSHIIFLDKRGYDVI
                     PVNNGEDAISLIAEERFDLVLLDEHMPGMDGLTTLDEIKSIQPQLPVVMVTKSEEEEV
                     MNEAIGKRIDDYLVKPVNPSQIILAAKRILDARQIREQRLVRDYVAGFRDLDEQMREA
                     GWSEWVDIFARLAQWDLEVHAMHDTSISEMHHTQWRHCNQQFGRFIEEHYADWVANPK
                     HGPIMSPEVVEQFVVPHLRGNEPVYFLVIDCLRLDQWLALEPLVAEYFNVRREYYYSI
                     LPTATPYSRNALFSGLFPIDIAKRYPDIWSLGDDDDYSLNRYEGEMLARHIERLELPN
                     IRTEYAKMHADTDTRDLLRRIQSSRKTTQLYGIVFNFIDHLAHGRSESELLREITPDE
                     AAFRSLTVSWFGRSALFEILTWIADTGGTVVLTTDHGSILGTRATIARGNRDTSTNLR
                     YKFGLNLFCEQRDAVLVKDPATFGLPRITPSTNYIIAKEDFYFVYPTRYHEYDRQYRN
                     SFQHGGISLEEMILPVVTLTPRQ"
BASE COUNT          389 a          547 c          536 g          438 t
ORIGIN      
        1 cagtgataga cgcggagtgg ctcgccgttt cgcgtgccga cgtactcgtt gatgagcgtg
       61 aatgtcggac tgttcgcaca cgcggtgacc ccgagtccgt ggcagattcc ctgcacaagc
      121 gtcgtttttc ccgcaccgag acttccactg agcaacacca cactacccga acgtagggtc
      181 tgcgcaaact tttcgccgaa tgcgcgcgtt tcctcggcgg aacgggttac gacgacggct
      241 gattcagctt tcatgggagg tcgtgcagac tccctcactg tcgcggcgtg agagttacca
      301 cgggaagtat catctcttcg agcgatatcc caccgtgttg gaagctattg cgatattggc
      361 ggtcgtattc gtgatatcgc gtcgggtaga caaagtaaaa atcttccttc gcgataatgt
      421 agttcgtgct cggtgtgatt cgcggcaatc cgaaggtcgc cgggtccttc acgagcacgg
      481 catcccgctg ctcacagaag aggttgagtc cgaatttgta acgcagattc gtactcgtat
      541 cgcgatttcc gcgggcgatg gtcgcgcgcg ttccgaggat gcttccgtga tccgtcgtga
      601 ggaccaccgt cccaccggta tcggcgatcc acgtgagaat ctcgaagagc gccgaccgac
      661 caaaccacga aacggtaaga ctccgaaacg ccgcttcatc gggcgttatc tccctgagga
      721 gttccgactc cgaccgacca tgcgcgaggt gatcgatgaa attgaatacg atgccgtaca
      781 actgcgtcgt ttttcggctc gactgaatcc gccgcagaag gtctcgcgta tccgtatccg
      841 cgtgcatctt cgcgtactcg gtgcggatgt tcggcaactc gagccgttcg atgtgccggg
      901 cgagcatctc cccttcataa cggttcagtg agtagtcgtc gtcatcgcca agcgaccaga
      961 tatccgggta ccgctttgcg atgtcgattg gaaacagtcc gctaaagagc gcgtttcgag
     1021 agtacggcgt cgccgtcggg aggatcgagt agtaatattc gcggcggacg ttgaaatact
     1081 ccgcgacgag cggttccaag gcaagccact gatcgagccg gaggcagtcg atgacgagaa
     1141 aatatactgg ctcattgccc cgcaaatgcg gcacgacgaa ttgctccacc acttccggcg
     1201 acataatcgg gccgtgtttc ggatttgcca cccagtccgc gtagtgttcc tcgatgaacc
     1261 gtccgaactg ctgattgcag tgccgccact gcgtatggtg catttcgctg atcgacgtgt
     1321 catgcatcgc gtggacttcg aggtcccact gcgccagtcg tgcgaagata tcgacccact
     1381 ccgaccaacc ggcttcgcgc atttgctcgt cgaggtcgcg gaatcctgcg acgtagtccc
     1441 gcacgagcct ctgttcgcga atctgtcgag cgtcgagaat gcgtttcgcc gcgagaatga
     1501 tctggctcgg attaacgggc ttcacgaggt aatcgtcgat gcgtttaccg atcgcctcgt
     1561 tcatcacctc ctcttcttcg ctcttggtga ccatgacgac cggtaactgc ggttgaatcg
     1621 acttgatctc gtccagcgtc gtgagcccgt ccatacccgg catatgctcg tccaagagca
     1681 cgagatcgaa acgttcctcg gcaatgaggg atatcgcgtc ttcgccgttg ttcaccggaa
     1741 tgacgtcata tccacgtttg tcgagaaaga tgatatgcga cttgagtaga tcgatctcgt
     1801 cgtcgaccca aaggatccgt ctacgcatct tgtccataca tggtgtgttg agcgcaattc
     1861 gcgacgacac gatgccgtgc ggaatctata gtatgcctga agacgtatcc
//
LOCUS       DSCC01000172            1843 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043104, whole genome shotgun sequence.
ACCESSION   DSCC01000172 DSCC01000000
VERSION     DSCC01000172.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043104"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..659)
                     /locus_tag="ENN56_02160"
     CDS             complement(<1..659)
                     /locus_tag="ENN56_02160"
                     /inference="COORDINATES: protein motif:HMM:PF02578.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HDS74319.1"
                     /translation="MKSDGSRRHATRTNFVRSSKLFPFRMMTATRTKTNRLRVELRNI
                     GIPLWQSPLLSAIPNVRHGFTTRLGGVSTGPYESLNLSLNVGDNPDNVLENRRRLAQT
                     LNLEPRSICLQQQVHGADVIDTLERMGDASICGDAFVTDCVDVPVLVGVADCVPILVA
                     ARDGSAVAAIHAGWRGAVAGVIEATIRRIVARYTFATHDLVAAVGPSIGKCCFEVGDK
                     VA"
     gene            complement(553..>1843)
                     /locus_tag="ENN56_02165"
     CDS             complement(553..>1843)
                     /locus_tag="ENN56_02165"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02824.19,HMM:PF13291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDS74320.1"
                     /translation="LHTTVYTKDNRIVEVQIRTRRMHQVAEEGIARHWLYKEGRVTDD
                     IDSQTKWVRQFLEWAEEIQDPQEFVEQLKIDLFPDEIFVQTPKGEVISLPKGGTALDF
                     AFAVHSEVGFHATTAIVNGKVRTLGYPLSSSDTVKIVTAPNQKPSRDWLGMVKTSKAR
                     TKIRRAIREQEYEFNVDIGRDMLDRRLRKARIHLDDEKWQTVADAFKVSDRDHLYAAI
                     GGGDISVERVLDHLRPNVPDVAPAKTETAPEDFTGGVTLVHLDDPMVRFAQCCNPVPG
                     DPVFGFITRGRGVSVHHRDCPNAAVLHQEPDRIITVEWEEPEVFSGKGPFKTAIIVKS
                     SDRVGILGVISTEISATGANIRHADIRTNDEAGNMAFLIEVKDLRQLKRVISKVRRLP
                     DVYEVRRIASTRDEDELRAIVEALPLPNDDGDEDEDE"
BASE COUNT          400 a          531 c          487 g          425 t
ORIGIN      
        1 gcgaccttat cgccgacttc aaagcaacat ttcccaatcg agggtccaac cgcggccacg
       61 agatcatgtg tggcgaaggt gtatcgcgcc acaatgcgcc gaatcgtcgc ttcgataaca
      121 ccggcgaccg ctcctcgcca tcccgcgtgg atcgccgcaa ccgcagaccc atctcgtgcc
      181 gcgacgagga tcggaacgca atcggccaca ccgaccagca cgggaacatc gacgcagtct
      241 gttacaaacg catcgccaca tatcgacgcg tcacccatac gctcgagagt atcaatgacg
      301 tcggcgccat gtacctgctg ctggagacat atcgaccgcg gttcgagatt aagagtctga
      361 gcgagtcgac gtcgattctc aagcacgttg tccgggttgt cgccaacgtt caaagatagg
      421 ttcagactct catacggacc ggtacttaca ccacccaaac gtgttgtgaa tccgtgccga
      481 acgttgggaa tggcagacaa caatggggat tgccagagtg gtatacctat gttgcgaagt
      541 tccacacgta acctattcgt cttcgtcctc gtcgccgtca tcattcggaa ggggaagagc
      601 ttcgacgatc gcacgaagtt cgtcctcgtc gcgtgtcgac gcgatccgtc ggacttcata
      661 aacgtcgggg agtctgcgca ctttcgaaat gacacgtttg agttgcctta agtccttcac
      721 ttcgataaga aacgccatgt tcccggcctc gtcattcgta cgaatgtccg cgtgacggat
      781 attcgcaccg gtggctgaaa tttccgtaga gatcactccg agtatgccga cgcgatcgct
      841 tgatttgaca ataatcgccg tcttaaacgg ccctttcccg ctgaaaacct caggttcttc
      901 ccattctacg gtaatgattc gatcaggctc ctgatgaagc acagcggcgt tcggacagtc
      961 tcgatggtgt accgatacac cgcgaccacg cgtaatgaat ccgaatacgg gatcgccggg
     1021 caccggatta cagcattgcg cgaaccgaac catcgggtca tcgagatgaa caagcgtaac
     1081 accaccggtg aagtcttccg gagctgtttc ggttttcgca ggtgcaacgt cggggacgtt
     1141 cggacggaga tgatctaata cgcgttcgac ggatatatcg ccaccgccga ttgccgcata
     1201 gaggtgatct ctatcagata ctttaaatgc gtcggcgacc gtctgccatt tttcgtcgtc
     1261 gaggtgaatg cgcgccttgc ggaggcggcg gtcgagcata tcgcgcccaa tatcaacgtt
     1321 gaactcgtat tcctgttcac gtatcgcgcg gcgaatcttc gttcgcgcct tcgaagtctt
     1381 caccattccg agccagtcgc gactgggctt ctgattcggt gccgtgacga tttttactgt
     1441 atcactggac gacagcggat acccgagcgt cctgactttc ccgtttacaa tcgccgtcgt
     1501 ggcgtggaag ccaacttccg agtggacagc gaacgcgaaa tcgagagccg tgccgccttt
     1561 gggtaagctg atcacctcgc ctttcggcgt ctgcacaaaa atctcgtcgg ggaagaggtc
     1621 gatcttcagc tgctcgacga actcctgagg atcctggatt tcctccgccc attcaaggaa
     1681 ctggcgcacc cacttcgttt gggagtcgat atcgtcggtg acgcggcctt ccttgtaaag
     1741 ccagtggcgc gcaatacctt cttctgcgac ttgatgcatg cgacgcgttc gaatctgtac
     1801 ttcgacgatt cgattgtctt ttgtgtaaac cgtcgtatga agc
//
LOCUS       DSCC01000173            2095 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036180, whole genome shotgun sequence.
ACCESSION   DSCC01000173 DSCC01000000
VERSION     DSCC01000173.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036180"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(12..1589)
                     /locus_tag="ENN56_02170"
     CDS             complement(12..1589)
                     /locus_tag="ENN56_02170"
                     /inference="COORDINATES: protein motif:HMM:PF01074.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74321.1"
                     /translation="MPHFPQEREIIRLQVRNRLAEIEASIYRDRQPITGWETVITGEC
                     RDVEQMPQGGWTPFEIVTKSGERTGALKRPIASHYRQRVLSTQWGWFDRSQWFRAMVT
                     VPESMKGWPVVALLEPGAEALCYVNGVPTQGLDPNRSEVILTSCAEGGETYEIAIEAS
                     ATPNAFDDDPIRLFKTPFLAVKDTLAWDFFWDIKVGQELIEALPEGTEKHRLADLVER
                     SVKMVNLNSVSTIDRYHAELKTARDAYLDGRKQFEKSIDSGRVINVGHSHIDTAWLWA
                     IRETRKKSSRTFSTVLKYMDQYPHYTYTQGQPQLYAWIEEHYPEIFEGIKKRIADGRW
                     EANGASWVEQDSNVAGAEAIVRQYLYGRRYFQNVLGSEADVAWLPDAFGFPVTMPQIL
                     AKAGVRAFGTTKINWSQYNRFPYHTFRWRGLDGTEIFAFMPPGHYNGIVNASNARMIW
                     NAYLQKDISGEILNTYGHGDGGGGPTQEMLENLSRLEEFSILPKMEVGNIGPHVDEAV
                     SGVDWEQFPVYNEELLP"
     gene            complement(1625..>2095)
                     /locus_tag="ENN56_02175"
     CDS             complement(1625..>2095)
                     /locus_tag="ENN56_02175"
                     /inference="COORDINATES: protein motif:HMM:PF07748.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDS74322.1"
                     /translation="VLGNRMRISLLRSTVHPDPHADEGRHEFTYSLYPHAGDWRDAGT
                     VRRAYELNAPLIAMNTNPHEGDLPKAFGFISVDRANVLVDCVKKAEDSDATIIRLYEA
                     HGARSSVNLAFAQKPTAVTECDLMEEHDVQVELNGSTIRFSIKPWEIRTFKVQF"
BASE COUNT          420 a          610 c          550 g          515 t
ORIGIN      
        1 ccccggtgca gttagggcaa gagctcttcg ttgtaaacgg ggaattgttc ccaatcgacg
       61 ccggataccg cttcatcgac gtgcggaccg atatttccca cttccatttt cggaaggatg
      121 gaaaactcct cgagtcgcga caggttctcg agcatctcct gggtcggacc gccgccgccg
      181 tcgccgtgtc cataggtatt gagtatttcg ccggaaatgt ccttttggag atacgcattc
      241 cagatcattc gcgcgttcga cgcgttgacg atgccgttat agtgtcccgg aggcatgaaa
      301 gcgaagattt ccgttccgtc cagaccgcgc caacggaacg tgtggtaggg gaaccggttg
      361 tattggctcc agttgatctt cgtagtgcca aacgcgcgca cacctgcttt cgcgagaatc
      421 tgcggcatcg tgaccggaaa accgaacgcg tcgggaagcc atgctacgtc cgcctctgaa
      481 ccgagaacat tctggaagta ccgacgacca tagagatact gccgtacgat tgcttcagcg
      541 ccggctacgt tcgaatcctg ttctacccaa ctcgcaccgt ttgcttccca tcgaccatcg
      601 gcgatccgct ttttgattcc ttcgaaaatc tccgggtaat gttcttcaat ccatgcgtag
      661 agctgcggct gtccctgcgt atacgtgtag tgaggatact ggtccatgta cttgagcacg
      721 gtcgaaaacg tgcgtgaact cttcttccgt gtttcgcgaa tagcccagag ccatgcagta
      781 tcgatgtgcg aatgcccgac gttgatgacg cgacccgaat cgattgactt ctcgaactgc
      841 tttcgcccgt cgagatacgc atcccgggct gtcttaagtt cggcgtggta ccggtcgatc
      901 gtgctgacgc tgttcaggtt caccattttc acagaacgtt cgacgagatc cgcgagtcga
      961 tgtttctctg ttccttccgg tagcgcctcg attagctcct gacctacctt gatgtcccag
     1021 aagaaatccc acgcaagggt atctttcact gcgagaaacg gtgttttgaa aagccgaatg
     1081 gggtcgtcat cgaaagcgtt cggcgtcgca ctcgcctcga tagcgatttc gtaggtctct
     1141 ccaccttcgg cacacgatgt gagaatcact tcggaacgat tcggatcgag gccctgcgtc
     1201 ggtacaccgt ttacgtaaca cagcgcctcg gcaccgggct cgagcaacgc aactaccggc
     1261 caacctttca tactttccgg taccgtaacc attgcgcgga accactggct ccggtcgaac
     1321 catccccatt gtgtgctgag tacacgctga cggtagtgcg atgcgattgg tcgcttcagc
     1381 gcacccgttc tctcgccgga ctttgtgaca atttcaaacg gcgtccatcc gccttgtggc
     1441 atctgttcga catcacggca ttcgcctgtg atcacggtct cccagccggt tatcggttgc
     1501 cggtcgcggt agatcgacgc ttcgatctcc gcaagtcgat tccgaacttg gagccggatg
     1561 atctcccgtt cttgtgggaa gtgtggcatc gtgtatcctc gatttcaagt gtcgctatgt
     1621 gcgttcagaa ctgcacctta aacgtgcgaa tctcccacgg tttgatcgaa aacctgatcg
     1681 ttgaaccgtt gagttctacc tgcacgtcat gttcttccat aaggtcacat tcagtgactg
     1741 ccgtcggttt ttgcgcgaac gcaaggttca cggaactccg cgccccgtga gcttcataca
     1801 gccggatgat tgtcgcgtct gagtcctcgg cctttttcac gcaatccaca aggacgttcg
     1861 ctcgatcgac gcttatgaat ccgaacgctt tcggcaagtc accttcgtgc ggattcgtat
     1921 tcatcgcaat cagcggagcg ttcaattcat acgcgcggcg aacggtgccg gcatcacgcc
     1981 agtcgccggc gtgaggatac agactgtacg tgaactcgtg gcgaccttca tccgcgtgcg
     2041 ggtcgggatg caccgtcgat cggagcaaac taatacgcat ccgattcccc agtac
//
LOCUS       DSCC01000174            2234 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033127, whole genome shotgun sequence.
ACCESSION   DSCC01000174 DSCC01000000
VERSION     DSCC01000174.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033127"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..288)
                     /locus_tag="ENN56_02180"
     CDS             complement(<1..288)
                     /locus_tag="ENN56_02180"
                     /inference="COORDINATES: protein motif:HMM:PRK00565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDS74323.1"
                     /translation="MALSARAVSKYQWSSARKARRVADLVRGKNVNEALNILHFSPAT
                     ASVPIEKTVRSAMANLLDKEEAERLVEDDVIVADVQVNEGPTFKRFQPRAQG"
     gene            complement(380..673)
                     /locus_tag="ENN56_02185"
     CDS             complement(380..673)
                     /locus_tag="ENN56_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDS74324.1"
                     /translation="MARSIKKGPYIEPSLLKKVDEMNSGGAKKVIKTWSRRSTISPDF
                     IGHTIGVHNGNKFIPIYISENMVGHKLGEFAPTRTFRGHPGGGGGGEKTARVR"
     gene            complement(689..1531)
                     /locus_tag="ENN56_02190"
     CDS             complement(689..1531)
                     /locus_tag="ENN56_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDS74325.1"
                     /translation="MPFKSFRPHTPSLRYKMVDAFEDVTKSEPERSLTTNLPKKSGRN
                     NQGRITMRRRGGGHKRRYRIIDFKRNKFGIPARVASIEYDPNRSARIALLFYADGEKR
                     YIIAPDGLVVGDELMSGPEAPIRLGNALPLANIPSGTVVHNVELRPGKGGEIARSAGT
                     RIQLVAREAGMARLRLPSGEVRLVPDVCMATVGEVGHADHDDRVIGKAGRNRWLGKRP
                     KVRGVVMNPVDHPHGGGEGKAPQGGGKNHHPRTPWGKIAKGKKTRKPKASDRLIVSRG
                     RRRK"
     gene            complement(1535..1834)
                     /locus_tag="ENN56_02195"
     CDS             complement(1535..1834)
                     /locus_tag="ENN56_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDS74326.1"
                     /translation="MKTPYEIIKRPVITEKANLLQERANQYAFEVDRAANKLQIRDAI
                     ETLFGVNVISVRVLNVRGKKRRMGIKVRRTGFTSRWKKAVVTLAPDQHIDLVEGA"
     gene            complement(1831..>2234)
                     /gene="rplD"
                     /locus_tag="ENN56_02200"
     CDS             complement(1831..>2234)
                     /gene="rplD"
                     /locus_tag="ENN56_02200"
                     /inference="COORDINATES: protein motif:HMM:PF00573.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDS74327.1"
                     /translation="DMKTNLLRGGGVYGGPKPRSYRKHLPKKMRRAALVSALSIRASE
                     QAVIVIADPAIESGKTRDMAALLKALEIDGKRVLMVNNKPSESMLRAARNIPALVTVS
                     VENLHPYEIMWAEHLLFTDSALSALTGGSES"
BASE COUNT          414 a          714 c          592 g          514 t
ORIGIN      
        1 accttgcgcc cgcggttgga accgtttgaa cgtcggtccc tcatttacct gaacgtccgc
       61 gacgatcacg tcgtcttcca caagccgttc ggcctcctct ttgtcgagga ggttcgccat
      121 tgccgaacgc accgtctttt cgatcggaac gctggcagta gccgggctga aatgcagtat
      181 gttgagcgcc tcgttcacat tcttaccacg aacgagatcc gcaacccggc gtgccttacg
      241 tgccgagctc cattggtact tgctcactgc gcgagctgaa agtgccattg tccaagtttc
      301 ctttttcgcg aacctgcgcg cgtatccgtt ccgctgatgc ggtctctacg gctcttcgcc
      361 gcagcatccc cgccgttact cagcgaacgc gagccgtttt ctcgccgcca ccgccgccac
      421 ccgggtgacc ccggaatgtg cgcgtcggtg caaattcgcc gagcttgtgt ccaaccatgt
      481 tttcgctgat gtagatcgga atgaacttgt taccgttatg cacgccgatc gtatgcccga
      541 tgaaatccgg gctgatcgtc gagcgccgcg accaggtctt gatgaccttc ttcgcgccgc
      601 cggaattcat ttcgtcgacc ttcttcagca agctcggctc gatgtacggg ccctttttga
      661 tggaacgtgc cattcgtacg tacctcggtt atttccgacg ccgaccacgg ctgacgatga
      721 gccggtcgga cgctttcggc ttacgggtct ttttgccctt tgctatcttg ccccacgggg
      781 tccgcggatg atgattcttt ccaccaccct gcggtgcctt cccctcgcca ccaccgtgcg
      841 ggtgatcgac ggggttcatg acaacaccac ggaccttcgg acgcttaccg agccaacgat
      901 tacgtccggc tttgccgata acgcggtcgt catggtccgc atgtccgact tcaccaaccg
      961 ttgccatgca gacatccggc acaaggcgaa cttcgccgga gggcagacgc agacgcgcca
     1021 taccggcttc tcgagcgaca agttggattc tggttcccgc actccgggcg atttcgccgc
     1081 ccttacccgg tcgcaattct acattatgga ccacggtgcc gctcgggata ttcgcgagcg
     1141 gaagcgcgtt accaagacga atcggtgctt ctggacccga catcaactcg tcaccgacca
     1201 caaggccgtc cggtgcgatg atataccgct tctcgccatc agcgtagaac aacagggcga
     1261 ttcgtgccga acggttcgga tcgtattcga tcgacgccac acgcgccgga attccaaact
     1321 tgttccgctt gaagtcgata atgcggtacc gacgcttatg accgccgcca cgacgccgca
     1381 tcgttatgcg accttgattg ttgcgccccg acttcttcgg cagattcgtc gtcagcgaac
     1441 gttccggctc actcttggtc acatcctcga aggcgtcgac catcttgtac cggaggctcg
     1501 gcgtgtgcgg gcgaaatgac ttgaatggca tcggtcacgc tccttcaaca agatcgatgt
     1561 gctgatccgg cgccagcgtt accactgctt tcttccaccg cgacgtaaac ccggtccgcc
     1621 gcaccttgat gcccatacga cgtttcttgc cgcggacatt cagtactcga accgatatca
     1681 cgttcacgcc gaaaagcgtc tcgatcgcat ctctgatctg aagcttattt gcagcgcgat
     1741 cgacttcgaa ggcgtactgg ttcgcccgct cctgcaggag gttcgccttc tcggtaatga
     1801 ccggccgctt gatgatctcg tacggtgtct tcatgactca gagcctcccg tgagtgcgct
     1861 gagtgccgaa tcggtgaata gcaagtgctc agcccacatg atctcgtacg gatgcagatt
     1921 ttccaccgac accgtaacga gcgcgggaat attccgtgcg gcgcgcagca tcgattcgga
     1981 cggcttgtta tttaccataa gtacgcgctt gccatcgatc tcgagcgcct tcaaaagcgc
     2041 cgccatgtcg cgtgtcttgc ccgattcgat cgccggatcg gcaatgacga tgaccgcctg
     2101 ctcactcgcc cgaatcgaca gagcgctcac aagtgcggcg cgacgcattt tcttcggaag
     2161 gtgcttgcgg taagaacggg gctttggacc gccgtagaca ccaccaccgc gaagaaggtt
     2221 cgtcttcata tcac
//
LOCUS       DSCC01000175            1577 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053153, whole genome shotgun sequence.
ACCESSION   DSCC01000175 DSCC01000000
VERSION     DSCC01000175.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053153"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..607)
                     /locus_tag="ENN56_02205"
     CDS             complement(<1..607)
                     /locus_tag="ENN56_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74328.1"
                     /translation="MRFWMDVMRRLEPVLNDHDRLFDAWEAGGCDGLVIGPLVFNQPR
                     LGKGAIPISDEGPSIHVYDPDPSVYARFDVQTSKSPTESLPERRRLLERTLTAAKDRG
                     WSVWIFQPHVGAGPGGPEHHLFDDLTHRAIAARSVDTLQHFPMVDGAVFDGPEWGYEI
                     DPNHRSFLFNDLPESVRDGSARMGYDYTELRGARDTLFERLH"
     gene            complement(625..1185)
                     /locus_tag="ENN56_02210"
     CDS             complement(625..1185)
                     /locus_tag="ENN56_02210"
                     /inference="COORDINATES: protein motif:HMM:PF14520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74329.1"
                     /translation="MTRPHNVTIDDECDDCYRPSFDDEQFVDDAGHLRWKRNTELAAH
                     LRRLHDYLLIGGYPESHAKRYGRLALEIDQLGESVALVEKEGRLKRIRGVGPDIANMI
                     TEFLKKGTTSKYEWWKRETPESVLDLLSISGFGVKTVRMLYMVYAIKSLADLRRAVDD
                     GTLDGVRGIGPRTLANVRRYLDEQTV"
BASE COUNT          304 a          492 c          446 g          335 t
ORIGIN      
        1 cgtgcagtcg ttcgaacagc gtgtcgcgcg caccgcgaag ctcggtgtag tcatatccca
       61 tgcgcgcgct tccgtcgcga acggattcag gcaggtcgtt gaagagaaac gaccgatgat
      121 tcgggtcgat ctcgtaaccc cattccggac cgtcgaagac ggcgccgtcc accattggga
      181 agtgctgtag cgtatccacg gaccgcgctg cgatcgcccg gtgcgtaaga tcatcgaaga
      241 ggtggtgttc cggaccgccc ggacctgcac cgacgtgcgg ctggaatatc cataccgacc
      301 agccacggtc tttcgcagcg gtgagtgttc gttcaaggag acgacgacgt tcgggaagcg
      361 attccgtagg tgatttcgac gtttgcacat cgaatcgcgc gtagacgctc ggatcaggat
      421 cgtagacgtg aatcgacggt ccttcgtcgg aaatcgggat agcgcctttc ccgagccgcg
      481 gttgattgaa cacgagcggt ccgatgacga gcccgtcgca accgccggct tcccacgcgt
      541 cgaacagccg gtcgtggtcg ttcagaaccg gttcgaggcg tcgcattacg tccatccaga
      601 accgcatcgt cggctccttc agtatcagac ggtctgttcg tcgaggtatc tgcgtacgtt
      661 cgcaagcgtt ctcggaccga tcccacgcac gccgtcgagg gtgccgtcgt ctaccgcgcg
      721 acggaggtcg gcgaggctct tgatcgcgta gaccatgtac agcatccgca cagtcttcac
      781 gccgaagcct gagatactga gcagatccag caccgattcc ggcgtctcac gcttccacca
      841 ctcgtatttc gacgtcgttc cttttttgag gaactcggtg atcatgtttg caatatcggg
      901 tccgactcca cggattcgtt tgagtcgacc ttccttttcg acgagcgcga ccgattctcc
      961 gagctgatcg atctccaacg cgagtctacc ataccgtttc gcgtgcgatt cgggataccc
     1021 gccgatcagc aggtagtcgt gcaaacgcct cagatgcgcc gcaagctcgg tgttccgctt
     1081 ccaacgaagg tgccccgcgt cgtcgacgaa ttgctcgtcg tcgaaggacg gtcggtagca
     1141 atcatcgcac tcgtcgtcaa tcgtcacgtt atgcggccgt gtcaccgtcc ggtgaaaacg
     1201 aaagtccgct caacggcgct cccgtgacgt cggtgaagcg catgaacacg ccgtgcatgc
     1261 ccgcgcgatt gtgtactttc acgtcaatcg ttgactcgcc cttggggagc accacttcga
     1321 aaacatctgc atcgaacgtg atgccgcgac cgccgtcgaa acggtgaatc gtcttaccat
     1381 tgagccggag gatcgcgtca tcgtcactac agtagcgcat ttgcacacgt tgttcttcgg
     1441 gactcgtgac cctgcacatc gcataactca ccgagtcgat ggtttccgtg tgtccgccgt
     1501 gcagctcgct gattccgagc acgccgccaa acgcacgccg cacgatccag cgaatccgac
     1561 catgcttccc gtcgtac
//
LOCUS       DSCC01000176            3398 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018895, whole genome shotgun sequence.
ACCESSION   DSCC01000176 DSCC01000000
VERSION     DSCC01000176.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018895"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(220..1395)
                     /locus_tag="ENN56_02215"
     CDS             complement(220..1395)
                     /locus_tag="ENN56_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74330.1"
                     /translation="MANDRNTSALERRARSWFEFDVETPSDADSDAILTRTAALALAP
                     SYAPGDTPPFAPSEWHTLRSDALRFVSALDTSRAERIADIAADLLTSAPLAPPLRFVD
                     TLPEPHPPVSLIFSAWPHATHELWPIMVDALVVNGAQAVHDTWLDDDNRVRKAIRYDV
                     EADRLIEGENATPQHPKRSLLLLGPVDNERVWHQQLDESLRASGIEVLNPYRMALIAD
                     DKWATYERWRAASVATPETRLITRATIDTAEESLRHIRQLGECVVVKPRCGTAARNVT
                     ITTDIDEALDAVRSILRDDDAIIQPFQNDLRWSGESDTAYPTVLRINVVRGECGTVRA
                     ESGYLHVAPTAEQLVASVAQFGRGRSICRNSLEIGGMPITESETLVFESIAEKPFAR"
     gene            complement(1385..2053)
                     /locus_tag="ENN56_02220"
     CDS             complement(1385..2053)
                     /locus_tag="ENN56_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74331.1"
                     /translation="MSTDKPTARDRRKHWHSSHPITLNSTQLITNVGRGGTLELLRPT
                     DIRPEHREAILHALDEAGRDVMEAMAAFESRAAKQYEKETGDPVGADLTGVSYGIPRY
                     LMLDFLLRPEFDRPGDLVEIMPDVDETGHRIGSQFLLSDGTESFVGKITGWTMILIEP
                     NIGIGLWDRVALREIEHERERAREAGKPMDWNLVGRNARVVLRDLTRAGADYLAALAK
                     THGK"
BASE COUNT          631 a          951 c         1060 g          756 t
ORIGIN      
        1 gggtatgaat acgaatttct ctctccggtt acacttcgga ccagagtgta aatgtcaccc
       61 ccggatcgcc cggtagctcc caatcgggtg tgagatagcg gctgtgtggg agacccgccg
      121 gtcgtggatt cgcatcgatc acatatggcc gaatcacacc ggttgcgtcg cgctcggcaa
      181 cgatatcgac accaacaaga caggtcgtcg catcatccgt cagcgcgcga acggcttttc
      241 cgcgatggat tcgaagacca aggtttcgct ttcggtgatc ggcattccac cgatttcgag
      301 tgaattgcga cagatcgaac ggccgcgccc gaattgcgcg acactggcta cgagctgttc
      361 ggcggtaggt gcgacgtgga ggtagcccga ttccgcgcga acggttccac attcgccacg
      421 tacgacattg attcgcagaa ccgtcggata tgccgtatca gattcgccgc tccatcggag
      481 gtcgttttgg aacggctgta taatcgcgtc gtcatcacgc agtattgacc ggaccgcatc
      541 gagagcttcg tcgatgtcag tggttatcgt gacattgcga gcggcggttc cgcacctcgg
      601 tttgacgaca acgcattcgc cgagttggcg gatgtgccgt agcgattcct cggccgtatc
      661 gatcgtcgct cgggtaatca accgcgtctc gggtgtcgca acgctcgctg cgcgccaccg
      721 ctcgtatgtc gcccacttgt catcagcgat cagagccatg cgatacggat tgagtacctc
      781 gattccggaa gcgcgcaacg attcatcgag ttgctggtgc catacgcgct cgttgtcaac
      841 tggtccgaga agcagaagag accgtttcgg atgctgcggt gtcgcgttct cgccctcgat
      901 gagtcggtcg gcctcgacgt cataccgaat cgctttgcga acgcggttgt cgtcgtcgag
      961 ccacgtgtcg tggaccgcct gcgcaccatt gacaacgagc gcgtcgacca taatcggcca
     1021 gagttcatgt gttgcgtgcg gccatgcgga aaagatgagc gacacgggtg gatgcggctc
     1081 gggaagggtg tcgacgaagc gcagtggtgg tgcgagcggt gcacttgtga ggagatcggc
     1141 ggcgatgtcg gcgattcgct cggcacggga agtgtcgaga gcgctgacga atcggagcgc
     1201 atcactccga agcgtgtgcc attctgatgg ggcgaatggc ggcgtgtcgc ccggcgcgta
     1261 tgatggggcg agggcaagcg ccgccgtgcg tgtgagaatc gcgtcggaat cggcgtcgga
     1321 tggtgtttcc acgtcgaact cgaaccatga gcgcgcccga cgttcgagcg cgctcgtgtt
     1381 acggtcattt gccatgcgtt ttcgcgagtg cggcaaggta gtctgcgccg gcacgggtga
     1441 ggtcgcgcag tacgactcgt gcgtttcggc cgacgagatt ccaatccatc ggttttcccg
     1501 cttcgcgcgc acgttcgcgc tcgtgctcga tctcgcgtag tgcaactcga tcccacaagc
     1561 cgatgccgat gttcggttcg atgaggatca tcgtccagcc ggtgattttc ccgacgaacg
     1621 attcagtccc gtcactgagg aggaactgcg aaccgatacg atgcccagtc tcatcaacat
     1681 cgggcatgat ctcgacgagg tctccggggc ggtcaaattc cggtcggagg agaaaatcga
     1741 gcatgaggta ccgcggaatt ccgtacgaga cgcccgtgag gtccgcgccg acggggtcgc
     1801 cggtttcctt ttcgtactgc ttcgcggcac gggattcgaa tgcggccatc gcctccatca
     1861 cgtcgcgacc cgcttcgtcc agcgcgtgga gaatcgcttc gcggtgttcc ggacgaatat
     1921 ccgtcggacg gagcagctca agcgttccgc cacgaccgac gtttgtgatg agttgcgtgc
     1981 tgttgagcgt gatcgggtgg ctgctgtgcc aatgtttgcg acgatcgcgt gcagttggct
     2041 tatccgtgga catgatgacg cggaacgatc cgaagagcgg tgtctgcggt tgccgatctc
     2101 gcgcaatcgg acgccgccat tcactgatct gtcggtcggc gaatcggctg aggaagtctt
     2161 ccgtcgccca gagttccggt gatgagatga tgacttcctg tacggcgacg ttgtgtttca
     2221 gcgatatctg atagaggaac tcggtagctt cgttgagtgc cgactcgatg atctctccgc
     2281 ctgcttagcg taattcgaac gccttcgcac cacgtccgcc cgattcagcc gcatctttga
     2341 tcatgatgat acgatgcttc cgcgcgaact tggtaagacc gatgaagtgg gttttcttcc
     2401 gaccgctctg aatttcgttg agtgttcgcg tttcgtcatc gttcttttcc gtccattcgg
     2461 cgagccaatc gatctcgtcg gggatatgga cgtgtgactt ttcaaatatc tccggatact
     2521 tcgccttgtc gtacgtagtc cacgtgtgga aacgggattg gacactgaag ggaagcccga
     2581 gtcgctcagt ctcgccgcgt gtgtagatcg gattcccatg catagggagg atcaccgcgg
     2641 tggttatcgg acgtgagaac cgcgcggcaa tgcgaacgcc ctttggtccg gcatcgcgtt
     2701 cggcgagggc cttcgcgccg gtatcgttca atccccagtg ctgcttcaac gcagcgacgt
     2761 aggtgttcgt gtcgacatag aagtggccgt catcgatgac gacacggttc ttttcgacgc
     2821 gcgggcggcg tgtgaggatg atgttcgccg ctggttgccc atcggtgacg agctcgcgcg
     2881 ccaaggtcgg tccttcggcg agtagtcgcg acacgtgtgt tcggccgacg ggatacatcg
     2941 cgatgatgtt gacccgtccg tcggcatcga taagcgcagg atcgaactcg ccgaaaacag
     3001 gcggaagtga aaacccgtcg tttcggatga acgcgatttc tcgtagctga ttgtatgcgc
     3061 gccaccgctc ttcgaggtcg ctggaaccgt cggcaccaat cgcgacggcg acttcgtttt
     3121 cgccgatgcg cccaataagg aggtcccgct gtttcgcgga aaggtaccgt acctctgtgc
     3181 ggctcgtgat gaggtttcgc aagtactgat agagccaacg ataatgccga tcggcgagct
     3241 cgtagagttc gtcgtcatgg aactcggcga atccaacacc gcgtgaatac gcccacggtc
     3301 ggtactcgta gaagtgatcg ttcagcgtct cggcgaagaa tgtcgcaatc gcgcgccaca
     3361 attcagcgtc gtcgacaccg tttggatcac gacggcga
//
LOCUS       DSCC01000177            3015 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022218, whole genome shotgun sequence.
ACCESSION   DSCC01000177 DSCC01000000
VERSION     DSCC01000177.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022218"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..962)
                     /locus_tag="ENN56_02225"
     CDS             complement(<1..962)
                     /locus_tag="ENN56_02225"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS74332.1"
                     /translation="MDDLKTLAHIACEEAIRAGADFADVSISRGRSTRLNVEKNGIHD
                     MRERRSAGVSVRAIVNGGRAFASTSRIDEAFIRETAQRAAAAAKIAEPDPDFVSLPGP
                     AEYAAIEGLRDDRVAAIDPPTLIAALMTEVDGARAVDSRAVVEGGASASESEHAIVNS
                     LGVEGVSASTRVSLHVSCVIRVGDDVGSYYDYTGGRVLDDFSPGGLGARVCEQTKRFL
                     NQRTCKTAVLPIIAGPLAASSFIGALAGAANAEHIQRGRSYFIGKRGEKIGPDFLTIV
                     DDPFIPRGLGSGPFDDEGAPAQRVAIIENGVLANWLHGSYTANKA"
     gene            complement(973..2367)
                     /locus_tag="ENN56_02230"
     CDS             complement(973..2367)
                     /locus_tag="ENN56_02230"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS74333.1"
                     /translation="MKELLEQARDRAVTRGVQFMDARYSASDQTGVRVQDNRADELNQ
                     MSGSAVGIRVLVDDAWGFASSDVLTADALSASLDAAIAMARGSAERVLDPGVVALVQP
                     VVDTVRVAVERDPRDIELAEKVRLATAYETAGRAAGGDAISNSFVGIVDSWSREIICN
                     TVGTLIEQEKLRTLLFAMYTASDGTTRQRSFEARAAQRGFELVDETSAEDLSVKAART
                     AVSLLTAKRCPSGRFTVVLDPSGAGVFAHEVLGHNAEADYVFAGTSIIEGKVGERIGS
                     EHVTIVNDATIPRANGSYIYDSEGVPGARIPIIERGVLTGYLHSLESAARLGAVPNGC
                     GRAQDGHSLPQVRMSNTFFAGGEKSFDELIADIDTGMLIEDAGGGYVYPERGTYTVTA
                     GKGRMIRNGELAEPIREITFTGVLLETLQNVEGATKDIELLPNTCGKGGQVVHVTTGG
                     PYVRIRDVVVGGQE"
BASE COUNT          579 a          960 c          871 g          605 t
ORIGIN      
        1 gctttgttcg ccgtatagct gccgtgcagc cagttcgcca gcacgccgtt ctcgatgatc
       61 gcgacgcgtt gcgccggcgc tccttcgtcg tcgaacggac cggaaccgag tccgcgcgga
      121 atgaacggat cgtcgacgat cgtcaggaaa tcgggaccga tcttctcgcc acgtttcccg
      181 atgaaatacg accgcccgcg ttggatatgt tcggcgttcg ccgcaccggc aagcgcaccg
      241 atgaacgaac tcgcggcgag cggtccggcg atgatcggga gaacagcggt tttacacgtg
      301 cgctgattca gaaagcgctt cgtctgctcg caaacgcgtg cgccgagtcc acccggcgaa
      361 aagtcatcga gtactcgtcc gcccgtgtag tcgtagtatg agccgacgtc gtcgccgaca
      421 cgaatgacac aactgacatg caatgatacg cgtgtcgacg cgctcacacc ttcgacgccg
      481 agcgaattca cgatcgcgtg ttccgattcc gatgcgctcg caccgccttc aacgacggcg
      541 cgcgaatcaa ccgcgcgagc accgtccact tcggtcataa gcgcggcgat cagtgtcggc
      601 ggatcgattg cggcgactcg gtcatcgcgt agaccttcaa ttgcggcgta ttcggcgggt
      661 ccgggcaacg agacgaagtc gggatccggt tcggcgatct tcgcagccgc cgcagcgcgc
      721 tgagccgttt cgcgaatgaa cgcttcgtcg atgcgcgatg tcgaagcgaa cgcgcgtcca
      781 ccattgacga tcgcgcgtac cgatacgccg gcgctccggc gttcgcgcat gtcgtgtatg
      841 ccgttcttct cgacattgag ccgcgttgaa cgcccacgcg atatggacac gtcggcgaag
      901 tcggctcccg cgcgaatggc ttcttcgcac gcgatatgtg ctaatgtttt gagatcgtcc
      961 attgtgtccc cctcactcct gaccgccgac cacaacgtcg cgaatccgga cgtaggggcc
     1021 acctgtggtt acgtgaacga cttggccacc cttgccgcac gtgttgggca gaagctcgat
     1081 atctttcgtc gcgccttcga cgttctgcag cgtctccaag agaactcccg tgaaggtgat
     1141 ttcgcgtatc ggttccgcaa gctcgccgtt gcggatcatg cggccctttc cggccgtgac
     1201 cgtatatgtt ccgcgctcgg ggtacacgta cccgccgccg gcgtcctcga tcagcatgcc
     1261 ggtgtcgata tccgcaatga gctcgtcaaa cgacttctcg ccaccggcga agaacgtatt
     1321 actcatacgc acctgcggga ggctgtgccc gtcctgtgcg cgcccgcatc cgttcggtac
     1381 cgcacccagc cgcgccgctg attccagcga gtggaggtat cccgtcagga cgcctcgctc
     1441 gatgatggga attcgtgcgc ccggaacgcc ttccgagtcg tagatgtacg acccattcgc
     1501 ccgcgggatc gtggcgtcgt tcacgatagt gacgtgctcc gagccgatgc gctcgccgac
     1561 tttcccttcg atgatcgacg tgccggcgaa gacgtaatcg gcttcggcat tgtgaccgag
     1621 tacctcgtgg gcgaaaaccc cggcgccact cggatcaagc acgacggtga atcgccccga
     1681 cggacaccgt ttcgccgtaa ggagcgaaac cgccgtacga gccgctttca cactcaggtc
     1741 ttcggcggat gtctcatcga caagctcaaa gccccgttgc gcagcgcgtg cctcgaacga
     1801 tcgctgtcgg gtcgtgccgt ctgacgcggt atacatcgcg aagagtagtg tgcggagttt
     1861 ctcctgctca atcagcgtcc cgacggtatt gcagatgatc tcacgcgacc acgagtcaac
     1921 gattcccacg aacgagttgc tgatcgcgtc accgcccgca gcgcgccccg cagtttcgta
     1981 ggcggttgcg aggcgcacct tctccgccag ttcgatatcg cgcgggtcgc gttccaccgc
     2041 cactcggacc gtatcgacca ccggttgcac caacgcgacg acacccggat cgagtactcg
     2101 ttccgccgat ccacgcgcca tggcgatcgc cgcgtcgagg ctcgcactga gcgcatccgc
     2161 tgtgagaacg tcggaggatg cgaatcccca cgcgtcatcg acgagcacgc ggataccaac
     2221 cgccgagccg ctcatttgat tgagttcgtc cgctcgattg tcctgtacac ggaccccggt
     2281 ctggtcacta gcggagtacc gcgcatccat gaattggacg ccgcgtgtga ctgcgcggtc
     2341 gcgcgcctgt tcgagaagtt ccttcatgaa gtgttctcgt ttcttgactg tggcgggatt
     2401 tttgaaggta tcggatacca cactaactga ggtgtgagta tataaggtta tgggtaaaat
     2461 ccgcaacctg cgagcgggag gtagaaggaa tcattctcac gaggagacgt taaggcgaag
     2521 acatccgtct tcgcccaacg cggctccata cgaattcgca caacaattcg gtgccattca
     2581 caccgaccgg cgaaggcgtg ggttgcgcgg tgcgactgaa gtcgctcgct acaattacaa
     2641 agtctgccta cgcagactcg cgtctcctcg ctgacgctcg gagacgtcag acgaagcgcc
     2701 tcagcgcttc gtcttaacga actcgaacgc acgtgagagt tcgccgcgta ggcggacttc
     2761 cttgcgaagc cgggttaaac aaaaagagcc cgcgaattta ttcgccgggc tatctgccgc
     2821 ttcgggttcg tttcggagaa acgtgcgttt ctcccatcgc ggcttcaaac gattccgcgc
     2881 aacaatccgg tgccatatac gccgcgttcg gagacgttgg gcgaggcatg cctcgcaccc
     2941 tacagcgaag caactcgata cctcgttaaa cacaaaaggc gaagacatcc gtcttcgccc
     3001 accacggttt caaac
//
LOCUS       DSCC01000178            2303 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031717, whole genome shotgun sequence.
ACCESSION   DSCC01000178 DSCC01000000
VERSION     DSCC01000178.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031717"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..652
                     /locus_tag="ENN56_02235"
     CDS             <1..652
                     /locus_tag="ENN56_02235"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02954.17,HMM:PF14532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS74334.1"
                     /translation="FLDEIDGLDISLQGKLLRVLQERQFERVGGETTITTDVRLIAAT
                     NSDLEETVEKGEFRKDLFYRLNVVRIEAPSLAERREDIPILVKHFITKYAQENGKAIK
                     GVDSAAMSFLQGYEWPGNVRELENAIESAVVMCSGDMITSDDLPEHIKNRAPEEVLVR
                     LGTPLSDIEHLVIEATLRMTGGNKTQTANVLGIGVRTIHDKLRRYKEEGREIVLPE"
     gene            757..1083
                     /locus_tag="ENN56_02240"
     CDS             757..1083
                     /locus_tag="ENN56_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74335.1"
                     /translation="MAETVQALRAREYLTTYVRIGYELVLIVSGPTGEVAVIDLSHDA
                     AAVWRDAWAGMRKVLPRMPVVAVVCGDEYTEWEQEHAPVISVSNPESGDAVVQAVEKL
                     LKPETH"
     gene            1142..1795
                     /locus_tag="ENN56_02245"
     CDS             1142..1795
                     /locus_tag="ENN56_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HDS74336.1"
                     /translation="MEHSILWFYGAALLALFPIVDPITAGFSFAGMAEGRDVKWRNRQ
                     ALLASIYMIVILFLFQFLGTALLNFFAITLEAIRIAGGVMIWYMAMEMLGGNERLSAD
                     EKSEGTLKDDIAFTPMAMPLLSGPGAIAVILTLNATSATVVSDIAIILAVITTATVSY
                     ITLRFASEIQAHISDTGRIAFRKILGFLLLCVSVQFIMSGVAPLFNGSASALMQFSH"
     gene            1876..>2303
                     /locus_tag="ENN56_02250"
     CDS             1876..>2303
                     /locus_tag="ENN56_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS74337.1"
                     /translation="MEMKTPQHKRILLEQVLVQSADPFIFMDVSGRIRIWNRGAERVF
                     GWTHEEAVGQEVDIIFPEVADIHGDVEDLRAGHAPYDDNVVVQNYETTLRGRSGARIP
                     VKITVTLITDADGAILGSSITVRDVSREHLLENQLRSRTG"
BASE COUNT          510 a          630 c          675 g          488 t
ORIGIN      
        1 gttcctcgac gaaatcgacg gactcgacat ctcccttcaa gggaagctgc tccgagtctt
       61 gcaggaacgg caattcgagc gcgttggcgg cgagacgacg attacaaccg atgtacgtct
      121 catcgccgcg acgaactccg atctcgaaga gacggtggag aagggcgagt tccgaaagga
      181 tctcttctac cgcctgaacg tcgtgcgaat cgaggcgccg tcgcttgcgg aacgacgcga
      241 agatattccc attctcgtga agcacttcat cacgaaatac gcgcaggaga acggcaaggc
      301 gatcaagggc gtcgattccg cggcgatgtc gttcctacag ggatacgagt ggccgggaaa
      361 tgtccgcgag ttggaaaacg ccatcgaaag cgccgtcgtg atgtgttcgg gagacatgat
      421 cacatcagat gaccttccgg agcatatcaa gaaccgggcg cccgaggaag tgctcgttcg
      481 actgggaacg ccgctttcgg atatcgaaca tctggtgatc gaagcaacac ttcgcatgac
      541 cggcggaaat aaaacgcaga ccgcgaatgt gctgggcatc ggtgttcgca cgattcatga
      601 caagttacgt agatacaaag aagagggacg agaaatcgtt ctgcccgagt aaggagtcgc
      661 cgcaagccga cgacgggttg cggcggatcg ggggttgcgc agatggtgtc atcggttccg
      721 agggatcttc ataacgaggg aaacgagcct cggtggttgg ccgagacggt ccaagcactg
      781 cgcgcgcgcg aatatctgac gacgtacgtt cgcattggat acgaactcgt cctcatcgtc
      841 agcgggccga ccggagaggt tgccgttatc gatctttcgc acgacgcggc agccgtctgg
      901 cgcgatgcat gggcggggat gcggaaggtg ctcccccgta tgccggtggt tgcagttgtt
      961 tgcggagacg aatataccga gtgggagcaa gagcacgcgc ccgtcatctc ggtgtcgaat
     1021 ccggaaagcg gcgacgcagt agtgcaggcg gtcgagaagc tcctaaaacc cgaaacgcat
     1081 tgattccgca ggcgacgttt acacgataca cgtaggtctc ttttggtcag cgagttggtc
     1141 catggaacac tcgattctct ggttctacgg tgcggcgttg cttgcgttgt tcccgattgt
     1201 cgatcccatc actgcgggat tcagtttcgc cggcatggcg gaaggtcgcg acgtcaaatg
     1261 gcgcaatcgt caagcgctgc tcgcatcgat ctacatgatc gtcatcctgt tcctctttca
     1321 attccttgga accgctctcc tcaatttttt tgcgattacc ctcgaagcga tccgaatcgc
     1381 cggtggcgtg atgatctggt acatggccat ggagatgctc ggcgggaacg aacgtctctc
     1441 ggcggacgaa aagagcgagg gaacattgaa ggatgatatc gcgtttactc cgatggcgat
     1501 gccgttactc tccggacccg gtgcgatcgc cgttatcctc acgctcaatg ctacttcggc
     1561 aacagtcgtc agcgacatcg caatcatcct cgcggtgatc accacggcca cggtcagcta
     1621 tatcaccttg cggttcgcct cggaaatcca agcgcatatc agcgataccg gacgaatcgc
     1681 gtttcggaag attctcggtt tcctcttact ctgcgtttcc gtacaattca ttatgtcggg
     1741 cgttgcgcca ttgttcaacg gtagtgcgtc tgctcttatg cagttctccc actaaatcgg
     1801 gaacggtgaa cgtcaccttt cacgattcaa gtacgatttc accgccggaa tccggcgaaa
     1861 gtattgaact cggcaatgga gatgaagaca ccgcaacaca aacgaatact cctcgaacag
     1921 gtcctcgtgc agtcggccga tccgttcatc ttcatggacg tatccgggcg tatccgtata
     1981 tggaatcgcg gtgcggaacg ggtatttggc tggacccatg aagaggcggt cgggcaggaa
     2041 gtcgatatca ttttcccgga agtcgccgat atccacggcg atgtcgagga ccttcgcgcg
     2101 ggacacgcgc cgtacgatga caacgtcgtc gtgcagaact acgaaacgac gctgcgcggc
     2161 cgttcaggtg cgcgtattcc cgtaaagatc accgtgacgc tcattacgga cgccgatggt
     2221 gctattctcg gcagtagcat cactgtgcgc gatgtcagcc gggagcacct cctcgaaaat
     2281 caactccgct cacgcaccgg cga
//
LOCUS       DSCC01000179            2296 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031876, whole genome shotgun sequence.
ACCESSION   DSCC01000179 DSCC01000000
VERSION     DSCC01000179.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031876"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..489
                     /locus_tag="ENN56_02255"
     CDS             <1..489
                     /locus_tag="ENN56_02255"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDS74338.1"
                     /translation="PDTSVEVLIPDFKGDPAALERVTNARPEILNHNVETVPRLYRRV
                     RPQARYEWSLGVLGAAKKHGVTTKTGIMVGLGETMDEIADVMRDLVRMRCDILTIGQY
                     LQPTPRHLPVERFVTPEEFAELKRIGEGLGIGHVESGPLVRSSYHAAEQAERFRPGPK
                     RQ"
     gene            507..1439
                     /locus_tag="ENN56_02260"
     CDS             507..1439
                     /locus_tag="ENN56_02260"
                     /inference="COORDINATES: protein motif:HMM:PF14257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HDS74339.1"
                     /translation="MISNRYRGLTAILSFAALAIVVACGNDDGASAAKSVEQARVETF
                     AARAAMNAPPGRGGQTVADTDMFVAAGQSQPDAGVGRPNVPDPVERKLIRHANATVEV
                     DSVDAAIERLVALASELGGYVSSQNRSLLYDERLRGSVTLRIPGTHVDETLDTVRELG
                     RIRDERVWVDDVTEEYYDLSIRLDNARDERGKLREILERARTVEDLLKVQRELSRVTG
                     EIERMTGRMRRLTNQIEFSTITVEVVEKPRLVADTDTAIGKIVAAFRDMVDVFWSTIA
                     AIIRFVGVAIPIVIVVALVVWILVVVVRKWRRKP"
BASE COUNT          490 a          610 c          704 g          492 t
ORIGIN      
        1 cccgacacgt ccgtggaagt gttgataccg gacttcaagg gtgatccggc cgcactcgaa
       61 cgtgttacga atgcgcgtcc cgagatcctc aatcacaacg ttgagacggt cccgcggttg
      121 tatcgtcggg tgcgtccgca ggcgcggtac gagtggtcgc ttggcgtatt aggtgcggcg
      181 aagaaacacg gtgtgacaac caagaccggc atcatggtgg ggctcggtga gacgatggac
      241 gaaatcgccg atgtaatgcg cgatctggta cgcatgcgat gcgacatctt gacaatcgga
      301 cagtatcttc agcctacgcc gcgccatctg ccggttgagc gatttgttac accggaagag
      361 tttgcggaac tcaagcggat cggtgaaggt ctcggcattg gccacgtcga gagtgggccg
      421 ctcgtgcgga gctcctacca cgcggcggaa caagctgagc gattccgtcc gggtccgaag
      481 cggcagtgat cagaggaggg aaggtcatga tttcgaatcg atatcgtgga ctgactgcga
      541 tactgtcgtt tgccgctttg gccatagtgg tcgcgtgcgg gaacgatgat ggggcgtccg
      601 cggcgaagtc ggtggaacaa gcgcgtgtcg aaacgttcgc tgcgcgggca gcaatgaacg
      661 caccacccgg gcgtggcggc caaaccgtgg cggatacgga catgttcgtc gcggcagggc
      721 aatcacaacc ggacgccggt gtcggtagac cgaatgttcc cgatcccgtc gaacggaagc
      781 tcattcgcca tgcgaacgcg acggtggaag tagattcagt tgatgcggcg atcgagcgcc
      841 tcgtagcgtt ggcgagtgag cttggtggat atgtctcgtc gcagaatcgg tcgcttctgt
      901 acgatgaacg gttgcgcgga agtgtgacat tgcgtatccc cggtacacac gtcgatgaaa
      961 cgctcgacac ggtgcgcgaa ctcgggcgga ttcgtgatga acgcgtgtgg gtcgacgacg
     1021 taacggaaga gtattacgat ctcagcatac ggcttgataa cgcacgcgac gaacgtggaa
     1081 agttgcggga gatacttgaa cgcgcgcgca ccgtagaaga cctgctgaag gtgcaacgtg
     1141 agctgagtcg cgtcacgggc gagatcgagc gtatgaccgg tcggatgcgt cgactcacaa
     1201 atcagatcga attctccacg attacggtcg aggtggtaga aaaaccgcga ctcgttgctg
     1261 atactgatac cgcaatcggc aagatcgtcg ctgcgttccg cgacatggtt gatgttttct
     1321 ggtcgacaat cgccgcgatc atccgttttg tgggtgtggc gataccgatc gtcatcgttg
     1381 ttgcgctcgt cgtgtggatt ctcgtcgttg tggttcgaaa atggcgaaga aagccgtaat
     1441 cgcccaatac cactcgttcg tacaaccgaa acgtcggaat ccgacaccca tcgaatccca
     1501 gtgatattct tttacgcggt gttgttcgcc accggaatga atatcgtgga aggcggttac
     1561 gaggcagttt cacgaggatt aattcggtat gaagaatcca ttgcgatatc gtgtcgagat
     1621 atgacacccg tgcgatctac tcccaaagaa gcagtcgtac ggggccgatc aatcctgcga
     1681 ttttcggttc cgtatcgaat gacgcgacct tttgtgcata actattgtac caaccacgtt
     1741 gtttgaggtc ttcgacgacg gcttcggacg acatgagtcg atgcagcgtg ctcgttaccc
     1801 gcataccgat ggtgttgtca cccggaccga ttgcttgcgt gacatcgatc gtatacggtg
     1861 gccatatgcg gcgacctatc ggaacgctgt tgacgacgac ttgcgccacc acgcccaccg
     1921 ttccgagatc gagcacaacc ttgcgcggcg tggagtcgag ccgcacggtc gttacgtaat
     1981 cgacggtgcc acagacggtc gcaccgaaga gttcatccca gtgcgtgagt tcgatttttt
     2041 caagcggtgt tgactccgac ttgcgttgcg cgagctcgcc gtcctcatac gcccattcag
     2101 ttaccaagcg cgcagcccat ggaccgggca catcgacggt atgcgccggg ctttccacat
     2161 cgaccgaccg atccgtgtca acctcgccct caccgacaat cagggctacc gcagatttcg
     2221 gtgcgattgt gagacgaacg gaatctttga ccatgtcggc atcgacgagc gtgtcgaatt
     2281 ccggatcgta aagccg
//
LOCUS       DSCC01000180            1815 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043991, whole genome shotgun sequence.
ACCESSION   DSCC01000180 DSCC01000000
VERSION     DSCC01000180.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043991"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..291)
                     /locus_tag="ENN56_02265"
     CDS             complement(<1..291)
                     /locus_tag="ENN56_02265"
                     /inference="COORDINATES: protein motif:HMM:PF04613.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74340.1"
                     /translation="MADRHRVTRTSMESVRVADIAAAVRGTWHGDPERLVTGVAALHD
                     AGPDDLSFYTDSRFRTELSHTKAGVIVIAPADASLIHDRALVVDRPRVAFRQI"
     gene            complement(313..834)
                     /locus_tag="ENN56_02270"
     CDS             complement(313..834)
                     /locus_tag="ENN56_02270"
                     /inference="COORDINATES: protein motif:HMM:PF03938.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDS74341.1"
                     /translation="MRRMLIGLSAVMITALLFGFASEASAQMKIGYVFMDEIRMRAQP
                     YQDAQSQLQQAATERQQEAEQLQAEIGRMQEQLERQRGLLTEQRRTERETAIRQKLME
                     YEQWASTAQQDLGRRQMELLQPLDERVLNLIQGVSADRGYDLVIDGTAIAYMRNHEEH
                     NLTDAVIEALNAE"
     gene            complement(1005..>1815)
                     /locus_tag="ENN56_02275"
     CDS             complement(1005..>1815)
                     /locus_tag="ENN56_02275"
                     /inference="COORDINATES: protein motif:HMM:PF01103.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74342.1"
                     /translation="VTEETRGFSLRLGRRLRWPDDYSRVYASYSFRNERYTFPSYFTD
                     ADKYYYVQDPSNPSLISSSIGLGYSRDSRNLPLFPTAGTYLAYDFQHAGGPWGGDVDY
                     RKHTVQANMYLPVVDIRGWIPALAIKTTFGQINTSEQTNVPLSERFRPGGISFDGQIR
                     GYPDYSIGPRTGGGYTSGGFTMFITTAELAFPIVNQQIYAVAFADAGNAWRDVKEMDL
                     NKLNRSSGFGVRFILPLAGIIGLDFAYGYDRQSYMGGRQWETHFQFGQTLF"
BASE COUNT          355 a          525 c          542 g          393 t
ORIGIN      
        1 gatctgccgg aatgcaacac gcggacggtc gacgacgaga gcgcggtcgt ggatcagaga
       61 tgcgtcggcg ggcgcgatga caatcacgcc cgccttcgtg tgcgaaagtt ctgtgcgaaa
      121 ccgtgagtcg gtgtagaagc tcaggtcgtc cgggccggca tcgtggagcg cggcgacacc
      181 ggtcaccaac cgttcgggat cgccgtgcca cgtgccgcga actgcggcag cgatatcggc
      241 gacgcgaacc gattccatcg aggtgcgcgt cacacggtgc cgatcggcca tggagcgagt
      301 ctcccggcga tgttattcgg cgttgagcgc ttcaatcacc gcgtcggtga gattgtgctc
      361 ttcgtggttc cgcatatagg cgatggcggt gccgtcgatc acgagatcgt agccgcgatc
      421 ggcggagacc ccttggatca agttcaggac acgctcgtca agcggctgaa gcagctccat
      481 ctgccgccgt cccagatcct gttgcgccgt cgatgcccac tgttcgtatt ccataagctt
      541 ctgccggatc gctgtttcac gttccgtgcg gcgctgttcg gtcaacagcc cgcgctgacg
      601 ttcgagctgt tcctgcatcc ggccgatctc agcctgcagc tgttcagctt cctgctgacg
      661 ctcggttgca gcttgttgga gttgagactg ggcatcctga tatggctgag ctcgcatacg
      721 gatctcatcc atgaacacat acccgatctt catttgcgcg ctcgcttcgg atgcgaatcc
      781 gaacaggagc gcggtaatca tcacggcgct caagcctatg agcattcgac gcattgtgca
      841 taacctccga gaggggtgtt gtgtgcggtt cgtgcgactt cggtcgatac cgcgcgtata
      901 cgtgataaga aactgtgtcg tgccgggtta cgaatatccg gcttcatcgc gcctgaacgc
      961 aacgcctctt ttttccgatc ggcgggccga tctccggatt cggctcagaa cagcgtctgt
     1021 ccgaactgga agtgcgtttc ccattgtcgg ccgcccatat aactctgccg atcgtatccg
     1081 taggcgaaat caagtccgat gataccggca agcgggagga tgaaccgcac cccaaaaccc
     1141 gacgaacgat tcagcttgtt caggtccatt tccttgacat cacgccacgc gtttccggca
     1201 tcggcgaatg caacggcgta aatctgttga ttgacgatcg ggaacgccaa ttccgccgtg
     1261 gtaatgaaca tcgtaaaccc gccggaggtg tatccaccac cagtacgcgg accgatacta
     1321 tagtcgggat agccgcgaat ctgcccgtcg aagctgattc cgccgggacg gaagcgttcg
     1381 ctgagcggaa cgttcgtctg ttcgctcgta ttgatctgcc caaatgtcgt tttgatcgcc
     1441 agagccggta tccatccgcg aatatcgacg acgggcaggt acatgttcgc ctgcacggtg
     1501 tgtttgcggt aatcgacgtc gccaccccat ggaccgcctg cgtgctggaa atcgtacgcc
     1561 agatatgttc cggcggtggg gaacaacggg aggttccgtg aatcgcgcga atagcccaga
     1621 ccgatactcg aactgatgag cgacggattg cttggatcct gcacgtaata gtacttgtcg
     1681 gcatcggtga aatacgacgg gaaggtgtat ctctcgttgc ggaagctgta gctggcgtaa
     1741 acgcgcgagt agtcgtccgg ccaacggagc cgtcgtccga gtcgcagcga gaatcctcga
     1801 gtctcttccg tgaca
//
LOCUS       DSCC01000181            1621 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1051256, whole genome shotgun sequence.
ACCESSION   DSCC01000181 DSCC01000000
VERSION     DSCC01000181.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1051256"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            34..>1621
                     /gene="tkt"
                     /locus_tag="ENN56_02280"
     CDS             34..>1621
                     /gene="tkt"
                     /locus_tag="ENN56_02280"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDS74343.1"
                     /translation="MDKAKREQHINTIRFLAVDAINKANSGHPGMPMGCAPMAYALWT
                     EAMRYNPRNGDWFDRDRFVLSAGHGSMLLYAMLHLTGYPVSIDDLKNFRQWGSPTAGH
                     PEKGDAPGIETTTGPLGQGFATGVGMALAEAHLAARFNRPGHEIINHYTYAITSDGDL
                     MEGVASEAASLAGEWGLGKLIYLYDDNGITLAGETDLVFREDRTKRFEAYGWQVLSVE
                     DGNDVDAILAAIEEAKAETNKPTLIRVKTVIGYGSPKLQGTSGVHGSPLGAEEAKAAK
                     EHLGWPAEPMFHVPEDSRDAFLQAIPRGRAFEIDWQERFNAYEKAYPELAAELKLMID
                     GDLPEGWDKDIPTFKADAKGEATRKSSGIVLNAIAKNVTNVIGGSADLNPSTNTAMKG
                     LGDFVNPENSAKPPVEGAVGGEWGYAGRNVAFGVREHAMAAIGNGIAAHGGLIPFGAT
                     FFVFSDYLRPALRLAAIMGLQNIFVFTHDSIALGEDGTTHQPIEQLVSLRAMPDVTII
                     RPCDANETAEAWRVAIETRNR"
BASE COUNT          361 a          465 c          510 g          285 t
ORIGIN      
        1 ctatccctct gaagacaata ggagaatgaa ggtatggaca aggcaaagcg ggaacagcat
       61 atcaatacga tccgcttcct cgcagtcgat gcgatcaata aagccaacag cggacacccg
      121 ggtatgccca tgggttgcgc accgatggca tatgcgctct ggaccgaagc aatgcggtat
      181 aatccgcgga acggcgactg gttcgatcgc gaccgtttcg tgctctcggc ggggcacgga
      241 tcgatgctgc tctacgccat gctgcatctc accggctacc cggtgtcgat agacgatctg
      301 aagaacttcc gtcagtgggg tagtccaaca gccggccacc cggaaaaggg cgatgcgccg
      361 ggtatcgaaa cgacaaccgg accgctcggg cagggattcg ctaccggtgt cggcatggcg
      421 ctggccgaag cgcacctcgc ggcgcggttc aatcggccag gccatgagat catcaatcac
      481 tacacctacg ccatcacctc cgacggcgac ctcatggaag gcgtagcctc ggaagcggcg
      541 tcacttgccg gtgagtgggg tctcggtaag ctcatctacc tctacgacga caacggaatc
      601 acgctcgccg gcgagacgga tctcgtcttc cgcgaagatc gcacgaagcg attcgaagcg
      661 tacggctggc aggtgctcag tgttgaggat ggaaacgacg tcgacgcgat cctcgccgcg
      721 atcgaggaag cgaaggcgga aacgaataag ccaacgttga ttcgcgtgaa gacggtcatc
      781 ggctacggct caccgaaact ccagggaacg agtggtgttc acggctcacc gcttggtgct
      841 gaagaagcga aggccgcgaa agagcatctc ggttggccgg ctgagccgat gttccatgtg
      901 ccggaggatt cgcgcgacgc tttcctccag gcgattccac gcggtcgcgc gttcgaaatc
      961 gactggcaag agcgcttcaa cgcgtacgag aaggcgtatc ccgagcttgc ggccgaattg
     1021 aagctgatga tcgatggaga tctgcctgaa ggttgggaca aggatatccc gacattcaaa
     1081 gcggacgcga agggtgaagc gacgcggaag tcctccggta tcgttctcaa cgcgatcgcg
     1141 aagaacgtga cgaacgttat cggcggatca gccgacttga atccgtcgac aaacaccgca
     1201 atgaagggcc tcggtgactt cgtcaatccg gagaactccg cgaaaccgcc ggttgaaggt
     1261 gctgtgggcg gtgaatgggg ctatgccggg cgaaacgtgg cgttcggcgt ccgtgagcac
     1321 gcgatggcgg cgatcggcaa cggaattgcc gcacatggcg gcttgattcc gttcggcgca
     1381 acgttcttcg tcttcagcga ctacctccgt cctgcgctgc ggcttgcggc gatcatggga
     1441 ttgcagaaca tcttcgtgtt tacgcacgac agtatcgcgc tgggcgaaga cggcacgacg
     1501 caccagccaa tcgagcagct cgtaagcctg cgcgcaatgc cggacgtcac gatcattcgc
     1561 ccgtgcgacg cgaacgaaac agccgaagcg tggcgcgttg ccatcgagac gcggaaccgt
     1621 c
//
LOCUS       DSCC01000182            1868 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042286, whole genome shotgun sequence.
ACCESSION   DSCC01000182 DSCC01000000
VERSION     DSCC01000182.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042286"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..349)
                     /locus_tag="ENN56_02285"
     CDS             complement(<1..349)
                     /locus_tag="ENN56_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS74344.1"
                     /translation="MRVFTPDTLFLARPSVAYAETFIDALREFHNEGYFAYQNIDEIA
                     ADFPAFVDQLLADETRDATADRVPETYYWLIRRDRDTDTFVARGSIRHELTEHLRHIG
                     GHIGYAVRPSERRK"
     gene            complement(357..1052)
                     /locus_tag="ENN56_02290"
     CDS             complement(357..1052)
                     /locus_tag="ENN56_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose utilization protein ThuA"
                     /protein_id="HDS74345.1"
                     /translation="MKSRRKEYSVEKIRVLAWSERTEPDSVYPKGINGAIADGLRSHD
                     DFEVSTSSLKDVDQGLGDDRLDASDVLIWWGHNKHDLVSNERAESIARRVRAGSLGLI
                     VVHSAILSKPFKALMGTSCGIAGWREDDEPEHVHVVMPNHPIAKGVHDFTIPRTEMYS
                     EPFDVPAPDVLVLESRYEGGEYFRSGCCWLVGNGRVFSFSPGHETLPIMMQDEVKTVL
                     SNAVRWVANLAAE"
     gene            complement(1072..1740)
                     /locus_tag="ENN56_02295"
     CDS             complement(1072..1740)
                     /locus_tag="ENN56_02295"
                     /inference="COORDINATES: protein motif:HMM:PF06452.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74346.1"
                     /translation="MSNLPLYVCRRATDKIVVDGRLDEPTWDRAETFSLRMTNTGAEP
                     RLRTDVRALWDDTYFYVAFHCSDNDIWANMTEHDAPLWEEEVVEVFLDPAMLGTAYFE
                     LVVNPLNVLTDVFVVNLGNRERVFQPLRGWECEGIQHAVDVDGNPSDPDSKDTSWTVE
                     MAIPFEQLVTAPNIPPKVGDTWRGNFYRIEQNAEGDEYTAWSPTGAINYHISDAFGTI
                     VFAE"
BASE COUNT          413 a          571 c          477 g          407 t
ORIGIN      
        1 ctttccgccg ctctgaagga cgcaccgcgt acccgatgtg cccgccgata tggcgcaaat
       61 gctctgtgag ttcgtgccga atcgacccac gcgcgacaaa cgtatccgtg tcgcgatcgc
      121 gacgaataag ccaatagtac gtttcaggaa cacggtcagc tgttgcatcg cgagtttcgt
      181 ctgcgagaag ttgatcgaca aatgctggaa agtctgctgc gatctcatcg atattctggt
      241 aagcaaaata cccttcgttg tggaactcac gaagcgcatc gatgaatgtc tcggcatacg
      301 cgacggacgg tcgcgccaga aagagtgtat caggcgtgaa aactcgcatc aatcactcac
      361 tcagcagcca gatttgcgac ccaacgtact gcattggaga ggacggtttt cacctcatcc
      421 tgcatcatga tcggcaacgt ctcgtgtccc ggactgaagg aaaacacgcg gccgtttccg
      481 acgagccagc aacaacccga ccggaagtat tcaccgcctt catagcgtga ttccagcacg
      541 agaacgtcgg gggccggcac gtcgaagggt tcgctgtaca tctcggtccg cggaatcgta
      601 aagtcgtgga cgcccttcgc gattggatga tttggcatca ctacgtggac gtgctccggc
      661 tcatcatcct cccgccaacc agcgatacca cacgatgtgc ccataagcgc cttgaacggc
      721 ttcgacagaa tcgccgaatg cactacgatc aacccgagcg agcccgctcg cacgcgccga
      781 gcgatgcttt ccgcacgttc attgctgacg agatcgtgtt tgttgtgacc ccaccagatc
      841 agcacgtccg acgcgtcgag tctgtcatct ccgaggcctt gatccacgtc tttcagggaa
      901 ctcgtcgaaa cctcgaagtc atcgtgtgaa cgcaatccat ccgcgatagc cccattgatt
      961 ccctttgggt acaccgagtc gggttctgtc cgctccgacc atgcgagaac gcgtatcttt
     1021 tccacgctat actccttccg ccgactcttc agtcacgtcg tgatctgcgt tttactccgc
     1081 gaacactatc gttccgaacg cgtcagagat gtggtagtta atcgcgccag ttggtgacca
     1141 tgcggtatat tcgtcgcctt cggcattctg ctcgatgcga taaaagtttc cacgccacgt
     1201 atcgccaact ttcggcggaa tattcggcgc ggtcaccaac tgttcgaacg gaatcgccat
     1261 ctccaccgtc cacgacgtgt ctttgctatc gggatccgac gggttcccgt cgacatcgac
     1321 tgcgtgttga atcccctcac attcccaccc gcgtagtggc tggaaaacgc gctcacgatt
     1381 gccgaggttc accacgaaga catccgtcag gacgttcaac gggttaacaa ccaactcgaa
     1441 atacgccgtg ccaagcatcg caggatcgag aaacacctcg accacttcct cttcccagag
     1501 cggtgcgtcg tgctccgtca tattcgccca gatatcgtta tccgagcagt ggaatgcaac
     1561 atagaagtac gtatcatccc ataacgcccg gacgtcggta cgcaagcgcg gctcggcacc
     1621 tgtattggtc atgcgtaacg agaacgtctc tgcacgatcc catgtaggtt cgtcaaggcg
     1681 cccgtcgaca acgatcttgt ccgttgcgcg acgacatacg taaagcggca gattcgacat
     1741 cgtaacctcc gatttgtcgt tagagaacac gtgtacgcga gtccggcgcg cgaaaccggt
     1801 accgaagtgc aaccctgcgc cgtgctgtcg gatactcacc ttgcgccgcg aattcgtcgg
     1861 tatgtagc
//
LOCUS       DSCC01000183            1578 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053107, whole genome shotgun sequence.
ACCESSION   DSCC01000183 DSCC01000000
VERSION     DSCC01000183.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053107"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            208..513
                     /locus_tag="ENN56_02300"
     CDS             208..513
                     /locus_tag="ENN56_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDS74347.1"
                     /translation="MTHELDRVNHLAIPVSDIAKAVEWYAARFRCDVVYQDETWAMVR
                     FENLKIAFVLPKQHAGHVAFEVDDLEAFGEVKTHRDGVRYVYLDDPFGNTVEVVPKE"
     gene            604..888
                     /locus_tag="ENN56_02305"
     CDS             604..888
                     /locus_tag="ENN56_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74348.1"
                     /translation="MYQVGTWMILATAVLLLVLTLGVSRFAPKLVLPALIITWTLGVV
                     GFVLVCIAEFTTANIILAVLFIAAGIAHGIRVKRRADARSTQPPSDAQNT"
     gene            916..>1578
                     /locus_tag="ENN56_02310"
     CDS             916..>1578
                     /locus_tag="ENN56_02310"
                     /inference="COORDINATES: protein motif:HMM:PF13625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDS74349.1"
                     /translation="MNDTERPLIVQSDRSILGEVDSPGYAEARDALAQFAELEKSPEH
                     IHTYRITPLSLWNAAASGLDADSIVAALRRFSRYDVPENVISDITEFIGRYGRVRLEA
                     VDDELHLVSSDLPLMAELANHKQIAPMLVRRVDTGRYAIARYSRGHIKQALIRLGFPV
                     EDLAGYVEGAHLPIDLKTALPSGEPFTIRQYQREAADSFWAGGSTRGGSGVVVLPCGA
                     GKT"
BASE COUNT          335 a          447 c          464 g          332 t
ORIGIN      
        1 aatcacacgt tcgcgaagcc ggttgtgaac gcgcatcccg atttcgccgt actctgggcc
       61 gacgggcacg ggcgtgagga atctccgtca cgactgtact tcaccgaccg cgacgggatg
      121 aatgtgtggc ggctcccgac aaccatgtcg tcggatttcg cggaacccga gctgattggg
      181 gtctgatatg ccgagttgga gagagaaatg acgcacgaat tggaccgggt aaaccacctt
      241 gcgatcccgg tctcagatat cgcaaaggcg gtagagtggt acgcagccag atttcgatgc
      301 gacgtggtgt accaagacga aacgtgggcg atggtgcggt ttgaaaactt gaagatcgcg
      361 tttgtgcttc cgaagcaaca cgccggacac gttgcatttg aggtcgacga tctcgaagcg
      421 ttcggcgaag taaaaactca ccgcgacggc gtccgctacg tctacctcga tgacccgttc
      481 ggcaacacag ttgaagttgt accgaaggag tagccgccac ggttcgatct cgttgacgta
      541 catgcgactt ccggtgtaca ctctctggac gtacgcgaac cgttcgcaga aaggaagtgc
      601 gcgatgtacc aagtcggaac gtggatgatc ctcgcaacgg cggtgttgct gctcgtgctc
      661 acactcggcg tttcacggtt cgcgccgaaa ctcgtccttc ccgcgctgat aatcacgtgg
      721 acactcggtg ttgtcggttt cgtattggta tgtattgcgg aatttacgac tgcgaatatc
      781 atccttgcgg tgttattcat cgcggccggt atcgcacacg gcattcgcgt aaagcgacgc
      841 gccgacgcac gaagcacaca accgccgtcc gacgcgcaga acacatgatc tcaatctatc
      901 tggttatctc cacatatgaa tgacactgaa cgcccgctca ttgtgcaaag tgaccgctcg
      961 atactcggtg aagtggatag tcccggctat gcggaagcgc gagatgctct cgcgcaattc
     1021 gccgagctcg agaagagccc ggaacacatc cacacctacc gcattacccc gctttccctt
     1081 tggaacgccg ccgcaagtgg tctcgatgcc gatagtatcg tagccgcgct cagacgattc
     1141 tcgcgctacg acgtgccgga gaacgtgatc tcggatatca ccgagttcat cgggcggtac
     1201 ggtcgtgtgc gtcttgaagc cgtcgacgac gagctgcatc ttgtatcgag tgatctgccg
     1261 ctgatggcgg aacttgccaa ccacaagcag atcgcgccga tgctcgtacg acgtgtcgat
     1321 acgggacggt acgcaatcgc gcggtacagt cgcgggcaca tcaagcaagc gctcatccgt
     1381 ctcggttttc ccgtcgagga cctcgcggga tacgtcgaag gcgcgcacct tccgatcgat
     1441 ctgaaaaccg cacttccgag tggtgagcca tttacgattc gacagtatca gcgcgaagcg
     1501 gctgattcgt tctgggcggg aggttccacg cgaggtggca gcggcgtcgt tgttctgccg
     1561 tgcggtgccg gaaagacc
//
LOCUS       DSCC01000184            1537 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055013, whole genome shotgun sequence.
ACCESSION   DSCC01000184 DSCC01000000
VERSION     DSCC01000184.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055013"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..374
                     /locus_tag="ENN56_02315"
     CDS             <1..374
                     /locus_tag="ENN56_02315"
                     /inference="COORDINATES: protein motif:HMM:PF10633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74350.1"
                     /translation="QVWPYEQGIEPGKPFSATVKLRNHERASANGTITAALPDGWKAD
                     HPVQKVHIHGGGSSEVTFTIVPGERRAGAPGKVAYGFALELNGKPLGEACHGIGNYLR
                     YPYLAHETVHHHGAHAHDHAE"
     gene            391..>1537
                     /locus_tag="ENN56_02320"
     CDS             391..>1537
                     /locus_tag="ENN56_02320"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74351.1"
                     /translation="MPDRPNIVVIETDSQDGRVLGCMGHPSMRGADGSSLTPNLDALA
                     ARGMLFRQTYSNNPICCPSRSSMVSGQYTHHCEGWNNYKGLEPDDETFFDRLADTGYR
                     TQIFGKTDWLSGYHTIRARVSPWTRSAWIMRANYRMDPPQVEDSDERRYHTRDWDDVD
                     RATAWLGDEGTSTDAPFLLYLGIRQPHPAFRTTRYWYDRVDPDSIEIPRLGSVDGHPA
                     LKYQRVNKNWMHGTDEDTVRLVRRVYFAQIAEVDDMVGTVLGALDETGLREDTYVLFL
                     SDHGEMAMEHGQFYKTSHFEPSVRVPLIIAGPDVRPGTETNALTTLVDIHPTLMDMAG
                     APQSEKADGNSLLPELRGAPSRRPNYALSENHESSLPTGSFMFRSDID"
BASE COUNT          332 a          472 c          466 g          267 t
ORIGIN      
        1 cacaagtctg gccgtacgaa caaggcatcg aaccggggaa gccattcagt gcgacggtga
       61 agctgaggaa ccatgagcgc gcgtcggcaa acggcacgat caccgcggcg cttcccgacg
      121 gctggaaagc cgatcatccg gtgcagaagg tgcacatcca cggtggcggc tcttcggagg
      181 tcacgtttac gatcgtaccg ggagagcgtc gcgccggcgc gccggggaag gtcgcgtacg
      241 gcttcgcgct ggagctgaac ggaaagccgc tcggtgaggc gtgccacggc atcggtaact
      301 acctgcgcta tccgtatctc gcacacgaaa cggtacatca ccacggtgcg cacgcgcacg
      361 accacgcgga gtaaatcgga ggatttcgat atgcctgatc gcccaaacat cgttgtgata
      421 gaaaccgaca gtcaggacgg tcgtgtactc gggtgcatgg ggcacccgtc gatgcgcggc
      481 gcagacggct cgagtttgac accgaacctc gatgcactcg ccgcgcgcgg gatgctcttc
      541 cggcagacct actcgaacaa cccgatctgt tgcccgtcgc gctcatcgat ggtgagtggg
      601 cagtatactc accactgcga gggatggaac aactacaagg gactcgaacc cgacgacgaa
      661 acgttcttcg accgtcttgc ggataccgga tatcgaacgc agatcttcgg aaaaaccgat
      721 tggctctccg gataccacac gatccgcgca cgcgtctcgc cttggacccg gtccgcgtgg
      781 attatgcgcg cgaactaccg catggatccg ccgcaggtgg aggattccga cgagcgtcgg
      841 taccacacgc gcgactggga cgatgtcgac cgcgcaacgg cgtggcttgg cgacgaaggg
      901 acatcgaccg acgcgccgtt tttgctgtat ctcggcatcc gacagccgca tccggcgttc
      961 cggacgactc gatattggta cgatcgcgtc gatcccgact ccatagaaat cccgcgcttg
     1021 ggatcggttg acgggcatcc cgcgttgaaa taccaacggg tgaacaagaa ttggatgcac
     1081 ggaaccgatg aagacacagt tcgtctcgtt cgacgcgttt attttgcaca gatcgccgag
     1141 gtggacgaca tggtcggcac ggtgctcggc gcactcgacg agacgggatt gcgggaggac
     1201 acgtacgtgc tgtttctctc cgaccacggc gaaatggcca tggagcacgg acagttctac
     1261 aaaacctccc acttcgaacc atccgtacgt gtgccgttga tcatcgccgg tcccgatgtc
     1321 cgacccggta cggaaaccaa cgcgctgacg acactggtgg atatccatcc gacgctgatg
     1381 gacatggcgg gagcgccgca atccgaaaag gccgacggaa actcgttgct gcccgaactc
     1441 cgcggcgcac cgtcccggcg tccgaactac gcgctcagtg aaaaccacga gagctcccta
     1501 ccgaccggat cgttcatgtt ccgatccgat atcgacg
//
LOCUS       DSCC01000185            1880 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1041917, whole genome shotgun sequence.
ACCESSION   DSCC01000185 DSCC01000000
VERSION     DSCC01000185.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1041917"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            346..1347
                     /locus_tag="ENN56_02325"
     CDS             346..1347
                     /locus_tag="ENN56_02325"
                     /inference="COORDINATES: protein motif:HMM:PF01408.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74352.1"
                     /translation="MAIRLGFIGVGGIAGTHLNAAKALGIPVSAMFDVSRETMEKRQQ
                     EFGIPHICASAKELAEHPEVDAVIVASPQHVHLEGIRAACKAKKPLFTEKPLARTLEQ
                     AHEAVRLVEEAGIVAQVGFVRRYCLEWGAFRSAIESGVIGSPVVWWMAGGGPGPRSPF
                     FMTHDQGGGPMLDGMVHNYDFCRYIWGEPTKVIGSMVNLRPDTDALDTGTAIIEFAGG
                     HRHTILNSWGLPEGTRSGGFHNVLGPKGVFYFGDPDNNPPDDLDTKTHGYHVSKLAGG
                     ERKVHLYEKRNMYEAQLADFVQKAEKGDRKTRATIKDGLKAQEIALAILGEFTPGQR"
     gene            1470..1835
                     /locus_tag="ENN56_02330"
     CDS             1470..1835
                     /locus_tag="ENN56_02330"
                     /inference="COORDINATES: protein motif:HMM:PF06249.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDS74353.1"
                     /translation="MATAKPTDEKFIIRQLKDLAEARSTCGFRRALFTREDSDRISMS
                     ILRIDNSQKHWHNETHEIYYVLDGEGSLELDDDEVPLSPGVAVLVKPGVRHSAKGEVT
                     VLIVGAPPFSQEDIFIEEK"
BASE COUNT          414 a          519 c          555 g          392 t
ORIGIN      
        1 gcacttcgac gatacgttcc ttcgtaaaat acccttttag ccacgtatca cgggcatgac
       61 taaagtgcga gaatccgtta aacgggttcg ttccacatgt tacacgggat acgtcaattc
      121 cgccgatatt cgtcatggga agcggtgtca tgcgcgttct ccttatcggg taaagtgccg
      181 cgagcctgat cgcacgaaaa tcctgatgtc tgatacgata gattcggtaa cgattcggtc
      241 aaagccgtga cgagttcacg ttgtgccgtt tcccccgatt cagatcgcgc gtacctttat
      301 cactctgtca attcgcatcc acactactgt caaggagtcg cacgtatggc cattcggctc
      361 ggattcatcg gtgtcggcgg tatcgccgga acgcacctca acgcggcgaa ggcgctcggc
      421 attcccgtga gcgccatgtt cgatgtcagc cgcgaaacca tggagaaacg gcagcaggag
      481 ttcgggattc cgcatatctg cgcgtccgcg aaggaactcg ccgaacatcc ggaagttgac
      541 gccgtcatcg tcgcctcgcc gcagcatgtt catcttgaag gcattcgtgc ggcgtgcaag
      601 gcgaagaaac cgctctttac cgaaaaaccg ctcgctcgca cgctcgaaca ggcgcatgaa
      661 gcggtgcgtc ttgtggaaga agcgggtatc gtagcgcagg tcggttttgt tcgccgctat
      721 tgtctggaat ggggcgcatt ccggagcgcg atcgagtcag gagtcatcgg cagcccggtt
      781 gtctggtgga tggctggtgg cggaccgggt ccgcgcagtc cgttcttcat gacacacgat
      841 cagggcggtg gcccgatgct cgacggcatg gtgcataact acgatttctg ccgctacatc
      901 tggggcgagc cgacgaaggt cataggatcg atggtgaacc tacgcccgga taccgacgcg
      961 ctggatacgg gcacggcaat aatcgagttc gccggtggac atcgccatac gattctgaac
     1021 tcgtggggac tgccggaagg aacgcggtcg ggcggattcc ataatgtgct cggacccaaa
     1081 ggcgtattct attttggcga tccagataac aacccgcccg acgacctcga tacaaagacg
     1141 cacggctacc acgtctcgaa actcgccggc ggcgagcgaa aagtgcacct gtacgagaaa
     1201 cggaatatgt acgaggcgca gctcgccgat ttcgttcaga aggcggaaaa aggcgatcgg
     1261 aaaacgcgcg caacgatcaa agacggtctc aaggcgcagg agatcgctct cgcgattctc
     1321 ggtgagttta caccgggtca gcgttgaaaa acgtggcgaa ctcggtgcgg tgcgccgggt
     1381 tcgcctcttt gtttgactgg tcgccattcc gcagtaccat tcctctacgc acgatcgatc
     1441 aacgcgggac gtatgggaga ttcccctcga tggcaactgc gaaacccact gacgaaaaat
     1501 ttatcatacg acaactcaag gatctcgctg aagcgcgctc tacatgcgga tttcggcgcg
     1561 cgctctttac gcgtgaggat tccgaccgca tctcgatgtc gatactccgg atcgacaact
     1621 cacagaagca ctggcacaac gaaacgcacg agatctacta cgttctcgat ggtgaggggt
     1681 cgctcgagct cgacgatgat gaggtgccat tgtcgcccgg cgtggcggtg cttgtcaagc
     1741 cgggtgttcg acattcggcg aagggcgagg tgacggtgct catcgtcgga gcaccgccgt
     1801 tttcgcagga ggatattttt atcgaggaga aatgaacgcg cgcaagtaac gcgccgtcac
     1861 cggaacagtg tgttccgtcc
//
LOCUS       DSCC01000186            1723 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047133, whole genome shotgun sequence.
ACCESSION   DSCC01000186 DSCC01000000
VERSION     DSCC01000186.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047133"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..280)
                     /locus_tag="ENN56_02335"
     CDS             complement(<1..280)
                     /locus_tag="ENN56_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74354.1"
                     /translation="MRLLLPFKVLLTICCAVIVSTVPVRAVLLTSPDVRVDLVAETAL
                     VFWQDGIQHIVLQPVITAGDIDRQPTRVAYVVPVPSVPTSYGATDDAII"
     gene            450..>1723
                     /gene="lysS"
                     /locus_tag="ENN56_02340"
     CDS             450..>1723
                     /gene="lysS"
                     /locus_tag="ENN56_02340"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:PRK00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDS74355.1"
                     /translation="MEYRNQLIAQRVAKLEKLREMGVEPYPYRYDPTHSVAEILADVD
                     AFLEKPVRIAGRLVAIRDMGKAAFAHVQDSAGRIQIYVRRDTVGEAVYDAWKQLDIGD
                     IVGLSGPIMRTRTGEVTVQVFELTVLAKSIRPLPVVKEREVDGKRETYDDITDNIELR
                     YRHRSIDLTVNPATREVFRKRATILRTVRRVLDERGFVEVETPTLQPLYGGANARPFK
                     THHNALDVDLFLRISPELYLKRLVVGGLDRVYDLSKNFRNEGIDRTHNPEFTMLEVYQ
                     AYADYTDMMELTETLYAEAAKAVVGTTKIVYQGRDIDLTPPWERLTMIDAIARYADID
                     IRALDDSSLGKLVSERDPDFEVGTATRGTLINELFEQCVEEKLVGPVFITEHPLETTP
                     LCKQSRTIPGHIERFEPYIAGWEIGNAYSELN"
BASE COUNT          407 a          444 c          521 g          351 t
ORIGIN      
        1 cgataatcgc gtcatcggtg gcgccatacg atgtcggtac tgacggaacc ggaacgacgt
       61 acgccacgcg agttggttgc cgatcgatgt cgcccgcagt gatcactggc tggagaacga
      121 tgtgttggat tccgtcctgc cagaatacaa gcgccgtttc cgcgacaaga tcgacgcgta
      181 cgtctggaga agtgagtaac acggcacgaa ccggtaccgt cgatacgatc acggcgcagc
      241 atatggtcag aagaaccttg aatggcagaa gaagcctcat atatcctccg ttgagcagcg
      301 gtatcggcgt atccgagggt agcgcgccgg ttgtgaagta tgatgcggaa gcggtacatt
      361 aacaaaggta ccgcgcgttt gttttcgaag cgcgccaaac tcactcctgc gtaggtttcg
      421 cgcaaacccc ttgttcccga ggaattccgg tggagtatcg aaatcaattg atcgcgcaac
      481 gtgtcgcgaa gctggaaaag cttcgtgaaa tgggtgtcga gccgtatccg taccgatatg
      541 atccgactca tagcgtcgca gagatcctcg cggatgtcga cgcgtttctg gaaaaaccgg
      601 tgcgtatagc aggtaggctc gtcgctatac gtgatatggg caaggcggcg tttgcgcatg
      661 tacaagacag tgccggacga atccagatct acgttcgtcg cgatacagtc ggcgaagcgg
      721 tgtacgacgc ctggaagcag ctcgatatcg gcgatattgt gggactttcc ggaccaatca
      781 tgcggacgcg caccggcgag gtaacggtcc aggtatttga gctgacggtc ctcgcgaaga
      841 gtattcgtcc cctgcccgtc gtaaaagaac gtgaagtcga tgggaaacgc gagacgtacg
      901 acgacattac ggataacatt gagctacgat atcgccaccg atccattgat ctgaccgtga
      961 atccggcgac gcgcgaggtg tttcgcaagc gggcgacgat tcttcgaaca gtgcggcgcg
     1021 ttctcgatga gcgcggattc gtcgaagtcg aaacgccaac gctgcagccg ttatacggtg
     1081 gcgcgaacgc tcgtccgttc aagacgcatc acaacgcgtt ggatgtcgat ctcttcctgc
     1141 ggatcagccc ggagctctac ctcaagcggc tcgtggttgg agggctggac cgggtgtacg
     1201 acctgtcgaa aaactttcgc aacgaaggca tcgacagaac gcacaatccg gagtttacga
     1261 tgttggaggt gtatcaggcg tacgccgatt acaccgatat gatggagctg accgagacgc
     1321 tctatgccga agcggcaaag gcggtcgttg ggactacgaa gatcgtgtat caaggtcgcg
     1381 acatcgattt gacgccgcca tgggaacgac tgacgatgat cgatgcgatc gcgaggtatg
     1441 ccgatatcga tattcgcgca ctcgatgata gctcgctcgg gaaactggtt tccgagcgtg
     1501 atccggactt cgaggtcggc accgcgacac gcgggacctt gatcaacgag ctcttcgaac
     1561 aatgcgtcga agagaagctc gtcggacccg tgtttattac cgagcacccg ctggaaacga
     1621 caccgttgtg taaacagtcg cgcacgatac cgggacatat tgaacggttt gagccatata
     1681 tcgccggatg ggaaatcgga aacgcgtact cggagttgaa cga
//
LOCUS       DSCC01000187            3544 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017832, whole genome shotgun sequence.
ACCESSION   DSCC01000187 DSCC01000000
VERSION     DSCC01000187.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017832"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            744..1112
                     /locus_tag="ENN56_02345"
     CDS             744..1112
                     /locus_tag="ENN56_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HDS74356.1"
                     /translation="MHADYLPVLFMLALAVATAGGIVVATHFLGPRKPTYYKTQVPYE
                     AGADPVGSARLRFDVRFYLVALLFVVFDVEVVYFYPWAVVFRDLIQQNLTALWAILIF
                     SFIVFIGLVYEWKKGALDWK"
     gene            1270..2409
                     /locus_tag="ENN56_02350"
     CDS             1270..2409
                     /locus_tag="ENN56_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74357.1"
                     /translation="MWISELRFQDPRTKTYLGSPSITRLPDGALIGTHDYFGAGSPHN
                     MEDEEHLTSVYRSEDNGITWRTVTHVSGAYWSTVFVHNGALYLFGASAQYGSMVIRRS
                     DDGGYSWTYPVDENTGLISRGGPYHEPPNYHCAPVPVLVHNGRIYRAFEDDSRIDYGR
                     DFLAVVVSAPVDADLLKAENWTISNKLKFEGAKAPQYSGNPTWLEGNIVADPEGQLWN
                     VLRFNAGSKDDPVFNTAAFVKIDDDGKKLSFDYETGFRHLPGGATKFTIRHDPVSNLY
                     WTMVNEQQHEPYPLRRNRLSLFTSPDLGEWTRRKILLEDNLEAIPENSIRNTGFQYVD
                     WIFDPAEDDTLLYLVRTAYDGAHNFHDANRITFSRVRNFRHLVTK"
     gene            2573..3169
                     /locus_tag="ENN56_02355"
     CDS             2573..3169
                     /locus_tag="ENN56_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HDS74358.1"
                     /translation="MDMARKTATDKMNGEGFLTTRIDELVKWGRKNSIWPMPFGTACC
                     GIEMMAVLGPKFDLARFGAEAIRFSPRQSDMLIVAGRVSIKMMPVLQRIYEQMPEPKW
                     VVSMGACASCGGVFNTYTLIQGIDQFIPVDVYVSGCPPRPEALIDAVMEIQRIIEQGE
                     DAVFAFKLGKKKIEVGPPPADDSIYARETKGLHLPVMR"
     gene            3196..>3544
                     /locus_tag="ENN56_02360"
     CDS             3196..>3544
                     /locus_tag="ENN56_02360"
                     /inference="COORDINATES: protein motif:HMM:PF00329.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HDS74359.1"
                     /translation="MFPAARRRRHMSQQIAEKIRDQFPDGVYGISEHAGKWRVDIHRE
                     ANLQILRWCYDELGMTYLADVTCVDLLDMPIEAPARFEVIYVLRNLGAREYIVLRAYV
                     PEDDPTIDSATAIW"
BASE COUNT          738 a          974 c         1050 g          782 t
ORIGIN      
        1 ggataaacgc cgatggttca tgcggcaggg caaaacagga tattggctga gtaatgccga
       61 ccttctgcac cctcatatcg cctttatttg tctgacgagt atttcggtgt tttgccgtta
      121 gccgcttcaa caaccggtcc cttccgacaa ggaccgtatc agaatgatgc acgctgtcgc
      181 agaacacgaa acagtgtgta acagaccgcg cgagactgta acacgccacg gtacggcgcg
      241 ggatgcggtc ggttccatgt cgaacgtcac aggacggccg atgtcccgat tgcggcctga
      301 acgtttcgag acgaaaaacg ccgaccgaag ccctatctgc atcccatgaa taggtttgag
      361 ccttcagctt ctaccgtacg ccacgtgtgt ggtacaacta ttgctctgtt tttcccgtag
      421 gtataccgcg caggtgcgga atcgaatctt gcggcgggaa ccaaagaaac atggtgcgca
      481 ttgaattaca agaatacgac acggtgttta tgatcctcgt gtgatagacg gtcctcctcc
      541 cgactatcac atagtatccc ggtcggttcc tcccccttga ctgaccggga tccccttctg
      601 tgacagggcg cggatgaaaa ttcgcgccct tgttgttttc tgtaacgctt cgtcacgtcg
      661 gttggcggcg agtccttgcc gattcagttt tcgtacgcta cttttccgtg ccacaaacac
      721 aaaaatggtg atgcggagga ccgatgcacg ctgactatct gccagttctg ttcatgcttg
      781 cgctcgcggt ggcgactgcc ggcgggattg tcgtcgcgac gcattttctt gggccgcgga
      841 aaccgaccta ctacaagacg caggtgccgt acgaagccgg cgcggatccg gtcggttcgg
      901 ctcggctcag gttcgatgtg cggttttacc tcgttgcgct gctcttcgtc gtattcgatg
      961 tcgaggttgt ctacttctat ccgtgggcgg tggtcttccg cgatctcatc cagcagaacc
     1021 tcaccgcact ctgggcgatc ctcattttct cgttcatcgt attcatcggc ctcgtgtacg
     1081 aatggaaaaa gggcgcgctc gactggaaat agccggtgta tcgcgcgaaa cgaaagtgaa
     1141 accggcgtct cgatcaactc gagcgccggt ttcttgtgta cggtgcggtt tccggtagcg
     1201 tccgcgccgt gatattccta ccttttgctt tccgatgctt tccatcgcgt gagaaggaga
     1261 cgtttcgcca tgtggattag cgaactgcgg tttcaggatc ctcgcacgaa aacctacctc
     1321 ggcagcccca gcatcacgcg cctgcccgat ggcgcgctga tcggcacgca tgactacttc
     1381 ggcgccggtt cgccgcacaa catggaagac gaggagcacc tcacgagtgt gtatcgcagc
     1441 gaagacaacg ggatcacgtg gcgcactgtc acgcatgtct ccggtgcgta ctggtcgacg
     1501 gtgttcgtac acaacggcgc gctctacctt ttcggcgcat cggcacagta tgggagtatg
     1561 gtgattcggc gctccgatga cggcgggtat tcgtggacct accccgtcga cgagaacacc
     1621 ggattgatct cgcgtggcgg accgtatcac gagccgccca actaccactg cgcgccggtt
     1681 ccggtgctcg tgcacaacgg tcgcatctat cgcgcgtttg aagacgacag tcgaattgac
     1741 tacggccgag attttctcgc ggtcgtagtc agcgcaccgg ttgacgcgga cctgctgaaa
     1801 gcggagaact ggacgatctc gaataagctg aagttcgaag gggcgaaagc gccacagtat
     1861 tcggggaacc cgacgtggtt ggagggtaac atcgtcgccg accccgaagg gcaactgtgg
     1921 aacgtgttgc ggttcaatgc cggatcgaag gatgatccag tgttcaatac cgctgcgttt
     1981 gtgaagatcg acgacgacgg caagaagctg tcgttcgact acgagacggg attccgacac
     2041 cttcccggcg gtgcgacgaa gttcaccatc cggcacgatc cggtgtcgaa tctctactgg
     2101 accatggtaa acgagcagca gcacgagccc tatccgctcc gtcggaatcg actttcgctc
     2161 ttcacgtcgc ctgacttggg tgagtggacg cgtcggaaga ttctgctcga agacaacctc
     2221 gaagcgatac cggagaacag tatccggaat accggtttcc agtacgtgga ctggatcttc
     2281 gatccggcag aggacgatac gttactctat cttgtacgaa cggcgtacga cggcgcgcac
     2341 aatttccacg acgcgaaccg tatcacgttc agccgcgtgc gcaacttccg gcatctcgta
     2401 acaaaatagc gccgacacgg cgaaaagcgg cgtagcatcg gggttttgga tcggctcgtc
     2461 gttggtcctt gttggattcc ggcggtcggc ctatctttcg atttccgtgc gcgtccggtg
     2521 gtatcagaga agaaaacgac gcgtattcag agagtttttc gcatccgtag gaatggatat
     2581 ggcacgaaaa acggcaactg acaagatgaa tggtgaggga ttccttacca ctcgtataga
     2641 tgagctcgtg aagtggggcc ggaagaacag catctggccg atgccgtttg gcacggcgtg
     2701 ctgcggcatc gaaatgatgg cggtgcttgg accgaaattc gatctggcgc gattcggtgc
     2761 agaggcgatt cgattcagtc cgcggcagtc ggatatgctc attgtcgcgg ggcgggtgtc
     2821 gatcaaaatg atgccggtgc tccagcgcat ctacgagcag atgcccgagc cgaagtgggt
     2881 cgtcagcatg ggcgcgtgcg cgtcgtgtgg cggagtgttc aacacgtata cgctcattca
     2941 gggcatcgac cagttcattc ccgtggatgt gtatgtatcg ggctgtccgc cacgtccgga
     3001 agcgctgatc gacgcggtca tggagatcca acgcattatt gagcagggcg aggacgcagt
     3061 tttcgcgttc aagcttggca agaaaaagat cgaggtcgga ccgcctcctg cggacgattc
     3121 gatctacgcg cgcgaaacca aaggtctgca cctgccggtc atgcggtaac gaaacgtatt
     3181 cagtgcggat gcgggatgtt tcccgccgca cgtcggagac gccatatgag tcagcaaatc
     3241 gcggagaaaa ttcgcgatca attccccgac ggagtctacg gaatcagcga acacgcgggg
     3301 aagtggcgcg tggacatcca tcgcgaagcg aacctgcaga tccttcgttg gtgttacgac
     3361 gaactcggca tgacctacct cgccgacgtg acatgtgtcg atctcctcga tatgccgatt
     3421 gaagcgccgg cgcggttcga ggtgatttat gtgctgcgga atctcggtgc gcgtgagtac
     3481 atcgtcctgc gcgcgtacgt tccggaggac gatccgacga tcgactcggc gaccgccatc
     3541 tgga
//
LOCUS       DSCC01000188            1870 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042212, whole genome shotgun sequence.
ACCESSION   DSCC01000188 DSCC01000000
VERSION     DSCC01000188.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042212"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            248..1120
                     /gene="zapE"
                     /locus_tag="ENN56_02365"
     CDS             248..1120
                     /gene="zapE"
                     /locus_tag="ENN56_02365"
                     /inference="COORDINATES: protein motif:HMM:PF00308.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDS74360.1"
                     /translation="MGKNAPQIGCERCRPYYGQGYRLEKNVDRENPYPRVVPCECARS
                     LCVCGALRDARNVEGPNYTELSERAADAVSELDCPCWPIRQKIDRINRLLLASKVDHV
                     GIMLSDVQTKVNGRALQETQFAVGAASSFIEHAAKGEKQKGLYIYGKTGGGKTYLASA
                     LLNELIIRTARPGLFVNVTQQLFPRLRQTYEDENRSETEMQIMSQLSRMPYLVLDDLG
                     VERNTKWESEVLYNIIDVRYRRRKEVIITSNVSPDEFESLSRGRITSRLKHMCVVIKM
                     PDEDLRQYFDYVVR"
     gene            1117..>1870
                     /gene="rsmA"
                     /locus_tag="ENN56_02370"
     CDS             1117..>1870
                     /gene="rsmA"
                     /locus_tag="ENN56_02370"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase A"
                     /protein_id="HDS74361.1"
                     /translation="MNDERVWKPRKSLGQNFLVDKHIAAKIVDESDIGSGETVLEIGP
                     GRGALTGLLAERAHRVVAIEVDAVMVAHLQRHFESSNVTILHRDILDVDFAELGNEFG
                     VSRWPIIGNLPYVISSRVAMNLVAQARSIARATIMVQREVGERYLAPPGSRDYGLLSV
                     LLQATGSLSRGFRVGPGAFRPQPRIESIVLTWISDPPPDMAIQPLITTVKAAFAQRRK
                     QLRNALRGIPGASDDVIATACAQVGIDLSARAE"
BASE COUNT          425 a          493 c          560 g          392 t
ORIGIN      
        1 gcacgattcg gcgcatcgtc ggtccagagc tgcagctcac cgattcggcg gaagaggtcg
       61 cgcgcgaagt cgcagcgatg ttgaacgagc aaggactggc gagcgacgga ggcgctgcgt
      121 tcgggcaccg cttcattgtg agcgacgtcc cgcacaaatt tcgggaagtc ggcgagcgat
      181 tcctcggaca cacgcttccc acggttgaag tgaacgcgat ttcatagaca aggtaggtgt
      241 acttggcatg ggcaaaaacg cgccgcagat cggttgcgaa cggtgtcgtc cgtattatgg
      301 gcaaggctat cgactcgaga aaaacgtcga tcgcgagaac ccctatccac gggtcgttcc
      361 atgcgaatgt gcgcgatcgc tatgtgtgtg tggcgcgctg cgcgatgccc gcaatgtaga
      421 gggtccaaac tacactgagc tttccgaacg tgccgctgac gccgtgagcg agctcgattg
      481 tccgtgttgg ccgattcgtc agaagatcga tcggattaac cgattgcttc tcgcctcaaa
      541 ggttgaccac gtcggtatta tgctgagcga cgtgcaaacg aaggtgaatg ggcgagcgct
      601 gcaggagaca cagttcgcag tcggtgccgc gagttcgttt atcgagcatg cggcgaaagg
      661 tgaaaaacaa aaaggtctct acatttacgg aaagaccggt ggtggtaaga cgtatcttgc
      721 cagcgcgtta ttgaacgagc tcatcattcg gacggcgcga ccgggcctgt tcgtaaacgt
      781 cacacaacag ctgtttccgc ggttacgcca gacgtatgag gacgaaaacc ggtcggaaac
      841 ggaaatgcag ataatgagcc agctttcccg catgccgtat ctcgtactgg acgacctcgg
      901 tgtcgagcga aacacaaaat gggaatccga ggtgctctac aacattatcg acgtgcgcta
      961 ccgccggcga aaagaagtga tcattacgag taacgtgtca cctgacgagt tcgagagtct
     1021 ctcgcgcgga cgaataacgt cgcgcctaaa gcatatgtgc gtcgtgataa agatgcctga
     1081 cgaggacctt cgacagtatt tcgactacgt agttcgatga acgacgaacg ggtgtggaaa
     1141 ccgcgaaagt cacttggaca aaacttcctc gtggacaaac acatcgcggc gaaaatcgtt
     1201 gatgagtcgg acatcggctc cggcgaaacc gttctggaga tcggtccggg acgaggtgcg
     1261 cttacgggtt tgctcgctga acgtgctcac cgcgtcgtag cgatcgaagt ggacgcagtc
     1321 atggtggcac accttcaacg ccacttcgag tccagtaatg tcaccatatt gcaccgcgat
     1381 atcctcgatg tggactttgc cgaactggga aacgagtttg gcgtctctcg ttggccgatc
     1441 atcgggaatc ttccctatgt aatcagtagt cgcgtcgcga tgaacttggt ggcgcaagcg
     1501 cggagtatcg cccgcgccac gattatggtt caacgagagg tcggcgagcg gtatctcgcg
     1561 ccccccggat cacgtgatta cggcttactc agcgtgctcc tgcaggcaac cgggtcgcta
     1621 tcccgtgggt ttcgcgtcgg acctggtgca ttccggccgc aacctcgtat cgaatcgatt
     1681 gtgttgacgt ggatcagcga tccgcctcct gatatggcga ttcaaccact gatcactacc
     1741 gtgaaagcgg cgtttgcaca acgtcgaaaa cagcttcgta atgcgcttcg cggtattccg
     1801 ggtgcatcgg acgatgtcat cgcgacggcg tgcgcacaag tgggaattga tctctcggca
     1861 cgggctgaaa
//
LOCUS       DSCC01000189            1503 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1056603, whole genome shotgun sequence.
ACCESSION   DSCC01000189 DSCC01000000
VERSION     DSCC01000189.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1056603"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..543)
                     /locus_tag="ENN56_02375"
     CDS             complement(<1..543)
                     /locus_tag="ENN56_02375"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HDS74362.1"
                     /translation="MQKPTQAIILAAGKGTRLGSITQNTPKALVRVGDKTLLDHVRTG
                     LAAAGVKHTVIVVSHLAEQIEAHLRKYPISGQTAVTVWQPVPQGTGQACKLAVSKLET
                     GPTWITYADILVDRAEYERMAQNFVDEPCDLMLAVFDVDDPYQGAAVYVDETGKVTEI
                     IEKPERGTSTTRWNSAGIYLS"
     gene            740..>1503
                     /locus_tag="ENN56_02380"
     CDS             740..>1503
                     /locus_tag="ENN56_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74363.1"
                     /translation="MARISLSVYITAQTVNERGSDPAALRRMIDDITAVGAKKVFIET
                     WRGGGIVEEGTLRSVKAAFEAEGFETATGIMPVRGRDPLLEGTDDWDSHKTLGGNSRW
                     GADICYSRPWSREEIQRALKRGAAVFDEYIIDDALCTQCTCTYCREKRGDKSWVDYRQ
                     DLLVDVSREDIVGAARSVNPNIHMILKFPQWYDRLHCYGYDTARQPALFDATWIGTET
                     RDPDTGDFGYTPQYEGFFNVRWHKDAEPNLQGAWFD"
BASE COUNT          324 a          370 c          455 g          354 t
ORIGIN      
        1 ggagagataa atcccggccg agttccaccg tgtagtggac gtgccacgtt ccggtttttc
       61 gatgatttcc gtgactttgc cggtttcgtc gacatagacc gccgcacctt gatacgggtc
      121 gtcgacatcg aatacggcaa gcatgagatc gcacggctcg tcgacaaaat tttgagccat
      181 ccgttcatat tccgcacggt cgacaaggat gtccgcatag gtgatccagg ttggtccggt
      241 ttcgagtttc gaaacggcga gcttacatgc ttgaccagtc ccttgcggga ctggctgcca
      301 gactgtgacc gcagtttggc cggaaatcgg gtatttgcgg agatgggctt cgatctgttc
      361 cgccaagtga cttactacga tgacggtgtg ttttacgccg gcggctgcaa gtcctgtgcg
      421 aacatggtcg agaagtgttt tgtcaccgac tcgaacgagt gcttttggtg tgttctgggt
      481 gatcgatccg agacgggtgc cttttcccgc tgcaaggatg atcgcctgcg ttggtttctg
      541 catagttgtc tcggctggaa ggatgaagac gcaaaacgta ggacattcaa tagtgtacgt
      601 tactgatcga cggacggcaa ccgataccgc gcacacgagg ggtttgctcc acggctccgt
      661 cggcgcgaat gtacctgtca cgtttgactc ggttcagtgt ttggcccgca catcatcgct
      721 cggagagaag ggatagtaca tggctcgaat ctcgctatcg gtctatatca ccgcgcagac
      781 ggtaaacgaa cgtggcagcg atcccgcggc gcttcgccgt atgatcgacg atattaccgc
      841 ggttggcgcg aagaaggtgt ttatcgaaac atggcgcggt ggtggcatcg ttgaagaggg
      901 tacgcttcgt tctgtaaaag ccgcttttga agccgaaggt tttgaaacgg cgacgggtat
      961 tatgcctgtc cgtgggcgcg atccgctgct cgaaggaacg gacgattggg atagtcacaa
     1021 gacgctcggt gggaacagtc ggtggggagc ggatatctgc tacagccgcc cgtggagccg
     1081 tgaggaaatc caacgcgcac tgaagcgtgg tgccgccgta ttcgatgaat acatcattga
     1141 cgacgcgttg tgtacgcagt gtacgtgcac ctattgccgg gaaaaacgcg gagacaaatc
     1201 gtgggtagac tatcgacagg atttgctcgt agacgtgagc cgtgaggata tcgtcggggc
     1261 ggcacgctcg gtaaatccga acatccatat gatcctcaag tttccgcagt ggtatgatcg
     1321 acttcactgt tacgggtacg ataccgcgcg acagcctgca ctcttcgatg cgacttggat
     1381 tggaacggag acacgtgatc cggacacggg tgacttcggt tatacaccac aatacgaggg
     1441 ctttttcaac gttcgttggc acaaagatgc tgaaccgaac cttcaaggtg catggttcga
     1501 cta
//
LOCUS       DSCC01000190            1848 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042968, whole genome shotgun sequence.
ACCESSION   DSCC01000190 DSCC01000000
VERSION     DSCC01000190.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042968"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..795
                     /locus_tag="ENN56_02385"
     CDS             <1..795
                     /locus_tag="ENN56_02385"
                     /inference="COORDINATES: protein motif:HMM:PF14100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74364.1"
                     /translation="PLMGPFGHSVTRGWPSNARPGETADHPHHKSVWIAHGDVNGVDN
                     WSEGKDCGRQIQKDMLRCASGAVCGVLETRNDWVGNDGVKVLEDTRKFTFYAMPGALR
                     MFDVEVEFTATEGPVKFGDTKEGGILAVRVATPIDVPRTGRIRAAEGGVDESETWGKR
                     SAWCDYSGLDEGGNLVGIAILNHPNSNRYPTHWHVRNYGLMTANPYGLHDFAGDETVD
                     GSQTIGKGEKLWFGYRVYVHAGDADGAHVADAFHAYINPPKVTVEV"
     gene            1065..1544
                     /gene="greA"
                     /locus_tag="ENN56_02390"
     CDS             1065..1544
                     /gene="greA"
                     /locus_tag="ENN56_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDS74365.1"
                     /translation="MASIYMTRTGRDKLEDELRMLKTKERPKSRAKLAEAREKGDLSE
                     NAEYDAAKEEHNRLETRISRIQDQLSRAMIIEDGNFPEGQVAIGRTVELEDLGSQRKY
                     TYTLVSEVESDFAAGKISTNSPVGKGLVGHFEGEDVEITVPAGVKRYRILKVRSAGE"
     gene            complement(1572..1823)
                     /locus_tag="ENN56_02395"
     CDS             complement(1572..1823)
                     /locus_tag="ENN56_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74366.1"
                     /translation="MRVRAALITTLSANQPCVGTLDDCADGVTAAECPFFSRVLEDPI
                     QSEEWRKVLGTYRGPAGGYYRYVPEECQIRQVPEPVDTR"
BASE COUNT          389 a          532 c          581 g          346 t
ORIGIN      
        1 ccgctcatgg gaccgttcgg gcactcggtc acccgcggtt ggccgtcgaa tgcgcgtccg
       61 ggcgagaccg cggaccatcc gcaccacaag tcggtttgga tcgcgcacgg agacgtaaac
      121 ggtgtcgaca actggtccga aggtaaggac tgtggtcgtc agattcagaa ggacatgctc
      181 cggtgcgcga gtggagccgt gtgcggcgtc ctcgagactc gtaacgattg ggtcggcaac
      241 gatggcgtaa aagttctgga agacacgcgg aagttcacgt tctacgccat gcccggcgcg
      301 cttcgcatgt tcgacgtgga agtggagttc acggcgacgg aaggtcccgt gaagttcggc
      361 gacacgaaag aaggcgggat tctcgccgtt cgcgtggcga caccgatcga cgtgccgcgt
      421 accggccgca tccgcgcggc ggaaggcggc gtcgacgaat cggagacgtg gggcaagcgc
      481 tcggcgtggt gcgactatag cggcctcgat gaaggcggta acctggttgg aatcgcgatc
      541 ctcaatcatc cgaacagcaa tcggtacccg acccattggc atgtgcggaa ctacggtctc
      601 atgacggcga atccgtatgg cctccatgat ttcgccggcg acgagactgt cgacggttcg
      661 cagacaattg ggaagggcga gaagctctgg ttcggatacc gtgtgtacgt tcatgcaggt
      721 gacgccgatg gcgcgcacgt cgccgacgcg ttccacgcgt acatcaatcc gccgaaggta
      781 accgtcgaag tctgacaaca cgttcgacat tccacgagag ccccgtcgtc gttcccggcc
      841 acggggcttt cgcataccat tcacgattgc tcttggcaac ggagcggtat cgtgttgtat
      901 ctttagagcg tagaggtccg gacgtttttc gttcggacct cgattcctac cgatgcggaa
      961 cgtgttcgga cggcggtgat taccgctgat ccgttccttt ttttgtaccc gtcggtattg
     1021 aatcgcaagc atttcaccgg cactgtgccg aggagcaccg aggtatggca agcatctaca
     1081 tgacccgcac gggacgcgac aagctcgaag acgagctccg catgttgaag acgaaagaac
     1141 gcccgaaatc gagggcgaag ctcgctgagg cgcgtgaaaa gggtgacctt tcggaaaacg
     1201 ccgagtacga tgccgcaaag gaagagcaca atcgccttga gacgcgcatc agtcggatac
     1261 aggatcagtt atcccgcgca atgatcatcg aggacggcaa tttcccggaa ggccaagtcg
     1321 cgatcggtcg aactgtcgaa ctcgaagacc tcggcagcca acggaaatac acgtacacgc
     1381 ttgtatccga agtggaatcg gatttcgcgg cggggaagat ttcaacgaat tcaccggtcg
     1441 ggaagggact cgtggggcac ttcgaaggcg aggacgtcga gataactgtt cccgccggcg
     1501 tcaaacggta ccggatcctc aaggtgcgct ccgcaggtga atgacgtagc gcacaccgcc
     1561 gagccgcggt cctaccgcgt atcgaccggc tcgggcacct gtcgaatctg acactcttcc
     1621 ggcacatacc ggtagtaccc accggccgga ccgcggtacg tcccgagaac tttccgccac
     1681 tcctctgatt gtatgggatc ctccaacacg cggctgaaaa acgggcattc ggccgcggtg
     1741 acgccatccg cgcaatcgtc gagtgtgccg acgcacggct gattggcgct gagcgtggtg
     1801 atcagcgccg cacgtacgcg catgatcgtg gtttgctcgt cctcgacg
//
LOCUS       DSCC01000191            2630 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026597, whole genome shotgun sequence.
ACCESSION   DSCC01000191 DSCC01000000
VERSION     DSCC01000191.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026597"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..937)
                     /locus_tag="ENN56_02400"
     CDS             complement(<1..937)
                     /locus_tag="ENN56_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74367.1"
                     /translation="MKRLNNLVTELYAADKLPGETVSLVHPRTGWLYVSCAEGGAASL
                     NGKPIPFHPHNSRLEAMLYVPAGDVSLEGASARDVVVRAVPELAYCKYGYNPFIREYG
                     TYDWAYLEQHVLPHANTVIGRGVDDERDHAREWKTRGGRWIVERSLPTSRDTEHDPDV
                     VFEKWIDTPGLTDLLFDGILLDEFFSGDKPSFPAFTEAITRIAEDPRFRDKQVYPYCG
                     SHYPDRDDWGTFDENDTSKSSVPFYRATFDAGYRVAWERYMQERHEEAIAEAFIGERM
                     IANMHRWRNVLPDAAAHMIVALGYMDMGFQLDIHPD"
BASE COUNT          532 a          766 c          753 g          579 t
ORIGIN      
        1 cgtcgggatg aatatcgagt tggaagccca tatccatata ccccagcgcg acgatcatgt
       61 gcgcggccgc gtcgggaagc acgttccgcc aacgatgcat gttcgcaatc atccgctcgc
      121 cgatgaacgc ctccgcgatc gcttcttcgt ggcgctcctg catgtatcgc tcccaagcga
      181 cacgataacc cgcatcgaac gtcgcgcggt aaaacggcac gctggacttc gacgtgtcgt
      241 tctcgtcgaa cgtaccccaa tcgtcgcgat cgggatagtg tgagccgcaa tacggatata
      301 cctgtttatc ccggaaacgc ggatcttcag caatgcgcgt gattgcttca gtgaacgcgg
      361 gaaaacttgg tttgtcgccg ctgaaaaact cgtcgagcaa gataccgtcg aagagcagat
      421 cggtgagtcc gggtgtgtcg atccatttct cgaatacgac gtccggatcg tgctccgtat
      481 ctcggctcgt cggcaaactc ctttcgacga tccatctgcc tccgcgcgtt ttccattcac
      541 gcgcatggtc ccgctcgtcg tccacgcctc ggccaatgac ggtatttgca tgcggaagaa
      601 cgtgttgctc gagatacgcc cagtcgtacg tgccatactc gcgaatgaac ggattgtagc
      661 cgtacttgca gtatgcgagc tcaggaaccg cccggacaac gacatcccgc gctgatgctc
      721 cttcgagcga tacgtcaccc gccggaacat acagcatcgc ttccagtcga ctgttatgcg
      781 gatggaacgg aatcggtttc ccgttgagtg atgccgcgcc accctcggcg cacgatacat
      841 acagccaacc agtgcgcgga tgtacgagcg aaacggtttc gccggggagt ttgtcggccg
      901 cgtagagctc ggtgacgaga ttattgagtc gtttcattga atcgtgtcct tctcgatgag
      961 tgcgcgtgcc gtcggatcag ctttcgtcgc caatccagaa tggttgaacc tcgacgaagt
     1021 tgtagagtat ctcttggccg acgggtgcct gtggcgcggc gtctgtgagc caatcggaga
     1081 tcgtgagcgt gatgttcggt ccggtcgcct tgaagacaac aaggtgccag ttgaaatagt
     1141 agtgtgtatc gccgacaaag ggctcgaggg tatgggcgta attgttcggt acgacggcgg
     1201 taaatgacct atcgggaagg atctcagcat cgccgcgaag gaggatcctt acaccatgac
     1261 ggctgttctc ggacctaccc tcgacaaacg ccctgtaatc cccggtgatg agtttcgcgc
     1321 tgtaataccg acccggcaca atgtttctca acggttgtga gagcgtgttc gccgagttcg
     1381 cgcgacgttt catcacgagg gcagtgtcgc cgattgtggt gcgcggccac cgcccttgga
     1441 gatggctgta tcccgggatg ctgacggcgc gcatcgaccg ttcctgctcg acgattggga
     1501 aacgccacgt atcccaccaa tcctcgaagt cggcattccg aatgatatcc ggattgtatc
     1561 gccaaccgaa gcgctccgtg tagagattgg tattcccctc gatgcagtag tgccggtaga
     1621 gtcgcggtgc gaaacgtgcc gactcctcgt cggcataact cgagaggtat tcatatactc
     1681 ccgccaggcc gtcgaactcc tcgtgcgttg cgagatactg tagctgcatg tccatgaaga
     1741 ccttgaaatc gacggatgga aaaagattca ccgactcggt gatagtaaga tatccgagcg
     1801 cgatcatcat ccggcgttgg gcttcggggt aaaacgtgcg ccattctcgt atcgaatcga
     1861 gaaaccgatc gtccatcgca cccaccgcat catcgacgtg gcggaattcg cggaggtacc
     1921 gctcccacga tacccaccag tttgcgcttc cacgttcgcc gtcggctgtt gcggcatcga
     1981 aggtctcgcg gatgaagtgt ccgctgttat ccgatgcttc gggtgcgccg acacgccaat
     2041 cgtcggtaag cggatagtga ccaccgaaat acggatacag cgccacaccg tcgaaccgtc
     2101 ggtcgttcgc gatacgtcgt gccgcttcag cgtatccggc gaacgcgggc gcattgccgc
     2161 cggagaactc gtcgatgagc acaccatctg gttttgtggg atcgtttagc gcgatgtccg
     2221 ccatccacga tacgatagcg tcgggatcgg cgacgttgct gagcccgacc ggcataccgc
     2281 gttcgacgat ccaccgaccg ccacgacatt tccattccgt tgcgcactca acctgatcgg
     2341 cgccgccgga agcaaccatg ctgttgatgt gcggattcac ccagcgttcg aggaacgtcc
     2401 aatcgtagtc gccgaactcg cgtatgtggg gattcgcccc caacttgcag tgcatgagct
     2461 gcggcatcgt tcgtatcgtg agattgtcga aatttccttt ttgcgcgctg acgctgtgcg
     2521 tacccttcgc gagaaagcgc atgacttcgc gacgtccggc gtagtcacgc gatggtatcg
     2581 gcgttccgtc aagcgtaacg gcgtcggtac cgttccatgc gatgtatacc
//
LOCUS       DSCC01000192            1850 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042889, whole genome shotgun sequence.
ACCESSION   DSCC01000192 DSCC01000000
VERSION     DSCC01000192.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042889"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1008
                     /locus_tag="ENN56_02405"
     CDS             <1..1008
                     /locus_tag="ENN56_02405"
                     /inference="COORDINATES: protein motif:HMM:PF00015.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74368.1"
                     /translation="DLATHVLATVSVSLAIFVVCAFGVWIVVSVVRKRCEATIDTLFL
                     DLGNARQSLCDIAANIESVAASVAQSSTESTDRTLDHTTQITNIETAIRTLATELSQI
                     AAHVEDLYITEHDTTDSIQESEDAVASLLDGLRRIRDSARLTTDRMTSFDEHLGKALD
                     SAHVIETVMSKINLLALNAAIEAARAGEDGRRFGIVAEEVRNLADRAMSTADDIVTRT
                     QKTRAGVAEVLEHAQSVVGEASDGTVRANKARTSIDGIADRSKRSSELAQALAASSQK
                     QQTDATTIAEQLNTVTRDAAETADVSHQSIACGENLRNLSERIREICRDDEDDTQQPT
                     D"
     gene            1070..>1850
                     /locus_tag="ENN56_02410"
     CDS             1070..>1850
                     /locus_tag="ENN56_02410"
                     /inference="COORDINATES: protein motif:HMM:PF10672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HDS74369.1"
                     /translation="MPDEMKQIIRVNASAQRRLESGHQWVFSNEVIDKLPEYDSGQLV
                     TIANSNGRAIAEGYVNPHTLIAVRALAGPNQSIDSRFFRDRIVGADAWRRTLYPDLDT
                     YRVFHGEADGLTGLVVDRYADHLTVQVLTAGMENLQPIWLPILEEHFKPRAIIARNDT
                     AFRQYENLSEAVTVLVGSPSDTVEVQEFGVKYAVDIRSGQKTGLFLDRKDNRRALAAF
                     AKDASVLDTFCYVGGWGLSAALAGAASVVGIDSSESALASAR"
BASE COUNT          447 a          528 c          511 g          364 t
ORIGIN      
        1 gatcttgcca ctcatgttct cgccaccgtg tccgtgtccc tcgcgatatt cgtagtctgc
       61 gcgttcggag tctggatcgt ggtgtcggtg gtgcgtaaac gatgtgaagc gacaatcgac
      121 actcttttcc tcgacctcgg aaacgcacgt cagagtcttt gcgacatcgc cgcgaacatc
      181 gaatcggtgg cagcgtccgt agctcagtcg tccacggaat ccaccgatcg gacgctcgat
      241 cacacaacac agatcacgaa tatcgaaacg gcaatccgaa ctctggcgac cgaattatcg
      301 caaattgcag cgcacgtaga ggatctgtac atcactgaac acgatacgac ggattccatt
      361 caggaaagtg aggacgcggt cgcgagcctc ttggacggat tacgccggat acgtgactcg
      421 gcccgactga ccaccgaccg gatgacttca ttcgatgagc atttggggaa agccctcgat
      481 tccgcccacg tgatcgaaac cgtaatgtcc aaaatcaact tactcgcgct caatgctgct
      541 attgaagccg cacgcgcggg cgaagatggg cggagatttg gtatcgtcgc ggaggaagtt
      601 cgtaacctcg ctgatcgtgc gatgtcaacc gcagacgata tcgtaacacg cacgcagaag
      661 acgcgtgctg gcgtcgccga agtgctggag cacgcacaga gcgtcgtagg agaagcgtcc
      721 gatggaacgg tgcgcgcgaa caaagcacga acgtcaatcg atggaatcgc agaccgctca
      781 aaacggtctt cagaactcgc acaagccctt gcagcttcct cgcagaaaca gcaaactgac
      841 gccacgacca tcgctgaaca acttaacacc gtcacgcgcg acgcagcaga aacggctgat
      901 gtttcccatc aatcgatagc atgtggagaa aatctgcgaa acctttcgga acgcatccgt
      961 gaaatatgcc gcgatgacga ggatgacacg cagcagccca ccgattagcc gcgcatcact
     1021 tcgtcggcgt gtgtaacgcg tcaattccct gcctacagaa agcgatagaa tgcctgatga
     1081 aatgaaacag atcatacgcg tgaacgcgag cgcacaacgg cgtcttgaga gcggccacca
     1141 gtgggtattc agcaacgagg tcatcgataa gcttccggaa tacgattccg gacaactcgt
     1201 gacgatcgcg aatagcaacg gacgagcaat agcggaggga tacgtaaatc ctcacacgct
     1261 gatcgccgtt cgcgcgcttg ccggaccgaa tcaatcgatc gacagccgtt tttttcgcga
     1321 ccggatcgtt ggagccgatg catggcggcg tacgctgtat ccggatctcg acacgtaccg
     1381 cgtctttcac ggagaagccg acggcctgac cggtctcgtc gtcgaccggt atgccgacca
     1441 cctcaccgtt caagtgctca ccgcgggcat ggaaaacctg caaccgatat ggctaccgat
     1501 actcgaagag cacttcaagc cgcgcgcgat tattgctcgc aacgatacgg cgtttcgcca
     1561 atatgaaaac ctctcggaag cggtaacggt cctcgtcggt tctccttccg atacggttga
     1621 ggttcaagag tttggtgtga aatacgccgt cgacattcga agcgggcaga aaactggatt
     1681 gtttcttgat cggaaggaca accgtcgagc gctcgcagcc ttcgcgaaag acgcgagcgt
     1741 actcgatacg ttctgttacg tcggaggatg gggactttcg gctgcactcg ccggagcggc
     1801 atccgttgtc ggaatcgatt cgtccgagtc ggcgcttgcc tccgcgcgta
//
LOCUS       DSCC01000193            4219 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1014124, whole genome shotgun sequence.
ACCESSION   DSCC01000193 DSCC01000000
VERSION     DSCC01000193.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1014124"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(21..1382)
                     /locus_tag="ENN56_02415"
     CDS             complement(21..1382)
                     /locus_tag="ENN56_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74370.1"
                     /translation="MRLRYVAITVALGIVVLGMEKPITSRSETATKANEWGTAPSDSL
                     ITVVFRAQSTQVVRTDSGFVKRYIGDVVMRMNPDVWVTANQAVYDGIRREARLIGDVV
                     AVDSGRTLRADRIDYLFEDHREAHGRPLARLRQNVSIVDSTRRISTYAADYWTEDDSV
                     VAFGIVRVSLPGGTLDSDRLVYSAKIGDMSASGKVKLYDSTQGITIRSEQYQWFQRDS
                     VAFVSGRPLLTKGEGDTMVFVEADFMRYDQRSNQAVAWDSVRIDRGGLSAYCDSVVYD
                     SGQEQLTLYGSPQAIQRTSGDTATTISETTGERIVLQLDGSDVREIRIFGTRAHPARA
                     IATEFDRSRTKAGERWITGDEVVFHTRDDRVERLEIFGQARSRNAPAPSARTSEGINE
                     ASGDTMVITFEDNRIRKVFLRGGVEGLFRPPVDSSAVDTTVTSEILAPNEQGDGTMVD
                     DGN"
     gene            complement(1386..2123)
                     /locus_tag="ENN56_02420"
     CDS             complement(1386..2123)
                     /locus_tag="ENN56_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74371.1"
                     /translation="MTRRGYLATTIYALLIGLLVACGDLGQEPDPPFDVGEASQTIAG
                     TRIVWSKSGRTSAVIRANVLRRYHDRSEIQLEDSVEVDLYDDDGRLAAIVQGDAGIIR
                     ENVRTAEVDSGITVRFLGTDDYFASIMTAQTAHADDRLKQVTARGDVQVVSESGVSLS
                     TNLLIWEGRRERFRAPGRVTITNGNDIEAGENLVANADLTEWTMQDISGRTTRSSDQI
                     GSLRNTTAPKIDSGTQSRDTGDTRFGF"
     gene            complement(2125..3042)
                     /locus_tag="ENN56_02425"
     CDS             complement(2125..3042)
                     /locus_tag="ENN56_02425"
                     /inference="COORDINATES: protein motif:HMM:PF03279.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74372.1"
                     /translation="MPNVSFRTRARRRVRRDITLGLATLLNRLPRRTAVRLGRVLGRT
                     LAVTCRSERQKAVVRVADALELSHEAASELVERCASAFGGHLADALRLENMSSDELRD
                     IVRLDGLEHLSQALERGHGAILLSAHIGNWEMLAAALSDAGVPLSVIGRKPSDPVYAE
                     RLERIRRRWGVATLWREHGARPIFRALENGRAVGALIDQATRVGSVRVPFFNRPAWTP
                     VGPARIAVRTRAPVIPVHMVRRHDDSNRYIGVIEPEIVSNGDTPEVITGIWTARIEEW
                     IRDATDSWVWMHYRWQDVPSSLRSNTEAR"
     gene            complement(3058..>4219)
                     /locus_tag="ENN56_02430"
     CDS             complement(3058..>4219)
                     /locus_tag="ENN56_02430"
                     /inference="COORDINATES: protein motif:HMM:PF00117.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74373.1"
                     /translation="KIETYGNIDIDHIISAPDVDTVYKIPLNFEAEGIGDKMLDHFGL
                     PARRTPDWSDWIRRVSRITNPDRTLKIAMVGKYVEIGDFALTDSYVSVNQALEHAGAE
                     IGANVEIKWVTATEFENDKESIESLREFDGLLVPGAFGATGAEGMISSIRFARENKLP
                     YLGLCYGLQMAVIEYARNVAGMTDANSTEIDKATPYPVITVMDEQRTIIDPEVWERWR
                     EISPQIELVANAWEGGTHQLGGTMRLGSYAARLREGTLIHQLYQEVNRLDADRQQLEK
                     MRKERNQGFRIGRFDESGPVVIERHRHRYEVSPDYVQQLEDAGLVFSGFHETADGTRL
                     MEFIELPTNVHPYFVATQAHPEFRSRLEAPAPLFLGFLRAALEHSVSQDSKK"
BASE COUNT          884 a         1314 c         1045 g          976 t
ORIGIN      
        1 cggcaccatc cggttcgttg tcagttgcca tcatcaacca tcgttccgtc gccctgttcg
       61 ttcggcgcca gtatctcgga agtcaccgtc gtatctaccg ccgaggaatc aacgggtggt
      121 ctgaataacc cctcaacgcc gccgcgtaaa aaaaccttac gaatccggtt gtcttcgaac
      181 gtaatcacca tggtatcacc cgaagcctca ttgattcctt cggacgttcg ggcagatggc
      241 gccggtgcgt ttcggcttcg cgcctggcca aagatctcca gccgctctac gcgatcatcg
      301 cgggtatgga acacaacctc gtcgccggta atccatcgtt ctccggcttt cgtacgcgat
      361 cggtcaaact ccgttgcgat cgctcgagca ggatgcgcgc gcgttccaaa tattcgtatc
      421 tcgcgcacgt cggagccatc caactggaga acaattcgtt caccagtcgt ttcgctgatc
      481 gttgtcgcag tgtctccact ggtacgttgg attgcttgcg gagagccgta caacgtgagc
      541 tgttcttgcc cgctatcata gacaaccgaa tcgcagtacg cggaaagtcc accacggtcg
      601 atgcgaacgc tgtcccacgc gaccgcttga ttggaccgct gatcgtatcg catgaagtcc
      661 gcctcgacaa ataccatcgt gtcgccttca ccttttgtca ggagcggacg tccactcaca
      721 aatgccacgc tgtcccgttg aaaccattgg tattgttccg atcgtatcgt tatcccctgc
      781 gtgctatcgt ataatttcac tttgccggaa gccgacatat ctccgatttt cgccgaatac
      841 accaaccggt ccgaatcgag cgtaccacct ggtaacgata cccgaacaat accgaacgca
      901 accacactgt cgtcttccgt ccaatagtcc gcagcgtagg tactgatccg acgcgtgcta
      961 tcgacaatcg acacgttttg acgaagccgc gcgagaggcc gaccatgcgc ctcgcgatgg
     1021 tcttcgaaga gataatcaat ccgatctgca cgcagagttc ggccggaatc gaccgcaacg
     1081 acgtcaccaa tgagcctcgc ttcgcgtcgg attccgtcat acactgcctg attcgctgtc
     1141 acccacacgt ccggattcat tcgcatgacg acgtcgccaa tgtatctctt cacgaatccg
     1201 gagtcggttc gtacaacttg cgtgctctgt gctcgaaaca ccaccgtaat aagcgaatcg
     1261 ctcggcgcgg taccccattc attcgctttc gtagctgttt cgcttcggga tgtaatcggt
     1321 ttttccattc ccaaaacgac gataccgagg gcaaccgtaa tcgcaacata gcgaagtcgc
     1381 atcggtcaga atccaaaccg agtgtcgccg gtgtcacgag attgcgtgcc tgagtcgatt
     1441 tttggcgcgg tcgtgttacg caaactaccg atttggtcgg acgaacgcgt tgttctgccg
     1501 ctaatgtctt gcatcgtcca ttccgtcaaa tccgcgttcg cgacgagatt ctcacccgcc
     1561 tcgatatcgt taccgtttgt aatcgtcacg cggccaggtg cgcgaaagcg ctcgcgccga
     1621 ccctcccata tcaaaaggtt tgtcgagagc gatacgccac tttcgcttac aacctgcaca
     1681 tcgccgcgcg cagtaacctg cttcaatctg tcgtctgcat gtgccgtttg tgccgtcata
     1741 atcgacgcga agtaatcgtc cgttccgaga aacctcactg ttatgccgct atcaacctct
     1801 gccgttcgca cattttcgcg gatgattccc gcatctcctt gcacgattgc ggcgagccta
     1861 ccgtcatcat cgtacaaatc aacttcgacc gagtcttcga gttgtatctc gcttcgatcg
     1921 tgataccgac gcagtacgtt tgcgcgaatt accgcgctcg tccgtccgct cttcgaccaa
     1981 acaattcgcg taccagcgat tgtttgcgat gcttcgccga catcaaacgg aggatcgggc
     2041 tcctgcccta aatcgccgca cgccacgagg aggccgatca aaagggcata tatcgtcgtc
     2101 gcgagatatc cacggcgtgt cacgttatcg cgcctctgta ttcgaacgca aagaactggg
     2161 aacgtcctgc caccggtaat gcatccacac ccatgagtcc gtggcgtcac gaatccactc
     2221 ttcgatccgc gccgtccaaa taccggtgat gacttccggc gtgtcgccgt ttgaaacgat
     2281 ctccggttcg atcacgccga tatatcgatt cgaatcatcg tgtcgacgca ccatatgcac
     2341 cggaataacc ggagcccgcg tgcgcaccgc gattcgcgcc ggtcctaccg gtgtccacgc
     2401 cggccgattg aagaacggga ctcggacgct tccgactcgt gtagcttgat caattaacgc
     2461 tccaaccgcc cggccgttct caagtgcccg aaaaatcggt cgcgcaccgt gctcacgcca
     2521 cagcgtcgct acgccccaac gccgccgaat tcgttcgagt cgttcggcgt aaaccgggtc
     2581 ggacggtttc cgtccgatca cactaagcgg cacgccggca tcggaaagcg ccgcagcgag
     2641 catttcccag tttccgatat gtgcactcag gagtattgcg ccgtgaccgc gctccaatgc
     2701 ctgcgaaaga tgctcgagcc catcaagtcg cacgatatca cgcaactcat ccgagctcat
     2761 gttttcgagt cgaagcgcat ctgccagatg accaccaaac gccgatgcac atcgttcgac
     2821 gagctcgctt gccgcctcat gagacaactc aagcgcatcg gctacacgga ctaccgcttt
     2881 ttgtcgttcg gaccggcacg tgaccgcgag tgtgcgcccc aacacgcggc caagccgtac
     2941 cgccgtgcgt cgcggcagtc gattgagcag tgtggcgagt ccaagcgtaa tgtcccgtcg
     3001 cacgcgacgt cttgcgcgtg tgcgaaacga aacattcggc atcttgcctc cgccgtctta
     3061 tttcttcgaa tcctgcgaaa cgctgtgttc aagtgcggcg cgcaggaatc cgaggaacaa
     3121 cggcgccggt gcttcaagac gcgagcgaaa ctcgggatgc gcctgtgttg cgacgaaata
     3181 cggatgaaca ttcgttggta gctcgataaa ctccatgagt cgcgttccgt cagctgtttc
     3241 atggaatccg ctaaagacga gccccgcatc ctcgagctgt tgcacgtagt cgggcgaaac
     3301 ctcgtaccgg tgccgatgcc gttcaatgac gaccggaccg gattcgtcaa accggccgat
     3361 tcggaatccc tgattccgtt cttttcgcat cttctccagt tgttgccgat ccgcgtcgag
     3421 acgatttact tcctggtaca actgatgaat cagggtccct tctcgaagtc gcgcagcata
     3481 tgacccgagt ctcatggttc cgccaagttg atgtgtgcca ccttcccacg cgttcgccac
     3541 aagttcgatc tgcggactga tctcgcgcca acgttcccac acctcgggat cgataatcgt
     3601 tcgctgctcg tccatcaccg tgatgaccgg atacggtgtc gctttgtcga tctccgttga
     3661 attggcgtcg gtcataccgg cgacgttccg cgcgtattcg atcaccgcca tctgaagacc
     3721 gtaacataat cccaaatagg gtaatttgtt ttcccgagcg aaacgaatcg acgaaatcat
     3781 tccttcggcg cctgtggctc caaacgcacc cggcacgagt aacccgtcga actcgcgtag
     3841 agattctata ctctccttgt cattctcgaa ttcggtggcg gtaacccatt tgatttcaac
     3901 gtttgcaccg atttccgcac cggcgtgttc aagtgcttgg ttaaccgata cgtaactgtc
     3961 tgtgagtgcg aaatcaccga tctcaacgta tttgccaacc attgcaatct tcaacgtgcg
     4021 gtcgggattc gtaatccggc tcacccgccg aatccaatcg ctccaatcgg gcgtgcgtcg
     4081 agcaggcagc ccgaaatggt cgagcatctt atcgccgatc ccctccgcct cgaaattgag
     4141 cgggatcttg tacaccgtgt cgacatccgg ggcgctgatg atatggtcaa tatcgatatt
     4201 accatacgtt tcaattttc
//
LOCUS       DSCC01000194            1807 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044246, whole genome shotgun sequence.
ACCESSION   DSCC01000194 DSCC01000000
VERSION     DSCC01000194.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044246"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            322..>1807
                     /locus_tag="ENN56_02435"
     CDS             322..>1807
                     /locus_tag="ENN56_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDS74374.1"
                     /translation="MNSLVLAGIAFAGYIVAYRFYGRFLARRIFAIDPNAVTPAHALR
                     DDRDYVPTRRQIVFGHHFTSIAGTGPIVGPAIGIIWGWLPAFIWVFLGPIFIGAVHDF
                     GAIVLSARNRGRTIGDTAGQVINRRTRVLFLLIIMFLLLIVIAIFAMIIGILFTMYPA
                     SVFPIWAQIPIAVGLGWVIRNTDIPTLPASIVAVILLYVTIGIGAAMPLTLPPLFGFT
                     PLTIWVIVLLIYAYIASVLPVWRLLQPRDYINGHQLYVAMAALILGMIVLHPVMTAPV
                     LDLHPEGAPNLIPGIFIIIACGAISGFHSLVSSGTTSKQISSETDALPIGFGGMLLEG
                     ALAVIVLVAVGAAIGDANTWSAHYSSWQASDGLGAKLGAFVSGGANMLAAIGIPHAFA
                     ATAIGVFIASFAGTTLDSATRLQRYVIGELADAINVKPLASKHGATTVAVVSAAVLAL
                     GQGGGTGGLVLWPLFGASNQLLGGLALLVITVYLIRQRKNSLVTG"
BASE COUNT          325 a          506 c          526 g          450 t
ORIGIN      
        1 aagccgtccc gcagtgagtg tcagcggatt catgttggcg agcttgataa acggcgcggc
       61 ggtgcttacg gcgagggtgc cggtgatcag cacgccgaac gttctgacgc gattcggagc
      121 ggcttcggtc acgatgttcc ttattgaatg tgcacatagc gtgatgatgc aaaggaactc
      181 gaaatatggc tcttgccaca aacccacgca acgcgggcgg ttgacacgca agcgtatcgt
      241 tgtctatttc gtacgcctgt cacttccgct cgcgacgtct gccgccgcgt ggcggttccg
      301 tattcattgg agacctctcg tatgaattcg cttgtacttg cgggcatagc atttgccgga
      361 tacatcgtcg cgtatcgttt ctatggacgt ttccttgcgc ggcgtatatt cgcgatcgat
      421 ccaaacgccg ttacaccggc gcatgcccta cgtgacgacc gcgactatgt cccaacgcgt
      481 cgacagattg ttttcgggca tcatttcacg tcgatcgccg gaacgggacc gattgtaggt
      541 cccgcgattg gcatcatctg gggatggcta ccagcgttca tctgggtatt tctcggaccg
      601 attttcatcg gcgccgtgca tgatttcggg gcgattgttc tatcggcgcg gaaccgtggg
      661 cgaacgatcg gtgacaccgc cggtcaagtc atcaaccggc gtacccgtgt gctctttctc
      721 ctcatcatta tgttcctgct actaattgtt attgcgatct tcgcgatgat catcggtatt
      781 ctcttcacga tgtatcccgc gtcggtgttt ccaatctggg cgcagattcc cattgcagtc
      841 ggacttggtt gggtgattcg caatacggac attcctacgc ttccggcaag tatcgtagcg
      901 gtgatactgc tctacgtgac gattgggatc ggtgcagcga tgccgctgac gttgccaccg
      961 ttattcggat ttacaccact tacgatctgg gtgatcgtcc tgttgatcta cgcgtatatc
     1021 gccagtgtgc ttcccgtctg gcggctcctg caaccgcgtg attacataaa cggtcaccaa
     1081 ctctatgtcg cgatggccgc gctcattctc ggcatgatcg ttctccatcc ggtcatgacc
     1141 gcaccggttc tcgatctaca ccccgaaggt gcaccgaacc tcatcccagg aatctttatt
     1201 atcatcgcgt gcggtgcgat atcggggttt cacagtcttg tgtcgtcggg aaccacgtcg
     1261 aagcagattt cgagcgagac tgacgcgtta ccgattggct tcggtggaat gttattagaa
     1321 ggtgcgctcg cggtcatcgt gctcgtcgcc gtgggagcag caatcggcga cgcgaacacg
     1381 tggagcgcgc actactcgtc gtggcaggcg tccgacggtc tcggcgcaaa gcttggcgcg
     1441 ttcgtttccg gcggcgcgaa tatgttggcg gcgatcggga ttccccacgc gtttgcggcg
     1501 acggccatcg gcgtgtttat cgcttcattt gccggcacga cgctcgatag tgcgacgagg
     1561 cttcaacgct acgtgatcgg cgagttggcc gacgccatca acgtaaaacc gctcgcgtcg
     1621 aagcacggtg cgacaacagt cgcggtggta agtgcggcgg tgctcgcact tgggcaaggt
     1681 ggcggtacgg gcggactcgt gttatggccg ctttttggcg cgagcaatca gcttcttggt
     1741 gggctcgcgc tactcgtcat cacggtctac ctcattcgtc agcgaaagaa cagcctcgtt
     1801 acaggca
//
LOCUS       DSCC01000195            2046 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037336, whole genome shotgun sequence.
ACCESSION   DSCC01000195 DSCC01000000
VERSION     DSCC01000195.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037336"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..438)
                     /locus_tag="ENN56_02440"
     CDS             complement(<1..438)
                     /locus_tag="ENN56_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74375.1"
                     /translation="MTNGDRVGQPATTTIRVGELTFTGASVSAYTTFIYSPELKVIFD
                     MGSVVEEMLPIGHVCISHGHQDHLLGLSRYVGLRRLQHMSPPTVFLPSAIVDRVERLL
                     ECWQDIEGEGRRRAPAADLVGVNDGEEYPLRGNLLVQPFTVHHT"
     gene            complement(435..1553)
                     /locus_tag="ENN56_02445"
     CDS             complement(435..1553)
                     /locus_tag="ENN56_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74376.1"
                     /translation="MKVIAHPLPEAFERKHGTLSDLRSITHDPKFRQDWISTTTVRWN
                     PNDGYVYLGLTQMDGDILYRFDRKTGHAECLDYLSIRVENEVKVHRSLEITPDGRLFG
                     ASADLITIKDRETAPGGQIWSYDPQSKAYDVFGIPSPGDYIQHITVDFNRMIAYGCTY
                     PVPWFFAFDLKSRKTIRKVFIGAYPHKTGIDDKGRVWTGYSATADVGDGENSLMCYDP
                     HTDTVEWTDLQLPAIGRKDDKQMDDIVNLGDGYLYMGSVSGGFSRLDPERREIEWLGK
                     PSRGMRLCGIDEGPDGSIYVTTGANYGMKPDDAVTRAHRFDRHTKQFVDLGPIYDPKF
                     GSGCFALHALSIDDAGTLWVGETDNGERSGCLWECRIT"
     gene            1751..>2046
                     /locus_tag="ENN56_02450"
     CDS             1751..>2046
                     /locus_tag="ENN56_02450"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74377.1"
                     /translation="MTKTTRPNVFLVCADHLRNDALGCNGNPFVHTPNIDRLAASGVT
                     FRNSFSPNPICVPARASVTTGNYPHRATGVTANSGRIRDDQPKLAEHFNNAGYGT"
BASE COUNT          479 a          602 c          520 g          445 t
ORIGIN      
        1 cgtatggtgt acggtgaacg gttgaacaag aaggtttccc cgtaatggat actcctctcc
       61 gtcgttcaca ccgaccagat cagccgccgg tgcccgtcga cgtccctcac cctcgatatc
      121 ctgccaacat tccagaagcc gctccacgcg gtccacaatc gcagacggga gaaataccgt
      181 cggtggagac atatgttgta gtcgtcgcaa accaacgtag cgagatagac cgagtaaatg
      241 gtcttgatgc ccgtgagata tacacacgtg tccgatggga agcatctctt caacgacgct
      301 tcccatgtca aaaatcacct tgagctccgg cgagtagatg aacgtcgtat acgccgaaac
      361 ggatgctccg gtaaacgtca gttcgccgac acggatcgtc gttgtcgcag gctgcccgac
      421 acgatcgcca ttcgtcacgt gatacgacac tcccaaagac atccggaacg ctcgccgttg
      481 tcggtctcac caacccataa cgttcccgca tcatcgatgg acagcgcatg tagcgcgaaa
      541 caccctgagc cgaacttcgg atcgtagatc ggaccaagat caacgaattg cttcgtgtgt
      601 cggtcgaaac ggtgcgcgcg agtcaccgcg tcgtctggct tcattccata gttcgcgccg
      661 gttgtgacgt agatgctccc gtccggtcct tcatcgatgc cgcaaagccg cattccacgc
      721 gacggcttcc cgagccattc gatttcgcgc ctctccggat cgaggcgcga aaacccaccg
      781 cttacactac ccatatagag atacccgtca ccgagattta caatgtcatc catttgcttg
      841 tcgtctttac gaccaatggc gggcagttgt aggtccgtcc attccacggt gtccgtatgc
      901 ggatcgtagc acatgagaga gttttcgcca tcgcccacat cagctgtcgc cgaatagccc
      961 gtccatacgc gacctttgtc gtcgataccg gtcttgtgcg ggtacgcgcc gatgaaaacc
     1021 tttcgaatcg tctttcgtga cttcaaatca aaggcgaaaa accaaggtac cggatacgtg
     1081 catccgtacg cgatcattcg gttaaaatcg acggtgatgt gttggatgta gtcaccgggc
     1141 gacgggatac caaacacatc gtacgccttg ctttgtggat catacgacca aatctgacca
     1201 cccggagccg tttctcgatc ctttatcgta atcaaatcgg cgctggctcc gaataagcgt
     1261 ccgtccggag tgatttcgag cgaccggtga accttcactt cattttccac gcgaatcgac
     1321 aggtagtcga ggcactctgc gtgacccgtt ttacggtcga aacggtacag gatgtcaccg
     1381 tccatctgcg tgagaccgag atagacgtat ccgtcgttgg gattccaacg gacggtggtt
     1441 gtcgatatcc agtcctgacg aaactttgga tcgtgagtga tcgaccgaag atctgaaagc
     1501 gtcccgtgct tgcgttcgaa cgcttctgga agcggatgcg caatgacttt catgtcaatt
     1561 ctcctcaatc gagtgataca gcgtgcatca tcttcgtagc gcccgaagat acgaacgcac
     1621 gtatttgaac gccacgacac gactcgtagt agcatgggtt cacgcgtaga accttgcccc
     1681 gttttcgctc aggacgcatg ttcggaaggc ctcgggtaac gatgttttgt cgtcggaaag
     1741 ggaacgggat atgacgaaaa caacgcgacc gaatgtattc ctcgtatgcg ccgaccatct
     1801 ccgcaatgat gcactcggat gcaacggaaa cccattcgtt catacaccaa acatcgaccg
     1861 gttggcggcg agcggcgtaa cgttccgcaa ctcgttttcg ccaaacccca tctgcgttcc
     1921 cgcacgagcg agcgtaacca ccggcaacta cccacaccgc gcaaccggag ttactgcgaa
     1981 tagcggtcgc atacgcgatg accagccaaa actcgcggaa cacttcaaca acgcggggta
     2041 cggaac
//
LOCUS       DSCC01000196            1876 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042032, whole genome shotgun sequence.
ACCESSION   DSCC01000196 DSCC01000000
VERSION     DSCC01000196.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042032"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(264..1124)
                     /locus_tag="ENN56_02455"
     CDS             complement(264..1124)
                     /locus_tag="ENN56_02455"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02811.17,HMM:PF13263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDS74378.1"
                     /translation="MIDLHIHTNCSDGREFPEAMVQYAIELGLDALGITDHDTIAAIA
                     PAEAGANGTIRIVRGIELSATEGDTPIHLLGYGIDPNHVTLNAVLTDVVADRRNRAER
                     IVAELNDMGLDVTLDMVEDESGDAPITRAHIGYALLNNGYVRNTGEAFARYLSNDAPA
                     FFPQTALDIKTAIATIHDAGGIAVYAHPGITKRDELIARMVREGLDGIETIHPQHTRT
                     TIRFYERLARKHGLIATGGSDAHGRMRMAEIATSTPIPHDMLRVIDEAIAQRRTATCG
                     TLAASTPSVS"
     gene            complement(1287..>1876)
                     /locus_tag="ENN56_02460"
     CDS             complement(1287..>1876)
                     /locus_tag="ENN56_02460"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS74379.1"
                     /translation="GAPIDELSRREVARRIAVVPQETSFTFAFSALEVVLMGRSPYLG
                     RFALEGASDLAVARTAMDSTDTWPLRDRSMDAVSGGEKQRVVISRALAQEPSILLLDE
                     PTTALDIRHQVDIYRLLNRLHQDARHTVVLVQHDLNLAAKVCQRLILLNRGNVCCDGA
                     PADVIRADLLSEVYETDLQVETDPNSGSPVVIVPL"
BASE COUNT          375 a          538 c          576 g          387 t
ORIGIN      
        1 aaggaggcgt aacgctatct caggctttcc ccgtcgcatg gcaaccgcga ccgaatcacc
       61 gacgtgcggc ggctcttgcg cgggagtggg attcgccgcg cagagaatcg tgtacacggc
      121 gaacaccgtt gccgtgtatc ggccatatcg tggggaatgt acggacggtc gcttcacggg
      181 agtgcccagc gaatcgggat gtcccccgcg acacattcgg gacgcctgtc cggggatctc
      241 tgactgcttg tgatcggtag gacttatgat acggacggcg tactcgcggc caaagttccg
      301 cacgtggccg tccgtcgttg tgcaatcgcc tcgtcgataa ctcggagcat atcgtgcgga
      361 atcggtgtcg aagtggcgat ctcggccatg cgcattcggc catgcgcgtc cgaaccaccc
      421 gtcgcgatca atccgtgctt ccgcgcgagc cgttcgtaga atcggatggt tgttcgcgtg
      481 tgttgcggat gaatcgtttc tatcccgtcg agaccctcgc gaaccatgcg cgcaatgagt
      541 tcgtcccgct tggtgatacc cggatgcgcg tatacggcga tgccgcccgc gtcgtgaatt
      601 gtggcgatcg ccgtcttgat gtcgagagcc gtttgcggaa aaaatgccgg cgcgtcgttg
      661 ctcaggtacc gcgcgaatgc ctctccggtg tttcgcacat acccgttatt gaggagcgcg
      721 tagccgatgt gcgcacgcgt gatcggagca tcaccggact catcctcaac catatccagt
      781 gttacatcga gccccatgtc gttgagctcg gcgacgatgc gctcagcgcg gttacggcga
      841 tcggcgacaa catcggtaag caccgcgttt aatgtgacgt ggtttggatc gataccgtac
      901 ccgagaagat gaatcggcgt gtcaccctca gtcgcgctta actcgatgcc tcgaacgata
      961 cggatagtcc cgtttgcccc cgcttcggcc ggtgcgatcg ccgcaatggt atcgtgatca
     1021 gtgataccga gtgcgtccag accaagttcg atcgcatact gcaccatggc ctcaggaaat
     1081 tcccgtccgt cggagcagtt ggtgtgaata tgcagatcga tcattgcgcg ttctcacaaa
     1141 cgggcggtaa acgcaccgct ggaatcgaaa aggtgatgaa cgtacgggag atgatccgtt
     1201 cagtcaatgc ggttcgtctg aaaggaggcg gaagtaactg acagtgttcg gcacgcctta
     1261 cgtgcggtta tgctatcgca gaaggtttac agcggaacga tcacgaccgg tgatccgcta
     1321 ttgggatcgg tctcaacctg gaggtccgtt tcgtagactt cgctcagcaa gtcagcgcga
     1381 atcacgtccg ccggagcgcc gtcgcaacaa acatttccgc gattgaggag gataagtcgt
     1441 tgacagacct tcgctgcgag gttgaggtcg tgctgcacaa gaacgaccgt gtggcgcgcg
     1501 tcttgatgaa gccggttgag gagccgatag atatcgactt gatggcggat gtccagcgcc
     1561 gtcgtcggct cgtccagcaa gaggatcgac ggttcctgag cgagtgcccg cgatatgact
     1621 acgcgctgct tctcgccgcc ggataccgca tccatagagc gatcgcgcag tggccacgtg
     1681 tcagtcgaat ccatggccgt acgcgcgacg gcgaggtcgc tcgcaccctc caacgcgaat
     1741 cgtccgagat agggcgaacg gcccatgaga accacttcga gcgcgctaaa cgcgaaggtg
     1801 aacgatgtct cctgcggcac gacggcaatc cgacgggcga cttcacgccg tgacaattcg
     1861 tcgatcggag caccgt
//
LOCUS       DSCC01000197            1759 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045839, whole genome shotgun sequence.
ACCESSION   DSCC01000197 DSCC01000000
VERSION     DSCC01000197.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045839"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            607..1341
                     /locus_tag="ENN56_02465"
     CDS             607..1341
                     /locus_tag="ENN56_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDS74380.1"
                     /translation="MKGVILAGGTGSRLFPLTKVTNKHLLPVYDKPMIYHPIQALVNA
                     GIRDIMLVTGGESAGDFLRLLGSGSEFGLKHLNYAYQEKPGGIAQALGLARDFIGDDR
                     FVVILGDNIVQKSIKHAVDGFQKQAEGAKIMLAEVENPKAYGVARIENEKIVEIIEKP
                     ATPPSNWAVIGIYLFTPDVFDVIVTLKPSARGELEITDVNNEYIRRGTMSFDYIDGWW
                     ADCGESIDWYLRANNLVAQDGANHVD"
     gene            1399..>1759
                     /locus_tag="ENN56_02470"
     CDS             1399..>1759
                     /locus_tag="ENN56_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HDS74381.1"
                     /translation="MTELIIAMAFAIGVSFICSLLEAVLYSVPLSHIETLAEEERPAG
                     RILRELRDDIDAPISAILTLNTIAHTVGAAVAGAAAAEVFGSKWLGAFSAVFTFLILV
                     LSEIIPKTAGVTYARELA"
BASE COUNT          390 a          436 c          533 g          400 t
ORIGIN      
        1 atatcaagac gacgtaacgc ttcgacgata ggctcgggaa gctgttccgc cggttttgaa
       61 tagcgagccg gacgcgccgg aatatcttgg agatgaacga tctggtcgcg ccagccacgc
      121 gtgttcgtga cctgtgacag aaaccggcga acatccatgg tgcgtcctca atcggtggtt
      181 cgtgcagtat tcgggaatta cgttgtgcgt ctgctgacga ttcgtgcgga attcgcgtcg
      241 gcgtaaaacc ggtcgctcgg gtgccatgac cgctcacagg cgtcgcactg ggcgacatcg
      301 gtggcaatat aaagtgtgac ttgcggtgtt ccacacactg ggcacttcgc tggctgttta
      361 ccgaattttt ccgcagttag tgtcgtgctt ggtaggctgt atgccatgta tcgttgtctc
      421 ttcgttcggc ggtttcatgg gatgccgtat aaagtatatg gagtgacacc cgtgtcggtg
      481 aaatacgaag cgcgggtttt cttcgagaag cgagtacggt tgccgagacg ttgccatacc
      541 gaaggaatcg ttacagattc aagtgcgcac gaaacgtgaa agaccacgaa cgacggaggg
      601 gaatagatga agggcgtgat tcttgcgggg ggcaccggtt cacggctctt tccgctgacg
      661 aaggtgacga ataagcatct gctgcccgtt tacgacaagc cgatgatcta tcacccaatc
      721 caagcgctgg tgaatgcggg cattcgcgat atcatgttgg tgaccggtgg cgaaagcgcg
      781 ggagacttcc ttcgcctgct cggaagcggt agcgagtttg gcctcaagca tctgaactac
      841 gcgtatcagg aaaaaccggg aggaattgca caggcgttgg gactcgcgcg tgacttcatc
      901 ggagatgacc gatttgtggt gattctcggc gataacatcg tgcagaagtc gatcaagcac
      961 gcagttgatg ggttccaaaa gcaagctgaa ggcgcgaaga tcatgctcgc cgaagtggag
     1021 aatccgaagg catacggcgt ggcacgaatc gagaacgaga agatcgtcga gatcattgaa
     1081 aaacctgcga ctccaccgtc aaattgggcg gtgatcggca tctatctgtt tacgccggac
     1141 gtgtttgacg ttatcgtcac gctgaagccg tcagcgcgcg gcgaactcga gatcaccgac
     1201 gtgaacaacg aatatatccg tcgcggcacc atgtcgtttg actatatcga cgggtggtgg
     1261 gccgattgcg gcgagtccat cgactggtat ctacgcgcga ataacctcgt cgctcaagat
     1321 ggtgcgaatc atgtcgattg agtatggcgt tgtcgagtga agggaccgtc tacgcggtag
     1381 aagaaacgga tagcctgcat gacggaattg attatcgcga tggcgtttgc catcggcgtg
     1441 tcatttatct gctctttgct cgaggcggtg ttgtattcgg ttccgctcag tcacatcgag
     1501 acgcttgcgg aggaagaaag gccggccgga cggatcctac gcgagttgcg tgacgatatc
     1561 gatgcaccga tatcggcgat cctcacactc aataccatcg cgcacaccgt cggtgccgca
     1621 gttgccggag ccgcggcggc tgaagtcttc ggcagcaagt ggctcggtgc attttctgcg
     1681 gtcttcacgt ttctcattct tgtactctcc gaaatcattc cgaaaacagc cggagtaaca
     1741 tacgcgcgcg agcttgcac
//
LOCUS       DSCC01000198            2816 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024344, whole genome shotgun sequence.
ACCESSION   DSCC01000198 DSCC01000000
VERSION     DSCC01000198.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024344"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            139..1356
                     /locus_tag="ENN56_02475"
     CDS             139..1356
                     /locus_tag="ENN56_02475"
                     /inference="COORDINATES: protein motif:HMM:PF13527.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS74382.1"
                     /translation="MEATFASTSELERGYAVVSGHAFKKDTEFRNRKLQWMKRPGYRP
                     EHTRIIIDNGEVVAGVAIVEMHVRYGDATLKMGGISTVATDPDKQGRGYGRANMEDTV
                     RYMAEDGYDISFLLGIHDYYPKYGYRTALVWAPVVLNVAEAPSQMPEGWTARKIEESD
                     IPALDEVYRKTIGNCDLAVQRTEADWKWYFTFGRFGRANDRVIVNADNKVVGYFTLQG
                     RGDRMRVDELGVLDDVDAYAAVIAAMRDRGKDLFAREVEIYTVPDGGFARWALYRARG
                     KWSRSSDYAGGPMFRLFNAEQLFGKIGRTLAKRWEQAPRNTPEDAVTIRCPLGQVALI
                     PTGEGLRVRSGEESGTVVEMPHEALTELVLGFRPAVDILSDAGVTASESATRVLASVF
                     PVQIPFIPPTDHI"
     gene            1618..2349
                     /locus_tag="ENN56_02480"
     CDS             1618..2349
                     /locus_tag="ENN56_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_952839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HDS74383.1"
                     /translation="MSDTVDRAGVVVVPTYNERDNLVPLVDAIHAQRLNLDILVVDDN
                     SPDGTGDIADDLVRQGKVVHVLHRPGKLGLGSAYVDGFHWALARAYPLFFQMDADFSH
                     DPASLPVFVRAIENVDIVIGSRYISGITVVNWPLKRLLLSYFANMYARFITGLPIRDT
                     TGGFKCFRRHALEAINLKTISSEGYSFQIEMNFRAWRAGFRMKEIPIFFRDRRMGTSK
                     MDFKINREAVWMVWKLRLKALIGRE"
     gene            2301..>2816
                     /locus_tag="ENN56_02485"
     CDS             2301..>2816
                     /locus_tag="ENN56_02485"
                     /inference="COORDINATES: protein motif:HMM:PF13641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS74384.1"
                     /translation="MDGLEAATQSAYRERVNVTEAFPERVDQATNTSIDLTVLILSYN
                     VRDLLALALRTAQSAARGLSVELLVVDNDSPDDSADMVERDFPDVRLVRSGGNLGFAG
                     GNNLGMKVARGRYVLLLNPDVLVHPRAFAVLIDYMDANPDVGAAGGRVILGDGSIDHG
                     AKRGFPSPAAAF"
BASE COUNT          591 a          730 c          859 g          636 t
ORIGIN      
        1 cgtgtcctca atgaggcgaa tgtgcacgta tcggatgaga cgatacgtgt gctcgacgcc
       61 attttttcgc gatcaacagt ttggcggaca taattgtccc gcaaagagat tcctacctac
      121 atcagaggag tgccaagtat ggaggcgacg ttcgccagta catcagaact cgaacgggga
      181 tacgctgtgg taagcggcca cgcattcaag aaggataccg agttccgcaa tcgtaaactt
      241 cagtggatga agcgaccagg gtatcgtcct gagcatacgc gaatcatcat cgacaacgga
      301 gaagtcgttg ccggcgtggc gattgtcgag atgcacgttc gctacggcga cgcgacgttg
      361 aaaatgggtg gcattagtac cgtcgcgacc gatccggaca agcaggggcg cggatacggt
      421 cgggcgaaca tggaagacac ggttcgctac atggcggagg acggttacga tatatcattt
      481 ctccttggca tccatgatta ctatccgaaa tatgggtatc gaacagcgct tgtttgggcg
      541 ccggttgtgc tcaacgttgc ggaagcgcct tcacagatgc cggaaggctg gacggcacgg
      601 aaaatcgaag agtcggacat cccagcgctc gatgaggtat atcgaaagac catcgggaac
      661 tgcgaccttg cggttcagcg taccgaagcg gattggaagt ggtacttcac gttcggtcgc
      721 ttcggtcgcg cgaatgatcg cgttatcgtg aacgcagaca acaaggtcgt gggatacttt
      781 acgctacagg gtcgtggcga caggatgcgc gttgatgagc tcggtgttct cgacgacgtg
      841 gatgcgtacg cggcggtcat cgcggctatg cgtgatcgcg gcaaggatct ctttgcgcga
      901 gaggtcgaaa tctatacggt gccagacggc ggcttcgcgc gctgggcgct gtatcgcgct
      961 cgcggaaagt ggtctcgcag ctccgactac gccggtgggc cgatgttccg cttattcaac
     1021 gctgaacagc ttttcgggaa gattggccgt acgcttgcga aacggtggga acaagcgcca
     1081 cggaacacgc cggaagacgc ggttaccatt cggtgcccac tcgggcaagt cgcgttgatt
     1141 ccgacgggcg aagggctacg ggtacgttcg ggggaagagt cgggaacagt cgttgaaatg
     1201 ccgcacgaag cgttgaccga gttggttctt ggtttccggc ctgcggtcga tattctgagc
     1261 gatgccggcg ttacggcgtc ggaatcggcg acgcgagtgc tcgcgtcggt gttcccagtc
     1321 cagataccat tcattcctcc gacggaccat atctgattta tcgtcgcgtc tactaagcgg
     1381 gaggcgtcaa cgcggttgca gtagcgttgg cgcttcccgt atgatcagtt taccggttcg
     1441 gcgtccccac cacgaaccgc ctttgtttgt gtgtgtagaa catacttcgc attcgctcgt
     1501 agacatcgat atactgtacg gactggaacg gttttccatt gtcgacggta cgacaaaacg
     1561 ccattactaa ggattaccac agagcaccgt gtagccctga cctacgtaag agacggaatg
     1621 agcgatactg ttgatcgtgc gggtgttgtc gttgtcccga catacaatga acgagataat
     1681 cttgttcccc tcgtcgacgc gattcacgcg caacggttga acctcgatat ccttgtcgtc
     1741 gacgataatt cgccggatgg cacgggcgat attgcggacg atctcgtccg acaggggaag
     1801 gtggttcacg ttcttcatcg accggggaaa ctcgggttgg ggtccgcgta cgtggacggc
     1861 tttcactggg cgcttgcgcg ggcatacccg ttgtttttcc aaatggacgc ggacttctcg
     1921 catgatcctg cgtcgttacc ggtattcgtg cgtgcgatcg agaacgttga tatcgtgatc
     1981 ggctcgcgat atatcagcgg cataacagtc gtgaactggc cgctaaagcg tttgctgctc
     2041 agctatttcg cgaacatgta cgcgcggttt attacgggcc tgccaattcg agatacgacg
     2101 ggcggattca agtgtttccg gcgacacgct ctcgaagcga tcaatctcaa gacgattagt
     2161 tccgaaggat atagcttcca aatcgagatg aacttccgcg cgtggcgtgc cggatttcgc
     2221 atgaaagaaa ttcctatctt cttccgcgac aggcggatgg gaacgtcgaa gatggatttc
     2281 aagatcaacc gtgaagcggt atggatggtc tggaagctgc gactcaaagc gcttatcggg
     2341 agagagtaaa cgtgaccgag gcgtttccgg aacgtgtcga tcaagcgacc aatacgtcaa
     2401 tcgatcttac ggtgctcatt ctgagctaca acgtgcgcga tctcctcgcg cttgcgttgc
     2461 gcacggctca gtctgcggca cgaggtctct ccgtcgagct gctcgtcgtg gataacgatt
     2521 cgccggacga cagcgccgat atggtcgagc gggatttccc cgacgtgcgc ctcgtgcgaa
     2581 gcggtgggaa tctcgggttc gccggtggta ataacctcgg catgaaggta gcccggggac
     2641 ggtacgtcct ccttctcaat cccgatgtac tggtgcatcc gcgtgctttt gccgtgctga
     2701 tcgattacat ggacgccaat cccgacgtag gcgctgccgg tggacgcgtc attctcggtg
     2761 acggttcgat cgaccacggt gcaaagcgtg gttttccctc accggccgcg gcattc
//
LOCUS       DSCC01000199            2669 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026070, whole genome shotgun sequence.
ACCESSION   DSCC01000199 DSCC01000000
VERSION     DSCC01000199.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026070"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..381)
                     /locus_tag="ENN56_02490"
     CDS             complement(<1..381)
                     /locus_tag="ENN56_02490"
                     /inference="COORDINATES: protein motif:HMM:PF01553.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDS74385.1"
                     /translation="MSEEVGHTNKAIRYSIPVRIFYWFVRTLILIIATLLFRLQVRGK
                     EHQVKHGRAIFASNHIAWLDPLVVGVSIFRELSILGKKELFAIPVLNWIIRRLHAHPV
                     DRAGYSRGVLEMCKTLLENDHAILI"
     gene            complement(378..1061)
                     /locus_tag="ENN56_02495"
     CDS             complement(378..1061)
                     /locus_tag="ENN56_02495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDS74386.1"
                     /translation="MIVAIDGPAGSGKTTTARSVAERLGFVHIDTGAMYRAVTLQVLR
                     DGVSLDDDTTIIGTAKRVRIELKRTEDGVLRTLLNGDDVTHDIRESRVDMHVSRVSEI
                     AGVRDEIVRQQRELGRNVDAVMEGRDIGTVVFPDAEVKVYLVASVEERAKRRQKELTL
                     RGVNHDLADVLADIQRRDQHDSTRAVAPLKPAPDAIWIDTTSKSIDEQVDEVIRLVDK
                     RRRSEREAT"
     gene            complement(1058..1867)
                     /locus_tag="ENN56_02500"
     CDS             complement(1058..1867)
                     /locus_tag="ENN56_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDS74387.1"
                     /translation="MRIHDQRTSAESGDESSNGDVRLNAYIARCSATSRRGADRLIRY
                     RRVLVNGRPAEIGQRVDPNRDTVLVDNQKLTLPDTHTTILLNKPAGYLVSRRDPHHTR
                     TVYDLLPENLHDLVPIGRLDLDTEGLLLLSNDGDLVERMTHPRYQIPKTYRAIVRGRP
                     DESAMSRLRNGIEIDGEMTHPAEARIIGETAHGIELEFVLREGRKRQVRLMCRAIGHP
                     VVSLRRILFAGLSLEGVKPGTWRVLTNDEVDTLKNYRGTHARGAHARGAHA"
     gene            complement(1959..>2669)
                     /gene="dacB"
                     /locus_tag="ENN56_02505"
     CDS             complement(1959..>2669)
                     /gene="dacB"
                     /locus_tag="ENN56_02505"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:PF02113.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HDS74388.1"
                     /translation="VTVWRTVPDPVAYAGSVFRTALIDAGVTIGDSLIVATAPDSNTT
                     TLAVIHSASLDVLIRRFLKKSHNLTGEALLKHLGTTFRGTGSWECGLQATRSILHTFA
                     GIDSTTYRLADGSGLSRYTEISPRVLATLLRAASDEFTMAPEIFAAMPIAGVDGTLAR
                     RLSEDPIAGTVRGKTGSMTGVSSLAGVLETREGEKLVYCLLMNGFPGSSWAAREMQDR
                     IVSHLRELPCVSEQSVCP"
BASE COUNT          527 a          764 c          777 g          601 t
ORIGIN      
        1 gatgagaatc gcgtgatcgt tttcgagcag cgtcttacac atttccaata cgccgcgact
       61 gtatcccgcc cgatctaccg ggtgggcatg caatctgcgg atgatccagt tgagcacggg
      121 aatcgcgaac aattcctttt tcccgagtat cgaaagctca cgaaagatcg agactccgac
      181 taccagggga tccaaccacg cgatatggtt cgacgcaaat atcgcgcgtc cgtgctttac
      241 ttgatgctct ttcccgcgaa cctgcagtcg gaagagcagt gtggcgataa tgagaatgag
      301 ggttcgaaca aaccaataaa agattcgcac tggaatcgaa tagcgaatcg ctttgttggt
      361 atgcccgacc tcctcgctca tgtcgcctcc cgttctgagc ggcgtcgctt gtcgacgagt
      421 cgtataacct catcgacttg ctcatcgatc gacttgctcg ttgtatcaat ccaaattgcg
      481 tcgggtgccg gtttcagcgg ggcaaccgct cgtgtcgaat cgtgttgatc gcgtcgctgg
      541 atgtcggcaa gcacgtcggc aagatcgtga ttgactccac ggagcgtgag ctccttctgg
      601 cgtcgtttcg cgcgctcttc caccgatgcg acgaggtaga cctttacctc cgcatcggga
      661 aacacgacgg ttccgatatc gcgaccttcc ataacggcgt cgacgttgcg accgagttct
      721 cgttgctgtc gcacgatttc gtcgcgtact ccggcgattt cacttacacg tgagacgtgc
      781 atgtcgacac gcgattcgcg aatgtcgtgc gtgacatcgt cgccgttaag caacgtgcgt
      841 agaacgccgt cttccgtgcg cttgagttcg attctgacgc gtttcgcagt accgatgatc
      901 gtcgtgtcgt catcgagcga gacgccgtcg cgaagcacct gaagcgtcac ggcgcggtac
      961 atggctccgg tatcgatgtg tacgaacccg agtcgctctg cgactgaacg tgcggtcgtc
     1021 gtttttcccg atccggccgg accgtcaatc gcaacgatca tgcgtgcgct cctcgtgcgt
     1081 gcgctccccg tgcgtgcgtt cctcgatagt tcttcagtgt atcgacctca tcgttggtca
     1141 gcacacgcca tgttcccggt tttacgcctt cgagtgacaa accggcgaac aagattctgc
     1201 gaagcgaaac aaccggatgg ccgatcgcgc ggcacatgag ccgcacttgc cgcttccggc
     1261 cttctcgcag cacaaattcc agctcgatgc cgtgagcggt ttccccaatg attctcgctt
     1321 cggcgggatg tgtcatctcg ccgtctattt cgatgccgtt tcgcagccga ctcatcgcgg
     1381 attcgtcggg gcggccgcga acaattgcgc gatacgtttt cggtatctgg taccgtgggt
     1441 gcgtcatccg ttcgaccaga tcaccgtcgt tcgaaaggag caacagccct tcagtatcca
     1501 gatcaagacg cccaatcggc acgagatcgt gcagattctc cggcaacaga tcgtacaccg
     1561 ttcgcgtatg gtgcggatcg cgtcgggaga cgaggtaacc ggcaggctta ttcagcagga
     1621 tcgtcgtatg tgtgtcaggc agcgtgagtt tttgattgtc gaccagaacc gtgtcccgat
     1681 tcggatccac gcgctgaccg atttccgccg gtcgaccgtt caccagtacg cgccggtaac
     1741 ggatcagtcg atcggctccg cggcgcgatg tcgcagaaca gcgagcgata tacgcattca
     1801 gccggacatc gccgtttgaa gactcgtctc cgctttcggc ggaggttcgt tgatcgtgaa
     1861 tccgcaaaga gacgccgccc gtggggaata ccgtatctgc ttaccgttcg tttcgaatca
     1921 agggtaatgg tttaatgtag cggactgaga aaaaatcgtc aagggcaaac cgactgttcg
     1981 gaaacgcatg ggagttcgcg gaggtgtgag acaatcctgt cttgcatttc tctcgccgcc
     2041 cacgacgatc cggggaatcc gttcatgagg agacagtaca cgagcttttc accttcgcgc
     2101 gtctcgagta ctccggcgag gctactcaca ccggtcatcg aaccggtctt tccgcggact
     2161 gtaccggcga tggggtcttc cgagagcctt cgagcgagtg tcccgtcaac accggcgata
     2221 ggcatggcgg cgaagatttc cggcgccatg gtgaattcgt ctgaagcggc gcggaggagc
     2281 gtcgcgagca cccgggggct gatctcggta taccgtgaaa ggccggagcc gtcggcgaga
     2341 cggtaggtcg tagagtcgat gccggcgaag gtgtgaagga tactgcgcgt cgcttgcaac
     2401 ccgcactccc atgaccccgt tccacggaat gtagtaccca ggtgcttgag tagcgcttcg
     2461 cccgtgaggt tatgactttt tttcaagaac cgccgtatca gcacgtcgag tgatgccgag
     2521 tgtatgactg cgagcgtcgt tgtattgctg tcgggagcgg tcgctacgat gagagaatcg
     2581 ccgattgtga caccggcatc gatgagcgcg gtccggaata ccgaccctgc gtatgcgacg
     2641 gggtccggaa cggtgcgcca caccgtaac
//
LOCUS       DSCC01000200            2280 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032182, whole genome shotgun sequence.
ACCESSION   DSCC01000200 DSCC01000000
VERSION     DSCC01000200.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032182"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..344
                     /locus_tag="ENN56_02510"
     CDS             <1..344
                     /locus_tag="ENN56_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS74389.1"
                     /translation="MTYLGGERVTEGYNVMGVAKASLEMSMRYLANDLGPKNIRVNAL
                     SPGPLNTISARGVSGLRDLFTLMEERAPLRRNVSIEEVGDVAAFLLSDLSRAVTGQVI
                     FVDNGFNILGA"
     gene            346..1224
                     /gene="nadC"
                     /locus_tag="ENN56_02515"
     CDS             346..1224
                     /gene="nadC"
                     /locus_tag="ENN56_02515"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDS74390.1"
                     /translation="MGRIPGDQSEAIDLATLVRGALAEDIAEGDITSLATIPASRMAT
                     ARLIAKASGVIYGLEIARDVFLVVDPTIRFDANVREGAKVAVGDEIMTLSGPARSLLS
                     AERTALNFLQRLSGIATLSARYVEAVAGTDTKILDTRKTTPGWRTIEKAAVRAGGGHN
                     HRIGLWDMVLIKENHIRAAGSITKAILACRQWLTDNDRENVKIEIETTNKQEIEEALR
                     IGCDRIMLDNMTLSEMGEAVERIRREVPAPEIEASGNMTLDRVRAVAETGVDFISVGA
                     ITHSAPVLDLSLLFSS"
     gene            1226..2080
                     /locus_tag="ENN56_02520"
     CDS             1226..2080
                     /locus_tag="ENN56_02520"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDS74391.1"
                     /translation="MTTLHDPLTVDAVEGYLATRWLGRPCHSFGTVSSTMDVARRLAG
                     GGAPVGTMVVADAQTTGRGRQGRRWSSASGLGLWVSLIFPDDIASGVLAHVLACGVAE
                     GIERCGAEGVAVKWPNDVLVAETNTLDAASKNQPTMWPDAVVRSARKIAGILVERTGS
                     ARYSVVGIGVNVWHGMTDFPQDLADTATSVALCTGNPISRARLLAEVLGVMERRIDAV
                     RSGISLVDEWRRRSLVLGRLVCVERGDDRLIGRAAEIQPDGSLVVTVDGDSHVVGSGS
                     VRFLGLTE"
BASE COUNT          431 a          603 c          747 g          499 t
ORIGIN      
        1 gcatgacgta tctcggtggc gagcgtgtga ccgaggggta taacgtcatg ggcgtggcga
       61 aggcgtcgct ggaaatgtcg atgcggtatc tcgcgaacga tctcggtccg aagaacatcc
      121 gagtgaatgc gctgagcccg ggtccgttga atacgatctc ggctcgcggg gtcagtggat
      181 tgcgtgatct gtttacactc atggaagaac gtgcgccgct ccgtcggaac gtctccatcg
      241 aagaagtggg cgacgtcgcg gcttttctgt tgagcgatct atcgcgtgcg gtgaccggtc
      301 aggttatctt cgttgataac ggattcaata tcctcggagc ctgacatggg aaggattccg
      361 ggtgatcaat ccgaggcaat cgatctcgcg acgcttgtgc gcggcgctct cgccgaggac
      421 atcgccgagg gtgacatcac gagcctcgcg acgattccgg cctcacgcat ggcaaccgcc
      481 cgcctcatcg cgaaggcaag cggcgtcatt tatggtctgg aaatcgctcg cgacgtgttt
      541 ctcgtcgtcg accccacgat tcgattcgat gcgaacgtgc gcgaaggcgc aaaagtcgca
      601 gtcggtgatg agataatgac ccttagcggt ccggcgcgtt ctctcctttc cgccgaacgc
      661 accgcgctca actttctcca gcgtctctcg ggaattgcga cactcagtgc tcggtacgtg
      721 gaagccgttg ctggaacgga cacgaagatt ctcgacacga ggaaaacgac accgggttgg
      781 cgcacgatcg agaaggcggc cgtccgcgcg ggcggcggtc acaatcaccg tatcggccta
      841 tgggatatgg tgcttatcaa agaaaaccac atccgcgcgg cggggagtat cacgaaagcg
      901 attctcgcgt gtcgtcagtg gcttacggac aatgaccgcg agaacgtgaa aatcgaaatc
      961 gaaacgacga acaagcagga gatcgaggaa gcgcttcgaa ttgggtgcga caggatcatg
     1021 ctcgacaata tgacgctatc cgagatgggt gaagcggtgg agcggattcg tcgcgaagtg
     1081 ccggcgcctg agatcgaggc gtcggggaat atgacgctcg accgcgttcg cgccgttgcg
     1141 gaaaccggcg tcgatttcat cagcgttgga gcgattaccc atagcgctcc ggtacttgat
     1201 ctcagccttc tcttctcttc gtgatatgac gacgttacac gacccgctca ccgtcgacgc
     1261 cgtcgagggt tatctcgcga cgcgatggct cggcaggccg tgtcattcgt tcggcacggt
     1321 ttccagcaca atggacgtcg cacggcggtt ggccggcggc ggcgctcccg tcggcacgat
     1381 ggtcgtcgcc gacgcacaga ctaccggacg cggccgtcag gggcgtcggt ggagttcggc
     1441 gtcgggtctc ggtctgtggg tctccctcat ttttcccgat gatatcgcgt ccggtgttct
     1501 cgcacatgtg cttgcgtgcg gcgtggctga gggaattgag cggtgcggtg cggaaggcgt
     1561 cgcggtcaag tggccgaacg acgtgttggt tgcagaaacg aataccctcg atgcggcgtc
     1621 taaaaatcag cccaccatgt ggccggacgc agtggtgcga tcggcgcgca agattgccgg
     1681 aatactcgtt gagcggaccg gctcggcgcg atattccgta gtcggaatcg gggtgaatgt
     1741 ctggcatggc atgaccgact ttccgcagga ccttgctgat accgctacat ccgttgctct
     1801 gtgtacgggt aaccctattt cgcgtgcgag gttgcttgcg gaagtactcg gcgttatgga
     1861 gcgacgcata gatgcggtgc ggtccgggat ttcgctcgtc gatgagtggc gacgtcgctc
     1921 gctggttctg ggtagactgg tttgcgtcga acgtggcgat gaccgactca ttggtcgagc
     1981 tgctgagatt cagccggatg gttcgcttgt agtaaccgtt gacggggatt cacacgttgt
     2041 cgggagtggg tcggtcaggt ttcttggttt gacagaatga cgacgtacgt atactcaatc
     2101 gggtgtaccg gacgttccga ttgcatagag gatttgatcg ttgaatgaga ctcgtctcgc
     2161 gattggtttg cgcatcgcag tgcggaacga agcggtagcg cttcgtcgtg gcgcatggct
     2221 taccgatctt ttccgcgata agagcgtcgc taccggcgtc gagttggttc cggtggagac
//
LOCUS       DSCC01000201            1860 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042533, whole genome shotgun sequence.
ACCESSION   DSCC01000201 DSCC01000000
VERSION     DSCC01000201.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042533"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(553..1611)
                     /locus_tag="ENN56_02525"
     CDS             complement(553..1611)
                     /locus_tag="ENN56_02525"
                     /inference="COORDINATES: protein
                     motif:HMM:PF04055.19,HMM:PF13186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS74392.1"
                     /translation="MSMNVKEPLHFLRRSISIRKTWNVAKVSASYGISRLTKRPFVWG
                     VPPVLMIEPTNICNLKCPLCPSGNGTLRRERGFMDFDLYRRIIDDVRGRTHMILLWNQ
                     GESFIHPRFLDMIRYASDAGIYTYSSTNGHFLKDPDAVVGSGLDSMIISLDGATPETY
                     REYRVGGKFEKVIEGTKGLVAAKKRLGSKTPIVHIQFILFKHNEHEVGAVRALAEELG
                     VDKMTYKTAQIYEKEDIQVFLPENREFRRYEVEGDSFRLRNGKETVPNMCRKLWLQPV
                     INWDGSVTPCCFDKNGDFTMGMYDGDGRESSFERLWNNDAFNQFRRRVLTDRAAIDMC
                     RNCTEGVKYNREEDIHVI"
BASE COUNT          372 a          543 c          533 g          412 t
ORIGIN      
        1 actattaacc attccggcac gatacttcac ttccgacacg cgtggagcgg gcccgacgcg
       61 cttgccgtat gtgatatcga cggtgagaac ttccgaggca tcggctttca ggattttgcg
      121 caccggatgt ggctcggcac gacgaaccgg ctccaaggac cgctgctacc gcccgggcgc
      181 ggcattgtgc aggtcgaact tgatgacacc gagccgacgc cgatctgctc aggtccatac
      241 ttctggcatt cagcctcgag tatcgacggt gaatggatcg ttgcagatac gaactggccg
      301 gaacagggga ttctgctggt gcacgtcgcg agcggacggt atgcgccggt tacgatgccg
      361 cacgcgtgcc ccggcgcggc gcagtatacg catccgcacc cgtcgttcga ccgcaccggt
      421 tcccgagtga tctacacggt gaatcgcggc gggttgtcgc aggtgcacat cgcgacgata
      481 ccggacgaac tgcgccacga actcgctacg ggagcgctta cgaatcgact gcggctgcgg
      541 taagctgcgg aactaaatga cgtggatgtc ttcttcgcgg ttgtacttga cgccttcggt
      601 acaattgcgg cacatgtcga tcgcggcgcg atctgtgaga acgcgacggc ggaattgatt
      661 gaacgcgtcg ttattccata gccgttcaaa tgagctttcg cgcccgtcgc cgtcgtacat
      721 gcccatggtg aaatcgccat ttttatcgaa gcaacatggg gtgactgaac cgtcccaatt
      781 gatcaccggt tgaagccaga gcttccggca catattcggc acggtctcct tcccattccg
      841 cagccggaat gagtcgccct cgacctcgta tcgacgaaat tcccgattct ccggaaggaa
      901 gacttggatg tcttcctttt cgtagatctg cgccgtcttg taggtcatct tgtcgacgcc
      961 gagttcttcg gcaagcgcgc gtacggcgcc gacctcgtgc tcgttgtgtt tgaacagaat
     1021 gaactggata tgtacgatcg gcgtcttcga gccgaggcgc ttcttcgccg caacgagccc
     1081 tttcgtgcct tcgatcacct tctcgaactt cccgccgacg cggtactcgc gataggtctc
     1141 cggtgttgcg ccatcgaggc tgatgatcat gctgtcgagg ccgcttccga ccaccgcgtc
     1201 cgggtccttc aggaagtgac cgttcgtcga cgagtacgtg taaatgcccg cgtcggaagc
     1261 gtaccgaatc atgtcgagga agcgtgggtg aatgaacgac tcgccctgat tccagaggag
     1321 gatcatgtgc gtgcgcccgc gtacatcgtc aatgatgcgc cggtacaagt cgaaatccat
     1381 gaatccgcgt tcgcggcgaa gtgtgccgtt accgcttgga cagagagggc atttgaggtt
     1441 gcagatattc gtcggttcga tcatgagaac cggcgggaca ccccatacga acggacgttt
     1501 cgtaaggcgg gatattccgt agcttgctga cactttcgcg acgttccacg tcttccgaat
     1561 actgatgctc cgacggagga aatgaagcgg ctccttgacg ttcatcgaca tgtatagctc
     1621 aatccagcat agcccttatg attgtcacca tatcctatac gtaacgccca gagagaacgc
     1681 agtccagtcc cgtgtactcg ttacaccggc atcctcggtc ttgcggatcg tctgcgatac
     1741 ggtgccgctt gcgaagaaat cgcgcgcgaa ctcccaccga acaccgaagc ggtattcata
     1801 ggtgcgcgtc acgtcgccaa agagaaactc tttcgcgggc gcatccgtgt cgtactgttc
//
LOCUS       DSCC01000202            2885 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023558, whole genome shotgun sequence.
ACCESSION   DSCC01000202 DSCC01000000
VERSION     DSCC01000202.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023558"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(188..1840)
                     /locus_tag="ENN56_02530"
     CDS             complement(188..1840)
                     /locus_tag="ENN56_02530"
                     /inference="COORDINATES: protein motif:HMM:PF04909.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74393.1"
                     /translation="MDGITYFDCYASIGKMGPKDELTPWTKEALLDEMLHCGIHAALV
                     ENSVAREYDPAYGNQKLIGEIQNERRLFPAWVVLPHQTGEMPIGPELVEQMLRHDVRA
                     ARIYPKGQNFALDERTCGELFSALEAERILLSIDFGQASPADIADMCTRHPDLPVLIT
                     DISWANTRFLYPVMDQCPNIHVEFSTFQANYAIERLGERYGFERLLFGTNALSKSLGA
                     AKSFIDYADIDDDARRKVAGENLARLLRLPKLPGGYRPDARAQHSVPGRPVSPTAAFR
                     AQKSSRILRLVKEGKALDHITVIDAHSHIVHDGGQGVGYIPMPQGDAENMVARNRRMG
                     IDAFMTSSWLGIWADCEDGNRIVRRAIEQYPNDVIGYATLNPNYLSEAEFARWLRVVY
                     EEWGFKGMKPYHPRMRLSYNSPKYDQWYAYGDEHRLICLLHSSGGGFLNEVRDIAKRY
                     PNISFLLAHTGGDFQTARERLPIVREFDNVYLEITLTPVTYGVIEYMVNEVGAERVVF
                     GTDAPMRDPIPQFGWMCYSRCSDDELELMLGKNIERLLDRVR"
     gene            complement(1894..2883)
                     /locus_tag="ENN56_02535"
     CDS             complement(1894..2883)
                     /locus_tag="ENN56_02535"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:PRK01372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDS74394.1"
                     /translation="MKTGTVITNNLDRIRYRVLPIQIRRDGGWLIPDAPLTGSDSWMP
                     ADETTPPVPEHTTVPAEELLRRGIDVVIVALHGRFGEDGSIQGLLDILGIPYTGSGVL
                     GSALAMDKIRAKSLVSFAGILTPKWTVVEEADWQRRSDQIVADIGRDFGFPCVAKVPE
                     EGSSFGMGIPQTAEELRAVIDAYVAARGHMLVEEYIKGVEITGSVLGAEPGGEPTALP
                     LTEIVPKTSKFFDFEAKYTKGASDEITPARLDDALTIRAKQIAVTAHRVLDCGTLSRT
                     DMIVRDCDIYYLETNTIPGMTETSLFPQAAAAAGISFPELLDRQIVLALARRQ"
BASE COUNT          560 a          836 c          865 g          624 t
ORIGIN      
        1 atggaggaac tcggcgcgtc tgcacctcgt ccgataaaca cttcgcagtg gagaaacgcc
       61 gtcggattct gataatggac aaaagaatcg gtttacccag tattaagcgt gtttcatttt
      121 tgggattttg cacgagccga ctgtactgat cctgcagaga agtccgtgcg gtgtctccct
      181 tctgtaatca gcgaacacga tccagtaatc gctcgatgtt cttcccaagc atgagttcga
      241 gctcgtcgtc ggagcagcgg ctgtagcaca tccatccgaa ctgcgggatt ggatcgcgca
      301 tcggcgcgtc cgtgccgaag acgactcgtt cggctccgac ctcgttcacc atgtattcga
      361 tgacgccata cgtcaccggc gtaagggtaa tttcgagata gacgttatcg aactcgcgga
      421 caatgggcag ccgttcacgc gccgtctgga agtcgccgcc ggtatgggca agtaagaacg
      481 agatattcgg ataccgcttc gcaatatcgc gcacctcgtt gagaaaaccg ccgccggacg
      541 aatgcagcag acagatcagg cgatgttcgt cgccgtatgc gtaccactga tcatacttcg
      601 gtgagttata ggagaggcgc atgcgtgggt ggtatggctt catgcctttg aagccccact
      661 cctcgtagac tacacgaagc caccgcgcga actcggcttc gctgaggtaa ttcggattga
      721 gggtcgcgta gccgatcaca tcattgggat actgctctat ggcgcgccgc acgatgcggt
      781 tgccgtcctc gcaatccgcc cagataccga gccacgaact cgtcatgaac gcgtcgattc
      841 ccatgcgccg gtttcgcgca accatgttct cggcatcacc ctgtggcatg gggatgtagc
      901 caacgccttg gccgccatcg tgcacgatgt gcgagtgcgc gtcgatgacg gtgatatggt
      961 cgagcgcctt gccttccttt acaaggcgaa ggatgcgact cgatttctgt gcgcggaacg
     1021 ccgcggttgg ggatacgggg cgacccggta cgctgtgctg cgctcgtgca tcggggcgat
     1081 atccgcccgg gagcttcgga aggcggagaa gccgcgcgag gttctcgccg gcgaccttgc
     1141 gccgagcgtc gtcgtcgata tccgcgtagt cgatgaatga ttttgccgcg ccgagacttt
     1201 tcgagagcgc gttggttccg aagagaagcc gttcgaaccc ataccgctca ccgagtcgtt
     1261 cgatggcgta gttcgcttga aacgtgctga attcaacgtg aatgttcgga cactgatcca
     1321 tgaccgggta gagaaagcgc gtattcgccc agctgatatc ggtgatgagc accggcagat
     1381 cggggtgccg cgtgcacata tcggcaatat ccgccgggga cgcctgtccg aaatcgatgc
     1441 tcagtaggat ccgctcggct tcgagcgcgc tgaataactc gccgcaggtt cgttcgtcga
     1501 gcgcgaagtt ctgtcccttc ggatagattc gcgccgcacg gacgtcgtgc cggagcatct
     1561 gttcgacgag ctccggtccg atcggcattt caccggtttg atgtggcaga acgacccatg
     1621 ccgggaagag ccgccgctcg ttctggattt cgccgatgag cttctggttc ccatacgccg
     1681 gatcatactc gcgcgcgaca ctgttctcga cgagcgccgc gtgtatgccg cagtgcagca
     1741 tctcgtcgag aagcgcctct ttcgtccacg gcgtcagttc gtctttcggc cccatcttgc
     1801 cgatggatgc gtagcaatcg aagtaggtaa tgccgtccat ctatggttcc tctttcaagc
     1861 gatgttcaaa atggtctatt tgcgttgcgc caactattgg cggcgcgcca acgcaagcac
     1921 aatctgtcgg tcgagtaact ccgggaagct gatgcccgcc gccgctgccg cttgtgggaa
     1981 aagcgatgtc tcggtcatgc cggggatcgt gttggtctcg aggtagtaga tatcgcagtc
     2041 gcggacgatc atgtcggttc ggctcagtgt gccgcaatcg agcacgcgat gggccgtgac
     2101 ggcaatctgc ttcgcgcgta tcgtgagcgc gtcatcgagt cgggcagggg tgatctcgtc
     2161 cgaagcgcct ttcgtgtact tcgcttcaaa gtcgaaaaac ttcgaagtct tcggtacaat
     2221 ctcggtaagc ggaagcgcag tcggttcgcc acccggttcg gcgccaagca cggaaccggt
     2281 gatctcgacg cccttgatgt actcctcgac gagcatatga ccgcgcgcgg caacgtatgc
     2341 gtcgatcacc gcgcgaagct cttcagcggt ctgcgggatg cccataccga agcttgatcc
     2401 ctcctccggg accttcgcga cacaggggaa gccgaaatcg cgtccgatgt cggcaacgat
     2461 ctgatcagac ctgcgttgcc agtccgcttc ctcgacgacc gtccacttcg gtgtcagaat
     2521 accggcgaac gaaacgagcg acttcgcacg gatcttgtcc atcgcaagcg cggagccaag
     2581 cacaccagag ccggtatacg ggatcccgag tatgtcgagc agcccctgga tgcttccgtc
     2641 ctcgccaaac cgtccgtgca gcgcgacaat caccacgtcg atgccacgtc ggagcagctc
     2701 ttcggctggt acggtggtgt gttcgggtac cggtggcgtc gtttcgtcgg cgggcatcca
     2761 cgaatcggaa cccgtcagcg gtgcatcggg tatgagccaa ccgccgtcac ggcgaatttg
     2821 aatcgggagt acgcgatatc ggatgcggtc aaggttattc gtgatcactg tgccggtctt
     2881 cagtg
//
LOCUS       DSCC01000203            1770 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045463, whole genome shotgun sequence.
ACCESSION   DSCC01000203 DSCC01000000
VERSION     DSCC01000203.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045463"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1770)
                     /locus_tag="ENN56_02540"
     CDS             complement(<1..>1770)
                     /locus_tag="ENN56_02540"
                     /inference="COORDINATES: protein motif:HMM:PF07715.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS74395.1"
                     /translation="LVLIISTASIVCAIPVGKFSGRVTDENGVPLKDVTILLDGTDLN
                     ATTDSDGNYVVLQVPPGTYGIVARRAGFRHIRKPAVAVYADRTVRVDFSLPLAGIEIE
                     TETVPTDPPLITPDVTSTRSYIDLRQYRRIPTSSIVDVLAFESGVRKTPNNELAIRGF
                     HPNEVVFQIDGFTWMNPLEGRPYSEVNVAFIEQVQIVSGAYVAGKPGRGAIINIVTRD
                     PANMATLSGDARYTLPHRKHFGPDAYGSDQYDRLLYLNAGTSTGGRLTNQSSIPPGGP
                     VSGWDVVDSDKPVYRVQSATDALDTQPVFVGWIQATAEVNAGTRALGDSLIDAHRGSW
                     TPETLKQVWSHRHRAWSYADRGDLFLDLALASPSYLIPRTNFAAGYRHNRTVLPVPAV
                     VQDYTNRALTASLHSRVIPRVTIDGYGQFEMIETTANGVSALGGPGTGTGVLERFVGV
                     ADPMAPAEVYESSPNDWMNTLAHTLDDHGGPAGLNKYNLWANDPYTEQLWGGGLRLQH
                     RVSNRVFYGISVEHLRATVEMKPAERRGSNSNGHRTDRNFETVSLGGDDVAFLDESPW
                     GFDPGSYAGLDVVGGYYLSGGGY"
BASE COUNT          424 a          515 c          472 g          359 t
ORIGIN      
        1 cgtatccacc accagagaga tagtagccac cgacgacatc gagtccagcg tacgagcccg
       61 gatcgaaacc ccacggactc tcgtcgagga acgcaacatc atcgccgccg agcgaaacgg
      121 tttcgaagtt cctatccgta cgatgaccgt tgctgttcga tcctcgacgt tcggcaggct
      181 tcatctccac ggttgcacga agatgctcga cgctgatccc atagaacaca cggttcgata
      241 cgcggtgttg cagacgcaaa ccaccgcccc acagttgttc cgtgtacgga tcattcgccc
      301 acaagttgta cttgttcaac cccgccggtc caccatgatc atcaagcgta tgcgcgagcg
      361 tgttcatcca atcgttcggc gatgactcgt atacctcagc cggtgccatc ggatcggcaa
      421 caccgacaaa tcgttcaagg actccggttc cagttcccgg accgccaagc gccgatacac
      481 cgttcgcggt agtttcgatc atctcgaatt gaccgtaccc atcgatcgtc actctcggta
      541 tcacccgcga atgaagggag gccgttaaag cacggttcgt gtaatcctgc acgacggcgg
      601 gcacgggaag taccgttcga ttgtgtcggt atccggccgc gaaattcgtg cggggaataa
      661 gatacgacgg cgacgcaagt gcgagatcga gaaacagatc accccgatcc gcatacgacc
      721 acgctcgatg acgatgcgac cacacctgtt tcaatgtttc gggagtccaa cttcctcggt
      781 gtgcgtcgat gagcgaatcc ccaagcgcac gagttccagc atttacttcc gctgtcgcct
      841 gtatccaacc gacgaagacc ggttgcgtat caagtgcatc cgtcgctgac tgaaccctgt
      901 ataccggttt gtcggaatct acgacatccc aacccgacac cggcccacca ggaggtatcg
      961 atgactgatt ggtaagccga ccgccggtgg acgtccccgc gttgagatac aacaaccggt
     1021 cgtactgatc ggaaccatag gcatccggac cgaaatgttt gcggtgggga agtgtatatc
     1081 gagcgtcacc ggaaagcgta gccatgttcg ccggatcgcg agttacgata ttgatgatcg
     1141 ccccacgccc cggcttccca gcgacgtacg cgcccgaaac aatctgcacc tgttcgatga
     1201 aagcgacgtt cacttcagaa tacggccgac cttcaagcgg attcatccat gtaaagccat
     1261 caatctgaaa gacaacttca ttcgggtgaa acccacgaat cgcgagttca ttattcggcg
     1321 ttttccggac accggactcg aacgccagca cgtcaacgat agagcttgta ggaattcgac
     1381 gatactggcg tagatcgatg taggagcgag tcgaggtaac atcaggagtg atcagaggcg
     1441 gatcagtcgg aaccgtctcc gtttctatct cgatgccggc aaggggcaat gagaaatcta
     1501 cacgaaccgt ccggtcggcg tagacggcta cggccggttt gcggatatga cgaaaaccgg
     1561 cacgacgcgc cacgataccg taggtcccgg gcggtacttg gagaacgacg taattcccgt
     1621 ctgagtctgt cgtagcgttg agatcggtgc cgtcgaggag aatcgtgaca tctttcagcg
     1681 gaacgccatt ttcatcggtc acacgccccg aaaatttccc gactggtatt gcgcaaacaa
     1741 tcgatgcggt agagatgata agtacgagaa
//
LOCUS       DSCC01000204            1542 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054763, whole genome shotgun sequence.
ACCESSION   DSCC01000204 DSCC01000000
VERSION     DSCC01000204.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054763"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..387)
                     /locus_tag="ENN56_02545"
     CDS             complement(<1..387)
                     /locus_tag="ENN56_02545"
                     /inference="COORDINATES: protein motif:HMM:PF01546.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDS74396.1"
                     /translation="MILAELIGKINTKRLATRALDMLRIPSPTGQTRVVAEYFSEACR
                     SLGGAVTVIDEIPDAPAEYDAPSVAALWQFGDGPTLQIDGHTDTIDMPHSPAELRDGF
                     LTGRGAVDMKASVAAAIEAVNVISDTG"
     gene            504..1466
                     /locus_tag="ENN56_02550"
     CDS             504..1466
                     /locus_tag="ENN56_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDS74397.1"
                     /translation="MKESGLDAAAKVAVRQCLAVQPNEQVVIVFDEPCCSIARALWRA
                     SCDVGAETLLIEMTPRVENGSEPPAPVTAAMRSANVVFAPASKSITHTEARRSATAAG
                     ARVATLPGITESIFSRTLVADYEAIRRRTETVASRIRNRNTVRITTPAGTDITFSIAG
                     RDVMLDTGILRQPGVTNLPAGEAFLAPVEGTGEGVIMIDGSIAQSGVLTSPITVEVRN
                     GLVSTISGHPYANTLLRAMEAAGPEARNLAELGIGTNDSARLVGHVLEDEKILGTVHL
                     AFGDNRSMGGSVRVPFHQDGILLSPSLWVDDEQVMRDGEFVGEK"
BASE COUNT          346 a          422 c          423 g          351 t
ORIGIN      
        1 ccctgtatcg gatatcacgt tcactgcttc gatcgccgcg gcgactgacg ctttcatatc
       61 cacagcgccg cgaccagtaa ggaaaccgtc tcgtagttct gccggagaat gcggcatatc
      121 aatcgtatcg gtgtgcccgt cgatctggag cgtcggtccg tctccgaatt gccataacgc
      181 agcgacgctt ggtgcatcat attccgccgg cgcatcggga atctcatcga taacggtaac
      241 cgccccaccg agactccgac acgcctccga aaaatactct gcgacgactc gtgtttgccc
      301 cgtcggactg ggaatccgaa gcatgtccag cgcgcgtgtc gcgagtcgtt ttgtgttgat
      361 ctttccgatt aattcagcaa gtatcatagg tacaacccct ttatagtgct actattaagc
      421 agcggaagcg ttgatatcga aaaactgcgt gcttccgaag ttcgcgtaga tagctgtacg
      481 gcgcaatacg agcaggagga aggatgaaag aatctggttt ggatgcggcc gccaaagttg
      541 ccgttcgtca atgcctcgcc gtccaaccaa atgaacaggt cgttatcgtc ttcgacgagc
      601 catgctgttc aattgcacgt gctttgtgga gagcatcgtg cgatgtcggc gccgaaacgt
      661 tgctcataga aatgactcca cgtgtcgaaa acggttcaga accaccggca cccgttaccg
      721 ctgcgatgcg atccgcaaac gtcgttttcg cgcccgcgtc gaaatccatt actcacacgg
      781 aagcgcgaag gtccgccact gcggctggtg cgcgcgtcgc cacgctaccc ggtattaccg
      841 aatcgatctt ctcacgtact ctcgtcgccg actacgaggc gattcgccga agaaccgaaa
      901 cggttgcgtc gcgaattcgg aatagaaaca ccgttcgaat caccacaccg gcaggcacag
      961 atattacgtt ttcgattgcc ggccgtgatg ttatgctcga tacgggaatc cttcgccaac
     1021 caggagtaac gaatcttcca gctggtgaag catttctcgc acctgtcgaa ggaacgggcg
     1081 aaggcgtcat tatgattgat ggctcgatcg cgcagagcgg cgttctcacc tcaccgataa
     1141 cggtcgaggt gcgcaacggc ctggtatcga ctattagcgg gcatccatat gcaaatacgc
     1201 tacttcgtgc gatggaagcg gccggtcctg aagcacgaaa tcttgcagaa ctcggtatcg
     1261 gcacgaatga ctccgcgcga ctcgtcggac atgttctcga agacgagaag attctcggca
     1321 cggtgcatct tgctttcggc gataatcgat caatgggtgg aagcgtacgc gttccgttcc
     1381 atcaggacgg tatcttgctc agcccgtctc tgtgggttga tgatgagcag gtgatgcgcg
     1441 acggcgagtt tgtaggggaa aaatagtgga ttgttgaata caccgtcggt agttgacgcg
     1501 ttttaccggt tgtaacgagt ttttcgagat accggcgcac cc
//
LOCUS       DSCC01000205            2355 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030844, whole genome shotgun sequence.
ACCESSION   DSCC01000205 DSCC01000000
VERSION     DSCC01000205.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030844"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            301..516
                     /locus_tag="ENN56_02555"
     CDS             301..516
                     /locus_tag="ENN56_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDS74398.1"
                     /translation="MSVGTVKWFSEENGFGFIMQEDGEDVFVHRTQIEGQECHRVLYC
                     GDEVEYETVAGTKGLSARHVHVLSRIE"
     gene            586..1053
                     /locus_tag="ENN56_02560"
     CDS             586..1053
                     /locus_tag="ENN56_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDS74399.1"
                     /translation="MPINQVTQFLDEHKIRYVTITHSPAYTAQEIAAHSLLPGDELAK
                     TVIVKLDDVPVMAVVPASCRIDLDKLRWAAGADRAELAVEAEFVAMFPDCRTGAMPPF
                     GNLYGLPVWVDERLTRNRYIDFNACSHTELMRMRVTDYLRLVRPTEAAFSEPE"
     gene            1223..>2355
                     /locus_tag="ENN56_02565"
     CDS             1223..>2355
                     /locus_tag="ENN56_02565"
                     /inference="COORDINATES: protein motif:HMM:PF16216.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74400.1"
                     /translation="MNLTEVFPTIPQVKRLLVSRVPNKSHAADFWVWFRSFQGLVNKR
                     EPHLYTIRDVAGPGKTNHSLEKYPFVGQYEDHWLNYYAETFGLPVENCDDVDELIERY
                     KDIVNGYVVYDNTDVIQTQNLAINQCSLEGVLPIAPDQEDWMIRHGIPKRDDLRGRFA
                     DDWDAAEWAIDNQWPHTYKKIYANFCIHRPVGYAYGHDLQDFIVMHRGMALDLPRTRP
                     MRRSLMLYRRMLESGDAPGVQMNWHCAWEQEKEYVVEAAKHGYFVLCSSGTPNLSIHE
                     GVGDPSKSYEQPMPKREDCRAEKGKVYVCFYNSDGDATWAMNNLHHGNWAEKDRGDFK
                     FSWGLLPLMVKLMPGMLQYYHETKTPNDKFWGPSSGSAYTYSWA"
BASE COUNT          497 a          666 c          712 g          480 t
ORIGIN      
        1 gatcgggtgc atcgggtggt agagttttat agtttgacat atatcgaaat ataaacgtgc
       61 atcggctgat gtcaagtgat ataacgtgtt cccgtacgac gagtcgcgtg cggtccgcga
      121 caccgtttaa ccggtgggaa tgcccatatg atggggtgtt atcggcgtgt tattgccgac
      181 tcggtcgcga cgcggtatac tttcgggtgg gaggtttttc gcgacccgtc gtctcgagag
      241 ggaggtgaca cgtagcgctc gcgttttcca aataccgccg acggtcacgg gagtttgtcc
      301 atgtcagttg gaaccgtgaa atggttcagt gaggagaacg gattcgggtt catcatgcag
      361 gaggacggag aggacgtctt cgtgcatcgt acgcagatcg aggggcagga gtgccatcgg
      421 gttctctact gcggcgacga ggtggaatac gaaaccgtgg cgggaacgaa gggtctctcc
      481 gcacggcacg tccatgtcct ctctcgtatc gagtagcgaa cggcaccacg tcatctctct
      541 tcgggcaccg ctctcccaca cttgaaagag gaaagggggc cgacgatgcc gatcaatcag
      601 gtcacgcagt tcctcgatga gcacaaaatc cgctatgtga cgatcaccca ctcgccggcg
      661 tacaccgcgc aggagatcgc cgcgcattcg cttcttcccg gcgacgagct tgcgaaaacc
      721 gtcatcgtaa agctcgacga cgtcccggtc atggcggtcg ttcctgcgtc ctgccgtatc
      781 gatctcgaca agttgcgatg ggccgcggga gccgatcgcg ccgagctcgc tgtggaggcg
      841 gagttcgtcg cgatgttccc cgactgccgg acgggcgcga tgccgccgtt cgggaatctg
      901 tacggcttgc cggtgtgggt tgacgagcgg ctgacgcgga atcgctacat cgatttcaac
      961 gcatgctcgc atacggagct catgcgaatg cgggttaccg attacctacg cctcgtgcga
     1021 ccgacggaag cggcgtttag cgaaccggaa taagcgcggc agcgtgtcaa acgcgtggcg
     1081 cgtcgcgttc gacacttgta caatactttt tcgcacgcgc gccgtgtacg gggtaggtac
     1141 gcggcgcgct tctttccacc ttgtactcca atcttcgtac gtcaattttc ccagtgccgg
     1201 tttttcctca tgaggtccac ggatgaatct gacagaggta tttcccacca ttccgcaggt
     1261 aaaacgcctg cttgtatcgc gtgtaccgaa caaaagccat gccgccgatt tctgggtgtg
     1321 gttccgctcg tttcagggac tcgtgaacaa acgcgaaccg catctctata ccattcgtga
     1381 cgtcgcgggt cccggaaaga cgaatcactc gctggagaag tatccgtttg tcggccaata
     1441 cgaagaccac tggctcaact actacgcgga gacctttggt ctgccggtcg aaaactgcga
     1501 cgatgtcgac gagctgatcg agcggtacaa ggacatcgtc aacggctatg tcgtctacga
     1561 caacacggac gtcatccaga cgcagaatct cgcgatcaac cagtgcagtc tcgaaggtgt
     1621 tcttccgatt gcgcccgatc aggaagactg gatgattcgc cacgggatcc ccaagcgcga
     1681 cgacctgcgc ggcagattcg ccgacgactg ggatgccgcg gaatgggcga tcgacaacca
     1741 gtggccgcac acatacaaaa agatttacgc gaacttctgt atccatcgac cggtcgggta
     1801 tgcgtacgga catgacctgc aagacttcat cgtcatgcac cgtggaatgg cgctcgacct
     1861 gccgcgcact cgtccaatgc ggcgctcgct catgctgtac cgccggatgc tcgaatcggg
     1921 agacgcgccc ggcgtccaga tgaactggca ctgcgcgtgg gagcaggaga aggagtacgt
     1981 cgtcgaggcg gcgaagcatg gctacttcgt cctgtgcagc tcggggactc cgaacctgtc
     2041 gattcacgaa ggcgtcggcg atccgagcaa gtcgtatgag cagcccatgc cgaagcgcga
     2101 agactgccgc gccgagaagg ggaaggtcta cgtctgcttc tacaattccg acggcgacgc
     2161 gacatgggcg atgaacaacc tccatcacgg aaactgggcc gagaaagatc gcggagactt
     2221 caaattcagc tgggggctgc tcccactcat ggtgaagctc atgccgggaa tgctccagta
     2281 ttaccacgag accaagacgc cgaacgacaa gttttgggga ccgtcgtccg gctcggccta
     2341 cacgtactcg tgggc
//
LOCUS       DSCC01000206            1767 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045579, whole genome shotgun sequence.
ACCESSION   DSCC01000206 DSCC01000000
VERSION     DSCC01000206.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045579"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="ENN56_02570"
     CDS             complement(<1..1634)
                     /locus_tag="ENN56_02570"
                     /inference="COORDINATES: protein motif:HMM:PF05592.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74401.1"
                     /translation="MTASSPMNGRSSWVWIDYAAYDMGSWSAPTNTGDSPFLMGYFRR
                     RFTAPANARLTLHVSADSRYILWCNGVAVGRGPAKGDVRHQFFETYDLSAHLRDGENV
                     LVAQVVSFARARAFPSQSGAPNSIMTAAWLFAAEGDVVDANGNVVDTVDTDARWVAIP
                     DRAYRWRHRENWGTYLGMLEEVHGAEYPWGWQQADFDDAAWKPVQALHPTVSDAEVTG
                     LDMHVPQVLTPRTIPMLEETPMRFDGAAHVRLIHPTGTEGSAAECRAWTKAVIALIRD
                     DRAVRIPANTKLSVTLWCDALQTGFPEIAVEEGRGTRITATYAEALTYGDGAIDQENW
                     RDFKGNIEPRHAPDEGVVMGYWDEYISGGGAESWEPILWRTFRYVRIEIETAEEPITV
                     TQLSYRFTGYPYEERASFRSSDPGHARMWELSWRTARLCAHETYEDCPYYEQLQYAGD
                     TQVQARIGYTVAADPRLARQAIRHFDWSREASGPPQSRYPSRNPQYIPTWSMIWVMLV
                     RDYWWHTGDVEETANRLPGISSTLSWFERYENSDGLL"
BASE COUNT          360 a          554 c          505 g          348 t
ORIGIN      
        1 agcaggccgt cggagttctc gtacctctcg aaccacgaga gtgtcgacga gataccgggt
       61 aaacgattgg cggtttcttc aacgtcgccc gtatgccacc aatagtcgcg cacgagcatc
      121 acccagatca tgctccacgt ggggatgtac tgcggattgc ggctcggata ccggctctgc
      181 ggaggtcccg acgcctcacg cgaccagtcg aagtgccgaa tcgcctgccg agcgagccgt
      241 ggatcggctg cgacggtata cccgatgcgt gcctgaactt gggtatcgcc tgcgtactgt
      301 agctgctcgt agtatggaca gtcttcgtac gtctcatgcg cacagagtcg cgccgttcgc
      361 catgaaagct cccacatgcg cgcatgaccc gggtccgatg agcggaacga cgctcgttct
      421 tcgtacggat acccggtgaa tcgataactc aactgcgtca cggtaatcgg ctcttcggca
      481 gtttcaatct cgattcgaac gtagcggaac gtcctccaga gtattggctc ccacgattcg
      541 gccccgccac ccgagatgta ctcgtcccaa taccccatga caacaccttc gtccggagcg
      601 tgacgcggtt cgatattgcc tttgaaatcg cgccagtttt cctgatcgat ggcgccatcg
      661 ccgtacgtga gcgcttccgc gtatgtcgcg gtgatacgcg ttccgcgacc ctcctccacg
      721 gcgatttccg ggaatccggt ctgcaacgca tcgcaccaga gcgtcacgct caacttcgta
      781 ttcgccggaa ttcgaaccgc gcggtcatcg cgtatgagcg ctataacagc cttcgtccac
      841 gcacgacact ccgcagcact tccctctgta ccggtgggat gaatcagccg tacatgcgcc
      901 gcaccgtcga aacgcatcgg cgtttcttcg agcatcggaa tcgtgcgggg tgtgagaacc
      961 tgcggcacgt gcatgtcgag gcccgtcact tcagcatcgg atacggtagg atgaagcgcc
     1021 tgcaccggtt tccatgctgc gtcgtcgaaa tccgcctgtt gccatcccca tggatactcg
     1081 gcaccgtgca cctcttcgag cataccgaga tacgtgcccc agttctcgcg gtgtcgccaa
     1141 cgatacgcgc gatcaggaat ggcgacccag cgtgcgtcgg tgtcgaccgt atccacgaca
     1201 ttcccgttcg catcgacgac atcgccttcc gctgcaaaga gccacgcggc ggtcatgatc
     1261 gagttcggcg caccgctttg gcttgggaac gcacgtgctc gtgcgaacga aaccacctgc
     1321 gcgacaagca cgttttcacc gtcgcgaaga tgcgcggaga gatcgtacgt ctcgaagaac
     1381 tggtgccgca catcgccctt cgcggggcca cgtccgaccg cgacaccgtt acaccagagg
     1441 atgtaccgcg aatcggcgga gacgtgcaga gtgagacggg catttgccgg tgcagtgaat
     1501 cgacggcgga agtagcccat gagaaacggg ctgtcaccgg tgttggtcgg cgcgctccac
     1561 gagcccatgt catacgcggc gtaatcgatc cagacccacg agctgcggcc attcatagga
     1621 gatgatgcgg tcatacgagt atctcaccga aagaagcgga aagcgtttac aatcctgtgt
     1681 cggaacgata cggcaacgcg cgtcgaagcg acaactgctc cgacgcgcgc gtacctacta
     1741 ttcgtgaggc gcgacagact atgaaat
//
LOCUS       DSCC01000207            1823 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043732, whole genome shotgun sequence.
ACCESSION   DSCC01000207 DSCC01000000
VERSION     DSCC01000207.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043732"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            18..1019
                     /locus_tag="ENN56_02575"
     CDS             18..1019
                     /locus_tag="ENN56_02575"
                     /inference="COORDINATES: protein motif:HMM:PF13646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDS74402.1"
                     /translation="MDFEPPHRGDIVHTHNRSARRGQGILRLNRSLSSTLVGALCIVV
                     VFATHSSGQVASPPGYLRPLLTDLMYGDADVFETAYERVRERSERSLYSDRDLHFVVL
                     ALTQRARLSDELRASIITLLGQTGSPESVPFLIEWVRTDSYEIRLAALQALGWIGDPE
                     AVTAIRRLDIAKADEHTVFMRDYALKAITVKERLRGLPRARNPRPDAWYEIVSETLLE
                     EPNWLVRADIARLLEDWNDRRVWNLVLDMHSIWSGSEPNDSHVRRVLVKRYRYDSRGF
                     LSALRTRDSAAKVFGLDAIADFASPDDLPDFIEIAETDSDPFVREHAARVLGNLLNR"
     gene            complement(1025..1750)
                     /locus_tag="ENN56_02580"
     CDS             complement(1025..1750)
                     /locus_tag="ENN56_02580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:PF01209.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HDS74403.1"
                     /translation="MLKPYRATPEDREHHRRVTTMFNRIAGRYDSLNRIMSGGLDVLW
                     RRRAVRSIDPRVLPRIIDLCTGTGDIALALTKRGHRVVAVDAARDMLLRAKKRPNADA
                     VQWVESDAVELPFANGSFDAVTISFGIRNIADRDGALREFSRVVRPNGRIVVLEALSP
                     TSRFWGTAMRFYESLAFPVLGWLFADDRRAYEYLARSIAGFGDADEFTTDLRRTGWTD
                     PVVTRFVGGSVGLFVATRVTDTV"
BASE COUNT          399 a          483 c          546 g          395 t
ORIGIN      
        1 ccaaacggat tgcgcggatg gatttcgaac cgcctcatcg aggtgatata gtgcatactc
       61 ataatcgatc cgcacggcgc gggcaaggta tcctgcgctt gaaccgaagt ctatcgagta
      121 cacttgtcgg tgcgttgtgt attgttgtgg tattcgcaac gcattcgtcg ggccaagtcg
      181 catctccgcc cggatatttg cgaccattgc tcaccgatct catgtacggc gacgcggacg
      241 ttttcgaaac ggcatacgaa cgagtccgcg agcgttccga acggagtctc tattcggacc
      301 gcgatctgca ttttgttgtg ctcgcgctga cgcagcgtgc acgtttgtca gatgagttgc
      361 gtgcatcgat catcacactg ctcggacaga ccggctctcc agaaagcgtg ccgttcctca
      421 tcgaatgggt gcgcactgat tcatatgaaa tacggttggc ggcgttgcag gcgctcggtt
      481 ggatcggtga tccggaagcg gtcacggcga ttcgacgttt ggatatcgca aaagctgatg
      541 agcacaccgt gttcatgcgc gattacgcgt tgaaggcgat tacggtgaag gaacgtcttc
      601 gcgggcttcc gcgggcacga aatccacgac cggatgcgtg gtacgaaatc gtcagcgaaa
      661 cgttacttga agaaccgaat tggctcgttc gagcagatat tgcgcggctt ttggaagact
      721 ggaacgatag acgagtgtgg aatctggtgc tcgatatgca cagcatttgg agcggatcgg
      781 aaccgaacga ctcacacgta cgacgcgtgc tggtaaaacg ctaccgatat gattctcgcg
      841 gtttcctatc ggcgttgcga acacgggatt cggcggcgaa ggtctttggt ttggacgcga
      901 tcgcggattt cgcgtcacca gacgaccttc cggatttcat cgaaatcgcc gaaaccgatt
      961 ccgatccgtt tgtgcgagag cacgcggcgc gtgtgcttgg taatctgctc aatcgataag
     1021 ggttttacac ggtgtcggtt acccgcgtcg cgacgaacaa tccgacactg ccgccgacaa
     1081 aacgtgtaac caccgggtcg gtccaaccgg ttcggcgaag gtcggttgta aactcgtcag
     1141 cgtcaccaaa accggcgata ctgcgcgcga ggtactcgta agcacgacgg tcatcggcga
     1201 aaagccatcc gagaacggga aacgcgagag actcgtagaa gcgcatcgcc gtgccccaga
     1261 aacgggacgt cggtgagagc gcttcaagca cgacgatacg cccgttcgga cgcacgacgc
     1321 gagagaattc gcgaagcgcc ccatcgcgat ccgcgatatt gcgaattcca aaggaaatcg
     1381 taacagcatc gaacgaaccg ttggcaaacg gaagctcaac ggcgtcgctt tcgacccatt
     1441 gtacagcatc cgcgttcgga cgttttttcg cgcgtagaag catgtcgcgt gcggcgtcca
     1501 cggctacaac gcggtgccca cgtttcgtga gcgcaagcgc gatatcgccc gtgccggtgc
     1561 ataggtcgat gattcgggga agcacgcgag gatcaattga gcgtactgct ctgcggcgcc
     1621 acagaacgtc gagtccgccg gacattatgc ggtttagcga gtcgtaccga ccggcgatgc
     1681 ggttgaacat tgtcgtaacg cgacggtgat gttctcggtc ctcaggggtt gcgcgatacg
     1741 gtttgagaat aacggcggca cctcagcttt ctctcgtttc gattgggttt tatggcgcaa
     1801 gtctgagtat cattcaaagg tag
//
LOCUS       DSCC01000208            2130 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1035321, whole genome shotgun sequence.
ACCESSION   DSCC01000208 DSCC01000000
VERSION     DSCC01000208.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1035321"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1167
                     /locus_tag="ENN56_02585"
     CDS             <1..1167
                     /locus_tag="ENN56_02585"
                     /inference="COORDINATES: protein motif:HMM:PF00773.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HDS74404.1"
                     /translation="KLAYEDVEAVIQGEATDGNPAAPFADTLKGLTELSRRLTQRRID
                     AGALDFDLPEVHVRIGEDGHVASIDKRERLLSHRLVEEFMLLANTSVAHELNARGYDL
                     LYRVHDGPDPEKLSEVINLAATLGHPIKTSDERPSVQQIQRLLEAVKGEPIAPVIETH
                     LLRSMAKAMYQPENIGHFGLATDEYAHFTSPIRRYPDLVVHRQTKRMIASKPTVYTRT
                     ELDDLGRLTSESEQIAEQAEREAIKIKQSEYLAEHLGEEFDGTISGVMRSGFFVILND
                     SLAEGYIGVASLPEDEYYHDQERMSLSGKRRRMQFHFGDPITVQVAQVDTDQGRIDFD
                     FITAPRASERRTASQASELPKRDRKRKDQSKSRKRGNIKNAIKHASRNKRKRRR"
     gene            1170..2093
                     /locus_tag="ENN56_02590"
     CDS             1170..2093
                     /locus_tag="ENN56_02590"
                     /inference="COORDINATES: protein motif:HMM:PF01040.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74405.1"
                     /translation="MRGARVAFQRVFDAIFLARPVLLGPVWVVYGAGAVIAGARPGID
                     LLFVSLVVAGVYIHNQIADVDADRANDKLFLLADGLVSRRTAWTLAVTYWIAALAWAA
                     LHGQRFWLYLTAFLLGFAYNAGVPSPWKGRPWLGLLANAAAHATITFMAGYVAAGGDF
                     AEGFVRSMPYTTAVAAVYLGTTIMDHVGDAAVGKQTFAVRYGARTTSRVIGALIVASA
                     VFAGALGDWWMATASVCAIVSLPWLLRNPSRVSAERVVKTAVLALSVVVAIRWPILGV
                     LAAATFFASRIYYCRRFSIRYPSFGPTQTTL"
BASE COUNT          426 a          595 c          678 g          431 t
ORIGIN      
        1 aagttggcgt atgaagacgt tgaggcggta attcaaggcg aagcaaccga cgggaatcct
       61 gctgcgccat ttgccgatac gctcaagggt ctcaccgagc tatcgcggcg actgacacag
      121 cggcgaatcg atgccggcgc gttggatttc gatctgccgg aagtgcacgt gcggataggc
      181 gaagacgggc acgtcgcatc gattgacaag cgcgaacgac ttctgagtca ccgtcttgtc
      241 gaagagttca tgctgctggc gaatacatca gtcgctcacg agctcaatgc gcgcgggtat
      301 gatttactct accgtgttca cgacggtcct gatcccgaaa agctcagtga agtgatcaat
      361 ctcgcggcga cactcggtca tcctatcaag acgagtgatg agcggccgtc ggtgcagcag
      421 attcaacgcc ttctcgaagc ggtcaaaggc gagccgatcg caccggtcat cgaaacgcat
      481 ttgctgcgga gtatggcaaa agcgatgtat cagccggaga acatcggcca cttcggcctc
      541 gcgacggatg agtacgcgca ctttacgtca ccaatccggc gctatcccga tctcgtcgtg
      601 catcgccaaa cgaagcgcat gatcgcgagt aagccgaccg tgtatactcg taccgagttg
      661 gacgatctcg gtcgcctcac gtcggaatcg gagcagatcg ctgaacaggc tgagcgcgag
      721 gcgatcaaga tcaagcagtc cgaatacctc gcggaacacc ttggcgaaga gttcgacggc
      781 acaatctccg gcgtgatgcg gtccggtttc ttcgtcattc tcaacgattc actcgcagag
      841 gggtacatcg gtgtcgcgag cttgccggaa gacgagtatt accacgatca ggagcgcatg
      901 agcctctccg gaaagcggag gcggatgcaa ttccacttcg gcgacccgat aacggtgcag
      961 gtcgcgcagg ttgacaccga tcaagggcgg atcgacttcg atttcatcac cgcaccgagg
     1021 gcaagcgaac gtcggacggc gagtcaggcg tccgaactgc cgaagcgcga tcggaagcgg
     1081 aaggatcagt caaaatcgag gaagcgcggc aatatcaaga atgctatcaa gcacgcgtca
     1141 cgtaacaagc ggaagcgtcg gcggtagcga tgcgcggggc gagggtcgcg tttcaacgcg
     1201 tattcgacgc gatcttcctc gcgcgaccgg tactgctggg tccggtctgg gttgtctacg
     1261 gtgcgggtgc cgtgatcgct ggagcccggc cggggatcga cctgctgttc gtctcactcg
     1321 tcgttgcggg cgtctacatc cacaaccaga tcgccgatgt cgacgccgac cgggcaaacg
     1381 acaagctctt cctccttgcc gacgggttgg taagccgccg cacggcatgg acgcttgcgg
     1441 ttacgtactg gatcgctgcg ctcgcgtggg ctgcgctgca cggtcagcga ttctggttgt
     1501 atctcaccgc atttctcctc ggattcgcgt ataacgcggg cgtaccgtct ccgtggaagg
     1561 gacgtccgtg gcttggactc ctcgcaaatg cggctgcgca cgcgacaata acgtttatgg
     1621 cggggtatgt ggcggcgggc ggcgatttcg ctgagggatt cgtgcggtcg atgccctaca
     1681 cgacggcagt cgccgcagta tatctcggca cgacaatcat ggatcatgtg ggcgacgcgg
     1741 cagtcgggaa gcagacgttc gccgtacgat atggtgcacg tacgacgtcg cgggtgattg
     1801 gcgcgctcat cgtcgcaagt gcggtttttg caggagcact cggcgactgg tggatggcaa
     1861 cggcgagtgt gtgcgcgatc gtgtcgctac cgtggctgct gcgtaaccca tcgcgcgtct
     1921 ctgcggaacg tgtggttaaa accgcagtgc ttgcgctgag tgttgtcgtg gcgatccgct
     1981 ggccgatact cggtgtgctt gcggcggcaa cgtttttcgc gtcgcgaatc tactattgcc
     2041 gccgcttcag tattcggtat ccgagtttcg gtccgacgca gactacgttg tgagccgctc
     2101 gacaaggtag tcgaggtagc cgtccagatc
//
LOCUS       DSCC01000209            2182 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034200, whole genome shotgun sequence.
ACCESSION   DSCC01000209 DSCC01000000
VERSION     DSCC01000209.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034200"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            41..520
                     /locus_tag="ENN56_02595"
     CDS             41..520
                     /locus_tag="ENN56_02595"
                     /inference="COORDINATES: protein motif:HMM:PF02579.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74406.1"
                     /translation="MRDTRTGRYALNADNMGIRGIYLSSGTKIDSKPCMRIALPKWQD
                     RVSPLFDVAMTVMFVDVDGLREIRRREMMFSTETPVARALRVAAERCDVFICGAISPS
                     LEAVLRASGIRVITDICGPVNEVLASFLQGNLAGEHAYLMPGSGNRTRVRRRWRGGR"
     gene            544..771
                     /locus_tag="ENN56_02600"
     CDS             544..771
                     /locus_tag="ENN56_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74407.1"
                     /translation="MSVSVVQRLALSDRSRAVLQDVVESLFTPSRTESGSKSWAPIIG
                     GIAVTLLSIWLRRQSNQRVEGRRRGRRASNG"
     gene            833..1207
                     /locus_tag="ENN56_02605"
     CDS             833..1207
                     /locus_tag="ENN56_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74408.1"
                     /translation="MPGGDRTGPLGSGPMTGRAAGYCAGYGVPGYANPVGGRGWGGGF
                     GRGGGFGRRNMFYATRLTGWQRAGVGVPTYNVPVAGTVPAAAPISREQQLAMLKDQAA
                     GFADALAAIQKQIDEIEEREDA"
     gene            complement(1309..1382)
                     /locus_tag="ENN56_02610"
     tRNA            complement(1309..1382)
                     /locus_tag="ENN56_02610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1348..1350),aa:Gln,seq:ctg)
     gene            complement(1469..>2182)
                     /locus_tag="ENN56_02615"
     CDS             complement(1469..>2182)
                     /locus_tag="ENN56_02615"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74409.1"
                     /translation="DMEGIRKSLIDGRNGDKFLVGTHLFLFETLRHALGDVTMLESFV
                     LEPAWIRDIGRVLTDFYISHWKMLFTEIGKPDGMFIYEDLGFRSGLFASPKMLGELIF
                     PYYAELVDFYHSEDLPVILHSCGGITEAVPQLIDIGFDCIQPLEAKAGCDVVRLAEEY
                     ADRIAFMGNIDVTVLNTNDRTKVKAHVLGLLERTWDLGADYIFHSDHSVPPDIHFDTY
                     RYAVDPFRDFCATHTRNGG"
BASE COUNT          482 a          555 c          667 g          478 t
ORIGIN      
        1 caagtaatac gttgaattac tattgcagaa cgcagtggta gtgcgcgaca caagaacggg
       61 acgctacgcg ctaaatgccg ataatatggg tattcgtggc atctatctgt cgagtggcac
      121 gaagattgat agtaaaccat gcatgcggat cgcactaccg aaatggcagg atcgggtatc
      181 gcccctgttt gacgtcgcaa tgacggtcat gttcgtcgat gtcgacgggt tgcgcgaaat
      241 ccgacgtcgc gagatgatgt tttcaaccga gacgcccgtt gcgcgtgcgc ttcgtgtcgc
      301 ggccgagcga tgtgacgtat tcatttgcgg tgcaatttca ccgtcgttgg aagcggtgtt
      361 gcgtgcgtcg ggtattcggg tgatcaccga catctgcggt ccggtaaacg aggtgcttgc
      421 atcatttctg caagggaatc tcgccggtga acatgcgtat ctgatgccgg gtagtggcaa
      481 ccggacacgt gttcgcaggc ggtggcgcgg tggtcgatag gacatgagcg ggaggtgata
      541 aatatgtcag tgagtgttgt gcagcgtctc gctctgtctg accgatcgcg cgcggtgctg
      601 caagacgtcg tcgaatcgtt gttcactccg agtcgcacgg agtccggttc gaagtcgtgg
      661 gcgccgatca tcggtggtat cgcggtcaca ctgctctcga tttggcttcg acgacagtcg
      721 aatcagcgcg tcgaaggacg gcgaagaggt cgaagagcat cgaacggatg attcgaggag
      781 tttcctcgac ggattactaa cgagtctttg attgggaaaa aggaggcaca tcatgccagg
      841 tggagatcgc acaggaccgc tcggatctgg tccaatgaca ggccgtgctg cgggttattg
      901 cgcaggatac ggtgttcccg gatacgccaa tcccgtcggc ggacgtggat ggggcggtgg
      961 attcgggcgc ggtggcggtt ttggtcgccg gaacatgttc tacgcgacca ggcttacggg
     1021 gtggcagcgc gccggagtag gcgtacccac gtacaatgtt ccggtcgcgg gtaccgttcc
     1081 cgcggcggcg ccgatttcgc gcgaacagca actcgcgatg ctgaaggacc aagctgcggg
     1141 atttgccgat gcactcgcag cgatccagaa acagatcgac gaaatcgaag aacgcgaaga
     1201 cgcgtgattt cgtaaaggcg atcgagaatc gtttttcgtc gaatgaatgc gtcggaaaca
     1261 aaatgggcgg cttgaaggta actccaagcc gcccattcgc gtgtaccgtg gttgcccccc
     1321 agggattcga accccgataa cgtgatccag agtcacgtgt gttgccatta caccaggggg
     1381 caacgcagac ttttagtata ccgatgtcct caataatcgt caagccgcac gtgccaacgt
     1441 attcccgatt cctcactcct gccgtcggtt agccgccatt tcgcgtatgt gtcgcgcaga
     1501 aatctcgaaa ggggtccacg gcgtaccgat acgtatcgaa gtggatgtcc ggcggaacgg
     1561 aatggtctga gtggaagatg tagtcggcgc cgagatccca tgtgcgttcc aagagcccga
     1621 gaacatgcgc tttgactttc gtacggtcgt ttgtattcag gaccgtcaca tcgatgtttc
     1681 ccatgaaggc gatccggtcc gcgtactcct cggcgaggcg aacgacgtcg cacccggcct
     1741 tcgcttcgag tggttggata cagtcgaagc cgatatcgat cagttgtggc accgcttcag
     1801 tgatgccgcc gcacgaatgg agaatcaccg gaaggtcttc cgagtggtag aaatcgacaa
     1861 gctcggcata gtatgggaag atcaactcac cgagcatctt cggcgacgcg aataatccgc
     1921 tacggaaacc caaatcctcg tagataaaca tgccgtcggg tttaccgatc tcggtgaaaa
     1981 gcatcttcca atggctgatg taaaaatcgg tcagaacacg accgatatcg cgtatccacg
     2041 ctggttccag aacgaaactt tcgagcatgg tcacgtcgcc gagagcgtga cgcaatgttt
     2101 cgaagaggaa caggtgagtt ccgacgagga atttatcgcc gttccgtccg tcaatgagcg
     2161 atttgcggat tccttccatg tc
//
LOCUS       DSCC01000210            2122 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1035513, whole genome shotgun sequence.
ACCESSION   DSCC01000210 DSCC01000000
VERSION     DSCC01000210.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1035513"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..832)
                     /locus_tag="ENN56_02620"
     CDS             complement(<1..832)
                     /locus_tag="ENN56_02620"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612035.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDS74410.1"
                     /translation="MANLLSQINSPADLKDLSPEQLEVLAEEIRQYIVDVISNSSGHF
                     APSLGVVELTIALHRVYDTPRDRIVWDVGHQAYVHKILTGRRDRFPTIRKKDGLSGYL
                     RRSESEYDAFGAGHASTSISAALGIATARDIIGADYKVAAIIGDGAMTGGMPWEALNN
                     AGYAGTDMLVILNDNRMSISPNVGALPKYLNDMISNETYNRIKDDVWEMLGKMPSVGK
                     ILREHISRLDSAVKSLVVPGIVFEKLGIRYFGPVNGHNLSELIPILKDLRELKGPKLL
                     H"
     gene            complement(863..1216)
                     /gene="xseB"
                     /locus_tag="ENN56_02625"
     CDS             complement(863..1216)
                     /gene="xseB"
                     /locus_tag="ENN56_02625"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDS74411.1"
                     /translation="MRIGGVYVTEGTQGPDQSEQRDGKSLDSFESAVKRLEEIVDRLG
                     DQTLPLDQAMKLFEEGIKVSRTCAQYLNDARVRVEKLIEESPGVFSLEAFDQDENVDS
                     AGSVRAENGDSDDES"
     gene            complement(1649..>2122)
                     /locus_tag="ENN56_02630"
     CDS             complement(1649..>2122)
                     /locus_tag="ENN56_02630"
                     /inference="COORDINATES: protein motif:HMM:PF03129.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDS74412.1"
                     /translation="VYETFIDDLPEFGSIMSGGRYDGLVSVFTGQDVPAVGISVGIDR
                     LLAGLQKIGRVESSATLSEVLVTVFDEETAQKSIGAATELRKHGIRAEIYGDTGKLGK
                     QMKYANRLGIPYSLIIGPDEAKAGRMTLRNMKTGDQHDCGGVDEVAAIVRRVRSV"
BASE COUNT          474 a          641 c          520 g          487 t
ORIGIN      
        1 catgaaggag cttcggaccc ttgagctcgc gtaagtcctt gagaattgga atgagttcag
       61 agagattgtg tccgttgacc ggtccgaaat accggatacc gagcttttca aagacgatgc
      121 ccggcacaac caaggacttc accgctgaat cgaggcgact gatgtgctcg cggaggattt
      181 taccgacgga cggcatcttc ccgagcatct cccagacgtc atcctttatc cggttatatg
      241 tctcgttgct gatcatgtcg ttgaggtact tcgggagtgc gccaacattc ggactgatag
      301 acatccgatt gtcgttgagg atgacgagca tgtccgtacc ggcatagccc gcgttgttta
      361 acgcctccca cggcattcca ccggtcatcg caccgtcacc gatgatcgcc gcaactttgt
      421 aatcggcacc aatgatgtca cgagccgtcg cgatgccaag cgctgccgag atactcgtcg
      481 acgcgtgccc tgcaccgaac gcgtcgtatt cgctctcaga gcgccgcaga tatccgctta
      541 gcccgtcctt tttccgaatt gtcggaaatc gatctcgccg gccggtgagg atcttatgca
      601 catacgcttg atgcccaaca tcccacacga tacgatctcg tggcgtatcg tacacgcggt
      661 gcagcgcaat cgtcagttcg acgacaccga ggctcggagc gaagtggccg gagctgttcg
      721 atatgacatc gacaatgtac tgacggattt cctccgcaag tacctcgagt tgctccggag
      781 aaaggtcttt gagatctgca ggactgttta tctgagaaag caggtttgcc atagagtggg
      841 tatcccgtcg gttgacgata cgttacgatt catcatccga atcgccgttt tcggcgcgaa
      901 ctgagccggc gctgtccacg ttttcgtcct gatcgaacgc ttcgaggcta aatacgcccg
      961 gcgactcctc aatgagcttt tcgacacgga ctcgtgcgtc gttcagatat tgtgcgcatg
     1021 tgcgtgagac cttgatccct tcctcgaaaa gcttcattgc ctgatcgagt ggaagagtct
     1081 ggtcgccgag cctatcgaca atttcttcaa gccgttttac cgccgactcg aaactgtcaa
     1141 gagacttccc gtcccgctgc tccgattgat ccggtccctg agttccctca gtcacgtata
     1201 cgcccccaat ccgaatgtgg caacaagccg tagtacgcgc tctacgtcat gcacacgatg
     1261 caaccttaaa tgatacttcc gacaacacga tatgtcaccc cgctgatcga atatacccgt
     1321 tcaaccacca acagaagacc atctcgaagc atcgggttca ccgccgcacg agatcggcgt
     1381 ctacacgaat cgacacacgg ggatacccac atccgtttcg atcgcacgtc aacgagcgtg
     1441 acgacggtca cagacgcagg gtttttcagg ggtatactta ggtcggatgg cgagaataac
     1501 cgcatatccg aacgcggccg ggcgatcaac gtgtgatgct caaagccccc ccaagcagac
     1561 gcccattgca caaggtagct cggtcgcaat atagtaggcg ggatgtcgat gttccatgca
     1621 tcggcatccc gtttcgtttc aatacctgtt acactgatcg gacccgtctg acgatcgcag
     1681 caacttcatc gacgccgcca caatcatgct gatcgcccgt tttcatattc cgcagtgtca
     1741 tcctgcccgc ttttgcttcg tccggaccga tgatcagtga atacgggatt ccgaggcgat
     1801 tcgcgtactt catttgtttc cccaacttgc ccgtatcgcc gtaaatctct gcgcggatac
     1861 catgtttcct cagttcagtc gccgcgccga tcgacttttg cgcagtttcc tcatcaaaaa
     1921 ccgttaccag cacctcgctc agtgtcgcgg agctttccac gcgaccgatc ttctgcaatc
     1981 cggcaagcaa acgatcgata cccaccgata tcccgacagc cggaacgtcc tggccggtaa
     2041 acaccgaaac aagaccatca taccgaccac ccgacatgat gctcccgaat tccggcagat
     2101 catcgatgaa cgtctcatac ac
//
LOCUS       DSCC01000211            1817 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043910, whole genome shotgun sequence.
ACCESSION   DSCC01000211 DSCC01000000
VERSION     DSCC01000211.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043910"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..672
                     /locus_tag="ENN56_02635"
     CDS             <1..672
                     /locus_tag="ENN56_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS74413.1"
                     /translation="VIPVYNERDLVLDVLKRVEEAPTVGLEKEIIVVDDGSTDGTREV
                     LDTVRDRYTVVFHDVNQGKGAALRTGFARATGDIVLIQDADLEYDPNDYPVLLGPFMN
                     DHADVVYGSRFVGGQTHRVLYFWHTIGNRFLTLMSNMFTDLNLTDMEVCYKVFRREVI
                     QSIEIEESRFGFEPEITAKLAKIPGIRMYEVGVSYYGRTYEEGKKIGIKDGLRAIWCI
                     LKYSR"
     gene            complement(714..1538)
                     /locus_tag="ENN56_02640"
     CDS             complement(714..1538)
                     /locus_tag="ENN56_02640"
                     /inference="COORDINATES: protein motif:HMM:PF01522.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74414.1"
                     /translation="MTTYIAAYDTESPSCLAACEKIVEMHHKHEMPATFFITGTTLES
                     NPARYRQILDDPLFEVASHTWSHRMLLDHPMCGPSGTPDEVREQVMRGKALVEDVFGR
                     SCRGLRPGCSFVDGLRGAPEVLELVREAGYEYVSSMAWGADYSLPAPLNQPFTYTEDG
                     FPEIWEIPCHGWHENLLKDNNRWGPRRLTLWPQPWPEAIPADFLKTADDEFAVNRVVL
                     DRARKDNLSFVSLIWHPWSLDRFNPDMEMLDLTFSYVKNVGLQPTTYAGFFDTLRA"
BASE COUNT          432 a          488 c          470 g          427 t
ORIGIN      
        1 gtcatccccg tctataacga gcgcgacctc gtactcgacg tattgaagcg cgtcgaagaa
       61 gcgcctacgg ttggactcga aaaggaaatc atcgtcgttg acgatggttc aacagacggc
      121 acacgagaag tgctcgacac ggttcgcgat cggtataccg tcgtgttcca cgacgtgaat
      181 caagggaaag gcgctgctct gcgaaccgga tttgctcgcg ctaccggcga tatcgtactg
      241 attcaagatg ccgatctcga atacgacccg aatgactatc cggttctact cggcccgttc
      301 atgaacgatc acgcagatgt cgtctacgga tcccgattcg taggtgggca gacgcaccgc
      361 gtcttgtatt tttggcatac cattgggaat cgattcctta ccctcatgtc gaatatgttc
      421 accgatctga atctgaccga catggaagtg tgctacaagg tgtttcgacg cgaggtaata
      481 cagagtatag aaatcgaaga aagccgattt ggtttcgaac cggagattac ggcgaagctc
      541 gcaaaaatac cggggattcg gatgtacgaa gtcggcgttt cgtattacgg ccgcacgtac
      601 gaagaaggga agaagatcgg tatcaaagac ggattgcggg caatctggtg catattgaaa
      661 tacagccggt gaaacgactt caccggctgc atcgtaatat cgcgttcgta tctctacgcg
      721 cgaagtgtat cgaaaaatcc cgcgtatgtt gtgggttgta gaccgacgtt cttcacatat
      781 gaaaatgtta ggtcgagcat ttccatatct gggttgaagc ggtcgagcga ccacggatgc
      841 cagataagcg atacgaaaga caggttgtct ttccgtgcgc ggtcaagaac gaccctgttc
      901 accgcgaatt catcatccgc agtcttcaag aaatccgccg gtatcgcttc gggccatggc
      961 tgcggccaaa gcgtcaagcg acgcggtccc caccgattgt tgtccttcag taggttctca
     1021 tgccaaccgt gacagggaat ctcccagatt tccgggaagc cgtcttcggt atacgtaaac
     1081 ggttgattca gtggagcggg aagcgagtaa tccgcacccc aagccatact actcacgtat
     1141 tcgtacccag cctctcggac gagctcgaga acttccggtg caccacgcag tccgtcgacg
     1201 aagctacatc ctggccgcaa tccacgacat gaccggccga acacgtcttc aaccaacgct
     1261 tttccacgca tgacctgttc acgcacttca tccggtgtgc ctgagggacc gcacatcgga
     1321 tgatcgagta gcattctgtg cgaccacgtg tgcgacgcaa cctcgaatag cggatcatcg
     1381 aggatttgac gatatcgcgc aggattcgac tcgagtgtcg ttccagtgat aaaaaacgta
     1441 gccggcattt cgtgtttgtg atgcatttct acgatcttct cacacgctgc gagacaggat
     1501 ggcgactccg tatcatatgc ggcgatatac gttgtcatct tttctcctta tgcaattacg
     1561 aagaacgacg ccaaccgggc gcgttctcgt cttccgtaca aaatgcgcta agcgcccgat
     1621 ttatggcatc tgctacgtta ttacagtcgt tttcggtgta ccactgattc aatccgatac
     1681 ttatgccgcg atcgaacaag tcatccgcca cggggcagtc gcccttctta tcgcgtcttg
     1741 ccgtgcctgg attctccgtt atcgggaaca tccagtggta gatatgccaa tcgggtcgac
     1801 caccttcgcc gcgcata
//
LOCUS       DSCC01000212            3673 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016978, whole genome shotgun sequence.
ACCESSION   DSCC01000212 DSCC01000000
VERSION     DSCC01000212.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016978"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..720)
                     /locus_tag="ENN56_02645"
     CDS             complement(<1..720)
                     /locus_tag="ENN56_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74415.1"
                     /translation="MDLLSSLRRVLRDTESFRAVVDAVNSRTDAITLHGLAGSLNAFV
                     LDALRNGCDRQILVVTATTNTAEHLRDDLETLLGEQSVRYFPDWELIPFEEKSPHVEV
                     TALRLEVMQSLLIGEPVVVVAPVTALLRPTLEPEALAAASRIISVGDVLDLDELAKWL
                     RSLSFEASNQVVEMGQYARRGGIIDIFTFGAEQPFRIEMFDDEVESIRSFDPATQRSV
                     KSVTEIEVLPRREVLAGDSWWG"
     gene            complement(934..1617)
                     /locus_tag="ENN56_02650"
     CDS             complement(934..1617)
                     /locus_tag="ENN56_02650"
                     /inference="COORDINATES: protein motif:HMM:PF04023.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HDS74416.1"
                     /translation="MTKTVNLLHSQFDRDGEEALGIVWSAHEQGNPTIQAICERWPQM
                     RRLIPYMERANLLRRNGDIVEFTDEGRQRAEQLVRRNRLAEVLLNQVLEVSPEGTAYT
                     ACLWEHMLTSEVTDNICTFLGHPRQCPHGKPIPPGECCRKMVREVTPLVQPLTSLRVG
                     GTGKIVFITPRVHKRLDRLAALGIVPGTEISLKQKSPSYVIRCGETEVAIESSVAADI
                     FVRETSPTK"
     gene            complement(1832..>3673)
                     /locus_tag="ENN56_02655"
     CDS             complement(1832..>3673)
                     /locus_tag="ENN56_02655"
                     /inference="COORDINATES: protein motif:HMM:PF02421.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDS74417.1"
                     /translation="VLQVVDMKNLRRALVLTSQLTDLERTGGIALNMADEARERGISV
                     NVSEIEKIVGVKVEPTVAIRKSGVREIAESSITGRPFTFRIRYSSEIESAIEQLEKLL
                     PSDMRGKRGIALMILAGDETVANWLQERVPTNVIEQCEAVAQNARAKYSVSLNSVISM
                     RRLTEIDRFLTTIMSRRKTVESKVYRNLSQWSTHPVKGLLLLAIVLYVTLWFVGLFGA
                     GTAVDFFETVIFGRYVNPASIWVVDHVLPFPHEHLAEESEYTFALPLSLFHEWEFGTS
                     TTMATSTDYRLTAETLTWWQSLFRLIHDFLVGEYGAITMALSYGFAIVLPIVFTFFIL
                     FSVLEDSGYLPRLAVMTNRVFRIMGLNGKAVLPMILGLGCVTMATMTTRILETKKERM
                     ITTLLLALGVPCSAQLGVILAMVASISIVGVIWWGATVTIVILAVGWLAAKLFKGETS
                     DLVLELPPLRTPQISNILVKTLARIEWYLKEVVPLFILGTAILFILDRTNSLGVIQSF
                     AAPVVQNWLGLPARATEAFLLGFLRRDYGAAGLLVLQQQGFLDEIQVIVSLVTITLFV
                     PCIATVFMIAKEQGVKRAIWMVVFIFPFAFLIGGIIRWLVILSHTVA"
BASE COUNT          852 a         1004 c          972 g          845 t
ORIGIN      
        1 accccaccat gagtcgcctg caaggacttc gcgcctcgga agaacttcga tctcagtgac
       61 actcttcacg cttctctgcg tcgccggatc gaaggagcgg atcgactcca cttcgtcgtc
      121 gaacatctca atacggaacg gttgctcggc gccgaaggtg aaaatgtcga tgatacctcc
      181 gcgcctcgca tattgcccca tctcgacaac ctgattcgat gcttcgaagc tcaagcttcg
      241 tagccacttc gccagttcgt cgagatcgag tacatcaccg acggatatga tccgactcgc
      301 tgctgcgaga gcttccggtt cgagtgtagg acggagtagc gcggtcactg gagcgacgac
      361 aaccacaggc tcgccgatca acaacgattg cattacttcg agccggagcg cagtgacttc
      421 gacgtgtggc gatttctcct cgaaggggat caattcccaa tcagggaagt agcgaacgga
      481 ctgctcgcca agcagagtct cgaggtcgtc acgaagatgc tctgccgtat tcgtcgtcgc
      541 ggttacgacg agtatttgtc gatcgcatcc attgcgaagg gcatcgagta caaacgcatt
      601 taggcttccg gcgagaccgt gaagcgttat tgcgtccgtt cggctgttta ccgcgtctac
      661 tacggcgcgg aacgactccg tatcgcgcag tactcggcga agcgatgata agaggtccaa
      721 ctccaactat ggtactcccg tggattgatg tgcttccgac gctttacgtg atcggaaaca
      781 ccaaaaggcc gacgaccgtc gtttggtcct cggctgctgt tcgcttggtt acgcgaatgt
      841 cggcgtcctg atcgacgttt cgcggcatct ttaaagaata cgcaccacgt tgcgggcgga
      901 caactggttc accactcgcg aaacgtttga ttcttatttt gtcggcgacg tttcgcgcac
      961 gaatatgtct gctgctacgc tcgattcaat cgccacttca gtttctccgc accggatcac
     1021 gtaggatggc gatttctgct tcagtgatat ttctgttccg ggtacgatac cgagtgcggc
     1081 gagccggtcc agacgcttat gcactcgcgg cgttatgaat acgattttgc cagtaccgcc
     1141 gacacggagt gatgtcagcg gttgaacaag cggagtgact tcacggacca tttttcgaca
     1201 gcattcaccc ggcgggatcg gctttccgtg cgggcactgc cgcggatggc cgaggaacgt
     1261 acagatattg tcggttactt cgctcgtgag catatgctcc cacaggcacg cagtatacgc
     1321 ggtgccttca gggctcactt cgagaacctg attgagaaga acctcggcaa ggcggttgcg
     1381 ccggacgagc tgttcagcac gttgtcttcc ctcgtcggtg aattcgacga tatcaccgtt
     1441 tcggcggagg agattggccc gttccatgta cggaatcaag cgacgcatct gcggccaccg
     1501 ttcgcagatc gcttgtatgg tcgggtttcc ctgttcatgc gccgaccaga cgataccgag
     1561 ggcttcttcg ccgtctctat cgaattgact atgcaacaga ttcacggtct ttgtcacctc
     1621 tgctcatcca acgtcggaca agctgtacga gttttgattc cggtggcatt tcgtacccgc
     1681 agtttggaca tcgaaccaga ccacagcgcc ctgccattgg acagcccgaa ggacacgact
     1741 gctgatccgg aggaatcgtc gtctgacaaa tcggacacgt tatatcagcc atttatctat
     1801 ccaatcgacc cggtgtaccg ggttttcgca tttacgctac ggtgtgactc aggatcacca
     1861 gccaacgaat gattccgccg atgaggaacg caaatgggaa aataaacacc accatccata
     1921 tcgcacgttt gacaccttgt tctttcgcga tcatgaaaac cgtcgcaata cacgggacaa
     1981 acagcgtgat cgtgaccaga ctcacaataa cctgaatctc gtcgaggaac ccttgctgtt
     2041 ggagtacgag aaggcccgcg gctccgtaat cacgacgaag aaaaccaagg aggaacgctt
     2101 cggtcgcacg tgcgggaagt ccgagccagt tttgtacaac cggagcggca aaagattgaa
     2161 tgacaccaag cgagttcgtc cgatcgagga tgaagagaat cgccgttccg agaatgaaaa
     2221 gcggcacgac ttccttgaga taccactcga tgcgtgcgag tgttttgaca agaatgttcg
     2281 atatttgggg agttctcagc ggtggtagct caagcaccag atcgctggtt tcgcctttga
     2341 agagtttcgc cgcgagccat ccgaccgcca gtatcacgat cgttaccgtt gctccccacc
     2401 aaataacacc gacgatgcta atcgaagcta ccatggcaag tattacaccg agttgtgcgg
     2461 aacatggaac gccgagcgca aggaggagcg tcgtaatcat acgttctttt ttcgtctcaa
     2521 gaatgcgtgt cgtcatcgtt gccatggtta cgcaaccgag tccgaggatc atcggaagca
     2581 cagccttgcc gttcagaccc atgatccgga atactcggtt tgtcattacc gcgagtcgcg
     2641 gaagatatcc cgaatcctcg agcacgctaa agaggatgaa gaacgtgaaa acgatcggca
     2701 gcacaatcgc aaagccgtat gacaacgcca tggtgatcgc gccgtattca ccgacgagaa
     2761 aatcgtggat gagacgaaat agcgattgcc accacgtgag tgtttcagca gtgagccggt
     2821 agtcagttga cgtggccatt gtcgtcgacg tgccaaactc ccattcgtgg aagagagaca
     2881 atgggagcgc aaatgtgtac tccgactcct cggcgaggtg ttcgtgcggg aacggtaaaa
     2941 cgtgatcgac gacccaaatt gatgccggat tgacataacg accgaagatc actgtttcga
     3001 agaagtcgac agcggtgcct gcaccaaata acccgacaaa ccacagagtt acgtagagta
     3061 ctattgcgag aagtaaaaga cccttaaccg gatgcgttga ccactgtgat aggttacggt
     3121 agaccttgct ttcaaccgtt ttgcgccgac tcatgattgt tgtcagaaat cggtcgattt
     3181 ccgtcagtcg gcgcatggat atgacactgt tgaggctaac cgaatacttt gcacgtgcgt
     3241 tctgtgcgac cgcttcgcac tgttctatta cgtttgtagg gacgcgctct tgaagccaat
     3301 ttgccaccgt ttcatcgccg gcaagaatca tgagcgcgat tccacgcttt ccgcgcatgt
     3361 ccgatgggag aagcttttca agctgctcaa ttgccgattc aatctctgat gagtaacgaa
     3421 tccgaaacgt gaacggacga ccagtgatgc tactctccgc tatctcacgc acgccggatt
     3481 tacggatagc aaccgtcggt tcaactttga cgccgacgat tttttctatt tcgctcacgt
     3541 ttacgctgat gccgcgctcg cgcgcctcat ccgccatgtt tagcgcaatc ccacccgtgc
     3601 gttcgagatc agtgagttgc gatgtcaaca cgagtgcccg tcggagattc ttcatgtcga
     3661 cgacttgtag cac
//
LOCUS       DSCC01000213            2442 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029388, whole genome shotgun sequence.
ACCESSION   DSCC01000213 DSCC01000000
VERSION     DSCC01000213.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029388"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            745..1488
                     /locus_tag="ENN56_02660"
     CDS             745..1488
                     /locus_tag="ENN56_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation protein"
                     /protein_id="HDS74418.1"
                     /translation="MYVIVEADKGEDLGRIGMVSERLRSRKQKGGRRVILRIANEREL
                     ERWRQLRPREEQAFQDFCDRVARREMAMKPVDVEYQLDGRKVIFYFTAEHRVDFRELV
                     RELASVYRTRIELRQIGVRDEAKRLGGIGPCGRELCCTMFLNEFAPITSQMARDQNLS
                     LNPSKLSGMCGRLKCCLRFEHDFYVEARDRFPRLGTRVVFEGCDGQINKLDFFTETVT
                     VRCSDGDDTTISLDDYQRARDMRDGRKRQ"
     gene            complement(1574..1843)
                     /locus_tag="ENN56_02665"
     CDS             complement(1574..1843)
                     /locus_tag="ENN56_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDS74419.1"
                     /translation="MAHKKAGGSSRNGRDSNSQRLGVKAYGGELIHAGSIIVRQRGTH
                     FKPGKNTGRGGDDTIFARATGQVVFKDNSFGKGKVVSIEPVEIAE"
     gene            complement(1886..2200)
                     /gene="rplU"
                     /locus_tag="ENN56_02670"
     CDS             complement(1886..2200)
                     /gene="rplU"
                     /locus_tag="ENN56_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDS74420.1"
                     /translation="MHAIVRVAGVQARVEPGDTIRLPKLNVDAGTSTTFDEVLLLSDG
                     EKVTVGTPTVEGAQVTAEVLKHDRGEKLIVFKKKLRKRYRRTNGHRQDYTEVKITVIV
                     TA"
BASE COUNT          524 a          668 c          724 g          526 t
ORIGIN      
        1 gccttactca tcgaacgagg cgcacgtgag gaaaccgccc actcgattgc gcgactcgca
       61 caaggtgatt tctggcaggc aagcgcgtta tcggccggcg atacgaacga actgcgtcgc
      121 gacgctgcga aaatgattgt tatcgcgctg gatccgaagg aacgagtcgc tgacatcatg
      181 ggtgatatca aggccgctac cgacgggatg tcacctacgg agattagcga gtacgtgcgc
      241 tggatgatct ggtggctgcg tgacctgata cttgcggttg agggcctcca accgccgcga
      301 cccgaagtcg aaccggcgct tgattgggca cggaaagtcg gatttgaacg cctgacggag
      361 tggcttgtag aagctgatcg tagctacgaa atgcttgggc gaaacgttac accggaggcg
      421 gtggtggcgg cgctcacgtt gtatccgcgc gaccaacgtc gactcggtgc ggaaccgacg
      481 ttcccgccgc tcgatgtggt tgtttcgcat agatagaagc accggtaaac agccggtggg
      541 agaatcacgt atatggaacg agaaatacga gaacggaccg aagaagaaga acttcgcggt
      601 cttgaagata gagaagagtt tgaaccgaac ggatttgacg agtccgaaga cgacgatacc
      661 gaaagcccgt tggcagaagt catcttcaaa ggtaatcgtc gagcggtgtt tcggaatacg
      721 agccccgtcg aagcgacgtc gggcatgtat gtcatcgttg aggccgataa gggcgaggat
      781 ctcggccgca tcggtatggt gagcgaacga ctccgttcgc gcaagcaaaa aggtgggcgt
      841 agggtcattc ttcgcatcgc caacgaacgc gagcttgaac ggtggcgtca gcttcgcccg
      901 cgtgaggagc aggcgtttca agacttctgc gaccgtgtgg cgcggcgcga gatggcgatg
      961 aagcccgtcg atgttgagta ccaactcgat gggcgcaagg tgattttcta cttcaccgcc
     1021 gaacatcggg tcgacttccg cgaactggtt cgcgaattgg cgtcagtcta tcgcacgcgc
     1081 attgagcttc ggcaaatcgg tgtgcgggac gaagcgaagc gactcggcgg aatcgggccg
     1141 tgcggacgcg aactctgctg caccatgttt ctcaacgaat tcgcgccgat tacctcgcag
     1201 atggcccgcg accagaacct ctcgttgaac ccgtcgaagc tctccggtat gtgcggacgg
     1261 ctgaagtgct gtctgcgctt tgaacacgac ttctacgtcg aagcgcgcga tcgctttccg
     1321 cgacttggta cccgcgttgt gttcgaaggg tgcgacggcc agatcaacaa gctcgacttc
     1381 ttcaccgaga ctgtcactgt gcgctgttca gatggcgacg atacgacgat atcacttgac
     1441 gactaccaac gcgcgcgcga tatgcgggac gggcgcaagc gacaatagac acgactcggt
     1501 ggtacataca acaagccgtc gttctcatgc gagaacgacg gcttgtttca attttcgcgg
     1561 tgtctctacg tggttattcg gcaatttcga ccggttcgat gcttacgacc ttacccttgc
     1621 cgaagctgtt gtccttgaat accacttgtc ccgttgcacg ggcgaaaatc gtgtcatcgc
     1681 caccgcgacc ggtgttcttt cccggcttga agtgtgttcc gcgctgacga acgatgatcg
     1741 aaccggcgtg gatgagctcg ccgccgtacg cctttacacc cagtcgctgg gagtttgagt
     1801 cgcgaccgtt ccgtgaactg ccgcctgcct tcttatgcgc catgataagc cgtcctttac
     1861 aaaaaccgct cgtcgcctcg aataatcagg cagtgacgat aacggtgatt ttgacttcgg
     1921 tgtagtcctg acgatggcca ttcgtgcgac gataccgttt ccggagcttc ttcttgaaga
     1981 cgatgagctt ctcgccgcgg tcatgcttga gaacctcggc agtcacctgt gcgccttcca
     2041 ccgtcggcgt accgacggtt actttctcgc cgtcggaaag gagcaaaacc tcatcgaagg
     2101 tagtgctcgt gccggcgtcg acattcaact tcgggagccg aatcgtatcg cccggttcaa
     2161 cgcgggcctg cactccggca actcgcacga tcgcatgcat tttcgaacct cgttatcaca
     2221 aagcgaacgg attgctgaca agattttaga atgtaccgct gatagcgtat actgtcaagg
     2281 gcgcgaactc gagcgaggct cgcgttcgtt tgggcgagct gcgctcgccg gtaatcgcgt
     2341 tccggctaac atgcacgaaa ccggatcgca cacgtgtgaa ggctctcgta aaacctttgt
     2401 tatcgtggct tcgggaaata ctgtgcctcg atcatcgcgc ac
//
LOCUS       DSCC01000214            2107 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1035871, whole genome shotgun sequence.
ACCESSION   DSCC01000214 DSCC01000000
VERSION     DSCC01000214.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1035871"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1701
                     /locus_tag="ENN56_02675"
     CDS             <1..1701
                     /locus_tag="ENN56_02675"
                     /inference="COORDINATES: protein motif:HMM:PF02913.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDS74421.1"
                     /translation="IHQIPELLRTLYARASEQSVSISGITRIARGTVNMYATVNPRED
                     ESVIRAGHFMEEMVEVAHDLHGLAMALHGVGIRRIDLLEHNRQQSDLEVLRSVRGVFT
                     PTDAFSVPFVSPPAKERRDLRHRTAREPKIAAVRDAISAQLEHATAENESIQIQASSP
                     QVLSQVLRAAGKHRVTTAIHDRPTGMAVDLSLSELKRVVAFDPDSRVVSVDGGMTLSA
                     LRDVVAKHGLWLPISPLIDRGTCVSDYLAWYRADSRSLGFGPMTGRVVELTAVAGRGD
                     IFSSGGLAGMQHTGPRLSELCIGTRHRYAIITGAAFGLLPAPAMRATVQAEFLKATDA
                     DAAVQRWLGRTDESPKCRPSACCILIDAHYDEDASTHRQEIHAFTEFTGIQSGVERQV
                     RWARHIADEAKAIDLHEAVDAESGESWWFARTHYRQWPGDRGDDVLHLVVWTGIAGWA
                     ELARKIRHVVQVHKYDCKLLIDLGAGRIDVLVAGGAVEPSPLVRLLSELVGRSNAMLE
                     VWSSDLAGKWTGPVSPALETVRSDLKVNFDPSGVLPAGWGRKLVWSTPFESRRTSSTS
                     "
     gene            1814..>2107
                     /locus_tag="ENN56_02680"
     CDS             1814..>2107
                     /locus_tag="ENN56_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74422.1"
                     /translation="MRIAILGTVVYDDIITHLGEHRQSYGGITYNVAALGSLVDEDTE
                     LVPIAQIGDDRFDEVVELFQSFGRVSIDGMNRMPDRKTPMVKLRFTSITARTEN"
BASE COUNT          429 a          644 c          619 g          415 t
ORIGIN      
        1 attcatcaga taccggagct cctccgaacg ctctatgccc gtgcttccga acaaagcgtc
       61 tccatttcgg gcataacgcg aattgcgcgc ggcacggtga acatgtacgc aacagtcaat
      121 ccgcgtgaag acgaatcggt catacgcgcc gggcacttta tggaagagat ggtggaggtc
      181 gcgcacgatc tccacggatt ggccatggcg cttcatggtg tcggcattcg gcgaatcgat
      241 ctcctcgaac acaaccgcca acagagcgat ctcgaagtct tgcggagtgt gcgcggagtg
      301 tttacgccga ccgacgcgtt ctcagtaccg ttcgtttcgc cgccggcgaa agaacggagg
      361 gatttacgcc atcgcaccgc gcgcgaaccg aaaatcgccg cggtacgaga tgcgatctcg
      421 gcgcaactgg aacacgccac tgcggaaaac gaatccattc agattcaagc atcttcccca
      481 caggtacttt cgcaggtcct ccgcgccgca ggcaaacatc gcgtgacgac ggcgatacac
      541 gaccgtccca ccggaatggc cgtcgatcta tcactaagcg agctcaaacg cgtcgtcgca
      601 ttcgatcctg atagccgcgt tgtatctgtt gacggtggca tgaccctgag cgcactccgg
      661 gatgtcgtcg ccaaacacgg tctctggttg ccgatcagtc cgctcattga tcggggcacg
      721 tgcgtcagcg actacctcgc gtggtaccgc gccgatagtc ggtcgctcgg tttcggtccg
      781 atgaccggtc gtgtcgtcga gctcacagcc gtcgcgggac gaggtgatat cttctcgtcc
      841 ggtggtctcg ccggaatgca acacaccggc cctcggctct ccgagttgtg tatcggaacg
      901 cgtcatcgct acgcgattat caccggcgcg gcattcggcc ttcttcccgc accggccatg
      961 cgcgcgacgg ttcaggcgga attcctgaaa gcgacggacg ccgatgcagc cgttcagcgg
     1021 tggctcggtc gaacggacga atcaccgaaa tgccgtccgt cggcatgctg catcctcatc
     1081 gacgcgcact acgacgaaga cgcatcgacg caccggcagg aaatacacgc atttaccgag
     1141 tttaccggta ttcaatcagg cgtagagcgg caggttcgtt gggcgcggca tatcgccgac
     1201 gaagccaagg cgatcgatct gcacgaagcg gtcgatgccg agtcgggcga gtcgtggtgg
     1261 ttcgcacgca cccattaccg gcaatggccg ggtgaccgcg gcgacgatgt gttgcacctt
     1321 gtcgtttgga ccggtatcgc cggatgggcg gagctcgcgc ggaagattcg gcatgtcgtg
     1381 caagtacaca aatacgattg taagctcctc atcgatcttg gcgccggacg aatcgacgtg
     1441 ctcgtcgccg gcggcgccgt cgaaccgagt ccactcgtgc gcttattgag cgaactcgta
     1501 ggacgctcga acgcgatgct cgaagtctgg tcgtcggacc tcgcaggaaa gtggaccggt
     1561 cccgtatcgc ctgcattaga aacggtgcgt tcagatctca aggtgaactt cgatccgtcg
     1621 ggcgtattgc cggcgggctg gggtcgaaag ctggtgtggt cgacaccgtt cgagtcacgt
     1681 cggacatcgt cgacctcgta acgtatacga cctaccttca catgccgcgc gccgctacct
     1741 tatactaccg tattccacgc atcactgagc gtacgcgact cgcggcgtat cgtcgcgcaa
     1801 cttcggcata cctatgagaa tcgctatcct tggcacggtc gtttacgacg acatcatcac
     1861 gcatctcggc gagcatcgac agtcgtacgg cgggatcacc tacaatgtcg ccgcgctcgg
     1921 atcacttgtc gacgaagaca cggagctggt tcccatcgcg cagatcggcg acgaccggtt
     1981 cgacgaagtt gtcgagttat tccagagctt cggccgcgta tccatcgacg gcatgaatcg
     2041 catgccggac cgcaaaacgc cgatggtcaa actccgattc acgagtatca ccgcgcggac
     2101 cgagaat
//
LOCUS       DSCC01000215            1584 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052856, whole genome shotgun sequence.
ACCESSION   DSCC01000215 DSCC01000000
VERSION     DSCC01000215.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052856"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            8..814
                     /locus_tag="ENN56_02685"
     CDS             8..814
                     /locus_tag="ENN56_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDS74423.1"
                     /translation="MTYRDVAEQAARVGARILLEHFRELDSAEIQTKQRNDFVSFVDN
                     QSERAIRDVILGAYPHHRFVGEEQGARGSDDATWRWIVDPLDGTSNYVHGVPQFSVSI
                     ALEHAGELIASVVLDPIRNDLYSAELGSGAFLNGERIRVADQQSFEDALIGTGFPFRH
                     HEVLDDYLATFREIFPRVAGVRRPGSAAIDFTWVACGFTAGFWEFLLSPWDVAGGALL
                     ITEAGGVITDFAGGNDFLTSGNVVAGAPHIHRELLSIVQGVLRDRGVRLS"
     gene            845..>1584
                     /locus_tag="ENN56_02690"
     CDS             845..>1584
                     /locus_tag="ENN56_02690"
                     /inference="COORDINATES: protein motif:HMM:PF04616.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74424.1"
                     /translation="MSILYSPDDAFAADFIPLYHNGEYHLFYLKDWRDPEHHGEGTPW
                     WHVVTRDFVTFEDRGEALPRGAKDEQDLYVFTGSAIEAEGRFHIFYTGHNHHLKDSGC
                     PQEAIMHAVSDDLDTWVKDPDMRFFAPSDYEPHDWRDPFVFWNDEVGEYWMLLAARKT
                     AGPDRNRGLVACAASTDLVNWRVRKPVWAPDTYYTHECPDLFRIGDTKYLIWSEFSDR
                     FRTRYRLADSFDGPWRRPSDDQFDDRAY"
BASE COUNT          302 a          456 c          470 g          356 t
ORIGIN      
        1 gaattagatg acgtatagag acgtcgcaga acaggctgcg cgagtcggtg cccgtattct
       61 actcgagcac tttcgcgaac ttgattccgc tgaaatccag acgaagcaac gaaacgactt
      121 cgtctcgttt gtcgacaacc agtccgagcg ggcgattcgc gacgtgattc tcggcgcgta
      181 tccgcatcat cggttcgtcg gcgaggaaca gggcgcacgc ggttctgatg acgcaacatg
      241 gcgctggatc gtcgatccac ttgacggcac gagcaactac gttcacggcg tgccgcagtt
      301 cagcgtatct atcgcgcttg aacacgccgg tgagcttatc gcgtcggtcg tgctcgatcc
      361 gattcggaat gatctctaca gcgcggagct gggttccggt gcgtttctca acggcgagcg
      421 gattcgcgtc gccgaccaac aatcgttcga agatgcgttg atcggtaccg gctttccctt
      481 ccgacaccac gaagtcctcg acgattacct tgcgacgttt cgcgagatat tccctcgtgt
      541 cgcaggagtc cgacgaccag gttctgcagc gatcgatttc acgtgggttg cgtgtggctt
      601 tactgcgggg ttctgggagt ttctcctttc gccgtgggac gtcgctggcg gagcgctttt
      661 gatcactgag gcaggtggtg ttatcaccga ttttgccggc gggaacgatt ttctcacgtc
      721 ggggaacgtc gtcgccggag cgcctcatat ccatcgtgaa ctcctgtcga tcgttcaagg
      781 tgtcctgcgc gaccgcgggg tacggctctc gtagtaaacc gcttctttgt gttggaggtg
      841 tgcaatgtcg attctgtata gccccgatga cgcatttgcc gcggatttca tcccgctgta
      901 tcacaacggc gaataccacc tgttctacct caaagactgg cgcgatccgg agcatcacgg
      961 cgaaggtaca ccgtggtggc acgtggtcac gcgggacttc gtcacgttcg aggatcgcgg
     1021 cgaagcgcta ccccgtggag cgaaagacga gcaggatctc tacgtgttca ccggtagcgc
     1081 aatcgaggcg gaagggcggt ttcacatttt ctacacgggg cataatcacc acctcaaaga
     1141 ttccggatgt ccgcaggaag cgatcatgca tgcggtgagc gatgacctcg acacatgggt
     1201 aaaggacccc gacatgcgct tcttcgcgcc gtcggactac gagccgcacg actggcgtga
     1261 tccgttcgtt ttttggaacg acgaggtcgg tgaatactgg atgctcctcg ccgctcgaaa
     1321 aaccgctgga ccggatcgga atcgtggcct cgtcgcgtgt gccgcgtcaa ccgatctcgt
     1381 gaactggcgt gttcgcaagc cggtttgggc gccggacacg tattacactc acgagtgccc
     1441 cgatcttttc cgaatcggcg atacgaaata cctcatctgg tcggagttct ccgatcgatt
     1501 ccgcacgcgg taccgcctcg ccgactcgtt tgacgggccg tggcgcaggc catccgacga
     1561 ccaattcgac gatcgcgcgt acta
//
LOCUS       DSCC01000216            2249 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032805, whole genome shotgun sequence.
ACCESSION   DSCC01000216 DSCC01000000
VERSION     DSCC01000216.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032805"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..638)
                     /locus_tag="ENN56_02695"
     CDS             complement(<1..638)
                     /locus_tag="ENN56_02695"
                     /inference="COORDINATES: protein motif:HMM:PF01578.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HDS74425.1"
                     /translation="MHVKNWALWLIATILVIVGFVVSVYLVFIGTGIERNLGFSQKIF
                     YFHVPCAWITYLAFGIAAVSSVIFLIRSNPIWDSIAYASIELGTVFTTLVIVTGPLWA
                     RPTWGAYWVWDPRLTTTLILWLIELSYLLLRAFVDEPEKAAKYGAILALLGALDIPII
                     HYSVVLWRGMHPAVIRSEAGSGGLPHEFFVALMVSLATFTLLFFMLLIVRVRL"
     gene            complement(697..1365)
                     /locus_tag="ENN56_02700"
     CDS             complement(697..1365)
                     /locus_tag="ENN56_02700"
                     /inference="COORDINATES: protein motif:HMM:PF03379.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDS74426.1"
                     /translation="MRVFWAILWKDVRQEWRRRELVLVMAVFSIVVLVLFNFALGLSP
                     DRPQNVAAAVMWIAFIFAGTLGLNRSLAMERDDQCLRALLLSPADRSWIYLAKAVGNM
                     VFLLVVEIMVVPLTLVLFNLNVPSLTGWLALTCILGTTGFVGVGTLLAGMATGTRMRD
                     WLLPLLLFPIQVPVLISAVQATQLLLNNRPLDEITLWLKLLVAFDLIFVAASVLAFEY
                     IVEE"
     gene            complement(1365..2111)
                     /locus_tag="ENN56_02705"
     CDS             complement(1365..2111)
                     /locus_tag="ENN56_02705"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS74427.1"
                     /translation="MLTAKESDRSTPSGIRVVGLTKWYGAVPALRGADIDVPSGTCLA
                     LYGPNGAGKSTLIGILSTLVRPTEGTAEIAGFDVRREGTEVRRRLGVISHHPWVYDRL
                     SVRDNLCFFAKLYGIENASDRIDSLLRDVELDRWIDQDAGTLSRGMRQRLTIARALLH
                     DPPVLLLDEPFTGLDRHSAKVFAGRLSALRAQGRTILLVTHHLEEGWTLADKAAVLVR
                     GRIAHSADVTPDGMDHFAATFDRLLEGNGS"
BASE COUNT          575 a          633 c          589 g          452 t
ORIGIN      
        1 agtcgaacgc ggacgatgag gagcatgaaa aagagtaacg tgaatgtggc gagtgacacc
       61 atcaatgcaa cgaaaaactc gtgcgggagt cctccgcttc cggcctctga ccgtatcact
      121 gccggatgca tcccgcgcca tagaacgact gagtaatgaa tgatcggaat atccaaggca
      181 cctaacaaag caaggatagc cccgtatttc gctgcctttt ccggctcgtc aacgaacgct
      241 cgaagcaaga gatacgatag ttcgatgagc cagagtatca acgttgtagt aagtcgtgga
      301 tcccacaccc agtatgcgcc ccaggttggg cgcgcccaca ggggtcccgt tacgatcaca
      361 agtgtcgtaa acacggtgcc cagttcaatg ctcgcgtacg cgatgctatc ccatatcggg
      421 ttgctacgga tgagaaagat gacacttgat accgcggcaa taccgaacgc gagatacgtg
      481 atccacgcgc agggaacatg gaaatagaag atcttctgcg aaaacccaag atttcgctct
      541 attccggtgc cgatgaatac cagatacacc gagacgacaa aaccgacgat cacgagaatc
      601 gttgcgataa gccaaagagc ccagtttttc acgtgcatac cacgcttccc acattccgct
      661 tttccgcacc gagaaggcgc gtcagcggta tagacgttat tcctctacaa tatattcgaa
      721 cgccaatacc gatgcggcga cgaatatcaa atcgaacgcg accagtagtt tcaaccagag
      781 cgtgatttcg tccagcggac gattgttgag gagtagctgc gttgcttgaa cggcgctaat
      841 gagaaccggc acctgaatgg gaaacaggag taacggaagt agccagtctc gcatacgtgt
      901 cccggtagcc attccggcga ggagcgttcc cactccaacg aaacctgtcg tacccaatat
      961 acacgtaaga gccagccaac cagtcaacga cggtacgttc aggttaaaca acacaagcgt
     1021 caagggaacg accatgattt cgacaacaag gagaaacacc atattgccga ctgcttttgc
     1081 gagatagatc catgaccggt cggcaggact caggagaagt gcgcgaaggc attggtcgtc
     1141 gcgttccatc gcgagcgaac ggttcaatcc gagagtaccc gcaaaaatga acgcaatcca
     1201 catgactgca gcggcaacgt tctgtggtcg atccggtgag agaccaagtg cgaaattgaa
     1261 cagcacgagc acaacgatgg agaaaacggc catgacgagg acgagctcgc gtcgccgcca
     1321 ctcctgccgc acatccttcc agagtatcgc ccagaagacg cgcattagga tccgtttcct
     1381 tccagcaacc gatcaaacgt tgcggcgaaa tgatccatcc cgtccggcgt tacgtcagcc
     1441 gagtgcgcga tccgtcctcg caccagtact gcggctttat ctgcgagcgt ccacccttcc
     1501 tcgagatggt gcgttacgag caagattgtt cgaccttgtg cacgtaacgc cgaaagacgc
     1561 ccggcgaaaa ccttagccga atggcgatcg aggccggtaa atggctcatc gagcaacaat
     1621 accggtggat catgcaggag cgcgcgcgcg atcgtcaaac gttgtcgcat tccgcgtgat
     1681 agcgtgcccg cgtcttggtc aatccatcga tcgagttcga cgtcgcgaag caacgaatct
     1741 atgcggtccg acgcattctc gataccgtac agttttgcga aaaaacaaag gttgtcgcga
     1801 acgcttaggc gatcgtatac ccacgggtga tgcgatatta cgccgaggcg ccgtcgaacc
     1861 tctgttcctt cgcggcggac gtcaaatccc gcaatttcgg cggttccttc ggttgggcgc
     1921 acgagcgttg acagtatgcc gatcaacgtc gatttcccgg ctccattcgg tccatacaga
     1981 gcgagacagg tgccgcttgg gacgtcgatg tcggccccgc gtaacgcggg tacggctccg
     2041 taccactttg tcaaaccgac aacgcgtata ccgctcggtg tcgatctgtc gctttcttta
     2101 gccgtcaaca atcagaatcg ccatcgcaaa gacatgtcgg agagctgcca cgtttcgcac
     2161 attcacaaca gagtagcgtt ttaccgttcc tcatttttta gcgcgcgaat gagtcgcgct
     2221 ttcaagtctc gacgctgctg aacatattc
//
LOCUS       DSCC01000217            3417 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018758, whole genome shotgun sequence.
ACCESSION   DSCC01000217 DSCC01000000
VERSION     DSCC01000217.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018758"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..471
                     /locus_tag="ENN56_02710"
     CDS             <1..471
                     /locus_tag="ENN56_02710"
                     /inference="COORDINATES: protein motif:HMM:PF02954.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS74428.1"
                     /translation="DLFYRLNVVEIRLPPLRERPGDIMILADHFRQRFCARDQKRITG
                     FTDRARDALESYRWPGNVRELENAIERAVVLTTGDTIDGKHLPKEVLSDEESGSEIVI
                     PIGTTLDDAKCRIIRATLHAADGDKKRAAEMLGIATRTVYRTLERFCAGGGNEE"
     gene            complement(478..2199)
                     /locus_tag="ENN56_02715"
     CDS             complement(478..2199)
                     /locus_tag="ENN56_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74429.1"
                     /translation="MSRMLHLLALFAALLACAGSLRAVEEQSADSTFVIGGILTQVGD
                     VIPPWSEEAHDSNRILQFGYRLTNALHIATRKHVVERELLFTVGDTLDTLSLQETARN
                     LRAYPFINEAEVTPYTDESGATIVHVRTSDNFSIAPGFIFEGGGGGTTYGAILTETNL
                     FGLGKRVAGQYIRETGTVNTTTWAFEYKDHRVFGSRWFFHLYAEDLATGSQIATVMEY
                     PFYRLSSQNASGWSLDFNDSYRRMYGTTSSRAGDIVAEIPRRYIGGNAWYSYRWGDHY
                     HRTKLTSAIAWYDYSYSGLPRTIVDTHYTITQADTTRTPRRGYQLTASIGREAFTGFL
                     TSRFLDDFLVVEDVDTGWEFGATGGVGIPEKPTHELYGITGVYGSYANVFGASGDHIV
                     AAEATGVVHLADDHGLGRRTWSNLVTDAWVHWYWQGLPIQTIATSLNWYAGWRMYDPF
                     QITLGGDTGLRGYATNQFAGNRRLLLNVEDRIFPGWRIFTVAIGLVGFADAGYVWKAD
                     ERLNPADLHANVGFGLRVGNTRASTSRISRLDFAFALRGEKRIMLAFGSEQVFDLFNI
                     RPTPTRD"
     gene            complement(2196..3296)
                     /gene="lpxB"
                     /locus_tag="ENN56_02720"
     CDS             complement(2196..3296)
                     /gene="lpxB"
                     /locus_tag="ENN56_02720"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDS74430.1"
                     /translation="MRVFLSAGEPSGDRWGAALALALRRSRTDIECIGITGDTMREAG
                     VRDIRPATSLRVDGVMGFAQPFRVYRTLRDTLTAITASWKMERPDVVVAIDYPGFHMR
                     VLARAKERGIRTVYYIPPQVWAWRPKRARDVAAVSDIVVPAFDFERPYYTTWMPSGRV
                     LWMGHPLADSMPKPSGRRNDGHRSIALLPGSRRSEVTRLASRMAGAVQRTDLGEPVFA
                     LHAEEAVDWLPRDVRDLRRVVGDTPKTLETAAAAIVCAGSATLEAACAGLPTIICYRT
                     DALTYGIAKMLVKTPWVGLPNVLLNGAYYPELIQRAATEASIANALRTVMDERLETWE
                     RRAETLRDVCGEPGVADRVATVVLETANGARP"
BASE COUNT          699 a         1053 c         1001 g          664 t
ORIGIN      
        1 gatctattct atcgtctcaa cgtcgttgaa attcgtctac cgccccttcg cgagcgtccc
       61 ggtgacatca tgattctcgc cgaccatttt cggcaacgct tttgcgcgcg cgaccagaag
      121 cgaatcaccg gattcaccga ccgtgcgcgt gacgcgctcg aatcgtatcg ctggccgggg
      181 aatgtgcgtg aactggaaaa cgcaatcgag cgagcggtcg tgctgaccac tggcgataca
      241 atcgatggaa aacacctgcc aaaagaagtg ctcagcgacg aggaatccgg aagcgagatc
      301 gtgattccga tcggcacgac gctggatgac gcgaaatgcc gcatcatccg tgcgacgcta
      361 cacgcagccg acggcgacaa gaagcgcgcc gccgagatgc tcggtatcgc cacgcgcacc
      421 gtgtatcgca cactcgagcg tttctgcgcc ggtggcggga acgaggagta gcggggtcta
      481 atcccgtgtc ggcgtcgggc ggatgttgaa cagatcgaat acctgctcgg agccgaacgc
      541 aagcatgatc cgcttctccc cacgcagcgc gaacgcgaaa tccaaccgcg agattcgcga
      601 agtcgacgcg cgagtgttcc ccacgcgcag cccaaacccc acgttcgcat gcaagtcggc
      661 cggattcaac cgttcgtccg ctttccacac gtatcctgca tcggcgaacc cgacaagacc
      721 gatcgcgacg gtgaatatcc gccatccggg gaatatacgg tcctcgacgt tgagaagaag
      781 tcggcggtta ccggcgaatt gattcgtcgc gtagccgcgc aatccggtat caccgccgag
      841 tgttatctgg aacggatcgt acatgcgcca tccggcgtac cagttcagac tcgtcgcgat
      901 cgtttgtatg ggcaatccct gccaatacca gtgcacccac gcgtcggtga cgaggttcga
      961 ccacgtgcgt cgtcccaaac cgtgatcgtc cgcgagatgc accacgccgg tcgcctccgc
     1021 cgcaacgatg tggtcgcccg acgcgccgaa tacgttcgca tagctcccat acacaccggt
     1081 gattccgtac agctcgtgcg tcggcttttc cggaataccg acgcctcccg ttgcgccgaa
     1141 ctcccagccg gtatccacgt cctcgacgac aaggaagtcg tcaaggaatc gtgaagtgag
     1201 aaagcccgtg aacgcctcgc gcccaatcga agccgtcaat tgataaccgc gccgcggtgt
     1261 acgagtggta tccgcttgtg tgatggtgta gtgggtatcg acgattgtcc gggggagacc
     1321 ggagtaggag tagtcgtacc atgcgatagc gctcgtcagc ttggtccggt ggtagtggtc
     1381 gccccatcgg taggaatacc atgcatttcc cccgatatat cgccgcggta tctccgcgac
     1441 gatgtcaccg gctcgggacg aagtggtgcc atacatgcgt cgatagctgt cgttgaaatc
     1501 caatgaccaa ccggaggcat tttgcgagga gagtcggtaa aacgggtact ccatgactgt
     1561 ggcgatttgc gagccggtcg caagatcttc cgcatagaga tggaaaaacc agcgagaccc
     1621 gaaaacccga tgatccttgt attcgaacgc ccacgttgtc gtattaaccg tgccggtttc
     1681 ccggatgtat tgccccgcga cccgcttccc gagaccgaaa aggttcgtct ccgtgagaat
     1741 cgcaccgtat gttgttccgc caccgccgcc ttcgaagatg aaccccggtg cgatactaaa
     1801 attgtcgctt gttcggacgt ggacgatcgt tgctccggat tcgtcggtat agggtgtcac
     1861 ttcggcttcg ttgatgaacg ggtacgcgcg gagattgcgt gccgtttcct gaagactgag
     1921 cgtgtcaagc gtgtcgccaa ccgtgaaaag tagctcccgt tcgaccacgt gcttccgggt
     1981 agcgatatgt aacgcattcg tgaggcggta gccgaactgc agaatgcggt tgctgtcgtg
     2041 cgcctcctcg gaccacggcg gtatcacgtc accgacttgt gtcagaatac cgccgatgac
     2101 gaaggtcgaa tccgcggact gctcttcaac agcacgcaac gagcctgcac acgcaagcag
     2161 cgcggcgaaa agtgctagca ggtggagcat gcggctcatg gacgcgctcc attggccgtt
     2221 tcgagaacta ccgtggcgac ccggtccgct acgcccggct ctccgcacac gtcgcgcaac
     2281 gtttcggcac gacgttccca tgtttccaat cgttcgtcca tgacggttcg caatgcgttt
     2341 gctatcgagg cttccgttgc ggctcgttga atgagttcgg gataatacgc cccgttgagt
     2401 aacacattcg gcaatcccac ccagggcgtc ttcacgagca ttttcgcaat accgtacgtg
     2461 agcgcatcgg tccggtaaca gatgatcgtc gggagcccgg cgcacgcggc ttcaagcgtt
     2521 gcgcttcccg cacatacaat cgccgcggct gccgtttcca gcgtcttggg cgtgtctcct
     2581 actacgcggc gaagatcacg gacgtcgcgc ggtagccagt cgaccgcttc ttcggcgtgg
     2641 agcgcaaaca ccggctcgcc gagatcggtt cgttgaacgg cgcccgccat ccgcgacgcg
     2701 agtcgcgtta cctcacttcg gcgactgccc ggaagcaatg cgatactgcg atgcccgtcg
     2761 ttgcgacggc cgctcggctt cggcatcgag tctgcgagcg gatgtcccat ccacagcacg
     2821 cgaccggacg gcatccacgt ggtataatac ggccgttcga aatcgaatgc cggtacgacg
     2881 atatccgata ccgctgcgac gtctcgcgca cgcttcggcc gccacgccca cacctgcggc
     2941 ggaatataat atacggtgcg aatgcctcgt tctttcgcac gtgcgagcac acgcatatgg
     3001 aatccggggt aatcgattgc cacaacgaca tccggacgtt ccatcttcca ggaagcggta
     3061 atcgcggtaa gcgtatcgcg gagcgttcgg tagactcgaa acggttgggc aaaccccatg
     3121 acgccgtcga cgcgcagtga ggtggccggc cgaatatctc gaacaccggc ttcacgcatc
     3181 gtatcacccg tgatgccgat acattcgatg tcggttcgtg aacgacggag cgccaaagcg
     3241 agcgccgcgc cccatcggtc gcccgacggt tcacccgccg agaggaagac gcgcatggcg
     3301 ctttcgtcgc tggagtgtac cgcgtcggag tccgagaacg agcgtcgcac cgagaaacgc
     3361 ggcgatggct tgataggtcc gtacttccga ccgaagcgca gccggccagt cagccgc
//
LOCUS       DSCC01000218            1764 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045641, whole genome shotgun sequence.
ACCESSION   DSCC01000218 DSCC01000000
VERSION     DSCC01000218.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045641"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..280
                     /locus_tag="ENN56_02725"
     CDS             <1..280
                     /locus_tag="ENN56_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HDS74431.1"
                     /translation="VSYGLLDVISFFTVGKDEVRAWTIRRGSVAPEAAGTIHSDLERG
                     FIRAEVVTYDDLMRTGSHAAARDAGVLRLEGKSYIVQDGDILNIRFNV"
     gene            302..1657
                     /locus_tag="ENN56_02730"
     CDS             302..1657
                     /locus_tag="ENN56_02730"
                     /inference="COORDINATES: protein motif:HMM:PF00480.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDS74432.1"
                     /translation="MPKRPAVIGIDLGGTKLLAAVIDSDGNVIDAKKVTSPKTNSDAI
                     VERLITVIEALRTQHGENGTEIAAIGLGVPGVVDRESGTVIAAPNLPLEGVELGRIVS
                     GASGLPTEVGNDVTLATMGEAWFGAARGVENVLGIFVGTGIGAGIVVDGKMYLGAHGG
                     SGEIGHVLMQRDGPECGCGRRGCLEALASRTAIERDIRSEVEDGRMSVLGDRVRAGKR
                     LRSGRIAAALEEGDSLTTEVVNRASSILGTAIGSVVNVLDPDLVVVGGGLVEACGLFM
                     MPVIIESANSRMMPVPGGPVPVVPSQLGDNAGILGAAALAFEAHNVRKPGGATAYAPV
                     VEWVADGEVVVNGERRVDDFFIRADGKVRKRKRRLSRAELGTDRALSVAEAQFICDGR
                     PSVLIVGAGERGQLPISNDARSWLESQGIRLVSAPTRAAITEYRVRKGPRALLLYLRD
                     S"
BASE COUNT          351 a          452 c          581 g          380 t
ORIGIN      
        1 cgtatcgtat ggtctactcg atgtgatcag cttcttcacc gtcggaaagg atgaggtgcg
       61 cgcgtggacg attcgccgcg gatcggtcgc tccggaagcg gcgggaacga tacacagcga
      121 cctcgaacgc ggcttcattc gtgctgaagt cgtgacgtac gacgacctca tgcgaaccgg
      181 ttcacatgcc gccgcgcgag atgccggtgt cttgcgtctg gaggggaaga gctatatcgt
      241 gcaagacggg gacattctaa acatacgttt taatgtgtaa cgtactttgg gggatggagt
      301 tatgccgaaa cgcccggcag taatcgggat tgatctcggg ggtacgaaac ttctcgctgc
      361 ggtcatcgac agtgacggaa acgtcatcga cgcgaaaaaa gtaacttcac cgaaaaccaa
      421 cagcgacgca attgtcgagc gactcatcac ggtcatcgaa gcactccgta ctcaacatgg
      481 cgagaatggc acagagatcg ccgctatcgg acttggtgtt ccaggagtgg tcgaccggga
      541 atcaggcacc gtaatcgccg cgccgaattt gccgcttgaa ggtgttgaac ttggtcgtat
      601 cgtgtctgga gcatctggac tgccgacgga agtgggcaat gacgttacac tcgcgaccat
      661 gggtgaagcg tggttcggtg ccgcgcgagg cgtcgagaat gttctcggta tcttcgttgg
      721 aaccggaatc ggtgcaggca tcgttgtcga tgggaagatg tacctcggcg cacacggcgg
      781 atccggcgaa atcggtcacg tactcatgca acgtgacggt ccggagtgcg gatgcggtcg
      841 ccgtggatgc ctcgaggcac tggcgagtcg tactgcaatc gagagagata ttcgctccga
      901 ggtcgaagac ggtcgtatgt cggtacttgg cgatcgtgtt cgtgctggga aacggttgcg
      961 tagcgggaga atcgccgcgg cgcttgagga aggcgactcg ctgacaacgg aagtcgtcaa
     1021 tcgtgcctcg tcgatcttgg gtacggcgat tggcagcgtc gtgaatgtgc tggatccgga
     1081 tctcgttgtt gttggtggcg gactcgtcga agcgtgcggg ttgtttatga tgcccgttat
     1141 tattgaatcg gcgaattcgc gcatgatgcc ggttcccgga ggtccggtac ccgtcgtgcc
     1201 atcacagctt ggtgataacg cggggatcct tggcgcggcc gcgctggcct tcgaggcgca
     1261 caacgtacgc aagcccggag gtgcgacggc atatgcgccc gttgtcgaat gggtggccga
     1321 cggggaagtc gtcgtgaacg gcgagcgtcg agtggacgat ttcttcattc gtgcggacgg
     1381 caaggtacgg aaacgtaagc gtcgactttc gcgcgcggaa cttggaacag atcgagcgtt
     1441 gagcgttgcg gaggcgcagt ttatctgtga cggacgccca agcgtactga tcgttggcgc
     1501 cggtgagcgt ggtcagttac ccatatcaaa cgacgcgcga agttggcttg agtcacaggg
     1561 aatccgtctc gtcagcgcgc cgacacgtgc cgcgatcacc gaatatcgcg tgcggaaagg
     1621 acctcgcgcg cttttgttgt atctgcgcga ttcgtgaggt ttcccatggt cggtgcgtcg
     1681 atactttcgc ttcgcggacg tcacggtggt gataccgacc acatggacca cgtctgccgc
     1741 ctcgcgacga ccatatttct ccag
//
LOCUS       DSCC01000219            2615 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026821, whole genome shotgun sequence.
ACCESSION   DSCC01000219 DSCC01000000
VERSION     DSCC01000219.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026821"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(635..1300)
                     /locus_tag="ENN56_02735"
     CDS             complement(635..1300)
                     /locus_tag="ENN56_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDS74433.1"
                     /translation="MGNLSLDNLPYILLTVLPIGLMAVTLHEVSHGWVAKRFGDPTAD
                     MEGRLSLNPIKHIDPIGALMIVIIGFGWAKPVPVNPAYFKRPGRDMMWVGLAGPISNI
                     LFAIVFAILLRITVYVYYSTEILPLEATMAVIRFFQFGIMINVILAAFNMIPIPPLDG
                     SRVLAYFLPRDLEMRYRDLERYGMFILIGVIFVLPWVTNGRIDVIRIVAGGALRVFQT
                     IFL"
     gene            1434..2384
                     /locus_tag="ENN56_02740"
     CDS             1434..2384
                     /locus_tag="ENN56_02740"
                     /inference="COORDINATES: protein motif:HMM:PF03547.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDS74434.1"
                     /translation="MFRTPYRETLSTLARRSALPFLTIILPIFLIFGLGYLLGRFRHA
                     ESKALVDMALYLFTPALIFSRLVANPVSLVDAGKIGVYSWALCALMYVAGWIVARAIG
                     LDRLDRYALLLGVVTMNAANYGLPVVEFAVGPHAVAYAAVFILAVNIVQSTVGVYLAA
                     AGRRSPWESFLSVFKLPLVYAFVAAFVIRGLDITLPEPIFRPMMLLGDAAIPVAMVIL
                     GIQLTKVHLGGAWKNLTAITAIRLILSPLVGLALAWALGLHGDMRFALILEAGMPTAV
                     NAGLLAAEFDTKPEFVTGAIMVTTLASAVTLSVIISLFGG"
     gene            complement(2390..2596)
                     /locus_tag="ENN56_02745"
     CDS             complement(2390..2596)
                     /locus_tag="ENN56_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74435.1"
                     /translation="MLDIERFRYQLRRRSMSRAEVPLGMKHDLRDAVPDLHEDPDRIS
                     PRFIEPTMRWIREVIPLPETVRDR"
BASE COUNT          548 a          748 c          746 g          573 t
ORIGIN      
        1 acggtatcaa acggatgaat agccggatcg gtgaaaagtc gcgtgatgcg gaaatcgccg
       61 tgcgtcgcgt ttgcgccgtg cgttgctttc gcgtcgcgcg cagcgttcgc ttcgtgcgtt
      121 gcggtcgttt ccgcctgcac gtcgttgact ggagccgaca tcattgcccc cttgggagta
      181 aaccggtgta caaaaaagag aaacgagggc gtatacactc catatagatg tgtcatacaa
      241 cacgttacgc tatatgcagt gtattcgccc ggcgtcagat cgtcttcagg tgtggtgata
      301 gaaggatata ccacaagcca tagtgtggtc aagttctcaa acgtacccaa catattgtgt
      361 gccggggtgt cagacgaggc aatggcagga aatcgtttgt gggaagaatc gtatgggtgg
      421 cgctctcaga acaccgcgcg ggaagctcga tgtcagggtt tgagaaaccc gtttatctcc
      481 gatcaacggg tacccctcgc tgcgaaaacc gctcgtattc ccacaacata cggcaccgtg
      541 atcgaaacct cggtacgaaa ttttcgtgtg acgcggtacc gctgcgcaca tctatctgat
      601 agtagaagtc ttagcgcaca atgcgtaccg aatctcacag aaagatcgtc tgaaaaactc
      661 ggagtgcgcc acccgccaca atgcgtatta cgtcgattcg gccgttggta acccaaggga
      721 gcacgaagat cacgccgatg aggatgaaca tcccgtaccg ctcgaggtcg cgatagcgca
      781 tctcaagatc gcgcgggagg aaatacgcga gtacgcggga gccatccagc ggcggtatcg
      841 gtatcatgtt gaacgccgcg aggatcacgt tgatcatgat gccgaactga aaaaaccgga
      901 tcaccgccat cgtcgcttcc aacggtagta tttccgtcga gtagtatacg tagacggtga
      961 ttcgaagcag aatcgcaaac acgatcgcga agaggatgtt cgatatcggc cccgcaagcc
     1021 cgacccacat catatcgcgg cccggacgct tgaagtatgc cggattcacc ggcacaggct
     1081 tcgcccatcc gaacccgatg atgacgatca tgagcgcacc gatcggatcg atgtgtttga
     1141 tgggattcag cgaaagccgc ccttccatgt ccgcggtcgg atcgccgaag cgtttcgcga
     1201 cccaaccgtg cgatacttca tgcaaggtca ccgccataag gcctatcggt agcaccgtaa
     1261 gcaatatata gggaaggttg tcgagtgaga ggtttccaat cattctattg atatctccga
     1321 cagttttttg gtagatacga tacgtcattt gaaggtacgg aacgtcggaa agtcggtcaa
     1381 cgagaccgcg aacggtcgcc ccgcccgaga gagctccgta tctttgctat cctttgtttc
     1441 gcacaccata ccgcgaaaca ctctctacgc tcgcgaggag gtctgctctg ccgtttctca
     1501 ccatcattct gccgatcttc ctcatattcg gccttggata ccttctcggg cgattccgac
     1561 acgccgagag caaagcgctc gtggatatgg cgctctacct gttcacgccc gcgctgatat
     1621 tctcacgcct tgtggcaaat cctgtatcac ttgtcgatgc gggcaaaatt ggcgtctact
     1681 cgtgggcgct ctgtgcgctg atgtacgtcg ccggatggat cgtcgcgcgc gcgatagggc
     1741 tcgaccggct cgaccgatac gcgctcttgc tcggtgtcgt caccatgaac gcggcgaact
     1801 acggcttacc cgtcgtcgag ttcgccgtcg gaccacacgc cgttgcctat gctgcggtat
     1861 tcatcctcgc cgtgaacatc gtccagagca ccgtcggagt gtatctcgcc gctgcgggaa
     1921 ggcgttcgcc atgggaatcg ttcctcagcg tcttcaagct cccgctcgtc tacgcgtttg
     1981 tcgcggcgtt cgtgatccgt ggactggata tcacgctccc cgaaccgatt ttccgcccga
     2041 tgatgctcct cggtgatgct gcgattcccg tcgcgatggt gattctggga atccaattga
     2101 cgaaggtgca tctcggcggc gcgtggaaga atctcacggc gattaccgcg atccgcctca
     2161 tcctcagccc gttggtcgga ctcgcgctcg catgggcgct cggactccat ggtgacatgc
     2221 gtttcgcgct gatcctcgaa gcggggatgc cgacggcggt gaatgcggga ctacttgcgg
     2281 cggagttcga tacaaaaccg gagttcgtca ccggtgcgat catggtaacc acgctcgcga
     2341 gcgctgtcac gctcagcgtg attatctcgc tgttcggcgg gtagaatctt tagcggtcgc
     2401 gcaccgtctc aggcagtggg attacctcgc gaatccaccg catggtcggc tcaatgaagc
     2461 gcggactgat tcggtcggga tcctcgtgga ggtccggcac cgcatcgcgc agatcgtgct
     2521 tcatgccgag cggaacctcg gcgcgggaca tcgacctgcg ccgtagctgg taccggaacc
     2581 gctcaatgtc cagcatcggc agaagagtat cggcc
//
LOCUS       DSCC01000220            1680 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048827, whole genome shotgun sequence.
ACCESSION   DSCC01000220 DSCC01000000
VERSION     DSCC01000220.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048827"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..>1680
                     /locus_tag="ENN56_02750"
     CDS             <1..>1680
                     /locus_tag="ENN56_02750"
                     /inference="COORDINATES: protein motif:HMM:PF07494.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74436.1"
                     /translation="GSQIVVGLETGIAVGDRRDNLLLPRAWQFIGTDRFAKVRAIVPF
                     ESSVYVASVDGVIRETPLGWETTYALSGANDLTVFDGAVWAATDAGLLQSTDGATWSV
                     AAGLADSMYSVASVPGKMLVVAGASGTWRIESRTDTSPTKVPGVDFPPFTSFRQMSID
                     TTGALWIAPGPAGGEELQNVGFLRFKDNRWTQYVPGTPGIPVPPDRSGYRAIAVDAAN
                     RLWVGSHGSGIVVGDLSTEPPTFQFANRANSGITGLSDTPDYLAVSALHVGTDGYVYA
                     NLYHSLTWAFAPGFVPGMTSGPNQVTAQYLGTEISLTLEPSALAVDPNGVLWIGLRRS
                     GIILIDLNGTPVDPSDDQFAGSIGVGGSVSLPDPNVTSIAIDRDGAVWVGTQGGLTVF
                     TGAYNRNTNTYLLDSRSFSTNNNLPSNVVNAVIVDSANVKWIGTDAGIAQITAARRVI
                     NLSSTRLVDPRGKVVSLVYDHGAGAIWIGTERGLNRYQAYDVLGEGTAVQPSENPYRI
                     GLDRRGNDYVLSGEPLTLLVTPGATLRIYTLTGDLVFEATDTGVGQITWNGT"
BASE COUNT          327 a          462 c          545 g          346 t
ORIGIN      
        1 cggatcacag atcgtcgtgg ggctcgaaac cggcatcgcg gtcggcgacc ggcgcgacaa
       61 cctcttgctt cctcgcgcat ggcagttcat tggaacggac cgattcgcga aggtacgcgc
      121 gattgtgccg ttcgagagca gtgtgtacgt ggcgtccgtc gatggagtca tacgggagac
      181 accgctcgga tgggagacga cctacgcgtt gagcggtgcg aacgacctca cggtgtttga
      241 cggcgccgtg tgggcggcaa ctgacgcagg tttgttgcag agcactgatg gcgcgacgtg
      301 gagcgtcgcc gccggactgg ccgattcgat gtattccgta gcgagcgtac cggggaagat
      361 gctcgtcgtc gcaggcgcgt ccggtacatg gcggatcgaa agtcgcacgg atacgtcgcc
      421 gacgaaggtg ccgggcgtcg acttcccacc gtttacgtcg ttccgtcaga tgagcatcga
      481 tacgacgggc gcgctttgga ttgcgccggg tccggcgggt ggtgaagagc tgcagaacgt
      541 aggcttcctc cgtttcaagg acaatcgctg gacacagtac gtgccgggta cgccgggtat
      601 acctgtaccg cccgaccgat cggggtatcg cgcgattgcg gtggatgctg cgaatcggct
      661 ctgggtcgga agtcacggtt cgggcatcgt tgtcggtgat ctgtccaccg aaccaccgac
      721 gttccagttc gcgaaccgag cgaattccgg cattacgggc cttagcgata ctcccgatta
      781 cctcgctgtg agcgcacttc atgtcggtac cgacggatac gtctatgcga acctttatca
      841 cagtctcacg tgggcatttg ctcccggatt tgtccccggt atgacaagtg gtccgaatca
      901 ggtaaccgcg caataccttg gcacggagat cagtctgacg cttgaaccga gcgcgctcgc
      961 ggttgaccca aacggagtac tgtggatcgg tttgcggcga agcggcatta tcctaatcga
     1021 cttgaatgga acgccggttg atccgtcgga tgaccagttt gcgggaagta tcggtgtggg
     1081 aggatccgtc tcgttaccgg atccgaacgt gacaagcatt gccatcgatc gagacggcgc
     1141 cgtctgggtt ggaacacaag gtggattgac agtgttcacc ggcgcatata accgaaatac
     1201 gaacacgtat ttgctcgatt cgcggtcgtt ctcaacgaac aacaacctcc cgtcgaacgt
     1261 tgtgaacgct gtcatcgtcg actcggcgaa tgtgaaatgg atcggcaccg atgcgggtat
     1321 agcgcagatt accgcggcgc ggcgtgttat caacctttcg tcgacgcgac ttgtcgatcc
     1381 gcggggaaag gtcgtttcgc tggtctacga tcacggtgcg ggtgcaatct ggataggtac
     1441 ggagcgcggg ctgaatcgct atcaggcgta cgacgtgctc ggtgagggga cggcagtcca
     1501 gccgagcgag aatccctatc gcatcggcct cgatcgtcgc ggaaacgact acgtgttgag
     1561 cggtgagccg ctcacgcttc tcgtgacacc gggcgctacg ctgcgaatct acacgcttac
     1621 gggcgacctc gtcttcgaag caacggatac cggcgtcggg cagatcacgt ggaatgggac
//
LOCUS       DSCC01000221            1947 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039923, whole genome shotgun sequence.
ACCESSION   DSCC01000221 DSCC01000000
VERSION     DSCC01000221.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039923"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            177..>1947
                     /locus_tag="ENN56_02755"
     CDS             177..>1947
                     /locus_tag="ENN56_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74437.1"
                     /translation="MKMLRAAVLTLFLATAAHAATVSGIIESTTWTVDQSPYYVVDTV
                     LVAEGAVLNIEAGVDVLFDADVPFLIEGALIASGTETDSVRFLPGESATWQGLSIETG
                     YCHLYYTRISGAEKQLGTPVFDGRGGAIRAVGAVHVDLHNVVLTHNRAFDGGAVYAGD
                     SAHVQMQNCLVIGNAARHAGAGVRVQNDALLEAMNSEFSRNIVDDPSLEINEGGGIMV
                     WNANAVLSQCVIRGNNAGRGGGGGIRAVGGYLELFGCEVSANSIRPTPNFIPYPLDSE
                     SNLSRPMADSHPISIRLDDSVVLPVLPRPAGGLRFGGGIWLSDDASMTMEQCTVAMNT
                     AEETGGIGVHASRASISRSIVYMNEGRNLIGYVDGTIHVEFSNVPAPSSYYPWPGEGN
                     LNVDPLFVDSEAGDFTLRPESPCWFMGAYPLDEGSTPPEPPVLTEVDSDSVSGVLGTM
                     TWTSENSPYRVVGPVRVAPSETLTIEAGVDVLFDADVPFIVNGALTAIGTETDSIRFL
                     PGEAEEWGGLRIIGGESNEIAYTRISGGNADGYLIDGYGGGIFLYNPNTRLVLTHSVI
                     SDNDAFCSGGMIVGGHSWAHISYT"
BASE COUNT          390 a          537 c          590 g          430 t
ORIGIN      
        1 tcggttgttg cgcctttcga gattcaacgt tatcctaaaa tgcgaaagtg gctccgcgcg
       61 accagctccg cgcgaccagc tccgcgcgac cagctccgcg cgaccagctc cgcgcgacca
      121 gtgttgtggt tttctaagcg ttactctcac tgagtaacgt cgcggaggtt acagccatga
      181 agatgcttcg tgcagccgtt ctcacgctgt ttctcgccac agccgcgcac gcggcaaccg
      241 tttccggcat catcgagtcc accacgtgga ctgttgatca atccccctac tacgtcgtgg
      301 acactgtact cgtcgctgaa ggcgccgtct tgaacatcga agccggtgtc gatgtgctct
      361 ttgatgccga tgtgcccttc ctcatagaag gtgctctcat cgcgtctggt accgaaaccg
      421 acagcgtgcg gttcctaccc ggcgaaagcg caacgtggca ggggctcagt atcgaaaccg
      481 ggtactgtca tctgtactac acgcggatca gcggcgcgga aaaacagctt ggaacacccg
      541 tcttcgatgg acgcggtggc gcaatccggg cggtcggagc agtgcatgtc gaccttcaca
      601 acgtggtgct cacgcacaac cgagcgttcg acggcggcgc cgtttacgcg ggcgacagcg
      661 ctcacgttca gatgcagaat tgtttggtca ttgggaacgc cgcacggcat gcgggtgcgg
      721 gcgttcgcgt acaaaacgac gcgctgctgg aagcgatgaa ttcagagttt tcgcggaata
      781 tcgttgacga tccgtcgctg gagatcaacg aaggcggcgg catcatggtg tggaacgcaa
      841 acgccgtatt gagccagtgc gttattcgtg ggaacaatgc cggtcgaggc ggaggcggcg
      901 ggattcgcgc cgtcggagga tatctcgaac tgttcggctg tgaagtgagt gcgaactcga
      961 tacgaccgac tcctaacttt attccgtacc cgctcgactc cgaatcgaat ctttcccgac
     1021 ctatggccga tagtcacccg atatcgatcc gactcgacga ttccgttgta ttgccagtgt
     1081 tgccgagacc ggcggggggc ctgcgattcg gtggcggtat ctggttatcg gacgatgcgt
     1141 cgatgacgat ggagcaatgt accgtcgcga tgaatactgc cgaagaaacg ggtggtatcg
     1201 gcgttcatgc gtcgagggca tcgatcagcc gaagcatcgt ctacatgaat gaaggtagga
     1261 acctcattgg gtacgtcgat gggactattc acgttgagtt ctcgaacgtt cccgcgccat
     1321 cctcttacta tccgtggccg ggtgaaggca acctgaacgt cgatccgttg ttcgttgatt
     1381 cggaagccgg agacttcact cttcgccccg agtcaccgtg ctggtttatg ggtgcatatc
     1441 cactcgatga agggtcgaca ccacccgaac ctccggtatt gaccgaggtc gacagcgatt
     1501 cggtctcagg cgtactgggg acgatgacgt ggacctctga gaactctccg tatcgcgttg
     1561 tcggacctgt tcgcgttgct ccgagtgaga cacttacgat cgaagccggc gtggatgtgc
     1621 tcttcgatgc cgatgtgccc ttcatcgtta atggcgcgtt gacggcgatc gggaccgaga
     1681 ccgacagtat acgattcctc cccggtgaag ccgaggaatg gggcggattg cgaatcatcg
     1741 gcggggaatc gaatgagatt gcgtacactc ggatcagtgg cggtaacgcc gacggttacc
     1801 tcattgacgg gtacggcggc ggcatcttcc tctacaatcc gaatacgagg ctcgtgctga
     1861 cgcattcggt gatttcggac aacgatgcgt tctgcagtgg aggaatgatc gtaggtgggc
     1921 actcatgggc gcacatttcg tatacga
//
LOCUS       DSCC01000222            2203 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033764, whole genome shotgun sequence.
ACCESSION   DSCC01000222 DSCC01000000
VERSION     DSCC01000222.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033764"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..493
                     /locus_tag="ENN56_02760"
     CDS             <1..493
                     /locus_tag="ENN56_02760"
                     /inference="COORDINATES: protein motif:HMM:PF13487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDS74438.1"
                     /translation="LNIAALLHDVGKIGVYDSSLRKTTRLSNEEILQLKEHPTIGAGI
                     IKRIDFFHDIAPIVRSHHERFDGHGYPDGLANDAIPIESRIICVADAYDAITSKRTYN
                     KPLSPLKAVEEIERNSGTQFDPEVVSALRNALEPNDLPTVQLNEDDFEEFEIPNECDP
                     DYL"
     gene            552..1349
                     /locus_tag="ENN56_02765"
     CDS             552..1349
                     /locus_tag="ENN56_02765"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74439.1"
                     /translation="MSRALFGLSTAHIAHDTIATAIDRCVELGTDAVEFFTAEYSVDE
                     CREIRDRARDASLLVDYHAPWHGDNDLGLSPRDTAFHSLEQSIARAQLMSARHLVCHL
                     GRYDLDDPNGRERAIDQVIEITHALEDAVEDADLVLVYEDNTLVHDPNPLGDTPRDFE
                     RLFAAIDNPRIGMILDTGHAHVTGFTRTYLDQFGSRIRYVHLDDNGGIGDDHLPPSAG
                     TIDWEQEFEWFAYYGIEPNFAIEFNESYVAAELPIFRDLAARYSWER"
     gene            1760..>2203
                     /locus_tag="ENN56_02770"
     CDS             1760..>2203
                     /locus_tag="ENN56_02770"
                     /inference="COORDINATES: protein motif:HMM:PF02310.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74440.1"
                     /translation="MASNSVVLINPDREKPLIAPMPLDYLAAALVDAGFEPELLDLAF
                     ESDWYAATNQAVAKRELLAIVILVRHLDDGLYNSQTYFLPKSKTLVEYLRSKTKAPII
                     LIGGAYSLVPEQILRFCRADFGVAGDPEEAVPKLLEALRDGGDLET"
BASE COUNT          505 a          622 c          588 g          488 t
ORIGIN      
        1 gctcaacatc gctgcgctgc tccacgatgt tggcaagatc ggtgtatacg actcctctct
       61 gcgaaaaacg acacgactca gcaacgaaga gatactccaa ctcaaagagc atcctacaat
      121 tggcgcggga atcattaagc gaatcgattt cttccacgac attgcgccta tcgtgcggtc
      181 gcaccacgag cgcttcgatg ggcatgggta tccggacggg ctcgccaatg acgcgattcc
      241 catcgaatcg cgaattatct gcgtggcgga cgcctacgac gccattacca gcaagcgcac
      301 gtacaacaaa ccgctcagtc cactgaaagc cgtcgaagag atcgagcgaa attccggcac
      361 gcagttcgat cctgaggttg tgtccgcact ccgaaatgca ctcgagccga acgatcttcc
      421 cacggtccag ttgaatgagg acgacttcga ggagtttgag attccgaacg aatgcgaccc
      481 ggattacctt taaaacgact acggcttttc tttcaacgtg acattctcca caacgaagag
      541 gaaggtattt catgtcgcgt gccctattcg ggttgagtac tgcgcacatt gcacacgaca
      601 cgattgcgac agcgattgat cgttgtgtcg agctcggcac cgatgcagta gagtttttca
      661 cggccgaata cagcgtcgac gagtgtcgtg agatacgcga ccgtgcgcgc gatgcatcct
      721 tattggtaga ctaccatgcc ccatggcacg gcgataacga tctcggattg tcgccgcgcg
      781 ataccgcgtt tcattcgctt gagcagtcga ttgcgcgcgc gcagctgatg tcggcaagac
      841 acctcgtctg tcacctcggt cggtatgatc tcgatgaccc caacgggaga gaacgcgcga
      901 ttgatcaagt gatcgagata acgcatgcgc ttgaggacgc cgtcgaagac gccgatctcg
      961 tgctcgtcta cgaagacaat acgcttgtgc acgatccgaa tccgctcggc gatactccgc
     1021 gcgatttcga acggctattc gcggcgatcg acaacccgcg catcgggatg attctcgata
     1081 ccggacatgc gcacgtcacg ggattcactc ggacgtacct cgaccagttc gggtcgagaa
     1141 ttcgatacgt gcatctcgat gacaacggtg ggatcggcga cgaccatctc ccaccgagtg
     1201 ctggcacaat cgactgggaa caggaatttg agtggttcgc atactacggc atcgaaccga
     1261 acttcgcgat tgagttcaac gagagctatg tagcggcgga gttgccgata ttccgcgatt
     1321 tggcggctcg ctattcgtgg gagcgataac gcggtgcggc gtatccgata gacctgcgtt
     1381 tttcggtttg ttcagagcgt tcccccacgg caacgaatac cgaacgtctc cacgttcggc
     1441 tcaaatcgat tacgaagatg tcgggacgcg ttgaataccc ccttgtaccg gaattcgctg
     1501 cttcattttt gacaatcacg acccgaatgt gttatcttct ctattcaacg agtaaacacg
     1561 tgaatactcc acactatgac cacgccaatt gttggaaacc tttccgacat gccgcatatg
     1621 ctacgcaact ccgacggccg taaccgtgga aaccactcgg gtacgagtcg gcagacagga
     1681 gtagtttcgc cgtatacgaa ctcctcgatg tattcggtcc gttgtgtagt gcggtaattc
     1741 cgttatcagg aggacaccag tggcgtcgaa ttcggtcgtt ctgatcaacc ccgatcgcga
     1801 aaaaccactc attgcaccca tgccgctcga ctacttagcg gcggccctcg tcgacgccgg
     1861 ctttgagccg gaactgctcg atctcgcgtt tgagagtgat tggtacgctg cgacgaatca
     1921 ggctgtagca aaacgcgagt tactcgcaat cgttatcctc gtacggcatt tggacgacgg
     1981 actgtacaac agccagacgt atttcctccc gaagtcgaag acactcgtcg aataccttcg
     2041 cagcaagacg aaggcaccga ttatccttat cggtggtgca tattcgctgg ttcccgagca
     2101 gattctccgc ttctgtcgcg cggatttcgg cgtggcggga gaccccgaag aagcggtgcc
     2161 gaagcttctc gaagctcttc gagacggtgg cgatcttgag acc
//
LOCUS       DSCC01000223            1610 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1051737, whole genome shotgun sequence.
ACCESSION   DSCC01000223 DSCC01000000
VERSION     DSCC01000223.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1051737"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..539)
                     /locus_tag="ENN56_02775"
     CDS             complement(<1..539)
                     /locus_tag="ENN56_02775"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS74441.1"
                     /translation="MRVTFRGTRGSYPVAGPDTVRYGGNTSCIEVRLDDGTLIILDAG
                     SGLVSLGASLTAPDASDGFHRGDGDATILLTHTHWDHIMGMPFFAPATVAGNRFRVLG
                     RRKECEKISLEEVFRGQQIKDYSDRSFDDFPATFEFHEIVEGDTFDVGSASVATARLN
                     HPCYALGYRITADHASVVY"
     gene            complement(599..>1610)
                     /gene="dgoD"
                     /locus_tag="ENN56_02780"
     CDS             complement(599..>1610)
                     /gene="dgoD"
                     /locus_tag="ENN56_02780"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:PRK14017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDS74442.1"
                     /translation="GDRAQTIAQAILELKRYLVGKDPLRIEYHWQAMYRGAFWRGGPI
                     LNAAISGVDIALWDILGKRLDAPVWQLMGGKTRERVRFYTGIGGDTPEAVANAAKTAI
                     ARGFTAVKWCPVPATEPLAGMRGPKNAAAQIEAVRNAVGIDVDVLLDFHGRLSPAMAI
                     VYAKAVEPWHPMFIEEPVLPEHSNEIPRIAAATSVPIATGERLFTKYGFRPILEAGGV
                     GMIQPDLSICGGLTEGKKIAAMADAYGVGVGPHDPYGPVLTAASLQLDACTPNFVIQE
                     HVSLGEEIFHKPLTLVDGYVIPPEGPGLGIDLDEKAVRERVYEPQDVPLLFHEDGAIA
                     DW"
BASE COUNT          356 a          488 c          413 g          353 t
ORIGIN      
        1 atatagacaa ccgatgcgtg atcggcggtg atgcggtatc caagggcgta acacgggtgg
       61 ttcaggcgtg ccgtcgcgac tgaggccgaa ccaacatcga atgtatcgcc ctcaacgatc
      121 tcgtggaatt caaacgtcgc tggaaaatcg tcaaatgacc gatcggagta atccttgatt
      181 tgctggccac ggaacacctc ttcgagcgaa atcttctcac attcctttcg ccggccaaga
      241 acacggaacc ggtttcccgc gaccgtcgcc ggtgcgaaaa acggcatccc cataatgtga
      301 tcccaatggg tatgtgtcag aagaatcgtt gcgtctccgt cgccgcgatg gaatccgtcg
      361 gatgcgtctg gagccgtgag cgaggcgccg agtgatacca ggccgcttcc cgcatcgaga
      421 atgatgagtg ttccgtcatc gagtcggacc tcgatacacg atgtgttccc tccatatcgg
      481 acggtatccg gtcccgcgac cggatacgat ccacgcgtac ctcggaacgt gactcgcatg
      541 tacgcatctc ccccattact ctgttcgtgt atttccgtaa tgcaaaaacg tattcgtgtt
      601 accagtccgc gatagcaccg tcttcgtgaa agagtagagg tacatcctgt ggctcgtaaa
      661 ctctttcgcg aacagccttt tcgtctaaat cgattccgag acccggacct tcaggtggaa
      721 tcacgtatcc gtcgacaagc gttagcggct tgtggaaaat ctcttcaccc aaactcacgt
      781 gttcttgaat gacgaaattc ggcgtacatg cgtcgagctg caaggacgca gccgtcaata
      841 ccggtccgta gggatcgtgc ggcccgactc cgacaccata cgcatcggcc atggcggcga
      901 tctttttccc ctctgtaaga cccccgcaga tactcaaatc cggctgaatc attccaacgc
      961 caccagcttc aagtatcgga cggaatccgt attttgtaaa taggcgttct ccggtcgcga
     1021 tcggaacact tgttgccgcg gcaattcgcg gaatctcatt tgaatgctcc ggtagcacag
     1081 gctcttcgat gaacattgga tgccacggct cgaccgcttt cgcgtacacg atcgccatcg
     1141 cggggctcaa tcgcccgtgg aagtccaaca acacatcgac atcaatacct acggcgttgc
     1201 gaactgcttc aatctgggct gccgcatttt ttggaccgcg cattccagca agcggttcgg
     1261 tcgcaggaac cggacaccac tttaccgcag tgaaaccacg agcaatagcc gtttttgccg
     1321 cattcgcaac cgcttccggc gtatcaccac cgatgccggt gtagaaccgc acccgttcgc
     1381 gcgtcttgcc acccataagc tgccagacgg gcgcgtcgag acgctttcca aggatgtccc
     1441 agagcgcgat atccacgccg ctaatcgccg cgttgaggat tggaccacca cgccagaacg
     1501 cgccgcgata catcgcttgc cagtgatact cgattcggag cggatccttt cctacgagat
     1561 accgcttcaa ctcaaggatt gcctgcgcga ttgtctgagc gcgatcgccc
//
LOCUS       DSCC01000224            2053 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037155, whole genome shotgun sequence.
ACCESSION   DSCC01000224 DSCC01000000
VERSION     DSCC01000224.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037155"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
BASE COUNT          477 a          607 c          615 g          354 t
ORIGIN      
        1 gtcacggtgg aagcggagtt tgacgagccg gtaacactgg aagtcgccgg atttacggga
       61 cccgcaacgg ttccgatcat tccggagatt cacgcgacgt cggccacacc ggaacgagcg
      121 catcatatga ccgtccgtgg gctgacgcag aacacgccgt atacattcgt gatcaagtac
      181 accgacaatg ccggcaactc ggcgacgtgg ccggaaggca cggtgctcgc ccgtacggca
      241 cgtggtcttg agatcacgaa gctgacgcag ggacgcggca attcattcac gacgagtccg
      301 agtgcggacc tcacaccgcc gcagatcgtc aagttgccaa aaataacgtc ccggtccacg
      361 aactcgatca ccatcgtgtg ggaaaccgat gaaccagccg acagccgcgc gcggttcgca
      421 cgtatcgata atcttccacc ggctgcaaag gccgcccgtg caacagacgg caccgactac
      481 accgacgagg tgatcatcac cgagacgacc acggaacacc acatcgtcat cacgcagctc
      541 gatacatcgg gcacgtatga gatcgagatc gcatcggtgg acgtgagcgg gaacggtgaa
      601 accaccgcgc cgccggtcac gacgacaacc acggtaacgg ccgatgtcac gccgccggtg
      661 atcgtgagtg gtccgacggt gctttcgaag acgcatgatc gcgcggtggt tcgttgggtg
      721 accgatgagt cggctgattc gcaggtggac gtgctcgaac gcggtcagcc gctcgataag
      781 cggtctacgt tcaacacagg tgagttcggc actgagcatg tggtcacgtt gacgaaccta
      841 aaggcgaata cgctctacga ttacctcgcg agcagctacg atgtcgagca aaacggtccg
      901 gtaacagcgc tcggtgagtt caccacgctg gagacgccgg ataccgatcc gccagtgatt
      961 gtgtccgcgc cgacggtggt caagttcgac gacatctccg cgcaactggc atgggagacg
     1021 aatgaagcga ccgacaccta catcgaatac ggtacgacga cgtcgtacgg catggtcgcg
     1081 cagagcgcga cgaagcgcac ggatcactcg atcacgttga cgaatttgag cgagagaacg
     1141 cagtactatt acgcggttca ggcggaagac gccgctggga acgtctacac ccagacgacc
     1201 cccggcacgt ttaccacagc agatggtccg gacgtgacac cgcctgcggc gcctgcgaac
     1261 cttacagcag ttccaggtga cggtgaggtg tacgtggctt ggcagatgaa caacgagccg
     1321 gatttcgccg gatacgagat cgaacgtgct gtgcaggggc agagcttcgc cacggtcgcg
     1381 acgaacatcg taacctcgca gtatctcgat cagagcaccg tgaacgacgt ggcgtatctc
     1441 taccgcatcc gtgcggtgga cgactcgcgc atgcacaaca aatcggcata cgcgacaacc
     1501 gacgccgtta ctccggaagc cggtttggga ccggaaccgc cggaattcgg accgtattac
     1561 ttcctgtact acaacatcca gacggcggaa ggcgcgctgt tccgaccggt gttcaaggtc
     1621 acgaatgcgt caccgccgcc ggaacaacgg attcaatcgt atcagttcgt tgttgcgaca
     1681 gacgcggcgt tcaagaacgt gatcgtaacg agtcagcagg taccgcccgg cgacgagatt
     1741 atcggaacgc aggaatcgtg gtactgggcg gcgctcaatg tcgaaatgcc ggacgagaat
     1801 cgggatcaca cgcggatgat accggatccc atgtcaacgt catggattcc gacacgaccg
     1861 ttgacgcccg gacagtccta ctactggaag gtgcgcacgt acgacggcat tttcttcagc
     1921 ccgtggagcg atacgtacga gttcggctcg acatcgccgg tagtgctgct cgatccacgt
     1981 cgcgcggagc atttcaacat caatctcaat ccggaatcgc cgccggaacg tccggtctcg
     2041 gtggaacttg cta
//
LOCUS       DSCC01000225            3011 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022254, whole genome shotgun sequence.
ACCESSION   DSCC01000225 DSCC01000000
VERSION     DSCC01000225.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022254"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..361)
                     /locus_tag="ENN56_02785"
     CDS             complement(<1..361)
                     /locus_tag="ENN56_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74443.1"
                     /translation="MTNSYTLWNPEVHVPTRDELVRPDGAWDVLVHHANTDRYHFLHD
                     TSVAMHNGVLHTAWYNCPDREIQDESLIRGRRSSDGGRTWSDVETIAKDETGEGLHYV
                     PPQLFTSDGTLYSLIGIM"
     gene            585..1838
                     /locus_tag="ENN56_02790"
     CDS             585..1838
                     /locus_tag="ENN56_02790"
                     /EC_number="3.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011822287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HDS74444.1"
                     /translation="MASQVKNPALADQGHRNIEWAERQMGALLKVKERFENEKPLAGV
                     KVGLALHVTKETAVLVKTLVAGGAEVVITGCNPLSTQDDVAAALAEMDGVEVFAHKGE
                     TREEYYDFLNRVVKAKPDVTIDDGCDLVTVIHQEYPEMIPDLICGCEETTTGIIRLKA
                     MEADGALKYPVFSVNDSNTKHLMDNFYGTGQSSLDGILRASNILFAGKIVVVVGYGSC
                     GKGVASRARGLGAKVIVTEVDPFRALQATMDGFEVMPMVDAVKVGEVYITVTGNKHVI
                     SAEHIRAMKDGAILANSGHFDNEIDVNWLEKNNSGKRNIRPFFDEYTIGDKVVFLCGE
                     GRLVNLAAAEGHPSTVMAMSFCNQALAVEYGVKNRDNLKPGVLNLPANIDSDIAALQL
                     EAMEIRIDTLTDEQIKYLSSWNEGT"
     gene            complement(1911..2303)
                     /locus_tag="ENN56_02795"
     CDS             complement(1911..2303)
                     /locus_tag="ENN56_02795"
                     /inference="COORDINATES: protein motif:HMM:PF01887.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74445.1"
                     /translation="MRAITAMVLLLVLAVGCASNDESVQDESGEVSLTGIVTEIGGHG
                     NAYTNLDNAALGTVGIGIGDRILVSFPDTPVILTVGTNYDDVPDGENVAVLHREGSVV
                     FAIFNGDFHGAYGVTPGTEFTITPAPVE"
     gene            2535..>3011
                     /locus_tag="ENN56_02800"
     CDS             2535..>3011
                     /locus_tag="ENN56_02800"
                     /inference="COORDINATES: protein motif:HMM:PF11175.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDS74446.1"
                     /translation="MSLHGSSITNLARAKSFRAKRESSWDRTGGNSDWVPIKGQSKHV
                     IAEIDGPGVISHIWFTIAGSGRDKDYLRKMVLRIWWDGEANPSVETPVGDFFGVGHGA
                     VTSFQNALFNMSAHETKVGGNAAMNCWIAMPFRAHVKIEIENESEQDIGMYYYIDYQ"
BASE COUNT          695 a          838 c          861 g          617 t
ORIGIN      
        1 ccatgatgcc gatcaacgag tacaacgtcc catccgacgt gaacaattgc ggaggcacgt
       61 aatgcaagcc ctcccccgtt tcgtccttgg cgatcgtttc aacatccgac cacgtccggc
      121 cgccgtccga tgatctccgt ccgcgaatca atgattcgtc ctgaatctct cgatccgggc
      181 agttgtacca cgcggtgtgc aaaacgccgt tgtgcatggc gacgctcgta tcgtgcagga
      241 agtggtatcg gtcggtgttc gcgtgatgta cgagcacatc ccacgcgccg tccggtcgga
      301 cgagttcatc gcgcgtcgga acgtgtactt cgggattcca gagtgtatac gaattcgtca
      361 tggcagtcct cacgagtgga acgcgaaacg cacacatgcg tttggtacgc agattgtact
      421 cggattctat gaagtcccgc aacccgggcg gcgtacgacg ccgcattccc ggaaggattt
      481 gtcggaccgt ttgtttctat taggacaaca cggacatagg caacagacac cgcggcgctg
      541 aagcagcgcg cggttcacta cagatcacag gaggacaccg ttttatggca tctcaggtaa
      601 agaatcctgc gctcgccgac caaggtcacc gcaatatcga gtgggcggaa cgacagatgg
      661 gcgcgctgct caaggtcaaa gaacgtttcg agaatgaaaa accgctcgcc ggagtaaagg
      721 tcgggctcgc actgcacgtc acgaaagaaa cggccgtgct tgtgaagacg ctcgtcgccg
      781 gcggagcgga agtcgttatc accggatgca acccgctctc gacacaggac gacgtcgccg
      841 cggcacttgc cgagatggac ggcgtcgagg tcttcgcgca caagggcgag acgcgcgaag
      901 agtattacga tttcctcaac cgcgtggtga aagcgaagcc ggatgtcacc atcgacgacg
      961 gctgcgacct cgtcacggta atccatcagg agtatccgga aatgattccg gacctcatct
     1021 gcgggtgtga agagaccacc accggaatca tccggctcaa ggcgatggaa gccgacggcg
     1081 cactgaagta tccggtgttc tcggtgaatg acagcaacac caagcacctc atggacaact
     1141 tctacggtac cggtcaatcg tcacttgatg gtatcttgcg cgcatcgaac atcttgttcg
     1201 cgggcaaaat tgtcgtggtt gtcggatacg gctcgtgtgg caagggtgtt gcgagccgcg
     1261 ctcgcggtct cggcgcgaag gtgatcgtga ccgaggtcga cccattccgc gcacttcaag
     1321 cgacgatgga cggcttcgag gtcatgccca tggtggacgc ggtgaaagtc ggtgaagtgt
     1381 acatcaccgt cacgggcaac aagcatgtca tctctgcgga gcatatccgc gcgatgaaag
     1441 acggcgcgat tcttgcaaac tcgggtcact tcgacaacga aatcgacgtc aactggctcg
     1501 aaaagaacaa ttccggcaag cggaacatcc gcccgttctt cgacgaatac accatcggcg
     1561 acaaggtcgt cttcctctgt ggtgaaggcc gcctcgtgaa cctcgccgct gccgaagggc
     1621 atccctcaac cgtaatggcg atgtcgttct gcaaccaggc gctcgcggtg gaatacggcg
     1681 tgaaaaaccg cgacaacctc aagccgggcg ttctgaatct tccggcaaac atcgattcgg
     1741 acattgcggc gcttcagctc gaggcgatgg agattcgcat cgatacgctc accgacgagc
     1801 agatcaagta cctcagctcg tggaacgaag gaacgtaatc gatgcgctcg cgcgtacgga
     1861 aaacggagcg acggcaacat accgtcgctc cgtttttttc ggtatacggg ttattcgacc
     1921 ggagcaggtg tgatggtaaa ctccgtgccg ggagttacac cgtatgctcc gtggaagtcg
     1981 ccattgaaga ttgcgaacac gacggatccc tcgcggtgca gaactgcgac gttttcgcca
     2041 tcggggacat cgtcgtagtt cgtgccgacg gtgaggataa ccggtgtatc tggaaacgac
     2101 acgagtatcc ggtcgccaat acctatacct acggtgccga gtgctgcgtt atccagattc
     2161 gtgtatgcgt tcccgtgacc accgatttcc gtcacgattc ccgtgagcga aacttcgcca
     2221 ctctcgtcct gcacggactc gtcgtttgac gcgcaaccca cagcaagtac gagtaggagt
     2281 accattgcag taatggctcg catgttcgtt cctttcctct gtcgttatgc atttcgtcga
     2341 cctgtttcca ttatacgatg ataccatctc agaatgaacg cagtcggaac ggcagacgag
     2401 acgaaatcgg acgacgctac gtgtgttttc cgccactgtt taacggaata cccttccccc
     2461 aatctgcata ctgagtcact ctttaggctg ataataaacg acctacacaa ccagacagcg
     2521 tgaggaactt caccatgagc cttcacggaa gcagtattac taatctcgcg cgcgcgaagt
     2581 cgttccgcgc caagcgtgaa tcgagttggg accgcaccgg tggcaactcc gattgggtac
     2641 cgatcaaagg gcaatcgaag cacgtgattg ccgagatcga tggtccgggc gtgatcagtc
     2701 acatctggtt caccatcgcg ggcagcggac gagataaaga ctatctccgc aagatggttc
     2761 tgcgcatctg gtgggacggc gaagcgaacc cgtcagtcga aacgccggtt ggtgatttct
     2821 tcggcgtagg gcatggggcg gttacgtcat tccagaacgc actgttcaac atgtcggcgc
     2881 acgagacgaa ggtcggagga aatgccgcga tgaactgctg gatcgcgatg ccgttccgcg
     2941 cacatgtgaa gatcgagatc gaaaatgagt cggagcaaga tattgggatg tactattaca
     3001 tcgattacca g
//
LOCUS       DSCC01000226            2575 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1027374, whole genome shotgun sequence.
ACCESSION   DSCC01000226 DSCC01000000
VERSION     DSCC01000226.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1027374"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            9..563
                     /locus_tag="ENN56_02805"
     CDS             9..563
                     /locus_tag="ENN56_02805"
                     /inference="COORDINATES: protein motif:HMM:PF00092.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDS74447.1"
                     /translation="MFLREINIGTVPEEGTDLARAIETGLGAFIEDVDARNRVMIVFT
                     DGESHDSPATLRAASKAAERAVRIYTVGIGSSDGDLIPIHRNGRADYLRDDQGNVVKT
                     RLDDVTLRRVAAETDGAFYRASGTGGELQEILDDINELERTRIAEEQLVTRVDRYQWP
                     LGVAISCLLIVPFVPERRRTGGRT"
     gene            482..1225
                     /locus_tag="ENN56_02810"
     CDS             482..1225
                     /locus_tag="ENN56_02810"
                     /inference="COORDINATES: protein motif:HMM:PF13424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS74448.1"
                     /translation="MAAWCRYLMSIDRSICAGTSSNRRSNVIRSLRFPIVATFILGSL
                     VACDAVYDPVGLRVREGVEHYAAGRHEEALAAFREAQIDAPNDRRIAMNIGNTLYALG
                     KYDEAEEAFTEALGSSRSETRSHASYNLGNTRFRRSQLAQAAENYERTLEIDPDDMDA
                     KYNLELVQLLLNQAASESDQQQQEQRVVSDWAKQRAREAEALARQRRYADAFRLMRRT
                     ADTEQAAGEEYADFIERLSDLALIFESDS"
     gene            1225..1791
                     /locus_tag="ENN56_02815"
     CDS             1225..1791
                     /locus_tag="ENN56_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74449.1"
                     /translation="MIAMRASITAIAVFALCNGAIASTDRSDERVANAAELFGSVQFD
                     SEYEALALRYLAEALAEDPANREAQQLAEAIQRRRSEREEQERQRGQRQIDQREQERS
                     SEEREDSRENNQQTDEEQEQAESADDRDDRVEQVEEQSTEDAPEQDPVEDEMTHEEAL
                     RLLDALEAQEKTPQVRPQKQSGSRGPRW"
     gene            1785..>2575
                     /locus_tag="ENN56_02820"
     CDS             1785..>2575
                     /locus_tag="ENN56_02820"
                     /inference="COORDINATES: protein motif:HMM:PF13584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74450.1"
                     /translation="MVTDVYLDAATRYLRAVCIANVVTVFLVSASIAAPTIEATINRN
                     VLFVGEQAALTISVTDERGHDVSVPEVPSVRNLDVRGPFGPSTSREFQFIQGRAAQKA
                     TTSYTFTIIPREAGQYQIPAIDVKVSGDTYQTQPISLTVEESAPSKGAKQARPDIYVQ
                     LTVSADTAYQGEAIIVEYALVESAEISVSSRVITQEPEFTAAWVEKLFDAREDQSTST
                     ERIENGIRRRVLPILRVVLFPTKSGELQIPEMAFSASVATPTGRR"
BASE COUNT          642 a          664 c          774 g          495 t
ORIGIN      
        1 cgatacggat gttccttcga gagatcaata ttggcaccgt gccggaagaa ggaaccgatt
       61 tggcgcgcgc gattgagacc ggtctcgggg cgtttattga agacgttgac gctcgcaacc
      121 gtgtaatgat tgtgttcacc gatggtgaat cacacgattc tccggcgacg ctacgagcgg
      181 cctccaaagc agcggagcgt gcggtgcgca tctacacggt cggcattggt tcgagcgacg
      241 gtgatctgat tcccattcac cggaatggac gcgccgacta tctacgagac gatcagggaa
      301 acgtcgtaaa aacgagactc gatgatgtga cgcttcgtcg cgtcgcggcg gaaaccgacg
      361 gagcgtttta ccgcgcgagt ggaactggcg gggaactgca ggagattttg gacgatataa
      421 acgaattgga gcgtacccga attgcggagg aacaactcgt cacccgcgtc gatcggtacc
      481 aatggccgct tggtgtcgct atctcatgtc tattgatcgt tccatttgtg ccggaacgtc
      541 gtcgaaccgg aggtcgaacg tgattcgatc gctccgtttc ccaatcgtag ccaccttcat
      601 actcggcagt ttggttgcct gtgacgccgt ttatgacccg gtcggtctac gcgttcgaga
      661 aggagtcgaa cactacgcgg ccggtcggca cgaggaagca cttgcggctt ttcgggaggc
      721 gcagatcgat gcgccgaacg acagacgtat cgcgatgaac ataggaaaca cgctctacgc
      781 actcggaaag tatgacgagg cggaggaagc gtttaccgaa gcgcttggat cgagccgaag
      841 cgagacgcgc tctcacgcgt cgtataatct tgggaacacg cgattccgga ggagccagct
      901 tgcacaagcc gccgagaact acgaacggac tctcgagatc gatccggatg acatggacgc
      961 gaaatacaat ctcgagcttg tgcagctcct cctcaaccaa gccgcttcag agtcggatca
     1021 gcagcaacaa gaacagcgcg tagtgagcga ctgggcgaaa caacgtgcgc gcgaagccga
     1081 ggcgcttgca cgacaacgtc ggtacgccga cgcgttccga ctcatgcgtc gaacggctga
     1141 taccgagcaa gccgctggtg aagagtatgc cgacttcatt gaacgactga gcgatcttgc
     1201 attgattttc gagagcgatt cgtgatgata gccatgcgcg catcaatcac cgcaattgcc
     1261 gtattcgcac tgtgtaacgg agcgatcgcg agtacggacc gctcggatga gcgtgttgcg
     1321 aacgcggctg agctcttcgg ctcggttcag tttgactcgg aatacgaagc gttggcgcta
     1381 cgatatctcg cggaagctct ggctgaggat ccagcgaata gagaagcgca gcagttagct
     1441 gaagcaattc aacgacggcg gtccgagcgc gaagaacagg agcggcaacg agggcaacgg
     1501 cagatcgatc aacgagaaca agagcgatcg agtgaggaac gagaggattc gcgagaaaac
     1561 aatcaacaga cggatgagga gcaagagcaa gctgaatcgg cggatgaccg cgatgatcgc
     1621 gttgaacagg tagaagagca atcaacggag gacgcgccgg agcaagaccc cgtcgaagat
     1681 gagatgactc acgaggaagc tcttcgttta ctcgacgcat tggaagctca agagaaaacg
     1741 ccgcaggttc gcccgcagaa acaaagcggt tcgcgaggtc cgcgatggta actgacgtgt
     1801 acctcgacgc tgcaacgaga tatcttcgcg ccgtatgtat cgcgaacgtg gttactgtgt
     1861 ttctggtatc tgcatccatc gctgcgccga cgattgaggc gacgatcaat cggaacgtgc
     1921 ttttcgtcgg tgaacaggca gcacttacga tatcggtgac cgacgaacga gggcacgacg
     1981 tttcggtgcc ggaagttcca tcggtgagaa atctcgacgt gcgcggtccg ttcggacctt
     2041 ccacatcgcg ggagtttcag ttcattcaag gacgggcggc gcagaaggcg acgacgtcat
     2101 atacattcac catcataccg cgagaagcgg gtcaatatca gatccccgca atcgatgtaa
     2161 aagtttccgg cgatacgtac caaacacagc cgatatccct tactgttgag gaatctgcac
     2221 catcaaaagg tgcaaaacaa gcgcggcccg atatttacgt gcaattgacg gtaagcgcgg
     2281 atacggcata tcagggtgaa gcgattatcg tcgaatacgc gctcgtcgaa tcggctgaga
     2341 tcagtgtttc gagcagagtg atcacacaag aacccgaatt taccgctgcg tgggttgaga
     2401 agctttttga cgcgcgggaa gaccaaagca cgtctaccga acgaatcgaa aacggtattc
     2461 gccgacgagt gcttccgata ttgcgggtag tgctcttccc caccaagtct ggagagctac
     2521 agataccgga aatggcattc tcggcgagcg tggcgacgcc aaccggtcga aggag
//
LOCUS       DSCC01000227            2676 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025997, whole genome shotgun sequence.
ACCESSION   DSCC01000227 DSCC01000000
VERSION     DSCC01000227.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025997"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="ENN56_02825"
     CDS             complement(<1..1298)
                     /locus_tag="ENN56_02825"
                     /inference="COORDINATES: protein motif:HMM:PF05729.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HDS74451.1"
                     /translation="MTGSAALTCRMHDPERPGVYTDHHLWWKGGEGVIECVEAPQRIR
                     PGRSVRLPAVTFARNSGTLQLRWESDAVRCEWRQSARCAVDNEQSAIFSLRPDNAPTC
                     EADFCVVNEAVTEPHDTYPRLHVGRVSLAHWFVALTHTVSLTGVAIGIVGLIWLLFNS
                     CDSAILMLLGGSTVAILSNPFLLRNLNRKVEACLPSQTEHLGTISIPRIVIEPAIIHS
                     TAVSSPVIRAYCESVVRQITDLHVLGIAVPVSAEAGYIPIALRTGRITQVPSSVPKEA
                     YTEIDRIINQDKRLKNLLDGTRHSLEEAWQLHRYLTIVGDAGSGKTTALRRLSHRLAA
                     GAVHWTVSVPVYLELHRVARRPDVQSDPVGALRAATIDRIEEILHRDSEGGDEERSLP
                     SRDDIARVVDLLITNGELTRLLDGLDEVPDDNSTNGESSV"
     gene            complement(1461..2663)
                     /locus_tag="ENN56_02830"
     CDS             complement(1461..2663)
                     /locus_tag="ENN56_02830"
                     /inference="COORDINATES: protein motif:HMM:PF07748.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74452.1"
                     /translation="MGHMVTGVNQMENEFLRVKFNSDGTLRMENKETGDVFDGMHYLE
                     DAGENGHSWMHESPNRDEIITSHAAPCHIAKIEDGPMLARFRVDYHMMVPVGLDYVYD
                     EAYTGQKPRAQMRLPRATTRTEEKREMVVRSVFTLRKGAKALDVETKVDNQCENHRVR
                     VCFPTEIKAKVSAAEAAFDVIERDVIRTPANWYWGRPNPQLPMHRFVSFSDGKRGIAF
                     ANNGIREFEAIEDNDRTVAITLFRAFTFRQPPILDRYDIYPEMKLSQCPGEMSFKYSI
                     LPHKGDWVEGEVIREAERVNLPLETAQVGPHKGDLPKSMSFLSIDPVNLMLSTFKKAE
                     RSDSLILRVFNPTTDDIDGTITFHTEIKTAWITNLNEERREQLQPDGKKINLKVGKKK
                     IVTLELEV"
BASE COUNT          519 a          760 c          805 g          592 t
ORIGIN      
        1 tctacggacg attcgccatt cgtcgaatta tcgtcgggta cttcgtcgag tccatcgagg
       61 aggcgtgtga gttccccgtt cgtgatgaga agatcgacga cgcgcgcaat gtcgtcgcgt
      121 gatggcaaac tccgttcttc gtcgccgcct tccgaatcgc gatgcaagat ttcttcgata
      181 cggtcgatcg ttgcagcacg gagtgcgccg acgggatcgc tctgaacgtc cgggcggcgc
      241 gcgacgcggt gcagctcgag gtataccggc acgctgacgg tccaatggac cgctccggcc
      301 gcgagccgat gagagagtct acggagtgcg gtggtcttgc ctgagccggc atctccgaca
      361 atggtcaagt agcgatggag ttgccacgcc tcttcgagac tgtgacgcgt accgtcgagc
      421 aggttcttca accgcttgtc ctgattgatg atgcggtcga tctccgtgta cgcttctttt
      481 ggaacgctac tcggtacctg agttattcgt ccggtgcgca gcgcgatcgg tatgtacccc
      541 gcctcggcac tgaccgggac agcgataccg agcacgtgta gatcagtgat ctggcggacg
      601 acgctctcgc agtacgcgcg gataacggga gacgagactg ctgttgaatg gataattgcg
      661 ggttcgatga caatgcgcgg aatggagatc gtgccgagat gctcggtttg cgacgggaga
      721 cacgcttcga ctttccggtt caggttccgt agcaggaagg gattcgatag gatggcgacg
      781 gtggatcctc cgagcaacat gaggatcgcc gaatcgcaac tattgaacag cagccatatg
      841 agaccgacga ttccgattgc gacgcccgtc agcgaaacag tgtgtgtgag cgcaacgaac
      901 caatgagcga gtgacacgcg cccgacatgg aggcggggat acgtatcatg cggttccgta
      961 accgcttcgt ttaccacaca gaaatccgct tcgcatgtcg gcgcgttgtc cggtctaaga
     1021 gagaagattg cggattgttc gttgtcgact gcgcatcgcg cggattgacg ccattcacat
     1081 ctcacggcgt cggactccca ccggagttgt agcgtaccgg agttccgcgc aaaggtaacc
     1141 gcgggcagcc tgaccgatcg accgggacga atgcgctgcg gcgcttcgac gcattcgatc
     1201 acgccttccc cacctttcca ccagagatga tggtcggtat atacgccggg ccgttccgga
     1261 tcgtgcatac ggcacgtcag tgccgcgcta cccgtcatgg atgactgcct taagatgaaa
     1321 cgaaggttga agtcgatcga ttcctcagat caatggtagg gaatacgacg cgcgattaca
     1381 agaacgcata ccacggggaa gggcgtgcaa ccggttgccg gttacacgcc ctgttgaacg
     1441 tactggttga cgtactctca ttacacttcg agttcaagcg tgacgatctt cttcttccca
     1501 accttcaagt tgatcttctt accgtcgggc tgaagctgct cacgacgctc ttcgttgagg
     1561 ttcgtgatcc acgcggtctt gatctccgtg tggaacgtta tcgtgccgtc gatatcgtcc
     1621 gtcgtcggat tgaagacgcg cagaatgaga ctatcgctcc gctcggcctt cttgaacgtc
     1681 gaaagcatga ggttgaccgg gtcgatcgag aggaagctca tcgacttcgg taggtcaccc
     1741 ttgtggggac cgacttgcgc ggtttcgagc ggcagattca cgcgctcggc ttcgcggatg
     1801 acttcgcctt ccacccagtc gcctttatgc ggaaggatcg agtacttgaa cgacatctcg
     1861 cccgggcact gcgagagctt catctccggg tagatgtcgt accggtcaag aatcggcggc
     1921 tgacggaagg tgaacgcgcg gaacagcgtg atcgccaccg tgcggtcgtt gtcttctatc
     1981 gcctcgaact cgcgaatgcc gttgttggca aatgcgattc cgcgcttccc atcggagaag
     2041 ctcacgaacc ggtgcatcgg aagctgcgga ttcggacgac cccaatacca gttggccggt
     2101 gtgcgaatca cgtcgcgttc gataacgtcg aacgcggctt cggcagcgga gaccttcgcc
     2161 ttgatctctg tcgggaagca gacgcgcacg cgatgattct cgcactggtt gtctaccttc
     2221 gtttcaacgt cgagcgcctt cgcgcccttg cgcagcgtga agacggagcg aacgaccatc
     2281 tcgcgctttt cttccgtgcg cgtcgtcgcg cggggcagac gcatctgggc gcgcggcttc
     2341 tgaccggtgt acgcttcgtc gtagacgtaa tcgagaccga ccggaaccat catgtggtag
     2401 tcgacgcgga agcgcgcgag catcggaccg tcttcgatct tcgcaatgtg acacggcgcc
     2461 gcgtggctcg tgatgatctc gtcgcgattg ggcgattcat gcatccatga atgtccgttt
     2521 tcaccggcat cttcgaggta gtgcatgccg tcgaagacat cacccgtctc cttgttttcc
     2581 atccggagcg taccgtcgga gttgaatttc acgcggagga actcgttctc catctggttc
     2641 acgccggtga ccatgtggcc catcggcttc cagaca
//
LOCUS       DSCC01000228            4167 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1014379, whole genome shotgun sequence.
ACCESSION   DSCC01000228 DSCC01000000
VERSION     DSCC01000228.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1014379"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            44..1591
                     /locus_tag="ENN56_02835"
     CDS             44..1591
                     /locus_tag="ENN56_02835"
                     /inference="COORDINATES: protein motif:HMM:PF00593.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS74453.1"
                     /translation="MTSRARGSVFGQITANHGITLGGDVALNELHRDTRSADRATGEN
                     AWFNEFTSEPWRIGIYLQDNIEYDGLIADVGIRVDGFNANWPVYFPEDEYPEAFARGG
                     MRAISEQHGIPVDMAALGIVSPNSSPELLRYIDAPLSTDPGWIHSERLLAVLPSEKAQ
                     THWRVSPRFGVSHPAGDRARVFFNYGRMHSAPAAGYRYGFLAKRQRIGGFEGEIVGVG
                     NPNLEPPRTTLYEVGADYRVLSQVAFRVRFYSGSETKEPTSYIKVGGVQSVMTGGVNT
                     SEFRFASVSNDGYRLFRGIEASIEKPLGSFITGSVNIDLREVTIGHTGVLEDYEDPSR
                     SDVAADVADVCTDFRPTVQGLLMLSTPTVWGSLLGGWELSATQIYRRGNTEVYHRAAD
                     GLASNSNGNTVQSIVFRWVDTWRTNARASKTIRIGEGSFTIYGDVRNVFNVKTFNLAA
                     VDRVTYFEDILMEGGDVPLHRIGDTSYSIDGENVDDIVKRRLVRENDWLLYLYPRSFQ
                     FGVRVNL"
     gene            1633..3978
                     /locus_tag="ENN56_02840"
     CDS             1633..3978
                     /locus_tag="ENN56_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74454.1"
                     /translation="MARLAFASTACFAFLASAQGASAQGLSAPLPEWRPSLNGWSTAS
                     GEALAPVDGSAVPNSDLTDRISLRVGRLWHTLDASASTGANASWAWPGGIVRPFSSEP
                     SAERFGSNSSLQGVVVGARNWVTVTPEQQVAYTYVTNEAYRFVADGYHQKFTGKPTEI
                     SVYGRLEAAQVQRWLPPQVIVDGVPIHPFTDSYGSNPGSPPAGDGAMRFGAVRGVIDP
                     TLRSERMAYTAWRFPIGVDVIQQVHAWGSSPDNDYLLYDVNIVNTGNTDSDPTAERGG
                     SLEDFRYAFAFGVDVNRTGNLFDGGADDIVEFVNPWPGYSVNGVEKVAALMYDGDSRS
                     RFGPDWGAPGGGYLDGVDIPIQPRLHAPAALLVGWLFAEHAPGSGIDDPAQPKTTTWS
                     NEDAYRITSLENDLRDQYQRIFAAKDFDPMFAPSADTVTYRLPVGVTQSDFGVSGRNP
                     SVYQTLAYEDLAHGESVRAILVVAAGGVNDSVSRAIGHAVVTRDADRSVFANDRMTAT
                     EIALVRSNRDSAITTAQRAFWNVYGNDPNGGKWTPKPAVQNTTFNVPDPPKPPAAVWI
                     TSGNGYIDLAWTTDASTVPDTDLGTYDFAGYRVYRSVGSTTSPRKVIAELPADAAGFR
                     DSTVARDVDYYYAVTTFDDGSANWSEPQVSLESSVYWTWSGWSDGAAARAGGSVLTEA
                     PTVDMVRVVPNPYHIDMTGTRGSIRDEISFVNLPSSATIRVYTAGGEFVHEIVHTSPY
                     PASNATWDLRTVANRRIATGVYVYTVDTGDARKVGKFIVVQ"
     gene            complement(4007..>4167)
                     /locus_tag="ENN56_02845"
     CDS             complement(4007..>4167)
                     /locus_tag="ENN56_02845"
                     /inference="COORDINATES: protein motif:HMM:PF00248.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS74455.1"
                     /translation="SVAQIAMAYVIHSPMNVFPIVGAANRTELEANLAAMQTTLTEEE
                     RAWLNLEV"
BASE COUNT          900 a         1063 c         1248 g          956 t
ORIGIN      
        1 ctatctctct ggtggtggat acgtttccga cttttctgaa tggatgactt ctcgagcgcg
       61 tggatcggta ttcggacaaa tcacggcaaa tcacggcatt accctcggtg gtgatgttgc
      121 gctcaacgaa ctccatcgcg atacgcgtag tgccgaccgc gcgaccggcg aaaacgcgtg
      181 gttcaacgag ttcacttcgg aaccttggcg gataggtatc tacctccagg ataacatcga
      241 atacgatggt cttattgccg acgttggcat ccgtgtagac gggttcaacg cgaattggcc
      301 cgtctacttc ccggaagatg agtatccgga agcgtttgca cgcggaggaa tgcgggcaat
      361 tagcgagcaa catggtatac cggtggatat ggcggcgctc ggtattgtat cgccgaatag
      421 ttctcctgag ctgttgcggt acatcgatgc accgctttcg acggacccgg gatggataca
      481 ctcggaacgc ttactcgctg ttcttccgag cgaaaaagcg caaacgcatt ggcgcgtatc
      541 gcctcgattc ggcgtgagtc atccggctgg tgatcgcgcg cgtgtattct tcaactacgg
      601 aaggatgcac agtgcgccgg cagcaggata tcgatacggt ttccttgcga aacgccaacg
      661 aatcggtggc ttcgagggcg aaatcgtcgg cgtagggaat ccgaatctcg aaccaccaag
      721 aactactctg tacgaagttg gcgccgatta ccgcgtgctg agtcaagttg ctttccgggt
      781 acggttctac tcgggaagtg aaacgaaaga accgacatca tacataaaag tcggtggagt
      841 acaatcggtg atgaccggtg gagtaaacac gtcggaattc cgtttcgcgt ccgttagcaa
      901 cgatggttat cgtctgtttc gtggtattga agcgagtatc gaaaagccgt tgggctcatt
      961 tattaccggt tccgtaaaca ttgatctgcg cgaagttacc attggccaca cgggtgtact
     1021 tgaagattac gaggatccat cgcgcagtga cgttgctgct gacgtggcgg acgtgtgtac
     1081 ggatttccgg ccgaccgtgc aaggattgct catgctgtca acacctacgg tttggggatc
     1141 gctcctcggc gggtgggaac tgagcgccac tcagatatat cgacgtggga ataccgaagt
     1201 gtaccatcga gcggcggacg gtcttgcctc gaattcaaat ggtaatacgg tccagtcgat
     1261 cgtatttcgt tgggtggaca cgtggcgcac gaatgcacga gcgagtaaaa ccatccgtat
     1321 cggcgaaggg tcgttcacga tctatggcga cgtacgaaac gtgttcaacg taaaaacgtt
     1381 caatctcgcg gcagtcgatc gcgtcaccta tttcgaggat atcctcatgg aaggtgggga
     1441 tgtccctctg catcgcatcg gtgatacgag ttactcgata gatggtgaga acgtggacga
     1501 cattgtcaaa cgccgtctcg tccgggaaaa cgattggctg ttgtatctct acccgaggtc
     1561 gttccagttc ggtgtccgag tgaacctatg agaacgcatg tgactgttca tacgatgaga
     1621 gcacgtcgct tcgtggcacg tcttgcgttt gcttcgactg cgtgtttcgc gtttctcgcg
     1681 agcgcacagg gtgcgagcgc acagggattg agtgcgccgc ttcccgagtg gcgcccctct
     1741 ttgaacggtt ggtcgacggc gagtggcgaa gcgcttgcac cggtggacgg atcggctgtt
     1801 ccaaactccg acctgacgga tcggatttcg ctacgcgtcg gacgactctg gcatacgctc
     1861 gatgcatccg cgtcgaccgg tgcgaatgca tcttgggcgt ggcctggagg cattgtccga
     1921 ccattttcat cagagcccag cgctgaacgg ttcggatcga atagcagtct gcaaggcgtt
     1981 gttgtcggcg cgcgtaactg ggttaccgtg acacccgagc agcaggtcgc gtatacatac
     2041 gttacgaatg aagcgtaccg gtttgttgcc gatgggtatc atcaaaagtt tacgggtaag
     2101 ccaacggaga tcagtgttta cggtcgactc gaagcagctc aggtgcagcg atggctcccc
     2161 ccgcaggtaa tcgtagatgg tgttccgatt cacccgttta cggactctta cggatcgaat
     2221 ccgggttcgc cgccagccgg agacggcgcg atgaggttcg gagcggtccg cggcgtgatt
     2281 gatcccacgc tgcggagcga acggatggcg tacacggcgt ggcgcttccc gatcggagtt
     2341 gatgtaatcc aacaggtaca cgcatggggg tcttccccgg ataacgatta cctgttgtac
     2401 gacgtgaata tcgtgaacac cggcaacact gattctgatc cgactgctga acgtggcgga
     2461 tctttagagg attttcgata cgcattcgcc tttggcgttg acgtaaatcg gacgggaaac
     2521 ctcttcgacg gcggcgctga cgatatcgtc gaattcgtaa atccgtggcc cggatactcg
     2581 gtaaatggtg tcgaaaaggt tgccgcactc atgtatgacg gtgattcacg atcgcgattc
     2641 ggacctgatt ggggcgcacc gggcggcggt tatctcgatg gtgtcgatat accgattcaa
     2701 ccgcgattgc atgcaccggc ggctttgctt gtcggatggt tgttcgcaga gcatgcacca
     2761 ggcagtggga tcgatgatcc ggcgcagccg aaaacgacga cgtggtcaaa tgaagacgcg
     2821 taccggatta cctcgctcga gaacgatctt cgcgaccaat accagcgaat tttcgcagca
     2881 aaagattttg acccgatgtt cgcgccgagt gctgatacgg tcacgtatcg gttaccggtt
     2941 ggcgtcaccc agtcggattt cggtgtatcg gggaggaatc catcggtata tcagaccctt
     3001 gcgtacgagg atctcgcaca cggcgaatct gtgcgcgcga ttctcgttgt tgccgccggc
     3061 ggggtaaacg actcggtaag tcgcgctatt ggtcatgctg ttgtaacacg ggatgccgac
     3121 cgatcggttt tcgcgaacga ccgtatgaca gccaccgaga tcgcacttgt acgaagtaac
     3181 cgagattcag cgataacgac tgcgcaacgg gcgttctgga acgtctacgg taacgatccg
     3241 aacggtggaa agtggacccc aaagccggcg gtgcagaata caaccttcaa tgttcccgat
     3301 ccaccgaagc ctccggccgc ggtctggatc acgagtggga acgggtacat cgatctcgcg
     3361 tggacaaccg atgcgagtac ggttccagac accgatctcg ggacgtatga tttcgccgga
     3421 taccgagtat atcgttcggt cggatcgact acttcaccac gtaaagtgat agccgagcta
     3481 ccggctgatg cagccggttt ccgtgactcg acggtcgcgc gtgacgtgga ttattactac
     3541 gccgtgacga cctttgacga tggatcggca aactggtcgg aacctcaggt atcgctcgaa
     3601 tcttccgtgt attggacgtg gagtggctgg tcagacggag cagccgcacg tgctggtgga
     3661 tccgttctta cggaagcgcc gaccgtggat atggttcggg tcgttccgaa tccgtaccat
     3721 atcgacatga ccgggacgcg aggaagtatt cgcgatgaaa tctcatttgt aaatctacca
     3781 tcgagtgcaa cgattcgcgt gtatacggcg ggtggagagt tcgtgcacga gatcgtacat
     3841 acgagtccgt atcccgcgag taacgctacg tgggatctcc gaacggtcgc gaatcgacga
     3901 atcgccaccg gtgtctacgt gtatacggta gacaccggcg atgcgcgaaa agtcggcaag
     3961 tttatcgttg tacagtgatt gtgggtcgcg aaatatctca acaatgtcaa acttcaaggt
     4021 tgagccacgc acgttcttct tcggtgagcg tagtttgcat cgcggcgaga tttgcctcga
     4081 gctccgttcg gtttgccgct ccaacgatcg ggaatacgtt catcgggctg tgtatcacat
     4141 aggccatggc gatctgcgcg acggaga
//
LOCUS       DSCC01000229            1516 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055999, whole genome shotgun sequence.
ACCESSION   DSCC01000229 DSCC01000000
VERSION     DSCC01000229.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055999"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            36..584
                     /locus_tag="ENN56_02850"
     CDS             36..584
                     /locus_tag="ENN56_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74456.1"
                     /translation="MQPAVKLLDDGTGRQRMAYVALDDDDQFHLYRQIDAYNLEDVGK
                     LRLETGEPIGANFLGAGGTGRLKLVFADWNGNGVADLIVGTPRHGSVPDPETGLPQSL
                     GLPGAAVLFLENIGSNAEPIFKRPELFKFRGEPIFLGQHACGPTVWYPKPDADNPNPR
                     TGLVVGQEEGRVMFFAREDLSP"
     gene            619..1371
                     /locus_tag="ENN56_02855"
     CDS             619..1371
                     /locus_tag="ENN56_02855"
                     /inference="COORDINATES: protein motif:HMM:PF00326.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS74457.1"
                     /translation="MLKIQRIGAMLPCETDVFEVSGARAFAFLPCSDVRKTPMPWVWY
                     APTFLDNNPGPENVPMFRRWLERGVAIAGIEVGESYGNPTGRAIFTAFHRHLTEKRDF
                     ARKVVLHPQSRGGLMLYNWAVEHPECIAASVGVYTVCDIRSYPGAETAAPAYGMTTEQ
                     FEAVLAEHNPIDRVAPLAAQNVPILHIHGDSDSLVPLEKNSAEFARRYRELGGSMEVI
                     VVPGKGHEAIPEYFEREELMAFGEREAMRRFH"
BASE COUNT          312 a          428 c          473 g          303 t
ORIGIN      
        1 actgcaatgg gctggcggtt cacggaacat ggcgcgtgca acctgccgtg aaactcctcg
       61 atgacggcac cggtcggcag cgtatggcgt atgtcgcgct ggatgacgac gatcaattcc
      121 acctctaccg gcaaatcgac gcctacaacc tcgaagacgt gggaaagctc cgactcgaaa
      181 ccggtgaacc aatcggcgcg aacttcctcg gcgcaggtgg cacaggccgt ctcaagcttg
      241 tgtttgccga ttggaacgga aacggggtcg cggacctcat cgtcggaaca ccgcgtcacg
      301 gctcggtgcc ggatccggaa acggggctcc cgcaatcgct tgggcttccc ggtgccgcgg
      361 tgctcttcct cgagaatatt ggttctaatg ccgaaccgat tttcaaacgt ccggaactct
      421 tcaaattccg cggcgaaccg atcttcctcg gtcagcacgc gtgtggtccg acagtgtggt
      481 acccgaagcc cgatgcagac aatccgaatc cgcgcacagg tctcgtcgtc gggcaggagg
      541 agggccgggt catgtttttc gcgcgcgagg atctgagtcc gtagacgcaa aatctgtcgt
      601 ttgcagaagg aaaatcctgt gctgaagatt cagcgtattg gcgcgatgtt gccgtgtgag
      661 accgacgttt tcgaggtgtc cggagcgcgt gcattcgcgt tcctaccatg ttccgatgtc
      721 cgaaaaacac cgatgccgtg ggtgtggtac gcgccgacgt tcctcgacaa caatccgggt
      781 ccggagaatg tcccgatgtt ccgccgatgg ctggaacggg gtgttgcaat cgctggtatc
      841 gaggttggcg aatcgtacgg gaatcccacc ggtcgggcga tatttacggc gtttcatcgg
      901 catctcacgg aaaaacgtga ttttgcgcgg aaggtcgtgc tgcacccaca gagccgtggc
      961 gggctcatgc tgtacaactg ggcggtagaa catccggagt gtatcgccgc gtctgtcggt
     1021 gtctatacgg tctgcgatat ccgaagctac ccgggtgccg agaccgccgc gcctgcgtac
     1081 gggatgacga cagagcaatt cgaagcggta ctggccgagc acaaccccat cgatcgtgtt
     1141 gctccgctcg cggcgcaaaa cgtgccgatt ctccacatcc acggcgacag cgactcgttg
     1201 gttccgctgg agaaaaacag cgcggagttc gcgcgacgct accgtgaact cggaggatcg
     1261 atggaggtaa tcgtcgttcc ggggaaaggt cacgaggcaa tcccggagta cttcgagcgc
     1321 gaagagttga tggctttcgg cgaacgcgaa gcaatgagac gattccattg agtcgaatcg
     1381 gcggaaccgc ttcgccccat gttttcgagc gttgctgtag ctaccgttga aaaccgcacg
     1441 gggcgaaggc aacgaatgcg caagcattcg cgccttcgcc ttaacgaacc cgtagcgtag
     1501 cgaagggttc gcggtg
//
LOCUS       DSCC01000230            2655 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026252, whole genome shotgun sequence.
ACCESSION   DSCC01000230 DSCC01000000
VERSION     DSCC01000230.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026252"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(209..1693)
                     /gene="dnaB"
                     /locus_tag="ENN56_02860"
     CDS             complement(209..1693)
                     /gene="dnaB"
                     /locus_tag="ENN56_02860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDS74458.1"
                     /translation="MNDAFTGGYPVQQYPDDGPVTDIEEVQRGSGGTRVPPHNVDIEQ
                     QVLGAMLLSRQAISKALAIVDEQAFYRGRHRHIYQAIARLYEAGEPVDLTLLADELEK
                     MGVLEECGGVAYLGDIATATGSSVNVEHHARLLLEKAVRRKTISLASQLVTDSYESGD
                     DIFESLEQFESELFSLAEGRLQTGFRHIEPIMHTAYEKIETVSRHEGEVTGVPSGFQR
                     FDELTAGFQPGELIVIAARPGMGKTAFILNLTRNAAVQYRLPVGIFSLEMSSEAVAQR
                     LLCMEARVDGQSVRTGRLSEPLWNRLSRAVTTLTNAPIFIDDTPAISPLEVRAKARRL
                     VHEHNAQMIIVDYLQLMRVTRRYDNRALEVGEISQSLKALAKDLRIPVIAVSQVTREV
                     EKRGDTRPKLSDLRESGSIEQDADVVGFVHRPEYYKIMQDDEGRSLEGVAEIILEKQR
                     NGPTGKVYLRWFDKYGLFEPLAPDDELGAIAAVQEPDEDDETPF"
     gene            complement(1781..2644)
                     /locus_tag="ENN56_02865"
     CDS             complement(1781..2644)
                     /locus_tag="ENN56_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDS74459.1"
                     /translation="MARPDTTDLARIAREFVEQQRQLGDTELWPKPDPNRVARIRAES
                     TREDAEESETHRPRIARSSEPVQPAHSPVKKPPVVIPMKTPTPQNADTSQPLDPGEWE
                     GLTLDQFEAAIAGCKRCRLHENALNFVFGKGNPNADIMFIGEAPGQDEDRQGVPFVGR
                     AGQLLDKMIVAMKLDPGDVYIGNICKHRPPNNRNPQPDEVEACLPYLIHQIEFVKPKC
                     LCLLGRVAAQALLGSNDSLGRMREKWFEYRGTKVLVTYHPAALLRNPGWKKHAWEDLK
                     RLRKAYDGVIL"
BASE COUNT          525 a          781 c          767 g          582 t
ORIGIN      
        1 gatacgacga acacgagtgg aatcgatctg acaccgactt cggccatctg ttcgagtgtg
       61 agccgcggat tgcggaaaat ctgcggagca atacggagta tggagaacag taagagaaca
      121 atacgtccca cgtgaacgaa gaaatttacc tgtgcgcgac cgatgcgtcg caaggtgtta
      181 cgcacacctg tacggagtcg ttcgaccatc aaaacggtgt ctcgtcgtct tcgtccggtt
      241 cctgcaccgc cgcaatcgcc cccaactcat catccggtgc gagcggctca aacaaaccgt
      301 acttgtcgaa ccagcgcaga tataccttcc ccgtaggacc gtttcgctgc ttctcgagga
      361 tgatctccgc aacaccttcc agcgaccggc cttcgtcgtc ctgcatgatt ttgtagtatt
      421 ccggacgatg cacgaacccc accacgtccg catcctgctc gatgctaccc gattcgcgaa
      481 gatcgctcag cttcggacgg gtgtcgccac gcttttcaac ttcacgagtg acctgcgaca
      541 ccgcgattac cgggatgcgt aaatccttcg caagcgcctt gagcgactgc gagatctcac
      601 caacttccag cgcgcgattg tcataccgcc gcgtgacgcg catgagctgg aggtagtcga
      661 cgatgatcat ctgcgcgttg tgctcgtgca caagtcgacg cgccttcgca cgcacttcca
      721 gcggtgagat ggcgggcgtg tcgtcaataa agatgggcgc gtttgtcagc gtcgtgacgg
      781 cgcgactgag gcggttccac agtggttcac tgagacggcc ggtacgaacg gattgtccat
      841 cgacccgcgc ttccatgcag agaaggcgtt gcgcgaccgc ctccgatgac atttcgagcg
      901 agaagatgcc gaccggaagt cggtattgca cggccgcgtt ccgcgtgagg ttcaggatga
      961 acgccgtttt tcccataccg ggtcgcgcgg caataacgat caattcaccg ggttggaatc
     1021 cggccgtaag ctcgtcgaat ctctggaaac cgctcggtac gcccgtaact tcgccttcat
     1081 gccggctcac cgtctcgatt ttctcgtacg ccgtgtgcat gatcggttcg atgtggcgaa
     1141 accccgtctg gagacggcct tccgcaaggc tgaatagctc cgattcaaac tgctcgagcg
     1201 attcgaagat gtcgtcgcct gactcatagc tgtcggtgac aagctgactc gcgagggaga
     1261 tcgttttccg acgtacggct ttttcaagga gcagccgcgc atggtgctcg acattaaccg
     1321 agctaccggt ggctgttgcg atgtcaccga ggtacgcgac gccgccgcat tcctcgagga
     1381 cacccatttt ctcgagctcg tcggcgagaa gtgtcagatc aactggttca ccagcctcat
     1441 acagccgagc aatcgcttgg tatatgtgtc ggtgccgacc gcggtagaac gcctgctcgt
     1501 cgacaatcgc aagagctttg ctgatcgcct gccgcgaaag caacatcgcg ccaagcacct
     1561 gttgttcgat gtcgacgtta tgcggtggaa cacgagtgcc accgcttccg cgttgaacct
     1621 cttcgatatc ggttacgggc ccgtcatcag gatactgctg cacgggatac cccccggtga
     1681 aagcgtcatt cacaatggga aacggtatta ttcggagtac gtagtgtacg aagatacgat
     1741 gatcggcgac gcaggtcgcc cccagggaaa tgttgcgccg ttacaaaatc acgccgtcgt
     1801 acgctttgcg gaggcgtttg aggtcctccc atgcgtgctt cttccagccg ggattccgga
     1861 gcaaggctgc cgggtggtac gtcacgagca ccttcgttcc gcggtactcg aaccactttt
     1921 cacgcatccg accaaggctg tcattcgagc cgagaagcgc ctgcgcggcg actcgaccga
     1981 ggagacagag gcacttcggc ttgacgaact cgatctggtg gatgaggtac ggcaggcagg
     2041 cttccacctc gtcaggctgc ggattccggt tattcggcgg gcgatgctta cagatattgc
     2101 cgatgtacac atcgccggga tcaagcttca tagcgacgat catcttatcg agaagctgcc
     2161 ccgctctacc gacaaacggc acgccctgtc gatcttcgtc ctgccccggc gcttcgccga
     2221 tgaacatgat atcggcgttc ggattgccct ttccgaacac gaagttcagc gcgttctcgt
     2281 gaagacggca ccgtttgcat ccggcaatgg cagcttcgaa ctgatcgagc gtaagacctt
     2341 cccactcgcc ggggtcaagt ggttgcgatg tatcggcgtt ttgtggcgtc ggtgttttca
     2401 taggaatcac taccggaggc tttttgacgg gtgaatgggc cggttgaacg ggttcgctac
     2461 tccgcgcgat acgcggacgg tgcgtttccg attcctcggc gtcttcacgt gtggattccg
     2521 cgcgaattcg tgcgacgcgg ttcggatcag gtttcggcca cagctccgtg tcgccaagtt
     2581 ggcgctgttg ttcgacgaat tcgcgcgcga tacgggcgag atccgtggta tcggggcgcg
     2641 ccatcggttt acgcc
//
LOCUS       DSCC01000231            2069 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036770, whole genome shotgun sequence.
ACCESSION   DSCC01000231 DSCC01000000
VERSION     DSCC01000231.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036770"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..>2069
                     /locus_tag="ENN56_02870"
     CDS             <1..>2069
                     /locus_tag="ENN56_02870"
                     /inference="COORDINATES: protein motif:HMM:PF04389.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDS74460.1"
                     /translation="STFLDPSGVELGAYGAEIGARVDSARFVEIVKSLTAENGSRAFG
                     MPGNVDATEYIARTFTELGFEHVVRQQMSLPVARHHYAYAVLPRPPTSSEGDSAPGDT
                     IHLHSLIPNFARTNTITPGGISGRLVYTKRGYEREFNGHDMAGSILLAEGTVEDRWKL
                     AVTLGSRAAIFIESDDPYTPSGGIFQTSVNFPRFWVTKDVGEKLKEWAEANPDTPIEL
                     HARQPWEYISGANVLGFLPGSDPVLSSEVVILTASFDATSPVVDFAPGAEAAVSPAAL
                     LEMAHLIAEQPNSRSVYFAALNGQHSGTMLGGRTFVQALKRDFPAALRRASELLRKDI
                     RLISDPRLAGKFPEYKQEASQLEALLNTVLANEVAEAERRWQNLVRARQNYRQNAAVT
                     DSRRGADLYEARRAGADELNQAAAEFIPAYHAAVEALIPVIDAFGRSHARFIELLEHD
                     RDALLPIVAAGEDKIRTSIEERLAAARLSDIGKAYNKVARIGPNDHLQTLANERAQLK
                     VVEPILGRYPALFIDLSLTSNSDDVGVFYKGYFYNQQPDLELKRVYGPLGKRFNGYAT
                     NLTIVNDLTGLWSMSDDSIRSLIWSKVNAISKRRDEYRAVEPLVTSGFDETYHYAAHH
                     GFVNATAWLWALLIIGFFVWRMWVLRAKHGTRPPISYWIGIGAGLFVFVLAVTGHIDW
                     RASTRVY"
BASE COUNT          417 a          636 c          605 g          411 t
ORIGIN      
        1 atccacattt ctcgatccga gcggtgtcga actcggtgcg tacggagcgg aaattggcgc
       61 gcgcgtcgat tctgcgcggt tcgtcgagat cgtgaagtcg cttaccgccg agaacggcag
      121 ccgtgcgttc ggaatgccgg gtaacgtcga cgccacggaa tacatcgccc gcacattcac
      181 cgaactcggc ttcgaacatg ttgttcggca gcagatgagc ctccccgtcg cgcgacacca
      241 ctacgcatat gccgtccttc cccgtccacc gacctcatcc gaaggcgatt cggcaccggg
      301 cgataccatt cacctccaca gcttgatacc gaatttcgct cggacgaata ccataacgcc
      361 gggcggtatc agcgggcgtc tcgtctatac caagcgcggg tacgagcggg aattcaacgg
      421 tcacgacatg gccggcagta tcctgcttgc cgaaggaacg gttgaagacc ggtggaaact
      481 tgccgtgacg cttggcagcc gtgcggccat atttatcgag tccgatgatc catatacccc
      541 atctggaggg atattccaga cgtcggtaaa cttcccgcgt ttctgggtga cgaaagatgt
      601 cggcgagaag ctcaaggagt gggccgaagc gaatccggat actcccatcg agctgcacgc
      661 tcggcagccg tgggaatata tcagcggcgc gaacgttctc ggattcctcc ccggctccga
      721 tccggtgctg agttccgagg tcgtcatcct caccgcttca ttcgacgcga cgtcgcccgt
      781 ggtcgatttc gcgcccgggg ccgaagcggc cgtgagtccg gccgcgttgc ttgaaatggc
      841 gcaccttatc gctgaacaac cgaattcgcg atcggtgtat ttcgccgcgc tcaacggtca
      901 gcacagcgga acgatgctcg gcggacgcac gttcgtacag gcgctcaagc gcgatttccc
      961 tgccgcattg cgtcgtgcgt cggaattact tcgcaaggac atccgtctca tcagcgatcc
     1021 gcgtctcgcc gggaagttcc ccgaatacaa gcaagaggcg tcgcaactcg aagcgctgct
     1081 caacaccgtc ctcgcgaacg aagtcgccga agccgaacgg cggtggcaaa acctcgtgcg
     1141 tgcccggcag aattaccgtc aaaacgcggc cgtgaccgat tcgcggcgcg gtgcggatct
     1201 ctatgaggcg cgacgtgccg gtgctgatga actcaatcag gcggcggcgg agttcatccc
     1261 cgcgtaccat gcggccgtgg aagcgcttat accggttatc gacgcattcg ggcggtcgca
     1321 cgcacgcttc attgaattac tcgaacacga tcgcgatgcg ctccttccca tcgtggcggc
     1381 gggcgaggat aagatccgca cgtccatcga ggagcgcctt gcagcggcgc gtctcagcga
     1441 tatcggcaag gcgtacaata aggtcgcgcg cattggtccg aacgatcacc ttcagaccct
     1501 cgccaacgag cgcgcgcagc ttaaggttgt cgaaccgatt ctcggccgat atcccgcgct
     1561 attcatcgat ctctcgctga cgagtaactc cgatgacgtc ggcgtatttt acaaaggcta
     1621 cttctacaat cagcaacccg atctcgaact gaaacgcgta tacggtccac tcggcaagcg
     1681 tttcaacgga tatgcgacga atctgacaat tgtgaacgat ctgacgggct tgtggtcgat
     1741 gtccgacgac agcatccgat cgctcatctg gtcgaaagtg aacgcgatca gcaagcgtcg
     1801 cgacgagtac cgcgccgtgg aaccgctcgt aacatccggc ttcgacgaga cgtaccacta
     1861 cgcggcgcac cacggtttcg tcaatgcgac cgcgtggctc tgggcgctgt tgatcatcgg
     1921 gttcttcgtc tggcggatgt gggtattgcg cgcgaagcac ggaacacgtc cgccgatatc
     1981 gtactggatc ggcatcggtg ccggactctt tgtgttcgta ctcgccgtga ccggtcacat
     2041 tgattggcga gcgagcaccc gcgtgtacc
//
LOCUS       DSCC01000232            5021 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1011107, whole genome shotgun sequence.
ACCESSION   DSCC01000232 DSCC01000000
VERSION     DSCC01000232.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1011107"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..585
                     /locus_tag="ENN56_02875"
     CDS             <1..585
                     /locus_tag="ENN56_02875"
                     /inference="COORDINATES: protein motif:HMM:PF13860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74461.1"
                     /translation="IYGQAIDPSSGEPPVYNAYVQLFDATDKQVGSEYVSLTDGWFLF
                     DDLAPGNYTLRGYIDGTTLVREVDVTIPMAPKEFRLLLGDTNSVHNGGAHPERFAVGN
                     PFPNPANPTVSIEYRIAEAQPGESVQLSILNTLGQTVRSERLHATSTGIITWDGCDGR
                     GELVSTGVYFARLHLADGMGRTEATVTRRITLLR"
     gene            complement(621..1466)
                     /locus_tag="ENN56_02880"
     CDS             complement(621..1466)
                     /locus_tag="ENN56_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015251928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDS74462.1"
                     /translation="MRRNHTASKIAKKTWTYAFLIFSSIVFSAPFLLTVSNSLKDLRE
                     SYAFPVVWIPEDPQWINYVKVFELLPFAHFIWNTVIVTVASLTGLLLSASIVAYAFAR
                     LEWKGRDIWFIVLLSTLMLPHQVTMIPTFLVFKWLGWIDTYKPLVVPLWLGKNVFSIF
                     LLRQFFKTIPVDLEDAALIDGCNKFQIFYRIMLPLAKPALATIAVLSFIAVWNDFFDP
                     LIYLQSYEKFTISLGINMFKDTQSQFPHYLMAATVISLVPVVIIFFAAQKYFVEGIVL
                     TGQKN"
     gene            complement(1466..2299)
                     /locus_tag="ENN56_02885"
     CDS             complement(1466..2299)
                     /locus_tag="ENN56_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDS74463.1"
                     /translation="MVFAIFLSLAKWDAQTSLANVDFVGLQHYKTIFTSDRLFWTSLY
                     NTAFYTAFAVPLRIFGALILAMILNQQIRGVSVFRTLFYLPSVVSGAATAILWWWVFN
                     PYFGALNSLLTPILSIFGAQPPGWLEDPNWSKPALILMTAWGLGAPMLIFLAGLQGVP
                     RHLYEAAELDGAGRLRKFWNVTVPMITPTILFNLVMAIIGSFQVFMMAFIMSEGNRGG
                     PDDSTLFYVLYLYRKAFIDFEMGYASALAWILFVIILFFTLLVLRSSAVWVYYEGERK
                     K"
     gene            2746..>5021
                     /locus_tag="ENN56_02890"
     CDS             2746..>5021
                     /locus_tag="ENN56_02890"
                     /inference="COORDINATES: protein motif:HMM:PF01204.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74464.1"
                     /translation="MPRRPALYPRPRWQWATDLPRVESPWAEFVSLPDVRFLSDNQVS
                     LWIAPDTIGTEKISFYPVPRAPHLDADRYDVGRIMEESVHSMSGPRAEVLRGGEPIAF
                     DAPESTWLPSHVERSFRSGDVVVHETIASYDDMVIYWIRPEGATDDLTIRWAATVDRA
                     TSASWRTHGNAHVCLHDDGVANGFVWLGSDLTSWNSGSNAGCLNFTAPLPADGVLLVL
                     MFGYDQAAVERRLESMIAQISKFGTVNMANDLLGAARQQWEWYFSHMVPGFRGGPEWV
                     ERMYYYFMASHKINLYDIPYEPFVRPYSCPWKTGAVWQWSWNTPMNAIAERWLNDMAW
                     AEAGIELIHENGGALNIGSSLHRLRKPRHFRDVNEYLPAVSNAYKGGVSLPATARQFD
                     WAFIMPHTTPLGVHGVWEVFRRTGDERWLKDQLSDLVDYEKTMSGHDMDGDGLVNYAG
                     MVDEYDYSLRWRTAIAEYKKGSESLTHFTRPLELIDINAQLCMLREDIARAAGLFGDE
                     KLAKRIRRRRDKTINAINEHMWDEESGWYQDIDSETHERTGVLSIAATSALLAGVVPK
                     DRAERMLALLDDPTKFGSPWPVPSVAMDTPDLDPSHITYGGDVLLTSGVWTTVQGLVR
                     YGYTDKAREIIWKTLEMVGANGPSSAYSYNSLTGEPNMSRHMFCSQAAIVLDLMLQYV
                     VGLIPREDDIIEIWPFALPYEEWDYVKFGPYVWRGDVDITITWKRGEGYTVQAGKATF
                     TSPEPRHMWLGLDMQDNL"
BASE COUNT         1179 a         1338 c         1458 g         1046 t
ORIGIN      
        1 atctacggtc aggcgattga tccgtcgtcg ggcgaacctc ccgtatataa cgcgtatgta
       61 cagcttttcg acgctacaga taaacaagtt ggatcggagt acgtctccct cacggacgga
      121 tggtttctgt tcgacgattt ggcacccggt aactacacgc tccgtgggta catcgacggt
      181 acgacgctgg ttcgcgaagt cgacgttact atcccgatgg cgccgaagga gtttcggttg
      241 cttctcggtg atacaaactc ggtgcacaac ggtggcgcgc atccggagcg ctttgcggtc
      301 ggaaatccgt tcccaaatcc ggcaaatccg acggtatcga ttgagtaccg aatcgccgaa
      361 gcgcaaccgg gtgagtcagt tcaattgagt attctgaaca cgcttgggca gacggtgcgg
      421 tcggaacgcc tacacgcgac gtcgaccggt atcatcacgt gggacgggtg tgatggacgc
      481 ggggaactgg tatcgacggg agtgtatttc gcgcgactcc atctcgcgga tggaatgggt
      541 cgcaccgaag cgacggtaac gcgccgtatc acgttactcc ggtaattccg agatctgagt
      601 gtggtcctca tttacttcgg tcagtttttc tgtccggtta gaacgatccc ctcgacgaaa
      661 tatttctgtg ccgcgaagaa gataatcacc acgggcacga gcgaaatcac cgttgccgcc
      721 atgaggtaat gggggaactg cgactgcgtg tccttgaaca tgttgatgcc gagtgagatg
      781 gtaaacttct cgtagctctg gagatagatc agcggatcga agaagtcgtt ccacacggcg
      841 atgaacgaga gcacggcgat tgtcgcgagc gcgggtttcg cgagcggtag cataattcga
      901 tagaaaatct ggaacttatt gcacccgtcg atcagcgctg cgtcttcgag atcgaccgga
      961 atcgttttga agaactgtcg caggaggaag atgctaaaca cgttcttccc aagccacagc
     1021 ggcacaacga gcggcttgta cgtatctatc cagcccagcc acttgaacac gaggaatgtc
     1081 gggatcatgg tcacttggtg aggtagcata agcgtgctga gcaggacgat gaaccaaata
     1141 tcgcgtccct tccactcgag ccgtgcgaac gcgtaggcga cgatggaggc gctcaggagt
     1201 agaccggtga gcgacgctac cgtaacgata accgtattcc agatgaaatg cgcaaacggc
     1261 agtaactcga ataccttgac gtagtttatc cactgcggat cttcgggaat ccagacaacc
     1321 gggaacgcgt aactctcgcg caaatccttc aacgagttgg acacagtcag caaaaacggc
     1381 gcggagaaga caattgacga gaaaataaga aacgcgtacg tccacgtctt tttcgcgatc
     1441 ttcgacgccg tgtgatttcg cctcatcatt tcttccgttc tccctcgtaa tatacccaga
     1501 ccgccgatga gcgcaggacg agtagcgtga agaacagaat gatcacgaac agaatccacg
     1561 cgagcgccga agcgtagccc atttcaaagt caataaacgc tttccggtag aggtagagta
     1621 cgtagaaaag cgtgctgtcg tccggaccgc cgcggttccc ttcggacatg atgaacgcca
     1681 tcatgaacac ctggaaagaa ccgataatcg ccatgacgag attgaacaga atcgtcggtg
     1741 tgatcatcgg gaccgtaacg ttccagaact tccggagtct tccagcaccg tcgagttcgg
     1801 ctgcttcata gaggtgacgc ggaacaccct gaagaccggc gagaaaaatg agcatcggtg
     1861 caccgagccc ccacgcggtc atgagaatga gcgcgggttt tgaccagttg gggtcttcca
     1921 accatccggg cggttgcgcg ccaaagatac tgagaatcgg tgtgagcaaa ctgttcaacg
     1981 cgccaaaata cgggttgaac acccaccacc acaggatcgc cgttgcggca ccactgacga
     2041 cactcggaag gtagaacagt gtgcggaaaa cggatactcc gcgtatctgt tgattgagaa
     2101 tcatcgcgag gattaacgcg ccgaaaatcc ggagaggaac ggcgaaggcg gtataaaacg
     2161 ccgtattgta gagtgatgtc cagaacaaac gatcgctggt aaagatggtt ttgtagtgtt
     2221 gaagacctac gaaatcgacg tttgcaagcg atgtctgcgc gtcccacttg gcgaggctca
     2281 ggaaaatggc aaacaccatc ggcccgaggg taaacgcgat aaacccaatg atccacggag
     2341 acgcaaagag atagccgtcg attgtatcgc gcgtgcttct tttcacggta tctcccgaga
     2401 ggatcagaga ccccgaatcg atgtgattcg gtgccaatgt tatcgtaaga acgccatgtt
     2461 tgtgcgatga taaacgtagg gaaaagatag aaaaccgagc gcgaaaccga aagtctctct
     2521 aaccgttacg agtaccattg actgagagaa cgattcttga cattctcttg taccgagacc
     2581 ggcagcgccc gaatgtgtcc ggtcattacg agagcgccgg ctgcaccctt gcggcggaac
     2641 ctccgtcgag ccagcgtcgc accgacccgg tatgtatgca gccggtactg gacaacggac
     2701 ctcttactcg tgacatccgc cttttttccg acggagtagg agaatatgcc acgtcgccca
     2761 gcactgtacc cacgaccgcg gtggcaatgg gcaaccgacc tgccacgcgt agagtcgccg
     2821 tgggctgagt tcgtcagcct tccggacgta cgatttcttt ccgacaatca ggtcagtctc
     2881 tggatcgcac cggatacaat cggcacggaa aaaatcagct tctacccggt tccgcgcgct
     2941 ccccatcttg acgctgaccg atacgacgtc ggacgcatca tggaagagtc cgttcactcg
     3001 atgtcgggac cccgtgcaga ggtcttacgc ggcggtgagc cgatcgcgtt tgacgcgccc
     3061 gaatcgacgt ggcttccttc gcatgtcgag cgctccttcc gttccggtga cgtagtcgtt
     3121 cacgaaacaa tcgcctcgta cgatgacatg gtgatctact ggattcgtcc ggaaggcgca
     3181 acggacgatc tcacgattcg ttgggcggcg acggttgatc gggctacgag cgcgtcatgg
     3241 cgcacacatg gtaacgcgca cgtctgtctc cacgacgacg gggtcgcgaa tggcttcgtc
     3301 tggctcggct cggacctaac atcgtggaac agcggctcca atgccggctg tctgaacttc
     3361 actgcaccgc tgccggccga tggtgtctta cttgttctca tgtttggcta cgatcaggcg
     3421 gcggttgaac ggcgtctcga atcgatgatc gcacagatca gcaagttcgg cacagtgaat
     3481 atggcgaacg atctccttgg tgcggctcgc caacagtggg aatggtattt ctcccacatg
     3541 gttccgggat tccgtggcgg accggagtgg gtcgagcgca tgtattacta tttcatggcg
     3601 agccacaaga tcaatctcta tgacattccg tacgaaccat tcgtacgacc gtattcatgt
     3661 ccatggaaga ccggcgcggt ctggcagtgg tcgtggaata cgccgatgaa cgccattgcg
     3721 gagcgttggc tgaacgatat ggcatgggcg gaagccggta tcgagctaat tcatgagaac
     3781 ggcggcgcac taaatatcgg atcgtcgctc catcggctcc gcaaacctcg gcatttccga
     3841 gatgtgaacg agtatcttcc cgcggtaagt aacgcgtaca agggtggagt gtcgttaccg
     3901 gctactgccc gacaattcga ctgggcgttc atcatgccgc acacgacgcc gctcggcgtt
     3961 cacggcgtct gggaagtgtt ccgtcgcacg ggtgacgaac gctggctgaa ggatcagctc
     4021 agcgatctcg tcgattacga aaagacgatg agcggccatg acatggatgg cgatgggctc
     4081 gtcaattatg ccggcatggt cgatgaatac gattattcac tccgttggcg aacggccatt
     4141 gccgagtaca aaaaggggag tgagtcgctt acgcatttca ctcgaccgct ggagctcatc
     4201 gacatcaacg cacagctctg catgctccgg gaagatattg cgcgcgcggc gggtttattt
     4261 ggcgacgaaa aactcgcaaa acgtatccgc agacgtcgcg acaagacgat caacgcgatc
     4321 aacgagcata tgtgggacga ggagagtggg tggtatcaag atatcgactc ggagacccac
     4381 gaacgtacag gtgttctcag tatcgcagcg acgagcgcgc tgcttgcggg cgtcgtcccc
     4441 aaagaccgcg ccgagcgaat gctcgcgttg cttgacgacc cgacgaagtt cggttcgccg
     4501 tggccggttc catcggtagc catggatacg cccgatctcg atccgtcgca catcacgtat
     4561 ggcggtgacg ttcttttgac gagcggcgtc tggacgacgg tacagggtct tgtgcggtac
     4621 gggtacaccg acaaagcacg tgagattatc tggaagacat tggagatggt cggcgcgaac
     4681 ggaccgtcgt cggcgtactc gtacaattcg ctgacgggtg agccgaacat gtcgcggcac
     4741 atgttctgta gtcaggccgc gatcgtcttg gatctcatgc tgcaatatgt cgtcggactg
     4801 ataccgcgtg aagacgatat catcgagata tggccgttcg cgcttccgta cgaggaatgg
     4861 gattacgtca agttcggacc atacgtctgg cgtggcgacg ttgacatcac catcacgtgg
     4921 aaacgcggcg aaggctacac cgtgcaagcg ggaaaggcga cgtttacgtc gccagagccg
     4981 cgtcacatgt ggcttggcct cgacatgcag gacaatctca t
//
LOCUS       DSCC01000233            1738 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046572, whole genome shotgun sequence.
ACCESSION   DSCC01000233 DSCC01000000
VERSION     DSCC01000233.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046572"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..866)
                     /locus_tag="ENN56_02895"
     CDS             complement(<1..866)
                     /locus_tag="ENN56_02895"
                     /inference="COORDINATES: protein motif:HMM:PF01435.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HDS74465.1"
                     /translation="MIPLTKVRLALLMTATILVAFACAYSTNPVTGKKEFFAYSWQQE
                     IEIGTDADQGIAQEYGIYDDDEIRQYVDRIGQKVLQNSHLRREDAGRQFQDTEFTFRV
                     LDSPIVNAFALPGGYIYVTRGLLAHVENEAQLGVVLGHEIGHVAARHSSQRAFKAQLG
                     QIGLIGGAMLGQAVFDISADEILQLGSSAMQYLFLSYSRDDEREADRLGVEYASKSGY
                     SAGEASRFFRSLTRIQQKTGATIPSWQSTHPDPGDREEAVVQLADTWRTQGYDMSLVN
                     RDPFYNVLDRVVV"
     gene            complement(923..1600)
                     /gene="rsmG"
                     /locus_tag="ENN56_02900"
     CDS             complement(923..1600)
                     /gene="rsmG"
                     /locus_tag="ENN56_02900"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDS74466.1"
                     /translation="MSDSDANGAIVQRWSRNTLDALRAGGIRLSAETLSQLEQYANHV
                     ELRGKSLNLVSASDVTRILDRHVFDSLTALPLVVWEDCDVVDIGSGAGFPGIPLAITQ
                     PSARFLLVERTRKRVAFLMQATRELGLSNTIAVWADAETLALKRGAFADTVTIRAVTS
                     TNEALALAAPLLKPTGVVILWQTADQYEREPTPTGWTAKWQAVLSLDSIERGIRVCHR
                     TVSNSAE"
     gene            complement(1551..>1738)
                     /locus_tag="ENN56_02905"
     CDS             complement(1551..>1738)
                     /locus_tag="ENN56_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74467.1"
                     /translation="FDYTAVPALSSEAKEKLRVIRPTTFGQASRVPGITPADLSVIAI
                     ALERQRRERSNRATVES"
BASE COUNT          377 a          470 c          488 g          403 t
ORIGIN      
        1 acgacaaccc ggtcaagcac gttgtaaaat gggtctctat tgacgagcga catatcgtac
       61 ccttgcgttc gccatgtgtc tgcaagctga acgacggctt cctcgcgatc acctgggtca
      121 ggatgggtcg attgccaaga cgggatggtt gcgcccgtct tttgctgtat gcgtgtgagt
      181 gatcggaaga aacgagaagc ctctccggct gaatacccgg atttcgacgc gtactcgaca
      241 ccaagccgat cagcttcgcg ctcatcatcg cgactgtagc tcaggaagag atactgcata
      301 gcagagctac caagctgaag tatctcgtcg gcggatatgt cgaataccgc ctgcccaagc
      361 attgcaccac cgatcaaacc gatctgcccc aattgcgcct tgaaggcacg ttgtgatgaa
      421 tgacgcgcag caacgtggcc gatttcgtgg ccaagcacga caccaagttg cgcttcgttc
      481 tcgacatggg caagcagacc acgagtgaca tagatgtatc caccgggtag tgcgaacgcg
      541 tttacgatgg gactatcgag gacacggaag gtgaattccg tgtcttggaa ctgacgtccg
      601 gcgtcttcgc ggcgaagatg gctgttctgg agtaccttct gcccgatgcg atctacgtac
      661 tgtcggatct catcgtcgtc atagattcca tattcctgtg caataccttg atccgcgtcg
      721 gttccgattt cgatttcctg ctgccatgaa tacgcgaaaa actctttctt ccccgtgacg
      781 ggattcgtgc tgtacgcgca ggcgaatgcg acgaggatcg tcgcagtcat aaggagcgcc
      841 agtcgaacct tcgtaagtgg tatcatcgcg tcctctcgtt tttcgatttc cgtaaatgcg
      901 attcatgcaa tgaatatatc cgttattcgg ccgaattcga tacagttcga tgacaaacgc
      961 gtatgccgcg ttcgattgag tccagtgaaa gaaccgcttg ccattttgca gtccatccgg
     1021 tcggtgtcgg ttcgcgttcg tactgatctg ctgtttgcca gaggattact actccggttg
     1081 gcttcaggag cggtgctgcg agcgcgagcg cttcgtttgt cgatgtaacg gcgcgaatcg
     1141 tgacagtatc ggcaaacgct ccacgtttaa gggcgagagt ctcagcgtcg gcccatactg
     1201 cgatcgtgtt tgagagcccg agttcgcgag tcgcttgcat gagaaacgcg acgcgtttcc
     1261 gagttcgttc gacgagaaga aaacgtgcag atggctgcgt gatcgcaagc ggaataccgg
     1321 ggaagccggc accgctcccg atatcgacga catcgcaatc ttcccaaacg acgagcggaa
     1381 gagcagtgag gctatcgaat acatgccggt caagtattcg cgtcacatca cttgccgaga
     1441 cgagattgag cgatttacca cgaagttcca cgtggttggc gtactgttca agctgggata
     1501 gcgtctcggc gctgagtcga ataccaccgg cgcggagcgc atcgagtgtg ttacgactcc
     1561 accgttgcac gattgctccg ttcgcgtcgc tgtcgctcaa gtgcgatggc gatgacgctc
     1621 aaatccgccg gcgtgatacc cggcacgcga gacgcctgcc cgaacgttgt cggacgtatg
     1681 actctcagct tctctttcgc ctctgacgag agagcgggca cggctgtata gtcaaaat
//
LOCUS       DSCC01000234            1710 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047655, whole genome shotgun sequence.
ACCESSION   DSCC01000234 DSCC01000000
VERSION     DSCC01000234.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047655"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..359)
                     /locus_tag="ENN56_02910"
     CDS             complement(<1..359)
                     /locus_tag="ENN56_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDS74468.1"
                     /translation="MMRTLSALFRLMRPHQWLKNVFVFAGLAFGERVTSTGELQHVLS
                     IADPIDILRSTLIAFIAFCLVSGAVYVFNDLKDVEQDRIHPLKRNRPLAAGEVSPVFA
                     AIFGIALLAGGLVLAYWL"
     gene            468..935
                     /locus_tag="ENN56_02915"
     CDS             468..935
                     /locus_tag="ENN56_02915"
                     /inference="COORDINATES: protein motif:HMM:PF01316.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDS74469.1"
                     /translation="MSLNRKIIRQRELLKIVRSQAVRTQVELADFLNKRGFAVSQPTL
                     SKDIAELGLVKVPLPDGGYRYQATRKGGPDPQGRRLQISFREFLVKTEPAGQLIVLKS
                     IAGHAAGLAWALDNADWPEVVGTIAGEDTVLIITRSPNDAQIVMSHIEQVLTP"
     gene            932..>1710
                     /locus_tag="ENN56_02920"
     CDS             932..>1710
                     /locus_tag="ENN56_02920"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:PRK00436.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDS74470.1"
                     /translation="MKKTVTVTVFGASGYTGAGIYALLMRHPNVEVVAVSAERNAGEL
                     LSTIFPRLTGVGGDRMLIPADEATQVDADIAFLCLPHEAAMSVAPRLIENGRIVIDLS
                     ADFRMDSVESYKQWYKVDHLAPDLLATKAYGLPELNRANIAAAKLVACAGCYPTSSIL
                     PIAPLLEAGVIDPMRIIVDTKSGVSGAGRKLSQRTHFVETNENFAPYNVGRAHRHVGE
                     IDQELSRAAGQPVRVIFSPHLVPMNQGILSTIYVDLLDQSIT"
BASE COUNT          393 a          507 c          480 g          330 t
ORIGIN      
        1 agccaatacg cgagcacaag tccgccagcg agcaacgcga ttccaaaaat cgccgcgaat
       61 accggcgata cctctccggc ggcgagcgga cggtttcgtt tcagcggatg gattcgatcc
      121 tgctcgacgt ctttcagatc gttgaagacg tataccgcgc ccgaaacaag gcagaatgcg
      181 ataaaggcga tcagcgtaga acggagaata tcaatcggat cggcgatgct aaggacgtgc
      241 tgcaattcgc cggtggaagt gacgcgctca ccgaacgcaa gtcccgcgaa gacgaagaca
      301 tttttcagcc attggtgcgg gcgcatgagc cgaaaaagcg cgctgagagt tcgcatcata
      361 ccggatcgat cctgattctc gacgcgcacg gttgacgccg gaatacacgt aaactatact
      421 aatatacttt cgtcttcagt aataaacatt cacggagcgg tgacgcgatg agcctgaatc
      481 ggaaaatcat ccgacaacgc gaactgctaa aaatcgtccg gtcgcaggcg gttcgcacgc
      541 aggtcgaact ggcggacttc ctgaataagc gtgggttcgc agtgagccag ccgacgcttt
      601 cgaaagatat cgctgaattg ggcttggtca aagtaccgtt acccgatggc gggtatcgct
      661 accaggccac gcgaaagggc ggaccggacc cgcagggtcg ccgtttgcag atttcgtttc
      721 gcgagttttt agtaaagacc gagcccgccg gacagctcat cgtgctcaag tcgattgcgg
      781 gtcatgccgc cggtcttgca tgggcgctcg ataacgccga ctggccggaa gtcgtcggga
      841 caatcgccgg cgaagacacc gtactcatca tcactcgatc accgaacgac gctcagatcg
      901 tgatgtctca catcgaacag gttctcactc catgaagaaa acggttaccg taacggtatt
      961 cggcgcgtcg ggatacaccg gcgcgggtat ctacgcgtta ctcatgcggc atcccaatgt
     1021 ggaagtcgtc gcggtctctg ccgagcggaa cgcgggagag cttctctcga caatattccc
     1081 gcggctgacc ggcgtcggtg gcgaccgtat gctcattccc gccgacgaag ccacgcaagt
     1141 agacgcggat atcgccttcc tctgccttcc gcacgaagcc gcgatgtccg tcgcaccgcg
     1201 tttgattgag aacggacgaa tcgttatcga tctttccgcc gactttcgga tggacagtgt
     1261 cgagtcgtac aaacagtggt acaaagtcga ccacctggcg cccgatctcc tcgcaacgaa
     1321 agcgtacggg ctccccgagt tgaaccgcgc aaacatcgcc gcagcgaagc tcgtcgcctg
     1381 cgcggggtgc tatccgacct cgtccatact gccgatcgcg ccattgcttg aagccggcgt
     1441 catcgacccg atgcgcatca tcgtcgatac gaaatccggc gtctcaggtg cgggacgcaa
     1501 gctcagtcag cgcacgcact tcgttgagac gaacgagaac ttcgcaccct acaacgtcgg
     1561 tcgcgcacac cgccacgtcg gcgaaatcga ccaggaacta agtcgcgcgg ccgggcaacc
     1621 ggtacgcgtg atatttagtc cgcacctcgt cccgatgaat caagggattc tctcgacgat
     1681 ctacgtggat ttactcgacc agtctatcac
//
LOCUS       DSCC01000235            2291 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031967, whole genome shotgun sequence.
ACCESSION   DSCC01000235 DSCC01000000
VERSION     DSCC01000235.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031967"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..307
                     /locus_tag="ENN56_02925"
     CDS             <1..307
                     /locus_tag="ENN56_02925"
                     /inference="COORDINATES: protein motif:HMM:PF04587.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74471.1"
                     /translation="ESIRQGLLTGATAAAIRALTGRPARCDEIRPLPEIALRDMSRAA
                     MERIGAAIGASREFVETGIGTYHGRNVIMVPTRIVDNPVRTVGLGDTISSASWLAEA"
     gene            complement(390..1538)
                     /locus_tag="ENN56_02930"
     CDS             complement(390..1538)
                     /locus_tag="ENN56_02930"
                     /inference="COORDINATES: protein motif:HMM:PF02894.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74472.1"
                     /translation="MEKVKVAVIGTGGMGTGHAEGITKNVEETELVAVCDFDKKVADE
                     KAKTYGVPAFYDHKSLIKAGIADAVTIATPHYFHPPIAIDAFKAGLHVLSEKPIGVTV
                     KAADKMLSAAEKSGLVFGVMYQQRTRGVMQAMKRLVDSGKLGRIYRATMIETHFRTQA
                     YYDSAGWRATWKGEGGGVLLNQAPHGIDIFTWLMGLPEKLQAVTRTWRHNIEVEDEAS
                     ALIWWANGATGYYHTSTAEAPGADVMEIAGDKGKIIFRNGTLTFYEIEPDVDTFCNDA
                     GIMWGSPEAKETPVEIEDRPTGHSEIIRNWARAILYGEELLSPGAEGIRSLEFINAVI
                     LSGATGKPVEFPVPRERYERFIKGKIASSTFVKTVKEKRETDPKHVGK"
     gene            complement(1581..>2291)
                     /locus_tag="ENN56_02935"
     CDS             complement(1581..>2291)
                     /locus_tag="ENN56_02935"
                     /inference="COORDINATES: protein motif:HMM:PF01522.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74473.1"
                     /translation="AAVSLTFDDGVASQYETVIPMLNKRGFHGTVYIPMAEGGRFYAA
                     PDKWRDAVADGHEIGNHSMTHPCSRNLWVAGKKLNLDDMTLDDIEAQIVEAKHRIEDA
                     LPDQPEHSYAYPCGETQVGRGANKQSYIPVVAKHYIAARALGDGISINDPAEVDLCSL
                     WAPMPIGETAERLINIAERAYNGKKWAIIAFHGVGDNHLSNSVEAFGSLLDWLAERRE
                     EVWTDSLIHVARWVHDRQ"
BASE COUNT          402 a          717 c          666 g          506 t
ORIGIN      
        1 cgagtcgatt cgacaggggc tcctcaccgg ggcgacggcg gcggcgatac gcgcgctcac
       61 cggtcgtcct gcgcgctgcg acgaaatccg cccgctaccg gagatcgctc tccgcgacat
      121 gagtcgggcc gcgatggagc gcatcggcgc ggcaatcggt gcttcgaggg agttcgtcga
      181 gacgggtatc ggcacctatc acggtcgcaa cgtgatcatg gtgccgacgc ggatcgtcga
      241 taatccggtg cgaacggtag gcttgggcga tacgatttcg tccgcatcgt ggctcgcgga
      301 agcgtaacac tcgcacgggc atgtacgcag aaagcggggc gaacacgaaa gttcgccccg
      361 cttttcgttt tacaacgcag gaagaacgat tatttgccga cgtgcttcgg atcggtctcg
      421 cgcttttctt tgaccgtctt cacgaacgtg ctcgatgcga tctttccctt gatgaaacgc
      481 tcgtaccgtt cgcgcggaac cgggaactcg accggcttgc ccgtcgcgcc ggagaggatc
      541 acggcattga tgaattcgag gctacggatt ccttccgcgc ccggcgagag cagctcttca
      601 ccgtagagga tcgcacgcgc ccagttgcgg atgatctcac tgtgacctgt gggacggtcc
      661 tcaatctcga ccggcgtctc tttagcttcc ggcgatcccc acatgatacc ggcgtcattg
      721 cagaacgtgt cgacatccgg ctcgatttcg tagaacgtga gcgtcccgtt ccggaagatg
      781 atcttccctt tgtcgccggc gatttccatc acgtctgcgc cgggagcttc cgcagtactc
      841 gtgtgatagt aaccggttgc gccgttcgcc caccagatga gcgcgctcgc ttcgtcttcg
      901 acttcgatat tgtgccgcca cgtgcgagtg accgcctgta acttctccgg tagacccatg
      961 agccacgtga agatatcgat gccgtgcgga gcctgattca gcaatacacc gccaccttcg
     1021 cccttccacg ttgcgcgcca accggccgaa tcgtagtacg cctgcgtgcg gaagtgcgtc
     1081 tcgatcatcg tcgcacgata gatgcgaccc agcttgccgg agtcgacgag ccgcttcatc
     1141 gcctgcatca cgccgcgagt ccgctgttga tacatcacac cgaacacgag cccgctcttc
     1201 tcggcggcac tgagcatctt gtcggcggcc ttcaccgtga cgccgatcgg cttttcgctg
     1261 aggacgtgga gtcccgcctt gaacgcgtcg atggcgatcg gcgggtgaaa gtagtgcggc
     1321 gtcgcgatgg tgaccgcgtc ggcgatgccc gccttgataa gcgacttgtg atcgtagaac
     1381 gccggtacac cgtaggtctt cgccttctcg tccgcgactt tcttgtcgaa atcgcacacc
     1441 gcgacgagct cagtctcttc gacgttcttc gtgatgcctt ctgcgtggcc cgtacccatc
     1501 ccgccggtac cgatgacagc taccttgacc ttttccattg tgagactccc taatgctttt
     1561 ctgttgctcc gttctcgacg ttactgccgg tcgtgtaccc agcgtgcaac atgaatgaga
     1621 ctgtcggtcc aaacttcctc gcgacgttcg gcgagccaat cgagtaggct gccaaatgct
     1681 tcgacggaat tactcaaatg gttatccccg acaccgtgga acgcgatgat cgcccatttc
     1741 ttcccattat acgcccgctc agcgatgttg atcaaccgct cggcggtttc cccgatgggc
     1801 atcggcgccc agagcgagca gaggtcgacc tccgccggat cgttgatcga gattccgtcc
     1861 ccaagtgcac gcgcggcgat gtagtgtttc gcgacaacgg ggatatagct ctgcttattc
     1921 gcaccgcgtc cgacctgcgt ttccccacac ggatacgcgt acgaatgctc cggttgatcg
     1981 ggcagcgcgt cttcgattcg atgcttcgcc tcgacgatct gcgcttcaat gtcgtccagc
     2041 gtcatgtcgt ccagattcag cttcttgccg gcgacccaaa ggttccgcga acacgggtgc
     2101 gtcatgctgt ggttgccgat ctcgtgaccg tcggcgacgg catcgcgcca cttgtccggg
     2161 gcggcgtaga accgaccgcc ttcagccatg gggatataga cggtgccgtg gaatccacgc
     2221 ttgttgagca ttgggatgac ggtttcgtac tgcgaggcga cgccgtcgtc aaacgtgagt
     2281 gatacggccg c
//
LOCUS       DSCC01000236            2198 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033869, whole genome shotgun sequence.
ACCESSION   DSCC01000236 DSCC01000000
VERSION     DSCC01000236.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033869"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(84..>2198)
                     /gene="mfd"
                     /locus_tag="ENN56_02940"
     CDS             complement(84..>2198)
                     /gene="mfd"
                     /locus_tag="ENN56_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDS74474.1"
                     /translation="VLNDHELFSRYRGHHRYRKYRGTTSVKDVFALTPGDVVVHEDHG
                     IGIYQGIERLTVDGVTRDLVRLLYRDNDKLFLPVEQVNRIQRFSADDEARPPISKLGG
                     ADWERVRQKTKKGVVKLAKELIELYARRQAQPGFAFAADSTWMREMEAAFIYEETRDQ
                     IATIDQIKGDMERPVPMDRLVCGDVGYGKTEVAIRAAFKAVIDQKQVAVLVPTTILAQ
                     QHFRTFSERLADFPVRIEQLSRFRTKQQIDKALSDLGRGAVDIVIGTHRLLSKDVGFK
                     DLGLVIIDEEHRFGVKHKERLKELRTAVDVVSMTATPIPRTLYLSLTGARDMSVINTP
                     PKDRIPVHTEIIPFDEDRVAEAILREIDRSGQVFFVHNRVQTIEERAAWLTELLPGVR
                     FRIAHGQMSERLLERVMLDFLDRKFDVLITTMIIQSGLDIPNANTIIVDRADWFGLAE
                     LYQLRGRVGRAAHRAFAYLMVPKDRKLNPDARRRLHAIEEFSDLGSGFKIAMRDLEIR
                     GAGNLLGHEQSGHIAAVGYDLYVKLLEEAIRELKGEIVETMPDCEIEIGVSAYLPDDY
                     VTDGEQKMALYRRLAESRSLIDVDEFVDELRDRYGRLPDTARGLVDIMRLKIVGKHLG
                     ARKIIVGKSGRVQILFHADRTLSQLEITQLVQRSSRQMEFKLDTELALEIRLGDEKGI
                     DQADAAVDAIRAMLDTTITKAA"
BASE COUNT          465 a          613 c          562 g          558 t
ORIGIN      
        1 ggacgaaaag aatgccgaga tatccggcac caagcagaag tcttttgcta catggtgttt
       61 tcattacgat tccttcgaaa cacttacgcc gctttggtaa ttgtcgtgtc caacattgct
      121 ctgatcgcat caaccgccgc gtctgcctga tctataccct tttcatctcc aagtcggatt
      181 tcgagagcga gctccgtatc taacttgaat tccatctgtc gcgatgatcg ctgaacaagt
      241 tgcgtgattt cgagttgtga caacgtgcgg tccgcgtgga acaggatttg tacccgtccg
      301 cttttcccaa caatgatctt tcgcgcaccg agatgcttcc caacaatctt caatcgcata
      361 atgtcgacca agccgcgagc ggtatccggt aggcgtccgt atcgatctcg caactcgtcg
      421 acgaactcat cgacgtcaat gagagacctt gactcggcga gtcgccgata gagtgccatt
      481 ttctgttcgc catcggtaac gtaatcgtcg ggaagatacg cactaacccc gatttcgatc
      541 tcgcaatcgg gcatggtttc gacgatttcg cctttcaact cgcgaatggc ttcttcgaga
      601 agcttgacat atagatcgta cccgacagcc gctatgtgcc cgctctgctc gtgtccgaga
      661 aggttgccgg cacctcgtat ctcgagatct cgcatggcaa tcttgaatcc actgccgaga
      721 tcgctgaact cttcaattgc gtggaggcgt cgccgcgcgt cgggatttag ttttcgatcc
      781 tttggtacca tcaagtacgc aaacgcccga tgcgcggctc ttccgactcg tccgcgcagt
      841 tggtaaagct ctgcaaggcc gaaccaatcg gcgcgatcga cgataatcgt attcgcgttc
      901 ggaatatcga gcccggactg gatgatcatg gttgtaatga gcacgtcgaa cttgcgatca
      961 aggaagtcca acataacccg ttcgagcaga cgttcgctca tctgtccatg tgctatacga
     1021 aaccggacac cggggagaag ttccgtgagc cacgcggcgc gctcctcgat cgtctgtacg
     1081 cgattgtgta caaaaaacac ctgacccgac cggtcaatct cgcgtaatat cgcttccgct
     1141 actcggtctt catcgaacgg aataatctcg gtgtgtacgg gaatccggtc ctttggtggc
     1201 gtgttgatga ccgacatatc acgcgcacct gtcaacgaaa gatacagtgt tcgcggtatc
     1261 ggagttgccg tcatgctgac aacgtcgacg gccgttcgca gctctttcaa gcgctccttg
     1321 tgtttcacgc cgaagcggtg ttcttcatcg ataatcacta agccgaggtc tttgaacccg
     1381 acatccttcg acaatagccg atgcgttcca atgacaatat cgactgctcc gcgtccgagg
     1441 tcgctcagcg ctttgtcgat ttgttgtttc gtgcggaacc gagagagctg ctcgatacgt
     1501 acagggaaat cggccagacg ttcgctgaac gttcggaaat gttgttgtgc aaggatggtt
     1561 gtcggaacca gtactgccac ctgcttctgg tcgatgaccg ctttgaatgc ggcgcgaata
     1621 gcaacttccg tttttccgta accaacgtcg ccgcaaacca atcgatccat tggtaccgga
     1681 cgctccatat ctccttttat ctgatcgatc gttgcgattt ggtcgcgagt ttcctcgtag
     1741 atgaacgccg cttccatttc gcgcatccac gtggaatcgg ctgcgaatgc gaatccgggc
     1801 tgcgcttgac gccgcgcgta gagctctatc aactctttcg cgagctttac gacgcccttt
     1861 ttcgtcttct gtctgacgcg ttcccaatcg gcaccaccga gtttactgat tggcggtcgc
     1921 gcttcgtcgt ccgctgaaaa tcgttgaatt cggttgacct gttcgaccgg aaggaaaagc
     1981 ttatcgttgt cccgatagag taatcgaacc agatcgcgcg ttacgccgtc aacggtaagg
     2041 cgttcgatgc cctgatagat tccgatgccg tggtcttcgt ggacaacgac atctccgggg
     2101 gtaagtgcga acacgtcttt caccgacgtc gtgccgcggt atttccggta gcggtgatga
     2161 cctcggtacc gactgaagag ctcgtgatca ttgagaac
//
LOCUS       DSCC01000237            2005 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038351, whole genome shotgun sequence.
ACCESSION   DSCC01000237 DSCC01000000
VERSION     DSCC01000237.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038351"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="ENN56_02945"
     CDS             complement(<1..1276)
                     /locus_tag="ENN56_02945"
                     /inference="COORDINATES: protein motif:HMM:PF05170.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDS74475.1"
                     /translation="MASSGRALKVILIAVGAIAGVFLIALVLLAIFFPSDRVRAVIEE
                     QAATYLERPLTIGDIGLSFWPLGVAVEDVVIGNDLALSDEPLVEVDRAVVSVRLWPLI
                     ARREVTIREISIQSPRIAYVIGEDGTTSVDGLLVEQPEPEPVKVTPSEVDTTAALAGI
                     PFNLIVQEFSISNGSISFIDRQSDMDITVGSIDQTLRLDIVVAKNSLDVSGDFALGDI
                     TLMAAGQGIGPIELSGAYDALVDLGADSVSYVVDLRLAGVPMTSSGSVRTMSTTPDLT
                     LLLQTGRIEIERLLAQLRLPPESQLARMEASGSVTLDAFVHTVIDSTLEEPPLDLDVS
                     LTLDAIRMKHPDMPLAIDEIDGSVHVTSDTIMVGGFRLRAGGSGIDGEAMLVAWMTEE
                     PFLKTAEISGKVILDEVASIVKLPDSTAVGGST"
     gene            complement(1350..1895)
                     /locus_tag="ENN56_02950"
     CDS             complement(1350..1895)
                     /locus_tag="ENN56_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74476.1"
                     /translation="MPLIPHYGVQFLNLYHDRTYLSLFVEGGSTWNWPEISKAKFYRN
                     DFLWDIGAEVRTSLYSFYRLPVMGYVTVARRMKDVPYPFVDHYWSDTQGWVITQPSRY
                     RIYIGFGFGFGGGGHSPVSGAHGYHGAISRPAETAVHAPAMHLDPTMSLDSWAPVSGT
                     PSLNGVLPQSGSLNRIPMSAN"
BASE COUNT          450 a          613 c          549 g          393 t
ORIGIN      
        1 ctgtcgatcc gcccaccgcg gtgctatcgg gaagcttgac gatgcttgcc acttcgtcga
       61 ggatcacctt tcccgagatc tcggcggtct tgaggaaggg ctcctcggtc atccacgcga
      121 cgagcattgc ttcaccgtcg atgccgcttc caccggcacg caaacggaat ccgcccacca
      181 tgatcgtatc cgaagtcacg tgcaccgaac catcgatttc gtcgatcgcc aacggcatgt
      241 ccggatgctt cattcgaatc gcatccaacg tgagagacac gtcaagatcg agcggaggct
      301 cttcaagcgt cgaatcgatc acggtgtgaa cgaacgcatc gagcgtcacc gatccggacg
      361 cttccatccg ggcaagctga ctctcaggcg gtagtcggag ttgcgcgagg agtcgctcga
      421 tctcgatacg tcccgtttgc agcagaagag tcaggtccgg cgtggtggac atggtgcgaa
      481 ccgaaccgct cgatgtcatc gggacgcctg cgagtcgcag atcgacgacg tagctcacag
      541 agtccgcgcc gagatcgaca agcgcatcgt atgcaccgga tagttcgatc ggaccaatgc
      601 cctgtcctgc cgccatgagt gtgatgtcgc caagcgcgaa atcaccggat acgtcgagag
      661 agtttttcgc cacaacaatg tcgagtcgta gcgtctgatc gatcgatccg acggtaatat
      721 ccatatcgga ttgccgatcg ataaacgata tgctgccgtt cgaaatgctg aactcttgta
      781 cgataaggtt gaatggaatc ccggcaagcg cggcggttgt gtcgacttcg cttggggtta
      841 ctttgaccgg ctccggttca ggctgctcaa cgagaagacc atcgacgctc gtcgtaccgt
      901 cctcgccgat cacgtacgcg attcgcggac tctgtatcga aatctcgcga atcgtcacct
      961 cacgacgagc gatcagcggc caaagacgca ccgataccac ggcccgatcc acctcgacca
     1021 gcggttcgtc actcagcgcg agatcgttac cgatcaccac gtcctcgacc gcaacaccga
     1081 gcggccagaa tgaaagtccg atatctccga tcgtcagtgg acgttcgaga tatgtggcgg
     1141 cctgctcttc gattacggcg cgtacacgat cgcttgggaa gaaaatcgcc aagagcacga
     1201 gcgcaatcag aaatactccc gcgatcgcgc caaccgcgat cagaatcacc ttcagtgctc
     1261 gaccggacga agccatgacg aacctcctcg catttaccca acagagaaac gcgtgacgaa
     1321 acccactacc atcgactgtc tgtgtacctc taattcgcgc tcatcggtat ccgattcagc
     1381 gagccgcttt gcggcaaaac accgttgagc gatggcgtgc cgctcaccgg cgcccacgaa
     1441 tcgagcgaca tcgtcggatc gaggtgcatc gcaggtgcgt ggaccgcggt ctcagccggc
     1501 ctgctgattg ccccgtggta tccgtgcgcg ccactgacgg ggctgtgtcc gccgccgcca
     1561 aagccgaatc cgaaaccgat gtagatccgg tatcggctcg gttgcgtaat cacccacccc
     1621 tgcgtatcgc tccaatagtg atcaacgaac gggtacggga cgtccttcat ccgacgcgcg
     1681 acggttacgt atcccatgac gggcaatcgg tagaacgaat agagtgacgt ccgcacttca
     1741 gcaccgatat cccagaggaa gtcgtttctg tagaacttgg cctttgatat ctcgggccaa
     1801 ttccacgtcg aaccgccttc gacgaagagt gacaggtatg ttcggtcgtg gtacagattc
     1861 aggaattgca caccgtaatg cgggatgagc ggcataacat aattcgcccg gaaaaaggcg
     1921 actttgctcc cctcgtgaga gaaataggga tatccgcgca gcgttccgcc acccccaagc
     1981 cgatatcgga gcggccaata cgccc
//
LOCUS       DSCC01000238            1755 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045973, whole genome shotgun sequence.
ACCESSION   DSCC01000238 DSCC01000000
VERSION     DSCC01000238.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045973"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..449)
                     /locus_tag="ENN56_02955"
     CDS             complement(<1..449)
                     /locus_tag="ENN56_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74477.1"
                     /translation="MTSCGYKKLPKKEVNVAIPMNYETRFTWTGDTMRGILSIDGLDD
                     IALGGAEERGVLDPEHMLLGAAEACHFNTFRTIAANSGVDLRAYRSTASGELSLTKGQ
                     GYQFARIVIRPEITVAEADLKRAQRIMEKAHGMCLITRSLNCPCDMEV"
     gene            complement(446..1372)
                     /locus_tag="ENN56_02960"
     CDS             complement(446..1372)
                     /locus_tag="ENN56_02960"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00932.17,HMM:PF13860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74478.1"
                     /translation="MATPLDHFTADRGMDWKTHARIAIVAFGLAICVGSAGATVVINE
                     IMANPRGDENAREYIELLNTGDTQVKLTGWHIGNTSGDDMIIAPPGSFLPVTIPPGGY
                     LLIFDPDYFDGDEPYVNIPSGAGICTIESSGFGRYGIINSRIDTLTLRSPVGVVDRIQ
                     YTAEDVEEGLSLERIDPMADGMDLANWAPSHLYDGSPGAPNYASQSAPASGTLRIGPD
                     VPEMSATFEIEWPEAPVIASVRIYDALGRNVRDLVTGQRQTARVTLIWDGRDDRGNVL
                     QTGRYVVLAEAVSVDTGRRLRLRGTVVLARRR"
BASE COUNT          355 a          546 c          481 g          373 t
ORIGIN      
        1 acttccatat cacatgggca attcagcgaa cgggtgatca agcacatacc gtgcgccttc
       61 tccatgatgc gctgagcgcg tttgagatcc gcctccgcga cggtaatctc cggtcggata
      121 acgatccgcg cgaattgata cccctgccct ttcgtgagcg acaactcgcc acttgccgtc
      181 gaccgatacg cccgaagatc gacaccggaa ttcgcggcga tcgtgcggaa cgtgttgaaa
      241 tgacacgcct ccgcagcacc gagtagcatg tgctccggat caagcacacc gcgttcctcg
      301 gcaccaccga gtgcgatatc gtccagtccg tcaatcgaga gaatgccacg catcgtatca
      361 ccagtccacg tgaatctcgt ttcgtagttc atgggtatcg ccacgtttac ctccttctta
      421 ggcagttttt tgtaaccaca cgatgtcatc gacgccgcgc gagcactacc gttccccgaa
      481 gccgcaaacg cctgcccgta tccactgaga cagcttccgc gagaaccacg tatcggccgg
      541 tttgtagcac gttccctctg tcgtcacgcc cgtcccagat cagcgttacc cgcgcggtct
      601 gacgttgtcc tgtgacgagg tctcgtacgt ttcgtccgag cgcatcgtag atacgtacgc
      661 ttgcgatgac cggcgcttcc ggccattcga tttcgaacgt cgcggacatc tccggaacat
      721 caggaccgat tcgtagagtt ccggacgcag gtgccgactg cgacgcgtaa ttcggcgcac
      781 ccggagagcc gtcgtacaga tgagagggcg cccaattcgc gaggtccatg ccgtccgcca
      841 tcgggtcgat gcgctccaaa gaaagacctt cctcgacgtc ttccgcggtg tattggatac
      901 gatcaacgac acctacgggt gaacggagtg tcagtgtatc gatgcggctg ttgataatgc
      961 cgtaccgtcc gaatccgctg ctctcaatgg tgcatatccc cgcaccggac gggatattta
     1021 cgtacggttc atcaccgtcg aagtagtccg gatcgaagat gagcaggtac ccgccgggtg
     1081 gaatcgtcac agggaggaaa ctacccggtg gggcgatgat catatcgtcg cctgacgtgt
     1141 tcccgatgtg ccacccggtg agcttgacct gcgtatcgcc ggtattcagt agctcaatat
     1201 actcgcgagc gttttcgtca cctctcggat tcgccatgat ctcgttgatg acgaccgttg
     1261 cacccgccga accgacacag attgcgagac cgaacgcaac tatggcaatc ctcgcgtgcg
     1321 tcttccaatc cattccgcgg tcagcagtga aatgatcaag aggagtagcc atggagaggt
     1381 tcccaacgtt accgttcgct cgcgagcttc cgtgatcgat gacagcggaa tcgccgcgag
     1441 aagactatcg gcaagcaccg ccggtgcgta tgatcctccc gtgctccggg cgatggtacg
     1501 taaaacgtcc tcacgctgct gcgtctgttc gtgttcgata ccaacgacgg agatggcgaa
     1561 ctcggtgtcc gcacgtccga tttcaacgcc gttcatcgtc gcgcgggcga ccaccgtatg
     1621 ctcgcccggc ggaagtcccg gaatcgttgc cgtgtaccgc ccgtcgccac gaggttcagc
     1681 ggttgttgtt acggatccgt caccgaccga aatacgtacg tccgcgccct cgagcggtgc
     1741 atagcgttca tcgaa
//
LOCUS       DSCC01000239            1986 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038853, whole genome shotgun sequence.
ACCESSION   DSCC01000239 DSCC01000000
VERSION     DSCC01000239.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038853"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(142..987)
                     /locus_tag="ENN56_02965"
     CDS             complement(142..987)
                     /locus_tag="ENN56_02965"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74479.1"
                     /translation="MITPVMQRIGLSTLTYRTLPLTEALSHVRRHGFLRADIAIIPGR
                     CDHADPLGMDADGRRAWASMVRDSGLNIASLNVGNGPLNDPDGLDTRVEFLERCIDLA
                     DLAGAPVVTINTGSRVPPAQWLDSARRVDEVVSRLARRAADRGVVLSLEAPHVNSLCS
                     TVDEAMRLFEWIHDGRVTLTFDSSHVHCGSDSMRRGLGRMGPWLDHVHLRDARRDDHA
                     LTPGDGDIDFHDLFRGLSGVRYAGSLAIELLFDDEFAGNLDHIERHVERARAHLRDAL
                     RGALV"
     gene            1274..>1986
                     /locus_tag="ENN56_02970"
     CDS             1274..>1986
                     /locus_tag="ENN56_02970"
                     /inference="COORDINATES: protein motif:HMM:PF00701.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDS74480.1"
                     /translation="MKDFTPAYGIVPPLLTPFNEDKSIDWDAYERLIEWHIEKGVSGL
                     FVVCGSSEYWQLTDDESVEMAKFAVKVAGDRINILAGSTKSPDDDLDRNIAVTKRMWE
                     ETGVKGCYLTTPRKLPPEDDIMVEYHMKIHDAVECPVYAYEMPGGTNYKFSPEAFAKI
                     GQGERFIGIKDTTCDLEKVRKKIEAAKGTILIMEANTPNFLESLKLGSVGGINTSANV
                     EPGLFVMLYKLFQKGDLETA"
BASE COUNT          418 a          556 c          605 g          407 t
ORIGIN      
        1 catatttctc ggtaagcgcg cgtgattgat ccttgtcggg atcgtatccg gcgaccaacg
       61 ttgcgttcgg catgtccggg atgtgctgca cgagcgatcc cgccatggag ccgcatccga
      121 tgacggcgca gcgaacttcc ttcatacgag tgctcctctc aacgcgtcgc gcaggtgcgc
      181 gcgcgcacgt tcaacgtgtc tttcaatgtg atcaaggttt cccgcgaact cgtcgtcaaa
      241 gagcagttcg attgcgagac tgcctgcgta ccgaactccc gacaggccgc ggaacaagtc
      301 gtggaagtcg atatcgccgt ctcccggtgt caacgcgtgg tcgtcgcggc gggcatcgcg
      361 caggtgtaca tgatcgagcc acggtcccat acggccgagt ccgcggcgca tggaatcgct
      421 cccgcagtgg acgtggctgg aatcgaacgt cagcgttacg cgtccgtcgt gaatccactc
      481 gaacaggcgc atggcttcgt caaccgtgga gcagagcgag ttcacatgcg gtgcttcgag
      541 gctcaggacg actccgcgat ccgcggcacg tcgtgcgagt cggctgacaa cttcgtcaac
      601 gcggcgcgcg ctatcgagcc actgtgccgg cggaacgcgt gatccggtgt tgatcgtgac
      661 gaccggtgca cctgcaagat cggcgaggtc gatgcagcgt tcgagaaact cgacacgcgt
      721 atcgagtccg tcgggatcgt tcagcggacc gtttccgaca ttcagcgatg cgatgttgag
      781 accggaatcg cggaccatac tcgcccatgc acgccgtccg tcggcatcca ttccgagcgg
      841 atcggcatga tcgcatcgac cgggtatgat cgcgatgtcc gcgcggagaa atccatggcg
      901 tcggacgtgg ctaagcgctt ccgtgagggg aagcgtgcga tacgtaagcg tggaaagacc
      961 gattcgctgc atgaccggcg taatcatcgg tagctacctt cgatgtgaaa gtggatagag
     1021 agcgtagttt ttcgtttcgc gcgacgcctt gtgaagagtg ccgcacaatg tattagtcag
     1081 aaggtagagc agaggtgttt ttcggggaag ggtctaagag aatccatcgt ggatgtttcc
     1141 acgatggatt ctccccgcat tgacctcccg accgtgccgt tcgattttca tagtgaggcg
     1201 tatcgacgag tttgcggcgt cgtcgctgcg tggaatttcc tattacagtc ctgccgctcc
     1261 ggaggaatag ctcgtgaagg atttcacacc tgcatacggt atcgtcccgc ccctactgac
     1321 tccttttaac gaggataagt cgattgactg ggatgcgtat gagcggctca tcgagtggca
     1381 tatcgagaag ggcgtatccg gtcttttcgt cgtgtgcgga tcgagcgaat actggcagct
     1441 gaccgacgac gaatccgtcg agatggcgaa gttcgccgtg aaagtcgccg gcgaccgcat
     1501 caacatcctc gccggctcaa cgaagtcgcc cgacgatgat ctcgatcgga acatcgccgt
     1561 tacgaagcgg atgtgggaag aaaccggcgt caaagggtgc tatctcacca cgccgcgcaa
     1621 gctcccgccc gaagatgaca tcatggttga atatcacatg aagatccacg atgccgtcga
     1681 gtgcccggta tacgcgtacg aaatgcccgg tggaacgaat tacaagttca gccctgaagc
     1741 gttcgcgaag attggccagg gcgaacgctt catcggcatc aaggacacca cgtgcgacct
     1801 cgagaaggtg cgcaaaaaga tcgaagcggc gaagggcacg atcctcatca tggaagcgaa
     1861 tacgccgaac ttcctcgaat cgctcaagct tggttcggtc ggtggcatca acacgtcggc
     1921 gaacgtcgag ccgggtctgt tcgtgatgct ctacaagctc ttccagaagg gcgatctcga
     1981 gactgc
//
LOCUS       DSCC01000240            2189 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034068, whole genome shotgun sequence.
ACCESSION   DSCC01000240 DSCC01000000
VERSION     DSCC01000240.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034068"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..808
                     /gene="rlmN"
                     /locus_tag="ENN56_02975"
     CDS             <1..808
                     /gene="rlmN"
                     /locus_tag="ENN56_02975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDS74481.1"
                     /translation="LLELSDGRRIETVAMHDGKRRTVCVSSQVGCALGCDFCATGQLG
                     LKRNLTTGEIIDQLCHAVDVFRASGHTEYPISNVVMMGMGEPLHNYESVVRAVRLMGL
                     EMGLAISVQRVTISTVGLVPQIRRLADEMPRVGLAISLNATTDEVRNRLMPINARWPI
                     SELMDAAKYMSDRAGRRRVTIEYALIAGINDTDDDARRLICLVSQFPCKINLIPFNPV
                     PDRPYRRPSVERIRRFHAMLVDRNLTATVRWSKGDDIAAACGQLQALNVG"
     gene            872..>2189
                     /locus_tag="ENN56_02980"
     CDS             872..>2189
                     /locus_tag="ENN56_02980"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS74482.1"
                     /translation="MRFVLRNAGEITGRFRRIAGRNSVSLTGRRSVVAALYAIAFVVA
                     SVATISSAQSLEDRMIEYRLDNGMLFLLVPMPNHAPVFSGVIMVKVGGVDEQIGQTGL
                     AHMFEHMAFKGTPWIGTTDYESEQVLLERIDSVAVIYTRLMASIPMEQRDALGSLERR
                     TLEAIRSHAPEELDPASAVATALAETLNVAGLQRYPYLDTYIRVKELSATLADLNEQH
                     RAFGIKDEFSQIVMVNGGVGFNAGTGKDYTVYYESFPANRLDLWMLLESQRFMYPVMR
                     EFYSERDVVIEERRMRSDDDPDGKLYENFMASAMIAHPYARPIIGWRSDIEQATAQEA
                     LDFRNRYYVPENSVGVLVGNFDVDEAKRLVTQYFGRIPAAEEPVPEIRTIEPPQAGER
                     RVFVEFDAEPQVMIGFHKPNYPNPEGYAFRVIANILRDVGNSSRFYQ"
BASE COUNT          472 a          573 c          661 g          483 t
ORIGIN      
        1 cctcttggag ctctcagacg gacgtcgcat tgaaaccgtc gcgatgcacg atgggaagcg
       61 gcggaccgtg tgcgtgtcct ctcaggtggg gtgcgcgctc ggctgcgatt tctgtgcgac
      121 cgggcagctc ggcctcaaac ggaatctgac aaccggcgag atcatcgatc agctctgtca
      181 tgcggtggac gtgtttcgcg cgtccggaca taccgaatat cccatctcaa acgtcgtcat
      241 gatgggtatg ggcgaaccgc ttcacaacta cgagagcgtc gtccgcgctg ttcggctcat
      301 gggcttggag atggggctcg caatatcagt ccaacgtgtt accatcagca cggtcgggct
      361 cgttcctcaa atccgacgcc ttgcggacga aatgccaagg gtcggtctcg caatatcgct
      421 caacgcgacg actgatgaag ttcgtaatcg gctgatgccg atcaatgcac ggtggccgat
      481 ttccgagctg atggatgcgg caaaatacat gtccgatcgc gcgggacgtc gacgggtgac
      541 aatcgagtac gcgctgatcg ccggcataaa cgatactgac gacgacgcgc ggcgactcat
      601 ttgtcttgtt tcgcagtttc catgtaaaat caatctcata ccgttcaatc cggtgccgga
      661 ccgtccgtac cgacgaccat ccgtggaacg gatcaggcga ttccatgcga tgctcgtcga
      721 tcggaacctg acggcgacgg tgcggtggag taagggcgac gacatcgcag cagcgtgtgg
      781 gcaactccaa gcgctgaatg tggggtagga attctgcaag gcgtcacgac gtttcgtatt
      841 taccaacgat cctaagcgaa acattttttc gatgcgattc gtcttacgaa atgctgggga
      901 aatcaccggt agattccgac gaatcgcagg gagaaacagt gtgtctctga caggaagacg
      961 aagtgtagtc gcagcgctgt atgcaatagc attcgtggtt gcgtcagtgg cgacgatcag
     1021 tagcgcgcaa tcgctcgaag atcggatgat tgagtatcga cttgataacg gtatgctgtt
     1081 cctgcttgta ccgatgccga atcatgcgcc ggtcttttcc ggtgttatca tggtcaaggt
     1141 cggcggcgtc gatgagcaga taggtcagac gggacttgcg cacatgtttg agcacatggc
     1201 gttcaaggga acgccgtgga tcggtacgac ggattacgaa tcggagcagg tgctccttga
     1261 gcggatcgac agcgttgcgg tcatctacac ccgactcatg gcgtcgattc ccatggaaca
     1321 gcgcgatgct cttggttctc tcgagcggag gacactcgag gcgattcgtt cgcacgctcc
     1381 ggaagagctc gatcccgctt cagcagttgc tacggcgctc gcggagacgt tgaacgtcgc
     1441 aggactccaa cgatacccgt atctcgatac gtacattcgc gtaaaggagc tttccgcgac
     1501 actcgcagac ctcaatgagc agcaccgggc gttcggaata aaagatgagt tctcgcagat
     1561 cgtcatggta aacggcggcg tcggatttaa cgccggcacg ggcaaggatt atacggtcta
     1621 ttacgagtcg ttccccgcga atcggctcga cctctggatg ctcctggaat cgcagcgttt
     1681 catgtatccg gttatgcgcg agttttacag cgagcgtgat gtcgtcatcg aagagcgtcg
     1741 catgcgttcg gacgacgatc cggatgggaa gctctacgag aactttatgg cgtctgcaat
     1801 gatcgcgcat ccatacgcgc gtccgattat cggttggcgg agcgacatcg agcaggctac
     1861 ggcacaggaa gcgctggatt tccggaaccg gtactacgtc ccggaaaact cggtcggtgt
     1921 tctcgtcggc aatttcgatg ttgacgaagc gaagcggctt gtcacgcagt atttcggacg
     1981 gattcctgca gccgaggagc cggtaccgga aattcggacc atcgaaccgc cgcaggcggg
     2041 tgagcgacgt gtcttcgtcg agtttgatgc cgagccacag gtgatgattg gatttcacaa
     2101 gccgaattat ccgaatccgg aagggtatgc tttccgagtt atcgccaata tcctgcgcga
     2161 tgtggggaat tcgtcgcgat tctatcagg
//
LOCUS       DSCC01000241            2189 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034063, whole genome shotgun sequence.
ACCESSION   DSCC01000241 DSCC01000000
VERSION     DSCC01000241.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034063"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            117..1190
                     /locus_tag="ENN56_02985"
     CDS             117..1190
                     /locus_tag="ENN56_02985"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDS74483.1"
                     /translation="MDYGVIMAGGKGTRLWPEGRIGLPKQLLKITGDRTLIEVAVDRL
                     SPLIPVERQLIVTTSDYAGMIAEALPHFPRENILAEPVGKNTAPCVGWAAVKIIEKDA
                     DAVMAVLTADHAITDEEAFRADLRAAITLAGEEPTVVTMGLVPTRPETGFGYIHHGTR
                     SRQADGRTAYAVDEFKEKPDLETAKHYVEAGTYLWNSGMFVMQARYALELFERHLPTH
                     FVQLSRIAKAIGTPAEGDVTASAYDAMHAVSIDYGIMENAESVYVIRGTFGWSDVGTW
                     PSLLQINEANSDGNLVLGEHVGIDTKNCIIRSNSRLVATLGVENLVIIETRDAVLVCT
                     KDQAQRVREVVQYLEANGRSDVL"
     gene            1326..>2189
                     /locus_tag="ENN56_02990"
     CDS             1326..>2189
                     /locus_tag="ENN56_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74484.1"
                     /translation="MQNVQYPQSIVGIVGKGATLVVRSTDSIRIEVLNAPDGMRPRTT
                     EVTGAGEVSLELTARNRCESGITRIRVTNTNTNESEEVAIPTKVWGPRMSFVCLVQSS
                     NVHIGWDPRPMAQYYRRDDDELVMYPGDSGGNHAWVHARKLERVFHKHNTPITWLIDD
                     AVAEEQATQIRDWHGRYGDAVAFLPRSFFYYNKRNYNLTSGTDELIDALRPQVRALES
                     AFAGTDWPLYCRTMGADQWVGSPGTRFVEAADALGFEGLWGIGYDHDTCDTSMYHKGT
                     PWDVYKPRQGNF"
BASE COUNT          514 a          592 c          645 g          438 t
ORIGIN      
        1 atactccttc gtattgcata ctcaactgaa tacggaaaac ggcgcgtgaa gcgaatcagg
       61 tcacacacgc gccgttttcc gtattcagtt gagtatgcaa tacgaaggag tatcgcgtgg
      121 attacggcgt aatcatggcc ggagggaagg gaacaaggct ttggccggaa ggtcggattg
      181 gactgccgaa acagcttctc aagatcaccg gtgaccgaac gctcatcgag gtcgccgtag
      241 atcggctttc tccactgatt ccggtcgagc ggcagctgat cgttaccaca agcgattacg
      301 ccggtatgat cgccgaggct ttgccgcact tcccgcgtga aaacatcctt gcggaaccgg
      361 tcggtaaaaa taccgcaccg tgcgtcggat gggccgcggt caaaatcatc gagaaagacg
      421 ctgacgctgt gatggcggtt ctgacagccg atcacgcgat taccgatgag gaggcgttcc
      481 gagcggatct ccgcgcagca attacgcttg cgggcgaaga accgacggtc gttacgatgg
      541 gtcttgttcc tacgcgaccg gaaacaggtt ttgggtatat ccatcacggc acacgttcac
      601 gacaagcaga tggtcgcact gcgtatgccg tggatgagtt caaggaaaaa cccgatctcg
      661 agacggcgaa acactacgta gaagccggta cgtacctctg gaactcaggc atgtttgtca
      721 tgcaggcacg atacgcgctc gagctgttcg aacggcatct cccaacgcac tttgtgcagc
      781 tctcccgtat tgcaaaggct attggtacgc cagcggaggg agatgtcaca gcctcggcgt
      841 atgatgcgat gcacgcagtg tccatcgact acggcatcat ggagaacgcg gagagcgtat
      901 acgtgattcg cggcacgttt ggctggtcgg atgtcgggac atggccatcg ctcctgcaaa
      961 tcaacgaagc gaactccgac ggcaacctcg ttttgggcga acacgtcggc atcgatacga
     1021 aaaattgcat catccgatca aatagtcgtc ttgtcgcgac actcggcgtc gaaaacctcg
     1081 taatcataga aacgcgcgat gccgtcctcg tctgcacgaa ggatcaggca caacgggtgc
     1141 gcgaagttgt gcagtacctc gaggcaaacg ggcgctcgga cgtgctctga actgactcgt
     1201 gaccgggtag ggaaacagtg ctgtgtttct ctacattctg tcagacgcgc cttccgctac
     1261 gaatgaatcc cacagaacct gatcgacggg aaattgcgac cgtctggctt tcaaggtggt
     1321 agtacatgca aaacgttcag tatccgcaga gcattgtcgg tatcgttggg aaaggtgcga
     1381 cgcttgtcgt ccgttcgacg gattcgatcc gcatcgaagt gctcaacgca cccgatggaa
     1441 tgcgtccccg caccaccgaa gtaaccggag cgggcgaggt gtcactcgaa ctgacggcac
     1501 gaaaccgatg cgaatcggga atcacgcgga tacgggtgac gaacacaaac acgaacgaat
     1561 cggaagaagt cgcgatcccg acgaaggttt ggggaccgcg tatgagcttc gtgtgccttg
     1621 ttcagtcgag taacgtacat atcggctggg acccccgccc gatggcacaa tactaccgtc
     1681 gcgacgatga cgaactcgtg atgtaccccg gtgactccgg cgggaaccat gcgtgggtac
     1741 acgcgcgcaa actcgaacgc gttttccaca agcacaatac gccgatcacc tggctcatcg
     1801 atgacgcggt cgctgaagag caagccacgc agatacgtga ttggcatggt aggtacggtg
     1861 atgcggtcgc gtttctcccg cggtcattct tctactacaa caagcggaac tacaacctca
     1921 cgagcggcac agacgagctc atcgacgcgc ttaggccgca agtccgggcg cttgaatccg
     1981 cgtttgccgg tacggattgg ccgctctact gccggacgat gggtgcagat cagtgggtcg
     2041 gttcaccggg aacgcggttt gtcgaagcgg ctgacgcact tggttttgaa gggctgtggg
     2101 gaatcggata cgatcacgac acgtgcgata cgagcatgta ccacaaaggc acgccttggg
     2161 acgtgtataa gccgcgtcag ggtaatttc
//
LOCUS       DSCC01000242            1874 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042082, whole genome shotgun sequence.
ACCESSION   DSCC01000242 DSCC01000000
VERSION     DSCC01000242.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042082"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..323)
                     /locus_tag="ENN56_02995"
     CDS             complement(<1..323)
                     /locus_tag="ENN56_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74485.1"
                     /translation="MQRIRDRLWMFSVEAGTDDQEYGIPLSRMTPIESCLTMGVSRLM
                     MITNGRDEPKPPLEPYFRALRPLDEVAWSLVGSGSRTGWAEGREVAILRDLAARYPNL
                     TGVYM"
     gene            complement(369..1232)
                     /locus_tag="ENN56_03000"
     CDS             complement(369..1232)
                     /locus_tag="ENN56_03000"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74486.1"
                     /translation="MKLGFLVQYNDAELGILTQIGMKSCQLIVPTHSPLNPAKASDDD
                     IKRAVDWFAEHDIEVSSIGDYRNNLDPDPKRRTESIEYISSLLDLAAKMGVDNVCTFA
                     GRDPAKSIADNIPMYKETWTPVAKKAEDLGLKVGFENCPNFQGWPFRGSNIAYLPEAW
                     DLMFDAIPSKALGIEYDPSHLICLLIDPVLVIKRYGDRIVHVHAKDAEVDWDYVRLNG
                     IMGRQSARHRAPGMGDANWAKIISALEECGYKGNVDIEGYHDPIYREDREVEGAIISF
                     KHLLNIIGEQS"
     gene            1571..>1874
                     /locus_tag="ENN56_03005"
     CDS             1571..>1874
                     /locus_tag="ENN56_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74487.1"
                     /translation="MSDRRLTVMIVPHNRKRIREITISQRTIFVAAVSLVMVALIGVG
                     YAVGFHIRSYQRAELQSLRAENQQLSTRIHEMAGSVVTLKTQMGEIERREEMLRVLA"
BASE COUNT          378 a          505 c          562 g          429 t
ORIGIN      
        1 tccatataca cgccggtgag attcgggtag cgagctgcga ggtcgcgaag gatggccact
       61 tcgcgacctt cggcccaacc ggtccggctg cctgagccaa ccaacgacca tgcaacctcg
      121 tcaagcggac gaagcgcgcg gaagtacggc tcgagcggag gtttcggctc atcacgtccg
      181 ttcgtaatca tcatgagccg cgagacgccc atcgtcaggc aggattcgat cggagtcatg
      241 cgactcagcg ggatgccgta ttcctggtcg tcggtgcccg cctcgacgct gaacatccag
      301 agtcgatcgc gtatgcgctg catggtgacc tccctcgacc ggcgccgcga gctgtgcggt
      361 tcgcctgatt acgactgctc gccgatgatg ttgagcagat gcttgaacga aataatcgcg
      421 ccttccactt cacgatcttc ccggtaaatc gggtcgtgat acccttcgat gtcgacgttc
      481 cccttgtacc cgcactcctc aagggcactg atgatcttcg cccagttcgc atcgcccata
      541 ccgggcgcgc ggtgacgggc cgactgccgt cccataatgc cgttcagccg tacgtagtcc
      601 cagtcgacct cggcgtcctt tgcatggacg tggacgatgc gatcgccgta gcgcttgatc
      661 accaagaccg gatcgatcaa gaggcagatc agatgcgagg gatcatactc gataccgaga
      721 gccttggacg ggatcgcgtc gaacatgaga tcccatgctt cgggcagata cgcgatgttc
      781 gagccacgga acggccaccc ttggaagttc gggcagttct caaacccgac cttgagaccg
      841 aggtcctccg cttttttcgc gacgggcgtc cacgtttcct tgtacattgg gatgttatca
      901 gcgatgctct tcgcgggatc gcgaccggcg aaggtacata cgttgtcaac gcccatcttc
      961 gcggcgagat cgagcagtga cgagatgtat tcgatggact cggtgcggcg ttttggatcg
     1021 ggatcgaggt tgttgcggta atcgccgatc gacgagactt ctatatcgtg ctcggcgaac
     1081 caatcgacgg ctcgcttgat gtcgtcgtcg cttgctttcg ccgggttgag cggggagtgc
     1141 gtcggaacga tgagttggca gctcttcata ccgatttgcg tgaggatgcc gagttccgca
     1201 tcgttgtact gcacgaggaa tccgagtttc acgaagctac tccttttggc gtgaacggtg
     1261 aattgacgcg atggttcgct tttgtcgcgg ttgaatgagt gtcggaaatg tcaccactac
     1321 catcgttcga tccaacggcg gggcgcggtc cgatgagtct gagtcgttcg tctttggcgt
     1381 gaaaggtctt gacaggatgt tttgcacgcg atattttcac cttggcttgt tgttacctga
     1441 gggcgcgcac tacgtcatat cattcagacc gcgttcgaac ggagcaggcc gccaagagta
     1501 tcgttcattt gaatacgtgt gtcgttattt tttgaaatag gtgtgtgtga ttaacaaaga
     1561 gcgagagaaa atgtcggatc ggcgcctgac agtgatgatc gttccgcaca atcggaagcg
     1621 gatacgcgaa atcacgattt cgcagcggac gatattcgtc gcggcggtgt cacttgtcat
     1681 ggtagcgctg atcggagtcg gatacgcggt tgggtttcac attcgttcgt atcagcgagc
     1741 ggagttgcag agccttcgcg cagagaatca acagctttcg acgcgaatcc atgaaatggc
     1801 gggcagcgtc gtcacgctaa aaactcagat gggcgaaatc gagcggcgcg aagagatgct
     1861 gcgcgtactc gcaa
//
LOCUS       DSCC01000243            1593 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052455, whole genome shotgun sequence.
ACCESSION   DSCC01000243 DSCC01000000
VERSION     DSCC01000243.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052455"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1593)
                     /locus_tag="ENN56_03010"
     CDS             complement(<1..>1593)
                     /locus_tag="ENN56_03010"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03710.13,HMM:PF08335.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74488.1"
                     /translation="GYDTVKPFLDDLQKAREAVIAIYDEVLGPPESPDDVSIVLDLPE
                     GSERAEELLAPYGFRDMARAHRDLRHLAYGHEVTATPCEPRPATIRLLPRLLEQLRTS
                     PDPDRGLTNVEQALRALGAVESFSDLLASHPGFLELLVTLCSGSQSLTDTVLRDPALI
                     DWMLYGGVLLRERKLIEIRLTLGAGLAGLADPEQIERTIQTFRKQEILRVGLRYLLGL
                     ADDEEIGHQLSAVADAVLGELYRRAVDDALERHGQPLNENGEPTGLVVLALGKLGSRE
                     MNFGSDLDLVFVHGAEGSTDTGRDNITVFSQIAQRIMRDVSEPTRHGTLYEVDARLRP
                     EGKNGPLTISLNGYRHYLSTRANTWERQALTRARLIAGPEPLRTTVRDAIDTYVYGPS
                     STDTIADVASMRSRMEDAAREKYGSRPNIKTGPGGLVDAEFIAQLGQILYGTDDESLR
                     GLDTVQSLRHLQERELLRSSVAETLISGYRTLRNLQIRLRIRDEHAHNVLPTDEDQLR
                     VLARTLGEPTADALERMIGDTMS"
BASE COUNT          307 a          458 c          500 g          328 t
ORIGIN      
        1 cgacatagta tcgccgatca ttcgttccaa cgcgtccgcg gtaggttcgc cgagcgttcg
       61 cgcgagtacg cggagttgat cttcgtccgt cggtagaacg ttgtgtgcgt gctcatcgcg
      121 gattcgcaag cggatttgta gattccgcag cgttcggtag ccggaaatga gcgtctctgc
      181 aaccgatgaa cggagcaatt ctcgttcctg aaggtgtcga agcgactgca ccgtatccaa
      241 cccacgaagc gactcatcat cggttccgta gagtatttga ccgagttgcg cgatgaattc
      301 ggcatccacg agaccacccg ggccggtttt gatattcggc cggctcccgt acttctcacg
      361 cgcggcgtcc tccatacgag accgcatcga agccacatca gcaatcgtat cggtagagga
      421 cggaccgtag acataggtat cgatggcatc gcgaacggtg gtgcgaagcg gttccggtcc
      481 cgcgatgagg cgtgcgcgtg tcaacgcctg ccgctcccac gtgttcgcgc gtgtgctcag
      541 gtagtggcga tatccgttca gagagattgt aagcggaccg ttcttccctt cggggcgaag
      601 gcgcgcgtcg acttcgtaga gcgtgccgtg ccgtgtcggc tcgctcacgt cgcgcatgat
      661 gcgctgcgcg atctgcgaaa acaccgtgat gttgtcgcga cctgtgtcgg tggagccttc
      721 agcgccgtgg acgaaaacga gatcgaggtc gctgccaaag ttcatctctc tcgacccgag
      781 tttcccgagc gcgagcacga cgaggccggt gggttctccg ttttcgttga gcggttgtcc
      841 gtggcgttcc aacgcgtcat ccacggctcg acggtagagt tcaccgagta ctgcatcggc
      901 gacggcactg agctggtgcc cgatctcctc atcgtcagcg agaccgagga ggtaccggag
      961 tccgacacgt aagatctcct gcttgcgaaa cgtctgaatc gttcgctcga tctgttccgg
     1021 atcggcaagc ccggcgagcc cggcaccgag tgtgagtcgt atttcgataa gcttccgttc
     1081 acggagaaga acgccgccgt agagcatcca atcaatgaga gcgggatcgc gcaggactgt
     1141 gtcggtcaga gattggcttc ccgaacacag cgtcacgaga agctcaagga atcccggatg
     1201 cgatgcgagg agatcgctga agctctcaac ggcgccgagt gctcggagag cctgttcgac
     1261 gttcgtcagt ccacgatcag ggtcggggct cgtgcggagt tgctcgagaa ggcggggaag
     1321 taaccgaatc gtcgccgggc gcggttcgca tggtgtcgcg gtaacctcgt gcccgtacgc
     1381 gagatgccgg agatcacgat gcgcgcgcgc catgtctcga aacccgtacg gagcgagtaa
     1441 ctcctccgcg cgctccgagc cttcgggcag atccaatacg atagagacgt catcaggcga
     1501 ttccggcgga ccgaggactt catcgtagat cgcgatgacg gcttcgcggg ctttctgaag
     1561 atcgtcaaga aacggtttta ccgtgtcgta tcc
//
LOCUS       DSCC01000244            2043 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037422, whole genome shotgun sequence.
ACCESSION   DSCC01000244 DSCC01000000
VERSION     DSCC01000244.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037422"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..846)
                     /locus_tag="ENN56_03015"
     CDS             complement(<1..846)
                     /locus_tag="ENN56_03015"
                     /inference="COORDINATES: protein motif:HMM:PF00326.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74489.1"
                     /translation="MRATRHTWMTDFHADNPYSLSLTRPITIVESEDVDVEYTAPGET
                     ESRTLACSVYEFIFPYRDQDRQPRIGRARMYLPPAIESRPARRSVPTLLSIHYEFGID
                     GSKRYLAEGWVVITPYGGGDDHMFNLVGSGMDHTLEMAKLVRRLGFVDLQRIGWFGGS
                     AGGYQCLITAESIWPVAAAVANVPLSDLEYDLECIEWSERYNRGITEERALTIPIVRF
                     VKGISDGTRRGSGNDPDVLWKNSAPIGASLIRSPLAIHTCSGDLLCPSPQIGPDFERV
                     PERGIM"
BASE COUNT          389 a          644 c          614 g          396 t
ORIGIN      
        1 catgataccg cgctcgggca cgcgctcgaa atccggtccg atctgcggcg acgggcagag
       61 cagatcaccc gagcacgtgt gaatggcgag tggactccgg atcagcgacg caccgattgg
      121 cgcggagttc ttccacagga catccggatc gttaccactg cctcgtctgg tgccgtcgga
      181 gattcccttc acgaaccgga caatcggtat cgtcagcgcc cgttcctcgg tgatgccgcg
      241 gttgtagcgt tcggaccact cgatacactc gaggtcgtac tcaaggtcgg aaagcggcac
      301 gttcgcaact gctgcggcaa ccggccagat cgattccgcc gtgatgagac actgatatcc
      361 gcccgccgag ccgccgaacc agccgatacg ctggagatcg acgaacccga ggcgccgcac
      421 gagctttgcc atctcgagcg tgtggtccat tcccgagccg acaaggttga acatgtggtc
      481 gtcgccaccg ccgtacggag taataaccac ccaaccttcg gcgagatatc gcttcgaacc
      541 gtcgatgccg aactcatagt ggatgctgag cagcgtggga acgcttcgac gcgcgggtcg
      601 agattcgatc gcgggcggga gatacatccg cgcgcggccg atgcgtggtt gccggtcctg
      661 atcgcggtag gggaagatga actcgtagac gctgcacgcg agtgtccgcg attcggtttc
      721 gcccggcgca gtgtattcaa catcgacatc ttcactctcg acgatggtga tcggtcgtgt
      781 gagcgagagc gagtatggat tgtccgcgtg gaaatccgtc atccacgtat ggcgagttgc
      841 ccgcatgtag tgtcctccga tacacgaagc tcgagtatgt atccgcagtg aaacgtgcga
      901 aaaggtatcc gttatccggc acgggaccaa ttgagcgacg tcattcgcgc gcgtatctca
      961 ccgcttcgta ccgtacacgc cgtatgtcgt ccggtaccgg gaatgtgtcg acagtaatcg
     1021 tccgctcatc catacgcgct atcggcaagc ctgtcgcgag cgtgtcggtt tcgattgcaa
     1081 cgtagtgttt cacatcgtcg ggtaccgggt gctgctcgcg cggtatcgct gtttccagcg
     1141 cgatacggtc atacccatcg cgtcgtgcga cgctttcgaa ctccggcata ccgttgtcga
     1201 agccgaatcc gtcgactgtc atgagtgtgc gtgcgcccga cgcgagatac cagcgcggac
     1261 caatcaacgg gatacacagt gcaccgaacg caccggcgaa tcgggcgcgg tcggtctcca
     1321 cttcgtcggc gcagtcgcta aagacgagat acgtgcccca ctcggatgtg atctgcacga
     1381 cgaccgcatt cggatccgtg tgcgagatcg tctcgacaag ctctacgcgt gtcacgacgt
     1441 cggcgggatc gtgcaccgca acgaagacgc tctcgacgtt atctccctcg cgaatcgcct
     1501 cgacacagcg aacgcgccgc cccgcctcct cccacgtctc ctgcccgggt ccgtgacccg
     1561 cctgaacgcg atcgacggga ctgagcatcc acaatcggta cgagcgacgc gcatcactcc
     1621 atacagcgcg ccacgatggc ggtggattgc cctcgcatcg cacgtcacgc agccccgcga
     1681 tgtgctccgt cgcttcgctt gcaccgtaat cgggaatcgg tggctcaggc ggcatgacga
     1741 taccttcgaa cgacagcgag ccgtctgcca tatcactcgc cgcgacttcg ctgaaggcgc
     1801 gccatgtatg tttgccgccg ccttttactc ggaagatatc cagcgtgaaa gtcgctccgt
     1861 cgggaccttt cgccatcgcg accgtgcggc ggtattcgcg gcactgcggg taacactcgg
     1921 acgacgcctc gacgacgctc atgtgcggcg cggtcaccat tcgatggaaa cgcggccgac
     1981 gtggggagtc gccatgctgc cgttgatgct cgtcatcaac gacgacgagg ttgtggctga
     2041 gcg
//
LOCUS       DSCC01000245            2282 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032144, whole genome shotgun sequence.
ACCESSION   DSCC01000245 DSCC01000000
VERSION     DSCC01000245.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032144"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="ENN56_03020"
     CDS             complement(<1..1733)
                     /locus_tag="ENN56_03020"
                     /inference="COORDINATES: protein motif:HMM:PF07940.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74490.1"
                     /translation="MDPIHPLLALIDTERPELETVHAAIERSDPSGALKDLVKYYRTR
                     EEPDAAAFSTPNANAVAAAERVIRREFTFYNEPGTVPPGDWDWTYKPGTDWEWTWALN
                     RHHFWRSLGAAYLATDNERYAQELATQVRTWVGNHPASTDDRSAWRTIEAGIRTGGPW
                     PSIIPIMKKSAAFTREVWLHYLRAIYEHAEFLIAHPKQNNWLLMESNGLLTCGLLFPE
                     FKRAADWVRIAIERLEAEMRRQVHPDGAHCEYSTGYIFVAISQFADACAKVDRVLDID
                     RGFSQEYRDLVVAMWEHVMYMMRPDGSQPMLNDGEMADVRPRLLAAGKRYDRSDFIYA
                     ATHGAEGTPPADASHRFPWVRRVVMRDAVSSHPWAEDALYGFFEAAPLGAGHVHEDML
                     TFELMAYGRPLIGTMGRMTYEHVPRRVYLVNGQGHNTVLVDGASQKWKRTDPDRSTWL
                     ASAPTDLPWRSTSEFDLAYARYEMPWSDDAVDGTVHERWMALRKRVDTARPYWLMRDR
                     LTRDGDTSEHVLRTLFHFYPGAELVLDGRTGTATVTYPSGVGVVLAFCDANAVTLDAA
                     IGQEDPVRGWY"
     gene            complement(2005..>2282)
                     /locus_tag="ENN56_03025"
     CDS             complement(2005..>2282)
                     /locus_tag="ENN56_03025"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74491.1"
                     /translation="VAPITQGGSRGVWEFRTEWSPDGSRLVFCRAIPGRPSELWVMNA
                     DGSDERALTLGWEYRGADHPRLVGIGFQTLERYGVTAANTSCRVFFG"
BASE COUNT          421 a          735 c          690 g          436 t
ORIGIN      
        1 ctgtaccagc cgcgcacagg atcttcctgt ccgatcgcag cgtcgagcgt caccgcgttt
       61 gcatcgcaaa acgccagcac cacgccgact cccgatggat acgtcaccgt cgcggtaccg
      121 gtacgtccgt cgaggacgag ttccgcgccg ggatagaagt ggaacagtgt tcggagcacg
      181 tgttccgacg tatcgccgtc gcgtgtcaaa cgatcgcgca tgagccagta tgggcgtgcg
      241 gtatccactc gttttcgcag cgccatccaa cgttcatgta ccgtaccgtc aaccgcatca
      301 tccgaccaag gcatctcata ccgcgcgtat gcaaggtcga actccgaggt gctccgccat
      361 gggagatcag tcggcgcgga tgccaaccac gttgaccggt cgggatcggt gcgtttccac
      421 ttctgcgatg cgccgtcgac gagtaccgtg ttatgtcctt ggccgttcac gaggtagacg
      481 cggcgcggaa cgtgttcata tgtcatgcgg cccatggtgc cgatgagcgg ccggccgtat
      541 gccatgagct cgaatgtcaa catgtcctcg tgtacgtggc cggcaccgag cggcgccgct
      601 tcgaagaagc cgtagagcgc gtcttcggcc cacgggtgcg acgatacggc gtcgcgcatc
      661 accacacggc gtacccacgg gaagcgatgg ctcgcatctg ccggtggagt gccctccgca
      721 ccgtgcgtcg cggcatagat aaagtcggag cgatcatacc gtttccccgc agcgagaagt
      781 cgagggcgca catcagccat ttcgccatcg ttcagcatcg gctgagagcc atccggccgc
      841 atcatgtaca tcacgtgctc ccacatggcg acgacgaggt cgcgatactc ctgtgagaag
      901 ccgcggtcga tgtcgagcac gcggtcgact ttggcgcacg cgtcggcgaa ttggctgatc
      961 gcgacgaaga tgtagccggt gctgtattcg cagtgcgcgc catcgggatg aacctgacgt
     1021 cgcatctccg cttcaagtcg ttcgatagcg atgcgcaccc agtccgctgc gcgtttgaac
     1081 tccggaaaca acagcccgca cgtgagcagg ccgttcgact ccatgagcag ccagttattc
     1141 tgtttcgggt gcgcgatgag gaactcggcg tgttcgtaga ttgcgcggag gtagtgcagc
     1201 cacacttcac gcgtaaacgc ggcgctcttt ttcatgatcg gaataatcga cggccacgga
     1261 ccgccggttc ggatacccgc ctcgatggtg cgccatgccg agcggtcgtc ggtgcttgcc
     1321 gggtggttcc cgacccacgt gcgcacctgt gtcgcgagct cctgcgcgta ccgttcgttg
     1381 tccgtcgcca agtacgccgc gccgagcgac cgccagaagt ggtgccggtt caacgcccac
     1441 gtccactccc agtccgttcc gggcttgtag gtccaatccc aatcaccggg tggcaccgta
     1501 cccggctcgt tgtagaaggt gaactcgcgc cgtatgaccc gttccgctgc tgcgacggca
     1561 ttcgcgtttg gcgtcgagaa cgccgcggca tccggctctt cgcgcgtgcg atagtacttc
     1621 acgagatctt tcagcgcgcc ggaggggtcg ctccgttcga tcgccgcgtg gacggtttca
     1681 agctccgggc gttcggtgtc gatcaaggcg agtaacggat gaatggggtc catggaatcg
     1741 ctttccgtgc ggagatggga gatacggcga tcattggaga aggtacgagg agagatggtg
     1801 aagcgcgaac ccttcgcttc gctacgggtt cgttaaggcg aagcgcgaat gctttcgcat
     1861 tcgttgcgct tcgccccacg tctccgagcg gaacgcgaga agacgcccgt tcggtttcgc
     1921 gcaggcggtt gaaaccgcgc actactcgtc tttaacccgt ctccgcaaga aagtccaccg
     1981 cgaacccttc gcttcgctac gggttcatcc aaaaaacacg cgacagcttg tattcgctgc
     2041 ggtaacgccg taccgttcca acgtctggaa accgatcccc accaaccgcg gatggtccgc
     2101 gccgcgatac tcccagccaa gtgtcaacgc gcgctcgtcg ctgccgtccg cattcatcac
     2161 ccagagctcc gacggacgcc ccggaatcgc gcgacagaac acgagacgcg acccgtccgg
     2221 tgaccactcc gtccggaact cccacacgcc gcgggaacca ccctgcgtga tcggcgcaac
     2281 gc
//
LOCUS       DSCC01000246            2317 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031460, whole genome shotgun sequence.
ACCESSION   DSCC01000246 DSCC01000000
VERSION     DSCC01000246.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031460"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            317..1525
                     /locus_tag="ENN56_03030"
     CDS             317..1525
                     /locus_tag="ENN56_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDS74492.1"
                     /translation="MFIHWGLYALPARHEWIKNREEIPDEKYEKYFRHFNPDLYDPTV
                     WAKAAKDAGMKYMVATSKHHEGFCLWDSAYTDYKATKTPYGKDVLQPMVKAFRDEGMK
                     VGFYHSLLDWHHPQFTIDGLHPMRNSKDYREREKNRDMSVYREYLYNQSEELLTKFEH
                     LDILWYDFSYPQAADGGKGRADWGSDKIYELVRRLRPDIILNDRLDLDEGWDVKTPEQ
                     WQPQEWVKVDGQPVVWEACQTLNGSWGYDRDNLDWKPVGMLLWMLIDSVAKGGNVLLN
                     VGPNGRGEFDERALATLKGIGAWMRLHGRSIYGCSQSEFDAPVDCRYTQNGNRLYLHI
                     MRWPLKHIHLKGLAGRVEYAQLLNDASEIFMRENVYGHNVPEDTLVLEIPVQQPNVEI
                     PVIELFLKDR"
     gene            complement(1600..1914)
                     /locus_tag="ENN56_03035"
     CDS             complement(1600..1914)
                     /locus_tag="ENN56_03035"
                     /inference="COORDINATES: protein motif:HMM:PF14561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS74493.1"
                     /translation="MAISNLEFFRAAVEKDPNNTFAQYSLAMEYRKTGALKDALEKFR
                     EIIRRDPAYVAAYLMAAQTAIDSDNESVAEEILRAGIETATSQNNSHAAGEMQDLLDS
                     IL"
     gene            complement(1939..>2317)
                     /locus_tag="ENN56_03040"
     CDS             complement(1939..>2317)
                     /locus_tag="ENN56_03040"
                     /EC_number="3.1.3.71"
                     /inference="COORDINATES: protein motif:HMM:PF04029.12"
                     /note="catalyzes the formation of (2R)-3-sulfolactate from
                     (2R)-2-phospho-3-sulfolactate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HDS74494.1"
                     /translation="EKIIIAGIVNVSAVAAWLLEQERDVTIVCSGTEGRLSLDDIHCG
                     GLLISNLFEPGFTPQLSDGVRLALQWFAANAGNAPYVLSSCTHGQRLIRQGFEDDVRW
                     CAQIDAVPVVPIHHGTGFTLLTT"
BASE COUNT          559 a          592 c          631 g          535 t
ORIGIN      
        1 cagacatacg tatggggagg gattacggtt tgctcaccgt gcttacactc agtggcgaaa
       61 cgacgctcaa gatgctgaag gatacggaag accgccccga tattgtggtc gagcatatcg
      121 gcaagctcac tccgctattt gaggcgcctg attcacgcat ttcgtaacgc ttccggttgc
      181 cccagacgcg aaccgtaata accttgatag cgagtttcgc ctttcgaatc atccatttcg
      241 caggagaaga gacgatgccc gttcagacga aaagacaccg ggtgatacgt catggttcac
      301 gcacgatcga tttggattgt tcatccattg gggactttac gcgctgccgg ctcgtcatga
      361 gtggatcaag aatcgcgaag agatccctga tgagaagtat gaaaagtatt tccgccactt
      421 caatccggat ttgtatgatc cgacggtttg ggcgaaagcg gcgaaagacg cgggaatgaa
      481 gtacatggtc gccacatcga agcaccatga aggcttctgc ctgtgggatt cggcgtacac
      541 cgattacaag gcgacgaaga caccgtacgg caaagatgtg ctgcagccga tggtaaaggc
      601 gttccgcgac gaaggtatga aggtcggatt ctaccattca ctcctcgatt ggcatcatcc
      661 acagtttacg atcgacggcc tccatccgat gcggaacagc aaagattacc gtgaacggga
      721 aaagaatcgc gacatgtcgg tttaccgcga gtacctctac aaccagtcgg aagaattgtt
      781 gacgaaattc gagcaccttg atattctctg gtacgatttc tcgtatccgc aagcggcaga
      841 cggcggcaaa gggcgcgctg actggggttc ggataagatt tacgagttgg ttcgacgact
      901 tcgccccgac atcatcctca atgaccgcct cgatctcgac gaaggctggg acgtcaagac
      961 acctgaacag tggcaaccgc aggaatgggt gaaagtagac ggtcaaccgg ttgtgtggga
     1021 ggcgtgccag acgctcaatg gaagttgggg ttacgaccgc gataatctcg attggaagcc
     1081 cgtcggaatg cttctctgga tgctgatcga ttcagtggcg aagggtggca atgtactcct
     1141 aaacgttggc cccaacggtc gtggcgaatt tgacgaacgt gcgctcgcga cactcaaagg
     1201 tatcggtgca tggatgcgac ttcacggccg tagcatctat ggatgctcgc agtcggagtt
     1261 tgacgcgccc gtcgattgtc gatacacgca gaacggcaat cgtctctatc tccatatcat
     1321 gcgttggccg ctgaagcaca ttcatctgaa aggcttggcc ggccgcgtgg aatatgcaca
     1381 gctactcaac gacgcgtctg agatcttcat gcgcgaaaat gtctacggtc acaatgtgcc
     1441 ggaagacacc ctcgtactcg agataccggt tcaacagcca aacgtcgaaa tccccgtgat
     1501 tgagcttttc ctcaaggatc gttgatttcg gtaatgaata aacgaaaaga gaggctcgtg
     1561 cgataaccgc gcgagcctct cttcgtttgt gcgcgttcgt tataagatcg aatcgagcaa
     1621 gtcttgcatt tcgccggcgg cgtgcgaatt gttttgcgac gtagcggttt cgatacccgc
     1681 tctcaggatt tcctcggcta cactctcgtt atcagaatca atagccgtct gtgctgccat
     1741 gaggtatgcg gccacatacg ccggatcacg tcggatgatc tcccggaatt tttccagcgc
     1801 gtctttcaag gcgcctgttt ttcgatactc catcgcgaga gaatactgtg cgaacgtgtt
     1861 atttgggtct ttttcaaccg cggctcgaaa aaactcaaga ttcgaaatcg ccaattgaga
     1921 gtcctccgga cctttgtatt acgtcgtgag caacgtaaaa ccggtaccgt gatgaatagg
     1981 aacgacgggt acggcgtcta tttgtgcgca ccaccgcaca tcgtcttcga atccttgccg
     2041 tatgagtcgc tgtccgtgtg tacatgagga taatacgtac ggagcgttgc cggcattcgc
     2101 agcgaaccac tgtagtgcaa gccgaacacc atcgctcagt tgcggagtga agcccggctc
     2161 aaacagattc gatatcagca gtccgccgca gtggatgtca tcgagagaaa gacgaccttc
     2221 ggttccggag cagacgatcg ttacatcacg ttcttgttca agaagccaag cggcgacggc
     2281 gctgacattt actatacctg cgatgatgat cttttcg
//
LOCUS       DSCC01000247            1552 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054330, whole genome shotgun sequence.
ACCESSION   DSCC01000247 DSCC01000000
VERSION     DSCC01000247.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054330"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..939)
                     /locus_tag="ENN56_03045"
     CDS             complement(<1..939)
                     /locus_tag="ENN56_03045"
                     /inference="COORDINATES: protein motif:HMM:PF01966.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS74495.1"
                     /translation="MEERLEPNWTHRDEADAAELTVLVVDDDEPQRLLLEHLLSRKGY
                     SVASVADGQTALEYTAAIVPDVILLDVILPDMDGFEVCRRIRATESLREVYILMLTGL
                     SDREARLQGFDAGADDFVSKPFDSLELTTRMRAVAKINRFRRLIEVRDRNTVLAEELQ
                     KAYNATLEGWSAALELRDHDTQGHTRRVANMTIALASHIGVDEEDKVHIYRGALLHDI
                     GKIGIPDSILHKAGALTDEERELMRQHPIFAYDMLKHITYLRKALEIPYCHHEWWSGE
                     GYPRGISGEEIPLSARVFAVVDVWDALSFNRPYRNAL"
     gene            1192..>1552
                     /locus_tag="ENN56_03050"
     CDS             1192..>1552
                     /locus_tag="ENN56_03050"
                     /inference="COORDINATES: protein motif:HMM:PF00795.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDS74496.1"
                     /translation="MARMARIVSISFPGIRGDMTPVERSRAAVDDACRRIDEAAFGDP
                     DLIVLPETFTGLGAGGDTFFTSAEPIPGPTTDALAEKARKYATYIVFGILRRCDGKLY
                     NASVLLGRDGSVRGIYHK"
BASE COUNT          342 a          411 c          427 g          372 t
ORIGIN      
        1 caatgcgtta cggtacgggc ggttgaagct cagcgcatcc catacgtcga caacggcgaa
       61 gacgcgtgcg gaaagcggaa tctcttcacc tgagataccg cgcggatacc cttccccgct
      121 ccaccactca tgatggcaat acggtatttc cagcgcctta cgcagatagg taatgtgttt
      181 gagcatatca tacgcgaata tcggatgctg ccgcatgagt tcgcgttctt cgtccgtcaa
      241 cgctccggct ttatgaagga tcgagtcggg aataccgatt ttcccgatgt cgtggagcaa
      301 agcgcctcgg tagatatgaa ccttgtcttc ttcatctacg cctatgtgcg aggcgagagc
      361 gatcgtcata ttcgccactc gtcgagtatg tccctgcgtg tcatgatcgc gaagctccag
      421 tgctgcactc caaccttcca gcgttgcgtt atacgctttc tgaagctcct ccgccagtac
      481 ggtgttccgg tcgcgaacct cgatcaggcg acgaaatcgg ttgatttttg caacggcacg
      541 catccgcgtc gtcagttcga gcgagtcgaa cggtttcgat acgaagtcgt cggcaccggc
      601 atcgaagcct tggagccgag cttcccgatc gcttagaccg gtgagcatga gaatgtacac
      661 ctcgcgaaga ctctcagtcg ctcgaattcg acggcacact tcgaagccgt ccatgtctgg
      721 gagaatgacg tcgaggagta tcacgtcggg aacgatcgca gctgtatact caagcgccgt
      781 ctgaccgtcg gcgactgatg caactgagta tcccttacgc gagagaaggt gttcgagaag
      841 aaggcgctga ggttcgtcgt cgtcgacgac aagtacagtc agctcggcgg catcggcttc
      901 atcccgatga gtccaattcg gttcgagtct ttcttccatt gatttcgcac atatcggttt
      961 caaggtgaaa aatcactatg agactgtggt taccctacat acagagtagt caaaacgctt
     1021 ctggtcgata aaatcgagtt tgcacgactc aacgtatgcc tctgtgcaat ggattgtacc
     1081 ccttcggtat gtgtgtgaca agctgacgtc cgaccaatga gcgttggaat cgacgaaaac
     1141 caagcgtact tctcttgtga ccagatcacc ttttagacgg aggagcaagc gatggcgcgg
     1201 atggctcgaa tcgttagtat cagttttcca ggaatccgtg gcgatatgac gccggttgaa
     1261 cgatcgcgcg ccgcggtcga tgatgcctgt cgtcgtatag acgaggcggc cttcggagat
     1321 ccggatctta ttgtgcttcc ggaaacgttt accggtctcg gtgccggtgg tgatacgttt
     1381 ttcacgagcg ccgaaccgat tccgggtccc acgacggacg cgcttgcgga aaaagcgcgt
     1441 aagtacgcta catatatcgt gtttgggatt ctgcgacggt gtgacggcaa gctttacaat
     1501 gcctcggtac ttctcggacg cgacggttcc gtacgtggaa tctatcataa aa
//
LOCUS       DSCC01000248            1845 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043045, whole genome shotgun sequence.
ACCESSION   DSCC01000248 DSCC01000000
VERSION     DSCC01000248.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043045"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(36..545)
                     /locus_tag="ENN56_03055"
     CDS             complement(36..545)
                     /locus_tag="ENN56_03055"
                     /inference="COORDINATES: protein motif:HMM:PF05198.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74497.1"
                     /translation="MRPRVDERIVAARVFLVDTDGSAVGERERDEAITIASTAGLSLV
                     EVQPDADPPVCKLMAPDRVARFNERVSEPKERPLEVRVLAFRTTMREADIVLRVRHAQ
                     AYLAVGGAVELRVERRSARDTDAKTLLERLLQMLGERCSLPVETRNTKSEIVALVCPR
                     NESPGTVAE"
     gene            complement(562..>1845)
                     /gene="thrS"
                     /locus_tag="ENN56_03060"
     CDS             complement(562..>1845)
                     /gene="thrS"
                     /locus_tag="ENN56_03060"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDS74498.1"
                     /translation="LQRIYGTSFPQQKQLDEFLHMIEEAKRRDHRILGSRLDLFSIQQ
                     VVGSGLIHWHPKGAIVRDLIERFWKDEHQKHGYSLVYTPHIASDNLFRISGHLEKYDA
                     MYSPMEIDGENYRLKPMNCPFHIQIYQSHTRSYRDLPIRYAELGTVYRYERSGVLHGM
                     LRVRGFTQDDAHIFCRPDQLEDEVLSTLELMKYLMQTFGLGYDVFLSTRPEQSFGTDD
                     EWEFATKALANTLERAGVPYTVDAGEGVFYGPKIDVKLVDVLGRPWQGPTIQVDLQLP
                     QRFGVEYVGSDNQTHNAIMVHRALLGSLERFIGCLIEHYAGAFPLWLAPVHAMVLPLT
                     DDQSDAATALVKRMREQGFRADVDLSSEKIGAKIRDAELQRIPYMLVLGKREIENGTV
                     SVRARDRGDLGAKAIDDVFAVLADEIKTKSLESMM"
BASE COUNT          392 a          561 c          488 g          404 t
ORIGIN      
        1 ccctttcatg tgtggttggt ccgcaccggt tttcgttatt cagccaccgt acccggactc
       61 tcatttcgcg ggcacaccag tgcaacgatt tcgcttttcg tgttgcgcgt ctcgacgggc
      121 aacgaacagc gctcgccgag catctgtagc agtcgctcta acagtgtttt cgcgtcggta
      181 tcacgtgccg aacgtcgttc gacacgaagc tccaccgcgc cgcccaccgc gagatacgct
      241 tgcgcatgac gaacgcggag aacaatatcg gcctctcgca tcgtcgttcg aaatgccagt
      301 acgcgaacct cgagcggtcg ttccttcggc tcgctcactc gctcgttgaa gcgtgcgacg
      361 cgatccggcg ccataagttt gcaaaccgga ggatcggcgt ccggctgaac ctcgacgaga
      421 ctcagccccg ccgtactcgc gatcgtgatc gcctcgtcgc gttctcgctc gccgaccgca
      481 gatccgtcgg tatctacaag aaacacgcgc gcggcaacga tgcgctcatc tacgcgggga
      541 cgcatacgca ctcccgcggt atcacatcat tgattcgagc gatttcgtct tgatctcgtc
      601 cgccaaaacg gcgaacacat cgtcaatcgc cttcgcaccg aggtcgccgc gatctcgcgc
      661 acgcaccgat acggtcccgt tttcaatctc gcgcttaccg agtacgagca tatacggaat
      721 ccgctgaagc tccgcgtcgc gaatcttcgc accgatcttc tcgctgctca gatcgacatc
      781 ggcgcggaat ccctgctcac gcattcgctt caccaaagcc gttgcagcgt cgctctgatc
      841 gtcagtgagc ggaagaacca tcgcgtgaac gggtgcgagc cataacggga aagcgcccgc
      901 ataatgctcg atcaaacacc caatgaaccg ttcgagcgaa ccgagtaacg cacgatgcac
      961 catgatggcg ttgtgcgttt gattatccga cccaacatac tccacgccga agcgctgggg
     1021 aagctgcaaa tctacctgaa ttgtcggtcc ctgccaaggt ctgccgagta cgtcgacaag
     1081 cttaacgtcg atctttgggc cgtaaaatac cccttcgccc gcgtcaaccg tgtagggcac
     1141 tccggcgcgc tcaagcgtat tcgcgagtgc cttggtcgca aactcccact cgtcatccgt
     1201 accgaagctc tgctcgggac gcgtgctcag aaacacgtcg tagccaagtc caaacgtctg
     1261 catgagatac ttcatcagtt cgagcgtgct gagtacttcg tcctcaagct gatcggggcg
     1321 gcagaatata tgcgcgtcgt cctgagtaaa cccgcgaaca cgtagcattc cgtgcaaaac
     1381 accggaacgc tcataccgat acaccgttcc aagctcagcg taacgaattg gcagatcacg
     1441 atagctacgg gtgtgcgact ggtagatctg gatgtggaac gggcagttca tgggcttgag
     1501 ccgatagttc tcaccatcga tttccatcgg tgagtacatc gcgtcatatt tctcaagatg
     1561 cccactaatg cggaaaagat tgtcactcgc aatgtgcgga gtgtaaacga gtgagtatcc
     1621 gtgtttctga tgctcgtctt tccagaatcg ttcgatgagg tcgcgaacta tcgcaccctt
     1681 tggatgccag tggatgagcc ccgatccgac aacctgctga atgctgaaga gatcaagccg
     1741 cgaaccgagg atgcggtgat cgcgtcgctt cgcctcttcg atcatgtgta ggaactcatc
     1801 gagctgtttt tgctgcggga agcttgttcc gtagatgcgt tggag
//
LOCUS       DSCC01000249            3030 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022074, whole genome shotgun sequence.
ACCESSION   DSCC01000249 DSCC01000000
VERSION     DSCC01000249.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022074"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            18..1334
                     /locus_tag="ENN56_03065"
     CDS             18..1334
                     /locus_tag="ENN56_03065"
                     /inference="COORDINATES: protein motif:HMM:PF00733.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HDS74499.1"
                     /translation="MYQYVRHMYTTGRETFFRGIERLLPGTMMFVSRNGIRTKRYRDV
                     PLESEPVMSGSVQQYANTLREIIDDAVRLRLRSDVPVGCYLSGGIDSSSVVALAQPRL
                     DGMNTFSLAFDEGPEFDERRFVAMVRDKYPTEHYEISPTMRESWEILPSVVWWLDEPV
                     VASPTISQYFLSKIARERVTVCLGGQGGDELFGGYYRFFPRYLKMMLRDTIRGRRPLA
                     DVAPTLRNFIRHINIVGIGRVRTKMRKHASMMELVGAPISDAVHDTELETMSDVSIAD
                     PMNRIFYWEIKNYLPGLLHTEDRMSMAVSLESRVPLLDYRVVEFAASIPPELKMHHLV
                     TKYILRESMRGITPDPILDRFDKRGTPGPLVKWFAGGLNDDVRSILSDKKTRERGLFD
                     SVGVEKAISDHRKNGAYGEQIWMMVCIELWHRTFIDVDNASGPITI"
     gene            1344..2933
                     /locus_tag="ENN56_03070"
     CDS             1344..2933
                     /locus_tag="ENN56_03070"
                     /inference="COORDINATES: protein motif:HMM:PF14052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74500.1"
                     /translation="MGTRSLLIALVFTTAIAGASPYVPLDDRAYRDIESLRARGHAVV
                     PAATLRPYTAKHIAVGAYHLTLHPELSDYEREMAGRLLARFERHLPDSLRTHPNTTKS
                     YLVADVNPRLIAQMDQIRNDIPRTLRLPATWRNQSDQIRSINTLTVDASVSDNVSVQV
                     RLEADTDGVQDNDYNGRLKTYRLGMTGYLPSTYVSWENEWAVVAFGRQPLIWGPGRHG
                     STHLSENAPAIDMLSLRVDAWKWFHFSWFTGELSGVVDGMYFEGGRRYITGHRMAITP
                     THWLEIGAGEIAHTSKAGWGFSFWQSNPVGVLMVEEVNRGPGDSNILLSCDITVRPIH
                     GVETYAEWTVDDYSLDKKVPHFLAWTVGGQWEAPLGWDRFGIGAEYSRVMRWTYNYYT
                     DSPYQKHVNVRAVMGHFIGSDSDLLTVDATWEARSGYRFTGITTLRRLGETRTATPFP
                     VRTSGIPFGYHTEPFPSGTVERTITSGMLAESPPFYGAYVTVGAFIQSFDNFGNVVGA
                     SKTAYHVRFGLDWHIPVRFPG"
BASE COUNT          683 a          854 c          825 g          668 t
ORIGIN      
        1 aaccgaatcc gcgcgcaatg taccagtacg ttcgccacat gtataccacc ggtcgagaga
       61 cattcttccg cgggatagaa cgacttcttc caggtacgat gatgttcgta tctcgaaatg
      121 ggattcgcac caagcggtac agggatgtac cgctcgaatc cgaaccggtg atgtcgggat
      181 cggtgcagca gtacgcaaac acgcttcgcg agattatcga cgacgcagtg cggttacgac
      241 tacgtagtga tgtcccagtc gggtgttatc tgagcggcgg tatcgattcg tcaagcgtgg
      301 tggcgctcgc acaaccacga ctcgacggga tgaacacgtt ttcgctcgca ttcgacgaag
      361 gaccagaatt tgacgaacga cgtttcgtcg cgatggttcg cgacaaatac cccacggaac
      421 actacgaaat aagtccaacc atgcgtgaaa gttgggagat tctcccatcg gttgtctggt
      481 ggctcgacga accggtcgtc gcgtctccaa caatatctca atatttcctc tcgaagatcg
      541 cccgtgaacg cgtgaccgta tgcctcggcg ggcaaggcgg tgacgaactt tttggcgggt
      601 attaccggtt ctttccgcgg tacctcaaga tgatgttgcg cgacaccata cgaggacgac
      661 gtccgctcgc cgatgtcgcg cctaccttgc gtaacttcat ccgacatata aacatcgttg
      721 ggattggccg cgtccgaacg aaaatgcgta agcacgcgag catgatggaa ctcgtcggcg
      781 caccgatcag cgacgcggta cacgacacag aactcgagac aatgtctgat gtgtcaatcg
      841 ccgatcccat gaatcggata ttctattggg aaatcaagaa ttacctaccg gggcttcttc
      901 acacggaaga ccgcatgagc atggcggtat cgctcgaatc acgtgtgccc ctcctcgatt
      961 accgcgtcgt cgagttcgcc gcatcgattc cgcctgaact caaaatgcac catcttgtga
     1021 cgaagtacat cctacgtgaa tcgatgcgcg ggataacgcc ggatcccatc ctcgatcgct
     1081 tcgacaaacg cggaacgccc ggtccgctcg tgaagtggtt cgccggtggt ctgaacgacg
     1141 acgtgcgttc cattctttcg gataagaaga cccgcgagcg cggcctgttc gattcggtcg
     1201 gcgtcgagaa agcaatctca gatcatcgaa aaaacggggc atacggagag caaatctgga
     1261 tgatggtgtg tatcgagtta tggcaccgca cattcattga tgtcgataac gcgtcgggac
     1321 caatcacaat atagggcggc cggatgggca cacgatcgtt actcatcgca ctcgtcttta
     1381 cgacggcgat cgccggtgcg agcccgtatg tcccgcttga cgaccgcgct taccgcgaca
     1441 tcgaatctct tcgtgcacgg ggacacgccg tagtacctgc cgcgacgctt cgaccgtata
     1501 ccgcgaaaca cattgccgtc ggagcgtacc accttacatt gcatcctgaa ctcagtgatt
     1561 acgaacgaga aatggccgga cggctgctcg cgagattcga gcgacacttg cccgattcac
     1621 tccgaacgca tccaaatacc acgaaatcgt atctcgtcgc cgacgtaaat ccgcgtctca
     1681 tcgcgcaaat ggaccagatc cgaaacgaca tccctcgcac tcttcggctg ccggcaacgt
     1741 ggcgcaacca aagtgatcag atacgttcga tcaatacgct gaccgtagat gcctccgtca
     1801 gcgataacgt ttccgttcaa gtccgcctgg aggccgatac agatggcgtt caagacaacg
     1861 actacaatgg acgattgaag acgtaccgac tcggaatgac gggataccta ccgtctacct
     1921 acgtgagttg ggaaaacgaa tgggcggttg ttgcattcgg gcgacagccc ctcatctggg
     1981 gtccggggcg tcacggaagc actcatcttt ctgagaacgc tccggcgatc gacatgctta
     2041 gtttgcgcgt tgatgcgtgg aaatggtttc atttcagttg gttcaccggc gaattgagtg
     2101 gcgtcgtaga cggtatgtat ttcgaaggcg gacgacggta tatcaccggt caccgtatgg
     2161 cgatcacacc gacacattgg ctggaaatcg gagccggcga aatcgcgcac acgagtaagg
     2221 cgggatgggg gttttcgttc tggcagtcga atccggtcgg tgtactcatg gtcgaggagg
     2281 tcaatcgcgg ccccggtgat tcgaacatcc tgttgtcgtg cgatattacc gtccgcccga
     2341 ttcacggagt ggaaacgtat gccgaatgga cggtcgacga ctactcgctc gataaaaaag
     2401 tcccgcattt tcttgcatgg actgtcggcg gacagtggga agcaccgtta ggttgggatc
     2461 gattcggtat cggcgcggag tactcacggg ttatgcgctg gacgtataac tactacaccg
     2521 attcgcctta ccagaagcac gtgaacgtcc gcgccgtaat ggggcacttt atcggatcgg
     2581 acagcgatct actcactgtc gacgcaacgt gggaagctcg tagcggttac cgattcacgg
     2641 gaatcacgac acttcggcgt ctcggagaaa cgcgtacggc gactccattc ccggttcgca
     2701 cgtcgggaat cccgttcggc taccataccg aaccatttcc atcgggtacc gtcgagcgca
     2761 ccatcacgtc cggaatgctc gcggaatcac cgccgtttta cggcgcgtat gtcaccgtcg
     2821 gtgcatttat ccagtcgttc gataacttcg gaaacgttgt cggagcctcg aaaaccgcct
     2881 atcacgttcg attcggtctc gattggcaca tccccgtacg attccccgga taacctattt
     2941 tccctctttt ttcgcctcga gtcctttttc gatggcgttt acgatcgttt ccagacccat
     3001 gatgcgcgca acattcttat cctgcccggg
//
LOCUS       DSCC01000250            1816 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043958, whole genome shotgun sequence.
ACCESSION   DSCC01000250 DSCC01000000
VERSION     DSCC01000250.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043958"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            232..>1816
                     /locus_tag="ENN56_03075"
     CDS             232..>1816
                     /locus_tag="ENN56_03075"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74501.1"
                     /translation="MTSIQSGGIVHSLFASQPRRLALAFTMTLITLTGTDASSQEARP
                     EYSSGIVLVRIDGANKPSQNSALQQFGLADPQPVISVSPRMRPTIGGTQTWYAYAIDD
                     DRDPGSLADSLATIGVVAQPNYLYRLLDTPRTSVTPNDPRFAEQWAAKSIGLEQVWQQ
                     FQEISRDSVIVAIIDTGIDYNHEDLTPRMWRNLAEINGIDGIDDDGNGYIDDYIGWDF
                     TDAPGLPGVGDFLERDNDPMDANGHGTAVAGIIGAEIDNGIGIAGAAPTARLMALRAG
                     AELEFGGGFLEEDDIAAAVIYAVENGARVINMSFGDVVSTPLMYDVVQFAENQGVVVV
                     ASAGNERTSKLLYPAAYDETIAVGSIDEANEKSHFASWGENIDLVAPGSAVLTTRLNN
                     GYGLVWGTSFSGPYVAAACATVISYAPYLSPAQVRTLIRAATIDIGSPGWDPEFGAGR
                     LHLPTLLAATSSPTAEIVSPRRPGGADVSFDWSIAIGGAPPVAWDLSYGVGDEPRLWT
                     KIAVGASDSPIDTVTGIASV"
BASE COUNT          393 a          524 c          511 g          388 t
ORIGIN      
        1 ccgctatgtc actacaattg agcgagcaga cacctgctac acgtatcgaa gatagagccg
       61 ccgcgcaacg catgctgttc ggttggatcg taaaagcgac actttgaagc aataaacgtc
      121 tccggtccga cgttaccgtg cacgctgcgt tggaacacca tcgttctcgc cgacgtatcc
      181 tttgagtagt cgtacggtat tgcccgccga cgacgattcc cgttcgactg catgacctcg
      241 attcagagcg gaggaatcgt gcacagcctc ttcgcgagcc aacctcggcg tcttgcgctc
      301 gcgtttacga tgacactcat tacgcttacg ggcaccgacg cctcgtctca ggaagcacgt
      361 ccggagtatt catccgggat cgtgctcgta agaattgacg gcgctaacaa gccatcacaa
      421 aactccgcgc ttcaacaatt cggtcttgct gatccgcaac cagttatctc cgtatcgcct
      481 cggatgcgtc caacgattgg tggtacgcag acatggtacg cgtacgcgat cgatgacgat
      541 cgcgatcccg gctcgctcgc agactcgctc gcgacaatcg gagttgtcgc ccaacctaac
      601 tacctatacc gactcctcga tacaccccgt acgtccgtca ctccaaatga tcctcgtttc
      661 gcggagcagt gggccgccaa gagcatcggt ctggaacagg tatggcagca attccaggag
      721 atttcccgag atagcgttat cgtcgcgatc atcgatacgg gtatcgacta caaccacgaa
      781 gatctcacac cacgtatgtg gcgtaatctc gctgagatta atggtattga cggaatagac
      841 gacgacggga atggatacat cgacgactac atcggatggg atttcacgga cgcaccggga
      901 cttccgggtg tcggcgactt cctcgaacgt gataacgacc cgatggacgc caacggacac
      961 ggcaccgcag tcgcagggat cattggcgcg gagatcgata acggaatcgg gatagccggt
     1021 gcggcaccga ctgcgcgact catggcgctt cgtgccggtg ctgaactcga gttcggcggt
     1081 ggatttctcg aagaggacga tattgcggcg gccgttatct acgccgtaga aaacggcgct
     1141 cgagtgatca atatgagctt tggcgacgta gtctcaactc cgctcatgta tgacgtcgtg
     1201 cagtttgccg agaatcaagg tgttgtcgtc gttgcatccg ctggaaacga gcgaacgtcg
     1261 aagctcctct atccagccgc atacgacgaa acgattgcgg tcggatcaat cgatgaggcg
     1321 aacgaaaagt cgcatttcgc cagttggggc gaaaatatcg atctcgtcgc tcccggatcg
     1381 gcggtgctca cgacgagatt gaataacgga tatggcctcg tgtggggcac gtcgttttcc
     1441 ggaccgtacg tcgctgcagc gtgcgcgact gtcatcagct acgcacctta tctttcaccg
     1501 gcgcaagtac gaacactcat tcgcgcggca accatcgata ttggttcacc gggatgggac
     1561 cctgaattcg gtgccgggcg actccatctt ccgacgcttc tcgcggcgac gtcctcgcca
     1621 acagcagaaa tcgtctcgcc gcgacggccg ggtggtgccg atgtttcatt cgactggtcg
     1681 attgcaatcg gaggcgcgcc tccggtcgcg tgggatttga gctacggagt cggtgacgag
     1741 ccacgacttt ggacgaagat cgcagtcggc gcttcggata gtccgattga caccgttacc
     1801 ggcatcgctt ctgtcg
//
LOCUS       DSCC01000251            2313 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031545, whole genome shotgun sequence.
ACCESSION   DSCC01000251 DSCC01000000
VERSION     DSCC01000251.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031545"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..447
                     /locus_tag="ENN56_03080"
     CDS             <1..447
                     /locus_tag="ENN56_03080"
                     /inference="COORDINATES: protein motif:HMM:PF00717.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HDS74502.1"
                     /translation="NGEKVRVVCVPILTRIPAGSPVAVEDATPVGIGLEGWLRVPDPR
                     DENAFALIAAGDSMEPIIHDGEPFLVSPRRRIGFTNGLAVIRLPGDEVCVKHVRFSSR
                     GVEVGSANPRYPVRRFSAESIRLVGEALLVRDIGSVDTSIPCVMQN"
     gene            532..1392
                     /locus_tag="ENN56_03085"
     CDS             532..1392
                     /locus_tag="ENN56_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDS74503.1"
                     /translation="MDSRPHPSPLVSYHGGHTLFDGTGEPEAFVVRAIEQGFTAFGFS
                     EHMPPPDPYSYTDFPTFDEARHLFDGYVEEIFRLKSAYRDELPVLVGVEIEYLPDKES
                     VITDFLNAYPFDYTVGSVHFIGNHTFDFSKEAYDAGVRAFGGMDPWVEEYYRLVRSLL
                     DMGVTDVLGHLDIIKIFADDVAGYRSERVRGAERETLEAALRAGVVLDVNARGLIKAC
                     AEVYPGPELLAEAQRMGVPATLGDDSHRVDQVGARLDQALDAIRAAGYLTLTALLPEG
                     ESIIRRALPI"
     gene            1412..2122
                     /locus_tag="ENN56_03090"
     CDS             1412..2122
                     /locus_tag="ENN56_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74504.1"
                     /translation="MVSERSAIARIASRIVRATVFLPMLLRHRKARSAVYLFAVALAF
                     CALHDSSAVLVPFLIWMVPGTSVAMLPGEPAALAAAQMLRSGTMSIPETALTVAFIAG
                     MAAGISAIADHALLTWVRERDRVQTLMDHPIAQRVLSLFDTMPFATIYLFSFLPLPFF
                     AVRVVSIAARYPIERYSLATALGRFCRYFVIALLGAVFILPDWAIIGAFVLLVVAFLV
                     IRNRHRNGNDKSENVTIP"
BASE COUNT          422 a          658 c          711 g          522 t
ORIGIN      
        1 aatggtgaga aggttcgcgt cgtctgcgtg ccgatcctga cgcgaattcc agcggggagt
       61 cctgtcgccg tagaggacgc gacgccggtc gggatcggac tggagggatg gctccgcgtg
      121 cccgatccgc gggacgaaaa cgccttcgca ctcatcgccg cgggagattc catggaaccg
      181 attattcacg acggcgaacc gtttctcgtg agtccgcgcc gacgtatcgg atttacgaac
      241 gggcttgcgg tgattcgact gcccggcgac gaggtctgcg tgaaacatgt gcgtttcagc
      301 tcgcgcggtg tagaggtggg cagtgcgaat ccacgatatc ccgtgcgacg cttctccgcg
      361 gaatcgatac ggttggtcgg cgaggcgctg ctcgtgcgcg atatcggcag tgtggacaca
      421 tcgataccgt gcgttatgca gaactgatac ggtcggcttg cgagacaccg cggcgtgggg
      481 gtaacttcct gtgcatattg actcagttaa ccggtttgca cggagtacca ggtggatagt
      541 cgacctcacc cgtcaccgct tgtctcatac catggcggtc atacgctgtt cgatggcaca
      601 ggcgagccgg aagcgttcgt cgtccgggcg atcgagcagg gattcacggc gttcggcttc
      661 tcggagcata tgccgccgcc cgacccgtac agctacaccg atttcccgac gttcgacgaa
      721 gcgcgtcatc ttttcgatgg atacgtggag gagattttcc gtctcaagtc cgcgtatcgt
      781 gacgaattgc cggtactcgt cggcgttgag atcgaatatc tacccgacaa ggaatccgta
      841 atcaccgact tcctcaacgc gtatccattt gactacacgg tcggatccgt ccatttcatt
      901 gggaatcaca cattcgattt ttcgaaagag gcatacgacg cgggcgtacg cgcgtttggt
      961 ggcatggacc cgtgggtcga agagtattac cgcctcgtgc ggtcgctgct cgatatgggc
     1021 gtgactgacg tgctcggtca tctcgatatc attaaaatct tcgccgatga cgtagccggt
     1081 taccggagcg aacgcgtgcg gggagcggaa cgcgagacat tggaggcggc actgcgtgca
     1141 ggtgtcgtac ttgacgttaa tgcgcgcggt ctcatcaaag cgtgtgcgga ggtatatccg
     1201 gggccagaac tcctcgcgga agcgcagcga atgggagttc cggcgaccct cggtgacgac
     1261 tcgcaccgcg tcgatcaggt cggcgcgcgg ctcgatcagg cgctcgatgc cattcgagct
     1321 gcgggatatt tgacgttgac ggcgttgtta cccgaaggag agagcatcat ccggagggct
     1381 ttaccgatct gaggttggcg tgagcgacga gatggtatcc gaacgttcgg caattgcgcg
     1441 gattgcgtca cgaatcgtcc gcgcgacggt gtttcttccg atgctccttc gacatcggaa
     1501 ggcgcgatct gccgtgtacc tgttcgctgt agccttggca ttttgcgcgc tccacgattc
     1561 gagcgccgta ctcgttccgt ttctgatctg gatggtgccg ggtacctccg tcgcaatgct
     1621 ccccggagaa cccgctgcgc tcgcagcggc acagatgctg cgcagcggca ccatgtcgat
     1681 tcccgagacg gcgctgacag tcgctttcat tgccggtatg gcggccggta tctcggcgat
     1741 cgccgatcac gcgcttctca cgtgggtgcg tgagcgcgac cgtgtccaaa cgctgatgga
     1801 ccatcctatc gcacaacgtg tgctcagcct cttcgatacg atgccgtttg cgacgattta
     1861 tctcttctcg ttcctcccgc tgccgttttt cgcggttcgg gtcgtcagca tcgcagcgcg
     1921 gtatccgata gagcgctaca gcttggcgac ggcactcggt cgattttgtc ggtatttcgt
     1981 tattgcgttg ctcggtgccg tgttcatatt gcccgactgg gcgattatcg gcgcgtttgt
     2041 cctccttgtc gtagcgtttc ttgtgatccg gaaccgacat cggaacggga acgacaaatc
     2101 cgagaacgtc acgattccgt aacggcgaaa tcggtctcct gcagacaact ccatgacgga
     2161 tcggtcgtga ttgaccgccc cgtataccac accgatattc gaattatcca tgggaacgag
     2221 cgtccggcga aacctatgcc ggaggaggtc cggagaaatg ttcgctcgat cagtacgccc
     2281 gcctgcggtc gccgatcaat tctacccggg atc
//
LOCUS       DSCC01000252            2908 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023333, whole genome shotgun sequence.
ACCESSION   DSCC01000252 DSCC01000000
VERSION     DSCC01000252.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023333"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            2..694
                     /locus_tag="ENN56_03095"
     CDS             2..694
                     /locus_tag="ENN56_03095"
                     /inference="COORDINATES: protein motif:HMM:PF01120.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74505.1"
                     /translation="MQPNILLFNMGDPDFRWIGNEQGYAPLGTRNVVDRIPFSIHETA
                     GNVIKPTWLPAECDARMRERNWFYSENDEHTVKSVEELVGMWYYSVGRGCNLLLNIGP
                     DRRGLLPESDAESLLGLGAEVRRRFAHPLATLSACERNANSWTWTPEKAELVDHVIIG
                     ENILRGEHARRFAVRADVGEMITVFEGTAIGYNQACSFPPIRARRFVVEVTESEGETE
                     LARLEFHRTKTF"
     gene            728..>2908
                     /locus_tag="ENN56_03100"
     CDS             728..>2908
                     /locus_tag="ENN56_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74506.1"
                     /translation="MHNSLFIRDLLSRTNNDPLRIDRRNAHRGVAGDPSATPQTGEFA
                     LVGAWHVSGDSDAGEAWKLAVADLDEFLGLMNVLRDCAASRTITLGNDATLPPRAYRL
                     TKNPKRIEVLASDAAGVWAGVAALEREMRSRRGPILAEGTTERRARWRWQVSQGPWGG
                     NYSVPDFSPEYLSDDAFKLYAHYGVNSMMIYGDLLCYVQSDVLPELNHPDYDYHIGML
                     ADAAKRAANYGVQFTYVVVGPKLRSTHPVFQAHPSTRGSGVTSPTGDYSIHCLCSSDE
                     TVAAFYDETFTKLFQSVPELAGMYFIIATESFYHCRMWRDPTHRCPRCYDQMQEDVVG
                     SMLGRFADAVQRVKPDAFVFGWPYNTDPWDRPDGYEFVRRLPTNVGLFNQIDRKHWYE
                     KDGYRKLIWDYSIDYIGPATQVVDRIKIAKERGLTLFTKTETGIGLEVFQFPYVPSMN
                     RLADKWQVMRDLEPDGVHQSWLFFGMFGTRAEELAYWAAYEPCSRDEYLRMMAERDFG
                     PDAADFVISAWTEMSEAVGHIPSITLEGYYVGPSFLGPCHPLIPDDSTPVPDEFDAVL
                     FFLQEGEETFSRRRNEVRSSLVMSTLPESARAISIEWDGDSDGWDIVLREYRSAADHA
                     RTSWQHLRDAVPHARTTTDKKNLLEETLLTELVFRTFVSCANTVEFLMERRKMETTGD
                     ADALNRMREIARDELENAVAALPIYDQCPWLDIAERTDGYFTPCR"
BASE COUNT          649 a          826 c          826 g          607 t
ORIGIN      
        1 catgcaaccg aacattctgc tgttcaacat gggcgatccg gatttccggt ggatcgggaa
       61 tgaacagggt tacgcaccat tgggcacgcg aaacgttgtc gaccgcattc cgttttccat
      121 tcacgaaacg gcgggtaacg tgatcaaacc aacgtggcta cctgccgaat gcgacgcgcg
      181 gatgcgcgag cggaactggt tctacagcga gaacgacgag cacacggtca aatctgtcga
      241 ggaattggtc ggaatgtggt actacagtgt cggacgcgga tgcaatctgt tgttgaatat
      301 tggaccggac cgacgcggat tgcttcccga aagcgacgcg gagagccttc tcgggctcgg
      361 agcagaggta cgcagacgct tcgcccatcc acttgcgacg ctgagcgcat gcgaacgaaa
      421 cgcgaatagt tggacgtgga cacccgagaa agccgaactc gtcgaccatg tgatcatcgg
      481 cgagaacatc ttacgcggcg aacacgcacg tcgttttgcc gtgcgagccg atgtcggtga
      541 gatgattact gtattcgaag gaacggcaat cggatacaat caagcctgtt ctttcccacc
      601 gattcgcgca cgcaggtttg tggtcgaggt caccgaatca gagggagaga ccgaactcgc
      661 gcgcctcgaa tttcaccgca ccaaaacgtt ttaatcgtat cgtcacgccg ccttttctgg
      721 gggatacatg cacaattcgc tcttcatccg cgacctcctc tcacgaacga acaacgatcc
      781 gctgcgtatc gacaggcgaa acgcacatcg cggtgtcgcg ggcgatccgt cagcgacacc
      841 tcaaacgggg gagtttgctc tcgtcggcgc atggcacgta agcggcgatt cagacgctgg
      901 tgaagcatgg aaacttgccg tcgcagacct cgatgaattc ctcggactca tgaacgtttt
      961 acgcgattgt gccgcaagtc gaacgattac tctcggaaac gacgcgacat taccaccgcg
     1021 cgcgtatcgt ctcacgaaga acccgaagag aatcgaggtg ctcgcatctg acgcggcggg
     1081 agtatgggca ggagttgccg cactcgagag agaaatgcgt tcccgacgtg gcccaattct
     1141 cgctgaaggc accacggagc gccgtgcccg ttggcgttgg caagtgagcc aaggtccgtg
     1201 gggcgggaat tacagcgtgc cggacttctc gccggagtat ctcagcgacg acgccttcaa
     1261 actgtacgcg cattacggcg tgaatagcat gatgatctac ggcgaccttt tgtgctacgt
     1321 ccagagtgac gttcttcctg agttgaatca tcccgactac gattaccata tcggcatgct
     1381 tgccgacgcc gcaaaacgag cagccaacta tggcgtccaa ttcacgtatg tcgtcgttgg
     1441 accgaagttg cgctcgacgc atccggtttt tcaagcacat ccttcgactc gcggaagtgg
     1501 cgttacctca cctaccggtg attattcaat tcattgtctg tgctcgtccg acgaaacggt
     1561 cgcagcattc tacgacgaaa cgtttacgaa gctctttcaa tcggtgcccg aactcgccgg
     1621 aatgtatttc atcatcgcaa ctgagtcgtt ctaccactgc cgaatgtggc gtgatccgac
     1681 gcatcgatgc ccgcgctgtt acgaccaaat gcaagaggac gtcgtaggtt cgatgctcgg
     1741 gcgattcgca gatgcggtgc agcgtgtgaa acctgacgcg ttcgtatttg gctggccgta
     1801 caacactgac ccgtgggatc gaccggacgg atacgagttc gtgcgacgct taccgacaaa
     1861 cgtcggtctc ttcaaccaga tcgaccggaa gcactggtac gaaaaggatg ggtaccggaa
     1921 actcatctgg gactacagca tcgactacat cggcccggcc acacaggtag tcgaccgtat
     1981 caagatcgcg aaagaacgcg gtttgacgct tttcacaaaa accgaaaccg gtatcggact
     2041 cgaagtattt cagtttccgt acgtcccttc gatgaatcgg ctcgcggaca agtggcaggt
     2101 gatgcgagac ctcgagccgg acggcgtgca tcagtcgtgg cttttcttcg gtatgtttgg
     2161 aacgcgcgcg gaggaactcg cgtattgggc cgcgtacgag ccgtgttctc gtgatgagta
     2221 cctccgcatg atggccgaac gcgatttcgg tccggacgcc gccgatttcg taatctccgc
     2281 gtggaccgag atgagcgaag cggtgggaca cattccgtcg attacccttg aaggttacta
     2341 cgttggaccg agctttctcg gtccgtgcca tccgcttatt cctgatgact cgacacccgt
     2401 ccctgatgag ttcgacgcgg tgctgttctt ccttcaggag ggcgaggaga cgttctcgcg
     2461 tcgccgtaac gaagtccgtt cgagcctcgt catgagcacg ctgcctgaat ccgcacgcgc
     2521 gatttctatc gaatgggacg gtgacagcga cggctgggat atcgttctgc gagaataccg
     2581 ttcggccgcg gatcacgcgc gcaccagttg gcaacatctc cgcgacgccg ttccacacgc
     2641 acgaacgacg acggacaaga aaaaccttct cgaggaaaca ctcctcaccg agttggtgtt
     2701 ccggacgttt gtgtcctgcg cgaataccgt cgaatttctc atggagcgac gaaaaatgga
     2761 gacaacggga gatgcagacg cgttgaaccg tatgcgtgaa atcgcccgtg atgaactcga
     2821 aaacgccgtc gccgcgctcc cgatctacga ccaatgcccg tggctcgaca tcgccgaacg
     2881 cactgacggg tatttcaccc cctgccgc
//
LOCUS       DSCC01000253            2385 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030314, whole genome shotgun sequence.
ACCESSION   DSCC01000253 DSCC01000000
VERSION     DSCC01000253.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030314"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..678)
                     /locus_tag="ENN56_03105"
     CDS             complement(<1..678)
                     /locus_tag="ENN56_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HDS74507.1"
                     /translation="MLERLAALEERFEEITRLMADPDVFSDLSRYTELKKEHHHLEPI
                     VTASREYRSILNDLEEARHILNDGSDPDLVELAEAEIEPLEARSEALEQRLREFLLPK
                     DPNDDRNTIVEFRGGTGGDEATLFAGDLYRMYARYAENRGWSMEVISSSPSSAGGFKE
                     MIVLVKGAGAYGRLKYESGVHRVQRVPTTETQGRIHTSTASVAVLPEADDDVDIEINP
                     NDLHIDVY"
     gene            complement(757..1749)
                     /locus_tag="ENN56_03110"
     CDS             complement(757..1749)
                     /locus_tag="ENN56_03110"
                     /inference="COORDINATES: protein motif:HMM:PF07136.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDS74508.1"
                     /translation="MTVLASEESIERTVDRPIGGQAVIEGVMIRSPERVATAVRSPDG
                     NISVQAYPYISLVKRVRGLGLPIFRGIVTFAEMTVLGVRTLTYSASIAIPDEQTESGW
                     KHALLDWLITATAIVAGLGLFLALPLAVANAFGLTNNPLAFNFVAGTVRATILLGYLW
                     VLGRVPDLRRVFAYHGAEHKAVFAYEADEELTVATARRYSRFHPRCGTSFLLIVVFVA
                     IVAYAVIDATFAVLMERAPTLIERIATHFLFLPIVAGASFEALKASGTYRNSAIVRAL
                     IAPGLWLQRLTTREPDDAQLEVAVAAARASLGLDPAFTPRADSRTVDEESFVAV"
     gene            complement(1767..1973)
                     /locus_tag="ENN56_03115"
     CDS             complement(1767..1973)
                     /locus_tag="ENN56_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDS74509.1"
                     /translation="MKTDIHPEYKTTQISCACGNVVDIRSTSKAVLKVEICSACHPYF
                     TGKQKLIDTAGRVDAFNRRYGRKS"
     gene            complement(2059..2265)
                     /locus_tag="ENN56_03120"
     CDS             complement(2059..2265)
                     /locus_tag="ENN56_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74510.1"
                     /translation="MILIRERRWALGPALAVIVATLVAVAIFWGDSRFRFPIVPYYTV
                     FASVALGALWARLRGHTLQPASKS"
BASE COUNT          512 a          641 c          708 g          524 t
ORIGIN      
        1 gtatacatcg atatgaaggt cgttcgggtt gatctcgatg tcgacatcat cgtcggcttc
       61 cggtagcacc gcgaccgacg ccgtactcgt gtgaatccgt ccctgcgttt ccgtggtggg
      121 tacgcgttgg acacggtgga cgccgctttc gtacttcaga cggccgtacg ctccggcgcc
      181 tttcacgagc acgatcattt ccttgaaacc gcccgcgctc gaggggctgc tgctgatgac
      241 ttccatgctc cagccgcgat tctcggcgta tcgcgcgtac atgcgatata ggtctcccgc
      301 gaagagcgtt gcttcgtcgc caccggttcc tccgcgaaac tctacgatgg tattccggtc
      361 gtcgttcggg tctttcggga ggagaaactc gcgaaggcgc tgttcgagtg cttcacttcg
      421 tgcttcgagt ggctcgatct cagcttcggc gagttccaca agatccggat cgctaccgtc
      481 gtttagaata tgtcgtgctt cttccagatc gttgagtatg ctgcggtatt cgcgtgaagc
      541 ggttacgatc ggttcgaggt gatggtgctc ctttttgagc tcggtgtaac gcgaaaggtc
      601 cgagaaaaca tcgggatccg ccatcagccg cgttatctct tcgaagcgtt cttccagtgc
      661 ggcaagacgt tccaacatcg attcaatagt cctctcacgg cgtaagccgt atgataaata
      721 cagggagtga gacgttgtat tggcgtgttg aggtagctac accgcgacga acgactcttc
      781 gtcaacggta cgcgaatcgg cgcgcggggt aaatgcgggg tcgagaccaa gcgaggcgcg
      841 tgcggctgct acggcgactt caagttgcgc atcgtctggt tcacgcgtgg tgaggcgctg
      901 tagccagagt cccggtgcga tgagtgcgcg cacgattgcg ctgttccgat acgttcccga
      961 cgctttgagc gcttcgaacg atgcgcctgc gacaatcggc aggaacagga agtgcgtggc
     1021 aatacgttca atgagcgtgg gcgcgcgttc catcaatacg gcgaacgtcg catcgatgac
     1081 cgcgtacgct acgatcgcga cgaacacgac aatcaagagg aagctcgtac cgcagcgcgg
     1141 atggaaacgg ctatagcggc gcgctgtggc gacggtgagt tcctcatcgg cttcgtacgc
     1201 aaagaccgcc ttgtgctcgg cgccgtggta ggcgaaaaca cgtcgcagat caggcactcg
     1261 tccgagaacc catagatagc caaggagaat cgtcgcgcgc acggttcccg caacgaagtt
     1321 gaatgcaagc gggttattgg taaggccaaa cgcgttcgca acggcaagcg gtagcgcgag
     1381 gaacaatccg agtcccgcta cgattgccgt tgcggttatc agccagtcga gcagcgcgtg
     1441 tttccaaccg ctttccgtct gctcgtccgg tatggcaata cttgcggaat aggtcagtgt
     1501 acggactccg agcacggtca tttccgcgaa ggtaacgatg ccgcggaata ttggcagacc
     1561 gagtccgcgt actcgcttta cgaggctgat gtacgggtac gcctgtacgg agatgttgcc
     1621 atcggggctt cgtacggcgg tcgcgacgcg ttccggcgag cggatcatga cgccttcgat
     1681 taccgcctgc ccaccgatcg gcctatcgac ggtccgttcg atcgattctt cggaagcgag
     1741 cacggtcatg gagagacctt cgatgcttag ctcttccgtc cgtaacgccg attgaaggcg
     1801 tcgacgcgtc cggcggtgtc gatgagcttc tgcttgccgg tgaagtacgg gtggcacgcg
     1861 gagcagattt cgaccttgag gacggctttg gatgtcgagc ggatatcgac aacatttccg
     1921 cacgcgcagg atatctgcgt cgtcttgtac tcgggatgaa tatcggtctt catgttcgat
     1981 ttactccctg tatgtgcgtg aacagtttta ttgaaagaca aaaacgaaat atacgcatga
     2041 ataacgtatc cgacaagctt aagatttcga agcaggttgc agtgtgtgtc cgcgtagccg
     2101 cgcccataaa gcaccaagtg cgacgcttgc gaatacggtg taatatggca caatcgggaa
     2161 tcggaagcgg ctatcacccc agaatatcgc caccgcaacg agcgtcgcaa cgatgaccgc
     2221 gagcgctggg cccagtgccc atcgtcgctc acgaatcagt atcacgattc cgacaacaga
     2281 aatcggaacg aagatgacat caagcgaata gaggatgatt ttcagatgga tcggtatacg
     2341 agtaagtaaa ctaaagaacc gcgcgaactt catcgtgatg aaaaa
//
LOCUS       DSCC01000254            2111 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1035769, whole genome shotgun sequence.
ACCESSION   DSCC01000254 DSCC01000000
VERSION     DSCC01000254.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1035769"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="ENN56_03125"
     CDS             complement(<1..1038)
                     /locus_tag="ENN56_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74511.1"
                     /translation="MRGVVYCLIGAVAVLSPLSAVADSRGPSVLDGYVGSARHALAVE
                     AGRNSTDVVETPDDLGQSTVRPSSRKSPAKAFILSLLVPGLGEFYAGNMLRGAVFFGI
                     EAASWTAWNVYTKKGDDARADFIAFQTEHWNFERYDTYRRAVWQDLAARSNVFDPSRP
                     LAQRQQDSLTVLIGTHHYDDCCGQATPKTDDQYEMIGKYYRFSYGWDDVTVWDNPDKE
                     LRDEVVFSAGSGETWGTNLDAWLSYRGGELVRAEINDSTGVRQVRYLEQVHSANRETY
                     TRMRKDANDYHSTAKTFTTVILFNHIVSAIHAARITSKLNETGVYEPPRTSMRMELYD
                     TGSDYVPMMVLR"
     gene            complement(1077..2087)
                     /locus_tag="ENN56_03130"
     CDS             complement(1077..2087)
                     /locus_tag="ENN56_03130"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDS74512.1"
                     /translation="MGFMLFAFVFAVARDAVLLLTITGSKLGALVHGVFGGENVTSSP
                     DMGRRSFFVQASNVGIVAFAGGLTGLGLWSARKTPTVRNVEIHVPGLASAFDGFRIAQ
                     ITDLHIGATVKRSFVESVARTVNELKADLIAVTGDLVDGSVRSLRDDLMPLGSLTAPF
                     GTYFVTGNHEYYSGADQWVDYLRDELKWNVLLNEHRVLERGEERLVIAGVTDFSRRNA
                     DNPHRSDPATAIHGSPDGVPRVLLAHQPMSVNAAAQAGIDVQLSGHTHGGQFFPWNLV
                     VHMVQPYVSGLNRHKNTQIYVSKGTGYWGPPLRVGAPSEVTLITLRADSSSGEKSRRS
                     EA"
BASE COUNT          463 a          641 c          546 g          461 t
ORIGIN      
        1 ccgcagaacc atcatcggta catagtcgga cccggtatcg tagagttcca tacgcattga
       61 tgtgcgtggg ggttcgtata cgcctgtctc gttcagctta ctcgtgatcc tcgccgcgtg
      121 aatcgcgctg acaatatggt tgaagagaat taccgtcgtg aacgtcttcg ccgtgctatg
      181 gtaatcgttc gcatccttgc gcatccgcgt gtaggtctca cgattcgccg aatgcacctg
      241 ttcgaggtac cgcacctgcc tgacgcctgt tgagtcgttg atttcggccc gcaccaactc
      301 accaccccga tagctcaacc acgcatcgag attcgtcccc catgtttcac ccgagcccgc
      361 gctgaataca acctcgtcgc ggagttcttt gtccggatta tcccagacgg taacgtcgtc
      421 ccaaccgtaa ctgaatcgat agtacttgcc aatcatctcg tattgatcgt cggttttcgg
      481 cgtcgcttga ccgcagcagt cgtcgtaatg atgcgtgccg atgagaacgg taaggctgtc
      541 ctgttggcgt tgtgcgagcg gtcgggacgg atcgaacacg tttgatcgcg ccgcaaggtc
      601 ttgccaaacc gcacggcggt acgtgtcata tcgctcgaaa ttccagtgtt ctgtttgaaa
      661 cgcaataaag tcggcgcgcg catcgtcgcc ttttttggta tagacgttcc acgcggtcca
      721 cgacgcagct tctatcccga aaaatacggc gcctcgtagc atatttcccg cgtaaaactc
      781 acccagaccc ggaacaagaa gcgaaagaat aaacgccttt gcgggtgact tccgactgct
      841 cggacgaacg gtagattgac caagatcgtc cggcgtctcg actacgtcgg tgctgtttcg
      901 cccagcttcc acggcaagcg cgtgcctcgc gcttccaacg tacccatcga gcaccgaagg
      961 cccacgcgaa tctgctaccg ctgagagggg gctcaatacc gcaacggccc caattaagca
     1021 atacaccacg ccgcgcatct tctcgctcct tactgaggtt ctcgtcgtct gtttcgtcag
     1081 gcttcggatc gcctcgattt ctcaccgctg gacgaatcgg cacgcaacgt aatgagcgtg
     1141 acttccgacg gtgcgcctac gcgtaacggc ggaccccaat atcccgttcc cttgctgaca
     1201 tagatctgcg tattcttgtg ccgattcaaa cccgaaacat acggctgcac catgtgcacg
     1261 acgaggttcc acgggaagaa ctggccaccg tgcgtgtgtc ccgaaagttg tacgtcgata
     1321 ccggcttgcg cagcggcatt gacgctcatc ggctggtgcg cgagcaacac ccgcggtaca
     1381 ccgtccggtg aaccgtgaat cgctgtggcc ggatccgatc gatgagggtt atcagcgttc
     1441 cgacgactaa agtccgtgac acccgcgatt acaaggcgct cttcgccgcg ttccaacacc
     1501 cgatgctcgt tcaggagaac gttccacttg agttcgtcac ggagatagtc tacccactga
     1561 tcggcgcccg agtagtattc gtgatttccg gtaacgaaat acgttccaaa cggtgctgta
     1621 agcgacccga gcggcattag atcgtctcga aggcttcgta ctgatccatc gacgagatca
     1681 ccggtaaccg ctataagatc ggctttgagt tcgtttaccg tacgagcgac actttcgacg
     1741 aatgaccgtt ttaccgttgc accaatgtgg agatcggtga tctgcgcgat gcgaaaccca
     1801 tcgaacgcag acgccagtcc cggtacatga atctcgacat tgcgcacagt aggcgtttta
     1861 cgcgccgacc acagaccaag tcctgtcaga ccacctgcga acgccacgat acccacgttc
     1921 gaagcctgca caaaaaaact acgtcgcccc atgtccggtg aggatgtaac gttttcgcca
     1981 ccgaacacgc cgtgcaccaa cgcaccgagt ttcgatccag taatcgttag gaggagaacc
     2041 gcgtctcgtg cgacggcaaa cacgaacgca aagagcatga atcccatgct taggtagccg
     2101 acccacgcga g
//
LOCUS       DSCC01000255            1832 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043447, whole genome shotgun sequence.
ACCESSION   DSCC01000255 DSCC01000000
VERSION     DSCC01000255.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043447"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1037
                     /locus_tag="ENN56_03135"
     CDS             <1..1037
                     /locus_tag="ENN56_03135"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01408.20,HMM:PF02894.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74513.1"
                     /translation="LKFGIIGCRHGHVHSQITELLAIPGAICAGVYDDDDASVQSVLD
                     RHPVSRIDSPDRLLDDPDVTLIGTAEVNRRKPDVILRALNAGKNAIADKPLCTSIEDL
                     NAIEKTATEKGLRVGLQLTERFGPANRRMKSLVDSGAVGHVANVMCWRPHRLGRPGRP
                     DWMFDDDQYGGILVDLAIHDADIIRWLCGGVFTEITAYEQNFGNPSDTDFHDIGTLLG
                     RLSTGPTGFVRTDWFTPTPSPVHGDTRFLVTGTEGMIEVRTAGDLWAKKPDTSGQILV
                     MTNTDGVKVEKPEPPSKTLVQDFVDSIREGTTPDIMNRDVFEATRATLMARMSSKLSR
                     TVLRGETLAT"
     gene            1088..>1832
                     /locus_tag="ENN56_03140"
     CDS             1088..>1832
                     /locus_tag="ENN56_03140"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74514.1"
                     /translation="MKLACQEGMAPGTTLEEKLANIEQAGYEGVELWGGGLVDRLEEV
                     KRATKKASVPVTTICAGYRGTPLSNDLATRKQAADDIVMLLDAAGELGATGLIFVPIF
                     GKAQINDLSPWKSAEELERELLVELLGDWAEHARKAGTLLLLEPLNRYETHLIKTLRD
                     AVQICEQVNNPKGLKMMADFFHMSIEERDIPTSIGAAGDWIAHMHLADSTRELPGYGH
                     TDFAAGFAALREIGFDGYMALECGIPGDDK"
BASE COUNT          419 a          541 c          543 g          329 t
ORIGIN      
        1 cactgaaatt cggcattatc ggatgccgac acggtcacgt tcacagccaa atcacggagt
       61 tgctcgctat tcccggcgcg atatgcgcgg gcgtttacga tgacgatgat gcctcggtgc
      121 aaagcgtact cgaccggcat ccggtgagcc ggatcgattc gccggaccga ctcctcgacg
      181 accccgacgt cacactcatc gggacggcgg aagtcaaccg caggaagccc gatgtcatcc
      241 ttcgcgcgct gaatgccggg aagaatgcaa tcgccgacaa gccgctctgt acgagcatcg
      301 aggacctcaa cgcgattgag aaaacggcga cagaaaaagg tttgcgcgtc ggtctccagc
      361 taaccgaacg gtttggtccg gcgaatcgtc gcatgaaatc actcgtcgac agtggcgcgg
      421 tcggccatgt tgcgaatgtc atgtgctggc gaccgcaccg actgggtcga ccggggcgtc
      481 ctgactggat gttcgacgac gatcagtacg gcgggatcct cgtcgatctc gcgatacacg
      541 acgcggacat catccgttgg ctctgcggcg gtgtatttac cgagattacc gcatacgagc
      601 agaacttcgg caacccgtcg gacacagact tccacgatat cggcacgctc ctcggtcggc
      661 tgagtacggg tccgaccgga ttcgttcgca cagactggtt cacaccgacg ccgtcaccgg
      721 ttcacggtga cacgcgtttc ctcgtcacgg gcaccgaggg tatgatcgaa gtgcgaacgg
      781 cgggcgatct gtgggcgaag aaacccgata catccgggca aattctcgtc atgacaaata
      841 ccgacggcgt caaggttgaa aagccggagc caccgtcaaa gacacttgta caggatttcg
      901 tcgacagtat ccgtgaaggc acgacaccag atatcatgaa ccgcgacgta ttcgaagcca
      961 ctcgcgcgac attgatggct cggatgtcgt cgaagctttc ccgcacggtt ctccgagggg
     1021 agacactcgc gacgtaacgc gtcgcgaatc accgtgctgc gtatggaccc atcagggagg
     1081 ttaccgtatg aaactcgcgt gtcaggaagg tatggcaccg ggaacaacgc tcgaagaaaa
     1141 gctcgccaac atcgaacaag caggatacga aggcgtcgag ctctggggag gcggtctcgt
     1201 tgatcgcctc gaggaagtca agcgcgcgac gaagaaggcg tcggtaccgg ttaccaccat
     1261 ctgcgccgga taccgcggca cgccgctgtc gaatgatttg gctacccgta agcaggcagc
     1321 cgacgacatc gtaatgctcc tcgacgccgc aggcgagctc ggcgcaacgg ggctcatctt
     1381 cgttccgata ttcgggaaag cgcaaatcaa cgatctcagc ccatggaaaa gcgccgaaga
     1441 gttggaacgc gaactcctcg tcgagctgct gggcgattgg gccgaacatg cacggaaagc
     1501 gggcacgctg ctcctgcttg agccgctcaa ccggtacgaa acgcatctca tcaaaacatt
     1561 gcgcgacgcc gtgcaaatct gcgagcaggt gaacaacccc aaaggcctca agatgatggc
     1621 tgacttcttc cacatgtcga tcgaagaacg ggatattccc acgtcgatcg gtgccgccgg
     1681 ggactggatc gcgcatatgc atctcgccga cagcacgcgc gaattgcccg gatacggtca
     1741 taccgacttt gcggccggct tcgcggcgtt acgcgaaatc ggtttcgacg gctatatggc
     1801 gctcgaatgc ggcatcccgg gcgacgataa ac
//
LOCUS       DSCC01000256            1895 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1041470, whole genome shotgun sequence.
ACCESSION   DSCC01000256 DSCC01000000
VERSION     DSCC01000256.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1041470"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..538)
                     /locus_tag="ENN56_03145"
     CDS             complement(<1..538)
                     /locus_tag="ENN56_03145"
                     /inference="COORDINATES: protein motif:HMM:PF05833.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74515.1"
                     /translation="MPEPTSDLVWLTCLLRELRTDFADRAVRLRRHTERPWVWIDGGV
                     DRSWIADMTPGASRVRRIRPDDDDAPTEWRLWGGRRTDGAQITDGTALGGDRVLSLRL
                     DWMTRLGESAASDLVIEFGGRHTNALVVTVPDGVIVDVLRPVSGKVNRYRELLPNRIY
                     APPPRLNRASLLDPWPWDA"
     regulatory      730..862
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            951..>1895
                     /gene="ribD"
                     /locus_tag="ENN56_03150"
     CDS             951..>1895
                     /gene="ribD"
                     /locus_tag="ENN56_03150"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDS74516.1"
                     /translation="MTLTSSTTTVPQEPSDFLAEAERWMGMALDLAAKARGYTSPNPM
                     VGCVVVRDGDIVGAGYHERAGLPHAEAVALDAAGDAARGADLYVTLEPCSHYGRTPPC
                     TERIIRAGVRRVICAHLDPDPRVNGRGVEQLRNAGIDVIVGPGADAARRLNEQYLYWK
                     RTGLSWITAKWAQTLDGRIATRTGSSQWITGEAARREAHRQRSYHDAVLVGIGTVLAD
                     DPRLDVRMVDGHQPWHIVLDPTLEIPLDACVIVPERTTVFTGQRIDDEKRRMLQDRGV
                     EVTSVDADRDRLDPAAVRSALGAKPFMSVFVECGPTEVT"
BASE COUNT          369 a          580 c          614 g          332 t
ORIGIN      
        1 cggcatccca cggccatgga tcgaggagcg aagcgcgatt caaccgtggc ggtggggcgt
       61 agatgcggtt cggcagaagc tcccggtacc gattcacctt gccactgacg ggtctgagca
      121 cgtcgacgat gacgccgtcc gggaccgtga cgacgagtgc gttcgtatga cgaccgccga
      181 actctatgac aaggtcgctc gctgcactct cgccaagtcg cgtcatccaa tcgaggcgga
      241 gcgagagcac acggtcgcca ccaagcgcgg tcccgtcggt gatttgcgcg ccgtcggtgc
      301 gacgtcctcc ccagagtctc cactccgtag gcgcgtcatc atcgtccgga cggatacgtc
      361 ggaccctgct cgcgccgggg gtcatatcgg cgatccatga ccgatccacg ccaccgtcga
      421 tccataccca tggacgttcg gtgtgacgcc gcaatcgtac ggcgcgatcg gcgaaatccg
      481 tgcggagttc gcgaagaaga cacgtcaacc agacgaggtc cgatgtcggt tccggcatgt
      541 gcgcctcatc gtgtcaaacg cgtcggatga gatgagaccg cgtggcacag ttgcgccacg
      601 agacgcggtc cgagggtcat agaaagaacc gaaaacaacc gattaccggc aaggatcggc
      661 atgcggtcgg agcgtcgaaa ctgttgactg aacgagatat gcgcggtaca tttactcttt
      721 catagcgcac tgttcttccg gggcggggtg aaagtcccga ccggcggtga tagcccgcga
      781 gaaagcgaac gtcacaccgt tcgctttccg agccggtgcg attccggcgc cgacggtata
      841 gtccggatgg gaggaggaac ggcgacgata ccaatacgcg cattctctcc ccgaatccgc
      901 ctcgggagtc gcgttccgtg tgtgccatgc ggcgataaga ctccgtacca atgacgctga
      961 catcatcgac tactactgta ccgcaagaac cgtcggattt tctcgcagaa gccgaacgct
     1021 ggatgggtat ggcgctcgat ctcgcggcga aagcgcgcgg atacacgtcg cccaacccga
     1081 tggtcggctg cgtcgtcgtg cgtgacggtg acattgtcgg tgcgggatac catgagcgcg
     1141 ccggcctgcc ccatgcggag gccgtcgcct tggacgcggc gggcgacgcg gcgcgcggtg
     1201 cggacctcta cgttacactc gaaccgtgct cgcattacgg ccgcacgccg ccatgcacgg
     1261 agcgcatcat ccgagccgga gtgcgtcgcg tgatctgtgc acacctcgat ccggacccac
     1321 gcgtcaacgg acgcggcgtc gagcagctcc gtaatgccgg tatcgacgtg atcgtcggac
     1381 cgggcgcaga tgcggcgcga cgactcaacg agcagtatct ctattggaaa cgaaccggtc
     1441 tctcgtggat taccgcgaaa tgggcgcaga cgctcgacgg tcggattgcg acacggaccg
     1501 gcagcagcca gtggattacc ggcgaggcgg caaggcgtga agcgcatcgg caacggtcgt
     1561 atcacgacgc ggtgcttgtc ggcattggca cggtgctcgc cgacgatccg cgactcgatg
     1621 tgcgcatggt tgacgggcat cagccgtggc acatcgttct cgatccgact ctggagatac
     1681 cgctggatgc ttgcgttatc gtccccgaac gcacaacggt gttcaccgga cagcgtatcg
     1741 acgatgaaaa acgccgaatg ttgcaggatc gcggcgtaga agttacttcc gtcgacgccg
     1801 atcgcgaccg gctcgacccc gcagccgtgc ggagcgcgct cggtgcgaag ccgttcatga
     1861 gcgtgtttgt tgaatgcgga ccgacggagg tgacg
//
LOCUS       DSCC01000257            1967 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039357, whole genome shotgun sequence.
ACCESSION   DSCC01000257 DSCC01000000
VERSION     DSCC01000257.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039357"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            2..889
                     /locus_tag="ENN56_03155"
     CDS             2..889
                     /locus_tag="ENN56_03155"
                     /inference="COORDINATES: protein motif:HMM:PF00924.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDS74517.1"
                     /translation="MTFDDLSTAVSEIWNLKLLSVGEGTLTVGLILSLFLGLIIVWII
                     AGFAQRLLVSRMLKRTHLDDGSKSALGRLGYYIILVLGVFLVLDTVGLKISSLAFLGG
                     LIGIGIGFGMQNIASNFISGLILLLERPIRAGDVVDINGDMGVVEDIKLRTTHVRTFN
                     NILLLVPNADLISTVVKNWSSEDMKVRGDVTVGVSYNSDPRKVEEILLRLAQEHENTM
                     EDPASTVAFLDFGNSSLDFVVRFWVPQPSLRVGTESDLRYRIFEAFKQEGIEIPFPQH
                     DLHIRASDAALPIENRNAS"
     gene            1127..1483
                     /locus_tag="ENN56_03160"
     CDS             1127..1483
                     /locus_tag="ENN56_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74518.1"
                     /translation="MRRVVVAIVILAALVITGILASSTNAEEPTRVRITNGLSRWSIH
                     YVYISSTSSSSWGDDQLNSDQVLSPGASETWTLSAGNYDLRVKDEDGDMYTRRGVCVP
                     RGMLVEWRVTIDDLDR"
BASE COUNT          455 a          540 c          491 g          481 t
ORIGIN      
        1 aatgacgttt gatgacctca gcacagcagt atcggaaatc tggaacctca aacttctcag
       61 cgtcggtgaa ggcacattga ctgtcggcct tattctgtcg ctgttcctcg gactgatcat
      121 cgtctggatc atcgccggat ttgcccaacg gctgctcgta agccgcatgc tcaagcggac
      181 ccatctcgat gacggaagca aatccgcgct tggacgactc gggtactaca tcattctcgt
      241 actcggtgtc tttctcgtac tcgacacagt cggcttaaaa atctcgtcac ttgcctttct
      301 cggtggcttg attggtattg gtatcggttt tggtatgcag aacatcgcat cgaattttat
      361 cagtggcttg atactcctgc tcgaacgtcc cattcgcgcc ggcgatgttg ttgacataaa
      421 cggcgacatg ggtgttgtcg aagacatcaa gcttcgtacc acccacgttc gtacgttcaa
      481 caacatcctt ctccttgtgc caaacgccga tctgattagt actgtcgtga aaaactggtc
      541 atcggaggac atgaaagtcc gtggtgacgt gactgtcggt gtgtcgtaca atagcgatcc
      601 gagaaaagtc gaggaaattc tcctacgtct tgcgcaggaa cacgaaaaca ccatggaaga
      661 tccggcttca acggtagcgt ttttagactt cggcaactct tctctcgact tcgtggtgcg
      721 tttttgggtg ccacagccat cgcttcgcgt aggcacggaa agtgatcttc ggtaccgcat
      781 tttcgaggca ttcaagcaag aaggtataga gattccgttc ccgcagcatg accttcacat
      841 ccgtgcaagt gatgccgcgt tgccgatcga gaaccgcaac gcgtcataat tcggaacgac
      901 tgataagctc ccttatgtat agaacggcgc agcgatgtag cactttttcg ctgcgccgtt
      961 tatattctca gtaaatgaat tatcagtatc gcggcttacc gagcgctctc ctccttgctt
     1021 tggtattcgt ttcacgatgt ttataaacga tttacgtgcg ctttcggtaa tagtgcgaac
     1081 cgtcatttga acgattcgcg tagcttcaaa aggggggggt aatacgatgc gcagagtagt
     1141 cgtagcgatt gtgatccttg cagcattagt catcacaggg atactcgcat cgagcactaa
     1201 cgctgaggaa cccacacgag tccgcattac gaacggtctg agtcggtggt cgatacacta
     1261 cgtgtacatc tcatcgacct ccagtagcag ttggggagac gatcagctaa actccgacca
     1321 ggttctctca cccggcgctt ccgagacgtg gaccctctct gcgggcaact acgatctccg
     1381 cgtaaaagac gaagatggcg acatgtatac ccgtcgtggc gtctgtgttc cgagaggaat
     1441 gctcgtcgaa tggcgcgtga caatcgacga tctcgataga tagaacaacg tcgcggtaca
     1501 gaaaaagcgg gaccgttctc gtctaacggt cccgcttcgt acgtctggtt cgttttcgct
     1561 atacgcgctg ttctcgccgc cacatccagt gtttctcgga aaccgaagcc cagacactgg
     1621 gaaggtgcgc ctcgtaaagc cgcgtcagat gcgcaaccgg attccagcgt cgcacgtttt
     1681 tgatcgcacg aagcacctca tccgcaccac tttccccgta cgaatcgagt agcgcacggt
     1741 tgtcctcaag cgtcgcaacg agctccgacc aacgactcat aatgccgttc cacgcctcaa
     1801 cgtaagctga ctcataccgt acgaacatat ccaatgccgt acgttcccgt ttcgccggat
     1861 cgtccggaag tgcacgaccg acgataaacg cgaagatcgg ttcgtcatat cgcccggccg
     1921 acgggacata gtgaaacaac tcaagtccgt ctcgaacgac aatcttc
//
LOCUS       DSCC01000258            2321 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031386, whole genome shotgun sequence.
ACCESSION   DSCC01000258 DSCC01000000
VERSION     DSCC01000258.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031386"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..976
                     /locus_tag="ENN56_03165"
     CDS             <1..976
                     /locus_tag="ENN56_03165"
                     /inference="COORDINATES: protein motif:HMM:PF02826.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDS74519.1"
                     /translation="HMINAETLAQTKKGVRIINCARGGIIDDDALLAALQSGHVAGAA
                     LDVFHTEPPDMDAPLIQHDHVIVTPHLGASTTEAQQLVAEQIAELIADALVNGNIRNA
                     VNMFPVDRVTYERASPYIDLAEKLGALQAQIIDGQPVALTVEYYGDALEHPTGLLTSA
                     VLMGLMRELESGTVNLVNAPMLAQERGIRVDELRTTGQEDYSSLITVKLTSETESGEE
                     QTRSLAGSVFGEDSERIVRIDGYTCDLIPEGHVIIVWNQDVPGVIGEVASTLGKHGVN
                     IAFGSFSREHPGGRALTAYMLDSEVDDATLRHLDSLDAVLRVKYAHLR"
     gene            1203..1652
                     /gene="nrdR"
                     /locus_tag="ENN56_03170"
     CDS             1203..1652
                     /gene="nrdR"
                     /locus_tag="ENN56_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDS74520.1"
                     /translation="MKCPFCGKLEDKVVDSRASRDGATIRRRRECLSCSERFTTYETV
                     EATPITVVKRDGRREPYDREKVLRGVRLAAKKRPVGDDQLERLVDDIELELFARQERE
                     VGAAEIGEMVLSRLEQVDTVAYIRFASVYKDFKTTGAFLDELKHLKT"
     gene            1661..1746
                     /locus_tag="ENN56_03175"
     tRNA            1661..1746
                     /locus_tag="ENN56_03175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:1695..1697,aa:Ser,seq:tga)
     gene            complement(1761..>2321)
                     /locus_tag="ENN56_03180"
     CDS             complement(1761..>2321)
                     /locus_tag="ENN56_03180"
                     /inference="COORDINATES: protein motif:HMM:PF01228.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDS74521.1"
                     /translation="DVKADELAADLSEFIFAEMLRRRTWRKNADGEGGPFVPSCLMFT
                     TYAHFGRQVGATPDGRRANEPIADSAGPYQGRDRSGPTAMLRSVTRLDQQHAPGTLVV
                     NIRLGKSHLVGAEQRAKTRALLRTYFDMGGLQLQVNVVDQELLRDAVAHPDRYADLIV
                     RIGGYSEYWSNLEPALRESVLLRTEH"
BASE COUNT          483 a          673 c          722 g          443 t
ORIGIN      
        1 ccatatgatc aatgccgaaa cattggcgca gacgaagaag ggcgttcgta tcatcaactg
       61 cgcgcgcggc gggattatcg atgatgacgc gctcttggca gcgcttcaat cgggtcacgt
      121 cgctggtgcg gctctcgatg tattccacac ggaacctccg gatatggatg ctccgcttat
      181 ccagcacgat cacgtcattg tcacgccgca tctcggtgcg tcgacgacgg aagcgcagca
      241 actcgtcgcg gaacagatcg cggaactcat cgccgacgca ctcgtaaacg gaaatatccg
      301 gaacgccgtc aacatgttcc cggtcgaccg agtaacgtac gaacgcgcga gcccgtatat
      361 cgacctcgcc gagaaactcg gtgcgttgca ggcgcagatc atcgacggtc aacccgtcgc
      421 gctgacagtg gagtactatg gcgacgcgct cgagcatccg acgggtttgc tcacgagcgc
      481 ggtgctcatg gggctgatgc gcgagttgga gagtggcacc gtcaatctcg tcaacgcgcc
      541 gatgcttgca caggaacggg gtatccgcgt cgatgagctg cgtacgacgg gtcaggaaga
      601 ctatagcagc ctgattacgg tgaagctaac gtccgagacg gagtccggcg aagaacagac
      661 acgctcgctc gcgggcagcg tgttcggtga ggattcggag cgcattgtgc gcatcgacgg
      721 gtacacgtgc gatctcattc cggaagggca cgtcatcatc gtctggaacc aggacgtgcc
      781 gggcgttatc ggggaggttg catcgacact cgggaagcac ggtgtgaaca tcgcattcgg
      841 atcattctcg cgagagcatc cgggaggccg cgcactgacg gcgtatatgc tcgatagtga
      901 ggtcgacgac gcaacacttc gccacctcga ctcgctcgat gccgttctcc gcgtgaaata
      961 cgcgcatctt cgctaatcgg atattcgggc atcgatatcg tggagaggcg agcctccggt
     1021 tcgcttctcc acgttcgtta cggggaagcg agtattcgtc ccaacggcct tccgacgggc
     1081 aatcccttgc gccatgcagg gtaatctgca atattcccta cgatatgccc ccggtgtcga
     1141 ctgtcgcttc gtgcgacggg tcacgttgga cgtattggca caggtgaaag aacgatatac
     1201 cgatgaagtg cccgttttgc ggaaagctcg aagacaaggt cgttgattcc cgcgcgagcc
     1261 gcgatggcgc gacgattcga cggcgtcgcg aatgcctctc gtgttcggag cgattcacga
     1321 cgtatgagac ggtcgaagcg acgccgatca cggtggtgaa gcgcgacggg cgccgcgagc
     1381 cgtatgatcg cgagaaggta ttgcgcggcg tgcgattggc ggcgaaaaaa cgtcccgtcg
     1441 gtgacgatca gttggaacgg ctcgtagacg acatcgagtt ggagttgttc gcacggcagg
     1501 agcgcgaagt cggcgcggcg gagatcggcg agatggttct ctcgcggttg gagcaagtgg
     1561 atacggtggc gtatatccgg ttcgcgtcgg tgtataagga cttcaagacg actggcgcgt
     1621 tcttagacga gttgaagcac ctcaaaacgt aacgccccgc ggaggggtgg ctgagtggtc
     1681 gaaagcaccg gtcttgaaaa ccggcaaccg aaaggttcgt gggttcgaat cccaccccct
     1741 ccgccagaat tactcccaac ctaatgctcc gtgcgcagca gcaccgactc gcgcagggcc
     1801 ggctcgaggt tggaccaata ctccgaatat ccaccgatgc gcacgatgag atccgcgtac
     1861 cgatccggat gtgcaacggc gtcgcgaagc aactcctgat cgacgacgtt aacttgaagc
     1921 tgtaatccac ccatgtcgaa atacgtccgc aggagcgcgc gtgtctttgc ccgttgctcc
     1981 gcacccacaa gatgagactt ccccagtcgg atgttcacca cgagcgtgcc gggcgcgtgc
     2041 tgctgatcga ggcgcgtaac actgcggagc atggccgtag gacccgatcg gtcccggcct
     2101 tgatacggtc ccgccgagtc ggcgatcggt tcatttgcac gccgtccatc cggagtcgcg
     2161 ccgacctgcc gaccgaagtg cgcatacgtg gtgaacatca agcacgacgg aacgaacgga
     2221 ccgccctcgc cgtcagcgtt tttgcgccag gtgcggcgtc tgagcatttc cgcgaaaatg
     2281 aactccgaaa ggtccgctgc cagttcgtcc gccttcacat c
//
LOCUS       DSCC01000259            1823 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043746, whole genome shotgun sequence.
ACCESSION   DSCC01000259 DSCC01000000
VERSION     DSCC01000259.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043746"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..322
                     /locus_tag="ENN56_03185"
     CDS             <1..322
                     /locus_tag="ENN56_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDS74522.1"
                     /translation="GQDEGAFEREEAEKSVLDTKVEELDLSMRSINCLRAAGIMSVEE
                     LVVKSESDMLKYRNFGRKSLIELGEKLEEHGLRFGMTEEEVEAVRTQNAPATEPTDEK
                     EVEQ"
     gene            319..792
                     /locus_tag="ENN56_03190"
     CDS             319..792
                     /locus_tag="ENN56_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDS74523.1"
                     /translation="MRHRVKGRRLGRTTSHRKAMLANLATSLIEHGGINTTDAKAKEL
                     RPFAEKLVTLGKRGDLTARRRAMQLIRDKRAVKRLFDDWAPRFQTRTVDGAEQEWNGG
                     YVRILKLGRRKGDAAEISMIEFVTGDAVAPEKECPAPAIEAKPSEKIEEKNEGGE"
     gene            944..>1823
                     /locus_tag="ENN56_03195"
     CDS             944..>1823
                     /locus_tag="ENN56_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDS74524.1"
                     /translation="MAERTRQKLERGVLVGGVIERLHTDGRGVMTALNGAGDEKDVVV
                     RGAWPGDEIDAQIRTKRKGRVEAETVAVIRRATPRRRPPCLHVWECGGCRWQHWQHVD
                     QLVQKRRLVEDAFTAEMPGREPEVRDTLRVSDEFTYRNKMEFTFGRVNFGPDAGQLVL
                     GLHRSGRFDWVFDVVSCHLASEPVNRVLSWVRDWAKRHRLTPYDQRRHDGLLRHLVVR
                     EARTTGEVMVNLVVALDDFAALDDFAPGLIEAVPTAASVLVSVNTRRGDTAIAESTRM
                     LAGKPTITERIGDIEIE"
BASE COUNT          418 a          483 c          601 g          321 t
ORIGIN      
        1 agggcaggac gaaggtgcat tcgagcgcga agaggccgag aagagcgttc tcgacaccaa
       61 ggttgaggag ctcgatctct ctatgcggtc gatcaactgc ctgcgtgccg cgggtatcat
      121 gtcggtcgaa gagcttgtag tcaaatccga gagcgacatg ctgaaatatc ggaacttcgg
      181 tcggaagtcg ctgatagagc ttggcgaaaa gcttgaagag catggcctgc ggtttggcat
      241 gacggaagaa gaagtagagg ccgtacggac gcaaaatgct ccggcgaccg aacctaccga
      301 cgagaaggaa gtcgagcaat gaggcaccgc gtaaaaggtc gtcgccttgg acggaccaca
      361 tcacaccgga aggcgatgct cgccaacctt gcgacgtcgc tcatcgaaca cggcgggatc
      421 aacactaccg acgcgaaggc caaggaactg cgtccgttcg ccgagaagct cgttacgttg
      481 ggcaagcggg gcgatctcac cgcccgacgt cgcgcgatgc agctcatccg agacaagcgc
      541 gcagtaaagc gtctgttcga tgattgggcg ccacgattcc agacgcgcac cgtagacggc
      601 gccgagcagg aatggaacgg cggatacgtc cgcattctga agctcggtcg tcgcaagggc
      661 gatgccgcag aaatatcgat gatcgaattc gttaccggtg acgcggtcgc gccggagaag
      721 gagtgtccgg cacccgcgat tgaagcgaaa ccgagcgaga agatcgaaga gaagaacgag
      781 ggcggcgaat aggccggccc tcctccatac cgtcgaattg catacactcg gcggagaacg
      841 aaattgagcg gagcggaatc ggcggtggct gataccgttc cgctctttta cgtatcgagg
      901 aagctgaccc gcgcatctga cggagcacgc gagaacggtt gacatggccg aacgaactcg
      961 acagaaactc gaacgcggcg tgctggtcgg tggcgtcatc gaacggttgc acacggacgg
     1021 tcgcggcgta atgaccgcgc tgaacggtgc gggcgacgag aaggatgtcg tagtccgtgg
     1081 tgcttggccc ggtgatgaga tcgatgccca gattcgcacg aagcggaagg gtcgcgtcga
     1141 agcggaaaca gtcgcggtca tcaggcgggc gacgccgcga cgccgtccgc cgtgtctgca
     1201 cgtgtgggag tgtggcggtt gtcggtggca gcactggcaa cacgtcgatc agctcgtgca
     1261 gaaacgtcga cttgtcgaag acgcttttac ggcagagatg ccgggtcgag aacccgaggt
     1321 acgtgataca cttcgcgtga gcgatgagtt tacgtatcga aacaagatgg agttcacatt
     1381 cggccgcgta aattttggtc ccgacgcggg ccaactggta ctcggactcc atcgatccgg
     1441 tcgattcgat tgggtgttcg acgtagtttc ttgtcacctc gcgagcgaac cggtaaaccg
     1501 agtcttatcg tgggtccgtg actgggcaaa acgacacagg ttgacaccct acgatcagcg
     1561 ccgacacgac ggactgctcc gtcatctcgt cgtacgggaa gcacgtacca ccggtgaggt
     1621 gatggtgaat ctcgtcgttg cgctcgatga tttcgccgcg ctcgatgatt tcgcgcccgg
     1681 gttgatcgaa gcggtgccaa ctgcggcgag tgtgctggtg agcgtgaata cacggcgcgg
     1741 cgacacggcg attgcagaga gcacacgcat gcttgcaggt aagccgacga taaccgagcg
     1801 tatcggcgat atcgaaatag agc
//
LOCUS       DSCC01000260            2172 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034411, whole genome shotgun sequence.
ACCESSION   DSCC01000260 DSCC01000000
VERSION     DSCC01000260.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034411"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..513)
                     /locus_tag="ENN56_03200"
     CDS             complement(<1..513)
                     /locus_tag="ENN56_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74525.1"
                     /translation="MQNLSAGFGLIEITPLVGTPLIGQLHARYSTGVHDPLFARVLVL
                     RAGENDSNAVMLIVLDVCKVPEYDADRIRDAITAQLPIARDRIWVFATHTHTGPALHT
                     SVETPRAEKYAAWLHGEVARAAATAWEDLAPATVKFVQTDVDGVGFVRRYRMKDGSIR
                     TNPGVGNPDIV"
     gene            complement(613..2109)
                     /locus_tag="ENN56_03205"
     CDS             complement(613..2109)
                     /locus_tag="ENN56_03205"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS74526.1"
                     /translation="MDDTTVSPQPALDEAPHQTSSTSDNAPRSILSYFRYRPTLPVEE
                     ITPRDRHNGMQKVILAWVAGSIFFEGIGGTPFVGMFRMLGATPAWIGLLVALPSLASL
                     ATPIGSWIMARTGSRKRFFLRFSYTGRAMWLVVIFAGLILPHGSSATLTFLFGCILFG
                     RLAMALTQPAWWSWVSDLIPAERFGEYWGRRQMWGRISGAVGLLILNWFLGADPTYGR
                     FVFYFTIVSLFGLFDIFIHRGVTGIRVKVNREEPPQFHRLFIEPIRDRKFRPLLIFIG
                     CFAFANQLGGGMLHLMFLEDFQLTYFEIALYMPGLLGIMWVIGSRFWGRLVDNLHDGR
                     RIVFIVCSVVVATHVLIFVFTGPRQHSLIATALALGGISWSGYHIALTALIIGMSPSH
                     ARGQYFAAYAVFFGIGDMLGAISAGQLAELFAGLNYELGPLHLTQFRTPYLISAGLRV
                     SCLFLLPFIREPDSRPIGVFVRRIFSLNPFHRGTWVYVRSKLDRPRSM"
BASE COUNT          504 a          618 c          692 g          358 t
ORIGIN      
        1 gacgatatcc ggattaccga cgccgggatt cgtgcgaatg cttccatcct tcatacggta
       61 tcgccggacg aatccgacgc cgtcgacatc cgtttgaacg aacttcaccg tggcgggcgc
      121 gaggtcttcc cacgccgtcg cagccgcgcg tgcgacttca ccgtggagcc acgccgcgta
      181 tttttcggcg cgcggtgttt cgactgaggt gtggagcgcc gggccggtgt gggtatgcgt
      241 cgcgaagacc cagatgcggt cgcgcgcgat gggaagttgc gctgttatgg cgtcgcgaat
      301 gcggtccgcg tcgtactccg gaactttaca cacgtcgagc acgatgagca tcacggcgtt
      361 cgaatcgttt tcgccggcgc gcaggaccag tacgcgcgca aagagcggat cgtgcacacc
      421 ggttgaatac cgcgcgtgga gctgcccgat gagcggtgtg ccgacgagcg gcgttatttc
      481 gataagtccg aatccggcgg agaggttctg catgccgata actccttcgt gaaatcgtcg
      541 acgatagtgt gtagaagctc acgcgaagcc gcatggaacg caaaggggac ggtgtaaacg
      601 aacccgtatt tttcacatcg accggggacg atcgagctta ctgcgcacgt acacccatgt
      661 tccccgatgg aaggggttca gcgaaaagat tcgacgtacg aaaacgccga tcggacgtga
      721 gtccggttcg cgaatgaacg gaaggaggaa cagacaggag acgcgcaatc ccgccgagat
      781 caggtacggc gtgcggaatt gcgtgaggtg cagcgggccg agctcgtaat tcagccccgc
      841 aaagagttcc gcgagttgcc ccgcgctgat cgcgcccagc atgtcgccga tgccgaagaa
      901 caccgcatac gcggcgaaat actgtccgcg ggcgtgggag ggcgacatac caatgatcaa
      961 cgccgtcagc gcgatatggt agcccgacca actgataccg ccgagtgcaa gcgcggtggc
     1021 gatgaggcta tgctggcggg gcccggtgaa cacgaagatc agtacatgcg tcgcgacgac
     1081 cacggagcag acgatgaaaa cgatgcggcg accatcatgg agattgtcga cgagccgtcc
     1141 ccagaaacgc gatccgatga cccacatgat tcccagcaga cccggcatgt acagcgcgat
     1201 ctcaaagtag gtaagctgga aatcttcgag gaacatcagg tggagcatac cgccgccgag
     1261 ctggttggcg aacgcgaagc atccaatgaa gatgagcagc ggacgaaact tccgatcgcg
     1321 gatcggctcg atgaacaggc ggtggaactg cggcggttct tctcgattga ccttcacgcg
     1381 gatgccggtc acgccgcggt ggataaagat atcgaacaac ccgaacagcg atacgatcgt
     1441 gaagtagaac acgaaccggc cgtaggttgg gtccgcgccg agaaaccagt tcagaatgag
     1501 cagaccaacg gcgccgctga tgcgtcccca catctgccgg cggccccagt attcaccaaa
     1561 acgctctgcc ggaatgagat cgctcaccca cgaccaccac gcaggttgcg taagcgccat
     1621 ggccaaacga ccgaacagga tgcaaccgaa gagaaacgta agcgtcgccg aactgccgtg
     1681 tggcaggatg agtcccgcga agatgacgac cagccacatg gcgcgaccgg tgtacgaaaa
     1741 ccgcagaaag aagcgtttgc gggacccggt ccgtgccata atccacgaac cgattggtgt
     1801 cgcgagcgac gcaagcgacg ggagcgcaac gaggaggccg atccacgcgg gcgttgcgcc
     1861 gagcatgcgg aacatgccga cgaacggggt tccgccgata ccttcgaaga agatcgatcc
     1921 ggcaacccac gcgagaatga ccttctgcat cccattgtgg cgatcacgtg gcgtaatctc
     1981 ttccactggg agcgtcgggc gataccgaaa gtacgacagg atcgagcgcg gcgcgttgtc
     2041 actcgtcgac gatgtctggt ggggcgcttc atcgagcgca ggttgcggag aaaccgttgt
     2101 atcgtccatc aacgcgtgag gtacggaacg tcgccacgaa tcagagtgtg gagtcagagg
     2161 tagatatggg cc
//
LOCUS       DSCC01000261            2411 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029882, whole genome shotgun sequence.
ACCESSION   DSCC01000261 DSCC01000000
VERSION     DSCC01000261.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029882"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..482
                     /locus_tag="ENN56_03210"
     CDS             <1..482
                     /locus_tag="ENN56_03210"
                     /inference="COORDINATES: protein motif:HMM:PF13320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDS74527.1"
                     /translation="PGEYAHTQRILDTAPIVWTYACGEGAKDRRRMEYYWAPIWRGAQ
                     LGITGIGFWSYAGRTIDMWQGPVSYGCDWELVYPGDGTVVPSHRWQGLRIGIEDRMRL
                     ELVTEMAKHARVDGDPIRAKELESLRDACIRRVVESGHDEGVAATVRKELRSVLLGE"
     gene            487..1797
                     /locus_tag="ENN56_03215"
     CDS             487..1797
                     /locus_tag="ENN56_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74528.1"
                     /translation="MPDNRLTAIIVGAGHRAVGYARKGRDSIRVVGIADPRDQRREMI
                     ADEFGIPVKHQFRTAEDLARVPKFADVVINGTMDHQHVPTSLPLIRAGYDILLEKPFA
                     VNEEEMWELVEAAREYRRTVLICHVLRYAPFYAAIRQKVVDDAIGRILNVQTVEHVSS
                     HHMDVAFIRGKWAKKSLCGSSMLMAKCCHDLDIIAWMKSGTFPVRVASFGGNYQFKPE
                     DAPEGAGERCLVDCPIESDCLYSARKIYLDHPNRWSFYVWERFEGVENPTIEQKTESL
                     KTDNPHGRCVWKAGTEMVDHQSVVIEFSDGATATHNMVGGTAKALRSIHLIGTRGEIQ
                     GVFEESRFVVRSIAPERPENTIEEVVDLTVGGDMHGAFGGHGGGDERLVEDFVRVVRG
                     EQPSVSTTHIEDSVSGHLVGFSADRAMTENRVVELDATRIAALG"
     gene            1867..2097
                     /locus_tag="ENN56_03220"
     CDS             1867..2097
                     /locus_tag="ENN56_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74529.1"
                     /translation="MGLQKPRQFSYRPPERRKKQLADKEGEEFRPRIELPKAGDGRTS
                     LTSSGGMFSGGLRLLLMLIAIVGLALVFASLR"
BASE COUNT          545 a          642 c          727 g          497 t
ORIGIN      
        1 atccagggga gtacgcgcac acgcagcgaa tccttgatac cgcgccgata gtatggacgt
       61 atgcctgcgg cgagggcgca aaagatcgtc gccgtatgga gtattactgg gcgccgatct
      121 ggcgtggtgc gcaactcgga atcaccggta tcggattctg gtcctacgct ggtcgcacga
      181 tcgatatgtg gcagggacca gtgtcatacg gttgcgactg ggaacttgtc tatcccggcg
      241 atggaacggt agtaccgagt caccggtggc aaggcctccg tatcggtatc gaagaccgga
      301 tgcgtcttga actcgtcacc gagatggcga agcacgctcg agtagacggt gatccgattc
      361 gagcaaaaga actggaatcg ctccgagacg catgtatacg ccgtgtggtg gaaagtggac
      421 acgacgaagg cgtagcggct acggtgcgca aggaactccg ctcggtgctg ttgggggaat
      481 gagtcgatgc cggacaaccg attgacggcg atcattgtcg gtgcggggca tcgtgccgtt
      541 ggttacgcgc gaaaagggcg agattcgatc cgtgttgtcg gtatcgccga cccacgtgac
      601 caacgccgcg aaatgatcgc cgatgaattc ggtataccag ttaaacacca gttccgcacc
      661 gcggaagatc tcgcgcgtgt tccgaagttc gcagatgtcg tcatcaacgg tacaatggac
      721 caccagcacg tacccacttc gctaccgctg attcgcgccg gatacgacat tcttctcgaa
      781 aaaccgttcg ccgtaaacga agaggaaatg tgggaactcg tcgaagccgc gcgtgagtat
      841 cgccgtacgg tactcatatg ccacgtactt cgttacgcac cgttttacgc ggcgattcgg
      901 cagaaggtcg tcgacgacgc gatcggacgg atcctcaacg tgcagaccgt ggagcacgtg
      961 agttctcatc acatggacgt cgcgttcatt cgtgggaagt gggcaaagaa gagcctttgc
     1021 ggttcgtcga tgctcatggc gaagtgttgt cacgacctcg acatcatcgc gtggatgaag
     1081 agcggcacgt tccccgtgcg cgtggcgagc ttcggcggaa actaccaatt caagcccgaa
     1141 gacgcaccgg aaggcgcagg cgagcgctgc ctcgtagatt gcccgattga gtcggactgt
     1201 ctgtactccg cgcggaaaat ctacctcgat caccccaatc gatggagttt ctacgtctgg
     1261 gagcgttttg aaggagtcga gaacccgaca atcgaacaga aaactgagag cctcaagacc
     1321 gataatccgc acggtcgatg tgtgtggaag gccggtacgg aaatggtaga tcaccagtcc
     1381 gtcgttatcg agttctcaga tggcgcaaca gcgacgcaca atatggtagg cggcacagcg
     1441 aaggcgttgc ggagtatcca tctcattggg actcgcggcg agatacaagg cgtattcgaa
     1501 gaatcgcgat ttgtcgttcg gagtattgca ccggagcgac cggaaaacac catcgaagag
     1561 gtcgtcgatc tcacggtcgg tggagatatg cacggcgcat ttggtggtca cggtggtggt
     1621 gacgaacgcc tcgtcgaaga tttcgtcagg gtcgttcgcg gcgaacagcc gtcggtgtcg
     1681 accacgcaca tcgaggattc cgtcagcggc cacctcgtgg gctttagcgc cgaccgcgcg
     1741 atgaccgaaa atcgtgtcgt agaactcgac gctacgcgaa tagcggctct cggctgatgc
     1801 gggaacaccg ttcgcgcaca aagcgttatt acgggtacga gagttcttcg ataaaagggg
     1861 gtactcgtgg ggttgcaaaa accacgtcaa tttagctatc gaccgcctga acgccgaaag
     1921 aagcaactgg ccgataaaga aggtgaagag tttcgcccgc gtattgaact tccgaaggcg
     1981 ggcgacggaa gaacgagcct gaccagtagc ggtgggatgt tttccggcgg gcttaggctc
     2041 ctcctcatgc tgatcgcgat tgtcggtctt gcactcgtct tcgccagcct ccgttgaccc
     2101 ggttgccgat ttgtaaccac attctacacg gctcatccga cacttcgatg agccgtttcg
     2161 cgtgtacgaa acgaccgtgc acgacatcac gaaaaattgg cggagaaacg ctttcacgtc
     2221 gtttcaagac taggccgaca ccctaaggtg acgtgacact cagaattccg tatatttatc
     2281 gatcaatcgt tttgacggat tcgacgattg acggcgtctt ttctcgtttc ataaccgacg
     2341 cgaccgagga ttctgcgggc gcggcggtca tcgtctgaac tcacccccac aggccaccga
     2401 ttgttcgggt g
//
LOCUS       DSCC01000262            2453 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029223, whole genome shotgun sequence.
ACCESSION   DSCC01000262 DSCC01000000
VERSION     DSCC01000262.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029223"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..553
                     /locus_tag="ENN56_03225"
     CDS             <1..553
                     /locus_tag="ENN56_03225"
                     /inference="COORDINATES: protein motif:HMM:PF06251.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74530.1"
                     /translation="PSASYVIRADFDAEEIPDLEYKRLVDVPQASMRGEEYIYFRTRS
                     RQMFGVVDTDIAAALAEPGGSADVILRGGDVIRIEQQPVSVEVLGQVERPGMVNFEPG
                     KSYQQYIDQAGGLSWHARKRGIRLIKTSTGVWVKPDRDTVVEAGDIIFVPQREPIDYW
                     ALTKDIVGVTTQILTVYLLIVTR"
     gene            559..1719
                     /locus_tag="ENN56_03230"
     CDS             559..1719
                     /locus_tag="ENN56_03230"
                     /inference="COORDINATES: protein motif:HMM:PF02706.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74531.1"
                     /translation="MNEKQEKRSIDGYDDSSHLVNLAGLIWRRRKTVIGTITLIVGIT
                     IVALLIAKPQFTARATFMPPPDGSGGLASMLRDPINAILGRGGGTSMDRLVNFLDSRV
                     TRDLIVDKYDLRTYYEVDYIDEARKALANATDVSVSPEGLVRVDVTDKDRVLAAQIAN
                     EYVDIADSLYVIAQREYAGQMRRFLETRVEQNLRDLSLAEEAARAFSEQYGVVSLPNQ
                     VAALIDQLAQVEAQIRTLDMRIGAAREILGPNHVSVRELRVEREMAERERRKLLQSNG
                     ATTDPFLAFQDIPVRALEYARLEREIKIQSIIQELLFQQYEVAKLEEARNVSSLTRVD
                     TAIPPELKSWPPRTRIVLVTGIIAFVWAIFVAWFVERWPDYRRRIVESGGRT"
     gene            1732..>2453
                     /locus_tag="ENN56_03235"
     CDS             1732..>2453
                     /locus_tag="ENN56_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74532.1"
                     /translation="MNLRRLTNETIIYGIGRGIEGIAAFVVLPFIGRYLVPSVFGYAD
                     VISATTGLLYLTVGLNLHQALTRYYYDVDSGTLVTTHSLTFLGSGAVHLGIGAGIVLL
                     FRGTVMEPAAWIIAITSVIGQFTSEYITTIFRMRHEPLRYLSVVFVTQLSLCIATPVL
                     LVVFNAGINAIFIGKSVAFGSGILISVGWLIAIYRKRFDVGYLRRSIRFILPMVPGNI
                     ASWALMYAPRYFLIAAATAAQVG"
BASE COUNT          537 a          653 c          677 g          586 t
ORIGIN      
        1 accgagcgca tcctatgtca tccgagccga ttttgacgcg gaagaaatcc ctgatcttga
       61 atacaaacgt ctcgtcgatg tcccgcaagc gtcgatgaga ggcgaagaat acatttactt
      121 ccgcactcgc agtcgccaga tgttcggtgt cgtcgacacg gatatcgccg ccgcactcgc
      181 tgagccgggt ggatccgccg atgtcatttt acgtggcggc gacgttatcc gaatcgaaca
      241 gcaaccggtg agtgtcgaag tactcgggca agttgaacgt cccggcatgg tgaatttcga
      301 gcccggcaag tcgtatcaac agtacatcga ccaagctggc ggtctttcgt ggcatgcgcg
      361 aaaacgcgga atccgattga tcaagacgag tacgggcgtt tgggtaaagc ccgatcgtga
      421 tacggtcgtc gaggctggcg acatcatctt cgtgcctcaa cgcgaaccga tagattactg
      481 ggcgctcacg aaggatatcg ttggcgttac cacgcaaatt ctcactgtct acttgctcat
      541 cgtcacacgt tgagacctat gaacgagaaa caagaaaaac gcagtatcga cggttacgat
      601 gacagcagtc atctggtaaa tcttgccggc ttgatctgga ggcgtcgcaa aaccgttatc
      661 gggacgatta cccttatcgt cggaatcacg atcgttgcgc tcctcatcgc gaagccccaa
      721 tttactgcgc gcgccacgtt tatgccacca cctgacggtt cgggcggact cgcgtcaatg
      781 ctgcgcgatc cgatcaacgc aattctcggt cgaggtggcg gaacctccat ggatcggttg
      841 gtgaatttcc tcgacagtcg cgttacacgc gatctgatcg tcgacaagta cgatcttcga
      901 acatattacg aggtcgatta tatcgatgaa gcgcgaaaag cgcttgcaaa cgcaaccgat
      961 gtttccgtgt cgcccgaagg actcgttcgc gttgatgtta cggataagga cagagttctc
     1021 gctgcgcaga ttgcgaacga atacgttgat atcgccgaca gtctgtatgt aatcgcgcaa
     1081 cgtgagtacg cgggacaaat gcgccggttt ttggaaacac gcgtcgagca gaacctacga
     1141 gacttgtcgc tggccgaaga agcggcgcgc gcgttctccg agcagtacgg ggttgtgtcg
     1201 cttccgaatc aggtggcggc actgatcgac cagctcgccc aagtcgaagc gcagatccgg
     1261 accttggaca tgcgcatcgg cgcggcgcga gagatccttg gtccgaacca cgtatccgtg
     1321 cgggagctac gtgtcgagcg cgaaatggcc gagcgcgaac ggcgcaagct tcttcagtca
     1381 aacggagcaa ccaccgatcc gttcctcgcg tttcaagata ttcccgttcg agcgttggag
     1441 tatgcgcgtc tcgaacgtga gatcaagatt caatcgatta tacaggagct ccttttccag
     1501 caatacgagg tagcgaaact cgaagaagca cgtaatgtat cgtcgctcac ccgcgttgat
     1561 accgcgattc cccccgaatt gaaatcgtgg ccgccacgga cgcgcatcgt actcgtcacc
     1621 ggtatcattg cgtttgtctg ggcgattttc gtcgcatggt tcgttgagcg atggccggat
     1681 taccgtcgaa gaatcgtcga gtccggcgga cgcacctgag cggcgtgcgt aatgaatctt
     1741 cgccggctaa cgaatgaaac gatcatttat gggatcggaa ggggtataga agggatcgcg
     1801 gccttcgtgg tccttccgtt tatcggtcga tatctcgtcc cttccgtatt cggttatgcc
     1861 gatgttatct cggcaacaac cgggcttctc tatctgacgg ttggtctaaa tctccatcag
     1921 gcactaaccc gctactatta cgacgtcgat agcggaacac ttgttaccac ccattcattg
     1981 acatttctcg gcagtggcgc tgtccatctc ggaattggtg ccggaatcgt gttgttgttc
     2041 cgtggtacgg tgatggaacc tgcagcatgg attattgcca taacatccgt tattgggcag
     2101 ttcacttcgg agtatatcac gaccatcttc cggatgcgtc acgaaccgtt gcggtatctt
     2161 tccgtcgtgt ttgtaacgca actttcgttg tgtatcgcga cgcccgtact gctcgttgta
     2221 ttcaatgcgg gaataaatgc gatttttatc ggaaagtctg ttgcgttcgg ttccggaata
     2281 cttatcagcg tcggctggct aatagccatt taccggaagc gcttcgatgt tgggtatctt
     2341 agacgcagca tacgatttat cttgccgatg gtccctggaa acatcgcatc gtgggcactc
     2401 atgtatgcgc cacggtactt cctcattgcg gccgcgaccg cggcgcaggt ggg
//
LOCUS       DSCC01000263            2172 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034419, whole genome shotgun sequence.
ACCESSION   DSCC01000263 DSCC01000000
VERSION     DSCC01000263.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034419"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(185..1240)
                     /locus_tag="ENN56_03240"
     CDS             complement(185..1240)
                     /locus_tag="ENN56_03240"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74533.1"
                     /translation="MQGSRQRVFDMMEGRTPDRVPLYDLLRNDAVIKHFTGRDLTVEN
                     ADELLFEAFPKAIDTTRRVRLPEHPSDTRLPDGRRCVTKRWTSWTEHVKYASSEEYAE
                     VKRRELAGPWDRWTEDDQRDVEESLAAYRQKAARLGEDFFFFSGGPGIHLMGIYGEVG
                     LEHFCYYLADCPDVIDAQLEQRTVRAEMWLEHLPEDHGIVAVFSGDDIAYKGGPLLSP
                     AWFRENYFARFKRLIAAYHRRGILVNFHSDGNLMPILDDLVEAGIDMLNPIETAAGMD
                     IAEIHRRYPDLIMAGGIDVSTLLPFGTPDEVRDVTIRAIEDAEGKIMVGSSTELHNDV
                     PLANFLAMREAVLGYRL"
     gene            complement(1270..2172)
                     /locus_tag="ENN56_03245"
     CDS             complement(1270..2172)
                     /locus_tag="ENN56_03245"
                     /inference="COORDINATES: protein motif:HMM:PF13088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74534.1"
                     /translation="MYRTSADFGRTWSDRQVLIEAAREEGADFEKWNCPRVGQLNDGR
                     VYILCDRFPVDRGHEDEGSSTHVWWMRDDGTWDGPHDTGVFGIVPDRICELPSGRLLL
                     GTHWHNGETKRLTQTVAFSDDNGATWSDRVTVASSAELNLCEGSIIRLPGGELVCYMR
                     ENSGLGLPMYKSISADDGITWSEIAPTLMAGGHRAVAGLTASGAVLVTYRQTIGGTGR
                     SPQNTFAYRESVESARELDPRKQSGTILPIDHDRSPNRDNSYTGWVNLPDGRIFIVNY
                     LVDDAPRAQIRGYWVTETQFTLPV"
BASE COUNT          441 a          689 c          605 g          437 t
ORIGIN      
        1 cgttagccct tcgcccgacg tctccgagcg gaacgcgagg agacgcgcgc gaaatcgacg
       61 caccgcgaaa tggatatttc gctacgtatc cgccgtcccc tcgcgttccg ctcgaagccg
      121 ccccgggcga agacgcgcag cgtcttagcc ttaacgaacg cgagcgccag ctcgcgttcg
      181 cgccctacaa ccgatacccg agcacggcct cccgcatggc gaggaagttt gcgagtggga
      241 cgtcgttatg cagttccgtg ctcgatccta ccatgatctt cccttccgcg tcctcgatcg
      301 cgcgaatcgt cacgtcacgc acctcgtcag gcgtgccgaa cggcagaagc gtgctcacat
      361 cgatgccacc cgccatgatg aggtccggat accgccgatg aatctcggca atatccatcc
      421 ccgcagcggt ctcgatcgga ttcaacatgt caattcccgc ttcgacgaga tcgtccagaa
      481 tcggcataag gttgccgtcg gagtggaaat tcacgaggat cccgcgccta tggtatgcgg
      541 cgattaggcg tttgaatcgc gcgaagtagt tttcgcgaaa ccacgcggga ctgagtagcg
      601 gaccaccctt atacgcgata tcgtcaccac tgaaaaccgc gacgatgcca tgatcttccg
      661 gtaagtgctc caaccacatt tcggcccgaa cggtgcgttg ttcgagttgc gcatcgatga
      721 cgtcgggaca gtcggcaagg tagtaacaaa agtgctcgag cccgacctct ccgtatatcc
      781 ccatcagatg aattccgggg ccaccggaga aaaagaaaaa gtcttcgccg agccgcgccg
      841 ccttttgtcg atacgccgca agggattcct ccacatcgcg ctgatcatct tccgtccatc
      901 gatcccacgg acccgcgagc tcgcgccgct tgacctccgc gtattcctcg gatgacgcgt
      961 atttgacatg ctcggtccac gatgtccatc gtttcgtgac acaacgacga ccgtcgggaa
     1021 gtcgcgtgtc gctcggatgt tccgggagac gtacgcgtcg agtggtatcg atagccttcg
     1081 ggaacgcctc aaacagcagc tcgtcggcgt tctccacagt gagatcgcgt ccggtgaagt
     1141 gtttgataac cgcgtcgttt cggaggagat cgtagagcgg gacacggtca ggcgtccgac
     1201 cttccatcat gtcgaacaca cgttgtcgag agccttgcat tgaaaagtct ccgcgctgag
     1261 aaaagagtgt tataccggca gcgtaaactg tgtttcggtg acccagtatc cacgaatctg
     1321 cgcacgcggg gcatcgtcga caaggtaatt cacgataaag attcgtccgt cggggagatt
     1381 gacccagccg gtataactgt tgtcccgatt cggactccga tcatgatcga taggaaggat
     1441 cgtgccggat tgcttgcgcg ggtcgagctc acgtgcgctt tcgacggatt cgcgataggc
     1501 gaacgtattc tgcggtgaac gtcccgttcc accaatggtc tgacgatacg ttacgagcac
     1561 cgcgccactc gctgtcagac ccgcaaccgc gcgatgacca cccgccataa gtgtcggtgc
     1621 gatctcagac cacgtgatcc cgtcatccgc gctgatcgac ttgtacatcg gcagcccaag
     1681 ccctgagttc tcgcgcatgt agcatacgag ctcgccgccc ggcaatcgga tgatcgagcc
     1741 ttcgcagagg ttcagctccg cactcgacgc gacggtaacg cggtcgctcc acgttgcacc
     1801 gttgtcgtcg gagaacgcaa cggtttgcgt aaggcgcttc gtttcaccat tgtgccaatg
     1861 cgttccgagg aggaggcggc ccgacgggag ttcacagatc cggtccggca cgataccgaa
     1921 cacgccagta tcgtgcgggc cgtcccacgt gccgtcgtcg cgcatccacc agacgtgcgt
     1981 tgacgagccc tcgtcctcat gcccgcgatc taccggaaac ctgtcgcaca atatgtacac
     2041 gcgaccgtcg ttcagctgtc cgacgcgcgg gcaattccac ttctcgaaat ccgcaccttc
     2101 ctcgcgcgcg gcttcgatga gcacctgccg atcactccac gttcgcccga agtcggccga
     2161 tgtgcgatac ac
//
LOCUS       DSCC01000264            1917 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040802, whole genome shotgun sequence.
ACCESSION   DSCC01000264 DSCC01000000
VERSION     DSCC01000264.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040802"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..850
                     /locus_tag="ENN56_03250"
     CDS             <1..850
                     /locus_tag="ENN56_03250"
                     /inference="COORDINATES: protein motif:HMM:PF01547.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDS74535.1"
                     /translation="PDTSWTYDDFRRIAIALTKDHDGDGRIDQFGTSLAFSFLGSETF
                     LWAWGADVIDRKEFRSGFDTPEAHDAIEFLRQLVIVDNASPQAGQMPGIASEIAILTG
                     RLAMVPGPTYLMQNLRATEGMRWDVFHMPKGPTGIRATRASWDGIAIYSGSEKKDIAW
                     EYIKFVVSRKAQRYIGEANRAMPVRRQDVYDFFLKEDREQHDERFLEATEYAFLTPAV
                     TKFDAMDNATARWLDRIRTGSSTVEEALANLSAEYNQMLEADRQYFTEREAVKTLPKT
                     YSETTQ"
     gene            885..1214
                     /locus_tag="ENN56_03255"
     CDS             885..1214
                     /locus_tag="ENN56_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74536.1"
                     /translation="MKIFSLRDLRPNTDRRSFFTDGSPLAEGVNHFNVADVVFLNRGE
                     TWSAQPATGAERAVVVMQGSGRMTIDGVEHPVRAFDVIVIQSGEEWSIRGDDVDPAVI
                     LVLGAGA"
     gene            1216..>1917
                     /locus_tag="ENN56_03260"
     CDS             1216..>1917
                     /locus_tag="ENN56_03260"
                     /inference="COORDINATES: protein motif:HMM:PF01925.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDS74537.1"
                     /translation="MAFDLETLLLLEAIILLAAFIQGAVGFAFGMTSMGLAVFLIDAR
                     VASIIVSPLAATNVALALWSVRHAIRLRNVIPMVVGMVIGLPFGLLILLEGSTTIIRI
                     SVAALLIYVGTSRLLSNGRTRRPLHPWWGYVAGLFAGIVGGATNIGGPPLIAYVAHQP
                     WKPVVFKASLLTCFLVSSGLKTIMLGIEGSLDSQLLASVAVLLPAVVIGSQIGIAFFN
                     SVNQRLFGHLVSGLIV"
BASE COUNT          412 a          521 c          537 g          447 t
ORIGIN      
        1 ccccgataca tcgtggacgt acgatgattt ccgccgaatc gcaatcgcac tcacgaaaga
       61 ccacgatggc gatggtcgta tcgaccaatt cggcaccagt ctcgcattca gttttcttgg
      121 atcggagacg ttcctttggg cgtggggtgc tgacgtcatc gatcgcaagg agtttcgttc
      181 cggattcgat acgccggaag cgcatgacgc cattgagttt ctccgtcaac tcgtcatcgt
      241 agataatgcg agtccgcaag caggtcagat gccgggaatt gcatctgaga tcgccattct
      301 cactggtcgg cttgcgatgg ttcccggtcc gacgtatctc atgcaaaacc ttcgcgcaac
      361 tgaaggaatg cggtgggatg tattccacat gccaaaagga ccgacaggta tacgtgcgac
      421 acgagcatcg tgggacggaa ttgcgatcta cagcggctct gaaaagaagg atattgcgtg
      481 ggaatacatc aaatttgtcg tgagtcgtaa agcgcaacgc tacatcggtg aagcgaatcg
      541 cgcgatgccg gtgcgtcggc aggacgtgta tgacttcttc ctcaaagaag atcgtgaaca
      601 acatgacgag cggtttttag aagcgacgga atacgctttc ctcacacctg ccgtaacgaa
      661 gtttgacgcg atggataacg caacagccag atggcttgac cggattcgca ccggttcaag
      721 caccgtcgag gaagcgcttg cgaacctgag cgccgagtac aaccaaatgc tcgaagccga
      781 tcgccagtat ttcactgagc gagaagcggt caagacactt ccgaagacgt attccgagac
      841 aactcaatag cgccactcca cgaaaatcca acggatatac cccaatgaaa atcttttcgc
      901 ttcgagacct tcgtccaaac accgaccgac gaagtttttt tactgatgga tcgccacttg
      961 ccgagggagt caaccatttc aacgtcgccg atgtggtttt tctcaatcgc ggagagacgt
     1021 ggagtgccca gccggcaacc ggtgccgagc gagccgttgt cgtaatgcag ggcagcggtc
     1081 gaatgacgat cgatggtgtg gagcaccccg tgcgcgcatt cgatgtcatc gtcattcaat
     1141 ctggtgagga gtggagcatc cgcggcgacg acgtggatcc ggcagtgatt ctcgttctcg
     1201 gcgcgggcgc ttgaagtggc atttgacctc gaaacgctcc ttctcctcga agcgatcatt
     1261 cttcttgcgg cattcataca aggcgcggtg ggattcgcgt tcggaatgac gagtatggga
     1321 ttggccgtgt tcctcatcga cgcgagagtt gcctcgatca tcgtctcacc gctcgccgca
     1381 acgaacgttg cgcttgcact ttggagtgtg cgtcacgcga ttcgccttcg caacgtcatc
     1441 ccgatggtcg ttggaatggt tatcggtcta ccgtttgggc tactcatact cctcgaaggc
     1501 tcgacgacca tcatccggat atccgtagca gcgcttctga tatacgttgg cacgtcgcgt
     1561 ctgctatcga acgggcgcac gcgccgacca ctgcacccgt ggtggggata tgtcgccgga
     1621 cttttcgccg gtatagtcgg tggagcgacg aatatcggtg gccccccgtt gatcgcgtat
     1681 gttgcccatc agccgtggaa gccggtcgtc ttcaaggcct cgctgctgac gtgctttctc
     1741 gtgagttccg gcttgaaaac gatcatgctc ggtatcgagg gctcgctcga ttcgcaattg
     1801 ctcgcgagtg ttgccgtatt gctaccggca gtcgtgatcg gatcacagat cggcatcgca
     1861 tttttcaatt ccgtaaatca gcggctgttt ggacaccttg tctccggtct cattgtc
//
LOCUS       DSCC01000265            1703 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047879, whole genome shotgun sequence.
ACCESSION   DSCC01000265 DSCC01000000
VERSION     DSCC01000265.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047879"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..111)
                     /locus_tag="ENN56_03265"
     CDS             complement(<1..111)
                     /locus_tag="ENN56_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74538.1"
                     /translation="MTPIDVAIFWHMHQPDYREPESGQLALPWVRLHAIKG"
     gene            complement(151..1194)
                     /gene="galT"
                     /locus_tag="ENN56_03270"
     CDS             complement(151..1194)
                     /gene="galT"
                     /locus_tag="ENN56_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_228704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDS74539.1"
                     /translation="MSELRKDPVTNRWVIISTERGKRPSDFAPVQRVDRPKLCPFCPG
                     NEERTPPEVYAVRPSGGAPNSRGWEVRVVPNKYPALRTETEMKREGIGMFDMMSGVGA
                     HEVIIETPHHDERFWDYSESHIETILDTYLQRYRDLRKDFRLRYVLIFKNEGEQAGAS
                     LAHSHSQLIATAVIPKRVREELDGAERYFVYKERCVFCDQIREEARFAERMVHENEHF
                     VAYCPFASRFPFEVTVIPKRHAIDPLGINSAERAALAEMLRTITRKLNEALDSPQYNW
                     IFHVAPANRSASRDPYLDECFHWHIEIIPRLTKIAGFEWGTGFYINPTTPEDAAGYLR
                     EVLGRGSDKESAK"
BASE COUNT          337 a          529 c          458 g          379 t
ORIGIN      
        1 gcccttgatc gcgtgcaggc gcacccacgg caatgcaagt tgtcccgatt ccggttcacg
       61 gtaatcgggc tggtgcatgt gccaaaagat cgcgacgtca atcggagtca tgccgactcc
      121 acgagatgca agccatcggc tcgagcggta tcatttcgcc gactccttgt ccgatccgcg
      181 tccgagcacc tcgcgtaggt atccggcggc gtcctccggt gtcgtcgggt tgatgtaaaa
      241 acccgtaccc cattcgaatc ctgcgatttt cgtcaagcgc gggatgatct ctatgtgcca
      301 gtgaaagcac tcgtcgagat acgggtcgcg actggcggag cgattcgccg gcgcgacgtg
      361 gaatatccag ttgtactgcg gactgtcgag agcctcattc agctttcgcg tgattgtccg
      421 cagcatctcc gccaacgcgg cgcgttccgc cgaattgata ccgagcggat cgatcgcgtg
      481 acgctttggg atgaccgtaa cttcaaaggg gaaacgcgat gcaaatgggc agtacgcgac
      541 gaagtgctcg ttttcatgca ccatgcgctc ggcgaaccga gcctcctcgc gaatctgatc
      601 gcagaagacg caacgctcct tgtacacgaa gtaccgctct gctccgtcga gttcctcgcg
      661 gacacgtttc ggaatcaccg ccgtggcgat gagctgcgag tgcgaatgcg cgagcgacgc
      721 tccggcttgc tcgccttcgt ttttgaagat gaggacatac cgaagtcgaa aatccttccg
      781 cagatcgcgg taccgctgga gataggtatc gagtatcgtc tcgatgtgcg attcgctgta
      841 atcccagaat cgttcgtcat gatgcggcgt ctcaataatc acctcgtgcg cgcccacccc
      901 gctcatcata tcgaacattc cgataccttc gcgcttcatt tcggtttccg tgcgaagcgc
      961 cgggtatttg ttcgggacga cgcgcacttc ccatccacgg ctgttcgggg caccgcccga
     1021 cggacggacc gcgtacactt ccggcggcgt ccgctcctcg ttgcccggac agaatggaca
     1081 cagcttcggt cgatctaccc gctgaactgg agcgaaatct gaggggcgct tcccgcgctc
     1141 tgtcgatatg ataacccacc ggttcgtcac cggatcttta cgcaattccg acatgtattc
     1201 tccgttcagg ctcatcgata cgcgtctgag ccgttaacct gtcttacgcg aaaacgtagc
     1261 cccgtggtat gacgacaaca ccgttttccg ttacgagaaa gcggcggcgg tcagcttcgg
     1321 tatcgaaacc aatcacctct cctgcgggaa tcctgttcga cttatcgacg agtgtgcgac
     1381 ggattcgtgc accacgcccc acctgcacgc cgttcatcaa caccgattcg gatacttccg
     1441 cacctgaatc gatcacgacg tcggtcgata gaaccgaccg ttcgatcttt gcacccacga
     1501 tccttacgcc tttcgcgacg atcgaatcgt taatgaccgg cggatttccg tcgccatccg
     1561 taccgattct cgatggaggc cgctgctcct ggtacgtccg aatcggccac gccggatcgt
     1621 agatatctcc cggtggatcc gctttgagta agtccatcga cgcttcccag tagctatcga
     1681 gcgtaccgat atcacgccaa tac
//
LOCUS       DSCC01000266            2168 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034480, whole genome shotgun sequence.
ACCESSION   DSCC01000266 DSCC01000000
VERSION     DSCC01000266.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034480"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..872)
                     /locus_tag="ENN56_03275"
     CDS             complement(<1..872)
                     /locus_tag="ENN56_03275"
                     /inference="COORDINATES: protein motif:HMM:PF10043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HDS74540.1"
                     /translation="MTSRGNAAGVRLIASVLLAVLALCGSRASAEMSLTDSLRIASSS
                     FRIHATTEWNDLPNYRHERIVPWKLATAIAGVGVYDLAFYQTLKKPWWSGETSRFHVI
                     NDWWGGYALEVDKLGHAFAAQSLTLISAETYRWAGMSRREALFWGGVSSLATMTQIEV
                     LDGFTTAYGFSPADYTANIVGAFWPLAQELWRPLGVVTFKMSYHTARFEQAAAPNVIE
                     DYNRQTYWLAFDVNRMIPEPTRGWWPDWLGVAVGYGVTNAFTYVSHREYNAIRPHRSY
                     LVTRRPAHMMREYYV"
BASE COUNT          451 a          669 c          602 g          446 t
ORIGIN      
        1 acatagtact cccgcatcat gtgcgctgga cgtcgcgtga cgagatagct gcgatgagga
       61 cgaatcgcgt tgtattcgcg atggctgacg tacgtgaacg cgttcgtcac gccgtacccg
      121 accgcgacgc cgagccaatc gggccaccaa cctcgtgtcg gctccggaat catccgattt
      181 acgtcgaatg caagccaata cgtttgtcgg ttgtaatctt caataacatt cggtgcggcg
      241 gcttgctcga aacgagccgt gtggtagctc atcttgaacg tgacgacacc gagcggtctc
      301 cacaattcct gcgcgagtgg ccaaaacgcg ccgacgatgt tcgcggtgta atccgccggg
      361 ctgaatccat acgctgtcgt gaaaccgtcc agtacttcga tctgcgtcat tgtcgcaagg
      421 ctggacacac ctccccaaaa cagcgcttca cgtcgcgaca tgcctgccca tcgataggtc
      481 tccgcggata tcagcgtgag tgattgcgcg gcgaacgcgt gaccgagttt gtccacttcg
      541 agcgcgtatc cgccccacca gtcgttgatc acgtgaaacc gcgaagtctc tccgctccac
      601 catggcttct tcagcgtctg atagaacgcg aggtcgtata cgccgacgcc cgcgattgcc
      661 gttgcgagtt tccacggaac gatgcgttcg tggcggtaat taggcagatc attccactcc
      721 gtagttgcgt gtatacgaaa cgacgacgac gcaattctca gcgaatcggt aagagacatc
      781 tcggcgctcg cacgtgatcc gcataacgcc agtacagcaa gcaacacgct cgcgatgagt
      841 ctcacacccg ccgcattccc acgcgaggtc attggagcac tcccgcgtgt atgagaccga
      901 tctgtgtgct caacgtcacg atatcacgcg gcgacatgcg ctggagcgag atcaccggtg
      961 caccttgtag atgtcgcgta acgatggcga gataccgccg ttcggacccg gaccgctcta
     1021 acacaacgcc gccctgactc tctcgcacct gaacgattac cgcctgctcg aatccatcga
     1081 ttgtcggaaa cagaccgtac gcgcgtgcac tcgacgcggt aatgacactg cccacctgtt
     1141 cgtggagttg aatgacaatc ccgtcatgtg cgagctggtc gaactgcgcc gcactccttg
     1201 gtgaatcgaa gccactccca acgagcagcg tctcgctcgc gcggagtttg gaatcagtct
     1261 gtacgacgtg atcagcgagg tactgatcgg tcgttcgacc agcttgatcg tcgagattcg
     1321 cgggaaacag cgcataatcg actcgcggca cgtcgcgctt gatgcgccgt ccgattgtcc
     1381 agccgatgag cgtgccgatt gcggcaaagg tcaccgtgta gaaaaacgtt cgaactgcgg
     1441 gagacgagcc gccaccggaa ggctgcgagg gttccgtacc tggtctggag gtcggttcgc
     1501 tcgaaccact cgatccatat tggttgtccg catagatatt gacgtaaacg accgaattgc
     1561 cgctcgattc gctcgatccg tagtcaacat cgccgctcgt accaccacct cccgcggacg
     1621 atccaccgct gcttgcactt tcatctccat acgtgctcga tccggtcgcg gtcggatcgc
     1681 cgcctcccgc ggacgactgg ctcgaagcgc cggtgccggt gtcatacgtc tccgatatcg
     1741 tcgattgacc accggatgcg cttgcggtgt tcggagtcgc cgcagcagtc gattcgcccg
     1801 ttgacgattc gtcttcgcca tagtcgtatt ccgactcgcc gattcgtgcg attcgagtgg
     1861 caacggcagg gcgggaagcc ggattcgata tgccgttcga ctgaccgttc gccatatatg
     1921 agagagcgaa accggtccca aggccaatta atccaccggc aattgcgccg tcacgcgcgt
     1981 gtgaaaggtc gaggacgttg acgaggtagc ggatatcacc gacgggaaca gacaaatatc
     2041 ccgaggttgt gttcagctga atggtcttcg caccgcccga tacaaacgcg ccagtaatcg
     2101 aacgcccgtc cgtcgttcca atcatcacgc cgcgcccgcg tccgagcgcc tgccacagat
     2161 cgtaatat
//
LOCUS       DSCC01000267            3779 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016326, whole genome shotgun sequence.
ACCESSION   DSCC01000267 DSCC01000000
VERSION     DSCC01000267.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016326"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(1278..>3779)
                     /locus_tag="ENN56_03280"
     CDS             complement(1278..>3779)
                     /locus_tag="ENN56_03280"
                     /inference="COORDINATES: protein
                     motif:HMM:TIGR03936.1,HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDS74541.1"
                     /translation="VLYHLVNRRDEALAERAYCPWPDMEREMRRADVPMFSLESRMPL
                     GAFDLVGFSLSYELTYTNVLTMLDLAGIPLFARDRTDEHPIVIGGGATMANPEPIADF
                     FDAFLIGDGEEAIDRIIDTVKEHRESGGERHELLESLANIEGMYIPQFYRIEYRDDGT
                     IERFVTLNDSARLPVRRHIIHDLKPEYAPDRPLVPVMEVAQDRLSIEVLRGCTQGCRF
                     CQAGFFYRPVRERSPEEVIEIAQAGIDSGGWDDIALLSLSTADHSRAEELVERVSNAF
                     KDRGVSISLPSLRADRFSLHLCETAGEVKKNGFTFAPETGSERLRRVINKNVSKEEMI
                     QAARVAFEKGWRLIKLYFMIGLPTETMDDVQSIIDLTDEIANVGRAIRSDARVNASVG
                     CFVPKTFTPFQWTAFAGVDTLNERLRYLQTHLTNRAVKLKWHRPEEAWLEAVISRGDR
                     NLSKVIYEAWKRGARFDGWTEWSNTDRWRDAFRTVELDPDQYLMERDPEETLPWDVID
                     IGVSKRYLKLEWKRAHEEQRTQDCRHGDCNACGIPGMPDDNVLTADRERKLLSIEAED
                     RAVNETKRAHTIPGQGFGGGTYRIRYEVDEPFRFVGHSDLMRTFHRACEIANLDVAMS
                     QGYTPRPKAGFGPPVPHGCTSAGEYLDLELNVGVDDLVGVLNGTLPEGIRVLASTRIG
                     EKADAISAVIGAAEYEFTFDADVMSPEDVVTRLTEFDAADKWEATKVNAKGKERVVDL
                     KHAIIAHRVRHMDSRSIVTTIGRLNDPNGNNINPLFVLEHVFGLDEERRTRVVMRRSE
                     LYDRSGRPMSGIAWGGQKPRHLHSRSREFFKFLDT"
BASE COUNT          729 a         1091 c         1119 g          840 t
ORIGIN      
        1 cgttcacggt caacggtggt ttcgcgcatc gtccgcattt tgccggatac agcgaaacgg
       61 tcccgcgtgg acgcgcgcac tcgttcatga tgctcgacct tcagcgccag tattgggccg
      121 atcatatcga ggttgcgccg gagatcgaaa actttccgtt caccgcgtgg aataaatccg
      181 atacccaaac atggtcagag atgacgcttt cgaggttctt tggcgcggat gcgatgttcc
      241 tcgacctcat gccgttcagc aacacacagg aattcgacca gcgcggcatc ggcgaacttc
      301 tcgacgcaag ccgttcggcg ctcgattgga ttggcgaacg cttcgatcgt gcgtttacga
      361 cgcgaggcat cggattaccg tggcgagaag atgcggcgga gcgggtccac acgtcgcacg
      421 gcacggctgt tgatgaactc agtgtacatc ccttcgaccc gggacacttg ctgcttccat
      481 acggcattcc cgttacgtcc gatatcagtc ccgtaaatgc cgtgtttgga cggatggcgt
      541 gggttcacaa taacgcggcg ttagaaacca tgctcgccgg tgggctcatc ctcgacggtg
      601 aagccgcggc gattctgtgt gaacgggggt acgcggagca gattggtatc aattttgtgg
      661 aatggtacga gcgggacagg tcgttgtatg cggtggaggt cgttgtttca ccggaaagcg
      721 gaatctccga ccgtgcgacg ctggatacga acctcatccc gcgcatagca cacgtggagc
      781 ctattcacgg tgcgacggag tggacgtcga tcgcaacacc gacgctggaa cgcgtcggcg
      841 caggcgttac ggtgttccgc aacgcgctgg gcggtcgtgt cgttacgttc gccgcgccga
      901 atccggtatc attgccaccg aactacatcc ggcaaaccat cttgcacagc gcggtacagt
      961 ttgccgggga tgccgcgacg cctatgatgt gggtcacggg aacgccgcac cttatgccga
     1021 ttgagttcgt cgccaaggat ccgaaggaat cccgtcggtc gattgtcgtg tataacggcg
     1081 cgaccgatgc cggtagagcg ctcgttcacg tgcgggatgc gtcgacgccc ccgtcggaag
     1141 cgacggtgct tgcaccgctc tgtgaaccga cgtccattga cgtgtcgtgc gaggcgaagg
     1201 cggatgtgat gacggtcgtc ccttcggtgc gcatcccatc acaggggtat ctcgtgctcg
     1261 agtggcggta acgccgatca ggtatcgagg aacttgaaga actcgcggct tcgactgtgg
     1321 aggtggcgcg gtttttggcc accccacgcg atgccgctca tcgggcgtcc gctccggtcg
     1381 tagagctccg atcgccgcat gacgactcgc gtgcgccgct cttcatcgag tccgaacacg
     1441 tgctcgagta cgaagagcgg gttgatgttg tttccgttag ggtcgttcag ccgaccgatg
     1501 gtggttacaa tcgatcggga gtccatgtgg cgaacgcgat gagcgatgat tgcgtgtttt
     1561 agatcgacga cgcgttcctt gcctttcgca ttgaccttcg tcgcctccca tttatccgcg
     1621 gcgtcgaact cggtgagtcg cgtaacgaca tcttcgggac tcattacatc cgcgtcaaac
     1681 gtgaactcgt attccgccgc gccaataacc gccgaaatcg cgtcggcctt ttcgccgata
     1741 cgcgtcgagg cgagcacgcg gattccttct ggcaacgtgc cgttcagcac accgacgaga
     1801 tcgtcgacac caacgttcaa ttcaagatcg agatactcac cggcgctcgt gcatccatgg
     1861 ggaacgggtg gtccgaagcc ggctttcggg cgtggcgtat acccctgcga catggcaacg
     1921 tcaagattgg cgatttcaca tgcgcgatga aacgtgcgca tgagatcgga gtgtccaacg
     1981 aagcggaatg gctcgtcgac ttcataacgt atccggtacg tgcccccgcc gaatccttgt
     2041 ccgggtatcg tgtgcgctcg tttggtctca ttcaccgctc ggtcttccgc ttctatcgag
     2101 aggagttttc gttcgcgatc cgccgtcaac acgttgtcat cgggcatgcc gggaatgcca
     2161 catgcgttac agtctccgtg ccgacagtcc tgagtccgct gttcctcgtg tgcacgcttc
     2221 cattccagtt tgaggtaccg ttttgaaacg ccgatatcaa tgacgtccca cggtagcgtc
     2281 tcttccgggt cgcgctccat aagatactga tccggatcga gctcgaccgt acgaaaagcg
     2341 tcgcgccatc ggtcggtgtt ggaccattcg gtccagccgt cgaatcgggc gcctcgtttc
     2401 cacgcctcgt atatcacctt gctcaaattg cgatcaccgc gactgattac cgcttcgagc
     2461 cacgcttctt ccgggcgatg ccatttgagt ttcacggcgc ggttcgtcag gtgagtttga
     2521 aggtaccgta gtcgctcgtt gagcgtatca actccggcga acgcggtcca ctgaaatggg
     2581 gtgaaggttt tcggtacgaa gcagccgacc gacgcattta cccgtgcgtc ggaccggatc
     2641 gcgcgtccga cgttcgcgat ttcgtcggtg agatcgataa tactctgcac gtcgtccatg
     2701 gtttcggtcg gcaggccgat catgaaatac agcttgatga gccgccagcc tttttcgaac
     2761 gcgacacgcg ccgcctgtat catttcttct tttgagacgt tcttattgat aacacggcgg
     2821 agtcgctcgg aaccggtttc cggtgcgaag gtgaatccgt tttttttcac ctcgccggcc
     2881 gtctcgcaga ggtgaagcga gaatcggtcc gcgcggaggc ttggcagcga gatactcaca
     2941 ccacggtcct tgaacgcgtt cgagacccgc tccacaagct cttctgcgcg ggagtgatct
     3001 gctgtcgaaa gcgacaacag cgcaatgtca tcccatccac cagagtcgat accggcttga
     3061 gcgatttcga tgacctcttc gggtgatcgc tcgcgaaccg gtcggtaaaa gaaccccgcc
     3121 tgacagaagc ggcatccctg cgtacatccg cggagcactt cgatgctcag tcggtcctgt
     3181 gcgacttcca taacgggcac aagcggccta tcaggtgcgt attcgggctt cagatcgtgg
     3241 attatatgtc gacgaaccgg tagccgcgcc gaatcgttca gggtgacgaa gcgttctatc
     3301 gtgccgtcgt ctcggtactc gatgcggtaa aactgtggga tgtacatacc ttcgatgttc
     3361 gcgagcgatt cgagcagctc atgtcgttct ccacccgact cgcgatgctc tttcaccgta
     3421 tcgatgatcc gatcgatcgc ttcttctccg tcaccgatga gaaacgcatc gaagaagtcg
     3481 gcaatcggtt cgggattcgc catcgtcgcg ccgccgccaa tgacgattgg atgctcgtcg
     3541 gtgcggtctc gcgcgaagag cggaataccg gcaaggtcga gcatggtgag aacgttcgta
     3601 taggtaagct cgtaggaaag cgagaaaccg acgagatcga acgcaccgag tggcatgcgt
     3661 gattcgaggc tgaacattgg gacatccgcg cgccgcattt cgcgctccat gtccggccac
     3721 gggcagtacg cacgctcagc gagtgcctca tcgcgacggt tgacgagatg gtacaaaac
//
LOCUS       DSCC01000268            2181 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034216, whole genome shotgun sequence.
ACCESSION   DSCC01000268 DSCC01000000
VERSION     DSCC01000268.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034216"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(379..1395)
                     /locus_tag="ENN56_03285"
     CDS             complement(379..1395)
                     /locus_tag="ENN56_03285"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74542.1"
                     /translation="MISRERVFAALNHREGDRVPLYIWTFGNPIEGPICEKYGGLREF
                     FDAWDLDMIQSFPGAGMLAERPVSEDMPDISDSAGLPSKLNAYGSVLTLEEALDAPFR
                     DPSSRDIYDPIARDVSYHRDFRGRAIFVQTPGVFESTNGLLGLQGTLEYMATDPDKLG
                     ELFIKIANWAAAYAEYCIDVGADVIHISDDWGRNDAMLFSPATWHKIVGPAEAITAQR
                     VRGRGAYVSLHCDGYFWPVAAELPSMGIQCVHPVQQSAGMDMARFKREFGQKLCIYGG
                     LDVRTTLGTGDLDLIRAEVRERMAQLKPGGGYIFCTSHMVQPGTTLEEVEVALETAHE
                     SAKY"
     gene            complement(1439..1813)
                     /locus_tag="ENN56_03290"
     CDS             complement(1439..1813)
                     /locus_tag="ENN56_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74543.1"
                     /translation="MARFTVIIGIMLIALGVLGYSMTGRESVTALIPAFFGLPLGVLG
                     WIGLQPERVKLTMHIAVVMALIGLIGSVRGIPAFVEMMSGGEVARPGAAVAQTLMALI
                     CGVYVVASIRSFVQARLRRKAG"
     gene            complement(1929..>2181)
                     /locus_tag="ENN56_03295"
     CDS             complement(1929..>2181)
                     /locus_tag="ENN56_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74544.1"
                     /translation="SRKAEIVQNRQMAMYLCKVLTPLTFRAIATEFGGRDHATVVHSC
                     RKFTERVRRDPSLLWEAKLIAKKLGYPNADLDPGSSNGN"
BASE COUNT          470 a          654 c          633 g          424 t
ORIGIN      
        1 acgccgtatg gcgtcgagaa cacgcgcttt cggtaattcg gtaccttccc tcgtttgaaa
       61 ccgaagaagc gctcggtctc ggttcggaag acccggcgga gtgcgtgcgg atcgtcgcgg
      121 cggaagggct cgcggtgttt cgcggtgatg tcgcgctgcc ggtcattcgg acaatctacc
      181 gaggtgcatc ggtagcgggt cggttgcgtg tcgtcgatgc cgtcggcggt atcggcagtg
      241 agaaagcgcg cgcgatgctg cgggagatct cggtgctcga tgcgtccgcg gaggttcgtg
      301 cgcgtgcacg acactatctc agcgaaagcg cgttgggaga accctcggcc gtctgaacga
      361 gcgcgaatcg gcgaaagctc agtacttcgc cgattcgtgg gccgtttcca aagcgacctc
      421 aacttcttcg agtgtcgtgc ccggctggac catgtgcgac gtgcagaaga tgtacccacc
      481 gcccggtttc agttgcgcca tgcgttcgcg gacctcggca cgaatcagat caaggtcgcc
      541 ggtgccgagc gtcgtccgca catccaaccc gccatagata cagagcttct gtccgaactc
      601 ccgcttgaaa cgcgccatgt ccattcccgc cgactgctga acggggtgaa cgcactggat
      661 tcccatggag gggagctctg ccgcgaccgg ccagaaatac ccgtcgcagt ggagcgaaac
      721 gtacgcgccg cgtccgcgta cgcgctgtgc tgtgatcgct tcggccggac ccacgatctt
      781 gtgccaggtc gccgggctga agagcatggc gtcgttgcga ccccaatcat ccgagatatg
      841 aatcacgtcc gcgccgacat cgatgcagta ttccgcatac gccgccgccc agttcgcaat
      901 tttgatgaac aactcaccga gtttatccgg atcggtcgcc atatattcga gcgttccctg
      961 aaggccgagc aatccgttcg tcgattcaaa cacgccggga gtctgtacga aaatcgcacg
     1021 accgcgaaag tcacgatggt acgaaacatc ccgcgcgatg ggatcgtaaa tatcccgact
     1081 ggagggatct cggaacggcg cgtccaacgc ctcttcgagt gtcagcacac ttccatacgc
     1141 attcagttta ctcgggagac cggccgaatc cgatatgtcc ggcatatctt cagatacggg
     1201 tcgttccgca agcattcccg caccgggaaa cgactggatc atgtcgagat cccacgcatc
     1261 gaagaattcg cgcaaacctc catatttctc gcagatcggc ccctcgatgg ggttcccgaa
     1321 cgtccagata tacagcggta cgcggtcacc ctcgcgatgg ttgagcgccg cgaaaacccg
     1381 ttcgcgcgat atcatgcgga atatctcccc ttttcaccac acccgttacg gttctgcctt
     1441 aaccggcttt tcgacgaagg cgcgcctgga cgaacgaacg aatcgacgcc acgacgtata
     1501 cgccgcagat gagcgccatg agcgtctgcg cgacggccgc accgggacgc gcaacctcac
     1561 caccgctcat catctcgacg aacgcaggaa tgccacgcac cgatccgatg agtccaatca
     1621 gcgccatgac gacggcgatg tgcatcgtga gtttgacacg ttccggctgg agtccaatcc
     1681 atccgaggac gccgagtgga agtccgaaaa acgccggtat aagggcagtt acgctctcgc
     1741 gccccgtcat tgaatacccg agaacgccaa gcgcgattaa catgatgccg atgataacgg
     1801 taaaccgagc catctataca gtccttcaac aaaaggagtt acgaagtcga caaaacatca
     1861 gacgggaagg tacaaagaaa tcctgtcgag gaaaccatct gggggatgaa actgttggtc
     1921 tggcggaatc aattcccgtt tgaggatccg ggatcgaggt ccgcgttggg gtatccgagt
     1981 ttcttcgcga tgagcttcgc ttcccagagg agcgaaggat cgcgtcgtac acgctcggtg
     2041 aatttgcggc aggagtgtac gaccgtcgcg tgatcacggc caccgaactc cgtggcaatg
     2101 gcgcggaagg tcagcggcgt aagcaccttg cagagataca tcgccatctg acgattctgg
     2161 acgatctcgg ctttcctgct c
//
LOCUS       DSCC01000269            3936 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1015432, whole genome shotgun sequence.
ACCESSION   DSCC01000269 DSCC01000000
VERSION     DSCC01000269.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1015432"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..509
                     /locus_tag="ENN56_03300"
     CDS             <1..509
                     /locus_tag="ENN56_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS74545.1"
                     /translation="GLSVCRRLKSNADTQDIPIVMLTARGEEADIVRGLEAGADDYIT
                     KPFSPRVLLARMQAVLRRAPKNSTNEDDMITHGRLKIRPGHHEVVIDDVPVELTYTEF
                     AILAYLASRPGWVFTRLQILDAVRGDNYEVTERSVDVHIVSLRRKLGDAGSSVETVRG
                     VGYRFRED"
     gene            513..2291
                     /locus_tag="ENN56_03305"
     CDS             513..2291
                     /locus_tag="ENN56_03305"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF00672.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDS74546.1"
                     /translation="MTHHRLMWQIFPSFLVITFVSLLAVAVFGTRTIRNLYITHLTDK
                     LTTIGRLAEYSTVSHLSLGDIPSAASTVRDIATRSGARITIVEPNGRVVIDSDEDPAT
                     MDNHADRPEIQTAFRGDIGAETRYSYTLATSMLYVALPYYRDGSIAYVARVAVAVADI
                     EDALRGAYVSVFVGWIVIVVIAALVSLYIARRISKPVEEIQRAAEQLAEGNLRARIPI
                     HASSEMVEVSNAMNRAGALLEDRFNTLVRQRNEQEAVLSSMVEGVIAIDTEERIINVN
                     RAAAQLLGVDATKASGRLIQEIVRNTELQQFVQQTLESESPIEADLIYQRVDERFLQA
                     HGTVLRDAQGRAIGALVVLNDVTRLRRLERVRSEFVSNVSHELKTPITSIKGFVETLR
                     EGALDHPEDARRFLDIVSRHTDRLERIVEDLLMLSRLEREAEQGEIEAEPTHVASVVD
                     AAVQLCQVHAEQKGIRVRTQCDENLRARMNAPLVEQAIANLIDNAIKYSDPDSAVDVT
                     AVLTGNEVAISVRDEGTGIEERHLERVFERFYRVDRARSRELGGTGLGLSIVKHIANV
                     HHGRVAVESVLDEGSTFTILLPFTSD"
     gene            2448..3314
                     /locus_tag="ENN56_03310"
     CDS             2448..3314
                     /locus_tag="ENN56_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS74547.1"
                     /translation="MKRLSMMVFSIAVMAATFACSRNSGDVDAEQTYLTIKGSDTMVH
                     LVSTWAEDYMTKHPNTEISVTGGGSGTGIAALINGTTDIAAASRDMKSGEIENARARS
                     IEPVEIAVARDGIAVVVHKENPVTALSTQQLEKIYTGQTTNWRDVGGEDRDILVLSRE
                     SNSGTYVFFQEHVLNKKDYSPRARLMPATSGILQSVANDRGAIGYVGLGYVEGGEESR
                     VKTIGVQKDSEHDAVFPSSETVNSGEYSIARPLFFYTNGEPSGLISQFIEFVLSPEGQ
                     NVVEETGYVKVR"
     gene            3329..>3936
                     /locus_tag="ENN56_03315"
     CDS             3329..>3936
                     /locus_tag="ENN56_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDS74548.1"
                     /translation="MIDKKWKEQGVRVVLILAASTSLVVVALIFIFLFREAAPFAIKP
                     GIGEIFSSRWTPVSFQRESFGIYPLVTGSLLVTAIATIIAIPFGVCGAVYLAEMANNK
                     EREVLKPFIELLAGIPSVVIGFFGLVVLAPIVKQVFGLPSGQTALTGALLLALMAIPT
                     IMSISEDAIRSVPVSYRQASLALGASRLQTIWTVTVPAALPG"
BASE COUNT          919 a         1020 c         1083 g          914 t
ORIGIN      
        1 acggtctgag cgtatgtcga cgcctcaaat ccaacgcgga cacacaagat attcccatcg
       61 tgatgttgac ggcacgtggc gaagaagccg atattgtccg cggacttgaa gccggcgccg
      121 acgattatat cacgaaacca ttcagtcccc gcgtactcct tgcgcgcatg caagccgttc
      181 tccgccgcgc gccaaagaac tctacaaacg aagatgacat gataacccac ggacgattga
      241 agattcgccc gggccatcat gaagtggtaa tcgacgatgt accggttgaa ctgacgtata
      301 ccgaattcgc gatactcgcg taccttgcgt ctcgcccggg ttgggtgttt actcgtctgc
      361 aaatactcga tgcggttcgc ggagataatt acgaagtgac ggaacgctct gtcgatgttc
      421 atatcgtcag tctgcgacgg aagctcggag atgcgggttc gagcgtagaa accgttcgcg
      481 gtgtcggata ccgctttaga gaagactgat ttatgacgca ccaccgattg atgtggcaaa
      541 tcttcccgtc gtttctcgtg attacgtttg tttcgctcct cgcggttgcg gtgtttggaa
      601 cgagaacgat caggaattta tacatcacac acctaaccga caaactaacg acaatcggtc
      661 gcctcgccga gtattcgaca gtttctcatc tttccttggg cgacattcca tccgcagcat
      721 caacggtacg cgacatcgca acccgctccg gcgcccgaat caccattgtt gaaccaaacg
      781 gtcgtgtcgt tatcgactcg gacgaagatc ccgcaacgat ggataatcat gcggaccggc
      841 cggagataca gacggctttc cgtggtgata ttggtgcaga aacccgatac agttacacac
      901 tcgcaacgtc gatgctatac gttgcgcttc cgtactatcg tgacggatcg atcgcatatg
      961 tcgcgcgagt agccgttgcc gtagcagaca tcgaagacgc acttcgcggt gcctatgtaa
     1021 gcgttttcgt gggatggatt gtcatcgttg taattgcggc gcttgtaagc ctgtatattg
     1081 ctcgtcgaat atcgaaaccg gtggaagaaa tacaacgtgc tgcggaacag ctcgcggaag
     1141 gaaatctgcg agcgcgaatc cccattcacg cctcgtcaga aatggtcgag gtgtccaacg
     1201 cgatgaaccg agcaggcgca cttctcgaag accggttcaa tacacttgtc cggcagcgaa
     1261 acgagcagga ggccgtcctt tcaagtatgg tcgaaggtgt catcgcgata gatacggaag
     1321 aacgaatcat caatgtaaac cgtgcggcgg cacagctact cggcgttgat gcgacgaaag
     1381 catcgggacg tctcattcag gagatcgtcc gcaatacgga gttgcagcaa ttcgtgcaac
     1441 agactctcga gagcgagagt cccattgagg cggatctaat ctatcagcga gtcgacgaac
     1501 gctttctgca agcacacgga acagtgttgc gcgacgcaca aggccgtgcg attggtgctc
     1561 tcgtcgtact gaacgacgtt acgcgcttac gtcgtctcga gcgggttcgt agcgaatttg
     1621 tctcgaacgt atctcacgaa ttgaaaacac caatcacgtc gataaaggga ttcgtcgaaa
     1681 cattacgcga aggtgcgctt gaccatcccg aagacgcacg acggtttctc gatattgtca
     1741 gtcgtcacac cgaccggttg gagcgaatag tcgaggatct actcatgctt tcgcgtctcg
     1801 aacgcgaagc tgaacagggc gaaatagagg cggagcccac gcacgttgct tcggtagtag
     1861 atgcggcggt tcagctttgc caggttcatg cggaacagaa aggtatccgc gtgcgtacgc
     1921 agtgtgacga gaatctccga gctcggatga atgcgccgct ggttgaacaa gcgatcgcca
     1981 atcttattga taatgcgatt aaatatagcg atccagacag cgctgtcgat gtaacggcag
     2041 tgcttacggg taatgaggtt gcgatttccg ttcgcgatga gggaaccggt attgaggagc
     2101 gccacctcga acgtgtgttt gagcgtttct atcgggtcga tcgagcgcga agtcgggagc
     2161 tcggcggcac cggtctcggt ttatcgatcg tcaaacatat tgcgaacgtt catcatggcc
     2221 gcgtcgccgt ggagagtgtt ctcgatgaag ggagcacctt cacgattctt ctgccgttta
     2281 ccagtgactg atccgcatcg atcggtgcac tgacgatatc ctcccgtctc tcattctaac
     2341 cgtgccttaa tacttgcgct ctatcttcca tataccttgt tcatcggtgc ggggtaactc
     2401 cgcttcagtg aagcgcacga acgaaaactc tttctggagg cacgaccatg aaacggctta
     2461 gcatgatggt tttttctatc gcagtcatgg cggcaacgtt tgcgtgcagt cgtaacagtg
     2521 gtgatgtcga cgcggaacag acgtatctca cgatcaaagg atcggatacg atggtccatc
     2581 ttgtaagtac gtgggctgaa gattatatga ccaagcatcc gaataccgag atatcagtta
     2641 ccggcggtgg ctccggcaca ggtatcgccg cgctaataaa tggtacgacc gatattgccg
     2701 cggcttcgcg cgatatgaaa tcaggcgaga ttgagaacgc gcgtgcacgt agcatcgaac
     2761 ctgtcgaaat cgctgttgcc cgtgacggaa ttgcggtcgt agtacacaaa gagaatccag
     2821 tgaccgctct cagtacccag caactcgaga aaatctacac cgggcaaacc acgaattggc
     2881 gtgatgtcgg tggagaggat cgggatatac tcgttctctc tcgcgaaagt aattcgggga
     2941 cgtacgtgtt ctttcaagag cacgtcctga acaagaagga ctactcgccg cgcgcgcgat
     3001 tgatgcctgc tacgtccgga atacttcaat cggtcgccaa cgaccgtggg gctatcggat
     3061 acgttggtct tgggtacgtc gaaggcggtg aggagagtcg cgtcaaaaca ataggagtgc
     3121 aaaaagactc agaacacgat gccgtcttcc cgtcgtctga gacggtcaac agcggtgaat
     3181 actcaatcgc gcgtccgtta ttcttctaca caaatggcga gccgagcggc ctaatatcgc
     3241 agttcatcga gttcgtgctc agcccggaag gccaaaacgt ggtcgaagag accggatacg
     3301 taaaggttcg ataggcgggt atcgctcgat gatcgataag aaatggaaag agcagggcgt
     3361 ccgcgtcgtt ctcatacttg cggcttccac aagcctcgtg gtcgttgcat tgatttttat
     3421 ctttctcttc cgcgaagccg cgccgtttgc cataaaaccg gggatcggtg agatcttctc
     3481 ttcgcgttgg actcccgtgt cattccaacg cgagtcgttt ggaatctatc cgcttgttac
     3541 gggatcgctc cttgtaacgg cgattgcgac gattattgcg attccattcg gagtttgtgg
     3601 cgcagtgtac ctggcagaaa tggcgaacaa taaggaacgt gaggtactta agccattcat
     3661 cgagttgctc gccggaatcc catccgtcgt gatcggcttt ttcggattgg tcgtactcgc
     3721 cccgatcgtc aagcaggtgt tcggactgcc ctcggggcaa acagcgttga ccggtgcgct
     3781 tttacttgct ctcatggcaa taccaacgat catgtcgata tcggaggacg cgattcgcag
     3841 cgttcccgtt tcctatcgac aagcgtcact tgctctcggt gcaagtcgtc ttcaaacgat
     3901 ctggacggtt accgtgccgg ctgcactccc tggtat
//
LOCUS       DSCC01000270            2011 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038174, whole genome shotgun sequence.
ACCESSION   DSCC01000270 DSCC01000000
VERSION     DSCC01000270.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038174"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..498)
                     /locus_tag="ENN56_03320"
     CDS             complement(<1..498)
                     /locus_tag="ENN56_03320"
                     /inference="COORDINATES: protein motif:HMM:PF01855.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HDS74549.1"
                     /translation="MSAFGDDYGCGKGTLMADAVLTGAEAMARAAELAGVRIAPMFPI
                     TPQTAVIETLAQNGVVETLRANSEYNVMAMAAGVAWAGQRVFTVTSSQGLVMMSEMMW
                     EVAGNRLPVVMGVFGRALKGPGWNLGTQQNDSLMMRDTGWLMFYAETAQELLDFVLIA
                     YRVAEE"
     gene            complement(453..>2011)
                     /locus_tag="ENN56_03325"
     CDS             complement(453..>2011)
                     /locus_tag="ENN56_03325"
                     /inference="COORDINATES: protein
                     motif:HMM:PF14691.4,HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS74550.1"
                     /translation="QEGEFSGAHALFLEESPYPAVTGHVCYHPCETWCNRKDHDEPIS
                     INALERAMAEYGDIATDCVSVRYANQPVAVVGSGPAGTTVAYHLARLGYPVTVFERDT
                     QLGGALRYGIPRYRLPDSVLDAAFDRLRALGVTLRSGIQIGVDVPWEQLEREYAAAFL
                     GIGKPRGRTLTVSGIEESGAQSGIEFLRAVNTDALTELDGDVAVIGGGDVAIDCVRSA
                     IRLGADAVRLYCLESRDEMPAHPDEVAEAAAEGMYLHDGWTVTSVERDDSGRLRLDLR
                     DVTSFSPPQPPVLGSRTANVTVDHLIYAIGQEHDADWLPSDLWRDDRIGVDTVGRTLR
                     PMVFGGGDIAGTYNVVQAIGAGKRAALGIDCALRGWDPDVYAQRVSVGAKGAISMRDY
                     SKLRGTLDNGSRQTRDDIVLIDRINLDYFPERPRVKRPELAIRERRGFVEVNGTLTRE
                     QARQEARRCFNCSECTVCGNCFIYCPDSAVLQLEDGFFTIDDDHCKGCGVCVQECPRS
                     AMTMVVERER"
BASE COUNT          413 a          639 c          561 g          398 t
ORIGIN      
        1 ctcttcagcg acgcgatacg cgatcagcac gaagtcaagg agctcctgcg ccgtttccgc
       61 gtagaacatg agccatccgg tgtcgcgcat catgaggcta tcgttctgct gcgttccgag
      121 gttccacccc gggcccttca acgcgcgacc gaatacaccc atgacaacag gcaaacggtt
      181 gccggcgacc tcccacatca tctcggacat catcacgaga ccttgtgacg aggtgacggt
      241 aaacaccctt tgacccgccc acgcgacacc tgccgccatc gccatcacgt tgtactcgga
      301 gttcgcacga agtgtctcga cgacaccgtt ctgggcgagg gtttcgatca ctgcagtttg
      361 cggtgtgatc gggaacatcg gagcgatacg aacgccggcg agttctgcgg cacgcgccat
      421 ggcttcggca ccggtaagga ctgcgtcggc catcagcgtt ccctttccac aaccatagtc
      481 atcgccgaac gcggacattc ctgcacgcac acgccgcacc ctttacaatg gtcgtcgtcg
      541 atggtgaaaa acccatcttc gagctgaagc actgcggaat cggggcagta gatgaagcaa
      601 ttgccgcaaa ccgtacactc ggagcagttg aagcagcggc gcgcctcttg acgcgcctgt
      661 tcacgagtga gagtaccatt cacctcgaca aacccgcgac gctcgcgaat agccagttcg
      721 gggcgtttca cccgtggtcg ttccgggaaa tagtcgaggt tgatgcgatc gatgagaacg
      781 atatcatcgc gggtctggcg tgatccgttg tcgagcgttc cgcgcagttt gctatagtcg
      841 cgcatggaaa tcgcgccttt tgcaccgacg gatacgcgtt gtgcgtagac gtccggatcc
      901 catccacgta acgcacagtc gataccgagc gccgcccgct tacccgcgcc aattgcctgc
      961 acgacgttgt aggtacccgc aatgtctccg ccgccgaata ccatcggtcg caaagtccga
     1021 ccgacagtat cgacaccaat tcggtcatca cgccagaggt cgcttggtag ccagtcggcg
     1081 tcgtgttcct gcccgatggc atagatgagg tgatcgacgg ttacattcgc ggttcgagag
     1141 ccaagaacag gcggctgtgg cgggctgaaa ctcgttacgt ctcggagatc gaggcgcaac
     1201 cgaccactat cgtcacgctc tactgatgtc accgtccagc cgtcgtgcag atacatgcct
     1261 tcagccgctg cttccgcgac ttcgtccgga tgagcgggca tttcgtcgcg cgattcaaga
     1321 cagtagagac gcaccgcgtc ggcaccgagc cgtattgccg aacgcacgca atcgatcgct
     1381 acgtcgccgc cgccgatcac cgcaacgtcg ccatcaagct ccgtcagtgc atcggtattc
     1441 acggcgcgaa ggaattcgat tccgctctgc gctccagatt cctcaatgcc cgaaaccgtg
     1501 agcgtacgac cgcgcggctt gccgattccg agaaacgccg cggcgtattc gcgttcgagt
     1561 tgctcccacg gaacgtctac accgatctga ataccactac ggagcgtcac gccgagtgcg
     1621 cgcagtcgat caaacgcggc atcgagaaca ctgtcgggga gacgatagcg cgggattccg
     1681 tatcgtaatg cgccgccaag ctgcgtatct cgctcgaata ctgtcaccgg gtatccaagc
     1741 cgcgccagat gatacgcgac cgttgttccg gctggtccac ttcctacgac cgcaacgggc
     1801 tggttggcat accgcacaga cacgcaatcg gtcgcaatgt cgccgtattc cgccattgcc
     1861 cgttcgagcg cattgattga gatcggttcg tcgtggtcct tgcggttaca ccacgtttcg
     1921 cacgggtgat aacaaacgtg gccggttacc gccggatacg gactttcttc gaggaacagc
     1981 gcatgcgccc cggagaattc gccctcttgc a
//
LOCUS       DSCC01000271            3916 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1015536, whole genome shotgun sequence.
ACCESSION   DSCC01000271 DSCC01000000
VERSION     DSCC01000271.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1015536"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..994
                     /gene="aroB"
                     /locus_tag="ENN56_03330"
     CDS             <1..994
                     /gene="aroB"
                     /locus_tag="ENN56_03330"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:PF01761.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDS74551.1"
                     /translation="GKKVALVTDDSVGPLYTHRLIHSLETAGYTVLSVTLPAGEKHKQ
                     LGTVERIIDACLEVRFDRNSAIIALGGGVVGDMAGFAAAVLLRGIRFVQVPTTIVAQV
                     DASVGGKTGVDHLRGKNLIGAFHQPSLVYIDVETLSTLPERERIAGLAEVLKHAIIRD
                     AELFRLLESSVDRFADFDATPEEWVALIERNCAIKADIVGQDERESGIRALLNYGHTT
                     GHAIETLGDYDRYRHGEAVLLGMLIAGEIASGRNLLTEPDHKRHNALINRILRVPIPT
                     DYSSSDVWECMLSDKKIRDGVLAFILPTRIGDVTIVRDVKRAEFEAAWDRAITQ"
     gene            complement(1009..1938)
                     /gene="rbsK"
                     /locus_tag="ENN56_03335"
     CDS             complement(1009..1938)
                     /gene="rbsK"
                     /locus_tag="ENN56_03335"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDS74552.1"
                     /translation="MKRPTIVVVGSVNTDLTVQASRVARPGETLLGRDFSVSHGGKGA
                     NQAVAAARLGASVTFVGCVGCDTFGDNAVAALAAEGIDTRFIRRDVDCPSGVALIVVG
                     DDGENSIIVAPGSNATLSTHDVEAAADVFRIADVVLTQLEVPIVTVEKTLCVGRSNGA
                     ITILNPAPAQRLPQSVFDLTDWLTPNETEAALLTGVENENPGEFASVGNRLIESGVGN
                     VVVTRGSAGATYVSTQEMSEVESFHVDAIDTVGAGDAFNGALAVALGSGFTVPEAMRF
                     ASAAAAISVTRRGAQASMPMIDEVERLMRNGTA"
     gene            2089..2727
                     /locus_tag="ENN56_03340"
     CDS             2089..2727
                     /locus_tag="ENN56_03340"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDS74553.1"
                     /translation="MAKRKTVVIGVLASGGGTNMQAIIDRIESGSLDARIAVVVGNNS
                     GAGALDRARRHGIPAFHLSRLTHPSPDELDRVMVNILRAHGVQWLVLAGYMRQVGRKT
                     LTEYRDRILNIHPALLPKYGGEGMYGIHVHEAVIDAGETETGVTVHLVTEKYDTGPII
                     AQRCVAVRNDDTPGSLQKRVLVEEHRIYADVIAAIADGRLTVVDGHPSIRVE"
     gene            2862..3752
                     /locus_tag="ENN56_03345"
     CDS             2862..3752
                     /locus_tag="ENN56_03345"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDS74554.1"
                     /translation="MTQAVRETNFPDVPLFARGKVRDVYDLGNNLLIVATDRVSAFDV
                     VMPTPIPGRGIILTQMSLFWFRLMDDIVSNHVVTADVSQFPNRLQKYADVLEGRSMIV
                     RKANRIDIECVVRGYLAGSGWKDYKKTGTVCGHELPPGLVESSRLPEPIFTPATKAES
                     GHDENISVDHAAEIIGKEVIDELERLSIALYERGRSYADSRGIIIADTKFEFGWIDNR
                     LSLIDEVLSPDSSRFWPKDSYEAGRSQDSFDKQYVRDYLETLDWNKTAPGPELPDEIV
                     ERTLAKYREAYDLLVTSRDH"
     gene            3768..>3916
                     /locus_tag="ENN56_03350"
     CDS             3768..>3916
                     /locus_tag="ENN56_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HDS74555.1"
                     /translation="MVIKRALISVSNKDNVAEFARALVEELGVEVLSTGGTARHLRES
                     EIPVT"
BASE COUNT          903 a         1088 c         1062 g          863 t
ORIGIN      
        1 ggggaaaaag gtggcactgg ttaccgatga ctcagtcggt ccattgtata cgcatcgcct
       61 aatccattcg ttggaaacgg cgggttacac agttttgtct gtcactctac ccgcaggtga
      121 aaagcataaa caactgggca ccgtcgaacg tattattgac gcgtgtctcg aagtgcgttt
      181 tgacaggaat agcgctatca tcgcgctcgg tggcggtgtc gtcggtgata tggcgggctt
      241 cgctgccgcc gttttgttac gcggcatacg gttcgttcaa gtgccgacga cgatcgtcgc
      301 ccaagtggat gcgagtgtcg ggggaaaaac cggcgtcgat catcttcgcg ggaagaacct
      361 gatcggcgcg tttcaccaac cgtcgctcgt ctatattgac gtggaaacgc tctccacact
      421 tccggaacgg gaacgaatcg ccggtcttgc cgaggttctc aagcacgcaa tcatccgcga
      481 cgcagaactg ttccgtctcc tcgaatcctc tgtcgatcga tttgcggatt tcgacgctac
      541 acctgaggaa tgggtcgcac tcattgagcg taactgcgca atcaaagcag atatcgttgg
      601 acaagacgaa cgcgagtctg gaatccgcgc acttctcaat tatggtcata ccacaggtca
      661 tgcgatcgag actcttggag attacgaccg ataccgccac ggagaagcgg tacttctggg
      721 gatgctcatc gccggggaga ttgcatccgg tcggaatctt ttgaccgaac ccgaccacaa
      781 acgtcataac gcactgatca atcgcatact ccgcgttcca attccgaccg actattcatc
      841 gagtgacgtg tgggagtgta tgttgtctga caaaaagatc cgcgacggtg tactcgcatt
      901 catacttcct actcgtatcg gcgacgtaac catcgttcgc gatgtgaaac gagccgaatt
      961 cgaggctgcg tgggatcgcg cgattactca atgaacgtaa ctcccgtgtc aggccgtacc
     1021 atttctcatc aggcgttcga cctcatcgat catcggcatc gatgcctgtg caccacgtcg
     1081 cgtcacactg atcgctgcgg cggcggacgc aaaccgcatc gcttcgggaa ccgtaaatcc
     1141 gctacctaaa gcgacggcaa gagcgccgtt gaatgcatcg ccggccccga ctgtgtctat
     1201 cgcatcgacg tgaaacgact cgacctcaga catttcttgc gtcgatacgt aggttgcccc
     1261 agcgcttccc cgcgtaacaa caacattccc tacgccagat tcgatcaagc gattccccac
     1321 tgacgcgaat tcaccgggat tctcgttttc cacaccggta agtaacgcgg cttcagtctc
     1381 attcggggta agccagtctg tgagatcgaa tacgctctgc ggcaaacgct gcgccggtgc
     1441 gggattgagg atcgtaatcg cgccgttcga acgtccgaca catagtgttt tctcgacagt
     1501 cacgatagga acttcaagtt gcgtaagtac aacgtccgcg atccgaaaca catcggctgc
     1561 cgcttcaacg tcatgcgtcg atagagtggc attcgaaccg ggagcgacaa tgattgagtt
     1621 ttctccatca tcccctacga cgatcagtgc tacgcccgag gggcaatcaa cgtcacgccg
     1681 aatgaaacgc gtatcaatac cttcggcagc caaggcagcg acggcgttgt cgccgaaggt
     1741 atcacacccg acacagccga caaacgtaac cgacgctccg agtcgagccg cagctactgc
     1801 ctgattcgca cccttaccgc cgtgtgagac ggaaaaatct cgaccgagta acgtttcacc
     1861 cggccgagcg acacgagacg cctgcacggt tagatcggta ttgacgctcc cgactacgac
     1921 gatcgtcgga cgtttcatca ttcttccctt ctgaacgttt ctgcgcaaat tgtcctttcg
     1981 aaagttgttc cgattaacgt cggtgtcaac ttggtatact acgtcgtcaa agcctatctt
     2041 tcagtgtcgg cggatgttcg ttacgggaat cgagcgtacg gaaacggtat ggcgaaacgt
     2101 aaaacggttg tgatcggagt cctcgcctcg ggcggcggca cgaatatgca agcgatcatc
     2161 gatcggattg aatccgggtc gctcgacgca cggatcgccg ttgtcgtagg gaacaactcc
     2221 ggtgccggag cgctcgatcg ggcacgccgt catggcattc cggcttttca cctgagccga
     2281 ctcactcacc cgtcgcccga cgaactcgat cgggttatgg tgaacatatt gcgtgcacac
     2341 ggcgtccaat ggctcgttct cgcaggatac atgcgacagg tcggtcggaa aacacttacg
     2401 gagtaccgcg atagaatcct gaacatccat cctgcgcttc ttccgaaata cggcggtgaa
     2461 ggcatgtacg ggattcacgt tcacgaagcc gttatcgatg caggtgaaac agagactggc
     2521 gtaacggtgc atctggttac ggagaaatac gacaccggac caattatcgc acaacgttgc
     2581 gtggcagtgc gaaacgacga tacaccggga agtcttcaga aacgtgttct tgtggaagaa
     2641 caccgcatat acgccgatgt aatcgccgcg atcgcagacg gtcgtctcac cgttgtcgac
     2701 gggcatccat ccattcgcgt cgagtgaatc acgtcaagtc gccgaattga atatccgttg
     2761 tcagacgttt ccgcgttgca tcgcacccct tgccgatggt acttttctcg tcggtggaaa
     2821 cggccgagcg gaacttcctg tgaaccacgt gagagaaaac catgacacaa gcagttcgtg
     2881 aaacgaattt ccccgacgta ccgttgttcg cgcgcggtaa agtgcgtgat gtgtacgacc
     2941 tcggcaacaa cttgctcatc gtggcgactg accgcgtaag cgcattcgat gtcgtcatgc
     3001 cgactcctat tcccggtcgt ggcatcatac ttactcagat gtcgttgttc tggtttcggt
     3061 tgatggacga catcgtatcg aatcacgtcg tgactgcgga tgttagtcag tttccaaatc
     3121 gcctccagaa atacgccgac gtgctcgaag gccgttcgat gatcgtgcgt aaagcaaatc
     3181 gtatcgacat cgagtgcgtt gtccgcggat acctcgcggg ttccggctgg aaggactata
     3241 aaaaaaccgg aacggtatgc ggacatgaac ttccgcccgg actcgttgag agcagcagac
     3301 tcccggagcc gattttcact cccgcgacaa aagccgagag cgggcatgat gagaacatca
     3361 gtgtcgacca cgccgccgaa atcattggca aagaggttat tgacgagtta gagcgtttga
     3421 gtatcgcgct gtacgagcga ggacgctcgt atgccgattc gcgcggaatc atcatagcag
     3481 atacgaagtt cgagttcggt tggatcgaca atcggctttc gctgatcgac gaggtgctct
     3541 cgccggattc gtcgcgcttc tggccgaaag attcgtatga agccggacgg tcacaggata
     3601 gtttcgacaa acagtacgtg cgcgactacc ttgagactct cgattggaat aagaccgcac
     3661 ccggccccga attgcccgat gagatcgttg aacgcacgct cgcgaaatac cgggaagcgt
     3721 atgatctcct cgtaacgtcg cgcgaccatt agaagggatt tgatttcgtg gtaatcaagc
     3781 gtgcgctcat cagcgtgtcg aacaaagaca atgttgcgga gttcgcccgt gcactcgtcg
     3841 aggaactcgg cgtggaagtt ctttcaacag gcggcacggc ccgacacctg cgcgaatcag
     3901 agattcccgt tacgga
//
LOCUS       DSCC01000272            1935 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040277, whole genome shotgun sequence.
ACCESSION   DSCC01000272 DSCC01000000
VERSION     DSCC01000272.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040277"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..865)
                     /locus_tag="ENN56_03355"
     CDS             complement(<1..865)
                     /locus_tag="ENN56_03355"
                     /inference="COORDINATES: protein motif:HMM:PF00291.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDS74556.1"
                     /translation="MLTRPTIDDVRAAVERIRQHVYVTPLEHSAHFSRLLGNRIYHKL
                     ENLQMTGSFKVRGALNAILSLTDEQRASGVIAASAGNHAQGVAYGAEITNTRAVIVMP
                     MATPLVKLEAVKRHGAEVVRHGENYDEACARAYEIAEERGLCMVHAFDNPTVIAGQGT
                     IALEIIEQIPDLDAIVVPIGGGGLISGIGLVVKELAPRVQVIGVQAAGSAAMARSLAA
                     GDIRTVERPVTIADGIRVGRPSDLTTGICRDVVDQVVTVDDDEIANAVLMMVETDKSV
                     VEGAGATPLAAL"
     gene            1034..1552
                     /gene="pyrE"
                     /locus_tag="ENN56_03360"
     CDS             1034..1552
                     /gene="pyrE"
                     /locus_tag="ENN56_03360"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDS74557.1"
                     /translation="MERAKLAKAIKDAAYLEGDFILSSGQRSKYYLDKYLFSTDPTLL
                     KEIAKAFAAILPADYDVLAGLELGAVPLVTAVALEVGKPYVLVRKAAKEYGTSKLFEG
                     RLEAGQRIVLIEDVLTTASQAIDAGKRLRAFGADVVKVIYVIDREQGAAKNLKNAGFV
                     AQALFTKTDLGI"
     gene            complement(1572..1889)
                     /locus_tag="ENN56_03365"
     CDS             complement(1572..1889)
                     /locus_tag="ENN56_03365"
                     /inference="COORDINATES: protein motif:HMM:PF06803.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDS74558.1"
                     /translation="MGKRVFTDGRIPTWNKVVLALLIGIIVSPIDLIPSVIPVFGQID
                     DIAIGLLILDYLFPGIPYQIMIATYRGDPENLLTWQTWARRLTRIIPRRVHRGVFRKP
                     RSR"
BASE COUNT          433 a          579 c          519 g          404 t
ORIGIN      
        1 caagcgcggc gagcggcgtc gcacccgcgc cttcaacgac cgatttgtcg gtctcgacca
       61 tcataagcac tgcgttcgcg atctcgtcat cgtcaacggt gacgacttga tctacaacgt
      121 ctcgacatat acctgtcgtc agatcgctcg gacgcccgac ccgtatgccg tctgcgatgg
      181 ttaccggccg ctccaccgtc cggatgtccc ctgctgcaag cgaacgcgcc atcgccgccg
      241 aacccgctgc ttggacaccg ataacctgca cgcgtggcgc gagctctttc acgacaagac
      301 cgataccgct gatcagtcca ccaccgccaa tcggtacaac aatcgcgtcg agatccggta
      361 tttgctcgat gatctcgagc gcaattgtcc cttggcccgc gatgactgtc ggattgtcga
      421 acgcgtggac catgcacaat ccgcgttcct cagcaatctc gtacgcgcgt gcgcatgctt
      481 catcgtagtt ctcgccgtga cggaccacct cagcgccatg ccgtttcacc gcttcgagct
      541 tgaccagtgg agtggccatc ggcatgacaa tcacggcgcg cgtgttggtg atttccgcgc
      601 cgtacgcgac accctgtgca tggttcccgg cggacgcggc aatcacacca gacgctcgct
      661 gctcatccgt caatgaaagt atggcattca acgcaccacg cactttgaac gaaccggtca
      721 tctggagatt ttccagcttg tggtatatcc gattaccaag caagcgggag aagtgtgcgg
      781 agtgttccag tggtgttacg tacacgtgct gtcgtatccg ttcaaccgcg gcacgaacgt
      841 catcaatcgt tggacgagtc agcataccgg tttcctaaga ttcctttgaa ggaaatgtcg
      901 tagcacagaa ctaacatcgc ggttcgcaat cggggcaaca gccgcctacc ggggagtgtg
      961 aataccggtg ccgaatttgt acctttttcg gtccgcaatt ggcgcagaca agaaagatac
     1021 gggagagcac acgatggaac gcgctaaact tgccaaagca ataaaagacg cagcgtatct
     1081 cgaaggagat ttcatcctca gctctggcca acggagcaag tattacctcg acaagtatct
     1141 cttttcaacc gacccaacgc tcctcaagga aatcgcgaag gcgtttgccg cgattcttcc
     1201 ggccgattac gacgttctcg ccggcctcga gctcggtgcc gtgccgctcg ttaccgcagt
     1261 cgcactcgaa gtaggtaagc catacgttct cgtgcggaaa gcagccaagg aatacggaac
     1321 gagtaaactc tttgaaggac gtctggaggc gggccaacgc atcgttctca tcgaggacgt
     1381 tctcacgact gcttcacagg ctatcgacgc gggaaaacgg ctgcgcgcat tcggtgcaga
     1441 tgtcgtaaag gtcatctacg tgatcgaccg cgaacaaggt gcggcgaaaa atctgaaaaa
     1501 cgccgggttc gtcgcgcaag cgctgtttac gaaaaccgac ctcggtatct gaccgacgcg
     1561 gtaccccgcg ttcatcgcga tcgcggtttc cggaacactc cacgatggac acgacgcggg
     1621 attatcctcg tcaatcggcg tgcccacgtt tgccacgtga ggagattctc cgggtcgccg
     1681 cgatatgtcg cgatcatgat ttggtacggt attccgggaa acaggtaatc gagaatcagc
     1741 aatccgatcg cgatatcgtc aatctgcccg aataccggaa tcacgctggg aatcagatcg
     1801 attggcgaga cgattatgcc gataaggagc gcaagcacga ctttgttcca cgtcggtatg
     1861 cgcccatcgg tgaaaacccg tttcccgatc gctactcagc cagtttccgg aagtcttcca
     1921 cgcgcccgaa cgtga
//
LOCUS       DSCC01000273            2011 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038172, whole genome shotgun sequence.
ACCESSION   DSCC01000273 DSCC01000000
VERSION     DSCC01000273.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038172"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1110
                     /locus_tag="ENN56_03370"
     CDS             <1..1110
                     /locus_tag="ENN56_03370"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02518.24,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS74559.1"
                     /translation="LEAMFANLPVGVLQIDADRTVRAANPAMAEMIGLREDEIVGRRC
                     FQLVGDESHFCDHCLFNRTDVAITRHTRTLHRGTRNERILDTTIVRVPGTNGDDPTVL
                     ELAHDVTDERRLEAQLIQSSKLASIGQLASGLAHEINNPLGTILLRTDSLLADVSEQG
                     LPDDIREDVEAIQRQASRVSRITEELLTFSRCTNVERGPVDVNAVIESAMVIVGLRAE
                     KHVVTVERATVEDVSPVYGNTTQLEQVVVNLLANAIDASRPDSVVTVGARTVREEMNC
                     PVEIWVEDSGSGIAEDELEHIFDPFYSTKDVGEGTGLGLSISYGIVTAHDGTITVSSR
                     YGEGTVVRVRLARLSDTELEVQSRKDTTAERASTA"
     gene            1107..>2011
                     /locus_tag="ENN56_03375"
     CDS             1107..>2011
                     /locus_tag="ENN56_03375"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS74560.1"
                     /translation="MTDVDTQSEDRHTLLVVDDDTDLLASYQKVLRRAGYGVLLAESG
                     TEAFDLMRRHNPSIVLTDIRMPRMDGVTLLREITTNFPDTIVIIMTAFGTVARAVETM
                     KEGAFDFLTKPFSADQLLEVVRRAEYRANVIRRNRDLHEDLGGLCDSDIIVGQSPAMR
                     ALFVHIRTAAQSNGSVLVLGESGTGKELVARAVHSNSSRKAGPFVALNCGALPDHLVE
                     SELFGHEKGAFTGAHESRPGLLTTANNGVFFLDEIGDLPLHLQSKLLRALEERRVRRV
                     GGRTETEFDVRVISATNRNIDEMVN"
BASE COUNT          430 a          550 c          630 g          401 t
ORIGIN      
        1 ctcgaggcga tgttcgcgaa tctacctgtg ggcgtgttgc agatcgacgc ggacagaacc
       61 gttcgtgcgg caaatccggc gatggcggag atgattgggc ttcgcgagga cgagatcgtc
      121 gggcgacgtt gcttccaact cgtcggagat gaatcacatt tctgcgatca ctgtctgttc
      181 aaccgcactg atgtagcgat tactcgacac acccgcacgc tccatcgtgg tacacggaac
      241 gagcggatcc tcgacacaac gattgtgcgt gtgccgggca ccaacggcga tgatccaacc
      301 gtgttggagc ttgcccatga cgtcaccgat gagcggcggc tcgaagcgca actgatccaa
      361 tcgagcaaac tcgcttcgat tgggcaactc gcgagcggac tggcgcacga aatcaacaat
      421 ccgctcggaa cgattctgtt gcgaaccgat agcctccttg ccgacgtgag cgagcagggt
      481 cttcccgatg acattcggga ggacgttgag gcgattcagc ggcaggcgag tcgggtgagt
      541 cggattaccg aagagctgct gacgttttcg cgctgcacga atgtcgagcg cggaccggtg
      601 gacgtcaacg cggtgatcga aagcgccatg gtgattgttg ggcttcgcgc ggaaaaacat
      661 gtcgtcaccg tcgagcgcgc gacggtggaa gatgtctcac cggtgtacgg caacacgacg
      721 cagctcgaac aagtcgtcgt gaatctcctc gcgaacgcga tcgatgcgtc gcgtcccgat
      781 tcggtggtaa cggtaggtgc gcggacggta cgagaagaaa tgaactgtcc cgtggagatc
      841 tgggtagagg attccggttc gggtattgcc gaagacgaac tcgaacatat cttcgatccg
      901 ttctacagta cgaaggacgt cggcgaagga accggactgg gcctaagtat cagttacggg
      961 atcgtcaccg cgcacgacgg aacgattacc gtgtcgagtc gatatggaga gggaaccgtt
     1021 gtgcgtgtac gcctggcgcg tctcagcgat acggagctcg aagtgcaatc ccgcaaagat
     1081 acgaccgcag aaagggcgtc tactgcatga ccgacgtcga tacgcaatct gaggaccggc
     1141 acacgcttct tgttgtcgac gacgataccg atctgcttgc aagttatcag aaggtgctcc
     1201 gacgcgcggg atatggtgtg ctgctcgccg aaagcggcac ggaagccttc gatctgatgc
     1261 gtcggcacaa tccgtcgatt gtcctgaccg acattcgcat gccgcgcatg gacggcgtga
     1321 ccttgctccg cgaaatcacg acgaactttc ccgatacgat cgtaatcatc atgacggcgt
     1381 ttggtaccgt cgcgcgcgct gtcgagacga tgaaagaagg cgcgttcgat ttcctgacaa
     1441 agccgttttc cgccgatcaa ctgctcgaag tggttcgtcg ggctgaatac cgggcaaacg
     1501 taatccgccg caatcgtgat ctgcacgaag atcttggcgg actctgcgac tcggatatca
     1561 tcgtcggtca atcgccggcg atgcgcgcac tgttcgtcca catccgcacg gcggcacaat
     1621 cgaacggtag cgtgctggtt cttggggaaa gtggcaccgg caaggagctc gtcgcccggg
     1681 cggtgcactc gaacagctcg cgaaaggcgg ggccattcgt cgcgctaaat tgcggcgcgc
     1741 tccccgatca tcttgttgag tctgaactct ttggacatga gaaaggcgca ttcaccggtg
     1801 cgcacgagtc tcggcccggg ttgctcacga cggcgaataa cggtgtgttt ttcctcgacg
     1861 agatcgggga tctcccgctg catcttcaat cgaagctcct ccgtgcgctt gaggagcgac
     1921 gcgtgcggcg cgtcggcgga cggaccgaaa cggagttcga cgttcgggtg atcagtgcga
     1981 cgaatcggaa tatcgatgag atggtaaacg a
//
LOCUS       DSCC01000274            2866 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023766, whole genome shotgun sequence.
ACCESSION   DSCC01000274 DSCC01000000
VERSION     DSCC01000274.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023766"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            500..1276
                     /gene="bamD"
                     /locus_tag="ENN56_03380"
     CDS             500..1276
                     /gene="bamD"
                     /locus_tag="ENN56_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDS74561.1"
                     /translation="MIRYRTILIGFATILVAVTLVANTGCARKARRLLTCEEYYDLGM
                     RHLEGKKWLKAQEMFEAITYNYPGCDLVDDAQYMLGETYFRQGLYIEAQFEFRRVVED
                     FRLSDRMEDAQYHMALAAHKQSHPAALDQTPTEEAIFRFQQYLDDFPNGKYADEARGY
                     IREGRKKLAQKDYTAGRFYHRLDYEEAALIYLNHVINEYPDSEEWVERARYLKSVILV
                     ERDQSQEALVLLRTINQDHIKPKLREDIQRLITRIQNPER"
     gene            1273..1914
                     /gene="nadD"
                     /locus_tag="ENN56_03385"
     CDS             1273..1914
                     /gene="nadD"
                     /locus_tag="ENN56_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDS74562.1"
                     /translation="MTVIPDTAGIRRLGLLGGTFDPIHEGHLALADYAAYHAGLDRVW
                     FIPSATPPHKRRSDLADAAIRFEMTRAAVEPYAPRFAATDIELQRDGPSYTVETLRQI
                     RDAVGCETELFWIVGRDNLDEIPQWYQSRRIVEYVTIIAGGRPGERVPDDLPDWFCAR
                     LLLLDGPTIDVSSTRIREQAASGVIDLMSVPEPVARIIQSRGLYGYTAPNTTA"
     gene            2081..>2866
                     /locus_tag="ENN56_03390"
     CDS             2081..>2866
                     /locus_tag="ENN56_03390"
                     /inference="COORDINATES: protein
                     motif:HMM:PF07715.13,HMM:PF13715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS74563.1"
                     /translation="MGRSVHWTLFCTVFATLLIGTAAPAATISGYVREQGTRESMPYV
                     SVFIEGLRLGAMTNDRGYYVINGVPPGEHTLVASLIGYRDYRETLSVGEDALVRNIEV
                     ASEAIEIEALHVEAERVRSPSLDVTPSRETFRGSELKIAPAAVEADPIRTLQTLPGVM
                     QLSDFNTGLYVRGGTPDQNLILVDGAELYNVSHFFGLFSTFPADAIKTTELLMGGFPA
                     QYGGRLSSVLNVVTDEGNKEEVHGVGGVSILSSRLTMEGPMGKG"
BASE COUNT          617 a          832 c          816 g          601 t
ORIGIN      
        1 aaatcgcgga aacgtatctg atcgccgccg aagagctcgt cgcgttgatg cctgacagca
       61 tccgatcggt aacagtcgac gcggtagatg attcgctcgg atacgttcgg atcgcactcg
      121 agtctgcact cgcgaaccgc ggaatacgcg tgctgacgag tgcagcggtg aacacggcga
      181 ttctttccgt cacgacggag cggctcggcg tcaccgtgtc cgatacctgg cggcggacgg
      241 atgtcggccg gcgtgaagtg gaacgtcacg ttgacgtcga actcgcggca cagcttgttt
      301 ttgccgatgc ggttgtctgg cgtcagcacg caccagtcga gacgcataca cgggtgtccg
      361 caacggaact cgcaagtttc gcggacccac gtattcctgt tgaagttcca cgcgataacg
      421 tcatatttgc gttggagacc gcggtgatca tgagttcgat tatcgtggtg ctataccttt
      481 tctttacaca gtgagatcga tgatacgata ccgcacgatt ctcatcggat tcgcgacgat
      541 tctcgtcgcc gtaacactcg tagccaatac cgggtgcgcg cggaaggctc ggcgtctgtt
      601 gacctgcgag gaatactatg acctcggcat gcgtcatctc gagggcaaaa agtggttgaa
      661 agcgcaggag atgttcgagg cgatcacgta caactatcct ggttgtgatc tcgttgatga
      721 cgcacagtac atgctcggtg agacgtactt ccggcagggt ttgtatatcg aagctcagtt
      781 tgagtttcga cgcgtcgtcg aggacttccg tctatccgac cgtatggagg acgcgcagta
      841 tcacatggcg ttggccgcgc acaaacagtc acatccggct gcgctcgatc agacgcctac
      901 tgaagaggcc atcttccggt ttcagcagta tctcgacgac ttccccaacg ggaaatatgc
      961 cgacgaagca cgtgggtaca ttcgtgaagg acgaaagaag ctcgcccaga aagactacac
     1021 ggctgggcgc ttctaccatc gccttgacta cgaagaagct gcgctcatct acctcaatca
     1081 tgtgatcaac gagtatccgg actcggagga atgggtcgag cgcgcacggt atctcaagtc
     1141 ggtgattctc gttgaacgcg atcaatcgca ggaagcgctc gtactgcttc gcacgatcaa
     1201 tcaggatcat atcaaaccga agcttcgtga ggacatacag cgcctcatca cgcggattca
     1261 aaacccggaa cggtgacggt gattccggat acggcgggaa ttcgacgact cgggctcctc
     1321 ggcgggacgt tcgacccgat tcacgagggt cacctcgcac tcgccgacta cgcggcgtac
     1381 catgccggac ttgaccgcgt ctggttcatc ccatcggcga ccccgccaca caaacgccgc
     1441 tccgacctcg cagatgccgc tattcgattc gagatgacgc gcgccgcagt tgaaccgtat
     1501 gcgccgcgct ttgccgcaac cgatattgag ctgcaacgag acggtccgtc gtatacggtg
     1561 gaaacgctcc gacagattcg tgacgcggta ggatgtgaga cggagctgtt ctggatcgtc
     1621 ggacgcgaca atcttgacga gatcccgcaa tggtatcagt cgcgccgcat tgttgagtac
     1681 gtaacgatta tcgccggtgg acgcccgggc gagcgcgtcc cggacgacct ccccgattgg
     1741 ttttgcgcac gactcctcct tctcgacggc cccacaatcg acgtctcttc aacacggata
     1801 cgcgaacaag ccgcctccgg cgtcatcgat ctcatgagtg tacccgagcc ggtcgctcgg
     1861 atcattcaat cgcgcggcct ctacggatac accgcgccaa ataccaccgc ctaaagacct
     1921 cgccaccctt tcgcctcgtc cgtgaattcc ctacagtttt cacagcagag gtgtaacatt
     1981 tctcctcgtt tactcgtctg accgtttgat ccaccggtac tgaatactct acacatgaag
     2041 aatcgccatc gttgtgcaac caaattcgcg cgaggttccg atgggacgat cagttcattg
     2101 gacgcttttc tgcacggtgt tcgcgacatt actcattggc acggcggcgc ccgctgcaac
     2161 aattagcggc tacgtgcggg aacaaggaac tcgagagagc atgccctatg tgagcgtgtt
     2221 tatcgaggga ctacgtctcg gcgcgatgac aaacgaccgt ggatactacg tgatcaacgg
     2281 ggttccgccg ggtgagcaca cactcgtcgc ttcgttaatc gggtaccgcg attaccgcga
     2341 aaccctatcc gtcggtgaag acgccctcgt acgaaatatc gaggtcgcgt ctgaagcgat
     2401 cgagatcgag gcgctgcacg tcgaagcgga acgggtgcgg tcaccttcgc ttgatgtcac
     2461 gccgagtcgg gaaacattcc gcggatccga gctcaagatc gctccggcgg ccgtggaagc
     2521 ggatccgatt cgtacgctcc aaacgctgcc aggcgtcatg caactgagcg atttcaatac
     2581 gggcctctac gtgcgaggcg gcacacccga tcagaatctc atcctcgtcg acggtgcgga
     2641 gctgtataac gtctcccact tcttcggact gttctccaca tttcccgcgg acgctatcaa
     2701 gacaaccgaa ttgctcatgg gaggattccc ggcgcagtat ggcggtcgcc tcagttcggt
     2761 gctgaacgtg gttaccgacg aagggaataa ggaagaagtg cacggcgtcg gcggcgtgag
     2821 catcctcagt tcgcgcctaa cgatggaagg tccgatgggg aagggc
//
LOCUS       DSCC01000275            2879 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023632, whole genome shotgun sequence.
ACCESSION   DSCC01000275 DSCC01000000
VERSION     DSCC01000275.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023632"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            120..1403
                     /locus_tag="ENN56_03395"
     CDS             120..1403
                     /locus_tag="ENN56_03395"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74564.1"
                     /translation="MRQSRVRLFVSIAIVVVSTAIFATAGILIDRRLIGVLDARLGDA
                     LESQARTIATTIIIPPNDDPVSLALIVEDLTTARIASDAQLVVLFDRFGTVIAADPPA
                     LGAEPYLRLDNTALAIALTGDVSAGERYQIGGQDLKSAFAPVTGPFGDVVGVIGIEAP
                     ADYFGALRDVRAALVLTLGIGMALVIGLTLVVWRFWERSERSERAIFRAQQLATIGQM
                     TATMAHEVRNPLSIIRATAERIKRKYSDGDEIFDFIPEEIDRLDRLTRWYLDFARPSE
                     ATVTAVDLRNLVLESLSRVRKEIEAAGIEVDVADTDAPVMVAADRDRLLQAILNIVIN
                     AIEAMPDGGRIDGRILIRGDRARLEIRDSGPGIDAEVHASVFEPFVTTKTTGSGLGLA
                     VVRQVVESLLGRVGIESETGVGTTVWIELPLAKPS"
     gene            1499..1870
                     /locus_tag="ENN56_03400"
     CDS             1499..1870
                     /locus_tag="ENN56_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74565.1"
                     /translation="MRRDKVNTIVSIGNGLCRVIVRSEKLARRLGRIPGVTLREDDAE
                     NLGWWVIFQEELRPIIEPVFEKDHQQTVQGRGKRLVQSSLFDDLRDHPDGDKLWAAET
                     KGLDSDGEDEEDDDGTHSSRR"
     gene            1830..>2879
                     /locus_tag="ENN56_03405"
     CDS             1830..>2879
                     /locus_tag="ENN56_03405"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS74566.1"
                     /translation="MRRTTMARILLVDDERKLGVVLAGDLRDAAHEVLTVQDGRSAIN
                     LLREQSFDVVVTDIRMEPVDGLAVLTETKRLCPDTEVIMMTAYSSTESAIEALRQGAA
                     DYLLKPFRSEELIHTVGRVVERQRLRLENTQLKRDISPPKSAMIAESAQMKEIARIVE
                     RVAKTDTTVLITGPSGSGKEVVAQAIHAQSGRSDGPFVAVNCAALAESLLESELFGHE
                     KGAFTGADKRRLGRFEIADGGTLLLDEIGEIGQSVQVKLLRALETKSFERVGGTESIT
                     TDVRIVAATNKNMEDAVADGRFREDLYYRLNVFPIEVPPLRERPADVRALAIHLISTT
                     DLTLSDTALDVLTCYD"
BASE COUNT          613 a          806 c          873 g          587 t
ORIGIN      
        1 ggcatggcga cgactacgtc gcaacccaca tttacacacg atggtcggtt tgccgagaag
       61 gaagcatatt taccgttgaa cagaatcgct tctctggcta ccagtgaccg gagttgccca
      121 tgagacagtc gagggtgcgc cttttcgtca gtatcgcaat cgttgtcgtc tcgacggcaa
      181 tattcgcaac cgctgggatc cttatcgacc gacgcctcat tggcgtcctc gatgcgcgac
      241 tcggagacgc gcttgaatcg caagcgcgaa ccatcgcgac aacgatcatc atcccgccga
      301 atgatgatcc ggtatcgctt gcacttatcg tcgaagacct tacgacggcg cgaatcgcga
      361 gtgacgcaca gcttgtcgtg ttgttcgacc ggttcggcac ggtaatcgcc gcagatcctc
      421 cggcgctcgg tgctgaaccc tatctccgtc tcgacaacac ggcgctggcg attgcactga
      481 ccggcgatgt ctcagccggt gagcggtacc aaatcggtgg acaggaccta aagagcgcgt
      541 ttgcaccggt taccgggccg ttcggggatg ttgtcggcgt tatcggcatc gaagcacccg
      601 ccgactattt cggcgcgctg cgcgatgtcc gtgcggcact cgtcttgacg ctcggcatcg
      661 gcatggcgct cgtgataggt ctgacactgg ttgtgtggcg gttctgggag cggtccgagc
      721 gctccgaacg agcgatattc cgcgcgcagc aactcgcgac aatcggtcag atgaccgcaa
      781 ccatggcgca cgaggtgcga aatcccctca gtatcattcg cgcgactgca gagaggataa
      841 aacggaagta cagcgacggc gacgagatct tcgacttcat tcccgaagaa atcgaccgac
      901 tcgaccgtct tacgcgatgg tatctcgatt ttgcacgacc gtcggaggcg acagtgacag
      961 cggtcgatct gcggaatctc gtgcttgagt ccctcagtcg tgtgcggaag gagatcgaag
     1021 ccgccggaat cgaggttgac gttgctgata cggatgcgcc ggtaatggtg gcggctgatc
     1081 gcgacagact gctgcaggcg atcctcaata tcgtcatcaa cgcaatcgaa gcgatgcccg
     1141 acggggggcg tatcgatggg cgaatcctca ttcgcggcga tcgtgcacgc ctcgagatac
     1201 gtgactccgg cccgggaatc gacgccgagg tccacgcttc ggtgttcgag ccgttcgtta
     1261 cgacgaaaac taccggtagc ggtcttggcc ttgccgttgt tcgtcaggtt gtcgaatcgc
     1321 tccttggccg cgttggtatc gagagcgaga caggtgtcgg aacgaccgtc tggattgagc
     1381 ttccgcttgc gaagccgtcg taaggcatcg tgcgtcacga gcacggagta acccttctct
     1441 acgtgatgta cgaataggta catttacctc ggaaatccca ccgaatcgcg gagtatacat
     1501 gcgtcgtgac aaagtgaaca cgattgtttc catcggaaac ggtttgtgcc gagtgatcgt
     1561 gcggagcgaa aagcttgcgc gacggctcgg tcgcattccc ggagtgacat tgcgcgaaga
     1621 cgacgctgaa aaccttggat ggtgggtgat cttccaagaa gagcttcgac cgatcatcga
     1681 gcccgtgttc gaaaaggacc atcagcagac cgtgcagggt cgcggcaagc gactggttca
     1741 gtcatcgtta ttcgatgacc tgcgcgatca cccggacggt gacaaactct gggcggctga
     1801 aaccaagggg ttagattccg acggagagga tgaggaggac gacgatggca cgcattcttc
     1861 tcgtcgatga tgaacgcaaa ctcggcgttg ttctcgctgg cgatctccgt gacgcggccc
     1921 atgaagtgct gacggttcag gacggtcgat ccgccattaa tctccttcgt gaacaatcgt
     1981 tcgacgtggt cgtcaccgac atccgtatgg aaccggtcga cgggctcgcg gtgctcacgg
     2041 aaacgaagcg cttatgccca gataccgaag tcatcatgat gacggcgtat tcgagcaccg
     2101 agtcggcgat agaagcgctt cggcaaggtg cggcggacta cctgcttaag ccgtttcgct
     2161 cggaagaact cattcacacg gttggtcgtg tcgttgaacg gcaacgtcta cgccttgaaa
     2221 acacgcagct caagcgagat atttccccgc caaagagcgc gatgatcgcc gagagcgcgc
     2281 agatgaaaga gattgcgcgc attgtcgaac gggtggcgaa aaccgacacg accgtcctca
     2341 ttacgggtcc gagcggctcc ggcaaggaag ttgtcgccca agccatccac gctcagagtg
     2401 gtcgctccga cgggccgttc gttgccgtaa actgtgccgc tcttgccgaa tcactactcg
     2461 aatccgagct gttcgggcac gagaaaggcg cgtttaccgg tgccgataag cggcgactcg
     2521 gtcggttcga gatcgctgac ggcgggacgt tgttacttga tgaaatcgga gaaatcggtc
     2581 aaagtgtgca ggtcaaactc cttcgtgcgc tcgagacgaa gtcgttcgaa cgcgttggcg
     2641 ggaccgaaag catcacgacg gacgtgcgaa tcgttgcggc gacgaacaag aacatggagg
     2701 acgccgttgc cgacgggaga tttcgcgaag acctgtacta tcggctgaac gtcttcccca
     2761 tcgaagtacc gccgctgcgc gagcgtcccg cggatgtccg cgcactcgcg atccatctca
     2821 tttcgactac ggacctgacg ttgagcgata cagcattgga cgtgctcacg tgctacgac
//
LOCUS       DSCC01000276            2238 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033033, whole genome shotgun sequence.
ACCESSION   DSCC01000276 DSCC01000000
VERSION     DSCC01000276.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033033"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            1747..>2238
                     /locus_tag="ENN56_03410"
     CDS             1747..>2238
                     /locus_tag="ENN56_03410"
                     /inference="COORDINATES: protein motif:HMM:PF14054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HDS74567.1"
                     /translation="MNRCVFRLFMLTASTAVFAGCGIDPVSPNPPEQLIIQALLQPGH
                     PIDNIRLTRTMPPESYYAGIPQSTVSGATVVLYHESRIDTLSELTVGIYGDDTLIARS
                     SITYRVEVTFEDFILRAETTVPEKLEYTAVTTVSGSRYIPSADTLVFPREYADPDQFP
                     PQGN"
BASE COUNT          499 a          621 c          635 g          483 t
ORIGIN      
        1 gaccagtctc tataacgtgt cccacctttt cgggctcttc agcatgttcc ctgcggatgc
       61 ggtggagtcc gccgaactga tgaagggcgg attccccgcg caatatggcg gacgcttgag
      121 ttcggttctc aaggtcacga gccacgacgg caaccgtcag ggcgttaccg gctctggcgg
      181 ggtgagcctt atcagctcgc gcatgacgct gcaggggccg gtcggaaacg gttcgtggat
      241 gctgagcggg cgtcgtacgt atctggagcc gatattggcc attgcggcaa agttcaacag
      301 cggtatcgac gcgttcggat acagcttcta cgatgtgcag ggaaagattc aacagaacat
      361 attcgatcgc gataggctcg aggtttcgtt ttatctcggc gacgacgacc ttgcgtacga
      421 tcagccgaca ctgaacgccg gccaactctg gggcaatcgt gccgcctcgg tcatgtggtc
      481 gcacgtggcg actgacgaca ttgtcacgac gtaccgactc tccgggagtc ggtaccgttc
      541 gcgacttcga ctccagctcg aagatgtcgg agccgtcgaa acgaatcgga tcaccgatat
      601 atctgggaaa accgatatca catggttcgc aaccgacgac ctccgtatcg aggctggtat
      661 cgactttgtg caccacgaca tggactacac gtttgacttc gacgagaacc ggttcaactc
      721 gttcgcaacg aaatcgtgga actggagcgc ttacgcacag gatacgtggt taccgtggcc
      781 gttcctgacg atccagcccg ggatgcgtgc gagttggttc accaatggcg actacgtcac
      841 atggaatcca cggctttcga cgcgatatca ggtcggagat aatacatttt tcaaagccgc
      901 aatcggacga tattcgcaac atctcttccg cgtgtcgcgg gaaacacagg gactttcgat
      961 cttcagcgat ctctggttca ccgccgattc gaccgcgggg ccaagtcacg cgtggcagta
     1021 tatcgccggg ttcgaaacac ggctcggtcg cgattgggac ctcgatatcg aagtgtacta
     1081 caaggatttt gaacggctcg ctgagttcaa taacggcatc ggtcccggtg aaccgacgat
     1141 tggtgagctg ttacttcgcg gcgaaggcga agcgtatggc cttgaggttg gggtgaagaa
     1201 acgcgcgggc gagcataccg gatgggtatc ttactcactg ggatgggcaa cacggacgat
     1261 cgccggcatc aacacggtgg aatcgcgtga tcgtgattca gagccgcaac cgtactatcc
     1321 gaagttcgat caacggcatt cattgaacgt tgtgtaccag tggcaattca ccgagcgctg
     1381 gacattcaat tcgagctatg cattcgcctc cgggcagccg tatacaccgc tgcttggtcg
     1441 gtatgagatg aacgacgacc tcgtcacgtg ggaacaaacc tatacggcac ggacaaacag
     1501 cgttcgcttg ccgtactact cacggctcga ccttggtgtt cgtgccaact accgcggatg
     1561 gggcatcacg tggcagccat tcgtccaggc aattaacgtg ttgaatcgga aaaacatctt
     1621 caatcggtac tggaccgacg gcgatgcgga agccgtaccg ccgaaacccg gtacagagac
     1681 gaacttcccg caattaccgt tcctcgtcac gctcggcgtg gacgtaatgt tctgaaagat
     1741 agacgaatga atcgctgtgt tttccggttg tttatgctta cagcttcaac ggcagtcttc
     1801 gccggttgtg gaatcgaccc ggtcagtccg aatccgccgg agcaactcat catacaggcg
     1861 ctgcttcagc ccggtcatcc aatcgacaac attcgtttga cccgtacgat gccgcctgag
     1921 agttactacg cgggcatccc gcaaagcacg gtgagcggcg cgacagtcgt gctctatcac
     1981 gaatcgcgga tagatacgct gagtgaactg acggtgggaa tctatggcga tgacacactt
     2041 attgcgcggt ccagcataac gtatcgcgtt gaagtcacgt tcgaggattt cattctccgc
     2101 gccgaaacga ccgtgccgga aaagcttgaa tacacggcag ttaccaccgt cagcgggagt
     2161 cggtatatcc cctccgccga tacgctcgtc tttccgcgcg aatatgccga tcccgatcag
     2221 tttccaccac agggaaac
//
LOCUS       DSCC01000277            1677 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048922, whole genome shotgun sequence.
ACCESSION   DSCC01000277 DSCC01000000
VERSION     DSCC01000277.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048922"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..766)
                     /locus_tag="ENN56_03415"
     CDS             complement(<1..766)
                     /locus_tag="ENN56_03415"
                     /inference="COORDINATES: protein motif:HMM:PF01408.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74568.1"
                     /translation="MPDTLTWGLISTARITRKIIPAIQSSPACTLMAIASRDIEKAHA
                     FAAEYGIPRSYGSYEELIDDPKIDCIYNPLPNSMHADWTIRAVEAGKHVLVEKPFTMD
                     AEEAEDVFDTARAKRRIVMEAFMYRFHPQWERVRRMIDNGDIGELKFIRAQMGGHMNR
                     LDDIRMVPDMGGGALMDIGCYCLNACRMVTGREPEWVSATANYENGVDVMFAALMRFP
                     GNVLAQFNCGFRSNPHNDVDIIGTDASIRVARPWGPD"
     gene            complement(868..>1677)
                     /locus_tag="ENN56_03420"
     CDS             complement(868..>1677)
                     /locus_tag="ENN56_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HDS74569.1"
                     /translation="VAIIPGRRVQIHTKNGVVKGVIGKRAIHLMSDEERNKPKVEIES
                     FWIDIAVENKDEALELVAIGDPATYDVGFDELRNGIATSRAFDDKAGAFVVAETLRIL
                     AENRGALKAAVYGVSTVQEEIGLRGATTAAFGVDPQVAVAIDVTHATDSPDMDKRKVG
                     EVKLGGGPVITRGANINPVVFDRLVQAASMKTNGDTPAYQIDAAPRGTGTDANAIQLT
                     RAGVAAGLVSIPLRYMHTAVETVALTDLESSAQLLATFAQAVEPDTSFIPA"
BASE COUNT          327 a          496 c          461 g          393 t
ORIGIN      
        1 ggtctggacc ccatgggcgg gcgactcgaa tcgacgcgtc ggttccgata atgtccacgt
       61 cgttatgcgg attgcttcgg aagccgcagt tgaactgcgc gagcacgttt cccgggaacc
      121 gcataagcgc cgcaaacatc acgtcgacgc cgttctcgta gtttgccgta gcagaaaccc
      181 attccggctc gcgtccagtg accattcggc acgcgttcag gcaatagcac ccgatgtcca
      241 tgagtgcgcc accacccata tccggcacca tacggatatc gtcaagacgg ttcatatggc
      301 cgcccatctg cgcgcgaatg aacttgagtt caccgatgtc accgttgtca atcatccggc
      361 ggacgcgttc ccattgaggg tggaaacggt acataaacgc ttccatcacg atacgacgtt
      421 ttgctcgggc cgtatcgaaa acatcttccg cttcttccgc atccatcgtg aacggtttct
      481 cgacgaggac gtgcttaccg gcctctactg cacgaatcgt ccagtctgcg tgcatcgagt
      541 ttggcagtgg attgtagatg caatcgattt tcgggtcgtc aataagctct tcgtagctac
      601 cgtatgagcg aggaataccg tattcagctg cgaaggcgtg cgctttctct atgtcgcggc
      661 tggcgatcgc cattagtgta caagccggcg aagattggat agccgggatg atttttcgtg
      721 ttatgcgagc agtactaatg agtccccacg tgagcgtgtc tggcatatcg atccctccgt
      781 ggcgggtgtc ctgtgaacaa tcgcgggggt cacgaaaccg tgtcgcgccc cccgtcgatc
      841 agcaatcggt ttctgagagt cggtatttca tgctggtata aaactcgtat ccggttcaac
      901 ggcctgcgca aaggtcgcaa gaagctgcgc cgagctttcg agatcggtaa gtgcgaccgt
      961 ttccaccgct gtgtgcatat atcgcaacgg aatactcacg agccccgcgg ccacacctgc
     1021 gcgagtaagc tgaatcgcgt tcgcgtccgt tccggttcca cgtggcgccg catcgatctg
     1081 gtacgccggg gtgtcgccgt tcgttttcat cgaggctgct tgaacgagac gatcgaacac
     1141 aacgggattg atattcgcgc cgcgggtaat cacgggaccg ccgccaagtt tgacttctcc
     1201 gactttgcgc ttgtccatgt ccggggagtc cgtcgcgtgt gtgacgtcaa tcgctaccgc
     1261 aacctgcgga tcgactccga atgcagcggt agtcgcgcca cgcaagccaa tctcttcctg
     1321 cacggtgctc actccgtata cggccgcttt gagtgcgccg cgattctctg cgagtatacg
     1381 gagcgtttcc gcgacgacaa acgcaccggc cttgtcgtca aacgcacggg aagtcgcgat
     1441 tccattgcgc agctcatcga aaccgacgtc gtacgttgcc ggatcaccta tcgccacgag
     1501 ttcaagcgct tcgtccttat tctccacggc gatgtcgatc cagaagctct cgatttccac
     1561 tttcggcttg tttcgctcct cgtcggacat gaggtgtatc gcgcgtttcc cgattacacc
     1621 ctttacgacg ccgtttttcg tgtgaatctg aacgcgacgt ccgggaatga tggcgac
//
LOCUS       DSCC01000278            4634 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1012448, whole genome shotgun sequence.
ACCESSION   DSCC01000278 DSCC01000000
VERSION     DSCC01000278.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1012448"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            66..1418
                     /locus_tag="ENN56_03425"
     CDS             66..1418
                     /locus_tag="ENN56_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDS74570.1"
                     /translation="MPTDAHHHVILDSLNEAVFTVDLDWRVTTFNRAAEQITGIDRED
                     AIGRPCWEVFRASVCESSCPLKRTFSTNRPTENARAYIVTPAGKRVPIRISAAILRDE
                     DGEVIGGVETFQDLSRIEELEKELRARYTFEDIVGRSPAMVNLFEILPQIAASDSTVL
                     IEGASGTGKELFARAIHNLSPRKRKRFVAVNCGALPDTLFESELFGYKAGAFTDARRD
                     KPGRFALAEGGTIFLDEIGDISPAMQSRLLRVLQERTYEPLGGVDSVKADVRVVAATN
                     RNLAERTAEGKFREDLFYRIRVVRINLPPLRERREDIPLLAEHFVDKYARLTGKSIEG
                     VTDAVMARFVEYQWPGNVRELENIIEQAFVMCNGSLIDLHHLPPELRPSDLERSAHLT
                     PRTSLESMERLMIEEALRRNNGNRRRSAADLGIDPSTLYRKMKSLGIEPPPTDGRQRV
                     "
     gene            1582..2412
                     /locus_tag="ENN56_03430"
     CDS             1582..2412
                     /locus_tag="ENN56_03430"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS74571.1"
                     /translation="MPMLRITVLSENTAGGSNLLAEHGLALWIERNQRRFLFDTGQGL
                     VLGWNARVLNIDLDTVDAVILSHGHYDHTGGLPQVLSEHRTTPIYAHPAAFGSKFSVR
                     PNSKTRDIGIPSDAASAVRAHARVISVMGPTDLGEGLYLTGPIPRLNDFEDVGGPFYR
                     DAEGRVPDDLIDDQALYHDGADGVVVILGCAHSGVVNTLDYVRKLTGGKPFRAVIGGM
                     HLVSAKPERVDRTIHALRDYDVATLAPMHCTGPEATGAIRSAFPSACIALPTGATLAL
                     "
     gene            2657..>4634
                     /locus_tag="ENN56_03435"
     CDS             2657..>4634
                     /locus_tag="ENN56_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74572.1"
                     /translation="MYSLRNQSTRAALAAVYAALLFATGGAGADTLVSGQVQTGSWAQ
                     ANSPYRVVGTITVPPGHSLTIEPGVDVLFDANVQFIVQGALLAVGTSTDSIRFLPNTD
                     VQITQWAGIRISGGETSRFHYVRMSGANATGSAPDDRGGAIYAFDDGTTIDVENSVFS
                     GNRAATSGGALYVTNGASITLVNTMVIGNTAANGTGGGLAVLTGQGNIANTTIARNSA
                     SGQGGGAYASASSLTITSSIFEWNTATGAGGGLLLSGSGASIVSAVFDTNSSGNDGGA
                     ICLYNASTVDISSSLIERNKSSDEGAGIFANAQSIVTIDDTPIRRNRSSAYYGGGGIY
                     VDNATLTMTSSVIEENYTTGAYGSGAGIRASNSTVRVFDSPLTGNRATGSNGNGGGLY
                     AYSCDLILSRSPVTNNFANDEGGGIALWNYGTAIVKDSPVRENEAGTLNGGGVRVYNR
                     PNVTFDNSPIVGNTATLLNGGGIAVTAYSNVRLIDSPVDSNFAGQHGGGVAVWDNGIL
                     RLTNSSVDGNESAQDGGGIYIEAAALTMLNCSVTRNTGVHGGAFRLVNTGTGTKTVSL
                     MNVTATENVATGTGGGIRAFQASATIMNSIFWNNLPIEFYTDESTISATYSDIAYEFD
                     VWPGDGNINIDPLFTDPFNAVWFPGIGSPAIDAGN"
BASE COUNT         1039 a         1343 c         1329 g          923 t
ORIGIN      
        1 atatgcaaca cttaccacgt actattctca gtagcgcacg aattcgtggg gaatccggag
       61 cgaacatgcc tacagatgca caccatcacg tgattctcga ttcgctcaac gaggccgtct
      121 tcaccgtcga cctcgattgg cgcgttacca cgttcaatcg tgccgcggag cagatcaccg
      181 gtatcgaccg cgaggatgca atcggcaggc cgtgttggga ggtgttccgc gcgagtgtct
      241 gcgagagctc gtgcccgttg aaacgcacgt tctccacaaa ccgaccgaca gaaaacgcac
      301 gcgcatatat cgtcacgccc gcgggcaaac gagtcccgat ccgcatctcc gccgccatcc
      361 ttcgcgacga agatggcgaa gtgattggtg gagtcgaaac gttccaagac ctaagccgga
      421 tcgaagaact ggaaaaggaa cttcgcgcgc gctacacctt cgaggacatc gtcggtcgta
      481 gcccggcgat ggtgaacctg ttcgaaatac ttccgcagat tgcggcaagt gatagcaccg
      541 tgctgatcga gggagcaagt ggcaccggca aggaactgtt cgcccgggcg atccataacc
      601 tctcgccgcg caagcgaaag cgattcgttg cagtcaactg cggagcattg cccgacacac
      661 tcttcgagtc ggaactgttc ggatacaaag cgggcgcctt caccgatgcg cgacgtgata
      721 aaccgggacg cttcgccctt gcggaaggtg gaacaatctt tctcgacgag atcggcgaca
      781 taagcccggc gatgcaaagt cgattgctcc gcgttctcca ggagcgcacc tacgagccac
      841 ttggcggcgt cgactcggta aaagccgatg tccgggttgt cgccgcaaca aatcgtaacc
      901 tcgccgaacg taccgcagaa gggaagttcc gcgaagacct cttctatcgt atccgcgtgg
      961 tccgaatcaa tctgccaccg cttcgtgagc gaagggaaga cattccgctc ctcgccgagc
     1021 acttcgtcga caaatacgct cggctgaccg gaaagtcgat cgaaggcgta accgatgccg
     1081 tcatggcgag gtttgtcgag taccaatggc cgggcaatgt gcgagagctc gaaaacatca
     1141 tcgagcaggc gtttgtgatg tgcaacggat cgttgatcga tctccatcac ctcccgcccg
     1201 aacttcgccc gtcggatctt gagcgaagcg cgcaccttac cccccgaacg tcacttgaat
     1261 cgatggaacg tctcatgatc gaagaggcac tccgacggaa caacggaaac cgacgtcgct
     1321 ccgccgcgga tcttggaatc gatccgagca cgttgtaccg gaagatgaaa tcactgggta
     1381 tcgaaccacc accaaccgac ggtcggcaac gcgtatagga ctattgcatt tcgcaatgga
     1441 atccattgca ctccaccttc tcggtacgtg tcgattgcgt gtttacgacg tttgtgcggg
     1501 cgttggcatg gtggttgccg tgaaacaatt tgcccgtcga aaagcaggtt aatctcacgc
     1561 cataacgaac gaaaagcgaa ggtgcccatg ttgcgtatca ccgttttatc cgagaatacg
     1621 gccggcggct ccaacctcct cgctgaacac ggtttggcgt tgtggattga gcgcaaccaa
     1681 cgacggtttc ttttcgacac cggtcaggga ttagtactcg gatggaacgc gcgcgtgttg
     1741 aacatcgatc tcgacacagt cgatgcggtg attctcagtc acggccatta cgatcacacc
     1801 ggtggactcc ctcaagtgct ttcagagcat cgcacaacac cgatctacgc acaccctgcg
     1861 gcgtttggat cgaaattcag tgtgagaccg aactccaaga cccgggacat cggtataccg
     1921 tcggacgccg cgagtgccgt acgcgcgcac gcacgcgtca tttctgtaat ggggccgact
     1981 gatcttggtg agggattgta tcttaccgga ccgattccgc gtctgaacga tttcgaagac
     2041 gtcggtgggc cgttttaccg tgatgccgag gggcgtgtgc ccgacgacct catcgacgac
     2101 caggctctct accacgatgg cgccgacggc gtagtcgtga tattgggttg cgcgcattcc
     2161 ggtgtagtga acacgctcga ctacgttcgg aagttgaccg gtggcaaacc cttccgcgcg
     2221 gtaatcggcg ggatgcacct cgtttcagca aaacccgaac gagtggaccg cacgattcat
     2281 gcacttcgcg attacgatgt ggccacactc gcgccgatgc attgtaccgg ccccgaagct
     2341 accggggcaa tccggtcggc tttccccagc gcatgtatcg cgctgccaac cggtgcaaca
     2401 ctcgcgttgt aggcgctcca cgtacctcga ctatgcacgt agctcggatt cacacggaat
     2461 ccgagcgagt cgtgcactct cacctcacac tacctcacgc tgtccggtgt atatttgagc
     2521 gacgagtcat tatctcgcag atctgaatca gctaatagag agaggggcat cactgagagg
     2581 gtgatggtgc gacgttcttg atgttttttt tcgactgctg ggtccagcta caagccgtaa
     2641 ttgggggaag acaccgatgt attcgctgag gaatcagtct acgcgtgcag cgcttgccgc
     2701 cgtatacgcg gcacttcttt tcgccaccgg cggcgcagga gccgacacgc ttgtctccgg
     2761 gcaagtgcag accgggtcgt gggcacaggc gaactcaccg taccgcgtcg tcgggacgat
     2821 caccgtcccg cctggtcact cactaacgat cgaacccggc gttgatgttc tcttcgacgc
     2881 gaatgtccag ttcatcgtgc agggagcact ccttgccgtc gggacctcca ccgatagcat
     2941 tcgctttctc ccgaacacgg atgtccaaat cactcaatgg gcgggtatcc gcatcagcgg
     3001 cggtgaaacg agtcggttcc actacgtccg aatgagcggc gcaaatgcga caggttcggc
     3061 accggatgac cgcggtggag cgatttacgc ctttgacgac ggcacgacca tcgatgtgga
     3121 gaacagcgtg ttcagcggaa accgagccgc aacgagcggc ggtgcgctgt acgtgacgaa
     3181 cggcgcgtcg atcaccctgg taaacacaat ggtgatcggc aacacggcgg caaacgggac
     3241 cggcggaggt ctcgcagtgc ttaccgggca ggggaatatc gcgaacacga ccatcgcccg
     3301 gaactccgcg tcgggccaag gtggcggtgc ctacgcaagc gcctcgagcc tcacgatcac
     3361 gagttccatc ttcgaatgga acacggcaac aggagcgggt ggcggtcttc tcctcagcgg
     3421 ctcgggtgca tcgattgtca gtgccgtctt cgatacaaac agtagcggaa acgacggcgg
     3481 agccatctgc ctgtacaacg cttccaccgt agatatctca tcgtcactca ttgaacggaa
     3541 caagtcgtcc gacgaaggcg cgggaatctt cgcgaacgca cagtcgatcg tgacgattga
     3601 cgatacgccg attcgtcgga acagaagctc ggcgtactac ggtggcggcg gcatttacgt
     3661 cgataatgca acgttgacga tgacgagttc cgttatcgaa gagaactaca ccacgggagc
     3721 gtacgggagc ggcgccggca ttcgcgcatc caattcgacg gtgcgcgttt tcgattcacc
     3781 gctcaccgga aaccgtgcga cggggagtaa cggaaatggt ggtggtctgt acgcctatag
     3841 ctgcgacctc atcctatcga gatcacccgt caccaacaac ttcgcaaatg acgaaggtgg
     3901 gggtatcgcg ctctggaact acggaaccgc gatcgtcaag gacagtccgg ttcgcgagaa
     3961 tgaggcggga acgctgaacg gtggcggcgt tcgagtctac aaccgtccaa acgtgacgtt
     4021 cgacaacagc cccattgtcg gcaacacggc gacactcctg aatggtggag gcatcgccgt
     4081 taccgcgtat tccaacgtgc ggcttatcga ctcaccggtg gacagcaact tcgcgggaca
     4141 acatggaggc ggagtcgcag tatgggataa cggaatcctg cgtttgacga attcctccgt
     4201 ggacggaaat gaatcagcac aagatggcgg cggcatatat atcgaggcgg cggcactcac
     4261 catgctgaac tgctcggtca cgaggaatac cggcgttcac ggcggagcgt ttcgactcgt
     4321 gaatacagga actggtacga aaaccgtgag cctgatgaat gtcacagcga ccgagaacgt
     4381 tgctaccggt acaggtggtg gcattcgtgc gtttcaagcg tcggcgacaa tcatgaactc
     4441 catcttttgg aataatctcc ccatcgagtt ctacacggat gagagcacga tctcggccac
     4501 gtattccgat atcgcatacg aattcgatgt ctggcccggc gacggcaata tcaacatcga
     4561 tcccctgttt accgacccgt tcaacgccgt atggtttccc ggtatcggtt ctccggcaat
     4621 cgacgcagga aatc
//
LOCUS       DSCC01000279            3079 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021548, whole genome shotgun sequence.
ACCESSION   DSCC01000279 DSCC01000000
VERSION     DSCC01000279.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021548"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..304)
                     /locus_tag="ENN56_03440"
     CDS             complement(<1..304)
                     /locus_tag="ENN56_03440"
                     /inference="COORDINATES: protein motif:HMM:PF00675.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS74573.1"
                     /translation="MTTSASIDPQTLPTADTVSRYTLPSGAVLLVRRNPTTPSVIIHT
                     VVRAGGVDEPPQRTGLARFTAEMLARGTETRSATQLWETAESLGASFDVSSSKHTLQ"
     gene            complement(401..1381)
                     /locus_tag="ENN56_03445"
     CDS             complement(401..1381)
                     /locus_tag="ENN56_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_430795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDS74574.1"
                     /translation="MASWNVYVTRQIPDAGLKLLRDAGIDFDMNPHDRVLTRDELLAG
                     VRGRDAVLCLLTDTIDAEVLDASRGARIFANYAVGFNNVDVAAATERGVLVTNTPGVL
                     TDTTADMAWALMFAAGRRIAEADRFTRAGKFEGWGPMLLLGQDITGATLGIVGPGRIG
                     TAVAQRAKGFNMRVLIAGPDPSEEILGIADEQVSLDELLRESDFVSLHVPLVKETHHL
                     IGERELQLMKPTAYLINTSRGPVVDEKALVHALRDHVIAGAGLDVFEDEPALAPGLAE
                     LENVVIAPHIASATNATRSKMATMAAENLLAGLRGETPPNLVNPEVLAKR"
     gene            complement(1431..2576)
                     /gene="galK"
                     /locus_tag="ENN56_03450"
     CDS             complement(1431..2576)
                     /gene="galK"
                     /locus_tag="ENN56_03450"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDS74575.1"
                     /translation="MERAVQLHRDTWNAEPDVIASAPGRVNLIGEHTDYNDGFVLPMA
                     IDRRTVVALSKTEGKDAVFVSEHHPAPVEVIGPSWQPINDPTWVNYPVGVIDGIERHT
                     GLRGNGLRISVASDVPIGSGLSSSAALEAATASAVLGRLDVKLPKSDVAAICQRAEHE
                     FAGVRCGIMDQMASVLGAIILLDCRSLEVDLIALPSDVSVVILDSGIPRGLSQSAYNE
                     RRGQCEDGVAKMQQRWPEIRALRDVTPRMLDRAVDLLPEVVARRCRHVVTENERVIEA
                     ADALRAGDAQRFGELMWQSHESMRDDYEISLPEIDSLVERAMASDGCYGARLTGAGFG
                     GAVVALVKTKRVQEFASTVIAGYNEETGREGTAMLVSADVGARVETS"
     gene            complement(2606..>3079)
                     /locus_tag="ENN56_03455"
     CDS             complement(2606..>3079)
                     /locus_tag="ENN56_03455"
                     /inference="COORDINATES: protein motif:HMM:PF08308.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HDS74576.1"
                     /translation="RYKNHRGEYEWLTQEQIEKRKARRKRSGSKRMRESKSWIPQITS
                     QKLDSLLGTVFSAALVLAIIATAVYLIARSGEVTSPYNVHVASQPPGAAIFIDGAATE
                     KTTDAYIHVKSAGDYEVSVHLPGYRAIPSEIRVDLNDVEPTRSIVFSLYPQEPSE"
BASE COUNT          635 a          902 c          884 g          658 t
ORIGIN      
        1 actgtagtgt gtgcttcgag ctcgatacgt cgaatgacgc accgagtgac tctgctgtct
       61 cccagagttg cgttgccgat cgcgtttccg tccctcgcgc gagcatctcg gcggtgaatc
      121 gagcgaggcc ggtacgctgg ggcggttcat cgactccacc ggctcgcacg accgtgtgaa
      181 taatcaccga cggagtggtc gggttccgcc gcacgaggag tacggcaccc gatggtaagg
      241 tgtagcgaga aaccgtgtct gcggtcggaa gtgtttgtgg gtcgatagac gcgctcgtgg
      301 tcatgcagca aatccgcaag atgattcgtt tgaatcgggt aacgtgaaga taccacccct
      361 cggaacgaac tcgaagggac agtatcgaaa ccggtgacac ttaccgcttc gccaacactt
      421 cgggattcac gagattcggt ggtgtttccc cgcgtagacc agcgagtaga ttttcagcgg
      481 ccatggttgc cattttggac cgcgtcgcgt tcgtcgcact tgcgatatgt ggcgcaatca
      541 ccacgttctc aagttcagcg agcccgggcg cgagtgccgg ttcgtcttcg aacacgtcaa
      601 gacccgcacc ggcgatgacg tgatctcgca gtgcgtgtac aagcgccttt tcgtccacga
      661 cgggaccgcg cgaggtgttg atgaggtatg cggtcggctt catgagttga agctcacgtt
      721 cgccaatgag atggtgtgtc tctttcacaa gcggaacgtg taatgatacg aagtcggatt
      781 cgcgcaaaag ctcgtcgagc gatacctgtt cgtcggcaat accgagtatt tcctcggaag
      841 gatcaggacc ggcgatcagt acacgcatgt tgaagccctt cgcgcgttga gctactgcgg
      901 taccgatacg tcccggcccg acgataccca aggtcgcgcc ggtgatgtct tggccaagaa
      961 gcaacatcgg accccaacct tcaaacttcc ctgcgcgcgt gaaccggtcc gcctcggcga
     1021 tccgacgtcc ggcggcaaac atcagcgccc atgccatatc ggcggtggta tcagtcagta
     1081 ctcccggcgt gttcgtgacg agaacgccgc gctctgtcgc cgcggctaca tcgacgttat
     1141 tgaagccgac ggcgtagttc gcgaatatgc gtgcgccgcg tgacgcatcg aggacttcgg
     1201 catctatcgt atcggtgaga aggcacagca ctgcgtcacg tccacgcaca cccgcgagta
     1261 actcgtcgcg ggtcaggacg cgatcgtgcg gattcatatc gaagtcgatc cctgcgtcgc
     1321 ggagaagctt caatcccgcg tcgggaatct gacgggttac atacacgttc caagatgcca
     1381 ttggtgaaat tcctccatat ggttattcga gaaagaacgc aaacggatga ttacgaggtt
     1441 tcgacgcgtg caccgacatc tgccgatacg agcatcgccg tgccttcgcg acctgtttct
     1501 tcgttgtatc cggcgatcac tgtcgaagca aattcctgta cgcgtttcgt cttcacgagt
     1561 gccacgaccg cgccaccgaa tcctgctccg gtaagtcgag caccgtaaca cccgtcggaa
     1621 gccatggcgc gttcaacgag ggaatcgatt tccggtaggc tgatctcgta atcgtcgcgc
     1681 atactctcgt gagactgcca catcaattcg ccgaatcgtt gcgcgtcacc cgcacgaagc
     1741 gcgtccgccg cctcgatgac gcgttcattt tccgtgacga catggcgaca ccttcgcgca
     1801 acgacctccg gcaacaggtc aaccgctctg tcgagcatcc gtggtgtaac gtctcggagc
     1861 gcacgaattt ccggccaccg ctgctgcatc ttcgcgacgc cgtcctcaca ctgcccgcgg
     1921 cgttcgttgt acgcggattg actcagaccc cgcgggatac cggaatcgag tatcacgaca
     1981 ctcacgtcgc tcgggagtgc gatgagatca acttcgaggc tgcggcaatc gagcagaata
     2041 atcgctccga gtacactcgc catctgatcc atgataccgc atcgtacacc ggcgaactcg
     2101 tgctcagcac gctgacaaat cgcagcgaca tcggatttcg gtagcttaac gtcgagtcgt
     2161 ccgagaacag ccgacgccgt cgccgcttcg agtgcggctg aactactcag gcccgatccg
     2221 atcgggacat cgctcgcgac ggaaatacgt aaaccattac cgcgcaatcc tgtatgtcgc
     2281 tctatgccat caataacacc caccggatag ttcacccacg tcggatcgtt gatgggttgc
     2341 cacgacggac caataacctc gaccggtgcg gggtggtgtt ccgagacaaa taccgcgtcc
     2401 ttgccttcgg tcttgctcaa tgcgacgaca gtgcgacgat cgattgccat cggcagaaca
     2461 aagccgtcgt tatagtcggt gtgctcaccg atgagattga cgcgacccgg cgctgacgcg
     2521 attacgtccg gttccgcgtt ccacgtgtcg cgatggagtt gaacagctct ttcgattacg
     2581 gacggtgaaa cgtgcgcggc agcagtcact cggaaggttc ctgtgggtaa agcgaaaaaa
     2641 cgatcgaccg agtcggttca acgtcgttaa ggtcgactct gatttcggag ggaatggctc
     2701 ggtaccccgg aaggtgaacg gaaacctcat agtcgccggc gcttttcacg tggatgtagg
     2761 cgtcggtcgt cttctcagta gcggcaccgt caatgaaaat tgcggcgccg ggtggttggg
     2821 atgcgacgtg aacgttgtac ggcgacgtca cctcgccaga tcgagcgatc agatagacgg
     2881 ccgtcgcaat gatcgcgagc acgagagcag cggaaaacac cgttccgagt agcgagtcga
     2941 gtttctgact ggtgatctgc ggtatccacg atttactctc gcgcatacgc ttcgaacccg
     3001 accgtttgcg tcgcgctttt cgcttttcga tctgttcctg cgtgagccat tcgtactcgc
     3061 cacgatggtt tttataccg
//
LOCUS       DSCC01000280            2390 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030224, whole genome shotgun sequence.
ACCESSION   DSCC01000280 DSCC01000000
VERSION     DSCC01000280.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030224"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1596
                     /gene="recN"
                     /locus_tag="ENN56_03460"
     CDS             <1..1596
                     /gene="recN"
                     /locus_tag="ENN56_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDS74577.1"
                     /translation="LGTLLGERTSPDVVRAGQDRAIVEARFILPDKHPTLAMVHELGG
                     EIDGNELILRREIIADGRTRVWVGGASVPVRTLRDIGDRLVDFHGQHEHQLLLRPSAH
                     VELLDAFAENAERLAALSESARTLRQVRKRRSELVARKEELATRRETIEFERQELDEI
                     DPQPDELVALETERNVLENVERLGNLLTGLTQLLSEAEDSVVSRLGSGRRWIQEAAEV
                     DESLTDVLAQYEETEVYAQETATRLADRLATLEADPMRLEYVLTRISQLRRLVRRYGS
                     IEEAIEHRRELQQAVDDDAEIDSRIVDIEVKILDEHKRFTQAIRTLTDARQSAANVMS
                     REVTSSLGALGMDHARFEVQLTRTKADSVDPVESDAVEVDGQVYEASPTGAEEVEFLI
                     APNVGEPLRPLVRIASGGEISRTMLAIKSVISEHDPVTTMVFDEIDAGVSGRIAQAVG
                     ERMRELARKRQIVAITHLPQIAAFADEHIVVSKHEHAGRTITEANVITGEARTNALAA
                     LFGGAKVSETALEHARALLGERR"
     gene            1596..2210
                     /locus_tag="ENN56_03465"
     CDS             1596..2210
                     /locus_tag="ENN56_03465"
                     /inference="COORDINATES: protein motif:HMM:PF01066.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDS74578.1"
                     /translation="MDAENTFIRSLVRGVRQKLKVPRGRIFSAGNLVSMSRVALLPFM
                     LSLLKKPAEGKWLVLSIVLAAIIAATDALDGFIARQLNQVSELGRVFDPIADKICIGF
                     VGVWLSTYRGLPVWIPAVIIARDVVIVIGAIVIARKTDIVMPSHQVGRVTTFVLTVTF
                     FIYIIDWQWPQMALVWTSGALIFMSFAVYLRIGWHMLHQRTWIA"
BASE COUNT          524 a          669 c          756 g          441 t
ORIGIN      
        1 ctcggtacgc tcctcggtga gcggacgagt ccggacgtgg tgcgtgcggg ccaggatcgc
       61 gcgatcgtcg aagctcgctt cattcttccc gacaaacatc cgacactcgc tatggttcac
      121 gaactcggcg gcgaaatcga cgggaacgaa ctgattcttc gccgcgagat tatcgccgac
      181 gggcgcacac gcgtctgggt cggcggcgca agcgttcccg tccgcaccct ccgcgatatt
      241 ggcgaccgtc tcgtggattt tcacggtcag cacgaacatc agcttcttct ccgaccctct
      301 gcacatgtcg aactcctcga cgcattcgcc gagaacgcag agcggttggc agcgctctct
      361 gagagcgcgc gtacgctgcg gcaggtacga aaacgccgta gcgaacttgt cgcgcgcaag
      421 gaagaacttg ccacgcgacg cgaaacgatc gaatttgagc ggcaggaact cgacgagatc
      481 gacccacagc cggacgaact cgtcgctctc gaaaccgagc gaaacgttct ggagaacgtc
      541 gagcggctcg ggaatctctt gacgggactc acgcaactac tcagtgaagc ggaagattcc
      601 gtggtgagca ggctcggttc ggggcgtcgg tggatacagg aagccgcgga ggtcgacgaa
      661 tcgctgacag acgtgctcgc tcagtacgaa gaaacggaag tatacgcgca ggaaacggcg
      721 acgcgactcg cggaccgact cgccacactc gaagccgatc cgatgcggct ggaatacgtt
      781 ctgacgcgca tcagccaact ccgtcgcctc gttcgacggt acggatcgat cgaggaagcg
      841 atcgagcatc ggcgcgaact gcaacaggcc gtggacgacg atgccgaaat cgattcgcgt
      901 attgtcgata tcgaagtgaa aatcctcgac gagcataaac ggttcaccca agcgatacgc
      961 acactgacgg atgcacgcca aagcgccgcg aacgtgatga gccgtgaggt gacctcgtcg
     1021 ctcggcgcac tcggcatgga ccacgcgcga ttcgaagtac aattgacgcg gacgaaagcg
     1081 gattccgtcg atcccgtcga gtcggatgcc gttgaggtag acgggcaggt gtacgaggcg
     1141 agtccgactg gcgcggaaga ggtggagttt ctcatcgcgc cgaatgtcgg cgagccgctt
     1201 cgaccactcg ttcgcatcgc gtcgggcggg gaaatcagcc gtacgatgct cgcgatcaag
     1261 agcgtgatct cggagcacga tccggtgacc accatggtgt tcgacgaaat cgacgccggc
     1321 gtgagcgggc gcatcgcaca ggcggtcggc gaacgaatgc gtgaactcgc ccgaaaacgg
     1381 cagatcgtcg cgattacgca tctaccgcag atcgccgcgt tcgccgatga acacatcgtc
     1441 gtcagcaagc acgagcacgc cggacgcacc ataaccgaag cgaacgtcat cacgggcgaa
     1501 gcgcgcacaa acgctctcgc agcgctgttc ggcggagcga aggtatccga aaccgctctt
     1561 gaacacgcgc gcgcgttact cggagagcgt cgctgatgga tgccgagaat acgtttatac
     1621 ggagtctggt gcgtggtgtg cgtcagaagc taaaagtccc gcgcggacgc atattcagtg
     1681 cgggaaacct tgtgagcatg agtcgcgtag cgctgttacc gtttatgctg tcgctgctga
     1741 aaaaaccggc cgaggggaag tggctcgtgc tgtcgattgt cctcgcggcg atcatcgcgg
     1801 cgacggacgc actcgacgga ttcatcgcgc ggcagttgaa tcaggtgagc gaactgggac
     1861 gggtgttcga tcccattgcc gacaagattt gcatcgggtt cgttggggtg tggctctcga
     1921 cgtatcgcgg actgccggta tggattcccg cggtgattat cgcgcgcgac gtggtaatcg
     1981 tgatcggggc gattgtgatt gctcgcaaga ccgatatcgt catgccgtca catcaggtag
     2041 gtcgagtcac gacgttcgta ctgaccgtga cctttttcat ctacatcatc gactggcagt
     2101 ggccgcagat ggcactcgtg tggacaagcg gcgcgctaat cttcatgtcg ttcgccgtgt
     2161 atctgcggat cggatggcat atgctgcatc agcggacgtg gatcgcgtaa aaggtcacgc
     2221 ggatcgatgg ggaatgcggg cgcgccgcag aggaagagaa agttggcgta cgcgatgcaa
     2281 agcgatcctg cagaatacaa caggcaaccg aggtgagact gtgaatatac ctcagctttg
     2341 tcggacggag gttacaaatg gacatccgcc caaatggtat tcttttagta
//
LOCUS       DSCC01000281            2303 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031726, whole genome shotgun sequence.
ACCESSION   DSCC01000281 DSCC01000000
VERSION     DSCC01000281.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031726"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..688
                     /gene="hemH"
                     /locus_tag="ENN56_03470"
     CDS             <1..688
                     /gene="hemH"
                     /locus_tag="ENN56_03470"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: protein motif:HMM:PF00762.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDS74579.1"
                     /translation="EETLSELKRAGIRRLVALTLYPHYSIATTGSSINELQRVIERDF
                     ASDFEITYITEWPELPGYLDALAAKIESALKEVSEDRRDKVTLLFSAHGLPVDFIDRG
                     DPYIEHIETTIDGVMSRLERKPPWCLAYQSRVGPVKWLEPTTPDTMRRLAAKGVRDVV
                     IVPVSFVTDHIETLEEIDRQFGRLANELGYSTFHRTKALNDDPMFISALGNLVLDHLQ
                     SAGFEEVRNG"
     gene            600..2072
                     /gene="hemG"
                     /locus_tag="ENN56_03475"
     CDS             600..2072
                     /gene="hemG"
                     /locus_tag="ENN56_03475"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDS74580.1"
                     /translation="MTIRCSSRRSGISCLITCSQQVSRKCVMGEIAIIGGGIAGLATA
                     FEILERSPNARVRIYEASDRLGGNIRTDRVDGYTVEWGPNGFLDNVPETLDLIARLGI
                     SNRLKPASLASKRRFIWRNGGLHEIKANPVSFLASDLLSWQGKLRLMAEPFTGSPPSY
                     DQTVFEFASRHIGAEAARVLVDAMVSGVFAGDSRQLSLQSAFPKMHAMESQYTSLVRA
                     MLGRMRDRRAGRSSGKTGGPSGPAGHLTSFEGGMSELVDAVANAVGRKNIVLNTPVSW
                     IAFDRPYGFHIHLKNDEEIDVESVILAAPAWNAGPLVDGIAPGIAQTLRKIPRAPLAV
                     VALGWKREAFSYPLNGFGFLVPRGESLTILGSLWTSSVFPGRADENHVLMRTMIGGAH
                     CPMELQKTDEELLATVRADLGVVFGNLPEPEFERVYRFENGIPQYTVGHSERVRVVEE
                     ACARLPGLYVTGNSYHGISVNACVAEAGPLADRVLAALSG"
BASE COUNT          504 a          612 c          689 g          498 t
ORIGIN      
        1 cgaagagaca ctcagtgagt tgaagcgcgc aggcattcgc cgccttgttg cgctgacgtt
       61 gtatccccat tacagcatcg ctacgaccgg ttcgagtatc aacgaactgc agcgcgtaat
      121 cgaacgcgac ttcgccagtg acttcgagat aacgtacatc actgaatggc cggagttacc
      181 cggatacctc gatgctcttg cggcgaaaat cgaaagcgcg ctcaaagagg tttctgaaga
      241 tcggcgtgat aaggtgacgc tgcttttcag cgcgcacggg cttcccgtcg acttcatcga
      301 ccgtggagac ccatacattg aacacatcga aacgacgatt gacggtgtga tgagtcggct
      361 ggagagaaaa ccgccgtggt gcctcgcgta ccagagccgc gtcggaccgg tcaagtggct
      421 cgagccgaca acaccggata ccatgcgtcg attggctgcc aaaggtgtac gtgatgttgt
      481 gattgtgccg gtgtcgtttg ttacagatca catagaaacg ctcgaagaga tcgatcgtca
      541 gttcggaaga ctcgcgaacg aacttggata cagtacgttt catcgcacga aagcgttgaa
      601 tgacgatccg atgttcatct cggcgctcgg gaatctcgtg cttgatcact tgcagtcagc
      661 aggtttcgag gaagtgcgta atgggtgaga tcgcgatcat cggcggtggt atcgcggggt
      721 tggcaaccgc gtttgagatc ctcgaacggt cgccaaatgc gcgcgtgcga atctacgaag
      781 cgagcgaccg gctcggtggg aacatccgca ccgatcgcgt agacggatac actgtcgaat
      841 ggggtccaaa cggatttctc gacaatgtcc cggaaacact cgacctcatt gcgcgactcg
      901 gtatttcgaa tcgattaaaa ccggcttcgt tagcttcgaa gcgtcgattt atctggcgaa
      961 acggcggact gcacgaaatc aaagcgaatc cggtttcgtt tctcgcgtcc gatctcctat
     1021 cgtggcaggg caagcttcgg ctcatggctg agccgttcac cggatcgccg ccgagttatg
     1081 atcagacggt attcgaattc gcctcacgcc atatcggtgc tgaagcggca cgagtgctcg
     1141 tcgacgcgat ggtgagtggc gtctttgccg gcgattcacg tcaactcagc ctccaaagtg
     1201 ctttccccaa gatgcatgcg atggaatcgc aatacacgag tctcgttcgc gcgatgctcg
     1261 ggcgtatgcg cgatcgtcga gcgggcagga gttccgggaa gaccggtgga ccatccggac
     1321 cggcggggca tctgacgtcg ttcgaaggtg gaatgagtga actcgtcgac gcggtagcga
     1381 acgccgtcgg tcggaagaac atcgtgctga acacgcccgt gtcgtggatt gccttcgacc
     1441 ggccatatgg gttccacata cacctgaaaa acgatgaaga gatcgatgtt gaatcggtca
     1501 ttcttgccgc accggcatgg aatgcgggac cactcgtcga tgggatagcg ccggggattg
     1561 cgcaaacgct ccgcaagatt cctagagctc cgttggctgt cgtcgcgctt ggatggaaac
     1621 gggaagcgtt ctcgtatccg ttgaatgggt tcggttttct ggttcctcga ggcgagagtc
     1681 ttacgatact tggcagtctg tggacatcga gcgtctttcc cgggcgcgcg gatgagaatc
     1741 atgtgttgat gcggacgatg atcggtggcg cgcactgccc gatggagctt caaaagacag
     1801 atgaggaact tttagcgacg gtgcgagccg acttaggcgt cgtgttcggg aatctcccag
     1861 agccggaatt cgagcgtgtt taccgattcg aaaacggtat cccacagtac acggtcgggc
     1921 actcagaacg cgtgcgagtc gtcgaggagg cgtgtgcacg gcttccgggg ctgtatgtaa
     1981 cgggaaactc gtatcacggc atttcggtga atgcctgcgt tgccgaagcg ggaccgctcg
     2041 cagacagagt cctcgccgct ctgagcggtt agtaaatcat cgttcgtgcc gagccaactc
     2101 cgctgcgaac gcccggtacg cttcttcgcc atagagcacg aaacgaacca gtttcagcga
     2161 cactgcctca gagagaaacg ccacggccac cgagaccatg atttccgcgc acgcatctat
     2221 cctgaagtga cctacacttt ttgacggcag actcgctccg caagcgcttt tacatcgtag
     2281 tttggatagg tgtatccgaa tgc
//
LOCUS       DSCC01000282            1565 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053735, whole genome shotgun sequence.
ACCESSION   DSCC01000282 DSCC01000000
VERSION     DSCC01000282.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053735"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(388..1515)
                     /locus_tag="ENN56_03480"
     CDS             complement(388..1515)
                     /locus_tag="ENN56_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS74581.1"
                     /translation="MTDGVNPTEFTVNFTVIANEPPVWHVSMPTSVIGIEDQPIDQIG
                     VLAIDPEGKGVTYSLVSAPAWIEFDGMAITGTPGFADIGSALITVSASDGMMSVDHEI
                     DVTVNPYYGDATKDGSISALDASMVLQHKVKKVAEIDFHATNVTREKGTDPIAPSAWD
                     AAHILYKIVMPGYIFPVQGGETLSKPVRKTEATLAWVQSASDWILKASGGFGPVGTDL
                     VLELPQDATISSEASFAYERNGDLVTIGVAAVGSEAELLRISGLTGAPEVVSVMLNNT
                     PAMIAEPLSFQLRQNAPNPFNPSTTIAFTVPEAAPVTLSIYDANGRLVRTLLKGEREA
                     GFHEIVWDGLDNSGRAVASGMYLYRLTAGDNTAARKLLLVR"
BASE COUNT          351 a          455 c          392 g          367 t
ORIGIN      
        1 ccgcgtcagg cgtcagcgaa aaaaggcgaa cggtacctcg aggtaatcgt cgaacggctt
       61 gcgaagtttc tcagaaagct ttccgagacg ccatacggac tgaatatgcc gtattcacag
      121 caagtgataa attagtttca cattcgtgac cgctgtcaca gtccaggagt tcgcgaaagg
      181 ctattattag gtattgatac ggtttccgga agaacgcttc tcggagtgca gagacctctc
      241 aaattgtcgg gacgaaactc gtgcgtgccc aactcgtccc gcggtttttg agaggtctct
      301 tttttttaca acacactccg tacacaaatc gagggcgaac caatcacatc aggaccgccc
      361 tcgactctcg ttttcccgtc gttcacgtca tcgtacgagc agtaacttcc tcgcagcagt
      421 gttatcgccg gcggtgagtc gatacagata cataccactc gcaaccgctc gcccgctgtt
      481 atccagcccg tcccacacga tctcgtggaa accggcttcg cgctcgcctt tcaataatgt
      541 ccgcacaagt cggccgttcg cgtcgtagat cgacaacgtc accggagctg cttccggaac
      601 ggtaaacgca atcgttgtcg acggattgaa cgggtttggc gcattttgtc gtaattggaa
      661 tgaaagcggt tctgcaatca tcgcgggcgt attgttgagc atcaccgata ccacctcggg
      721 agcacccgta agtccggata tccgcagtag ctccgcctca ctcccgacag ccgcaacgcc
      781 gattgtcacg aggtcaccgt tgcgttcgta cgcgaacgac gcttcgctgg aaatcgtcgc
      841 atcttgtggc agttcgagca ccaaatccgt acctaccgga ccgaagccac ctgatgcttt
      901 gagtatccag tcgctcgctg actgtaccca cgcaagcgtc gcttcggttt tccgcacagg
      961 cttcgacaac gtctcgccgc cctgaacggg gaatatgtaa cccggcatga cgattttata
     1021 gagaatgtgc gcggcatccc aagccgaggg agcgatggga tcggttcctt tttctcgtgt
     1081 tacattcgta gcgtgaaaat caatttccgc aaccttcttc actttatgct ggagcaccat
     1141 tgatgcgtcc agcgcggaga tcgaaccgtc ttttgttgcg tcgccgtagt acgggttcac
     1201 cgtaacatcg atttcgtgat ctaccgacat cattccatca gaagcagaaa ccgtaatgag
     1261 agcactacca atatccgcga atccgggggt tccggtaatt gccataccgt cgaactcgat
     1321 ccatgctggt gccgagacaa gggagtacgt tacgcccttt ccctccggat cgatcgcaag
     1381 aacgccgatt tgatctatgg gttgatcttc aattccgatt accgaagtcg gcatcgacac
     1441 gtgccatacc ggtggttcgt tcgcgatcac cgtgaaattg accgtgaatt cggtcggatt
     1501 cacaccatcg gtcacagaaa gcaccaccga cgccggtcct acgtcccctg cacccggtgt
     1561 tccgg
//
LOCUS       DSCC01000283            1715 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047459, whole genome shotgun sequence.
ACCESSION   DSCC01000283 DSCC01000000
VERSION     DSCC01000283.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047459"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            55..1008
                     /locus_tag="ENN56_03485"
     CDS             55..1008
                     /locus_tag="ENN56_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74582.1"
                     /translation="MGERVFEKLADPKALKRSPIFIFAADALVAYGITGIGTERTPHP
                     HYRTRPEYLKMMREVAADGYIDGLLMTPADAEVLAIDEGLFDSSPITPIVRMNAETHI
                     WSPRHGRYREQYSRPFQTVKTAEAGRYCTEAMACARDCKIKLGLYSITLNNEAEADAR
                     TTGEFLEFAWQCGETPDFHYFLEVFLPNIQQPGMDTKEQGEYIADNIVRLMSYLRNHQ
                     RPVFIKTVYTKPEVWKSLCEFDPTLVIGALGGSRVNARTSMQLAYDTTENGGKVILFG
                     RAIFQEEDPRAMAKMMRAVLDRELTVDQAHSQYQAILRARA"
     gene            1113..1571
                     /locus_tag="ENN56_03490"
     CDS             1113..1571
                     /locus_tag="ENN56_03490"
                     /inference="COORDINATES: protein motif:HMM:PF13380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDS74583.1"
                     /translation="MSRNQQSMIEDFLALHRIAFIGVSRNPRDFSRLLDRELVTRGYD
                     VVPVNPQTQEIDGRRSFSDIAAIDPPVDGALIMLPPQRTADALNACADAEVARVWIHR
                     GCEPPNGHLAMRNSKLMVISGECMFMFLPNTGLIHRIHRAIDRFTGRYPI"
BASE COUNT          371 a          506 c          487 g          351 t
ORIGIN      
        1 accttttact caaacaaaat catcgcgtca tacggtacat acttggagag actcatgggc
       61 gaacgcgtat tcgaaaaact tgccgatcca aaggcgctca agcgctcgcc gattttcata
      121 ttcgctgcag atgcgttggt ggcatatgga attaccggca tcgggacgga gcgcactccg
      181 cacccgcact accgcacacg ccccgaatac ctgaagatga tgcgtgaggt cgcggccgat
      241 ggatacatcg acggactgct catgacgccg gcggacgcgg aagtgctcgc gatcgacgag
      301 gggctgttcg attcgtcgcc aatcacaccg attgtgcgca tgaatgcgga aacgcatatc
      361 tggagtcccc ggcacgggcg gtatcgggag cagtattcgc ggccgttcca gacggtgaaa
      421 accgcggaag cgggtcgcta ctgcaccgaa gcgatggcgt gcgcgcgcga ctgcaagatc
      481 aagctcggac tctactcgat cacactgaac aacgaggccg aagccgatgc gcgtaccacg
      541 ggcgagttcc tcgaattcgc atggcaatgt ggcgaaactc cggatttcca ctacttcctc
      601 gaagtgttcc tgccgaacat tcagcagccc ggcatggata cgaaggaaca gggcgagtac
      661 atcgccgaca acattgttcg ccttatgtcg tacctgcgga atcaccagcg gccggtgttt
      721 atcaagacgg tctataccaa gccggaagta tggaagtcac tctgcgaatt cgacccaacg
      781 ctcgtcatcg gagcgctcgg cggtagtcga gtgaatgcac gtacgtcgat gcagcttgcc
      841 tacgacacga cggagaatgg tgggaaggta atcctgttcg gtcgcgcgat cttccaggag
      901 gaagatcccc gcgcaatggc gaagatgatg cgcgcggtgc tcgaccgtga gctcaccgtc
      961 gatcaggcgc actcgcagta ccaggcgatt ctgcgcgcgc gggcgtagtg tgcgcacgaa
     1021 cgcagcggag agagcaaagc gctgacttct ccggtccgcg ttttgcatcg tgtaaacggt
     1081 gaaccttgtt tcccccggag aggttgaccg tcatgagccg aaatcagcag tccatgatcg
     1141 aagactttct cgcgctacat cggatcgcgt ttatcggcgt atcgcgaaat ccacgtgatt
     1201 tcagccgtct cctcgatcgt gagctcgtca cgcgtggcta cgacgtcgtt ccggtaaatc
     1261 cccaaaccca agaaatcgac ggtcgccgct cattctccga tattgccgcg atcgatccac
     1321 cggttgacgg cgcgctcatc atgcttccgc cccaacgcac tgccgatgcg ctcaacgcgt
     1381 gcgcggatgc cgaagtcgcg cgggtatgga tacaccgcgg ttgcgaacct ccgaacggcc
     1441 atcttgccat gcgaaacagc aagctcatgg tgatttccgg cgagtgtatg ttcatgttcc
     1501 tcccgaacac cgggctcatc catcgaattc accgcgcaat cgaccgattc accggacgct
     1561 atccgatttg ataccgaggt gacacgatgc gctcgttgat gtccactcgt tttacggtaa
     1621 tcgctgttac tttcgccata ctcgccgtaa acgccgtaaa ccctggtgat gtgtgggcgg
     1681 cgtcggccga tcgtgcattc cagtcgttta cgtcg
//
LOCUS       DSCC01000284            1998 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038550, whole genome shotgun sequence.
ACCESSION   DSCC01000284 DSCC01000000
VERSION     DSCC01000284.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038550"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..763
                     /locus_tag="ENN56_03495"
     CDS             <1..763
                     /locus_tag="ENN56_03495"
                     /inference="COORDINATES: protein motif:HMM:PF05592.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74584.1"
                     /translation="LWCLRECADLARALGSDDAAREWSAMYDHARSAFDGMFFVSDPK
                     LWRDNVAFDAAGRQNEPTYCESTIFTIIRTGLLDRERGLEAIDRLMNPTFLCCRTSGG
                     LEGSAYPKFFMDIGETDVALEYWLDRWGAPIAADATTCGEEFFQSAGNSDCHIHGAGP
                     ARDFIEHLAGIRIRGARWSDVLLVPPTDGALLPDLRATVPTPHGRIEVTIETVADGAR
                     QYRWALPDGACAKLLLRDEEISIETTGSVPLPEQP"
     gene            967..>1998
                     /locus_tag="ENN56_03500"
     CDS             967..>1998
                     /locus_tag="ENN56_03500"
                     /inference="COORDINATES: protein motif:HMM:PF00474.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74585.1"
                     /translation="MNGSGVQLRNLDILAIILYIVAMLALGWWATKRVKNTEGYFVGG
                     RKVPGWAVGISMLGTAISSVTFLAYPGSAFAGNWSRLVPGLMLPVATIIGAYFFVVFF
                     RRTMFVSAYQYFEHRFGSWGRSYASAMWSLASMYRMGSILYLMSLPIRLITGWDILTV
                     MIVFGLLITVYTMMGGLEAVIWTDVVQTVVLFLGGLITILIVFIKVPGGPLEVLAPAW
                     NAGKFGVAVDVDFTFIRDTFWVLALSGLVGNIQEYSTDQTKIQRYLAADSDKGALTAI
                     WTVGIGCIPLWSLFMFVGTCLWVYYAHHPEAIELARQTLGTDLRSDTVYPLFIITVLP
                     PFVGGLVISA"
BASE COUNT          361 a          590 c          583 g          464 t
ORIGIN      
        1 gctctggtgc ctccgtgagt gcgccgatct cgcccgtgct ctcggttcgg atgacgccgc
       61 gcgagaatgg tccgcgatgt acgatcatgc acgctccgcg ttcgacggca tgtttttcgt
      121 ttccgacccg aagctctggc gagacaatgt cgccttcgac gccgcagggc ggcaaaacga
      181 accgacgtac tgcgaatcga ccatcttcac cataattcgc accgggctgc tcgaccgtga
      241 gcgtggtctc gaggcgatcg atcgactcat gaaccccacg ttcctctgtt gtcgcacctc
      301 cggcggcctc gaaggtagcg cgtatccgaa gtttttcatg gatatcggcg agacggatgt
      361 agcgctggag tattggctcg accgttgggg cgcgccgatc gccgctgatg cgacgacatg
      421 cggtgaggag tttttccaat cagcggggaa tagcgattgt cacatccacg gcgccggacc
      481 tgcgcgcgat ttcatcgagc atctcgccgg tatccgtatt cgtggcgctc gttggtcgga
      541 cgttctcctc gttccaccga ctgatggtgc actgcttccc gatctacgcg ccacggtgcc
      601 gacgccgcac ggcaggatcg aagtcaccat cgagaccgtt gcagacggag cgcgccaata
      661 ccggtgggcg cttcccgacg gtgcgtgtgc gaagctacta ctgcgagacg aagaaatctc
      721 gatcgaaacc acgggctcgg taccgctacc agaacaacca taatcgcacc gttacctcga
      781 cggtgccagt tttccttttt cgaattctgc gtttgacaaa ccggcttggt cgcggaaaca
      841 catattacta ccttcattcc tcggtaatcc agcgcgaaca atccctcgca ccacaccaca
      901 gtagtatggc ggcaccgtgc gggaataccc cgatgagccg cgtcgtccgg aaggagaatt
      961 gtctcgatga atggatccgg tgtgcaactc cgtaacctcg acatattggc gatcatcttg
     1021 tatatcgtcg cgatgttggc actcggttgg tgggctacga agcgtgtcaa gaatactgag
     1081 gggtatttcg tcggcggacg gaaagttccg ggttgggcgg tcggaatttc gatgcttgga
     1141 acagcgatca gttccgtgac cttcctcgcg tatccgggtt cggcgttcgc aggtaactgg
     1201 agccgcctcg ttcccggtct catgctgccg gtagcgacca ttatcggcgc atacttcttc
     1261 gtggtgttct tccgacggac gatgttcgtc agcgcctacc aatacttcga gcaccgtttc
     1321 ggttcatggg gccgcagtta cgcgagcgcc atgtggagcc tcgcgtcgat gtatcgcatg
     1381 ggatcgattc tctatctcat gtcgctgccg attcgtctga tcaccggttg ggatattctc
     1441 acggtcatga tcgtcttcgg cctgctgatc accgtgtaca ccatgatggg cggcctcgaa
     1501 gcggtgatct ggaccgacgt cgtgcagacc gtcgtcctgt tcctcggcgg actgattacg
     1561 attctcatcg tgttcatcaa agttcccggt ggaccgctgg aggtgcttgc accggcgtgg
     1621 aacgcgggga agttcggtgt cgcggtggac gtcgacttta cctttatccg agataccttc
     1681 tgggtgctcg cgttgtcggg tctcgtgggg aatattcagg agtattcgac cgaccagacg
     1741 aagattcagc ggtatctcgc cgccgacagc gacaaaggcg cgctcaccgc gatctggacc
     1801 gtcggtatcg ggtgtatccc gctctggtcg ctattcatgt tcgtcggaac ctgtctctgg
     1861 gtctactacg cgcatcatcc ggaagcgatc gagctcgcgc ggcagacact cggaaccgat
     1921 ctgcgttccg acacggtata tccgctcttc atcatcaccg tgctcccgcc gttcgtcggc
     1981 ggactcgtaa tcagcgca
//
LOCUS       DSCC01000285            1525 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055556, whole genome shotgun sequence.
ACCESSION   DSCC01000285 DSCC01000000
VERSION     DSCC01000285.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055556"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(513..1484)
                     /locus_tag="ENN56_03505"
     CDS             complement(513..1484)
                     /locus_tag="ENN56_03505"
                     /inference="COORDINATES: protein motif:HMM:PF13229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HDS74586.1"
                     /translation="MLVSEHFLTGDMDAIDNTVISGSDSAFVVSIEGEMARLPVIHGF
                     TIRDGSDGITATVPFELYHSHITKTGDGIDYEGGGGLVRHCRFTQNRDDGIDLDYAVA
                     VRIEHCIISNNGDDGIEIRLQPFSMGPNPYEGDSLHTVMRCNLIEGNGENGIQFIGYD
                     EETARTFRIEGNFIIDNAMAGIGCMDGHNTREDYSAAPLLESIIVRNNTFIGNDHHLS
                     GGANLLAVNNIFVGAETLSVKNVSGNSLVTHNLFWDNRADAFGSNIDVARTLYVDPRL
                     LSDARPRRGSPVVDAGIEEVEWNGRRVRVLPPREYRGSAPDIGAWQR"
BASE COUNT          334 a          467 c          400 g          324 t
ORIGIN      
        1 acatagaaga gatgctcgaa gatgccgagt ccgacggatc atttgagtgt aacgatcgtg
       61 cgacgatcat tgcccggaca atggtgagtc tcgggttccg cggtcgacaa gtgccacttc
      121 agagtcgggc gggcgatggg cacatgctcg tcgaggcgtg gttgcccgac ctcgaaacgt
      181 ggatggcgta cgacccgatg tcggagcaat acttcctcat cgacggcaaa cccgcgtcgg
      241 cgctcaaact ccgtgactac gttcgcgccg gacgacgcaa tgccgtcgaa gtatcctccg
      301 gaccaccgtt cagcgagatc gcaaagtatt tcacacatgc gcggatggct ctgacggcgg
      361 acgaacgcgt gccgaatatc acgcccgcac aggcggtaca attccgaact gacgacgacc
      421 tgctcgtaca atttcgcaac gggcactggt acgaatggcc tattgaacag taaacgctca
      481 ccgcactcgc cattcgcgtc acgaaactca cactaccgct gccacgctcc gatatccggt
      541 gccgacccac ggtattcccg tggcggaaga actcgcactc tccgaccgtt ccactccact
      601 tcctcaatac cggcgtcgac gaccggacta cctctgcgcg gacgcgcgtc tgacagaagt
      661 cgcgggtcta catacagcgt cctcgccaca tcgatgttcg acccgaaggc gtccgctcgg
      721 ttatcccaaa agaggttgtg cgtcaccaat gagttaccgg agacgttctt cacgctgagt
      781 gtctcagcgc caacgaagat gttgtttact gcgagcagat tcgcaccgcc gctcaggtga
      841 tgatcgtttc cgataaacgt gttattcctc acgatgatcg attcgagcag cggagcggcg
      901 gaataatcct cccgcgtgtt atgaccgtcc atacaaccga tgccggccat cgcgttgtcg
      961 atgatgaagt tcccctcgat gcggaatgtc cgcgcagtct cttcgtcata cccaatgaat
     1021 tgaatgccgt tctcgccatt tccttcgatg agattgcacc gcatcaccgt atgcaacgag
     1081 tcgccttcat aggggtttgg ccccatggaa aacggctgca accgaatctc aataccgtcg
     1141 tcaccgttgt tcgaaatgat acagtgttcg atccttaccg cgaccgcgta atcgagatca
     1201 atcccatcgt cgcgattttg ggtgaaccgg cagtgtctca cgagaccgcc gccaccctcg
     1261 tagtcgatac cgtcgcctgt tttcgtaatg tgggaatggt acaactcgaa cggcacagtc
     1321 gcggtgatgc cgtcgctacc atctcggatc gtaaaaccgt gaatcaccgg tagccgcgcc
     1381 atttcgcctt cgatactcac cacaaacgcg ctgtcggagc cggaaatcac cgtgttgtcg
     1441 atcgcgtcca tatcgcccgt gaggaagtgc tcggatacga gcacgatcgg cttcccatga
     1501 attcgaatcg actcggtgta cactc
//
LOCUS       DSCC01000286            4250 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1013974, whole genome shotgun sequence.
ACCESSION   DSCC01000286 DSCC01000000
VERSION     DSCC01000286.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1013974"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(92..1693)
                     /locus_tag="ENN56_03510"
     CDS             complement(92..1693)
                     /locus_tag="ENN56_03510"
                     /inference="COORDINATES: protein motif:HMM:PF13450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74587.1"
                     /translation="MIVRITDLKLPLGSPTHYLGDEVARRLNLTADDFGPIRIRRRAL
                     DARRASYLQYVYTVEVDTPLTERAIASIAGVEPVRADSVAPVVPGSHEMRGQPVIVGA
                     GPAGLFAAWRLSEYGYQPILLERGKPVEDRRYDTAAFWKNGTLDPDSNVLFGEGGAGA
                     FSDGKLTSRIKDARREEVLGILAMCGAPENIVYDARPHVGTNRLPYILRELRTRLLAQ
                     GTEIRFGTRMTDLGDTNGMRYVDTNHYRIDSGVVVLAIGHSARDTYAMLAKRNIALEP
                     KPFAMGIRVQHSQELIDRAQYGRAAGTQGLEAGEYVLTCARTNVGRAVYSFCMCPGGT
                     VIPCASEPGTLCCNGMSGEHRSGPYANGAIVAEVSTRDFADDTPLGGIELQRVWERKA
                     FEATGGTYALPVMALDDFVRGEFRKRSRDELALRRFPRAEFVDLHQLLPETTTAAIRQ
                     ASKQFNRQIRGWIGSGGVAFGIETRTSAPVRILRNETGESISTPGLFPAGEGAGYAGG
                     IISAAVDGLRAAEAVIKKYARSHAS"
     gene            complement(1690..2229)
                     /locus_tag="ENN56_03515"
     CDS             complement(1690..2229)
                     /locus_tag="ENN56_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74588.1"
                     /translation="MAKKNELRDVFDLNNVERAILTHCANSVEDTVYLIDLSTMIADL
                     PRGLRGAWFDEKLLSPKQLAAHTQAKKDLDAAMRRLFREDLVDVGREDTYPEGFKRHH
                     RELIASMRNGRYWLARPQYRGKVPSLREVEDHVAAHTEWAYATNLRAVHRQLGGAPDF
                     IFLTDRGREFATLLKNGDT"
     gene            2373..4145
                     /locus_tag="ENN56_03520"
     CDS             2373..4145
                     /locus_tag="ENN56_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74589.1"
                     /translation="MSAIKTNEHSTTKATTQGYFTLIRRLNLLRQVADFRERHGWHTA
                     GALLDRTRQFFRARGTVPYHFDTPTVLSKYSSGELRDANVFFGPAHSGQIVVFLRRSD
                     TRRCVLLHVVDGEVSEYGDFPLDKRFGSNRRAAIVSLYGSRGTSVFDRYINPVEYSAD
                     GESISAQVNVVLNGDSSDAIPAQLVGVPNDDGYQWSITRVGVAHDWYPDTNPKLFLPG
                     YEPRRIVYGLSNTRFICAGHRSSGYGESSWAIWNDRWSGQNPLPLIKDVPEDRIGAMA
                     VSDRGEDRTVYSVAIGKLPDAHPLAAQTPALFLARNPGQVSAIPLEGIADIRNVMAIH
                     VSGPESGIFHATRQGPTEQLAVYQNGVPVGAPLDGIVFHGMAVEDPVESERVVRVHFV
                     VSDENGRRLLLVSATSTAWPETFAMMGVPLDDLRAQITEQETALEDYRRSQSGSSTDV
                     LGLDKSKGQLERTTVFDWWRHVSRKIEQVTGSSPKPYISRTQAMLVLNEKRNSDGTRL
                     VEPICAPLADIFHVNAEVSGGLAENDEVVRLRREILTYFEEHGLVVRASSITANPELA
                     AKGDPVLFVIHPAATDRSETLPTE"
BASE COUNT          915 a         1183 c         1214 g          938 t
ORIGIN      
        1 acgaaactgt atgcgggaca cctgcgaatg tgccgtccgc gactggtgcg cgcggtcatc
       61 gggtactcgg ctcgattgtg ccgggaacga tttaggatgc gtgcgatcgg gcgtattttt
      121 taataaccgc ctccgctgca cgtagcccat cgactgcggc acttataata ccaccggcat
      181 agcctgcacc ttccccggcg gggaagaggc cgggtgtcga tatcgactca ccggtttcgt
      241 ttcgtaagat gcgcaccggc gcactcgttc gagtctcgat gccgaacgcc acaccaccgg
      301 atcctatcca cccgcgtatc tgccggttga attgtttcga tgcttgccga attgccgcgg
      361 ttgtcgtctc gggaaggagc tgatggaggt caacgaactc ggcacgtgga aaacgacgaa
      421 gcgcgagctc gtccctgctg cgtttgcgaa attcgccacg gacgaaatcg tcgagtgcca
      481 tgactgggag cgcgtacgtc ccaccggtcg cttcaaatgc tttgcgttcc cacacacgct
      541 gaagctcgat tccgccgagc ggagtatcat cggcgaaatc gcgagtcgat acttcagcga
      601 cgatcgcgcc gttggcgtac ggcccggaac gatgttcacc gctcattcca ttacaacaaa
      661 gcgttccggg ctcagacgcg cacggaatga ctgttcctcc gggacacatg cagaacgagt
      721 agaccgcacg tccgacgttc gttcgcgcac aggtgagtac gtattcgccg gcttccagtc
      781 cttgagtacc ggcggcgcga ccgtattgcg ctcgatcgat cagctcctgc gaatgctgaa
      841 cgcgaatacc catcgcgaat ggtttcggtt cgagtgcaat attgcgtttc gcgagcattg
      901 catatgtgtc tcttgcgcta tgaccgatcg cgagtacgac cacaccggaa tcgattcgat
      961 agtggttcgt gtcgacgtaa cgcattccgt tcgtgtcgcc gaggtcggtc atgcgggtgc
     1021 cgaaacgaat ctccgtacct tgcgcgagaa gacgcgttcg aagctcgcgg aggatgtacg
     1081 gcagccgatt ggtaccgacg tgcggacggg cgtcgtaaac tatgttctcc ggtgcgccgc
     1141 acatcgcgag aattcccaga acctcttctc ttcgagcgtc tttgatgcgc gaggtcagtt
     1201 tcccatcgga gaatgctccg gcgcctcctt caccgaacag aacattcgag tcgggatcga
     1261 gcgtcccgtt cttccaaaat gccgcggtat cgtaccgtcg atcttcaaca ggtttgccac
     1321 gttctaagag gatcggttgg tatccgtact ccgatagccg ccacgccgcg aatagtccgg
     1381 ctggtcccgc tccgacaatg acgggttgcc cacgcatctc atgtgatccg ggcacaacag
     1441 gagctacgga gtccgctcgc accggttcga caccagcgat tgacgcgatc gcccgctcgg
     1501 taaggggtgt atcgacctcg acggtataga cgtattgcag atagcttgct ctgcgagcgt
     1561 ccaatgcccg gcgacggatg cgaatcggac cgaaatcgtc tgcggtaaga ttcagacgac
     1621 gagctacttc gtcgccgaga tagtgtgtcg gtgagcccag cggaagcttt agatcggtta
     1681 tacggacaat catgtatcac cgtttttgag gagtgtcgcg aactcgcgtc ctcggtccgt
     1741 gagaaatatg aaatccggag cacctccgag ttgacggtgt accgcacgaa gattggtcgc
     1801 gtacgcccat tcggtgtgcg cggcaacgtg gtcttcgact tctcggagcg atggtacctt
     1861 tccgcggtac tgcggtcgag cgagccagta ccgaccgtta cgcatggagg cgatcaattc
     1921 ccgatggtga cgcttgaatc cttccgggta ggtgtcttca cgaccgacat cgacgaggtc
     1981 ttctcgaaat aagcgccgca ttgcggcatc gagatctttt ttcgcttgcg tgtgtgccgc
     2041 taactgcttt ggtgaaagaa gtttttcgtc gaaccatgcg ccacgtaagc ctcgtggtaa
     2101 atcggcgatc atcgtcgata ggtcgattag gtatactgtg tcttcaaccg aattagcaca
     2161 gtgcgtaagt attgcgcgtt ctacattgtt aagatcaaat acgtcgcgta attcgttctt
     2221 cttcgccacg ccgtccgctc ccatcttttc aaccgtcgaa agtgcccgag cgatacgtta
     2281 tggaacctct cccacgttgc gtattggtcg gcaattcgcc attttcagcg ttcacaacag
     2341 tcgtaacgtg gctcactctc tcgaggtcga caatgagcgc catcaaaacg aatgaacatt
     2401 cgacaacaaa agcaaccacg caaggatact tcacgttgat ccgacggttg aacttgctcc
     2461 gtcaggtcgc cgattttcgc gaacgacacg gctggcatac cgcaggcgct ctcctcgatc
     2521 gaacgcgtca gtttttccgc gcgcgtggaa ccgttcccta tcatttcgac actcctacgg
     2581 tactgagcaa gtatagcagc ggagaactcc gtgacgcaaa cgtgtttttt ggtcccgcgc
     2641 actccgggca gattgtggtg ttcctccggc gtagtgacac tcgccggtgt gttttactgc
     2701 acgtggtaga cggtgaggtt agcgaatacg gagactttcc acttgacaag cgtttcggta
     2761 gcaaccgacg cgccgcgatt gtgagcttat acgggtcacg gggaacatca gttttcgatc
     2821 gatatatcaa cccggtcgag tattccgctg acggggaatc gatcagcgct caagtgaacg
     2881 ttgtccttaa cggagattca agcgatgcga tacccgcgca actcgtcggt gttccgaacg
     2941 acgatgggta ccagtggagc atcacacgag tcggagtcgc acacgattgg tatccggaca
     3001 ccaatccaaa actgtttctc cccgggtacg aaccgcgaag gatcgtttac ggcttgagca
     3061 atacgcggtt catttgtgcc gggcaccgaa gctccggata cggtgaatca tcgtgggcta
     3121 tatggaatga tcgatggagt ggccaaaacc cactgccact tatcaaagac gtacccgaag
     3181 atcgtatcgg agcgatggcc gtgagtgacc gcggcgaaga tcgaacggtg tacagtgtgg
     3241 ctatcggcaa acttccggac gcgcacccgc tcgcggcgca aaccccggcg ttgttcctcg
     3301 cacgaaaccc gggacaagtc tcggcgattc cgctcgaagg catcgccgac atacggaacg
     3361 tcatggcgat acacgtctcc ggtccggaaa gcgggatatt ccacgcgacg cgccaaggtc
     3421 cgaccgagca gctcgcggtg tatcagaacg gcgtgcccgt gggagcgcca cttgatggta
     3481 tcgtttttca cggaatggcg gtggaggatc ccgtagaaag cgaacgggtc gtccgggtac
     3541 acttcgtcgt ttccgacgaa aatggacgga gactgcttct cgtatcggcg acgagtaccg
     3601 catggcctga aacgtttgcg atgatgggag tgccgctcga cgatttacgt gcgcagatca
     3661 cggagcagga aactgcgctt gaggattacc gacggagtca aagtggatct tcgacggatg
     3721 ttttaggact cgataagagc aaagggcaac tcgagcgaac gaccgtattc gattggtggc
     3781 gacacgtgtc tcgcaagatc gaacaagtaa ccggttcatc gccgaaaccg tatatcagcc
     3841 ggactcaggc gatgctggtt ctcaacgaaa aacggaactc cgacggcaca cgtcttgtcg
     3901 agccgatttg cgcgccactt gcggatatct tccatgtcaa cgcagaggtc tcaggcggac
     3961 tggctgagaa cgacgaggtt gttcggctcc gtcgcgagat actcacgtat ttcgaggaac
     4021 acggactggt cgttcgcgca agtagtatca ccgcaaatcc ggaactcgcc gcgaagggcg
     4081 atccagtgct gtttgtcata catcccgctg cgacggatcg tagcgaaacg ttgccgaccg
     4141 agtgaacggt aatcggttga agtatctcct attgagtgat cagtttgata cggtagcccg
     4201 aaggaattac cgtctcaaga tccacctggt tcatgatcgc aaggtcgagc
//
LOCUS       DSCC01000287            1608 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1051857, whole genome shotgun sequence.
ACCESSION   DSCC01000287 DSCC01000000
VERSION     DSCC01000287.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1051857"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..404)
                     /locus_tag="ENN56_03525"
     CDS             complement(<1..404)
                     /locus_tag="ENN56_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDS74590.1"
                     /translation="MALLGLDIGTTGVKALVIDDAGHTLGAYTDEYPLSTPRPGWAEQ
                     DPEQWWRATITATKAVLAKAGVRGEQIHGVGLSGQMHSSVFLDEHDEVIRPALLWCDG
                     RTTEQRHRIVDTVGGEDSMRQLVSNPPLEGFTL"
     gene            696..>1608
                     /locus_tag="ENN56_03530"
     CDS             696..>1608
                     /locus_tag="ENN56_03530"
                     /inference="COORDINATES: protein motif:HMM:PF00400.30"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HDS74591.1"
                     /translation="MQFGSLLNSAPYTISLMVTNTISSNTHVKRNCLETPEQSSSGLG
                     LCHKYRPERSPHMNTSIFRLAAVTLIATFAITAQSFAQNVTFTGHSDVVKSVTISADG
                     KFAVTGSFDKTARMWNLESGMPVGVISEYPEAVLSVAAASNGHHLITGTADNRVMVCR
                     CTDGLPVKNFRGHTGPVYSVDITTDMRYVASTGIDRSVRVWNFAGDSLAMAFNAHRAP
                     VWTVKFSPDGSLLASAGSDRIVQLWSVSDTTHVRSFEGHTAPIWDVAFSADGSKLVTG
                     SVDKTVRIWDVATGNLVATMEGHTGSVY"
BASE COUNT          325 a          453 c          492 g          338 t
ORIGIN      
        1 agcgtgaacc cttcgagtgg tggattcgac acgagttgcc gcatcgagtc ttcgccgccg
       61 acggtatcca cgatccggtg tcgttgctcc gtggtgcggc cgtcgcacca gagtagcgcg
      121 ggacggatca cctcgtcgtg ttcgtcgagg aacaccgagc tgtgcatctg cccggaaaga
      181 cctacgccgt ggatttgctc gcctcgaaca cccgcctttg cgagaaccgc tttcgttgcg
      241 gtgatcgtcg cgcgccacca ttgctcggga tcctgttccg cccaacccgg acgtggtgtg
      301 gagagtgggt actcgtcggt gtatgcgccg agcgtatgcc ccgcgtcatc gatcacgagc
      361 gccttaacgc cggtggtgcc gatgtcgagt cccaacagtg ccatagcaag ttccctcgcg
      421 ggtggtgctg cgatcggtga tgataacgaa gcgcgcaaga tagtgtaagg tacgttccgt
      481 gcatttcggg tgtcaacgga tttcgacgaa tacgtatggt aggacagcga cgatattgtg
      541 cgaggtgaga tcggaatgcc cgctgatcgg ccattcgaag cgttttcgaa cgacaaatgt
      601 aacaattccg agaacgtgcc gtctgatcag gtgaccgaag agacagtgag caatagcccg
      661 ttgccgatgt gatatcggac gaggaggaat acacgatgca gtttgggagc ttgcttaact
      721 ccgcgccata caccatatct ttaatggtga cgaacaccat atcgtcaaac acccacgtga
      781 agaggaactg cctggagacc ccggagcagt cctcatccgg attagggtta tgccacaagt
      841 atcgccctga aaggagtccc cacatgaata cctccatctt tcgcctcgcg gcagtgacgc
      901 tcatcgccac attcgccatc accgcgcaga gtttcgcgca aaacgtgacc ttcaccggac
      961 actccgacgt cgtcaagtcc gtgacgatca gcgccgatgg caagtttgcc gtcacgggaa
     1021 gcttcgacaa gactgctcgg atgtggaatc ttgagtccgg aatgccggtc ggcgtgatct
     1081 cggagtatcc ggaggcggtg ctcagcgttg ctgcggcgtc gaacggccat catctgatta
     1141 ccggtactgc agataaccgc gtcatggtat gtcgatgcac cgacggtctt ccggtgaaga
     1201 acttccgcgg gcacactggt ccggtgtatt ccgtggatat tacgacagac atgcgatatg
     1261 tggcgtcgac cggtattgac cgcagtgttc gcgtctggaa cttcgccggt gattcgctcg
     1321 ccatggcgtt caacgcgcat cgtgcgccgg tctggacggt gaaattcagc ccggacggct
     1381 cgctcctcgc ttcggcgggt agtgatcgga tcgtgcaact gtggagcgtc tctgatacga
     1441 cgcacgttcg cagcttcgaa ggccatacgg cgccgatctg ggacgtggcg ttcagcgccg
     1501 acggcagcaa gctcgtcacg ggaagcgttg acaagaccgt gcggatttgg gacgtcgcga
     1561 cgggcaacct cgtggcgacc atggaaggcc atacgggtag cgtttacg
//
LOCUS       DSCC01000288            2146 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034981, whole genome shotgun sequence.
ACCESSION   DSCC01000288 DSCC01000000
VERSION     DSCC01000288.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034981"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..528)
                     /locus_tag="ENN56_03535"
     CDS             complement(<1..528)
                     /locus_tag="ENN56_03535"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS74592.1"
                     /translation="MTSHAQDIDTSEGFHTFTGCDTPIVEVCDLVHQRGDHTLVDGVS
                     FSVARGEYIALMGPNGSGKTTLARHLNAMLHPTSGVVCVLGKTTTDPSNTDAIRREVG
                     LVFQDPDDQMVAVTVEDEIAFGLENRAVPRNEMLRRVDETIARFELEHVRDRATTSLS
                     GGEQQRVAIAAGERCG"
     gene            558..863
                     /locus_tag="ENN56_03540"
     CDS             558..863
                     /locus_tag="ENN56_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HDS74593.1"
                     /translation="MDTSGGEQGKIEINLGENVSGTYSNFVVITHTGSEFVLDFSVMM
                     PGMQKAQVVSRVVLTPEHTKRLLAALGQNVQRYEAKFGTITLPIPPGGQGQQGGGNA"
     gene            complement(867..1400)
                     /locus_tag="ENN56_03545"
     CDS             complement(867..1400)
                     /locus_tag="ENN56_03545"
                     /inference="COORDINATES: protein motif:HMM:PF04892.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74594.1"
                     /translation="MPDSTAAEKPPTVARGKPVVPVNARRIVVVVWLAYSVAVLGISI
                     LPSEAVPKFLMFGWDKIANAIAYLASLLADDTVYTYPIIGWDKIAHASAFLGLGALTV
                     PVMLHRKQWITLTVGYGLAIAVLSEWLQSYAPGRTPSTADVAADLIGTGTAVIVSILW
                     LRLFGRAFASHGKQTRA"
     gene            complement(1393..>2146)
                     /locus_tag="ENN56_03550"
     CDS             complement(1393..>2146)
                     /locus_tag="ENN56_03550"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:PRK05385.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDS74595.1"
                     /translation="GARPIFFQDYIALGKHRGDVVANVVAGLAKACRATGCALLGGET
                     AEMPDMYAPGEYDLAGTIVGIVDRKRILDGSRVRVGDVLIGVGSDGLHTNGYSLARRL
                     IFDEARWSVDHFVPEWNRTVADELLRPHRPYLNAISPLLDDVLIHAMAHITGGGITDN
                     VPRVLPKGTAAKVYRDTWDIPPVFNTLYRLGNLDEAEALRTFNMGVGMVLICDSDDSE
                     TVMGRLRSSGERVWQIGEVVDGKDADGEVRYA"
BASE COUNT          460 a          647 c          607 g          432 t
ORIGIN      
        1 accacatcgc tctccggcgg cgatcgcgac acgctgttgc tcgccgccgg agagcgatgt
       61 ggtcgctcga tctcgcacat gctccaattc gaaacgcgcg atggtttcat cgacgcgacg
      121 gagcatttcg ttccgaggga cggcacggtt ttcaaggccg aacgcgatct cgtcttcgac
      181 ggtaaccgca accatttgat cgtcgggatc ctggaacacg agcccgactt ctcgccgaat
      241 cgcgtcggtg ttgcttggat ccgtcgtggt cttgccgagg acgcacacga caccggaggt
      301 cgggtgtagc atcgcgttca ggtgtcgcgc gagcgttgtt ttgccggaac catttggtcc
      361 catcaacgcg atgtactcac cgcgcgctac gctgaacgaa actccgtcca cgagtgtatg
      421 gtcacctcgt tggtggacga ggtcgcagac ttcgacgatt ggcgtatcac accccgtaaa
      481 cgtatggaac ccttcagaag tgtcgatatc ttgcgcgtgc gaggtcatgg acgtaccata
      541 tctatatgat aggacctatg gataccagtg gcggtgaaca aggcaagatc gagatcaatc
      601 tcggcgaaaa cgtttccggc acgtactcga atttcgtggt gatcactcat acgggcagcg
      661 agtttgtcct cgacttttcc gtgatgatgc cgggaatgca aaaggcgcag gttgtatccc
      721 gcgttgtgct tacaccggaa catacgaagc gacttctcgc tgcgcttggt cagaacgttc
      781 agcgctacga agcgaaattc ggtaccatta ccctcccaat acctcccggt ggtcagggac
      841 aacaaggcgg cggaaacgcc tgaccgtcac gcacgcgttt gcttcccgtg tgatgcgaac
      901 gcccgtccga acagcctgag ccataagatc gatacgatca ctgcggtacc cgttccgata
      961 aggtcggcag ctacgtcggc tgtgctcggt gtgcgcccgg gagcatacga ctgtagccac
     1021 tccgagagta cggcaatggc gaggccgtag cccactgtca gcgttatcca ttgtttacgg
     1081 tggagcatca cgggaaccgt gagcgcgcct aatcccaaaa acgccgacgc atgcgctatc
     1141 ttatcccagc cgatgatcgg atacgtgtaa acagtatcgt cggcgagcag cgatgccagg
     1201 tatgcgatgg cgttcgcgat tttgtcccaa ccgaacatga gaaacttcgg tacggcttca
     1261 ctcggaagga tcgagattcc gaggactgcg acagaatacg ccaaccaaac tacgacgacg
     1321 atgcgacgag cgttcaccgg cactacgggc ttgcctcgag ccaccgtagg tggcttctcc
     1381 gctgccgttg aatcaggcat accggacttc cccgtcggcg tccttcccgt ccacaacctc
     1441 gccaatctgc catacacgct cgcccgaact gcgtaggcgg cccatgaccg tctccgagtc
     1501 gtcggagtca cagattagca ccattccgac gcccatgttg aacgttcgca gcgcctcggc
     1561 ttcatcgaga tttcccagtc ggtagagcgt attgaatacc ggcggtatgt cccacgtgtc
     1621 acgatacacc ttcgcagcgg ttccctttgg aagcacgcgc ggaacgttat ccgtgatgcc
     1681 gccgcccgtg atatgcgcca tggcgtggat gagcacgtca tccaaaagcg gagaaatcgc
     1741 gttgagatag gggcggtgcg gacgaaggag ctcgtcggca acggtgcgat tccattcggg
     1801 gacgaaatga tcaacgctcc aacgagcttc gtcgaaaatc agacgacgtg cgagcgagta
     1861 cccgttcgta tgaagaccgt ccgaaccgac tccgataagt acatctccta cgcgtacgcg
     1921 gctaccgtcg aggattcgct tacgatcgac gataccaacg atcgtaccgg cgaggtcgta
     1981 ttcccccggc gcgtacatgt ccggcatctc cgccgtctca ccaccaagaa gcgcgcaacc
     2041 cgttgcgcga catgccttcg ccaaaccggc gacgacattc gcgacgacat cgccgcgatg
     2101 cttgccgagc gcgatatagt cctggaagaa gatcgggcgc gcgccc
//
LOCUS       DSCC01000289            2038 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037535, whole genome shotgun sequence.
ACCESSION   DSCC01000289 DSCC01000000
VERSION     DSCC01000289.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037535"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="ENN56_03555"
     CDS             complement(<1..1397)
                     /locus_tag="ENN56_03555"
                     /inference="COORDINATES: protein motif:HMM:PF00171.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDS74596.1"
                     /translation="MKIDEAQVRAIVEQVVKSIGSTNGAKDHPSSTSTDGSGVKPAPV
                     GIGNGVFRTIGECIAATKAAQKEWIALPIEKRKAAVEAIRRAGVENAEMFARQTVAET
                     NIGRVADKIIKHHLASLKTPGFEDLDTKAWSGDGGLTIEEMAPYGVVGAVTPLTHPVP
                     TMINNSITNLVAGNGIVFNGHPGAKRIFAQAVQIVNQYIVKAGGPQNIISTTPEPTIE
                     SANELFSHPDVRMLLVTGGGGVVKAAMGSGKKVIAAGPGNPPVVVDETADIAKSAKDI
                     VDGASFDCNVLCTAEKEVFVVDLVADELKRRMVENGAYELTTTQIEKLADYAFSGDAG
                     CAHPVLNRAAVGKDAETLAQQIGITVPSTTRLLIGETPNENAFVQHEQMMSFVPIVRC
                     SDVNQAIDRAVAAEHGFGHTFIMHSTNLDNLDRMARLCNATVFVKNGPSGAGIGNGGE
                     GTLSFSIAGPTGEGIT"
     gene            complement(1475..1771)
                     /locus_tag="ENN56_03560"
     CDS             complement(1475..1771)
                     /locus_tag="ENN56_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutN"
                     /protein_id="HDS74597.1"
                     /translation="MILGRVVGSVVATQKDEKLRSTKLLVVETLDPLTGQGKKSYVVA
                     VDAVGAGDGEVVLCASGSSARLTPVTDGRPVDTVIMAIVDTYDIDGTVMFRKNE"
     gene            complement(1777..>2038)
                     /locus_tag="ENN56_03565"
     CDS             complement(1777..>2038)
                     /locus_tag="ENN56_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDS74598.1"
                     /translation="GMIETRGLVAAIEAADAAVKAANVTLVGAERVGGGLVTVLFRGD
                     VAAVKAAVDAGASAAQRVGEMLSVHVIPRPHESIDKIAPKAR"
BASE COUNT          368 a          617 c          561 g          492 t
ORIGIN      
        1 gtgatgcctt cgcccgtcgg ccccgcaatt gaaaaactga gtgttccttc accgccgttg
       61 ccaataccag ctcccgatgg tccgtttttc acgaacaccg ttgcgttaca gagtcgtgcc
      121 atgcggtcga ggttgtcgag gttcgtcgag tgcatgatga acgtgtgtcc gaacccgtgt
      181 tccgctgcga cggcacggtc aatcgcttga ttgacatcgg agcaacgcac aatgggaacg
      241 aacgacatca tctgttcgtg ctgcacgaac gcgttctcat ttggcgtttc gccgataagc
      301 aaccgcgtcg tagacggcac ggtaatgccg atctgttgtg cgagcgtttc ggcgtctttc
      361 ccgaccgcag cgcgattcaa taccggatgc gcacatcctg catcgccact gaaggcgtag
      421 tcggcaagtt tttcgatctg cgtcgtagta agctcatacg cgccgttctc gaccatacga
      481 cgcttgagtt cgtcggccac gagatcaacg acgaatactt ccttttcggc ggtgcagagc
      541 acgttgcagt cgaaggaagc gccgtcgacg atgtctttcg ctgatttcgc gatatccgcg
      601 gtttcgtcta caacgaccgg cggatttccc ggtcccgctg cgataacctt cttccccgat
      661 cccatcgctg ctttcacgac tccgccgccg ccggttacca gtaacatacg gacgtcggga
      721 tgcgagaaga gctcgtttgc gctttcgatc gtcggttcgg gtgtcgtcga tataatgttc
      781 tgcggtccac cggctttcac gatgtattga tttacgatct gaacggcctg tgcgaatatc
      841 cgctttgcac ccggatgacc gttaaaaacg ataccgtttc cggcgacgag gttagtaatc
      901 gaattattga tcatcgtcgg caccgggtgt gtcagcggag tgactgctcc gacaacgccg
      961 tagggcgcca tttcttcgat ggtgagaccg ccgtcgccgg accatgcttt cgtgtccaga
     1021 tcctcgaatc ccggggtctt caatgaagcg agatggtgct tgatgatctt gtcggcgacg
     1081 cgaccgatgt tcgtctcggc gacggtctgg cgcgcgaaca tttccgcgtt ttccacaccc
     1141 gcacgacgaa tcgcttccac ggccgctttt cgcttttcaa tcggaagcgc gatccattcc
     1201 ttctgcgctg ctttcgtagc ggcgatacat tcgccgatcg ttcggaatac accgttgccg
     1261 ataccgactg gtgcgggctt tactcccgaa ccatccgtcg atgtcgaaga cggatgatct
     1321 ttcgcgccat ttgtcgagcc gatggacttc actacctgct cgacgatcgc acggacctgt
     1381 gcttcgtcaa tcttcacgat gccgccctcc tgcgggctgg tcgtctacgc atcacgcaga
     1441 ttcgcgtgtg ctgcatcacg ccaattcgcg tgtgctattc gtttttgcga aacattacgg
     1501 tgccgtcgat atcgtacgta tcgacgattg ccataatcac ggtgtcgacg ggacggccgt
     1561 ccgtcactgg tgtgaggcgc gccgagctgc cgcttgcgca cagcacgact tcaccatcgc
     1621 ccgcgccaac cgcgtcaacg gcgacgacgt agcttttctt gccttgaccg gtgagcgggt
     1681 cgagtgtttc gacgacgagt aactttgtag agcgaagttt ttcgtctttt tgcgtcgcga
     1741 cgacactccc gacgacacgt ccgagtatca tgggtattac cgcgctttcg gggcgatctt
     1801 gtcgatgctc tcgtgtggac gcggaattac gtgcacgctg agcatttcac cgacgcgctg
     1861 cgccgctgat gcaccggcgt cgaccgcggc cttcacagca gcgacatcgc cgcggaacag
     1921 tacggttacg agtccgccac cgaccctttc cgcgccgaca agtgtaacat ttgccgcctt
     1981 gaccgctgcg tcggcggctt caatcgcggc cacgagaccg cgcgtttcga tcattcca
//
LOCUS       DSCC01000290            3238 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020183, whole genome shotgun sequence.
ACCESSION   DSCC01000290 DSCC01000000
VERSION     DSCC01000290.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020183"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            97..1452
                     /locus_tag="ENN56_03570"
     CDS             97..1452
                     /locus_tag="ENN56_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDS74599.1"
                     /translation="MFESLSSQLDDVFRRLRGRGTISEGNIKDAMGEVRRALLQADVN
                     FRVAKNFVKSVEEKAIGQSVVRSISPGQQIVKIVHDELVELMGGGAAQLELDGDPAVI
                     ALVGLQGSGKTTASAKLANQLKKRGRHPHLVAADIYRPAAIEQLMQLGESIGVPVFRA
                     PDGTDPVDIAKQAVKAARKAGDDVVIIDTAGRLNIDEELMRELERIRAAVSPNEILFV
                     ADAMTGQVAADVAKDFGDRLDFTGIILTKMDGDARGGAALSIREVSSKPIKFVSNGEK
                     LEDLDVFHPERMASRILGMGDVVSLVERAQETINREEAERLEARLRNKQFTLDDFLSQ
                     LQQVKKMGSLEQIIGMLPGVNSKMLDGALDDKALKHVEAIVHSMTKVERAHPDIINGS
                     RRRRIAGGSGRSIQEVNRLLKQFDVMRKMLTRGKPTQKSGAQQQPRRAKHFGKRRKSL
                     F"
     gene            1493..1876
                     /gene="rpsP"
                     /locus_tag="ENN56_03575"
     CDS             1493..1876
                     /gene="rpsP"
                     /locus_tag="ENN56_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDS74600.1"
                     /translation="MATAIRMKRIGAKKAPVYRIIVTDDRNKRDGRAIEVLGMYDPCT
                     EPASIELNNERVKHWLGTGATPSETVRNIFRKVGFYSPEGPSVERTKDLQRAPSVIAG
                     EKTHAEKVQEVVIETEALPKSDEEE"
     gene            1889..2404
                     /gene="rimM"
                     /locus_tag="ENN56_03580"
     CDS             1889..2404
                     /gene="rimM"
                     /locus_tag="ENN56_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDS74601.1"
                     /translation="MRPERAVIGRVLRPHGVRGEILVLPTTDVANRFRGLTTVWFVRD
                     GEELGKYEVESTRAMTKGVGLKLRRIDDRDIAAKFRGADVTAPALSPSELADREYFVS
                     DLVGLVAKNDDGEVLGTVAEVIDNPAHAVLVIDSDGKQWQVPLVSEFVSNVDIEGGVV
                     ALRLIEGLRDL"
     gene            2421..3122
                     /gene="trmD"
                     /locus_tag="ENN56_03585"
     CDS             2421..3122
                     /gene="trmD"
                     /locus_tag="ENN56_03585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDS74602.1"
                     /translation="MRIDVITLFPELFTSPLATSIVGRARDAGLADIEAHDLRRWASD
                     ERGTVDDAPYGGGGGMVLLPEPLTNAITELKAARTEPVYTVYLAPDGERLTQSLVNEL
                     ALEQRLILVAGHYRGIDERVRTTMIDREVSIGDYVLSGGELPALVLIDAVVRLLPGAL
                     GNFESALEDSFQDDLLDCPWYTRPREFAGMAVPDVLLSGDREAIRRWRREQALLRTRE
                     RRPDLLARRNGSARD"
BASE COUNT          696 a          841 c         1064 g          637 t
ORIGIN      
        1 gacgggcgtt gcgcgaacca cctcagtctt tgcatgtacg cggatatcgt gctatcttca
       61 agagtctcat tcttaacgga gtaagcggag caattaatgt tcgaaagcct cagctcacag
      121 cttgacgacg tattccgccg actacgcggt cgtggcacca tctccgaagg caatatcaaa
      181 gacgccatgg gagaggtgcg acgtgcgctg ctccaagcgg atgtaaattt ccgcgtcgcg
      241 aagaacttcg tcaagtcggt ggaagaaaag gcgatcgggc aatcggtcgt tcgcagtatc
      301 agccccggcc agcagatcgt gaaaatcgtt cacgacgagt tggttgagct gatgggcgga
      361 ggcgcggcgc agctcgagct cgatggcgat ccggcggtga tcgcgctggt tggcttgcag
      421 ggttccggca agacgacggc ttcggcgaag cttgcgaatc agctcaagaa gcgcggtcgc
      481 catccgcatc tcgttgcggc cgatatttat cgtcctgcgg cgattgagca gctgatgcag
      541 ctcggcgagt cgatcggtgt tccggtgttt cgtgcgccgg acggcaccga tcccgtcgat
      601 atcgcgaaac aagcggtgaa agcggcacgg aaggccggcg acgatgtcgt cattatcgac
      661 accgcaggtc gactgaatat cgacgaagaa ctgatgcgcg aactcgagcg gattcgcgcc
      721 gcggtatctc cgaacgaaat actcttcgtc gccgacgcga tgacgggtca ggtcgccgcc
      781 gatgtggcaa aggacttcgg cgatcggctc gatttcaccg gcatcatcct gacgaagatg
      841 gatggtgacg cgcgtggtgg cgcggcgctt agtattcgcg aagtgtcgag caagccgatc
      901 aagtttgtct cgaacggcga aaagctcgaa gatctcgacg tgttccatcc ggaacggatg
      961 gcgtcgcgaa ttctcggcat gggcgatgtt gtgtcgctcg tagagcgcgc gcaggaaacc
     1021 atcaatcgcg aagaggcgga gcgtctcgaa gcgcggctgc ggaataagca gttcacgctc
     1081 gatgacttcc tcagccagct tcagcaggtc aagaaaatgg gttcgctcga gcagatcatt
     1141 gggatgttgc ccggtgtgaa ctcgaagatg ctcgacggcg cgctggacga taaggcgttg
     1201 aagcacgtcg aagcgattgt gcactccatg acaaaagtgg agcgcgcgca cccggatatt
     1261 atcaatggca gtcggcgccg gcgcattgca ggcgggtccg gccgatcgat tcaagaagta
     1321 aaccgcctgc tcaaacagtt tgacgtaatg cggaaaatgc tcacgcgcgg caaaccgact
     1381 cagaagtcgg gcgcacagca acagccacgc cgagcgaagc atttcgggaa acggcgcaag
     1441 agcctgtttt agtgggatgt ggcgtagtta cgtcgtcccg ggaggaacac gcgtggcaac
     1501 agccatacgc atgaagcgga tcggagccaa gaaggcgccg gtataccgca tcatcgtgac
     1561 cgatgatcga aacaagcgcg acggccgagc gattgaagtg ctcggtatgt acgatccgtg
     1621 cacggagccg gcttcgatcg agttgaataa cgagcgggtg aagcattggc tcggaaccgg
     1681 tgcgacgccg agtgaaacgg ttcgcaacat tttccggaaa gtcggcttct attcaccgga
     1741 aggtccgtcg gtcgaacgga cgaaggatct tcagcgcgcg ccatcggtta tcgccggtga
     1801 gaagacgcac gccgagaagg tgcaggaagt cgtcatcgag accgaggcac tgccgaagtc
     1861 cgatgaagaa gagtaacgga gctcattcat gcgtcccgaa cgcgcagtga ttggtcgcgt
     1921 actgcgtcct cacggtgtcc ggggtgagat actcgtactt ccgacgacgg atgttgcgaa
     1981 tcgttttcgc ggattgacga ctgtttggtt tgttcgtgac ggcgaagagc tcggaaagta
     2041 cgaagttgag agcacgcgcg cgatgacaaa aggcgtcggg ctcaagctcc ggcgcattga
     2101 tgatcgtgat atcgcggcga agttccgtgg tgcggacgtc accgcgccgg cgttgagtcc
     2161 gagcgaactc gcggaccggg agtatttcgt ctccgacctt gtcggtcttg tcgcgaagaa
     2221 cgacgatggt gaggtgctcg ggactgttgc cgaggtaatc gacaatccgg cgcatgccgt
     2281 gctggtgatc gattcggacg gcaaacagtg gcaagtacca ctcgtatccg agttcgtgag
     2341 taacgtggat atcgaaggcg gtgtcgttgc gttgcggctg attgaagggc tgcgtgacct
     2401 ctgatcgcgg cggattcgcc atgcgcatcg acgtaatcac gttgtttccc gaattattca
     2461 cgtcgccgct tgcaacgagt atcgtcggaa gagcgcgtga cgcgggcctt gccgatatag
     2521 aagcacacga tcttcggcga tgggcatcgg acgagcgtgg tacggtggat gacgcgccgt
     2581 acggtggcgg tggcgggatg gtattgctgc cggagccgct gacaaacgcg ataacggagt
     2641 tgaaagcagc gcggaccgaa ccggtttaca cggtgtatct tgcgccggac ggtgagcggc
     2701 tcacgcagtc gctcgtcaac gaattggcgc ttgaacagcg gctgattctc gtcgcgggtc
     2761 actatcgcgg tatcgacgag cgtgtgcgca caacgatgat cgaccgggaa gtatcgatcg
     2821 gtgattacgt gttgagcggt ggagaactcc cggcgctcgt gctcattgac gcggtcgttc
     2881 ggttgcttcc gggtgcgctc ggtaacttcg agtcggcgct ggaagattcg tttcaagacg
     2941 atctgctcga ttgtccatgg tacacccgtc cgagagagtt tgcgggaatg gcggtgccgg
     3001 acgttctttt gagcggcgac cgcgaggcaa tccgtcggtg gcggcgcgaa caggcgttac
     3061 tgagaacgcg tgaacggagg ccggatctgc tcgcccggcg aaacggcagc gcgcgcgatt
     3121 gatattgcga gttacgaaca cgaatccata tttcggcatt acacaacaca ttcagcgacg
     3181 gcagcgatgt cgtcggctac cgctcagcaa gtgagaaatg cacggtgcgg accgcgaa
//
LOCUS       DSCC01000291            1962 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039520, whole genome shotgun sequence.
ACCESSION   DSCC01000291 DSCC01000000
VERSION     DSCC01000291.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039520"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            23..>1962
                     /locus_tag="ENN56_03590"
     CDS             23..>1962
                     /locus_tag="ENN56_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74603.1"
                     /translation="MQTWPRWLKAALLVSAVLVVIGGIWVVRTTINVVTNLQGIVAEE
                     FEQRVGGTLSASDVSWHWGHIDFRDVLITSPHFPGEVRVTRVRVHISLRAVMRYDLDS
                     AIKRVEVVSPTILLDPSIIDRLKAWRNRPKRDPIESLPEIEFVVGEGKIGFTSSEADS
                     VVPIASSIVGWVHTSDNIAVNLSGSMLSEDVNLQLFGTFPLNGVTPDLQLAVDDVQLG
                     RLQPYLDAHFDGSANLALSIDDSVHAEVGLINAEYRPNDSLHVGPVSALVKLDGNEAR
                     WERVDLLVNGLVLSTDGRVAWGDEPEIDAHAEWSGDVDRLGRFLSERLADLRGNVRGA
                     LELRGSPMNPIVAFDMRSDSLVASGVITKDLVLTGHTEPSSDPDGPVLLAVDRMEVTV
                     SGGRITGGGGGTFRPLDVALHLNPSDIRLNDIPVLYDADIAGRAALSGYARVTSDYRT
                     ASLAFDDIELSVRGRPVPLWKVAAELDTTGHITLYGDAATFTAQAEIVTDSLSETRVA
                     VVAYLDRFIPPVGKTSTYVSGEVSAMIEPTSLVLNGSLVVDTGNRPAFPMDLSLETGL
                     RRSSTPLVINVATPALPVTRLQPDVRATITRVDDGWQFRGDAWRNRIRFTGQAASGDA
                     YTADVRVVNAPAGRAAELAHIE"
BASE COUNT          385 a          516 c          611 g          450 t
ORIGIN      
        1 gtttggcggc atgcctcggt tcatgcagac atggccacgg tggttgaagg cggcactgct
       61 tgtgtccgcc gttttggtcg taatcggcgg aatatgggtt gttcggacca ccatcaacgt
      121 cgtgacgaac ttgcagggta ttgttgctga agagttcgaa caacgcgtcg ggggaacgct
      181 gagtgcgtcc gacgtatcgt ggcactgggg ccacatcgat ttccgcgacg tgttgattac
      241 ctcaccgcac ttccccggtg aagtacgcgt gacccgtgtg cgtgtgcata tctccctgcg
      301 tgcggtcatg cgatatgatc ttgattcggc gatcaaacgt gtggaggtcg tctcgccgac
      361 gatactcctc gatccatcca tcatcgatcg tctcaaagcg tggcgaaatc gcccgaaacg
      421 cgatccgatt gaatctcttc ccgaaatcga attcgtggtt ggagaaggga agatcggatt
      481 tacctcgagt gaagcggaca gcgtcgttcc catcgcttcg agtattgtcg ggtgggtgca
      541 tacgtccgac aacatcgcgg tgaacctcag cggaagtatg ctgagtgagg atgtcaatct
      601 gcagctgttc ggaacgtttc ctctaaacgg tgtgactcca gaccttcaac tcgccgtcga
      661 tgacgtgcaa ctcggtcggt tgcagccgta tctcgacgcg catttcgacg gctcagcgaa
      721 tctcgcgctt tcgatcgacg actcggtgca tgcggaagtc ggtttgatca atgcagaata
      781 ccgcccgaat gacagtcttc acgtgggacc cgtgtcggct ttggtgaagt tggatgggaa
      841 tgaggcgcgg tgggagcgcg tcgatctgtt ggtaaatggg ctggtcttga gtaccgacgg
      901 acgcgttgcg tggggtgatg agccagaaat tgacgcgcac gccgaatggt cgggtgacgt
      961 cgatagactt ggacggtttc tcagtgagcg tctggcggac ttacggggga atgttcgtgg
     1021 agcgctcgaa ctgcgtggtt caccgatgaa tccgatcgtg gcattcgata tgcggtccga
     1081 ttctctggtc gcctcgggcg tcattacgaa agaccttgtg ctcacggggc atacggagcc
     1141 gagtagcgat ccggacggtc cggtactcct cgctgtcgac cgcatggaag tgacggtttc
     1201 cggtggcagg attaccggtg gtggtggcgg tacatttcgt ccgctggatg tcgcgttgca
     1261 tctgaatccg tccgatatcc gtctgaacga cattcccgtt ctgtacgatg ctgatatcgc
     1321 aggtcgagcc gcactttcgg gatacgcgcg ggtaacaagt gactatcgga ctgcgagtct
     1381 cgcatttgac gacatcgagc tcagcgtgcg aggtcgaccg gtgccattgt ggaaagtcgc
     1441 cgcggaactt gatacaactg gacacattac gctttacggg gacgcggcga cgtttaccgc
     1501 tcaggcagag atcgttaccg actctctttc cgaaacacgc gtcgccgtcg tagcgtacct
     1561 cgaccgattc ataccgccag ttggaaaaac atcgacgtac gttagcggcg aagtatcggc
     1621 gatgattgag cctacctcac tcgtgctcaa tggttcgctc gttgtggata ccggtaatcg
     1681 ccccgcgttt ccgatggatc tcagccttga gacgggactc cgacgctcga gcacgccact
     1741 ggtgataaac gttgcaacac cggctttacc ggtaacgcga cttcagccag acgtgcgcgc
     1801 aacgatcacg cgcgtggacg atggttggca gtttcgaggc gacgcgtggc ggaaccgaat
     1861 acgatttacg ggtcaagcgg cgtctggcga tgcgtatacg gctgatgtgc gggtcgtcaa
     1921 tgctcccgct gggcgcgcag cggaactcgc acacatagag ga
//
LOCUS       DSCC01000292            1962 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039523, whole genome shotgun sequence.
ACCESSION   DSCC01000292 DSCC01000000
VERSION     DSCC01000292.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039523"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(414..1553)
                     /locus_tag="ENN56_03595"
     CDS             complement(414..1553)
                     /locus_tag="ENN56_03595"
                     /inference="COORDINATES: protein motif:HMM:PF06977.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74604.1"
                     /translation="MSPIRITAPGKTIVEVFHTNLEDRHARRENNRASERFSIDDAPK
                     IDWCTIILSLCSNGDERASRPVAKACLPKSDRENGKGEWRMNVRPMITRAFAGVVACS
                     VLGLMACSTASDPDPKVVLFPYEYDTVLARGFQEPSGIVWHPLRGTLFVVGDEGDIAE
                     YSVDGTQLAIRRIEREQMGARPDFEGITVIPETGLLYVAVEGAEQILEVSPDDLTILR
                     RFAVAREHEGRTIMAAGGQGFESITFVPRDGDPDGGTFFVANQGFENAPDDDRSVIIE
                     VRLPRRANVSESDDHVVSVPILRVLEPGITDLSGMHYDADRNLLLVLSDSRNALSEIR
                     LDGTIAAVYAIQGRDQEGITIDGDGNVYIAQDSGLILKLRPSVSE"
     gene            1537..>1962
                     /locus_tag="ENN56_03600"
     CDS             1537..>1962
                     /locus_tag="ENN56_03600"
                     /inference="COORDINATES: protein motif:HMM:PF12850.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDS74605.1"
                     /translation="MRIGLISDTHGRLDPAIWEVFDGVDAILHAGDWGEYSLAAVLSS
                     IAPVQSVRGNVDPPTRRFPDRLTTEIGGVSIYLTHMFDTSPASLNAFTNRNPDIGLVL
                     FGHTHSAFVERVAGTVFVNPGSATRPRGGPPSAAIATIED"
BASE COUNT          422 a          590 c          550 g          400 t
ORIGIN      
        1 gagcaacgtg gttgtgctcg tcgtcggcta catcgcgagc tacttcttcc cgaagccggc
       61 tctcgaggaa atccgcggta tgacgtggtg gacccggaag atggaatcac cggtggaagc
      121 cgcagcaagt gaagcgagcg aataagtagg cgcgcgaacg cgagctgacg ctcgagttcg
      181 ttaaggcgaa gacgcttcgc gtctccgagc gtagcgagga gacgcccggc gaatgaattc
      241 gcgggctcgt attgcaaagt ccgcctacgc ggcgaactct cacgtacgtt cgagttcgtt
      301 tcagcgaagg gcgacgccct tcgccctaac gtcttcaagc gtagcgagaa gacgcgcgaa
      361 accaaccgca ccgcgaaacc accttcttgc tacatatccc acgtccccct cgcttactcg
      421 gagacgctcg gccgcagctt caagatcaaa cccgaatcct gcgcgatgta gacattaccg
      481 tcgccgtcga tcgtgatacc ttcctgatcg cggccctgta ttgcgtatac ggctgcgatc
      541 gtgccatcga ggcggatttc actaagagcg ttgcgcgaat cactcagcac gagcaggaga
      601 ttgcggtcgg cgtcatagtg cataccggaa agatcggtga ttcccggttc gagaacgcgg
      661 aggatcggta ccgaaacgac gtggtcgtcc gactccgata cattcgcgcg acgagggagt
      721 cgcacttcaa taatcaccga acgatcatcg tcgggcgcgt tctcgaagcc ctgattcgcc
      781 acgaagaacg taccgccatc gggatctccg tcgcgcggaa cgaacgtgat cgactcgaaa
      841 ccttggccac cggccgccat gattgtgcga ccttcatgtt cccgcgccac ggcgaatcgg
      901 cggagaatcg taaggtcatc cggcgatacc tcgaggattt gctcggcgcc ttcgacagcg
      961 acgtagagca agccggtttc aggaattacc gtaatccctt cgaaatccgg gcgagcaccc
     1021 atctgctcgc gctcgattcg gcgaatcgcc agttgcgtac cgtcgacgga atactccgcg
     1081 atgtcacctt catcaccgac aacgaacaac gtgccgcgca gcggatgcca gacaatcccg
     1141 gacggctcct gaaaacctcg cgcgagtacc gtatcatact catacgggaa caatacaact
     1201 ttcgggtcgg gatcagacgc agtcgaacac gccataaggc cgagcacgct acacgccacg
     1261 actcccgcga aagcgcgggt gatcatcggt cgaacattca ttcgccactc tcctttgcca
     1321 ttctctctat cgctttttgg taaacacgct ttcgcaacag ggcgactcgc tcgttcatca
     1381 ccgttcgaac atagcgacaa gatgatggtg caccaatcga tttttggggc gtcatctatc
     1441 gaaaaacgtt ccgacgcgcg gttgttctct cttcgtgcgt gtctatcttc gagattcgta
     1501 tgaaagacct caacgatcgt tttcccggga gcggtaatgc gaatcgggct catcagcgat
     1561 actcacggac gtctcgatcc tgcgatatgg gaggtgtttg acggcgtcga cgcgattttg
     1621 cacgcgggcg attggggcga gtactcgctt gcggcggtgc tttcatcgat cgcaccggta
     1681 caaagcgtcc gtggcaacgt cgatccgccg acacgtcgtt ttccggaccg acttacgacg
     1741 gaaatcggcg gcgtatcgat atacctcacc cacatgttcg atacgagtcc ggcgtcgttg
     1801 aatgcattca cgaaccgaaa tcccgacatc ggtctcgtac ttttcggcca cacacatagc
     1861 gcgttcgtgg aacgggtggc gggtaccgtg ttcgtgaacc ccggttcggc gacgcgtcca
     1921 cgtggtggtc cgcccagtgc ggctatcgcg acaatcgagg ac
//
LOCUS       DSCC01000293            1629 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1050894, whole genome shotgun sequence.
ACCESSION   DSCC01000293 DSCC01000000
VERSION     DSCC01000293.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1050894"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..685)
                     /locus_tag="ENN56_03605"
     CDS             complement(<1..685)
                     /locus_tag="ENN56_03605"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74606.1"
                     /translation="MKFGICNEMFQNWKLNDVFTYAREIGYDGVEVAPFTIAESVDKI
                     DAAQRKAIRRDADAAGVAIVGTHWLLVSPSGLYITHPDESIRTKTRDYFDELIHFTAD
                     IGGDRMILGSPKQRSLLEGVTYDQAWEWARDLFRDIAKTAEARAVTVCLEPLAPAETD
                     FMNTASDAIRMANAVDSPRVKIILDAKAMSGETKPIPNIIRESVGWVGHFHANDPNLR
                     GPGMGDLDYA"
     gene            complement(682..1221)
                     /locus_tag="ENN56_03610"
     CDS             complement(682..1221)
                     /locus_tag="ENN56_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDS74607.1"
                     /translation="MANESIGILSDTHDNRWALEAILSRFRGEGVTYVIHAGDFVAPF
                     NAKYFSEWSGKFLGVFGNNDGERIGVAKQFADIGPLHVGPHLVRVFGRRFLVMHEPAA
                     LDALGRSGDFDAVIYGHTHVVDIREVPYVAGGGSTLIVNPGEAGGWLHGEATAVILNL
                     DTMHSTLVKVPLYPQEGIV"
BASE COUNT          310 a          449 c          516 g          354 t
ORIGIN      
        1 gtgcatagtc gaggtcgccc atgccgggac cacgcaggtt cggatcgttc gcatggaagt
       61 ggccgaccca accgacactt tcgcgtatga tattcggaat tggtttcgtt tcgccggaca
      121 tggccttcgc atcaaggatg atcttaactc gcggactgtc aacggcgttc gccatgcgga
      181 tcgcatcgga tgcggtattc atgaagtcgg tttccgccgg cgcgagcggc tcgaggcaga
      241 cggtgaccgc gcgcgcttcg gcggtcttcg cgatgtcacg gaacagatcg cgcgcccact
      301 cccacgcctg gtcgtacgtt acgccttcca gtaaactgcg ctgtttgggc gagccgagaa
      361 tcatccgatc gccgccgata tccgccgtaa agtggatcag ctcgtcgaaa tagtcgcgcg
      421 tcttcgtgcg gatgctttcg tcgggatgcg tgatgtagag gccggagggg ctgacgagca
      481 gccagtgtgt tccgacgatg gccacgccgg cggcgtcggc atcgcgccga atggccttgc
      541 gttgcgcggc gtcaattttg tcgacggatt cggcgatggt gaacggggcg acttccacgc
      601 cgtcgtagcc gatctcccgt gcgtaggtga agacgtcgtt gagtttccag ttttgaaaca
      661 tctcattgca aatgccgaat ttcatacgat tccttcctgc ggatagagag gaactttcac
      721 gagtgtggag tgcatggtgt ccagattcag aatgaccgcg gtggcctcgc cgtgcaacca
      781 gcctccggcc tcacccggat tgacgatcag cgtcgagccg ccgccggcga cgtagggcac
      841 ctcgcggata tcgacgacgt gagtgtggcc gtagattacc gcgtcgaaat cgcccgaacg
      901 tccgagagcg tcgagtgctg cgggttcgtg catgacgagg aatcgtcgac cgaaaacgcg
      961 gacaagatgc ggcccgacgt gaagcggtcc gatatcggcg aactgcttcg cgacgccgat
     1021 tcgttcgccg tcgttgtttc cgaacacgcc gaggaatttt ccggaccatt cggagaagta
     1081 tttcgcgttg aatggcgcga cgaaatcgcc ggcgtgaata acgtaggtaa ctccttcgcc
     1141 gcgaaatcgg gagagaattg cttcgagcgc ccatcggtta tcgtgcgtat cggagagtat
     1201 cccgatggat tcgtttgcca tgaatatccc ctcgaaacgt atgccgtaaa tatgtgtaag
     1261 cgtacggtag acccgcgaag ccgtcaaccg ggcgagcgct tcgcttcgca ctcgttaagg
     1321 cgaagcgcga atgcttgcgc attcgttggc ttcgcctgtg tgtcgttgcg cgtaactgaa
     1381 agcagtcgct tcgcgcgtag atgaaaacgg gtgcttcgcc cgtatgtctt cgcgtgctac
     1441 acgagcgagc gtgctacacg acttcgcgtg ctacacgagc gagcgcgact tcgcgacagg
     1501 agttgcgagg tcttctcacg tgcgtaggtg gacaggcgcg tggtcgtgcg gtagatttct
     1561 gtcgcgcagg atataccttg ctgattgttc gtctcgcaac gtggggtgcg gggggaaaca
     1621 accgtgaac
//
LOCUS       DSCC01000294            1534 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055172, whole genome shotgun sequence.
ACCESSION   DSCC01000294 DSCC01000000
VERSION     DSCC01000294.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055172"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
BASE COUNT          343 a          448 c          449 g          294 t
ORIGIN      
        1 cacgaagccc gcgccttgga gcatcgcctc gcgccacgcg cgtccgacaa cgatgtgctc
       61 ctcgccaaac tcgttctcga tggcggggag cgtctccgcc ggccagaagt tccacatccg
      121 atcgcgaatg agcacgccgc tgtacaccgg ctccgacgta tcgagactca cgcgattgac
      181 gcggagaact tccgttacgc tggtgaacga gagaacggta aacgtgagga tgatcaacgt
      241 cgcacaggta agcgccgtgc gaattttccg cttgcgcatg tttgacacac cgagtaaaaa
      301 cgcagtgaac atcgcgctga tacgaccgac gtccgcgctg aggtatccct gctgttcctt
      361 gcgaatctgc tgcatctgcg cggtgaattt gccgatgatc accgatacca caaacaggct
      421 cagcgcgaac acggtgaacg caagcagcac cattggagac gcgacagtga tatcgaatgc
      481 gggatgcact tgctcgatga gcacgaacat ggcgataaag attgcgaaga accagagcgc
      541 ctgattcttt acattcaccg caccgatgag gagccgttcg agaaagaacg cgaacggcaa
      601 caacagcgcc atgtaaaaga gcacaccctt caacgcacca ttcgccgtgc caaggatatc
      661 ggggagcacg cgggattcga gcgcaagcgc ggttcgtgct tcggtgaggt acgtagcgat
      721 atcgcgttcg gcgagtgcac gttctgcgtt ggcgagatgc tccgcggcaa ggtcgtggac
      781 gtcgcgcgtg gcctcgtcga tgatgccgtg ccggacgaga gtcgtaaggc gcgcttcgtc
      841 cagttgccac atgtcccgcg cggcttgata gatcgtattc gtgaccagcg tctcggattc
      901 gaccggaaaa ccgatgccct gtcgatactc cggtcgtgcg atggaatccg gcacgttcgt
      961 gagcatcgcg cgaataccga gaatgccctg tttgaagatg aacttcacgc gcgtctccgg
     1021 ctcaacgaag aacgtgttcg caccgtgtgc aatgtgcgag tagaaatgcc acggtttggc
     1081 gtcggtgcga gcgtcgtaca cctcgccgtt ggagagatac tggaagtaac gctggtcgag
     1141 cacgttcgtc acgctgaccg aaacgcagtc gaaaacgacc atcatccagt cggggaatcg
     1201 gtgcccgacg gtcggttcga atgcgtactg ttcgttaccc agatcaacgg cgtagaagat
     1261 gctcccacta tcggcactca tcttgtatgc gtggagccag tacgcgttgc cccacgcacg
     1321 gaccgatgaa cgcccgtcga taggcaatcc gagtatctcg aagctcccgc gacgatccgt
     1381 gagcgcggtg atgttgtaat gacctcgttc cgcgatgacc accgcgctat ccaccggtac
     1441 atcggccgtc accgagcttg tgcgatcatc gtagagcacg acgccgcgtg cttcggcgaa
     1501 atagtcgcca agtgcgaaat catccggtac atac
//
LOCUS       DSCC01000295            2048 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037300, whole genome shotgun sequence.
ACCESSION   DSCC01000295 DSCC01000000
VERSION     DSCC01000295.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037300"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..498)
                     /locus_tag="ENN56_03615"
     CDS             complement(<1..498)
                     /locus_tag="ENN56_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDS74608.1"
                     /translation="MRILGIESSCDETSAAVFSPEDGLLSNVIASQDEHIRFGGVVPE
                     IASRAHTEWVLPVIRRALVDAEGTLDCIDAIAVTAGPGLVGSLLVGVSAAKGLAFARK
                     LPIVPVHHIEAHLFANRIDHPDLEPPFVALIVSGGHTQLMWVPQWGAYRILGTTRDDA
                     AGEAYD"
     gene            complement(495..1439)
                     /locus_tag="ENN56_03620"
     CDS             complement(495..1439)
                     /locus_tag="ENN56_03620"
                     /inference="COORDINATES: protein motif:HMM:PF07949.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDS74609.1"
                     /translation="MFNLRRLQHNLALKLGALAIALLLWSHVKTERKYETIVEFPLVV
                     SEASGRFVVANELPPTVSVNVVGVGKDLIFRDHHGRVVLRPRISRREAITVDLNMQNI
                     EGVDPSEGIEPISIESPRSLVLDFDYLDTREVAVAPRVDLQLEPGHTLVGQMTVNPAK
                     VRIGGPRQYVRRTDSVATDSISLRGVKQSFTRTVSLRPTSGWNLTVTPEEVTINAEVQ
                     VLLERRFREVPVVVTHIPRGVTARAEPDIVAVDIVGGERLINDLSEDDIKVTIDYRKR
                     FEIGLDDLPLTVTLPEQTRLLRVSPPTVSILIETGSHR"
     gene            complement(1558..>2048)
                     /locus_tag="ENN56_03625"
     CDS             complement(1558..>2048)
                     /locus_tag="ENN56_03625"
                     /inference="COORDINATES: protein motif:HMM:PF03328.12"
                     /note="catalyzes the retro-aldol cleavage of
                     2-keto-3-deoxy-L-rhamnonate to pyruvate and lactaldehyde;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDS74610.1"
                     /translation="VVPFINTAADAKLLTRYAMYPPKGIRGIGPRRAQYLADGPWDYQ
                     RNANEEIVVLIPQIEHIDAVNNLDDICAVEGIGGLFLGPSDLSLSLGHGGEYDHPEVV
                     AAKRQILDASRKHNLPLAVASTGIDDARYWVGEGAKLVMVAGDASLISSGMTRILVQV
                     RT"
BASE COUNT          446 a          565 c          590 g          447 t
ORIGIN      
        1 gtcgtaggcc tcgcctgccg cgtcatctcg cgtcgtaccg agtatgcgat atgctcccca
       61 ttgcggaacc cacatgagct gcgtgtgacc gcctgagacg ataagcgcga caaacggcgg
      121 ttcgaggtcg ggatgatcga ttcggttcgc gaagagatgc gcttcgatat ggtgtaccgg
      181 tacgatcgga agtttgcggg cgaatgcgag tccctttgcc gccgatacgc cgacgagtag
      241 cgaaccgacg agacccggtc cagcagtgac tgcgatggcg tcaatacaat cgagcgtacc
      301 ttctgcgtcg acgagtgcgc gacggataac cggaagcacc cattccgtgt gcgcgcgcga
      361 cgcaatctcc ggaacgactc caccaaagcg aatatgttcg tcctgcgatg cgatcacgtt
      421 ggacagcagg ccgtcttccg gactgaacac ggccgctgaa gtctcatcac acgaactctc
      481 gatgccgagt atcctcatcg atgagaaccc gtttcgatga ggatacttac ggtcggcgga
      541 ctcacgcgta ggagtcgcgt ttgttcaggt agtgttaccg taagggggag atcgtccaat
      601 ccgatttcaa agcgcttacg ataatcgatc gtcactttga tgtcgtcttc ggatagatca
      661 ttgatgagtc gttcaccacc gactatgtcg actgcgacga tgtcgggttc agcgcgagcg
      721 gtaacaccgc gtggtatgtg cgtgactact accggaactt cgcggaaacg gcgctcaaga
      781 agcacctgaa cctcagcgtt gatggttacc tcttccggcg taacggtgag gttccagcct
      841 gatgttggac gaagcgaaac ggtgcgagtg aagctctgct tcactccgcg cagagatata
      901 ctgtcggttg cgacggaatc ggtccgtcga acatactggc gtggtccgcc gattcggact
      961 ttcgcgggat tgacggtcat ttggccgacg agagtgtgtc ccggctcaag ttggaggtct
     1021 actcgcggcg caacggcaac ctcccgcgta tcaaggtagt cgaaatcgag aacaagagaa
     1081 cgtggcgatt caatcgatat tggctcgatc ccttcgctcg gatccacacc ttcgatgttc
     1141 tgcatgttca ggtcgacggt aatcgcttcg cggcggctga ttcgtggtcg aaggacaact
     1201 ctgccatggt ggtcgcgaaa gatcaagtcc tttccgacac cgacgacgtt tactgaaacg
     1261 gtcggtggta actcattcgc gacgacaaac cgtccgcttg cttccgatac gacgagcgga
     1321 aactcgacaa tcgtttcgta tttgcgctcg gtcttcacat gcgaccagag cagcagggcg
     1381 attgccaatg ctccaagctt gagtgcgaga ttgtgctgaa gacgcctcag attgaacatt
     1441 ccgaagaccg tagacggggt tccatataaa taccacagcg ccgagactta ccaacgaact
     1501 tggaagttac ggcgctgctc aaccgacgga aacggtcgat tctatccagg tccgtattca
     1561 ggtgcggacc tgtaccaata tacgcgtcat gccggacgag atgaggctcg cgtcgccggc
     1621 gaccatgaca agcttcgcac cttcgccaac ccaataccgc gcatcgtcaa taccagtcga
     1681 tgcaacggcg agtgggagat tgtgctttcg actcgcatcg aggatttgac gtttcgctgc
     1741 gacaacttcc gggtggtcgt attcaccgcc gtgtccgagc gaaagtgaca gatccgacgg
     1801 cccgaggaag agtccgccga taccttccac ggcgcagatg tcgtcaaggt tatttaccgc
     1861 gtcgatatgc tcgatttgcg ggatgagaac aacgatctct tcgttcgcgt ttcgctgata
     1921 gtcccacgga ccgtccgcga gatactgtgc gcgacgtggt ccgatacctc gaatgccttt
     1981 cggtgggtac atcgcgtatc gcgtgaggag cttcgcgtct gccgcagtat tgatgaacgg
     2041 tacgacaa
//
LOCUS       DSCC01000296            1730 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046876, whole genome shotgun sequence.
ACCESSION   DSCC01000296 DSCC01000000
VERSION     DSCC01000296.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046876"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            24..527
                     /locus_tag="ENN56_03630"
     CDS             24..527
                     /locus_tag="ENN56_03630"
                     /inference="COORDINATES: protein motif:HMM:PF14833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS74611.1"
                     /translation="MVGGTNEHRAACRDILDTFACEIFAMGPNGSGATAKLVVNLVLG
                     LNRLVLAEGLVLGERAGLDTTALLSVLRAGGAYSRVMDTKGEKMLTRDYNAEARLAQH
                     LKDVGLILDLGAQTGTPLVLSAIHEQLLRAAAANGYGDADNAAIIEVLRSMAGGASTN
                     DKSDSQP"
     gene            571..1083
                     /gene="tsaA"
                     /locus_tag="ENN56_03635"
     CDS             571..1083
                     /gene="tsaA"
                     /locus_tag="ENN56_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDS74612.1"
                     /translation="MSSHEMVFRPIGYVRSPYQEQSGTPIQPVFGDDTEATVEIAPEY
                     ADGLCDLVGFERIWLLMWFDRARPAKMIVTPRGDTVERGVFATRAPSRPNSIGLSCVR
                     LLGISGATLRIAGVDALDNTPVLDIKPYIPRIDAYRKSRAGWLDTDEVRARRGMRADE
                     RFDQPRDTET"
     gene            999..>1730
                     /locus_tag="ENN56_03640"
     CDS             999..>1730
                     /locus_tag="ENN56_03640"
                     /inference="COORDINATES: protein motif:HMM:PF01408.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74613.1"
                     /translation="MARYRRGACPSRNACGRTFRSTTRYRNIGLGKRTSFYYTYAKRK
                     RRIMSRLRLGIIGCGGMANAHVSRFEGLGDRMRVTAAVDIDQDKANTVAIKFPGAIAA
                     PDYRMVVDRVDAVLIALPHHLHRDSSVFFLDAGKHVLLEKPMANSEQECLDIMAAAER
                     AKRTLMIAYVMRFDPCVIRLRELLASREVGECFQLSIWTEQHTEREPSNWMCRAETLG
                     GGQLFSHGCHYIDLLLWYLGEPVSGI"
BASE COUNT          369 a          479 c          528 g          354 t
ORIGIN      
        1 tacgccgacg cgagtccgtc gtcatggtgg gtggcacgaa tgaacaccgt gcggcgtgcc
       61 gcgatattct cgacacgttt gcctgcgaaa tattcgcgat gggaccgaac ggatccggtg
      121 ccaccgcgaa gcttgtcgtc aacctcgtgt tggggctaaa tcggcttgtg ctcgccgagg
      181 gacttgtgct tggtgaacga gccggactcg ataccacagc gttgctgagc gtgctccgag
      241 ctggtggcgc gtattcgcgt gtcatggata caaagggcga aaaaatgctc acccgtgact
      301 ataacgcgga agcgcggctc gcgcagcatc tcaaagatgt cggcctgatt ctcgatcttg
      361 gcgcacaaac gggaactccg ctcgtcctgt ccgcgattca cgaacaactc ctccgcgccg
      421 cagcggcaaa tgggtacggc gatgcggata atgccgcgat cattgaggtg ttgcgttcca
      481 tggcgggtgg tgcatcgacg aacgacaaat ccgattcaca gccgtagagt gcggcataca
      541 cgggaaaaac cacacgataa aaggaacgca atgtcttctc acgagatggt gtttcgaccg
      601 atcggttatg tccgatcacc ctatcaggag cagagcggca ctccgattca accggtgttt
      661 ggcgatgaca cggaggcgac agtggaaatt gcgcctgagt atgccgatgg attgtgcgat
      721 cttgtcggtt tcgagcgtat ctggctcctg atgtggttcg atcgcgcacg tccggcgaag
      781 atgatcgtga cgccgcgcgg agacacggtg gaacgtggcg tattcgcgac acgtgcgccg
      841 tcgcggccaa actcgatcgg gctgtcgtgt gtgcgactgc tcggaattag cggcgcgacg
      901 cttcgtatcg ccggagtcga tgcactcgac aacacgccgg tgctcgacat caagccatac
      961 attccgcgaa ttgatgcata tcggaaatcc cgcgccggat ggctcgatac cgacgaggtg
     1021 cgtgcccgtc gaggaatgcg tgcggacgaa cgtttcgatc aaccacgcga taccgaaaca
     1081 tagggctcgg gaagcgcaca tcattctact acacatatgc gaagcgaaag aggcggatta
     1141 tgtcacgctt gaggcttggt attatcgggt gcgggggaat ggcaaacgcc cacgtgagtc
     1201 gctttgaagg actcggcgat cgtatgcggg tcaccgcggc ggtcgacatc gatcaggaca
     1261 aggcaaacac cgtcgcgatc aagtttcccg gtgcgattgc agcaccggat tatcggatgg
     1321 tcgtcgatcg cgtcgacgcg gtactcatcg cgcttccgca tcatcttcac cgcgattcct
     1381 cggtgttctt cctcgacgca ggcaagcacg ttcttctcga aaagcccatg gcgaattccg
     1441 aacaggaatg tctcgatatc atggcggcgg cggaacgtgc gaaacgaaca ctcatgattg
     1501 cgtacgtcat gcggttcgat ccgtgcgtca tccgattgcg cgaattgctc gcatcgcggg
     1561 aggtcggcga gtgcttccaa ctttcgatct ggacggaaca acacaccgaa cgcgagccct
     1621 caaactggat gtgtcgcgcg gagacgctcg gcggcgggca actgttctca cacggatgcc
     1681 actacatcga cctgttactc tggtatctcg gtgaaccggt gagcggcata
//
LOCUS       DSCC01000297            1539 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054949, whole genome shotgun sequence.
ACCESSION   DSCC01000297 DSCC01000000
VERSION     DSCC01000297.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054949"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..815)
                     /gene="carA"
                     /locus_tag="ENN56_03645"
     CDS             complement(<1..815)
                     /gene="carA"
                     /locus_tag="ENN56_03645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:PRK12838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HDS74614.1"
                     /translation="MPEKAYLALEDGTVYEGEGFGATGVRVGEVVFNTSMMGYQEIFT
                     DPSYARQIVTMTYPQIGNYGVNPDDVESPHPWVDGVVVKEYSPYPSNYRMTGNLGDWL
                     HENNVVGISGIDTRMLTKKLRVNGSMKGCIASGEQNPDELVERARGWQGITGLDCVNI
                     VSRKEPQVWTDGVHDLAGSIISKSELKYHVVAYDFGFKESILRLLVHHGAKVTILPAS
                     TPAEEALELKPHGIFLANGPGDPEAVTYAINEIRKLLAESDKTGLPIFGICLGV"
     gene            complement(851..>1539)
                     /locus_tag="ENN56_03650"
     CDS             complement(851..>1539)
                     /locus_tag="ENN56_03650"
                     /inference="COORDINATES: protein motif:HMM:PF01636.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74615.1"
                     /translation="GIPYHPTERDAVEAVRQVALLHRVPVDTIPREWSGWYRAIDTRR
                     LIPETIDRMRSIGTANGEVEAFLDTELAFDPETDAPDPVPVHGDLWRGNWVADRGHIA
                     ALTDFDWLHRGSPMEDLADVILAFASNRDVSPDDAPLVEPPNAVLTDRVVLMLREYEC
                     VRGSLSSESRRLMPDMLRSLWLRHTAWIVRELDSPLHLQIALARAMEFRNWFHEVLPT
                     VLRQWSEKTD"
BASE COUNT          328 a          490 c          396 g          325 t
ORIGIN      
        1 acaccgagac agattccgaa tatcggcaat cccgtcttgt cactttccgc aaggagtttg
       61 cgtatttcgt tgattgcgta cgtcaccgct tcggggtcgc cggggccgtt cgcgaggaag
      121 atcccgtgag gcttcaactc gagtgcctct tccgcaggcg tcgaggccgg caaaatcgtc
      181 accttcgcac cgtgatggac aagcagtcgg aggatgctct ctttgaagcc gaaatcgtac
      241 gccaccacgt ggtatttcaa ttccgatttc gagatgatgc taccggcgag atcgtgcacg
      301 ccgtcagtcc acacctgcgg ctctttccgc gatacgatat tgacgcagtc gagaccggtg
      361 atcccctgcc aacctcgagc acgctccacg agttcatccg gattctgttc gccgctcgcg
      421 atacagccct tcatcgaacc gttcacgcgt agctttttcg tgagcatgcg cgtatcaata
      481 cccgatatac ccacaacgtt gttctcgtgt agccagtcac caaggtttcc cgtcatgcgg
      541 taattgctcg ggtacggact gtattccttc accacgaccc cgtcgaccca gggatgtggc
      601 gattccacgt cgtcgggatt tacaccgtaa ttcccgatct gcggatacgt catcgtcacg
      661 atctgccgcg catacgacgg gtcggtaaat atctcctggt atcccatcat cgacgtgttg
      721 aacaccactt cgccgactcg tacaccggta gcgccaaatc cttcgccctc gtacactgtt
      781 ccgtcttcaa gggcgagata tgctttctca ggcatactac tccgttcctg atactcgatc
      841 cgtatgcgaa tcagtcagtt ttctctgacc actgccgaag aacggtcggc agcacctcat
      901 ggaaccagtt gcggaattcc attgctctcg cgagcgcaat ctgtaaatgt aacggcgaat
      961 cgagctctcg cacaatccac gcggtgtgtc gcaaccacaa tgaacgcaac atatcgggca
     1021 tgagacggcg cgactccgag ctcaaagatc cacgcacgca ctcgtactct cgaagcatca
     1081 aaaccacccg atcggtcaaa accgcattgg gcggttccac gagcggcgcg tcatcgggtg
     1141 aaacatcccg attcgacgcg aacgcaagga tgacgtccgc cagatcctcc atcggtgacc
     1201 cacggtgcaa ccagtcgaaa tccgtgagcg cggcaatgtg cccgcgatcc gcaacccagt
     1261 tccctcgcca cagatcgccg tgcaccggca caggatcggg cgcatccgtt tcagggtcga
     1321 acgcgagttc ggtatcgaga aacgcttcga cctcgccgtt cgccgtgccg atactacgca
     1381 tacggtcgat cgtctcggga atgagccgac gcgtgtcgat agcgcggtac catcccgacc
     1441 actcacgggg aatcgtgtcc acgggcacac ggtgaagcag cgcaacctga cgcaccgcct
     1501 cgactgcgtc gcgctcagtg ggatgatacg gtataccac
//
LOCUS       DSCC01000298            2377 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030453, whole genome shotgun sequence.
ACCESSION   DSCC01000298 DSCC01000000
VERSION     DSCC01000298.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030453"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..959
                     /locus_tag="ENN56_03655"
     CDS             <1..959
                     /locus_tag="ENN56_03655"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDS74616.1"
                     /translation="MVRRPSGIEVGRMRVPIGVVGIIYESRPNVTADAAALCVKAGNA
                     CILRGGREAIHSNVIIARVLREAGTKAGLPDDVIQLIDVTDRETVTLLAQAVDLVDVI
                     IPRGGKGLIRAVVDAAKIPVIKHFDGICHTYVDAEADTNMAVSICYNAKVQRPGVCNA
                     METLLVHRNAAERVLPPLVAKYREAGVELRGDEAVRAIDSKVLPATEEDWKTEYLDLI
                     LSIRIVDNIDDAIRHIRTYGSAHTDAIVTNNVFTARKFVREVDSSSVMVNASTRFSDG
                     GEYGLGAEIGISTDKLHARGPMGANELTTYKWVVYGEGTIRQ"
     gene            974..1294
                     /locus_tag="ENN56_03660"
     CDS             974..1294
                     /locus_tag="ENN56_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDS74617.1"
                     /translation="MVTYIRRNRRRIVVYVALAILVWLFIGGSDGFWAQWRMSSTIET
                     LRRENAELRVENVRLRDQITRLQSDMEYIERIARERYGMAQPGERVYRLVPGASGDEE
                     SIRE"
     gene            1179..2045
                     /locus_tag="ENN56_03665"
     CDS             1179..2045
                     /locus_tag="ENN56_03665"
                     /inference="COORDINATES: protein motif:HMM:PF04367.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HDS74618.1"
                     /translation="MWSISNASPERDTAWRNPENVSTASSRALLETRRVFVNKPDLRN
                     TDSRRLFSKLRLYFFTGLVALAPVVITAYILWYLFFTVDNWLNGFYDSVPFLTIGGQP
                     IPGLGFLTIVLLIILAGVIARNIIGSQILKATESQFTRLPLVRGIYNGAKQLTQAIFG
                     GKRALFQQVVLVPFPTRGVYAIAFYTADAAPEILRKTQDDMVSVFLPTTPNPTSGYML
                     IVRRSDIIPLDMNVEEAVKLVISGGAVIPEDRAMQMIADAASGETANPEPSTQPTPES
                     TTGGTEEPLSTH"
     gene            2068..>2377
                     /locus_tag="ENN56_03670"
     CDS             2068..>2377
                     /locus_tag="ENN56_03670"
                     /inference="COORDINATES: protein motif:HMM:PF11967.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74619.1"
                     /translation="MSNPVTTTALVLSRLPWSETSQIVTVITPDLGRIGAIARGAKRA
                     SGGVGPALEPVTESQIVLTPSPSGTLAHIQSADVQDYFSATKRSLTRIALSLALCELA
                     S"
BASE COUNT          557 a          649 c          656 g          515 t
ORIGIN      
        1 atatggtacg cagaccgagc ggtattgaag tcggacggat gcgtgtgcca atcggcgttg
       61 tcggaatcat ctacgaatcg cggccgaatg taaccgctga tgccgcggcg ctttgcgtga
      121 aggcgggaaa cgcatgcatt ctccgcggag gacgagaagc gattcactca aacgtgatta
      181 tcgcgagagt tctccgagag gcggggacaa aggcaggttt accggacgac gtcatccaac
      241 tcatcgacgt tacggatcgc gaaactgtga cattgctcgc ccaagccgtc gacctcgtcg
      301 atgttatcat tccgcgcgga ggtaaagggc tcatccgtgc agtggtagac gcagcgaaaa
      361 tccccgtcat caagcacttc gacggaatat gtcatacata cgtcgacgct gaggccgata
      421 cgaacatggc ggtatcaatc tgctataacg cgaaggtcca acgtccgggc gtttgtaatg
      481 cgatggagac actcctcgtc caccggaatg cagccgaacg ggtacttccg ccgctcgtcg
      541 cgaaatatcg agaagccggt gttgaacttc gcggcgatga agcggtgcgt gctatcgatt
      601 cgaaggtcct tccggcgacc gaagaagact ggaagaccga gtatcttgat ttgattctgt
      661 cgattcgtat cgtcgacaat atcgacgacg caatacgaca tatccgcacg tatgggtccg
      721 cacacaccga cgcgatcgtc acgaacaacg tctttaccgc gcggaaattc gtacgtgaag
      781 tcgattcgtc aagcgttatg gtgaacgcat cgacacgttt ttcggacggt ggcgaatacg
      841 gtctcggcgc tgagatcggc atttcgacag acaaactcca cgcgcgcgga cctatgggtg
      901 ccaatgagtt gacgacgtac aagtgggtcg tctacggaga gggcacgatt cgtcagtgac
      961 aactgcacag accatcgtaa catacatccg aaggaaccgt aggcgtatcg tcgtgtacgt
     1021 cgctttggcg atactcgtgt ggcttttcat cggtggtagc gacggtttct gggcacaatg
     1081 gcgaatgagt agtacaatag aaacgttgcg acgagaaaac gctgaattac gcgtagaaaa
     1141 cgtgcgttta cgggaccaga taacgagact tcaatcggat atggagtata tcgaacgcat
     1201 cgcccgagag agatacggca tggcgcaacc cggagaacgt gtctaccgcc tcgtcccggg
     1261 cgcttctgga gacgaggaga gtattcgtga ataagccgga tctgcgaaac accgactcac
     1321 gaagactgtt ttcaaagctt cgactctact ttttcaccgg actcgtagcg ctcgcaccgg
     1381 ttgtcatcac ggcgtatatc ctatggtatc tcttttttac cgtcgataac tggctgaacg
     1441 gtttctacga ttcggttccg tttctcacga tcggtggaca gccgattccc ggacttggtt
     1501 ttctcacaat cgttctcttg ataatccttg cgggcgttat tgcacggaat atcatcggaa
     1561 gccagatact caaggccacc gaatcgcagt tcactcgact tccccttgtg cgcggcatct
     1621 ataacggagc gaagcaactc acgcaagcga tatttggtgg aaaacgagcg ttgtttcagc
     1681 aagtggtact cgttccgttt ccgacgcgcg gcgtctatgc aatcgcgttt tacacggcgg
     1741 acgccgctcc cgagatcctt cgaaagacgc aagatgacat ggtgagcgtg ttccttccaa
     1801 cgacgcccaa tccgacatcg ggatatatgc tcatcgttcg tcgttcggat attatccctc
     1861 tggatatgaa cgtcgaagag gccgtcaaac tcgtgatttc cggcggtgcc gtgattcctg
     1921 aagaccgagc gatgcagatg atcgcggacg ccgcaagtgg cgaaacggcg aaccccgaac
     1981 cgtcaaccca gcctactccc gaatcgacga ccgggggtac ggaagaacca ctttcgaccc
     2041 attaacccgc aaacggagcc gtaatccgtg tcgaatcccg tcacaacgac ggcgctcgtt
     2101 ctctctcgac taccgtggtc ggagacgagt caaatcgtca cggtcatcac cccggatctc
     2161 gggcgaatag gcgcgatcgc acgcggggca aagcgtgcgt ccggtggagt aggaccggcg
     2221 ctcgaacctg taacggaatc tcagatcgtt ctcacgccgt ctccttcagg aacccttgcg
     2281 catatccaat cagcagacgt gcaagactac ttctccgcga cgaagcgatc gcttacccga
     2341 atcgccctgt ctttagctct gtgcgagttg gcgagtc
//
LOCUS       DSCC01000299            1848 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042983, whole genome shotgun sequence.
ACCESSION   DSCC01000299 DSCC01000000
VERSION     DSCC01000299.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042983"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
BASE COUNT          363 a          557 c          567 g          361 t
ORIGIN      
        1 acctcaccga cggcaagcgc aagtacatct ggttcccact caccggcgcg gagcagttct
       61 tcgacctcga agcagacccg tgcgaacgga atgacctgtc ggatcatccg gggcgtgaaa
      121 acgagctcgt catgtggcgc ggacgactca tcgagcggct cggtcgccgc gacgacggct
      181 tcagtgacgg gaagcagctc atcaaaaaac agtttgcgct caaccactat atcgaaaacg
      241 gccaatacaa ccacgaatga ccgaacactg cgcggagagc gtccgacgat gaggtcgctc
      301 tccgcgactt attccatgct cccgatcgcg tagtccggga tactcccacc gagcgccgct
      361 gacttcgttt cacccggtgc gagtgtttcc ttacgctccg tattctttct cttcgaggca
      421 tcccgccacg aaaacgacac cgacgtatcg cccgaattgt gccaccgcac gcgtggttcc
      481 ggctcccacg aaacgagcgt ggcgatctgt ttcggcagcc cgtcggggta ttcgacgagt
      541 gggtgttccg cgttgaaaag gcggatggag ccgagcatga gcggtccgaa tacgctgttg
      601 acgtcgccga atcggtctgc gcgaccgtgt cccgacgcga gtgcgccgac gctgtagccg
      661 ccgcagccat gttctcgtcc gtcatcgagt gcatttcgac cgactcgcac gcgctcgccc
      721 gcgatgaaca tcgcgcgggc ggcgtaccgc tcgtcgccgg tgatatgaaa cgcgagcgcc
      781 catgcggcag gtgtcggctc ggtcacttca cgtatgctgt cgccctcggg acgaccccaa
      841 cgcacctgat caaagcggtg accgatgtat ttgttgaata tcagcccgat ctcgcgttgt
      901 tccttttcgt cctgccgttc gaggagagag aaaccggccg cgtcgtatgc cggcaggtcg
      961 ggaagcgcct gtatgatcgc cgcatcgtga ctccggtcgt cggtgattcg gcggtatcgc
     1021 gcgaggtgcg ctgcggaggt tgcattacgc gggtcggcta ccgtctccac aagggtcgga
     1081 acaaggcgtt ccacagcggt gacatatcgg tccgtgcccg tgagaaggta gaggtcgagt
     1141 aggaagtcga tcatgccgga cgcgacgatg agttcgacac gttgctcgag gtcgcgttct
     1201 tcgaaaagtc tggccggata cactgccgga tcatccgtgt ggaagaatcc gacgcgcggc
     1261 aacggctcct cgagtaacgc gtctgcccac gtgtttccat aatcgagaca gaagtcgagg
     1321 tatcgacggc gtccggtcgc aacatacgcg ctgagtgcca tcatggcggg acggatcgag
     1381 tccggctctt cgtagtcgtt cggtggatcg gtcggtacgc ggcaggaacc aatgaaccag
     1441 ctcacaaaac ggtgtcgctc ccaatcatac cagtcgggaa caccatcgac ccagtttccc
     1501 gtatgttccg cggcatcatc gagcagtcga ggcgcgacgg tgccggttgg atcaagcgtg
     1561 tggaatcgcg cgatgaaatg ggtgaacggc tcggtgccat ggtgtacttc gccttcggga
     1621 tagtaaccgt gcacgaaatt cgcgttcgcc cacgcggcga atccgtctcg tagccatgtc
     1681 atgaacccgt gtgcggcgcg gtcgcgtgtg agaaaccagt acgcatccca cgacgtcgta
     1741 aacgagcctt cgtcatgaat gccgtgatag gggattacgc gttgcctgcg cacggaatcc
     1801 gcgatccacg cgccaagttc gtctcggaga tgcgcggcgg cgtgaatc
//
LOCUS       DSCC01000300            1687 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048536, whole genome shotgun sequence.
ACCESSION   DSCC01000300 DSCC01000000
VERSION     DSCC01000300.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048536"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            546..1550
                     /locus_tag="ENN56_03675"
     CDS             546..1550
                     /locus_tag="ENN56_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74620.1"
                     /translation="MTESTTTYHVPARTPPSGWKSFGWNGVTCLIPDAWDVSGIDGTA
                     KQGYFTFDDERSRRFEIKFDRARRWGRPALETTLENYFKAVRKQVGKKGYFEVDHDIE
                     LLDEETIPTEYTYRTYGWTGDVIGRGIIVHCPVCRRVTIAQCLAEKNRVNLRELSTVL
                     TSIRCHSETDTNLWAIFEFAAEVPTNLDLQQNKLQAGLINLDFYGKNRRLVVERVGLV
                     QAILKHTAIDRYVEKIHYKKLRRRRLRFIAEPWHGHDGFRIDGERSRLIYRIPSIGTQ
                     LRNWRGSDHVGGRVWHSVEANRLYIVRAEGRDAQALADATADSIARYGDSHGMSENPS
                     "
BASE COUNT          361 a          496 c          493 g          337 t
ORIGIN      
        1 acgtgattgt gttcgagaaa gagccgggaa aaacggttgt ctcggcgctc cgtcctgatg
       61 tcgccatgca gccggtcggc aacgaacagc tcgcgccgat cgccggtgaa atcagtctac
      121 gcttgaagcg ggcaatcgaa gcggtggagt cgacaaaaat cggttcgtga cgacagccgt
      181 ggtgattgcg catctacact gactacagtc aaaatcggag tcgggcgacg aactcggcgc
      241 cgacttcgca tctccgagaa acgcagaatg atattcggaa taccctatct caagcagata
      301 ctcggtgtcg tcatcggtgc ggcgatcggt tttgcgtatt ggcgctttat cggctgctcg
      361 accggtgcgt gcccaatcac gtcgaatccg tggatcagca cgtcgtatgg cgccgcactg
      421 ggactgctca tcacgtacta agcgcgcgtc gtcgtatcgt gtagccttac gacgccgcga
      481 ccctttgccg tatattccct acagttcatc gatccgcgtt ccgtccacct tttccattga
      541 atcacatgac cgaatcgacc acgacgtatc atgttcccgc gcgtacgccg ccatccggat
      601 ggaaatcatt tggctggaac ggagtaacgt gtctcattcc cgacgcatgg gatgtgagcg
      661 gaatcgatgg cacggcgaaa cagggatatt tcacgttcga cgacgaacgt tcgcggcgct
      721 ttgagatcaa gttcgatcgc gcccgtcgat ggggccggcc ggcgctcgaa acgacactgg
      781 aaaactactt caaagctgtg cggaaacagg tcgggaagaa ggggtatttc gaggtcgatc
      841 acgacattga actgctcgac gaagaaacga ttcccactga gtatacatac cgcacgtacg
      901 gctggaccgg ggatgtcatc gggcgcggta tcattgtcca ctgccccgta tgtcgacgcg
      961 ttaccatcgc gcaatgcctc gccgaaaaga accgcgtgaa tctccgcgaa ctctccacgg
     1021 tgctcacgtc tatccggtgt cattcggaaa ccgacacgaa tctctgggcg atcttcgaat
     1081 tcgctgccga agtgccgaca aacctcgatc ttcagcagaa caaactccaa gcgggcctca
     1141 tcaacctcga tttttacggg aagaatcgac ggctcgtcgt cgagcgagtc ggtcttgtgc
     1201 aggcgattct caagcacacg gccatcgacc gctacgtcga gaagattcac tacaagaagc
     1261 tccgtcgtcg ccgattgcgt ttcattgccg agccgtggca tggtcacgac ggcttccgca
     1321 tcgacggtga gcggtcgcga ctgatctacc gcatcccctc tatcggcacg cagcttcgaa
     1381 actggcgggg tagcgaccac gtcggcggac gcgtgtggca ttccgtcgag gcgaaccggt
     1441 tgtacatcgt gcgcgccgag gggcgtgacg cgcaggcgct tgccgacgca acggcggact
     1501 cgatagcgcg ctacggtgat tcgcacggta tgtcggagaa cccgtcgtaa cacgggtcac
     1561 gcgtcgggga ggcacgatgg ccgtctcgca ctcaccacgt cgcacgccga acattctctg
     1621 gctgatgacg gacgaacagc gcaccgattc gctcggatgc tacgggagcc cgtgggcgag
     1681 cacgagc
//
LOCUS       DSCC01000301            2750 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025103, whole genome shotgun sequence.
ACCESSION   DSCC01000301 DSCC01000000
VERSION     DSCC01000301.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025103"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            57..659
                     /locus_tag="ENN56_03680"
     CDS             57..659
                     /locus_tag="ENN56_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74621.1"
                     /translation="MSRDNGKTWHDAGGTIAGIHAAVAQLSDGRLLAFGRGDEIEGMM
                     PMSISDDMGATWTYSASVFPPVHTGQRPLLIKLKTGELFFAAFSNDPNDESDRSRPKD
                     WMHVTDRTGARRPIRGLYCALSLDDGKTWPFARPVSDDGPEHMIETMDGACRPMSRSQ
                     SEPAGYICGFQGADGMIHLASSRNHYVFNRAWLRQQPPPA"
     gene            complement(663..2282)
                     /locus_tag="ENN56_03685"
     CDS             complement(663..2282)
                     /locus_tag="ENN56_03685"
                     /inference="COORDINATES: protein motif:HMM:PF16347.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HDS74622.1"
                     /translation="MKRFSLSNDNARIPTWTGATSQRCLARTGAREPCVPGSLRLPST
                     RKIPPDWRTAMPTSSNTRTGTHAPHSPNVLFFFTDDQRFDTIRALADEFPHAKQLSTP
                     NLDALVRRGTTFTHAHIPGGTCPAVCMPSRAMLHSGRTLFHLDGCGENIPDEHVTLGE
                     CFRNAGYETFGTGKWHNGPAAFNRSFSHGDEIFFGGMNDHWNVPAYYYDPSGRYESRC
                     AYVENWELSNHVAHRACDHIHAGRHSTDLFTDAAIDFLRNRDVSHPFFCYVSLMAPHD
                     PRTMPERFRRMYNPDEIELPANFMAQHPLDTGALRIRDELLAGFPRTPEEVRQHLAEY
                     YAMISHLDDAFGRLMGALESSGELENTIIVFAGDNGLAVGQHGLMGKQSLYDHSVRVP
                     LVFAGPGIPSGERRDAFAYLSDVFPTLCDLTSCDIPPSVEGLSLAPIITDGCTEGRES
                     LYLAYDRTLRGVRKGRFKLVEYAVDGHRETQLFDLVDDPDELRNLAREDRMEAMLHDL
                     RAELYHLRDVWDDRVHATGRTFWAEYDAANV"
     gene            2300..>2750
                     /locus_tag="ENN56_03690"
     CDS             2300..>2750
                     /locus_tag="ENN56_03690"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS74623.1"
                     /translation="MTRSRYVMIDFVNVHKSFGTLHVLRGIDLTFERGLTTVVLGRSG
                     TGKSVTLKCIVGLLEPDDGEVLVEGKNVVDMDRDELNAWRARCGFLFQGGAMYDSMTI
                     EENLIFPLRNRKDISTSDKTELAKEKLSWVGLSGTLDKFPADLSGGMR"
BASE COUNT          568 a          786 c          843 g          553 t
ORIGIN      
        1 gcatcgttct cccgtgcgat gccgtatcct ctggctcggg tggcacggct ctttggatga
       61 gtcgcgacaa tggtaagacg tggcacgatg ctggtggcac gatcgcgggt attcacgccg
      121 cggtggcaca gctcagtgat ggacgcctgc ttgcgttcgg gcgtggcgat gagatcgaag
      181 ggatgatgcc catgagcatc tccgacgata tgggcgcgac gtggacgtac tcggcaagcg
      241 tcttcccacc ggtgcacacc gggcagcgac cactgctcat caagctgaag accggcgagc
      301 tgttcttcgc ggcgttttcg aacgatccca atgacgaatc cgatcgatca cgcccgaaag
      361 actggatgca tgtaactgat cgtacgggag cacgacggcc cattcgcggc ctctactgcg
      421 cgctttcact tgacgacggg aaaacatggc cgttcgcgcg cccggtctcg gacgacggac
      481 cggagcacat gatcgagacg atggatgggg catgccgccc gatgagtcga tcacagtccg
      541 aaccggccgg ctacatctgc ggattccagg gagctgacgg aatgattcac ctcgcgtcga
      601 gccgcaacca ctacgtattc aaccgcgcgt ggctccgtca acagccgccg cccgcctgat
      661 cattacacgt tcgccgcgtc gtactccgcc cagaatgtgc gaccggtagc atgaacgcga
      721 tcatcccaca catcgcgaag gtgataaagt tccgcgcgta ggtcatggag catggcctcc
      781 atacgatcct ctctcgcgag attccgaagc tcgtccgggt cgtcaacgag atcaaacaac
      841 tgcgtctcac gatgcccatc caccgcgtat tcgacgagct tgaagcggcc ttttcgcaca
      901 ccgcggaggg ttcgatcgta cgcgagatac agactctcgc gaccctcggt acatccgtct
      961 gtaatgattg gcgcgaggct caacccttcg acactcggcg ggatatcgca actcgttaga
     1021 tcgcagagtg tcgggaaaac atccgagagg tacgcgaagg cgtcgcgacg ttccccggag
     1081 ggaatacccg ggccggcgaa gacgagtggc acgcgaacgc tgtgatcgta aagactctgt
     1141 ttccccatca gaccgtgctg tccgacagcg agaccgttat ctcccgcgaa tacgataatc
     1201 gtgttctcca gttcgccgct tgattccagt gcacccatga gccgaccgaa cgcgtcgtcg
     1261 agatgactaa tcatcgcgta gtactcggcg agatgttggc ggacctcctc cggtgtccgc
     1321 ggaaatcccg cgagtagctc gtcacgaatc ctcagtgcgc cggtgtcgag tgggtgctgt
     1381 gccatgaagt tcgccggtaa ctcgatctcg tcggggttat acatccggcg gaaacgctcc
     1441 ggcatcgtgc gcgggtcgtg cggcgccatg agcgacacgt aacagaaaaa cggatgtgat
     1501 acgtcgcgat tgcggagaaa gtcgattgcg gcgtcagtga aaaggtccgt ggaatggcgc
     1561 cccgcgtgga tgtggtcaca cgcgcggtgc gcgacgtgat tcgagagttc ccagttctcg
     1621 acgtatgcgc agcggctctc gtagcgaccg gatgggtcgt agtagtacgc gggcacattc
     1681 cagtgatcat tcattccacc gaagaagatt tcgtcgccgt gggaaaagct ccgattgaac
     1741 gcagccggac cgttgtgcca tttccccgtg ccaaacgtct cgtacccggc gttccggaag
     1801 cactcgccga gggtgacatg ctcatccgga atgttctcgc cgcagccatc gagatgaaac
     1861 agcgtccgac cgctgtgaag catcgcgcga ctgggcatac agactgccgg acacgtacca
     1921 ccggggatgt gtgcatgggt aaacgttgtt ccacgacgta cgagcgcgtc gaggtttggc
     1981 gtcgatagct gtttcgcgtg gggaaactca tcggcgagcg cgcgaattgt atcgaatcgc
     2041 tgatcgtcgg taaaaaagaa cagcacattt ggcgaatgtg gcgcgtgcgt tccggttcgc
     2101 gtattcgaag acgtcggcat ggccgttctc caatctgggg gtatcttccg tgtcgatggt
     2161 agacgcagcg aaccgggtac gcaaggctcc cgcgcgcctg tgcgggcgag gcaccgttgc
     2221 gatgtcgccc ctgtccacgt aggtatgcgt gcgttatcat tagagaggga gaagcgcttc
     2281 atccgaaacc ctgtaacgaa tgacacgttc ccggtacgtt atgatcgatt tcgtcaacgt
     2341 ccacaagtca tttggcacgc tgcacgtgct ccgcggtatc gacttgacgt tcgaacgcgg
     2401 cttgaccacg gtcgtactcg gtcgttcagg aaccggcaag agtgttacgt tgaagtgcat
     2461 cgtgggtctt ctggaaccgg acgacggcga ggtgctcgtc gaggggaaaa acgtcgtcga
     2521 catggatcgt gacgaactca acgcgtggcg agctcgttgt gggttcctgt ttcaaggcgg
     2581 cgcgatgtac gattccatga cgatcgagga aaacctcatc ttcccgcttc ggaatcgcaa
     2641 agacatatcc acatccgaca agacggagct tgcgaaagag aagctgagtt gggtcggact
     2701 gtcggggacg ttggacaaat ttcccgcgga cctctcgggg ggaatgcgca
//
LOCUS       DSCC01000302            2421 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029715, whole genome shotgun sequence.
ACCESSION   DSCC01000302 DSCC01000000
VERSION     DSCC01000302.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029715"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..663
                     /locus_tag="ENN56_03695"
     CDS             <1..663
                     /locus_tag="ENN56_03695"
                     /inference="COORDINATES: protein motif:HMM:PF08450.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74624.1"
                     /translation="LQCDPNDVLWCADMKHGILSITADGVVHEEVTEFEGEPMRGCND
                     CAFDTQGNLYVTAPAGSSAEKPVGEIYCRRANGETVRLDSGFAFCNGIGVRGDDSQLI
                     VAETMTKSLWAYDIVSPGVVKNKRLWAKHPDGGLGPDGMDFDEDGNLLVAHWGGGSID
                     VFDPNGKLIERIKTPFEKLSNVHFGGNDRRSVYITEHDSDGLWLLESWKNPGQLQYCD
                     RR"
     gene            826..>2421
                     /gene="typA"
                     /locus_tag="ENN56_03700"
     CDS             826..>2421
                     /gene="typA"
                     /locus_tag="ENN56_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDS74625.1"
                     /translation="MKRIQQNDRIRNIAIIAHVDHGKTTLVDQLLRQSGTFRPNQEVD
                     DRVLDRMDLERERGITISAKNCAISWHNTKINILDTPGHADFGGEVERALMMVDGAIL
                     LVDASEGPLPQTRFVLQKALGLNLPIIVIINKIDRKDARAEAVLNEVYDLFLDLDANE
                     EQIEFPVLYAVGRDGVAKTTLDGAESDLTTLFEIVLQHVPPPSYDPNEPFQMLVADLD
                     YSDYLGRLAIGKIFHGSAHNQDDLVCVGEDGGIRSLRVTNLQVYDGLALRKVERVEPG
                     DIALLAGIDDVHIGDTITTKETPKRLKRVAVDEPTVSMAFYANTSPLAGRDGHFVQSA
                     KIQERLFKETLRNVSLKLEESPDGDAFVVKGRGEFQMAILIETMRREGYEVAVGRPQV
                     IMREFDGKTLEPIEHLFVDCDEQFIGIVSEKLSRRKGRMINLVNHGTGRARIEFSVPA
                     RALIGYRSEFLTDTKGTGIMNSYLEGYEPHRGEFPSRLSGSLVSANRGDAVAYALYNL
                     EPRGTLFIIPNEPVYEGMIIGEHN"
BASE COUNT          570 a          684 c          679 g          488 t
ORIGIN      
        1 cttcagtgcg atccgaacga cgtgctctgg tgcgcggata tgaagcacgg cattctctcg
       61 atcaccgcgg acggcgtcgt tcatgaggaa gtgacggagt tcgaaggaga accgatgcgc
      121 ggatgcaatg actgcgcatt cgatacacag ggcaatttgt acgtgacagc gccggcgggc
      181 tcaagcgccg agaagccggt tggcgaaatc tattgccgtc gcgcgaatgg cgagacggta
      241 cggctcgatt cgggtttcgc tttctgtaac ggtatcggag ttcgcggcga cgactcgcaa
      301 ttgattgtcg ctgagacgat gacgaagtca ctctgggcgt acgatatcgt gtcgccgggt
      361 gtggtgaaaa acaagcggct ctgggcgaaa catcctgacg gtggtctcgg acccgacgga
      421 atggatttcg acgaggacgg caatctcctc gtcgcgcact ggggcggtgg atcaatcgac
      481 gtgttcgacc cgaacgggaa actcatcgag cgaatcaaga ctccgttcga gaagctctcg
      541 aacgtccact tcggtggtaa tgatcgtcga agcgtctaca taacggagca cgactccgac
      601 ggcctctggc tccttgaatc gtggaaaaac cccgggcaac ttcagtactg cgaccgccgc
      661 taaaacctat gttatgtggc gcgagacgtg ccctaacgtc tcgcgccccc cgttcttcgt
      721 tcactctcct ctcgcgctga acctcgcaaa cacgcggcga attccgtata ttaaatcgtt
      781 ttcccctgcg atgtctctcg aaaacggctt ttcaacgaaa tgacaatgaa gcgtatacaa
      841 cagaatgatc gaatccgcaa tatcgccatt atcgcacacg ttgatcacgg caagacgact
      901 ctcgtcgatc aacttctccg acagagcgga acgttccgac cgaatcagga agtcgacgac
      961 cgcgtgctcg ataggatgga ccttgaacgg gagcgcggaa ttaccatatc cgcgaagaac
     1021 tgcgcgatca gttggcacaa cacgaaaatc aacatcctcg atactccggg tcacgccgat
     1081 ttcggtggtg aagtcgaacg cgcgttgatg atggtggacg gcgcgattct ccttgtcgac
     1141 gcgtcggaag gaccactgcc acagacgcgg ttcgttctcc aaaaggcgct cggactcaac
     1201 ctcccaatca tcgtcatcat taataaaatc gaccgaaaag acgcgcgagc cgaagccgtt
     1261 ctcaacgagg tgtacgacct cttcctcgat ctcgacgcga acgaagaaca gatcgagttc
     1321 cccgtactct acgccgtcgg acgagacggc gttgcgaaga cgacactgga cggagctgaa
     1381 agtgatctga cgacgctctt cgagatcgtt ctccagcatg taccgccgcc gtcgtatgat
     1441 ccgaacgaac cattccagat gctcgttgca gacctcgact attcggacta tctcggccgt
     1501 ctcgcgattg gaaagatttt ccacggttcc gcgcacaatc aggatgacct cgtatgtgtc
     1561 ggcgaagacg gtggaatccg gtctctcagg gtgacaaacc ttcaggtata cgacggactc
     1621 gcattgcgga aagtcgaacg tgtcgagccg ggagacattg cgcttcttgc aggcatcgac
     1681 gacgtgcata tcggcgacac gatcacgacg aaggaaacgc cgaaacgtct taagcgagtc
     1741 gccgtagacg aaccgacggt cagcatggcg ttctacgcga acacatcgcc acttgccggt
     1801 cgcgacgggc acttcgttca atcggcgaag atacaggaac gcctcttcaa agaaacgctg
     1861 cgaaacgttt cgctcaaact cgaggaatca cccgacggtg acgcgttcgt cgtaaaaggg
     1921 cgcggcgagt tccaaatggc gatcctcatc gagacaatgc gtcgtgaggg gtacgaagtc
     1981 gccgttggac ggccgcaggt gatcatgcgc gagttcgacg ggaagacact tgagccgatc
     2041 gaacacctgt tcgtcgactg tgacgagcag ttcatcggta tcgtttcgga gaaactctcc
     2101 cgtcgcaaag gtcgaatgat caatctcgtc aaccacggca ccggccgcgc acgtatcgag
     2161 ttctccgtgc ccgcacgcgc attgatcgga taccggtcgg agttccttac cgatacaaaa
     2221 ggcacgggca tcatgaactc gtacctcgag ggatacgagc cgcatcgcgg cgaatttccg
     2281 tcgaggcttt ccggatcgct cgtttcggca aatcgcgggg atgcggttgc ctacgcgctc
     2341 tacaatctcg aaccacgcgg tacgttgttt atcattccga acgaaccggt atacgaaggc
     2401 atgattatcg gcgagcacaa c
//
LOCUS       DSCC01000303            1943 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040059, whole genome shotgun sequence.
ACCESSION   DSCC01000303 DSCC01000000
VERSION     DSCC01000303.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040059"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(271..1824)
                     /locus_tag="ENN56_03705"
     CDS             complement(271..1824)
                     /locus_tag="ENN56_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74626.1"
                     /translation="MPTPLYWLVFWETALSREHAVSFPRRLVGGTQRSYTDAERRVLD
                     NCVRTALEYAGRVRKEFPDAKLVYGNGYVPFIDALLADGFPRDLIDGLGLDFNMFFGM
                     PERQPGPLHAPFGGLYQLARLQDIHGCAEKPRYLTEAIYCAQEDGWLTEREQADHYVR
                     SHLLGLAADVVNFGMTGELWDPGGWYRYSHYGPVGYLHEPPELNPRESYCAYATMTRV
                     LDRATFDAFVDVGSPSVYVLRFTKRSGEAIYAVWTIRGRRPLAFSGEDTARVTDMFGN
                     VRAEHKTNGRYAVTLTSSPQYIEGVRQLTDVRLGEPEHDAAPASCSEIVRFDRDRWCV
                     IETPDPVFDALVDGPHGVVKAPYAHAALELTIDTSALRISLGTDDTHHPLTVRYGRIA
                     LDAPVELSPDARFLVAEIVGNSSWARLLYTIRDGNGRDWKSILQDEQVDFDGTKYLET
                     PLPDAYRAPAVDPRGYHSWKCDDDGAEPVGPFALTGFTVEARSHVVRGAELYPVVTPE
                     WWVRSICIV"
BASE COUNT          412 a          595 c          563 g          373 t
ORIGIN      
        1 gtacaacgga ctggatttcc ccgatgtcgc gtcgattgcg tgtcccaaac cgatgctgtt
       61 ctacaacggc ctacgcgacg ggttgttcag catcgagagc gtcgaatacg cgtacgaccg
      121 aatgcgtcgc gtgtgggatt cgcagggtgt cggtgaccgg ctcgaaacgc ggctctggga
      181 cgtgcctcac gaattcaacg tcgacatgca ggatgcggcg ttcgcatggt tggatcgatg
      241 gctcccgagt tagccggcga gtcttcacga tcaaacgata cagatactcc gaacccacca
      301 ttcgggcgtc acaaccggat acagctccgc accgcgaacg acgtgcgaac gcgcttccac
      361 ggtaaagccg gtgagtgcga acggtccgac cggttctgcg ccgtcgtcgt cgcatttcca
      421 tgaatgatac ccacgcggat caaccgccgg cgcgcggtat gcatccggca acggcgtctc
      481 aagatatttc gtgccgtcga aatcgacttg ctcatcctga agaatcgatt tccagtcgcg
      541 tccgttaccg tcacgaatcg tatagagtag gcgtgcccaa gagctgttcc cgacgatttc
      601 cgcgacgaga aatcgtgcgt ccggagaaag ctcaaccggt gcatccagag cgatacgccc
      661 atatcgcacc gtgagaggat gatgtgtatc gtcggtacca agcgagatcc gaagcgcgga
      721 cgtgtcaatc gtaagttcga gcgccgcgtg ggcgtatggc gctttcacga cgccgtgcgg
      781 tccatcaacg agcgcatcaa aaaccggatc aggcgtttcg atgacgcacc accggtctcg
      841 atcgaatcgc acaatttcgg aacacgatgc cggagccgca tcgtgttcgg gttcaccaag
      901 tcgcacgtcg gtaagctgac gaacaccctc gatatactga ggcgatgagg tcaacgtaac
      961 tgcgtatcta ccgttcgtct tgtgttccgc tcgaacgttc ccgaacatat cggtcacccg
     1021 tgcagtatct tcgccgctga acgccagcgg acgacgtccc cgaatggtcc acacggcgta
     1081 gatcgcctca cccgatcgtt tcgtgaaccg aagaacatag acgctcggtg agccgacgtc
     1141 gacaaacgcg tcgaaggtcg cacggtcgag aacgcgagtc atcgttgcgt acgcgcagta
     1201 gctttcccga gggttcagtt cgggcggttc gtggagatat cccactgggc cgtagtggct
     1261 ataccggtac caaccacccg ggtcccatag ttcgcccgtc atgccgaagt tgacaacgtc
     1321 tgcggcgaga ccgagcagat gcgaacgaac gtagtgatcg gcctgttcgc gttcggtgag
     1381 ccaaccgtct tcctgcgcgc agtagatcgc ctcggttaaa tagcgcggtt tctccgcaca
     1441 accgtggata tcctgcaacc gcgcaagttg atacaagccg ccgaaaggcg cgtggagcgg
     1501 tcccggctgg cgttccggca tcccgaaaaa catgttgaaa tcgagtccca acccgtcgat
     1561 gaggtcgcgc ggaaagccat cggcgaggag cgcgtcgatg aacgggacgt atccattccc
     1621 gtacacgagt ttcgcgtcgg gaaactcctt ccgcacgcgc ccggcgtatt ccaacgccgt
     1681 ccgcacgcaa ttgtcgagca cgcgccgctc ggcatccgta taggaacgct gcgtgccgcc
     1741 aacaagtcgc ctcgggaagg ataccgcgtg ttcccgactc aacgcggtct cccagaagac
     1801 gagccaatag agcggcgtcg gcatcctccg catccgctcg atcatggcat caatatctgc
     1861 ggtcccgtcg ttgcatgtgt tcgcgatgca cgcaacgttg gcggcggacg ccccaatccc
     1921 gtgctcgcga aggacacgaa tac
//
LOCUS       DSCC01000304            3109 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021272, whole genome shotgun sequence.
ACCESSION   DSCC01000304 DSCC01000000
VERSION     DSCC01000304.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021272"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1419)
                     /gene="bamA"
                     /locus_tag="ENN56_03710"
     CDS             complement(<1..1419)
                     /gene="bamA"
                     /locus_tag="ENN56_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDS74627.1"
                     /translation="MLLLAAILGLLLSNAALAARVENVIVRGNVRSNERLIRATSGIS
                     QGEEIGPALIQQAIRNIYALGVFSEVNLLGETTGVNTLNVIIEVKEYPILNQLTITGE
                     DEIKESDIREKIRILPGQIVSPHAVTRAVEEVLTAYREKGYYLATVDPRQIELEGGRV
                     DLDFVIDERERVQVKNIRFHGNESLPSKKLLDQMETKEDRWWRSADFDDEKYREDKEK
                     ILTFYRTQGFREATIVRDSVSVGDSGKDLYIDIWVEEGDRYRYGSISWNGNELISDQQ
                     IHRLLDLQEGDVYNREAFNEAVFRLASAYQEQGYWAVSIDPIETPTGNSVDVAFSIFE
                     GEPSKVRFIDVVGNDKTKDKVIRREMALIPGDTFRRSDLERSHRNVFYLNFFENVEPD
                     IKPLPSGDVDVIVSVTEKPTGTVNMAVGYGQVDKWVGSIGLSIPNLLGNGQQLDFQWE
                     FGQTRTSFYLSFTEPWVLDTPTS"
     gene            1811..>3109
                     /locus_tag="ENN56_03715"
     CDS             1811..>3109
                     /locus_tag="ENN56_03715"
                     /inference="COORDINATES: protein motif:HMM:PF03065.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HDS74628.1"
                     /translation="MASQNRYFSFVLHSHLPYVVSHGKWPHGVDWLNEATAETYLPLI
                     EAFDRMAKAGISARATLSVTPVLAEQLSDPSFLDEFDAYLEMKVEAAQTDEQYFRKTS
                     DTERERIAQYWQEWYGTLRTTFSDLYNRDVVGQFRRLQDAGHLEIITCAATHGYLALL
                     GRDECVRAQIRTGVEAYEKHFGRPPRGIWLPECAYRPPYHWQPPVESTIKAYDRLGVD
                     AIVSEFGIDYFILDTHLLRGGEAIGQYIDRFEALRTLWAQFEKAYPTADEEREVAPLR
                     PYYTAGVEAGQKPTAFFARHPEASLQVWSGEWGYPGDPRYLDFHKKNFPGGHRYWAVT
                     GRRVDLADKVLYNPDLTDEHLEENADHFADLIGKLFVDVPSDEPAVICAPFDTELFGH
                     WWFEGPRWIEKVLTKMAARTDYQPATGSNALEAVPPREMLE"
BASE COUNT          647 a          874 c          893 g          695 t
ORIGIN      
        1 cgatgtcggc gtgtcgagca cccatggctc ggtaaagctc agatagaagc ttgtgcgggt
       61 ctgtccgaat tcccactgga agtcgagttg ctgaccgttt ccgagcagat tcgggatgct
      121 caatccgatc gaaccaaccc atttgtcgac ctgaccatac cctacggcca tgttgaccgt
      181 gcccgtgggc ttttccgtga cggagacaat cacatcaacg tcgccgcttg gcagtggttt
      241 gatatcgggt tcgacgtttt cgaagaaatt gaggtagaag acgttacgat ggcttcgctc
      301 gagatcggaa cggcggaatg tatcgcccgg aataagggcc atttcgcgcc ggatcacctt
      361 gtccttcgtc ttgtcgttgc ctaccacgtc gataaagcgc accttcgacg gttcaccttc
      421 gaagatcgag aacgcgacat cgacgctgtt gcccgtcggc gtttcgattg gatcgatact
      481 gaccgcccag taaccctgtt cctggtacgc cgatgcaagt ctgaacaccg cctcgttgaa
      541 tgcttcgcgg ttgtagacgt cgccttcctg cagatcgagc aaccgatgta tctgctgatc
      601 gctgatcagc tcgttcccat tccacgaaat cgagccgtac cggtagcggt caccttcttc
      661 gacccatata tcgatgtata agtcttttcc ggaatcgccg accgaaacgg aatcacgcac
      721 gatggtcgcc tcacggaaac cctgcgtgcg gtaaaaggtg agaatctttt ccttatcctc
      781 gcgatatttt tcgtcgtcga aatcggcgga acgccaccag cgatcttcct tggtttccat
      841 ttgatcgagg agcttcttcg acggaagaga ctcgtttccg tggaaacgaa tgttttttac
      901 ctgcacgcgt tcgcgttcat cgatcacgaa atcgaggtca acccgtccgc cttcgagttc
      961 gatctgtcgc ggatcgactg ttgcgaggta gtaacctttc tcccgatacg ccgtaaggac
     1021 ttcttccacg gcgcgagtga cggcatgggg gctcacaatt tgtccgggaa ggatgcggat
     1081 cttttcccgt atatccgatt ctttgatttc atcctcgccg gtgatcgtga gctgattcaa
     1141 gatggggtat tccttcacct cgatgatgac gttcagcgta ttcactcccg tggtttcgcc
     1201 gaggaggttt acctcgctga acacgccgag tgcgtagatg tttcggatcg cctgctgaat
     1261 aagcgcggga ccgatctctt cgccctggct gataccgctc gtcgcgcgaa tgagcctctc
     1321 attggatcgc acgttcccgc gaacgatcac gttttccact cgggcggcaa gtgccgcgtt
     1381 cgaaagaagc agacccagaa tggccgcaag aaggagcatt ttggggtaca accgcacaga
     1441 cgttcctcaa attgccggat gatgccggag tcgaaccgca ccgcgcgcat ggactacgca
     1501 tggatacgga gggcgtatcg cgtacagaat tactaaatgt aggttctcga gcgggatgcc
     1561 gccagtgtcc gtatttggag agctgttata cattcccgtg ccatgtcata cgcatcggat
     1621 aaaatcgtgt tcctgcgtaa cgtgtccgaa cgcagattcg ttgcgcgtcg ctggtcggcg
     1681 aaccgacgcg ttcgaccacg acggatttgc agcggtcgag actttgcaca gtggggtatt
     1741 gcgcgtttcc ttcaaatgac ggaaaactcg ttcggagcac tcgtggtgac ttagacaaag
     1801 gccggaaccg atggcatctc agaaccgcta tttctcgttc gttctccact cgcatctccc
     1861 ctacgtcgtc agtcatggca agtggccgca cggcgtcgac tggttgaatg aagctaccgc
     1921 cgagacgtac ctgccgctca tcgaggcgtt cgaccgaatg gcgaaggcag gtatctccgc
     1981 acgagcgacg ttgagtgtca cgccggtgct cgccgagcag ctatccgatc cgtcgttcct
     2041 cgacgaattc gacgcgtatc tcgagatgaa agtcgaagcg gcacagaccg acgagcaata
     2101 cttccgcaaa acgtccgata ccgaacgcga gcggatcgca cagtattggc aagagtggta
     2161 cggcacgctt cgaacgacct tttcggatct ctacaaccgc gacgtggtcg gtcaattccg
     2221 tcgccttcaa gatgccggac atttggaaat catcacgtgt gcggcgactc acggctacct
     2281 tgcgcttctt gggcgggatg aatgcgttcg cgctcagata cggacgggcg tcgaggcgta
     2341 cgagaaacac ttcggacggc caccgcgcgg tatttggctg ccggagtgcg cgtaccgtcc
     2401 gccgtaccat tggcagccgc cggtggaatc gacgatcaag gcgtacgacc gcctcggcgt
     2461 cgacgcgatc gtaagtgagt tcggaataga ctacttcatc ttggatacgc accttttgcg
     2521 cggtggtgaa gcaatcgggc agtatatcga ccgattcgaa gcgttgcgca cgctctgggc
     2581 gcagttcgaa aaggcatatc cgaccgccga tgaagagcgt gaggttgcgc cactgcggcc
     2641 gtactacacc gcaggcgtcg aagctggtca gaaaccgacg gcgtttttcg cgcggcatcc
     2701 ggaagcgagc ttgcaggttt ggtccggaga gtggggatat ccgggcgatc cgcgatatct
     2761 cgacttccac aagaagaact tcccgggcgg acaccggtac tgggcggtaa ccggtcggcg
     2821 tgtggatctg gccgacaagg tgctctacaa cccggacctc accgacgaac acctcgaaga
     2881 gaacgccgat cacttcgccg acctgatcgg aaaacttttc gtcgatgtgc cgtccgatga
     2941 gccggcggta atctgcgcgc cgtttgatac ggagctcttc gggcattggt ggttcgaagg
     3001 tccgcgctgg atcgaaaagg tgttgacgaa gatggcggcg cgaacagact atcaaccggc
     3061 gacgggctcg aacgcgcttg aagcggttcc gccacgcgaa atgctggag
//
LOCUS       DSCC01000305            2727 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025366, whole genome shotgun sequence.
ACCESSION   DSCC01000305 DSCC01000000
VERSION     DSCC01000305.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025366"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..2722)
                     /locus_tag="ENN56_03720"
     CDS             complement(<1..2722)
                     /locus_tag="ENN56_03720"
                     /inference="COORDINATES: protein motif:HMM:PF05048.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HDS74629.1"
                     /translation="MQVREGVRADDVQLSEYASSEPDTLADEEVLPAYILRRPTGPVT
                     LSNNIIDGASFTGIVLFGVDSSVVVDNNRIENCSNGIAIEKNASPTIRSNVILNNSSA
                     GIVCSNNSNPSIDRTTIIGSATAGIICSNGSSPFITETVIAKNGIGISTTNSSPTIQN
                     STIAMNDFSGIIAYEGARPRVSRSNVRDNGLAGVDNRSSGDITADRVWWGHIVTDQRP
                     APVIDAAVRRARGEEIDAAVSGSVTATNPLDGPSVDAPGTPNQAVSLLLASTANFDDP
                     DPITTITNGDTLWIRMVAVDESPYLEDQGVIIIASTSGDPEGTQRVLNESGPNTGTYE
                     GYVIASLTAGDPNIVVEVQNGDQIRVTTGTVTPLTQSVTFVSKPAIISRLRVNGEVRG
                     ARLTDPTPTFTWQYVDPENDPQAATQLELSTDPVFSTDPVWRHDETGRLLEYTYDGPP
                     LQRGTTYYLRVRANDSYNWGRWRETSFHMNIAPPTPTPAYPEDGAIITSGSGRPEIAV
                     NNVVDADGDEVSYVFQAFYGEDFANARMQARGGDAPVPGDPNRDVTVWKTVPMLWENS
                     TIWWRVKATDGLEDSDWSTPRSFLLNVQNDPPSPFNMTDPRPDSTVFTIHPRFEWEHT
                     YDPDPDETIEFVLTIARDSLFTNMPRSYTVEPRPEPIQYFQIPQQDSLLDNTYYYWKV
                     VAIEDGEVVLVANEEDSELPEVWKFFVDTGNDPPYFQTIPRVTMQEDTPYRVRVGRYI
                     VDPDNTLDQISVTVRGTQNIAAEVVGDKEILLTPKRDWSGGPETVRLEAQDPLHSIGI
                     GEIIVTVVGVNDPPTLQNIPVQTVAEDTDLRLDLNPFADDIDNRVEELTWRAEYDQTK
                     LQITIERGIATIRGTHDFFGDNVPVRFVVTDPGGLSASTSTTVTVTPVNDA"
BASE COUNT          539 a          757 c          787 g          644 t
ORIGIN      
        1 gcgcatcgtt taccggcgtc accgtcaccg tcgtcgatgt cgatgcggag aggccccccg
       61 gatccgttac gacgaagcga acagggacat tatcgccaaa gaaatcatgc gtaccgcgaa
      121 tcgtcgcgat accacgctcg atcgttatct ggagcttcgt ttggtcgtat tccgcacgcc
      181 acgtcaactc ttcgacacga ttgtcgatat cgtcagcaaa aggattcaga tcgaggcgaa
      241 gatcggtatc ttccgcgacc gtctgtacgg ggatgttctg cagcgtcggt gggtcgttta
      301 caccgacaac agtcacgatg atttcgccga taccgatact gtgaagcggg tcttgcgctt
      361 cgagccgaac tgtttccggg ccgcccgacc aatcacgttt cggtgtgagg agaatctcct
      421 tatcgccgac gacctcggcc gcgatgttct gcgtgcctcg gaccgtgacc gatatttggt
      481 caagtgtgtt atccggatcg acgatgtacc gtcccactcg caccctatat ggtgtgtctt
      541 cctgcatggt gacacgtgga atcgtttgga aatacggcgg gtcgttaccg gtatcgacga
      601 agaacttcca tacttccggt agttcactgt cttcttcgtt cgctacgagc acaacttcgc
      661 catcctcaat agcgacgacc ttccagtaat agtacgtgtt gtcgagcaac gaatcctgct
      721 ggggaatctg gaaatactgt atcggttcgg gacgcggttc gactgtgtac gaccgaggca
      781 tgttggtaaa cagcgaatcg cgtgcaattg tgagaacaaa ctcaatcgtt tcgtcggggt
      841 ccggatcgta cgtgtgctcc cattcgaatc gcggatgtat ggtgaatacc gtgctatcgg
      901 gccgggggtc ggtcatgttg aatggactcg gtggatcgtt ctgcacgttg agcaggaatg
      961 atcgtggggt tgaccagtcg ctgtcttcca atccgtccgt tgcctttacc cgccaccaga
     1021 tcgtgctgtt ttcccacagc atgggaaccg tcttccatac ggtcacatca cgattcggat
     1081 ctccgggtac cggtgcatca ccacctcgcg cctgcatccg agcatttgcg aaatcctcac
     1141 cgtaaaacgc ctggaagacg tacgatacct cgtcgccgtc ggcatccaca acgttgttta
     1201 ccgcgatctc cggacgtccg gagccagagg tgattatcgc gccatcttcg ggatatgccg
     1261 gcgtcggtgt cggtggtgca atgttcatgt ggaacgacgt ttcgcgccaa cgtccccagt
     1321 tgtaactgtc gttcgcgcga acgcggagat agtacgtggt gccacgttga agtggcggtc
     1381 cgtcatacgt gtactcgagg agtcgtccgg tctcgtcatg ccgccacacg gggtcggtcg
     1441 agaacacggg gtcagtcgaa agctcgagct gagtagccgc ctgcgggtca ttttccgggt
     1501 cgacgtactg ccacgtgaac gttggtgtcg ggtcggtgag ccgtgcgcca cggacttcac
     1561 cattcactcg gagccgcgaa atgatcgccg gtttgctgac gaacgtaacg gactgagtca
     1621 acggtgttac ggttccggtt gtcacacgaa tctgatcgcc gttctggacc tcaacgacga
     1681 tgttcgggtc gccggcggtc agcgatgcaa tgacgtagcc ctcatacgtt ccggtattcg
     1741 gaccagattc attcagtacg cgctgcgtgc cttccggatc gccgctcgtg cttgcgatga
     1801 taatgacacc ttggtcttcg agatatgggc tctcgtccac ggcgaccata cgaatccaca
     1861 gcgtatcgcc attcgtaatg gttgtaatcg ggtccggatc atcgaaattc gccgtcgatg
     1921 cgaggagcaa cgatacggcc tggttcggag tgcccggcgc gtcaaccgat ggtccgtcca
     1981 gcggatttgt cgcggtaact gaaccgctaa cagccgcatc gatctcttcg ccgcgcgcac
     2041 gtcgaacggc agcgtcgatt actggcgcag gacgctgatc ggtgacgata tgtccccacc
     2101 aaactcggtc ggcggtgata tcacccgacg aacgattgtc gacaccggca agtccgttgt
     2161 cacgcacgtt cgaccgggaa acgcgcggac gcgccccttc atacgcgatg attccgctga
     2221 agtcgttcat cgcgatggta ctgttctgta tcgttgggct ggaattcgtc gtggagatac
     2281 cgatgccgtt tttcgcgatg acggtttccg taatgaatgg agaagacccg ttcgagcaga
     2341 taattcctgc agttgcggac ccgataatcg ttgtgcggtc gatagaggga ttcgagttgt
     2401 tcgaacacac gatgccggcg ctcgagttgt tcaatatcac gttgctgcga atggtcggcg
     2461 acgcgttttt ttcaattgca atgccgttcg agcaattctc gatgcggttg ttgtcaacaa
     2521 cgacggaact atctacgccg aagagcacaa tgccggtgaa gcttgcgccg tcgatgatgt
     2581 tattgctcag ggtgaccgga cccgtcggac gccggaggat gtatgcggga agcacttcct
     2641 catccgcgag cgtatccggc tcagaactcg cgtattcgct gagctgtacg tcatcagcgc
     2701 gtacgccttc gcggacttga atgccga
//
LOCUS       DSCC01000306            1601 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052164, whole genome shotgun sequence.
ACCESSION   DSCC01000306 DSCC01000000
VERSION     DSCC01000306.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052164"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..576)
                     /locus_tag="ENN56_03725"
     CDS             complement(<1..576)
                     /locus_tag="ENN56_03725"
                     /inference="COORDINATES: protein motif:HMM:PF00245.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDS74630.1"
                     /translation="MYNAQTTRARIRRMIIPVVAIAFATFLAGCGTSAPTKSGPKNVI
                     VLIGDGMGFAHVTASSYYETGKPDGLSYQQFPVQYGMSTYSASGIGYDQIQANSDFDY
                     FKKRPTDSGAAATAMGTGKKSYNGAIGVNMDKDPIGNLCETAEKVGKASGVVTSVNFP
                     HATPAGFIAHTERRGNLEDIARQMVFSNAEVI"
     gene            complement(796..>1601)
                     /locus_tag="ENN56_03730"
     CDS             complement(796..>1601)
                     /locus_tag="ENN56_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HDS74631.1"
                     /translation="LYLTWVVNWLIKPFTMFGIAALFFYVIFKSLIPPELAKDYLAGA
                     VLLGAAPCTAMVFVWSHLTKGNPAYTVVQVATNDLIILVAFTPTVAILLGIGGITIPW
                     NTLFISVVLFVVIPLVGGSLTRHWVIERHGADYFNNAFIPKFNNVTIGGLLLTLVLIF
                     SFQGDVILGNPLHIILIAVPLVIQTLLIFSIAYLTSRKLRLSHDIAAPAGMIGASNFF
                     ELSVAVAITLFGAASPAVLATIVGVLVEVPVMLALVRFANKTRDWFPVT"
BASE COUNT          378 a          426 c          467 g          330 t
ORIGIN      
        1 gatcacctca gcgttgctga agaccatctg gcgcgcgatg tcttcaagat tgccacggcg
       61 ttccgtatgc gcgatgaatc cggccggagt cgcgtgcggg aagttcacgc tggtgacgac
      121 gccggacgct ttcccgactt tctcggcagt ttcgcaaagg tttccaatcg gatctttgtc
      181 catgttgacg ccgattgcgc cgttatacga cttcttaccg gttcccatgg ccgtcgctgc
      241 ggcacccgag tcggtcggac gcttcttgaa gtagtcgaag tccgagttgg cttgaatctg
      301 gtcgtacccg atgccggagg ctgagtaggt cgacatgccg tattgtacgg ggaactgctg
      361 atagctcaat ccgtcgggtt tccccgtctc gtagtagctc gacgccgtca cgtgggcgaa
      421 gcccatgccg tcgccaatga gaacgatcac gttcttcggt cccgacttcg tcggtgcgga
      481 agtgccgcat cccgcgagga acgtggcaaa cgcaatagcg acgaccggaa tgatcatccg
      541 gcgaattcgt gctctggtgg tctgcgcgtt atacatttcg accctccgat caatgataaa
      601 ccgtgtagta gagccgtgtc ggtgccgcgt tttttgcggc ttgtccggca cgcaacatta
      661 acacaaatct aacacaatga gcacgtgact caatgcttcg cacgaaactc agtatggatt
      721 atcggttgat tctgcaagga ttgtgtttgg atggatacgg acgcgctgtt ctcggaaaca
      781 ggtggaattg gcacgctacg taacgggaaa ccaatcgcgg gttttattgg cgaaacgaac
      841 gagcgcgagc atcaccggaa cctcaaccaa gacgccaacg atcgtcgcga gaaccgccgg
      901 cgaagcggca ccgaacagcg ttatcgccac ggcaactgag agctcgaaga agttcgacgc
      961 gccgatcata ccggctggtg cggcgatgtc gtgagacaac cttagctttc tgctcgttag
     1021 atacgcgatt gagaaaatca acagcgtctg aataaccaaa ggaaccgcga tcaagatgat
     1081 gtgtaaggga ttcccgagaa tgacatcgcc ttggaatgag aaaatcaaga cgagtgtcag
     1141 cagtagaccg ccgatggtga cattgttgaa tttcgggatg aacgcgttgt tgaagtaatc
     1201 cgcgccgtgt ctctcgatga cccaatgacg ggtgagtgaa ccgccgacga gcggaatgac
     1261 gacgaacagc accaccgaga tgaacagggt attccacggg atggtgatac cgccgatgcc
     1321 gaggagaatg gccaccgtcg gtgtgaacgc aacgaggatg atcaggtcgt tcgtcgcaac
     1381 ttgaacaacc gtgtaggcgg ggttcccctt cgtcagatga ctccatacga acaccatcgc
     1441 cgtgcacggc gccgcgccaa gaagcaccgc accggcgagg tagtccttcg ccaattcagg
     1501 cggtatcagc gacttgaaga tgacgtaaaa gaacagcgcg gcgatgccga acatggtgaa
     1561 gggcttaatc agccagttca cgacccacgt gagatagaga c
//
LOCUS       DSCC01000307            7442 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1006282, whole genome shotgun sequence.
ACCESSION   DSCC01000307 DSCC01000000
VERSION     DSCC01000307.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1006282"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(45..593)
                     /locus_tag="ENN56_03735"
     CDS             complement(45..593)
                     /locus_tag="ENN56_03735"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HDS74632.1"
                     /translation="MKLIRVFLAGLILVIVIAVAFVQLSDTSEKQPDEALSPSDTETV
                     IEQTETEQVVPASPISERVVDLTPPAPVERAEAPPVQSAPKIEAPNRTHADLERMISV
                     NRATIDMCYSLGVMNNPSLSGTIVARIAIEANGRVSDVQWTTNTLRDATFEQCLRDEI
                     NAWRFEPAGDAFVGEYAFTFSR"
     gene            1212..3086
                     /locus_tag="ENN56_03740"
     CDS             1212..3086
                     /locus_tag="ENN56_03740"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00593.22,HMM:PF07715.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS74633.1"
                     /translation="MKHLLVAVVLVLAVTQAVFADMRDTTYVLDPILVTATRTETERR
                     NVPGSVTVLSAERLLATGATDVLSAVAVASPGVFITENGPSGFGLGDGSAGSITIRGV
                     GGGTPNDQVLVMVNGQPDYTGLFGHPLPDAYALNEVERVEIVRGPASALYGSGAMGGV
                     INVITRQATKPGDRITLRTDAGPWNTINGEMSALGRHGAFDYRVSASHTRTDGHRDDA
                     AFERSHVSGDLAWNAGAWTIRTSGATTPFSGSDPGPTYGVAGMDIDVTRNRGSVGVEY
                     DGRWFATEGMFYRSWGEHDFTNGWHSTDYVEGATAQFDYHVIGEGTISTAIDYRRYGG
                     EAEDTSTNPVYDYGSHESSEFSATLSAVLPIGDRLSLVGAARAQNHSEVGWTFSPEAG
                     MSVHITKTLTIRGSASRGFRSPSMRDMALFPASNTDVKPEEVTNIETGAQWKPLDWLA
                     TEVSVFRTTGDNLVVLKTPPPPPPRFVNTGSFTNSGVEAEARWMLSRLHGSLSVSRHE
                     IGKTINTDGGIPVAVIGAPKTTVGFSLGTRVERVRLTVNYRYIAGMYGPDSNDADSLP
                     DRLDDYGVLMLSASRPVFSYLSASVTLRNVLDTKYETMTGYPMPGFHATVGLSATLER
                     "
     gene            3089..3634
                     /locus_tag="ENN56_03745"
     CDS             3089..3634
                     /locus_tag="ENN56_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74634.1"
                     /translation="MSPHVRDTVLAGLVIAMGIGFPIALHLTGGTGIGRMFLPMFLPL
                     AAGAFLLRPVHAMFTGAITPMLSALLTGMPPLAPPVAFLMVGELFVLTGVISILTVRT
                     HLNEWIILTYGVIAERFILAVLAAGLSSAFGLPPQLVTWGSLIVSMPGIVLLFIVVPP
                     VVGRVRKNRRYARGFVGVSAP"
     gene            3600..4241
                     /locus_tag="ENN56_03750"
     CDS             3600..4241
                     /locus_tag="ENN56_03750"
                     /inference="COORDINATES: protein motif:HMM:PF01209.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS74635.1"
                     /translation="MPVGLSACRHHDHARTHYFDTIAEEWDDRYGLEATERIRAWCQS
                     LRMPMNASILDVGCGTGVSSVACAERSDAPRRVVALDLSRGMLREGRKKRGNNRVHWV
                     CATGTRIPIADHSFDVVLALHVWPHIDDGGVALAEWYRVLRPGGMLYLAHLISRDTVN
                     SRHRSANEVREDILPPATDLARFIGGRGFTVVETEDADRYRISAIRNEDGGIG"
     gene            complement(4284..5990)
                     /locus_tag="ENN56_03755"
     CDS             complement(4284..5990)
                     /locus_tag="ENN56_03755"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00581.18,HMM:PF07992.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDS74636.1"
                     /translation="MSRRILIVGGVAAGASAAAKARRMDETAEIIMFERGEYVSFANC
                     GLPYYISRHIVDREQLLVVTPEMFRVRYQIDVRLQHDVVGIDRDKKIVSVHDIAGNKT
                     IEEPYDALILTPGAKPIIPPIPMIDAPNVFPLRTIPDMDRVDAWLKEHNPRRATVIGA
                     GYIGLEMAEALVERGLTVTVVEKVPQVLPPIDPDMAALVEQHMRETGVEVRTGDGVKA
                     FEGDKYVSRVALDSGTVVNTDMVLLSIGVRPDVKLATDAGIALGPTGAIAVSGTMQTS
                     DPHIWAAGDAVESVNGANGKPCWIPLAGPANKQGRIAGANSVGGSLSMGPVFGTSIVR
                     FNKMTAAVTGLNERFCQLQEYDYGVTLIHANQHAGYYPGADLLAVKIIFEKKTGKLLG
                     AQVVGNEGVDKRIDVFATALAANMTAEDLTKLDLAYAPPFGSARDPVIVAGFAAQKQV
                     SGQMKPISSDELHTMIEEGAEVEIIDVRNKDEYDEMHLPNAKLIPLDELRDRLDEIDR
                     SKPVVVYCRIGLRGYLGCRICVQNGIKTRNLAGGIRGWRFELIGRQYAKVVGSTGDVS
                     KA"
     gene            complement(6169..6543)
                     /gene="trxA"
                     /locus_tag="ENN56_03760"
     CDS             complement(6169..6543)
                     /gene="trxA"
                     /locus_tag="ENN56_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015220403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDS74637.1"
                     /translation="MADNVEQKHVGEVTDATWDEVVTNEEEVPVFVDFWAPWCGPCRM
                     VGPVVEQLSGEYEGRMKFVKLNTDENPMVSSQYGIRSIPTLAVFYKGEPIHAMMGAGP
                     LGHMRQFLDASLEKVPGNIIVP"
     gene            6930..>7442
                     /locus_tag="ENN56_03765"
     CDS             6930..>7442
                     /locus_tag="ENN56_03765"
                     /inference="COORDINATES: protein motif:HMM:PF01118.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDS74638.1"
                     /translation="MSRTLRVAVVGATGVAGQQALVSLDGHPWFEVVTLAASPRSAGK
                     KYVDAITDQATGHLRWACDEPLPEWAKDMTVVDAQALNAAEFDLIFTATDSDSAKVLE
                     PKFAQTTPVISTTSAFRYEADVPIFIPGINMDHAAILDSQKKNRGWKGFITPIPNCTT
                     TGLAVSLAPLH"
BASE COUNT         1568 a         2154 c         2054 g         1666 t
ORIGIN      
        1 cgtcctcgcg caccccttgt cgatctggag ccgggttaat ccgattaccg cgagaaggta
       61 aacgcgtact cgcctacgaa cgcatctcct gccggttcga accgccatgc gttgatttcg
      121 tcacgtagac actgctcaaa cgttgcatct cgcagcgtgt tcgtcgtcca ctgaacgtcg
      181 ctgactctcc cattcgcttc gatggcaata cgcgcaacga ttgttccgct tagcgacgga
      241 ttattcatga cgccaagtga gtagcacatg tcaatcgtag cacgattcac cgagatcatc
      301 cgttcgaggt ccgcatgtgt tcggttaggc gcttcgatct tcggtgccga ttgtaccggt
      361 ggcgcttccg ctcgttcgac gggcgcagga ggggtgagat caacgacccg ttcggatata
      421 ggtgaagccg gaacaacctg ctcggtttcc gtctgttcga tgacggtttc ggtgtcggac
      481 ggagagagcg cttcatccgg ctgtttttcc gaggtatcag acaattggac aaacgccacg
      541 gcaatgacga tgacgagaat gagtcccgca aggaagacac gaatcagttt cataggctcc
      601 gcacgatttt cgatttgagg tagaacgaaa cgtgtgatga aaatcgaacc gagtgccgtt
      661 cagtgtcaat gatcacaact gagaacaccg cgaaaacgag agcaatggtc gtcaaccttc
      721 gtgcagagaa atcgggattt cgggggaaag gacatcggac gttcccgcca tcgatgtcaa
      781 actcgagtca cggaacgccg cacaactgtt cgggttcgca caaggccttt tgggggctga
      841 ggggggcggc caaatacagc acccgacgct gtgcggcatt ccatgtccac atcatacata
      901 ccttcgttcg tatccaccgt gattcgcgcc gtgaagaggg gaacccacgg tgcgcgaaga
      961 atacgaacga gatcgataga gcattcttgg aacacacgag tggggaatcc cgcgcgatcc
     1021 atgcaaaagg ggtgcaacct ctgtgccaag tcagattccg ccgtaatcgt ttcggtctcg
     1081 agcgtttttt cttcgttcgt tacccctgag gggttggagt ctcgcgtcga attcgtgtta
     1141 ctattctcgt attcgtgtta ccagctcgat tgtgacgaac gtgccacgtt ttcttccatc
     1201 gggagcaaca aatgaagcac cttcttgtag ctgtcgtact cgtgcttgcc gttacgcagg
     1261 cagtatttgc ggatatgcgt gacaccacgt atgtcctcga cccgatactc gtcactgcca
     1321 cgcgtaccga aaccgagcgc cggaacgttc ccggctcggt cacggtactt tccgctgaac
     1381 gactccttgc caccggagct acggacgttc tctctgccgt cgccgtagct tcgcccggcg
     1441 tgttcatcac ggaaaacggt ccgtccggat tcggactcgg tgatggatcc gctggaagca
     1501 tcacgatacg cggtgtcggt ggtggaacac cgaatgatca ggtactcgta atggtcaacg
     1561 gtcaaccaga ctataccgga ttgttcgggc atccattacc ggacgcgtat gcactgaacg
     1621 aggtcgagcg tgtagaaatt gtgcgcggac cggcatcggc gctatacggt agtggtgcca
     1681 tgggcggtgt catcaacgtc atcacacgcc aagcaacgaa acccggcgac cgtataacac
     1741 tgcgcaccga tgcgggtccg tggaacacga tcaatggcga gatgtcggct ctcggtcgcc
     1801 acggagcgtt cgactatcgc gtctccgcga gccacactcg caccgacggg catcgcgatg
     1861 acgcggcgtt cgagcggtct cacgtttcgg gtgacctcgc gtggaatgct ggcgcgtgga
     1921 ccatacgaac gagcggtgcg acaactccgt ttagcgggag tgacccggga ccgacctacg
     1981 gtgtcgcggg aatggacatc gacgttacgc gaaatcgcgg ctcggtcggt gtcgaatacg
     2041 atggacgctg gttcgcaacg gaaggaatgt tctaccgctc gtggggagaa cacgatttca
     2101 ccaacgggtg gcattcgacc gactacgtcg aaggagcgac cgcgcagttt gattaccacg
     2161 tgatcggaga ggggaccata tccaccgcaa ttgattaccg tcgttatggc ggtgaggcgg
     2221 aggataccag taccaacccg gtatacgatt acggatcaca cgaatcgagc gagttttccg
     2281 cgacgctatc tgcagtacta ccgatcggtg atcgcctgtc cttggtcgga gcggcgcgcg
     2341 cgcagaatca cagcgaagtc ggctggacgt tctccccgga agccggcatg tcggtgcata
     2401 tcacgaaaac actcaccata cggggttcgg caagtcgcgg gttccgtagt ccatcaatgc
     2461 gggatatggc gctttttcca gcgtcgaata ccgacgtaaa gcctgaggaa gtaacgaata
     2521 tcgagactgg tgctcagtgg aagccgctcg actggctcgc tacggaggtg tcggtattcc
     2581 gcacaaccgg tgataacctc gttgtcctaa aaacaccgcc accgccgccg cctcgattcg
     2641 tcaacacagg atcgtttacg aattccggtg tcgaagctga agcgcgctgg atgctcagcc
     2701 gattgcacgg ctcacttagc gtgtcgcggc acgaaatcgg caagactatc aataccgacg
     2761 gcggtattcc cgttgccgtc atcggagcgc cgaagacaac cgtcgggttt tcgctcggta
     2821 cgcgtgtcga acgcgtccgc ctcaccgtaa actaccggta catcgcgggc atgtatggtc
     2881 ccgattcgaa cgatgcggac tcgcttccgg atcgcctcga tgactacggc gtgttgatgc
     2941 tttcagcatc gcgtccggtt ttttcctacc tctccgcgag tgttaccttg cggaacgttc
     3001 tcgacacgaa atatgaaacg atgaccggat acccgatgcc aggtttccat gcgacggtcg
     3061 gactctctgc aactctggag cgctgatcgt gtcacctcac gtgcgagaca cggttctggc
     3121 cggtctcgtg atcgccatgg gtatcggatt tccgattgcg ctccacctga ccggtgggac
     3181 cggtatcggg agaatgtttc tcccgatgtt cctaccactc gccgccggtg catttctcct
     3241 ccgaccggtg catgcgatgt tcacgggagc aataacaccg atgctcagcg cactgcttac
     3301 cggcatgcca ccgcttgcac cgccggtcgc gtttctcatg gtcggcgagc tgttcgtcct
     3361 gaccggcgtg atctcgatac tcaccgtacg gacgcacttg aacgaatgga ttatcctcac
     3421 gtacggagtg atcgcggaac ggttcatact cgcggtgctt gcagcgggtt tgagttcggc
     3481 ctttggatta ccaccgcagt tggttacgtg gggttcgctc atcgtgagta tgcccggaat
     3541 cgtgttgctc ttcatcgttg taccgccggt tgttgggcgg gtacgcaaga atcggagata
     3601 tgcccgtggg tttgtcggcg tgtcggcacc atgatcacgc gcgtacacac tacttcgaca
     3661 cgattgctga ggagtgggac gaccggtacg gactcgaagc gactgagcgc attcgagcgt
     3721 ggtgtcaatc gctccggatg ccgatgaacg catcgatcct cgacgtcgga tgtggcaccg
     3781 gcgtatcctc ggttgcgtgt gccgaacgat cggacgctcc aagacgcgtc gttgctctcg
     3841 atctctctcg cgggatgcta cgcgaaggtc gaaaaaaacg cggtaacaat cgtgtacact
     3901 gggtctgtgc gacggggaca cggattccca tagcggatca ctcgttcgat gtggtactcg
     3961 cactgcacgt ttggccacac attgacgacg gcggcgttgc gctcgcggaa tggtatcgtg
     4021 tccttcgacc gggtggcatg ctgtaccttg cacacctcat atcgcgcgac acagtgaatt
     4081 cgcgccacag atcggcgaat gaagtgcgcg aagacatcct cccgccggca acggatcttg
     4141 ctcgttttat cggtggtcgc ggcttcacgg tagtcgaaac ggaagatgcc gatcgatacc
     4201 gcatcagtgc gatacgaaac gaagacggcg gaatcggata accgatcccg ccgtcttttt
     4261 ctcaacgcgt agccactctt ttcttatgct ttcgacacgt cgccggtgct tccaacaacc
     4321 ttcgcgtact ggcgtccaat aagctcgaac cgccatccac gaatgccacc cgcaagattc
     4381 cgcgtcttga tgccgttttg tacacaaata cggcacccaa ggtatccgcg cagcccaatt
     4441 cggcaataca ccaccaccgg tttgctccga tcgatttcgt cgagtcgatc acggagctcg
     4501 tccaacggaa tgagcttcgc gttcggcagg tgcatttcgt cgtattcgtc tttgttgcgc
     4561 acatcaatga tctcgacttc cgcaccttcc tcgatcattg tatgcaactc atccgacgaa
     4621 atgggcttca tctgaccgct aacctgtttt tgcgccgcga aaccagcaac aatcaccgga
     4681 tcgcgtgccg aaccaaacgg tggcgcgtac gccaaatcga gcttggtaag gtcttccgcg
     4741 gtcatgtttg ccgcaagcgc cgtcgcgaac acgtcgatac gtttgtcaac accttcgtta
     4801 ccgacaactt gcgcaccaag cagtttacca gtcttcttct cgaagatgat tttcactgcg
     4861 aggaggtccg cacccggata gtatccggcg tgttgattcg catgaatgag cgttacgccg
     4921 taatcgtatt cctgcaactg gcagaaacgc tcattcaaac ccgtgaccgc agcagtcatc
     4981 ttattaaacc gcacaatcga cgtgccaaac accggcccca tgctcagcga accaccaact
     5041 gaattcgctc cagcgattcg accttgctta ttcgccggac cagcaagcgg aatccagcac
     5101 ggcttaccgt tcgcaccgtt tacactctct acggcgtcac ccgccgccca aatgtgcgga
     5161 tcgcttgtct gcatcgttcc gctgacggct attgcgcccg tcggtccgag tgcgattccg
     5221 gcatccgtag cgagttttac gtcaggacga actccgatgg ataggagcac catgtccgtg
     5281 tttacgaccg taccgctgtc gagcgcaact cttgacacgt acttgtcacc ttcgaatgcc
     5341 tttacgccgt cgccggtccg tacttcgaca ccggtttccc gcatatgctg ctcaaccagc
     5401 gccgccatat ccggatcaat cggagggagt acctgcggca ccttttcgac aaccgtcacg
     5461 gtcaacccgc gttccacgag cgcctcggcc atctcgagtc cgatatatcc cgcaccgatc
     5521 accgtcgcac gacgtggatt gtgttccttc aaccacgcgt cgacgcggtc catgtcggga
     5581 atcgttcgta atgggaagac attcggtgcg tcgatcatcg gaatgggtgg tatgatcggc
     5641 ttcgcaccgg gagtgagaat cagtgcgtcg tacggctctt cgatggtctt gtttccggcg
     5701 atatcgtgca cggatacaat cttcttatcg cgatcgatcc cgacaacgtc gtgttgcaaa
     5761 cgaacgtcga tctggtaccg aacgcggaac atctccggag ttacaacgag gagctgctcg
     5821 cgatccacga tgtgccgcga aatgtagtac ggaagaccgc agtttgcgaa cgagacgtat
     5881 tcacctcgtt cgaacatgat aatctcagcg gtttcatcca tacgtcgtgc ctttgccgcc
     5941 gcgctcgcac cggccgcgac acctccgacg atcaagatcc gacgactcac ctgtgacctc
     6001 ccatcctttt ggttcttgtc ggacgccgat ttggacgcct gatactatat acgcagaaaa
     6061 aaggccgcga ccaatcgcgg tcgcggcctt gtgtgctgaa tagcgatcaa ttcgcaaaac
     6121 tctaagactc ggcgagtgtc ggttaccatg tctgtaatcg gtatctcgtc aaggaacgat
     6181 aatgttaccc ggcacctttt caagacttgc gtcgaggaac tggcgcatgt gaccgagcgg
     6241 acccgctccc atcatggcgt gaatcggctc gcccttgtag aagaccgcga gcgtcgggat
     6301 actgcgaata ccatactggc tcgataccat cggattctcg tccgtgttca acttgacgaa
     6361 cttcatccga ccctcgtact cgcccgaaag ctgctcaact accgggccaa ccattcgaca
     6421 cggaccgcac cacggcgccc agaagtcgac gaatacagga acttcctcct cgttcgtcac
     6481 gacttcatcc catgttgcat cagttacttc gccaacgtgc ttttgttcaa cgttatcagc
     6541 catcgtatta taccctccac gaatgtcgag attcagtttg ctgctctgtt gttattcgtt
     6601 tgcaacgtat gtgccaatcg gcgtgtggta ttcgataata cgtgtattat caacatcata
     6661 cgccgtattt caggtttccg agagccgcga agtgcctcgt gcggtgtgtt tcatgtaaca
     6721 acgacacgaa acactcgttt cacgtcgata cgctgggaaa tccatttctg tcggtggcgg
     6781 ttgactgagt tttcggtgtg tcgatatact atagagttac cgcgaccgcg cgcggcaaat
     6841 gggaaacgtc cgatcattgg gaaacgaatc gcgccgcgcg aagcgctgat ggagtaatcc
     6901 acgttccctg gaatacgagg agaagcagca tgagtcgcac actgcgcgtt gcagtggtgg
     6961 gtgcgaccgg ggtcgccggc cagcaggcgc tggtatcgct cgacgggcac ccgtggttcg
     7021 aagttgtaac gttggcggca tcaccccgca gcgccgggaa gaaatacgtc gacgcgatta
     7081 ccgatcaggc taccggccac ctccgttggg cgtgcgatga gccgttgccc gaatgggcga
     7141 aagatatgac cgttgtcgac gctcaggcgt tgaatgcggc ggaattcgac ctcatcttca
     7201 cggcaaccga tagcgattcg gcgaaggtcc ttgagccgaa gttcgcgcag acgactcccg
     7261 taatcagtac gacgagtgcg ttccggtacg aagcagatgt gccgattttc atccccggca
     7321 taaatatgga ccacgcggcg attctcgatt cgcagaagaa aaaccgcggt tggaaaggtt
     7381 tcatcacacc aatcccgaac tgtacgacga cggggctggc ggtgtcgctt gctccgttac
     7441 ac
//
LOCUS       DSCC01000308            1543 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054752, whole genome shotgun sequence.
ACCESSION   DSCC01000308 DSCC01000000
VERSION     DSCC01000308.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054752"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            48..>1543
                     /locus_tag="ENN56_03770"
     CDS             48..>1543
                     /locus_tag="ENN56_03770"
                     /inference="COORDINATES: protein motif:HMM:PF17132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74639.1"
                     /translation="MNVAEIRAALKDPGPTYRPMPMWFWNGRIDATEFVNQMDRMWSQ
                     GIRAFFIHPMPQEFRPNDFVSGIEGEYLGSEYMDAITKVVAAAAERDMRVWLYDEGGW
                     PSGPNVGRVVAERPDLKGKVARYNEQGEVEILDRGYPVDLLDREATEIFITQVHEKYA
                     AAVGDHFGSTILGFFTDEPRFAGRVGGAEIPWTSKLPEQFKARKGYDISLGMAILFGL
                     KASRTLDEGKRAQVISDFYDVVTSLWRENWWCVLAEWCHERSLMLTGHLAGEDSLVSH
                     LTNGADFFRAMELVDWPGIDAIWRQIHPDTDRTASNDFPKFASSAAHVTRKKRVISES
                     FAVYGWDLTPDEMRWITNFQFVRGVNALSPMAFYSDTRGARKIGTMSDQFGANPLFGS
                     YKSYADYTGRLAVLASIGSPVIETAIYYPIRSLWFQTDSTITNGLQTISRALLERQID
                     FDYVGDDAFAGATVADGRISVGDCSYSALVFPPTSVVTIEMMRVARDLA"
BASE COUNT          327 a          443 c          443 g          330 t
ORIGIN      
        1 agcgaacgca gccccgttgt tgtagactgg tcgggaaagg aacgctcgtg aacgtcgccg
       61 agatacgcgc cgcgctgaag gacccgggtc cgacctatcg cccgatgccg atgtggtttt
      121 ggaacgggcg tattgacgcg accgagttcg tcaaccagat ggaccgcatg tggtcacaag
      181 gtatccgcgc attcttcatt cacccgatgc cgcaagagtt ccgtccaaac gacttcgttt
      241 ccggtatcga aggtgaatat ctcggttcgg agtacatgga cgccataacg aaggttgtcg
      301 cggcggcggc cgaacgggac atgcgcgttt ggctgtatga cgaaggcggc tggccatccg
      361 gtccgaatgt tggacgcgtc gtggcggaac gtcccgatct caaaggaaag gtcgcgcgat
      421 acaacgaaca gggtgaggtt gaaatcctcg accgtggcta tcccgtcgac ctgctcgacc
      481 gtgaagcaac ggaaatattc atcacgcaag tacacgagaa atacgcggct gcggttggtg
      541 atcatttcgg ttcgacgatt ctcggtttct ttaccgacga accgcgcttt gccggtcgcg
      601 tcggtggtgc ggagattccg tggacatcga agctgccgga gcaattcaaa gcgcggaagg
      661 gatatgatat atccctcgga atggcgattc tcttcggtct caaggcctca cgcacactcg
      721 atgaaggaaa acgcgcacag gtcatcagcg acttctacga cgtcgtcaca tcgctctggc
      781 gcgagaactg gtggtgcgta ctcgccgagt ggtgccacga gcgctcgttg atgctcaccg
      841 gccatcttgc cggcgaggac tcgctcgtaa gccaccttac caacggcgcc gactttttcc
      901 gtgcaatgga actcgtcgac tggcccggaa tcgacgctat atggcgacaa atccatccag
      961 ataccgatcg taccgcgtca aacgactttc ccaagttcgc ttcatctgca gcacacgtta
     1021 cgcggaagaa acgggtcatc tcagagtcgt tcgcagtata cggctgggat cttacgccgg
     1081 acgagatgcg ttggatcacg aatttccaat tcgtgcgcgg cgtaaacgcg ctctcaccca
     1141 tggcgtttta cagcgatacg cgcggcgctc gcaagattgg caccatgtcc gaccaatttg
     1201 gcgcgaatcc gcttttcggt tcgtacaaat cgtatgcgga ctataccggt cgactcgccg
     1261 tgctggcgag catcgggtct cccgtcatcg aaacggcgat ctactacccg atccgatctc
     1321 tctggtttca aactgactca accatcacga acggcctgca aacaatcagc cgcgcccttc
     1381 tcgaacgtca gatcgatttc gactacgtcg gggacgacgc gtttgccggc gcgacggttg
     1441 ccgacggacg gatttcggtc ggtgattgca gctattcggc actcgtattt ccgcctacaa
     1501 gcgtcgttac gattgaaatg atgcgcgtag cgcgcgatct ggc
//
LOCUS       DSCC01000309            4968 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1011277, whole genome shotgun sequence.
ACCESSION   DSCC01000309 DSCC01000000
VERSION     DSCC01000309.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1011277"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..612
                     /locus_tag="ENN56_03775"
     CDS             <1..612
                     /locus_tag="ENN56_03775"
                     /inference="COORDINATES: protein motif:HMM:PF03613.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74640.1"
                     /translation="HLSFFNSNPFVATLGLGALTRIEADARTAGGSPSNEMIERFSVR
                     LSTPLGAVGDELFWAAIRPQMSFLAVLIAIFADVWGAIVFVVGFSIIQYAVRWWLFRR
                     GWELGTRVVEALRDGKTRRLARSSGLIAATCAGAVLVAVVFRMQAFVGDIADAMAIAV
                     FLLATVTGFIIVRVRKSAVLALVIGLAWVGLLSLGAEMFSRFG"
     gene            629..922
                     /locus_tag="ENN56_03780"
     CDS             629..922
                     /locus_tag="ENN56_03780"
                     /inference="COORDINATES: protein motif:HMM:PF00381.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDS74641.1"
                     /translation="MTGKDEIFEADVEVTNPQGIHLRVAAILVRAAAKFPNADFVIKK
                     DDYEVNGKSIMGVLTLVAEQGTQLTLKARGPQGNDLIQTMVELFEAGFDEELA"
     gene            919..2778
                     /gene="ptsP"
                     /locus_tag="ENN56_03785"
     CDS             919..2778
                     /gene="ptsP"
                     /locus_tag="ENN56_03785"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDS74642.1"
                     /translation="MKGPRSSSLPGISDESLLSFSSWLENNKSLGIDPQDHYHSVLHG
                     IAGSPGVAIGRVYLHTNADIWVAARSIDEADIDREIARFRIAVDAVIAEREGLIQRTR
                     ETIGHRQAQIFESHVMLLQDEELIGKTIRRIRNERRNADYCFYIALQEAIDIIDRVAT
                     DPFFKERASDLEDVRASVLHQLAGDSPDDPERDLVSETVVVAHGLTPSDTARMPSDKV
                     IGFITDTGGPTSHASILARSMGIPAIVGCARASSTIAPGQTVIVDGYSGHIYIDPDEV
                     LREQVRRWRERLAERTRILEDLATVPAETVDGHRVSLELNIELPDEITRMSKFPTDGV
                     GLFRTEFLFLARDDWPDEDEQYEVYTRLAKAFVERPVTIRTMDVGGDKLSRRLHVEPE
                     DNPFLGWRAIRISLAQPDVFRTQLRAVLRASAHGNVRLMFPLVTSVDELRQAREHVEQ
                     AKAELDSRAERYDSEMLVGTMIETPASVMIADELAQDSDFFSIGTNDLVQYTLAIDRN
                     NEHVAALFDTSHPAILRLINRTVIAGHRAGIPVYVCGEMAHEPVAAMLLVGLGIDGLS
                     MAPGGIPELKQLIRQFSLADAQQVAREALEQTTGEDVRKLVRHAFSERGILTP"
     gene            2808..3962
                     /locus_tag="ENN56_03790"
     CDS             2808..3962
                     /locus_tag="ENN56_03790"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDS74643.1"
                     /translation="MASSFLFSSESVAEGHPDKVADQISDAVLDAMLKDDPKSRVACE
                     TMVTTGMALVAGEVTTQTYVDIPQLVRNTIRDIGYTHAVMGFDADTCAVITTLDKQSP
                     DIAMGVDEKEGHEQGAGDQGMMFGFATDETPELMPMPIMVAHRLVRRLAEVRKDGTLP
                     YLRPDGKAQVSIRYENDKPVRVETVVVSTQHDEKVTQTQIREDIIAHVVKPSIDDKFL
                     AKETRYHINPTGRFVVGGPQGDCGLTGRKIIVDTYGGFARHGGGAFSGKDPSKVDRSA
                     AYATRWAAKNIVAAGLAKRCEIQVAYAIGVAEPVSIMVDTFGTSQVDEAKIEKAVREV
                     FPLTPKWIVSTLDLLRPIYGVTASGGHFGRSEFPWEQVDRVADLKAAVGW"
     gene            3989..>4968
                     /locus_tag="ENN56_03795"
     CDS             3989..>4968
                     /locus_tag="ENN56_03795"
                     /inference="COORDINATES: protein motif:HMM:PF00370.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDS74644.1"
                     /translation="MTSDVVLGIDLGTSSVRATLIGPDGCPIATATREYPVDSPHPGY
                     SEQHPDTWWQSTCSAVSAALAAADGPEVCAISFSGQMHGTVLCNANDEPIRPAIIWSD
                     QRSSREVTAFRSVLEPGRLAELTANPLAAGFQLATLLWLRGNEPESLDAARHVFLPKD
                     WLRFRMTGVAATDPSDACSTLMFDTANRSWSETVIEASGIDGEKLPRVAESFELSGTL
                     LPTAAHELGLREGVPVFVGAGDTPATAFGNGVVDPGTVQCTIGSGGQLIAPLGTPKYD
                     AELRTHTFCHVPRDRWYAMAAILSAGLSLRWLRGCMFGTDAPSYAELSAMA"
BASE COUNT         1039 a         1360 c         1507 g         1062 t
ORIGIN      
        1 catctgagct ttttcaacag caacccgttc gtcgctacgc tgggactcgg tgcactgacg
       61 cggatcgaag ctgacgcacg cactgcgggc ggttcgccgt cgaacgagat gatcgagcgg
      121 ttctcggttc gcctttcgac accactcggc gcagtcggcg acgagttgtt ctgggcggct
      181 atacgtccgc agatgtcgtt ccttgcggtg ctcatcgcga ttttcgcaga cgtatgggga
      241 gcgatcgtat ttgttgtcgg gttttcgatc attcagtacg cagtacgatg gtggctcttt
      301 cgacgcggtt gggaacttgg aacgcgcgtt gtcgaagcgc tacgtgatgg aaaaaccagg
      361 cgcctcgctc gatcttccgg cttgatagcg gcaacgtgcg ccggagcggt tttggtagcg
      421 gtcgtatttc gaatgcaggc attcgtcgga gacatcgcgg acgcgatggc gattgcggtc
      481 tttcttctgg cgacggttac gggattcatt atcgtacgcg ttcggaagtc ggcggttctt
      541 gcattggtga tcggtctcgc atgggtaggg ttactttcgt taggcgcgga aatgttttcg
      601 cggttcggct gacacatttg gagctgacgt gacggggaaa gacgagattt tcgaagcgga
      661 cgtcgaagtg acgaatccgc agggtattca tcttcgcgtt gcggcgatcc ttgtgcgtgc
      721 ggcggcaaag tttccaaacg ctgatttcgt aatcaagaaa gacgattacg aggtcaatgg
      781 aaagagcatc atgggcgtgc ttacgcttgt agcggagcag ggaacacagc tcacgctaaa
      841 agcgcgcggt ccgcaaggaa atgatttgat acaaacgatg gttgaactct tcgaagcggg
      901 cttcgacgag gaactggcgt gaaaggtccg aggagttcct cgctaccggg catcagcgac
      961 gaaagtctcc tttcgttctc gtcctggctt gagaataaca aatctctcgg tatcgatccg
     1021 caggatcact accacagcgt cctgcacggc atcgccggct cgcccggtgt tgccatcgga
     1081 cgtgtgtacc tccacacgaa cgctgatatc tgggttgccg cgcgctcgat tgacgaagcc
     1141 gatattgatc gtgagatcgc gcgattccgc attgcggtgg acgcggtcat cgcggaacgc
     1201 gaaggtctta ttcagcgaac gcgtgagacg attggccacc gtcaggcgca gatcttcgaa
     1261 tcgcacgtga tgcttctcca agacgaagag ctgatcggta aaacgattcg acgcatcaga
     1321 aatgaacgcc ggaacgcgga ttactgcttc tacatcgcgc ttcaggaagc gatcgacata
     1381 atcgatcgag ttgcaaccga tccgtttttc aaagaacgcg cgtcggatct tgaggacgtg
     1441 cgggcatcgg ttctgcatca gttggccggc gacagtccgg acgatccgga gcgcgatctc
     1501 gtttccgaaa cggttgtcgt tgcacacggc ctgacgcctt cggataccgc acgcatgccc
     1561 tcggataagg tcatcggatt cattacggat accggtggcc cgacgagcca cgcatcgatt
     1621 ctcgctcggt cgatgggaat cccggcgatc gtcgggtgtg cacgtgcttc gtcgacgatc
     1681 gcacccgggc agacggttat cgtcgacggg tattccggcc atatctacat cgatcccgac
     1741 gaggttttac gagaacaggt ccgccggtgg agggagcgtc tcgcggagcg tacccggata
     1801 ctcgaagacc ttgcgacggt gcccgccgaa acggttgacg gtcaccgcgt aagcctcgaa
     1861 ttgaacatcg agctccccga cgaaatcacc agaatgtcga agttccccac cgatggcgtg
     1921 gggttatttc gcaccgagtt tctctttctc gcgcgagatg actggccgga cgaggacgag
     1981 caatacgagg tctacacgcg actcgcgaaa gctttcgtcg agcggcctgt tactatacgg
     2041 acgatggatg tcggcggtga taaactctca cgtcgtcttc acgtggaacc ggaggataat
     2101 ccgtttctcg gatggcgcgc gatcaggata tcacttgcgc aaccggacgt ctttcgtacg
     2161 cagctccgcg cagtccttcg agcgtcggca cacggaaacg ttcgtctgat gtttccgctc
     2221 gtaacgagtg tggatgagtt acgacaagcg cgagagcacg tcgaacaggc gaaggcggaa
     2281 ctcgacagtc gggccgagcg gtacgattcg gagatgctgg tcggaacaat gatcgaaacg
     2341 ccggcgtcgg tgatgatcgc ggatgagctc gcacaggatt cggacttttt cagcatcgga
     2401 acgaacgacc ttgtgcagta cacactcgcc atcgaccgta ataacgagca cgtcgccgcg
     2461 cttttcgaca cgtcacatcc ggcgattctc cggttgatca atcggacggt gatcgcgggc
     2521 catcgtgctg gaatacctgt ctacgtctgt ggcgaaatgg cgcacgaacc ggtagccgca
     2581 atgctgctcg tcgggcttgg catagacggc cttagcatgg cacccggcgg cattccggaa
     2641 ttgaagcaac ttattcggca attctctctc gccgacgctc aacaggtcgc gcgggaggct
     2701 ttggaacaaa caacgggcga ggacgttcgg aagctggtcc gacacgcgtt ctctgagcgg
     2761 ggcattctca cgccctgatc atcggtaatt tacttacgag gagacatatg gcaagctcat
     2821 tcctgttttc gtcagaatcg gtggccgaag gacatcccga caaggtcgcc gatcagatat
     2881 ccgacgccgt actcgatgcg atgctcaagg atgatccgaa gagccgtgtc gcatgtgaaa
     2941 caatggtaac aacgggtatg gcgctcgtcg cgggcgaagt tacgacgcaa acctacgtcg
     3001 atatcccaca actcgttcga aacaccattc gcgatatcgg gtacacccat gctgtaatgg
     3061 gtttcgacgc ggacacgtgc gccgtgatta cgacgctcga taagcaatct cccgatatcg
     3121 cgatgggcgt cgacgaaaag gaaggtcatg aacagggcgc cggtgatcag ggaatgatgt
     3181 tcgggttcgc cactgacgaa actccggaac tcatgccgat gccgatcatg gtcgcacatc
     3241 gtctcgttcg gcgactcgcc gaagtgcgga aagatgggac cttgccgtac cttcgcccgg
     3301 acggtaaggc gcaagtaagt atccggtacg aaaacgacaa gcccgtgcga gtggagaccg
     3361 ttgtcgtttc gacgcagcac gacgaaaagg ttacgcagac acaaattcgc gaggacatta
     3421 tcgctcacgt ggtgaagcca agtatcgacg acaagtttct cgcgaaggaa acgcgctacc
     3481 acatcaatcc gacgggacga tttgtggtag gcggaccaca gggcgactgt ggattgaccg
     3541 gtcgtaagat catcgtcgac acgtatggtg gcttcgctcg acacggtggc ggcgcattca
     3601 gtgggaaaga tcccagcaag gtcgaccgaa gtgccgcgta cgctacgcgc tgggccgcga
     3661 agaacatcgt tgcggcgggg ctcgccaaac gatgcgagat tcaggtcgcc tacgctatcg
     3721 gagtcgcgga gcccgtatcg atcatggtcg atacgttcgg tacgagtcaa gtcgacgaag
     3781 caaagatcga gaaggccgtt cgcgaagtct tcccgctgac cccgaaatgg atcgtcagca
     3841 cgcttgatct tctccgaccg atctacggtg tcacggcgtc aggcggccat ttcggtcgtt
     3901 ccgagttccc gtgggagcag gtagaccgcg tcgcagatct caaagcggcc gtcggctggt
     3961 aagttgttga attcgctgga ggtgccgcat gacatcggat gttgttctcg gcatcgatct
     4021 cggcacctcc agtgtccgcg cgacgctcat cggtcccgac gggtgtccga tcgctaccgc
     4081 cacacgcgaa taccccgttg attctcctca tcccggctat tctgaacaac atccggatac
     4141 gtggtggcag agtacctgta gcgcggtttc cgctgctctc gcggcagctg atggtccgga
     4201 agtatgcgcg atcagctttt ccgggcagat gcacggcaca gtgttgtgca acgcaaacga
     4261 cgagccgatc cggcccgcga ttatctggtc cgaccaacgc tcgtcccgtg aagtaactgc
     4321 ttttcgcagt gttcttgaac ccggtcgact cgctgaactg acggcaaacc cgctcgcggc
     4381 gggctttcag cttgcgacgt tgctgtggct gcgaggaaat gagccggaat ctctcgacgc
     4441 agcccgacac gtatttcttc cgaaagactg gcttcgtttc cgcatgacgg gcgtcgccgc
     4501 aacggatccg tcggatgcat gtagcacgct catgttcgac acggcgaacc gatcgtggtc
     4561 ggaaacggtt atcgaagcgt cgggaatcga tggggagaaa cttcctcggg ttgcggagtc
     4621 gttcgagctg agcggcacgc ttttaccgac cgcagcacat gaactgggac ttcgtgaggg
     4681 tgtaccggta ttcgtcggtg cgggagacac acctgcaacg gcttttggaa acggcgtcgt
     4741 tgatccggga acggttcagt gcacgatcgg atcgggcggg caactcatcg caccgctcgg
     4801 tactccgaaa tacgacgccg agttacgcac ccatacgttt tgtcatgttc cgcgagaccg
     4861 ttggtacgca atggcagcca tactttcggc cggactctct ctgcgatggt tgcgcggttg
     4921 tatgttcggt acagacgcgc catcgtacgc ggagctttct gcaatggc
//
LOCUS       DSCC01000310            4338 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1013608, whole genome shotgun sequence.
ACCESSION   DSCC01000310 DSCC01000000
VERSION     DSCC01000310.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1013608"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            109..369
                     /locus_tag="ENN56_03800"
     CDS             109..369
                     /locus_tag="ENN56_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74645.1"
                     /translation="MGIGGFRIVTMEFDSDPTDTVMEVVFTFEIREGGRVRIEEDQHE
                     MGLFSVDTWVRMMERAGFAVQLRPFPAHADGRDAWLIVGVRR"
     gene            complement(379..1575)
                     /locus_tag="ENN56_03805"
     CDS             complement(379..1575)
                     /locus_tag="ENN56_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDS74646.1"
                     /translation="MITIRSVRAIVTAPGAINLVVVKVETSEPDLYGLGCATFTQRYA
                     AVKAAVEEHLAPLLVGRDVGRIEELQQLMMVHGYWRNGPVLNNAISGVDQALWDIKGK
                     VAAMPLYDLLGGKMREAAAVYRHADGPSLTAVEDRVRAFIDDGVRHVRVQFGGYGGVA
                     CEGVVPVGALPGSYYHPRRYTRDALRALEHIRTTVGDDIELLHDVHERLRPAEAVEFA
                     KEVEPLRLYFLEDLLAPEDIDWFENVRSVCATPLAMGELFCHPREWIPLIANRRIDFI
                     RMHISMIGGLTPARKVAACAEQFGVRTAWHGPGDVSPVGHAINVHLDLVSPNFGIQEW
                     SGFAPEISDVFSGLPEVRRGYVYVSDTPGHGVDIDEAAAASYPPIPRVDTWTQARLPD
                     GSISRP"
     gene            1910..3472
                     /gene="mltF"
                     /locus_tag="ENN56_03810"
     CDS             1910..3472
                     /gene="mltF"
                     /locus_tag="ENN56_03810"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:PRK10859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDS74647.1"
                     /translation="MYTNRRFARMSRRPSELIAGILVVATLVLLGTVLNGCSQNEPVV
                     ESADDSALEMLALRSQKIQTVSFPLDSIVSRDTLRVLTRNSAYTYFLHRGTEMGFEYE
                     LIKDFAEKLGVHVKMIVPPRWDDMIPWLLEGRGDIIAAAMTVTPERAKQVAFSEPYNS
                     VYQVVLTRAGGPRINAPEDLIGKQVHVRAGSSYYRRLQELNKELGGGIKIVAVPEYYD
                     TEYIIELLMDRVIDITVADVNIARLEQSHYDSIRINTRISADENLAWAVRPNADALLD
                     AMNEYIGDLYTSTGRSAFFNILTRRYFLHVQGLDTYKRSLQEFRADGRISRYDHLFEK
                     YATQYGFSFSLITAQAYHESRFNPRASSWVGAKGIMQIMPRTGEALGVKNLSDPEQNI
                     EAGVRYLRELYDRFDSTLHPHDRLAFALASYNVGYGHVRDARLLANEDGLNPNVWQGN
                     VEWMLLNLSRQKYYERTAAGYARGHEAVNYVNDIMNRARTYQALLDGRLEPTLAVDIL
                     AEGFSALAVRPI"
     gene            complement(3617..>4338)
                     /locus_tag="ENN56_03815"
     CDS             complement(3617..>4338)
                     /locus_tag="ENN56_03815"
                     /inference="COORDINATES: protein motif:HMM:PF07944.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74648.1"
                     /translation="TEGLAAIAYAPCTVHTTVNNTDVTLSVATRYPFEDSVIITVHTE
                     TPVRLPLLLRIPTWCESASVIYGTTKETPDAGTFHRIDRMWEDGATISMHLPMTPRVI
                     DRPYGVSVERGPLVYSLFIGEEWRRIHEEATGRELPHGDWEVHPTSAWNFALIIDRKA
                     PEKSFTFEERHVGATPFSPSGTPVICHARGQRVPEWGVARDAADAPPRPPFRVEPEMV
                     ELSLIPYGCTNLRITEFPVAP"
BASE COUNT          943 a         1255 c         1214 g          926 t
ORIGIN      
        1 atcatgatca gcttccttat cgacttcctg ttcttccccg gcgcgggaca cggtatccac
       61 ggctggtgag atcagacgaa cagaacgaac acggaaagcc gtcgatcctt ggggattggc
      121 ggctttcgca ttgtaacgat ggagttcgat tccgacccga cagacacggt catggaagtc
      181 gtgtttacgt tcgagatacg ggaagggggt cgtgtgcgta tcgaggaaga ccaacacgag
      241 atggggttgt tttccgtcga tacgtgggta cgcatgatgg agcgcgccgg gtttgccgtg
      301 cagttacgtc cgtttcccgc acacgccgat ggccgagacg cgtggctgat tgtcggcgtg
      361 cgaaggtaat ctccagcctt acggtcgcga tatgctccca tcgggcagcc gcgcctgtgt
      421 ccacgtgtcc acgcgcggga ttggtggata cgatgcggca gccgcttcgt cgatatccac
      481 gccgtgcccc ggggtatccg acacgtacac gtacccgcgt cgcacttccg gaaggccgct
      541 gaacacgtcg ctgatctccg gtgcgaatcc ggaccactcc tgaattccga aattcggact
      601 gacgagatcg aggtgcacgt tgatcgcgtg tcctaccggc gagacgtcgc ccgggccatg
      661 ccacgcggtt cgcacaccaa actgttcggc acacgcggca actttccgcg ccggtgtcaa
      721 tccgccaatc atgctgatgt gcattcggat aaaatcgatg cgacgattgg cgataagcgg
      781 gatccactcg cgcggatggc agaacaactc acccatcgcg agcggcgtcg cacataccga
      841 gcgaacattt tcgaaccagt cgatatcttc aggtgcaagc aggtcctcaa ggaagtaaag
      901 tcgcaacggt tcgacttcct ttgcgaactc gaccgcttcg gcaggtctga gtcgctcgtg
      961 cacgtcgtga agaagctcga tatcgtcgcc gacggtggtg cggatgtgct cgagtgcgcg
     1021 gagtgcatcg cgcgtgtagc ggcgaggatg gtaatatgaa ccgggtagcg caccgacggg
     1081 tacgactcct tcgcatgcga cgccgccgta gccaccgaat tggacgcgga catgccgcac
     1141 gccgtcgtca atgaacgcac gcacccgatc ttcgacagca gtgaggctcg gtccgtcggc
     1201 atgtcgatac accgccgcgg cttcacgcat ttttccgccg agtaggtcgt acaacggcat
     1261 ggctgcgact ttgcccttga tatcccacag tgcctgatca acgccggaaa tcgcgttgtt
     1321 aagcaccggt ccgtttcgcc agtatccatg caccatcatc aactgctgaa gctcttcgat
     1381 acggccgacg tcgcgtccga cgagcagtgg cgcgagatgc tcctcaacag cggctttcac
     1441 tgctgcgtac cgctgggtga acgttgcaca accaagacca tacaaatccg gctcggatgt
     1501 ctcaactttg accaccacca gattgatagc acccggtgca gtaacaatgg ctcgcacact
     1561 gcgaatcgtg atcatacggc ctccataaag tgatcgatgt attgcactgt aaatatctat
     1621 acataaagcg tttatacata aataccgatg acgcgttgca cctgttccga tatgagacac
     1681 gcacccctca ttccggcctc tttgctgtgc tattccaccc ggtaagtctc gcgctatccg
     1741 attgtaacga ggcacgttac ggcgtagaaa aacatttatg gcacgatagt tgcgttcgtt
     1801 agaaacggtt taagcgttac ccgatcgaaa ttccgtcggt gacctaccgg aacgctggag
     1861 tagggctcac cgactgtaac gcgaagttta tcataacaaa agaggtttta tgtataccaa
     1921 ccgtcgattc gccagaatgt cgcggagacc gagcgagctg attgccggta tcctcgttgt
     1981 cgcgaccctg gtactcctcg gtacggtcct gaacggctgc tctcagaacg aacccgtagt
     2041 ggaatcggct gacgacagcg cgttggaaat gcttgcgctg cgctcacaaa aaatacagac
     2101 agtatcgttt ccgctcgact ccatcgtttc acgcgacaca cttcgcgtac ttacacggaa
     2161 cagcgcctac acgtatttcc ttcaccgcgg taccgagatg ggcttcgagt acgagctgat
     2221 caaagatttc gcggaaaaac tcggcgttca cgtgaaaatg atcgttccgc cacgatggga
     2281 cgacatgatt ccgtggctgc ttgaaggtcg tggtgatatc atcgccgccg caatgaccgt
     2341 gactccggaa cgagcgaaac aggtagcgtt ttcggaaccg tacaatagcg tttaccaggt
     2401 tgtgttgaca cgcgccggcg gcccacgaat caacgccccc gaggatctta tcgggaagca
     2461 ggttcacgtc agggccggaa gttcttatta ccgccgactt caggagctta acaaggagct
     2521 tggcggcgga atcaagatcg tcgcggtgcc ggaatactac gacacggaat acatcatcga
     2581 attgctcatg gaccgtgtca tcgatatcac cgtcgcagac gtcaacatcg cgcgcctcga
     2641 gcaaagtcat tacgattcga ttcgcatcaa tactcgtatc agcgccgatg agaacctggc
     2701 atgggccgta cgcccgaatg ccgacgcatt gctcgacgcg atgaacgaat acatcggcga
     2761 tctgtataca tcgacggggc gctcggcgtt ctttaatatt ctcacccgac gctacttcct
     2821 gcacgtacag ggattggaca cgtacaaacg ttcgctccag gagttccggg ccgacggtcg
     2881 tatttctcgc tacgaccacc tattcgagaa gtacgcgaca caatacggtt tctcgttttc
     2941 actgattacg gctcaagcgt atcacgagtc gcgattcaat ccacgtgcgt cgagctgggt
     3001 cggtgccaag ggaatcatgc agattatgcc aagaaccggc gaagcgctgg gtgtaaaaaa
     3061 cctcagtgac ccggaacaga acatcgaggc cggtgtgcga tatctgcgcg agctgtacga
     3121 ccggtttgac tcgacacttc atccgcacga ccgactcgcg ttcgcgctcg cgtcgtacaa
     3181 cgtggggtac gggcacgtac gcgatgcacg tctgcttgcg aatgaggacg gattgaatcc
     3241 gaacgtgtgg cagggcaacg tcgagtggat gcttctgaat ctttcgcgtc agaaatacta
     3301 tgagcgcacc gccgccggat atgcacgtgg tcatgaggcc gtgaattacg taaacgatat
     3361 catgaaccgt gcgcgcacat atcaagcgct tctcgacggg cggcttgaac cgactctcgc
     3421 tgtagatatc ctcgcagaag gcttctccgc gctcgcagta cgaccaattt aggaacaccc
     3481 gtcatctgga acgacaaccg gatcgtaatc gcccataggc aataacgatc cggttccggt
     3541 tttcctgcca gattgctgct cattcggcag gctttatctc tacctcgtgc aatatccgca
     3601 tgttatacac gctgcgctac ggtgccacgg ggaattccgt gatccgaagg ttcgtgcagc
     3661 cgtaaggaat cagcgacaac tcgaccatct ccggctcaac gcgaaacggc ggacgtggcg
     3721 gcgcgtccgc agcatcgcgc gcgacgcccc attcgggtac gcgttgccct ctcgcatggc
     3781 agatcaccgg tgttcctgac ggagagaacg gcgttgcgcc gacgtgtcgc tcctcgaagg
     3841 taaacgactt ctccggcgct tttcggtcga tgatcagcgc gaagttccac gcgctcgtcg
     3901 gatgtacttc ccaatcgccg tgcggaagct cccgtccggt cgcctcctcg tgaatgcgac
     3961 gccattcctc gccaatgaag agtgagtaga caagcggtcc acgttcgacc gacacgccgt
     4021 acggacggtc gatcacgcgc ggtgtcatcg gcaggtgcat cgatatcgtc gcaccgtctt
     4081 cccacatccg atcgattcga tggaatgtgc ccgcgtcggg cgtttccttg gtcgtcccgt
     4141 agatcacgct cgcagactcg caccatgtcg gtatgcgcaa gagcaggggc agacgaaccg
     4201 gtgtctccgt atgaacggta atgataacgg agtcctcgaa ggggtaccgg gttgcgacgc
     4261 tcagagtcac gtcggtgtta tttaccgttg tatgcacggt acagggcgcg tatgcgattg
     4321 cggcgagtcc ttccgtgc
//
LOCUS       DSCC01000311            4937 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1011377, whole genome shotgun sequence.
ACCESSION   DSCC01000311 DSCC01000000
VERSION     DSCC01000311.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1011377"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..103)
                     /locus_tag="ENN56_03820"
     CDS             complement(<1..103)
                     /locus_tag="ENN56_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDS74649.1"
                     /translation="MRAVVQRVSEASVTVDGERIGEIGAGLLVLVGVA"
     gene            complement(183..1208)
                     /locus_tag="ENN56_03825"
     CDS             complement(183..1208)
                     /locus_tag="ENN56_03825"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74650.1"
                     /translation="MAITSREIVQQSLAFASPPRIPYAMTNGFPSDFRRVSRKPAPNR
                     KQQPWTQRDGYWNMIDEWGNEWRRLENITKGEVYRGVIEQGWELLETYEWPDIDRAEL
                     YDEAAEQVKQYHREGFYVQGGMGMSFDISRYMRRMEIFLADCAGEPDRVKALLDKIAT
                     MLERQVHRYADIGCDGIASGEDWGTQDRLLTSPKMFRELFKPVYMRVCGAAKERGMSF
                     RLHSCGFVRDIIPDWIEAGVHILQFDQPELHNIDFLAEHFGGKVHFECPVDIQTTLQT
                     RDHATIERAARMYIDKLAAPFGGGFIAGYYGSNEALGLDPEYQETASRTYMEYGQNPW
                     KRTAVPA"
     gene            complement(1239..2147)
                     /locus_tag="ENN56_03830"
     CDS             complement(1239..2147)
                     /locus_tag="ENN56_03830"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDS74651.1"
                     /translation="MSDTEHAVTSRYTNPGAEKWLDNPIACLDHGFVMLKDYMGGDES
                     IVQAARVSYGRGTKHSSSDKALIDYLLRHRHTTPFEMVEFKFLAKMPIFVARQWIRHR
                     TASVNEMSARYSVMEDDFFVPAPEELRFQSTTNRQGGSADEVPPELAQKVADILRRGT
                     ERTYAEYSEMIEQDIARELARIHLPISLYTQWYWKIDLHNLMHFLSLRMEAHAQREIR
                     VFADAMAQIVRDAVPWSWEAFEEYNLGATYLGKSESEVIGALLRGENVTALPTPIAKG
                     GRRREFEEKLRSFGIDPEKALPPIVE"
     gene            complement(2288..3262)
                     /locus_tag="ENN56_03835"
     CDS             complement(2288..3262)
                     /locus_tag="ENN56_03835"
                     /inference="COORDINATES: protein motif:HMM:PF12706.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS74652.1"
                     /translation="MRISVMQITIWGSRGSLPTPMPLNEYEQKQRTLLEAYRDAGCPP
                     DLKGFLSSREFHERSTYGGNTSCVEVTEGDQQIIFDAGSGLRPLGLKMMGGPCGKGKG
                     DVRLLISHTHWDHLMGFPFFVPAFIPGNTLTFYGCHDHLKERFEYQQVFTHFPVTMDQ
                     MASKKEFVRIEPGEPFTIGPFHIKAITQYHPGDAYGYRVETESGVFVYATDAEYTELS
                     WEDRQRHVEFFRDADVVIFDAAYSLVEAVEKLDWGHSSPYLGVEMANDANVKTLVLFH
                     HDPTTRDAAVSDTLRKAQRFYEQLPNKTSHLTVVSAYDYQTFTLPAES"
     gene            3482..3970
                     /locus_tag="ENN56_03840"
     CDS             3482..3970
                     /locus_tag="ENN56_03840"
                     /inference="COORDINATES: protein motif:HMM:PF07828.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74653.1"
                     /translation="MNNLHRTLHFGALLVMAVTISCTPPPPITDAIVVDARTEHWQSQ
                     IAGAGPNAGVNVKTGDEITLAIDGKITYDRSLHGLRKDTSGPWGVRAPKPEAWCPEAR
                     IGALVWRIGDDGPCLSLDKVSGMTFTADRDGQLQFLVNDIKGGYGDNSGSFRVTVTIQ
                     RR"
     gene            complement(4058..4930)
                     /locus_tag="ENN56_03845"
     CDS             complement(4058..4930)
                     /locus_tag="ENN56_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_229101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS74654.1"
                     /translation="MELRTLGRTGERLSAVGFGGIVVDKMEHREAERWVDEALAAGIN
                     YFDVAPSYGDAETRLGPALYGKRDEVFLACKTTERTRDGASRELEQSLENLRTDRFDL
                     YQAHAMSTLDDVETFFGPNGAGETFLRAREEGKTRYLGFSAHTEEAALAMMERFDFDT
                     ILFPTNYVNWLTGGFGPRVLERARSAGMGILALKAMARQKWPEGTDKSRYSKCWYEPE
                     DDERFAHLALRFAWTQGVTACVPPGAPELWRMAVRLANQGTEPLSDAEMDELRERAVG
                     KTPIFAAIGGATLR"
BASE COUNT          965 a         1553 c         1366 g         1053 t
ORIGIN      
        1 cggcgactcc caccagtacg agcagccctg cgccgatctc accgatgcgc tcaccgtcaa
       61 ctgtcacact cgcttcggat acgcgctgca ctaccgctct catgattcat ctccaacgct
      121 ctaaacacga agcgggacgc agaataccac gtcccgcctc gcagttcgct acccagtcgt
      181 tcttacgctg gtaccgcggt ccgcttccac ggattctgac cgtactccat ataggtgcgc
      241 gacgcggttt cctgatactc cggatcgagc ccgagtgctt cgttcgagcc gtaatagccg
      301 gcgatgaatc caccgccgaa aggtgccgcc agcttgtcga tgtacatccg cgccgcacgc
      361 tcaatcgtcg cgtggtcgcg cgtctgcaag gtcgtctgga tatccaccgg acactcaaag
      421 tgcaccttac cgccgaagtg ttccgcgagg aaatcgatgt tgtgcagctc cggctggtcg
      481 aactggagga tgtgaacgcc cgcctctatc cagtccggga tgatatcgcg cacgaatccg
      541 cacgagtgca gccggaagct catgcctcgt tccttcgccg caccgcaaac acgcatgtac
      601 accggcttga acagttcgcg gaacattttc ggactcgtga gcagtcggtc ctgcgtgccc
      661 caatcctcac ccgacgcgat cccgtcgcag ccgatatcgg cgtaacgatg aacctgtcgt
      721 tcgagcatcg tcgcgatctt gtcaagcagc gccttcacgc gatcgggttc ccctgcgcag
      781 tcggcgagaa aaatctccat tcgccgcatg taccgcgaga tatcgaacga catacccatg
      841 ccgccttgga cgtagaatcc ctcgcgatga tactgtttga cctgctccgc cgcttcgtcg
      901 tagagttccg cgcggtcgat atccggccat tcatacgtct cgagcagctc ccatccctgt
      961 tcgatgacgc cgcgatagac ctcacccttc gtgatgttct cgaggcgacg ccattcgtta
     1021 ccccattcgt cgatcatgtt ccagtagccg tcacgttgtg tccacggctg ctgcttccga
     1081 ttcggcgccg gcttgcgact cacgcgacgg aaatccgatg ggaacccatt tgtcatcgcg
     1141 tacggaatgc gtggcggact ggcgaatgca agactctgct gaacgatttc gcgactggtg
     1201 atggccattg agatactcca aattgtggta tgtgtagctt actcgactat cggtggaagt
     1261 gctttctccg gatcaatgcc gaatgaacgc agcttttcct caaactcacg ccgtctgccg
     1321 cctttggcga tcggcgtagg taacgcggtg acgttctcgc cgcgtaacag cgcaccgatg
     1381 acctcgctct ccgacttgcc caagtacgtt gcgccgagat tgtattcctc gaacgcttcc
     1441 cacgaccacg gcactgcgtc acgcacgatt tgcgccatgg cgtcggcgaa gacgcgaatc
     1501 tcccgttgtg cgtgggcctc cattcgcaaa ctgaggaagt gcatgaggtt gtgcagatcg
     1561 attttccaat accactgcgt atacagcgag atcgggaggt gaattcgcgc aagctcccgc
     1621 gcgatatcct gctcgatcat ttcggaatac tcggcgtagg tccgctccgt gccccgtcgg
     1681 aggatgtccg cgaccttctg cgccaattcc ggcggaactt cgtccgcgct gccgccctgc
     1741 cgattcgtcg tcgactgaaa ccgcaattcc tccggtgccg gcacgaagaa atcgtcttcc
     1801 atgacgctat atcgcgcgct catctcgttt acactcgctg tccggtgccg aatccactgc
     1861 cgcgcgacaa agatcggcat tttcgcaagg aacttgaact cgaccatctc gaaaggcgtc
     1921 gtgtggcgat gtcgaagtag gtagtcgatg agcgccttat cggacgacga gtgcttcgtt
     1981 ccccgaccgt agctcacccg cgccgcttgc acaatcgatt cgtcaccgcc catgtagtcc
     2041 ttgagcatga cgaagccgtg atcgagacag gcaatggggt tgtcgagcca cttttcagcg
     2101 ccgggattcg tataccgcga tgtcacagcg tgctctgtgt cggacatagg ttagcctctg
     2161 tgatctggcc attgtcgtag gttggtaaaa ggaaggaacg cagtcacgcg attcggggca
     2221 agtgttcgag agaacaccta ccgatatccc cgctgaatac tgcgttgaag agccttgcgc
     2281 cgacggccta tgattcagcc ggtagcgtaa acgtctggta gtcgtacgcg ctgacgacgg
     2341 tgaggtgcga cgtcttgttc ggcagttgct cgtagaaacg ctgtgcctta cgcagcgtgt
     2401 cggagaccgc cgcgtctcgc gtcgtcgggt catgatgaaa caacacgagc gtcttcacgt
     2461 tcgcgtcgtt cgccatttcg acaccgaggt acggtgagct atgcccccaa tcgagctttt
     2521 ccaccgcttc gacgagactg tatgcagcgt cgaatatgac gacgtcggca tcgcggaaga
     2581 actctacgtg tcgttggcga tcttcccacg acagttcggt gtattccgca tcggtggcgt
     2641 acacaaacac gccggactcc gtctcgacgc gatacccgta cgcatcgccc ggatgatatt
     2701 gcgtgatcgc cttgatgtgg aacgggccga tggtgaacgg ctcgcccggc tcgatgcgca
     2761 cgaactcttt cttactcgcc atctgatcca tggtgaccgg gaagtgcgtg aacacctgct
     2821 gatattcgaa ccgctccttg agatggtcgt ggcagccgta gaacgtcagc gtgttccccg
     2881 gaatgaacgc gggcacaaag aaggggaatc ccatgaggtg atcccagtgc gtatggctga
     2941 tgagaagccg aacgtcacct tttccttttc cgcatggtcc gcccatcatc ttcaatccga
     3001 gcgggcggag tccactccct gcgtcgaaaa taatctgctg atcgccctcg gttacctcga
     3061 cacacgatgt gtttccgccg tacgttgacc gctcatggaa ctcgcgcgag gaaaggaacc
     3121 ccttcagatc cggcgggcaa ccggcatcgc ggtacgcttc gaggagagtg cgctgcttct
     3181 gttcgtattc gttcagcggc atcggcgttg gcagcgaacc gcgagatccc caaatggtga
     3241 tctgcatcac agaaatcctc atggaatatg gaggatcggt tgagattccg cccgaaagta
     3301 tacagaagcc aaggtcttag atgcaacaca cattgccaat cgcgataata ccgctgataa
     3361 acgccgcgga atcggttgct tacgccgcgg ccattgcttg acgtattcac gcggtgaatc
     3421 tacactcagt accggaaatg atgcactgat acgcaccacg attccgatat cgaggatacc
     3481 aatgaacaat cttcaccgca cactgcactt cggcgcgctc cttgtcatgg ccgtcacgat
     3541 ctcatgtacg ccgccgccac cgatcaccga cgcaatcgtc gtggatgcgc ggacggagca
     3601 ttggcagagc cagattgccg gagccggtcc caatgccggc gtgaacgtga aaaccgggga
     3661 cgaaatcacc ctcgccatcg acgggaaaat cacgtacgac cgcagccttc atgggcttcg
     3721 caaggatacg tccggaccgt ggggcgtacg tgcgccgaag cccgaagcgt ggtgccccga
     3781 agcgcgtatc ggagcgctcg tctggcgaat cggcgatgac ggaccgtgcc tttcactcga
     3841 caaggtttcc ggaatgacct ttaccgccga ccgcgacggg caactccagt tcctcgtgaa
     3901 cgacatcaag ggtggctacg gcgacaactc cggctcgttc cgcgtgactg tcacgattca
     3961 acgccgatag agctgctccg ccgttactcg gctcgatcta ttgcgacgtt gcgctgtcta
     4021 tcgcagcgtt gcgccgccta tcgcagcgtt gcgccgccta tcgcagcgtt gcgccgccta
     4081 tcgcggcgaa gattggcgtt ttcccaacag cgcgctcgcg aagctcgtcc atctcggcat
     4141 cgctgagtgg ctccgtaccc tgattcgcga gccgtaccgc catgcgccag agctccggcg
     4201 ctccgggtgg cacacatgcc gtgactccct gcgtccacgc gaaccgcaac gccagatgcg
     4261 cgaaccgttc gtcatcctcc ggctcgtacc agcatttgga ataccggctc ttgtcggtcc
     4321 cttcaggcca tttctgccgt gccatcgcct tgagcgcgag aatccccata ccggcactcc
     4381 gtgcccgctc aagaacgcgc ggaccaaagc cgccggtgag ccagttgacg taattcgtcg
     4441 ggaacaggat cgtgtcgaaa tcgaaacgct ccatcatggc gagcgcggcc tcctccgtgt
     4501 gcgcggaaaa gccgaggtag cgcgttttcc cttcttcacg agcgcggagg aacgtctcgc
     4561 cggcaccgtt tggaccgaag aatgtctcca cgtcgtcgag tgtgctcatt gcgtgcgcct
     4621 gatacagatc gaatcggtcc gtgcggaggt tctccaatga ctgctccaac tcacgactcg
     4681 ccccgtcacg agttcgctcg gtggttttac acgcgaggaa gacctcgtcg cgcttgccat
     4741 acagcgcggg gccgagtcgt gtttccgcgt cgccgtagct cggtgcaacg tcgaagtagt
     4801 tgatcccggc ggcgagcgct tcgtcgaccc atcgctcggc ctctcggtgt tccatcttgt
     4861 caacaacgat tccaccgaat ccgacggcgc tgagacgttc gccggtgcgt ccaagtgtcc
     4921 gcaattccat gttcgcc
//
LOCUS       DSCC01000312            1838 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043254, whole genome shotgun sequence.
ACCESSION   DSCC01000312 DSCC01000000
VERSION     DSCC01000312.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043254"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..600)
                     /locus_tag="ENN56_03850"
     CDS             complement(<1..600)
                     /locus_tag="ENN56_03850"
                     /inference="COORDINATES: protein motif:HMM:PF11412.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74655.1"
                     /translation="MKQNTRTLLLVALIFTLGAYVGSQVPPPESTPPKPIKLSERFPW
                     SVEPTVATISAGDTLHITAILGVPDQHYVYAERTGVDITPPSGIAVGEIVTDAPKLKY
                     DTFEKRDVPQYDSSATFRIPLIADVSAGGTFEISLTLSTQGCSPTMCYFPTSVERTVL
                     VTVMPSGAAEGALSDFSRATDGTDTGALSGAVNLGNVVAE"
     gene            complement(642..>1838)
                     /locus_tag="ENN56_03855"
     CDS             complement(642..>1838)
                     /locus_tag="ENN56_03855"
                     /inference="COORDINATES: protein motif:HMM:PF04459.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDS74656.1"
                     /translation="LGIHLKGPGFKRCNNHCVFCFIDQNPGGVRRPLRFKDEDFCLSF
                     LYGNYVTLTNTPREDLDRIIEQRLAPIYVSVHATEPELRRELLGNPKAPDILPIMQYL
                     ADGEIIQHAQVVLMPGKNDGEHLDRTVDDLTELYPWVGSICIVPVGVTKHRAKLPELR
                     VHTAEEARALLRWQEQRQERFLGELSERFVYISDEFYLVAGEPIPEMEFYEGFPQVGN
                     GVGMIRQFMDAFEDGLPRLTKRFTDDVNPLRIDLVTATLPARFLLDMIHRVHEVAPGI
                     AITPHAVTNEWYGEGITVSGLLSGRDIVAAVRERGRGEVVLLPPNCLNDDMLFLDDLH
                     AEDAQEQIGKPVVFGTYDLIESIELAYDRVHSAGANSGLLDISRGPARDEAESDDDAP
                     ETMRAV"
BASE COUNT          348 a          523 c          585 g          382 t
ORIGIN      
        1 ctcggcgacg acatttccga gattcaccgc gccgctcagc gcaccggtat cagtgccgtc
       61 agtggcacgc gagaagtcgc taagcgctcc ctcggcggcg cctgatggca tgacggtgac
      121 caatacggtg cgttcgacag aggtcgggaa gtagcacatg gtcggcgaac agccctgcgt
      181 gctcagcgtc agcgagattt cgaacgttcc gcccgcggaa acatcggcga taagggggat
      241 gcgaaaagtc gcggaactgt cgtattgcgg cacgtcgcgc ttctcgaagg tgtcgtactt
      301 cagcttcggc gcgtcggtaa cgatttcacc gacagcgata ccggacggcg gcgtgatgtc
      361 gacacctgtg cgttcggcgt agacgtagtg ttgatcggga acgccgagta ttgccgtgat
      421 atggagcgta tcgcccgccg atatcgtcgc gacggtcggc tcgacactcc acgggaatcg
      481 ttcgctcagt ttgatcggct tcggcggtgt cgattcgggt ggcggcacct gcgagccaac
      541 gtacgcgccg agtgtgaaga tgagcgccac gaggagcagt gttcgagtgt tctgtttcat
      601 ctatttcctc aaatattcgg ggcatcccat acggccgaga atcacaccgc gcgcattgtt
      661 tcgggcgcgt cgtcgtcgga ctcggcttcg tcacgtgctg gtccccgtga gatatccaga
      721 agtccggagt ttgctccggc actatggacg cgatcgtacg caagttcaat gctttcgatc
      781 aggtcatacg ttccaaacac caccggcttg ccgatctgtt cctgtgcgtc ttccgcgtgg
      841 agatcatcga ggaacagcat gtcgtcgttc aggcagttcg gtggcaggag aacaacctcg
      901 ccgcgaccgc gttcgcgcac ggccgcgacg atatcccggc cactgagcaa gccgcttacg
      961 gtgataccct cgccgtacca ttcattcgtg acggcgtgcg gagtaatcgc gatgcccgga
     1021 gcgacctcgt ggacgcggtg gatcatgtcg aggaggaatc gcgcgggcaa cgtcgcggtg
     1081 accagatcga tccgaagcgg atttacgtca tcggtgaatc gcttcgtgag tcgcggcagt
     1141 ccgtcttcga acgcgtccat aaactggcga atcatgccaa cgccgttccc gacctgcggg
     1201 aagccttcgt aaaactccat ctccggtatc ggctcgccgg cgacgaggta gaactcgtcg
     1261 ctgatgtaga cgaagcgttc ggagagctcg ccgaggaacc gctcctgccg ctgttcctgc
     1321 caacgcagca aggctcgcgc ttcctcggcg gtgtggactc ggagttcggg caacttcgcg
     1381 cggtgtttcg tcacgccgac gggcacgata cagatcgagc cgacccatgg atagagctcg
     1441 gtaaggtcgt ccacggtgcg atcgaggtgt tcgccgtcgt tcttgcccgg catgagcacg
     1501 acctgcgcgt gctggatgat ctcgccatct gcaaggtact gcatgatcgg gaggatgtct
     1561 ggcgctttgg gattgccgag gagctcgcga cggagttcgg gttcggtagc gtgcacggat
     1621 acgtagatcg gcgcgaggcg ttgctcgatg atgcgatcga gatcttcccg tggcgtattc
     1681 gtgagggtga cgtagttgcc gtagagaaac gacaggcaaa agtcttcgtc cttgaaccgg
     1741 agcggacgcc gcacgccacc tggattctgg tcgatgaagc agaaaacgca atggttgtta
     1801 caccgtttga atcccggtcc tttcagatgg ataccgag
//
LOCUS       DSCC01000313            3047 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021907, whole genome shotgun sequence.
ACCESSION   DSCC01000313 DSCC01000000
VERSION     DSCC01000313.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021907"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..536
                     /locus_tag="ENN56_03860"
     CDS             <1..536
                     /locus_tag="ENN56_03860"
                     /inference="COORDINATES: protein motif:HMM:PF01926.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HflX family GTPase"
                     /protein_id="HDS74657.1"
                     /translation="VVLTDTVGFIRKLPHHLVASFRSTLSEAREADIVLHVVDASHPN
                     AAEQIAVVREVLDEMDIRPPHTILVMNKIDQIDAADPESTVLPSKLAGGMPHVDISAV
                     TGTGLDRLRDELAACIEAEMVELEVRVPQANGKLISRIHDRGEVLSRSYERNDVILRA
                     RLRRADANMIESELAIP"
     gene            622..1002
                     /locus_tag="ENN56_03865"
     CDS             622..1002
                     /locus_tag="ENN56_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74658.1"
                     /translation="MHATCTVTTVAEYDLLNPQTPTSAIEGYFRKPLATWDPQSLEPG
                     EVDAVKRAFVRSTETRQIEIDREEDVIWVTYTIAIDLDYDQNDLEYFGSSVDEVLEKG
                     VQVSFPLDERVVELVGEEITVKTQ"
     gene            1052..1810
                     /locus_tag="ENN56_03870"
     CDS             1052..1810
                     /locus_tag="ENN56_03870"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HDS74659.1"
                     /translation="MRGMILAAGLGTRMKPLTDRLPKPLLPVAGYPLIAYPLGLLKAA
                     GITDVVVNIHHLGHLIEKQLAGCADEGMNITFSRETSLLETGGGIARAAKLIGDDTFV
                     VLNADAICDIDLREVIEDHHRYNAIATMVLRKNPDTATIPIVEWDSNSRHVVDIRSEL
                     GVKYSYSLPLMFTGIHIFGPDVFDYLIPMRESVIDGFYLPAIRENRHIHGYVFDGYWE
                     DIGTLDRYERARAVVSPTSLQAFQPLNIPTVDLA"
     gene            1807..2844
                     /locus_tag="ENN56_03875"
     CDS             1807..2844
                     /locus_tag="ENN56_03875"
                     /inference="COORDINATES: protein motif:HMM:PF01636.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74660.1"
                     /translation="MSLPGITEEQIAELLTYATGSSSSQPSLLVGGASDRWYARISGE
                     LPKRFGCRTLLVTGVPTTLWSILESFLRNQSFLERLQCPVPRVYGRWPQYGIALIEDF
                     GDIRLVDAISREPKRRNELDENAIDLLVRLHSLPPDPHLSYPARELAFDVEKYQYEFG
                     FHVDTWLIGHHLGVSPTATERRALDGTYRWISETLAAEPFVFTHRDYQSTNLMLRDDG
                     TLGLIDFQDARQGLRQYDLASFIWDSYVSKSDERRYRITQYYRTAAGVTDDPDRFDAL
                     LRIAAIQRKLHDAGAFVYTAAHRGKTEYLVWVSDAIGIATDLMIEFHQCHDTGAMLAE
                     WMERKRQAMRE"
     gene            2841..>3047
                     /locus_tag="ENN56_03880"
     CDS             2841..>3047
                     /locus_tag="ENN56_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74661.1"
                     /translation="MNRTRWLFWDLGDTILNEDPLRFEFYRFLYEALLEERYTGSFED
                     LLSERETSITNGIFATHYAIARERL"
BASE COUNT          735 a          846 c          817 g          649 t
ORIGIN      
        1 ctgtcgtact caccgatacg gtcggattta ttcgaaagct gccgcaccat ctcgtcgcgt
       61 cgtttcgaag cactctgtcg gaagcgcgag aagctgatat cgtgctgcat gtggtcgacg
      121 cgagtcatcc gaacgcggcg gagcagattg ccgttgtgcg cgaagtactc gatgagatgg
      181 acattcggcc gccgcacacg attcttgtga tgaacaaaat tgatcaaatc gatgccgccg
      241 acccggagtc aaccgtgctt ccgtcgaagc tcgccggagg gatgccacac gtcgatatca
      301 gcgcagtaac cggtacggga ctcgatcgcc ttcgcgacga gctcgcggca tgtatcgaag
      361 cggaaatggt agagcttgaa gtgcgcgttc cgcaggcgaa tggcaaactc atatcgagaa
      421 tacacgatcg cggagaagtc ctgtcacgct catacgaacg caatgatgtc attctccgcg
      481 ctcggcttcg ccgcgccgat gcaaatatga ttgaaagcga actggcaata ccgtagagcg
      541 gatagaaagc gaaaagcgcg acggattgcg aggcgcaata ccgaatccga tattctcatc
      601 acgcacgata caggaatctc gatgcacgca acgtgtaccg tcaccacggt cgcagaatac
      661 gatctcctca atccgcagac gccgacgagc gcaatcgaag ggtatttccg aaaaccgctc
      721 gcgacctggg acccgcaaag ccttgaaccc ggtgaagttg acgcggtaaa gcgggcgttt
      781 gtccggagta ccgaaacgag gcagatcgag atcgaccgtg aagaagacgt catctgggtt
      841 acctatacaa tcgcgatcga cttggattac gatcaaaacg atcttgaata cttcggttct
      901 tcagtcgatg aagtactgga aaaaggcgtg caagtgagtt tcccactcga cgaacgagtc
      961 gtcgaactcg taggcgagga aatcacggta aagacacaat gattcccgtg gaccgacaga
     1021 ttgtggaaca cggcacggag gcacggcgca catgcgtgga atgatacttg cggcgggact
     1081 cggcacgcgg atgaagccgt tgacggatag gttgccaaag ccgctgcttc cggttgcagg
     1141 ttacccactc atcgcgtatc cgctcgggtt attgaaggcc gctggaataa ccgacgtcgt
     1201 cgtaaacatc catcacctcg gtcatctcat tgaaaagcaa ctcgccggat gcgccgatga
     1261 agggatgaat atcacgtttt cacgcgaaac gtcgttgctt gagacgggcg ggggaatcgc
     1321 gcgtgcggcg aaactcatcg gtgacgacac gttcgtcgtt ttgaacgccg atgccatttg
     1381 cgacattgac ctgcgcgaag ttatcgaaga tcatcaccga tataacgcaa tcgctacgat
     1441 ggttctccgc aaaaatcccg ataccgcaac gattccgatt gtagaatggg attcgaacag
     1501 tcggcacgtt gttgacattc gatccgaact cggtgtgaaa tactcctaca gcctcccgtt
     1561 gatgttcacg ggcatccaca tcttcggtcc tgacgtattc gactacctca ttccgatgcg
     1621 tgagtcggta attgacggct tctacctgcc ggcgatacga gaaaatcgcc acatacacgg
     1681 gtacgtgttc gacggatact gggaagatat cggcacactt gaccgctacg aacgtgcgcg
     1741 agccgttgtc tcaccgacgt cgcttcaagc atttcaacca ctcaatatcc caacggttga
     1801 tctcgcgtga gcctacccgg tattaccgaa gaacagatcg ccgagctcct cacatatgca
     1861 accggcagct ccagcagtca gccgtcgttg ctcgtcggag gcgcgtcgga tcgctggtac
     1921 gcgagaatat ccggcgaact tcccaaacga tttggttgtc ggacgctcct cgtgaccggc
     1981 gtgccaacaa ccttgtggag cattctcgaa agctttctgc gaaatcagtc ctttctcgag
     2041 cgtctccagt gcccggtgcc acgtgtatac gggcgatggc ctcagtacgg tatcgcgttg
     2101 atcgaggatt tcggagatat acgcctcgtt gacgcgattt cgcgcgagcc aaaacgccga
     2161 aacgaactcg atgagaacgc aatcgatctc cttgtgcgac ttcattcgtt accgcccgat
     2221 ccacatctct cgtatccagc gcgcgagctt gcgttcgacg tcgaaaaata ccagtacgag
     2281 ttcggattcc acgtcgatac gtggctcatc ggccatcact tgggcgtttc accgactgcg
     2341 accgaacgtc gcgcactcga cgggacctat cggtggatta gcgagacact ggcagcggaa
     2401 ccgttcgtat ttacccatcg cgactatcag agcacgaatc tcatgctacg agatgacggg
     2461 acattaggac ttattgactt tcaagacgca cgccagggat tacgacaata cgacctcgca
     2521 tcgttcatct gggattcata cgtgtcaaaa tccgatgaac gtcgatacag gattactcaa
     2581 tactaccgaa cagccgcagg tgtgacggac gatcctgacc ggttcgacgc gctactccgc
     2641 atcgctgcga tacagcgcaa gcttcatgac gccggagcgt ttgtctatac cgccgcccat
     2701 cgtgggaaaa ctgagtatct cgtgtgggtt tccgacgcga tcggtatcgc gaccgatctc
     2761 atgatcgagt tccaccaatg tcacgacact ggagcgatgc ttgccgagtg gatggagcga
     2821 aaacgacagg ctatgagaga atgaatcgga cacgttggct tttctgggac ctcggcgata
     2881 cgatactcaa cgaggatccg ctccgattcg agttctatcg gtttctctat gaggcacttc
     2941 tcgaagagcg gtacaccgga tcgtttgaag acctcctttc cgagcgcgaa acctctatca
     3001 caaacggcat cttcgcaaca cattatgcca tcgcacgcga acgtctc
//
LOCUS       DSCC01000314            4445 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1013169, whole genome shotgun sequence.
ACCESSION   DSCC01000314 DSCC01000000
VERSION     DSCC01000314.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1013169"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..173)
                     /locus_tag="ENN56_03885"
     CDS             complement(<1..173)
                     /locus_tag="ENN56_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HDS74662.1"
                     /translation="MRHETIMLAHGSGGRRMHDLIRTTIGHILIRPEHTRYSDSAVLG
                     ISNGRVAFTTDTYV"
     gene            complement(208..843)
                     /locus_tag="ENN56_03890"
     CDS             complement(208..843)
                     /locus_tag="ENN56_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74663.1"
                     /translation="MHTIISLWRSALYRVVEIYDTRRGSFRSFFPKLFVFFVALNIAC
                     YWLAMFTAFPELTSGEAGWHYFKVQFPVGVLGALFDSVSFFATVWIVRRALNTHSATE
                     YVAHLSVDLAIAMLATLWVVFVFTFSGWIINLLAQSSQSYAERSARYNAMLVDAAANP
                     IDNVRNIYFGLVMGLSSALPTVLHLSLFARSTVVAFGKRILLPVVDRREFR"
     gene            complement(940..2040)
                     /gene="hypD"
                     /locus_tag="ENN56_03895"
     CDS             complement(940..2040)
                     /gene="hypD"
                     /locus_tag="ENN56_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDS74664.1"
                     /translation="MKYLDEYRDPSLCRSILREIHRVRIDKPVTIMEVCGTHTMAVAR
                     YGIRRLLPDTVRLISGPGCPVCVTPNRYLDYACALAKTRGVTIVTFGDMLRVPGSRSS
                     LEHEQARGADIRVVFSPLETLAFAERNPKRNFVFLGVGFETTVPTIASLIIQAAERGV
                     RNVSVLAAPKTMPPPMRALAADSNVRVDGFLCPAHVSAIIGTKLYDGIVRDFGIPCVV
                     AGFEPVDILRSIHLILERLRDESPGVDIEYSRVVRPEGNPRAQRIVERVFESVEAEWR
                     GLGVIPDSGLAIRSEFQQFDAASRFPLDIPPPTEHAGCLCGDVLRAIASPDDCSLFGS
                     TCTPHSPVGPCMVSTEGTCAAAYAYERHARHV"
     gene            complement(2042..2275)
                     /locus_tag="ENN56_03900"
     CDS             complement(2042..2275)
                     /locus_tag="ENN56_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HDS74665.1"
                     /translation="MCLGVPMQVIELKDDTAMVELGGVRREISMALVEDCAIGDYVLV
                     HAGFAISKLDATDAEGTIGLLREKYGASEGIES"
     gene            complement(2287..>4445)
                     /gene="hypF"
                     /locus_tag="ENN56_03905"
     CDS             complement(2287..>4445)
                     /gene="hypF"
                     /locus_tag="ENN56_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDS74666.1"
                     /translation="VEIEVEGPETSRHAFLTALRKPPPLARIDRQTVRDASPRNGESR
                     DFSILPSNDDIADQVTPVTPDAAICADCLRELFDPNDRRYRYPFITCTHCGPRFSIVT
                     GVPYDRPQTTMASFPLCSECRAEYEDPTDRRFHAQPIACPVCGPHVSLFNSEGVSLQG
                     ADPIEAVVDTLEAGGIVAIKGIGGYHLACDAFNVDSCTELRKRKIREEKPFAVMARNL
                     SVAGRIGNLSAEANRLLQSPQSPIVIVPRRCDSSALSVIAPNNRTIGLLLPYSPIHHL
                     LFHGARYDALVMTSGNCSDEPICFEDDDALVRLRGIADCFLVHNRPIHVRVDDSVSRM
                     TKCGPVPVRRSRGYAPAPICLKTSLNYPVLAVGAMLKHTFALGSGSRAYVSQHIGDLE
                     NAATWKSFVDGVERYKKLTGIDPELVAHDLHPDYLSTQYAERLGLPMIPVQHHHAHIA
                     SCLADNGCDGPAIGIAFDGTGYGDDGTLWGGEWLVVDGPRYTRAASLRHIALPGGESG
                     IRQPWRIGAGMLDTIYANDWRGRGLPIERFMENDEWEQLRRYFGDMTYTPTTSAVGRL
                     FDGVAAIAGVRGSITYEGQAAIEFEQAHDRTVDGAHPFDTTRNGDRYELDWSSTVRAV
                     IDDLCRNRNIGAVSMRFHRGLAVAVADVTEQIARDVRIRVCALSGGVFQNALLLELLV
                     PLLRDRGLRVLIHREVPPNDGGISLGQLVIAGGKAI"
BASE COUNT         1035 a         1258 c         1229 g          923 t
ORIGIN      
        1 acataggtgt cggtcgtaaa cgccacccgt ccattggaaa tgccaagcac cgcggagtcg
       61 gaataccgag tatgttccgg tcggatgagg atgtgtccga ttgtcgtgcg aataaggtcg
      121 tgcatacgtc gaccacccga cccatgggca agcatgatcg tttcgtggcg catatccgac
      181 ctttctaata cattgttggc gtattactta gcgaaattct ctccgatcga cgacgggcaa
      241 caaaatacgt tttccgaacg cgacaacagt cgatcgagca aataacgaaa gatgtagcac
      301 tgtcggcaaa gcacttgata aacccataac aagtccaaag tagatgttcc gcacgttgtc
      361 tatcggattc gctgctgcat caacgagcat cgcgttgtag cgcgcgctac gctccgcgta
      421 actctgagaa ctctgtgcca gcagattgat aatccatccc gagaacgtga acacaaatac
      481 aacccacaga gttgcaagca tcgcgatcgc caagtcgacg gaaagatggg cgacgtactc
      541 agttgcgctg tgagtattca gtgcgcgtcg cacgatccac acggtggcaa agaacgagac
      601 actgtcgaat agagcaccta atacaccgac agggaactgc actttgaagt agtgccaacc
      661 cgcttcaccg gaggtaagct cgggaaatgc ggtaaacatc gcgagccaat agcacgcaat
      721 attcaacgcg acgaagaaca cgaaaagttt cgggaagaac gagcggaatg aaccgcgtcg
      781 cgtgtcgtat atctcgacta cacggtagag agcgcttcgc caaagcgaga taatcgtgtg
      841 catctatgaa atccgtcctg tgtgcatcca tgcaattcgc ggcgcgaaaa aggtacgcgt
      901 gcacgactaa agcgcaaacc acaacactgc gtaccgttct tatacatgcc gagcatgccg
      961 ttcgtacgcg taggcagccg cacaggttcc ctcggtagac accatacacg gaccgacggg
     1021 agagtgggga gtgcacgtcg aaccaaagag ggagcaatca tccggcgagg cgattgcgcg
     1081 tagaacgtcg ccgcacaggc atccggcatg ctccgttggc ggagggatat cgagtgggaa
     1141 tcgagaagcg gcatcgaact gctgaaactc agaccggatt gcgagtcccg agtctgggat
     1201 cacgccaagc ccacgccatt ctgcctcgac ggattcgaac actcgttcca cgatacgttg
     1261 agcgcgcgga ttcccctctg gacgcactac ccgcgagtat tcgatatcaa cacccggtga
     1321 ttcatcacgc aatcgctcga gtatcaggtg tattgaacgg aggatgtcga cgggttcgaa
     1381 tccggcgacg acgcagggaa ttccgaaatc gcgaacgatt ccgtcgtaga gcttcgttcc
     1441 aatgatcgcg cttacgtgag ccggacagag aaacccatcg acacggacgt tcgagtcggc
     1501 ggcaagtgcg cgcatcggcg gcggcatcgt tttgggtgct gcgagaacgc tcacattcct
     1561 cacgccccgt tcagccgcct gaatgattaa cgatgcgatc gtcggcacgg tagtttcaaa
     1621 tccgactccg aggaacacga aattccgttt ggggttacgt tcagcgaacg cgagcgtttc
     1681 gagtggggag aacacgactc gaatatcggc accgcgtgcc tgctcatgtt cgagacttga
     1741 tcgcgaaccg ggcacgcgta acatatcgcc gaatgtcacg atggtgacgc cgcgagtctt
     1801 cgcgagtgcg catgcgtagt cgagataccg gtttggcgtc acgcataccg gacacccggg
     1861 tccacttatg agtcgcacgg tgtcaggaag caagcgccga ataccatacc gtgcgacggc
     1921 catggtgtgt gtgccacaca cctccatgat cgtaacgggt ttatcaatac ggacgcgatg
     1981 gatttcgcgg agtatcgatc ggcagagtga gggatcacgg tattcgtcga ggtatttcat
     2041 gtcagctctc gatgccttcg gatgctccgt atttctcgcg aagcaaaccg atagttccct
     2101 cagcgtccgt cgcgtctaac ttactgattg cgaatccagc gtgaaccaag acgtagtccc
     2161 ctatagcaca gtcttctacg agcgccatac tgatttcgcg gcgaactcct ccgagttcga
     2221 ccatcgctgt atcgtctttc aattctatca cttgcatcgg aacaccaaga cacattttga
     2281 cctctcctat atcgctttgc cgcctgcgat caccaattgc ccgagactga ttccaccgtc
     2341 gttcggcggc acctcgcgat gaatcagaac gcgtaatccg cgatcgcgta ataacggaac
     2401 caagagttcc agcaacaacg cgttctgaaa tacccctccg cttagcgcgc aaacacgaat
     2461 tcgcacgtct cgcgctattt gttcagtcac atccgcaaca gcgactgcaa gccctcgatg
     2521 gaaacgcatg gacaccgcac cgatgttccg attcctgcag agatcgtcta ttacagcgcg
     2581 aactgtggag ctccagtcga gttcataccg gtcgccgttt cgcgttgtgt cgaacggatg
     2641 cgcgccatca actgtgcggt catgggcttg ctcgaactcg atcgcggctt gtccctcgta
     2701 cgtgatcgat ccgcgaacgc cggcaatcgc agcaactccg tcgaagaggc gaccgacagc
     2761 cgacgtcgtc ggtgtgtagg tcatgtctcc aaaataccgc cgtagttgct cccactcgtc
     2821 attttccatg aaccgttcga ttggaagacc acgtccgcgc caatcgttag cgtaaatggt
     2881 atcgagcatt ccggcgccga tgcgccacgg ttgacgaatc ccgctttctc ctccaggtaa
     2941 cgcgatatgc cgaaggctcg cggcacgggt ataccggggg ccgtctacga ccaaccactc
     3001 gccgccccac agcgtcccat cgtccccgta accggtacca tcaaatgcga taccgatcgc
     3061 cggaccgtcg catccgttat cggcgaggca actcgcgatg tgtgcgtgat ggtgttgcac
     3121 tgggatcatg ggtagtccga gtcgttcggc gtattgcgtt gagagataat caggatgaag
     3181 gtcatgtgcg acgagctcgg gatcgattcc ggtcagcttt ttgtaccgtt caacaccgtc
     3241 gacaaacgat ttccatgtcg cggcgttttc caggtcaccg atgtgttgac tcacgtacgc
     3301 gcgactaccg gagccaagtg caaaggtatg cttcaacatc gcgccgacag ccagaacagg
     3361 atagttgagc gatgtcttta ggcagatcgg tgccggtgcg tatccgcgtg agcgacgtac
     3421 cggcacgggt ccgcacttcg tcattcggga aacactgtcg tcgacgcgaa catgtattgg
     3481 ccgattgtga acaagaaagc agtcggcgat accgcgtaat cgcacaagcg cgtcgtcgtc
     3541 ttcgaagcag atcggctcgt cgctacagtt cccgctcgtc atgaccagag cgtcgtaccg
     3601 agcaccgtga aacaagagat ggtggatcgg gctgtacggg agcaggaggc caatcgtgcg
     3661 attgttcgga gcaatgaccg atagagcgct cgagtcgcat cgtcttggca cgatcacgat
     3721 cggtgattgc ggcgactgca atagccggtt cgcttcagcg gacaggttac cgatccgacc
     3781 agcaacggaa agattacgcg ccataacggc aaacggcttt tcttcccgta tcttcctctt
     3841 acgcaattca gtacacgaat caacgttgaa tgcgtcacac gcaaggtgat aaccaccaat
     3901 cccctttatg gcgacgatac caccggcttc gagcgtatca acaacagctt cgatcgggtc
     3961 tgctccctgt agtgatacgc cttccgaatt gaacagcgat acgtggggtc cgcaaaccgg
     4021 acacgctatc ggctgcgcgt ggaatcgccg atcggtaggg tcttcgtact ccgcgcggca
     4081 ctcggaacag agcgggaagc tcgccatcgt cgtctgcggc ctgtcgtaag gaacgcccgt
     4141 cacgatggaa aaacgcggac cacagtgtgt gcatgtgata aacgggtatc ggtaccgccg
     4201 gtcattggga tcgaagagct cgcgcagaca gtccgcacag attgctgcgt ccggtgttac
     4261 cggtgtcact tggtcggcga tgtcgtcatt gctgggaagt atcgaaaaat cgcgcgattc
     4321 gccgttacgt ggcgaagcat ctcgaacggt ttgccggtca atgcgcgcaa ggggcggtgg
     4381 tttgcgaagt gcagtgagaa acgcgtgacg cgatgtctcc ggtccttcga cctcgatttc
     4441 cacac
//
LOCUS       DSCC01000315            2285 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032089, whole genome shotgun sequence.
ACCESSION   DSCC01000315 DSCC01000000
VERSION     DSCC01000315.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032089"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            103..1407
                     /locus_tag="ENN56_03910"
     CDS             103..1407
                     /locus_tag="ENN56_03910"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDS74667.1"
                     /translation="MWNEIPTIATIAQYVDQVIELKGWLYNKRSSGKLQFLQVRDGTG
                     VMQCVVFKGNVAPEIFEAAAEARQEASIIVCGSIRKDDRSPIGFEMDVTDLEVLHNPD
                     AEYPITPKEHGTAFLMENRHLWIRSSRQQAILRVRAALVKQIRDYFDSNGFMLFDAPI
                     FTPSACEGTTTLFETPYFDDEKAYLTQSGQLYEEAGALAFGRVYSFGPTFRAEKSKTR
                     RHLTEFWMVEPEVAYMGLEGDMDLAEDFVANIVQGVLASCRAHLEHLERDIEALAKVQ
                     KPFPRISYSEAVDIIHAAGKDFQWGDDFGAEEETIISTAYDRPVMIHRYPSAVKAFYM
                     KRDPADEKVALCVDMIAPEGVGEIIGGGQREDDLEKLRGRVTEHGLREEDFDWYLDLR
                     RYGSVPHAGFGLGIERTLGWLCGLPHVRETIPFPRLMQRVRP"
     gene            1476..>2285
                     /locus_tag="ENN56_03915"
     CDS             1476..>2285
                     /locus_tag="ENN56_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDS74668.1"
                     /translation="MVNTSRIEYDFKTLEPKWRAWWDENGVNTANDDDPRPKYYCLDM
                     FPYPSGTGLHVGHWRGYVLSDVWSRYQRLLGRNVLHPMGWDAFGLPAENYAIANKIHP
                     RIATERNVATFKRQLAEIGAMYDWSREVNTSDADYYRWTQWIFLQLYERGLAYRKTSP
                     VNWCPSCQVGLANEEVVGGNCERCGTSVTKRDIEQWFLKITEYAERLLNDLDTLDWPE
                     RVVTMQRNWIGKSVGARVRFDAIRLRDEKSFPLDVFTTRPDTLFGATYMVLA"
BASE COUNT          530 a          640 c          658 g          457 t
ORIGIN      
        1 cgaaagtaca cgtgacacaa tcattcgcgc gcggaacacg tgtgcgaatg atgggaaacg
       61 actgttaaca acggtttttg ctcaggcaac gcgaggtctc ccatgtggaa tgaaatcccg
      121 acaattgcga cgattgctca gtatgtcgat caggtgatcg aattgaaagg ctggctttac
      181 aacaagcgtt cgtcgggaaa gctccagttt cttcaggtgc gcgacggcac cggcgtaatg
      241 cagtgcgtcg tgttcaaggg aaatgttgca cccgagatct tcgaggctgc tgcggaagcg
      301 cgacaggaag cgtcgatcat cgtgtgcggg agtatccgca aggacgaccg ttcgccaatc
      361 ggcttcgaga tggacgtaac cgacctcgaa gtgcttcaca atcctgacgc tgaatacccc
      421 atcacgccga aggagcatgg aaccgcattt ctcatggaga accgtcacct gtggattcgt
      481 tcatcgcgtc agcaggcgat tctcagagtg cgtgcagcgc tcgtcaaaca gatacgcgac
      541 tacttcgaca gcaacggttt catgctgttc gatgcgccga tctttacacc gtctgcatgt
      601 gaaggcacaa cgacgctctt cgaaactccg tatttcgacg acgaaaaggc ctatctgacg
      661 caatccggac agctttacga ggaagccgga gcacttgcat tcggccgcgt gtacagcttc
      721 ggaccgacat tccgcgccga aaaatcaaaa acgcgccgtc atctgaccga gttctggatg
      781 gttgagccgg aggtcgcgta catgggcctc gaaggcgaca tggaccttgc cgaagacttc
      841 gtcgcgaata tcgttcaagg tgtcttggcc agttgccgag cacacctcga gcatcttgag
      901 cgcgatattg aagcgctcgc gaaggtgcag aagccattcc cacgcatatc ctacagcgaa
      961 gcagtcgaca tcatccacgc tgccggcaag gattttcagt ggggcgacga cttcggtgcc
     1021 gaagaggaaa caatcatttc cacagcatac gaccgaccgg taatgattca ccgctatcca
     1081 tccgccgtga aagcgttcta catgaaacgc gacccggccg atgagaaggt tgcactctgc
     1141 gtcgatatga tcgcgccgga gggcgtcggt gagattatcg gcggaggcca gcgcgaagac
     1201 gacctcgaaa agttgcgcgg tcgcgtcacc gagcacggcc ttcgcgaaga agatttcgac
     1261 tggtatctcg atctgcggag gtacggttcg gtgccccacg ccggtttcgg cctgggcatc
     1321 gagcgcacgc ttggctggct gtgcggtctt ccgcacgtgc gagaaacgat tccgttcccg
     1381 cggctgatgc aacgagtccg tccgtaaatc gatcgatacc acagtaagcc gcatcgtgtt
     1441 tcggtgtggc ggacagttcc gaggtactat cgaaaatggt aaatacatct cgtatcgaat
     1501 acgatttcaa aacattggaa ccgaaatggc gggcgtggtg ggacgaaaac ggcgtcaaca
     1561 ccgcaaacga cgacgatccg cgtccgaagt actactgcct cgacatgttc ccgtatccct
     1621 cgggaacggg tcttcatgtc ggccactggc gcggatatgt gctgagcgat gtctggtcgc
     1681 gctatcaacg gttgttaggc aggaacgtgc tccatcccat gggttgggac gcgttcggtc
     1741 taccggctga aaactatgcc atcgcgaaca agattcatcc gcgcatcgcc accgagcgca
     1801 acgtcgcgac attcaaacgg cagcttgccg aaatcggcgc gatgtacgac tggtcgcggg
     1861 aagtcaatac aagcgacgcg gactactacc ggtggacgca gtggatcttc ctccagctct
     1921 acgaacgggg acttgcgtat cggaaaacat caccggtaaa ctggtgcccg agctgtcagg
     1981 tcggcctcgc aaacgaggaa gtcgtcggcg gcaactgcga acgctgcggt acatccgtaa
     2041 cgaaacgtga tattgagcag tggttcctca agatcaccga gtacgccgaa cgcctcctga
     2101 acgacctcga tacgctcgac tggcccgaac gcgtcgtaac gatgcagcgg aactggatcg
     2161 gcaaatccgt aggcgcgcgc gtccgcttcg acgcgattcg gttacgcgac gaaaagtcgt
     2221 tcccgcttga cgtattcacc acgcgccccg acacgttatt cggtgcgaca tacatggtgc
     2281 tggcg
//
LOCUS       DSCC01000316            1542 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054811, whole genome shotgun sequence.
ACCESSION   DSCC01000316 DSCC01000000
VERSION     DSCC01000316.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054811"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..502
                     /locus_tag="ENN56_03920"
     CDS             <1..502
                     /locus_tag="ENN56_03920"
                     /inference="COORDINATES: protein motif:HMM:PF06826.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74669.1"
                     /translation="RFSVGAVVAVFIGGLALPVGPYVRLSLGVTGGALFAGLMFGRSA
                     RIQIPRGAAFFLKELGLGLFLAGVGMSVGKQGPIAAADLILVPGVLAVIAIPAVLAAF
                     LLRRAQLIGPYVEGGVSGAITSTPALIVATRMDETSEPATTYAGIYLFALLTSVAVAQ
                     IIALFG"
     gene            524..>1542
                     /locus_tag="ENN56_03925"
     CDS             524..>1542
                     /locus_tag="ENN56_03925"
                     /inference="COORDINATES: protein motif:HMM:PF06826.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74670.1"
                     /translation="MMIDWTFEILSSTSVGALFLILLTGLALGQVRVFGLSLGSSGVL
                     FTALIAGHFGLLVPSIYGSLGVIFFVYAVGLQAGPRFIHTVRTSGGALIRLGLLLIGS
                     GAATAIIIGMLFDLSPGIIVGAFAGAMTSTPALAAGLDAFRSVSPQEMALVTVSYGLA
                     YPLGIIGVVVPIQVLPKLFRSRLGTDSTATDAQDALANAAFLVENPNIVGRTVDEANL
                     WDFADISLSRILHDGEVNVVLGSTLLSEGDVVLAVGTSEELAKLELLVGPRTVPHLLL
                     SEGSQQIETRTISVGQHAEQDLSTSALSSRYGVSITRLLRLNIALPVGRGMHLEPGDR
                     IHVVG"
BASE COUNT          239 a          471 c          493 g          339 t
ORIGIN      
        1 gcgattcagt gtcggcgcgg tcgttgccgt tttcatcggc ggacttgccc tgccggtcgg
       61 accctacgtg cggctctcgc ttggcgtgac cggcggcgcg ctgttcgcgg gactgatgtt
      121 cgggcgttcg gcgcggattc agataccccg tggcgcggcg ttcttcctga aggaactcgg
      181 actcggcctg ttccttgccg gtgtgggcat gagcgtgggc aagcagggac cgatcgccgc
      241 tgcggatctc attcttgtgc cgggagtgct cgcggtgatc gcaattcccg ccgtgctggc
      301 tgcgtttctc ctccgtcgcg cgcaacttat cggcccgtat gtggagggcg gcgtgagcgg
      361 cgcgataaca agcacaccgg cgctcattgt tgcgacccgc atggacgaga cgagcgaacc
      421 cgcgacgacg tacgccggta tctatctgtt cgcattgctg accagtgtcg cggttgcgca
      481 aattatcgcg ttgtttggct aactgacgac gccattgtgg tgaatgatga tcgattggac
      541 gtttgaaatc ctctcctcga caagtgtcgg agcactgttc ctcattctgc tgacgggact
      601 cgcgctcgga caggtgcgcg tattcggcct gagcctcggc tcgtcgggtg tgttgtttac
      661 cgcgctgatc gcggggcatt tcgggcttct tgttccgtcg atctacggct cgctgggcgt
      721 catcttcttc gtctacgccg tcgggttgca ggccggaccg cggtttatcc acaccgtccg
      781 gacaagcggc ggcgcgctga tccgcctcgg actgcttctg atcggatccg gcgcagcaac
      841 ggcgatcatt atcggtatgc tctttgatct atcgccgggc atcatcgtgg gggcgtttgc
      901 cggtgcgatg accagcaccc ccgcgcttgc cgccggcctc gacgcgtttc ggtccgtctc
      961 gccgcaggaa atggcgctcg tgacagtgag ttacggactg gcgtacccac tcggtatcat
     1021 cggtgtcgtc gttccaattc aagtacttcc gaagctcttc aggtcgcgcc ttgggaccga
     1081 ctcgactgcc accgacgcgc aggatgccct cgcgaacgcc gcatttctcg tggagaaccc
     1141 gaatatcgtc ggccgcaccg tcgacgaagc gaatctgtgg gatttcgctg acatcagcct
     1201 ctcgcggatc ctccacgacg gcgaggtgaa cgtcgtgctc ggttcgacgc tcctcagcga
     1261 gggcgacgtc gtgctggcag tgggaacgtc ggaagagctt gcgaagctcg aactcctggt
     1321 cgggccgcga acagtgccgc acctgttgct cagcgaaggt tcgcaacaga tcgaaacgcg
     1381 gaccatctcc gttgggcaac acgcagaaca ggatctgtcc acatccgcac tgagcagtcg
     1441 atacggtgtg agcatcacgc ggttgctgcg attgaacatc gcgctaccgg tcgggcgcgg
     1501 tatgcacctc gagccgggcg accgtatcca cgtcgtcggt ga
//
LOCUS       DSCC01000317            1563 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053840, whole genome shotgun sequence.
ACCESSION   DSCC01000317 DSCC01000000
VERSION     DSCC01000317.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053840"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="ENN56_03930"
     CDS             complement(<1..1373)
                     /locus_tag="ENN56_03930"
                     /inference="COORDINATES: protein motif:HMM:PF05089.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74671.1"
                     /translation="MILTRNGQPVATIVLDQNASDLERHAVAELNGYTQRMSGGVLPV
                     VNERVDGAEVLIGAVARRVMGNDAPQGDSFAIRCDESGNALLIAGGSDRATLWAVYAF
                     LEDVLGVGFFRDGERVPSQPTIDVPALDIVETPRFSERFEGNGCIYTYSTSSWGWDEW
                     RAEVDWRAKRRVTEIWPLNVGGEIHAEIHDAWGITQPPKTRGSGDSKNRAVKKSLHER
                     IRDYSRSLGMRAPIILSASFSKDYFAAHPEIRTILLKWSELEPYRIIHPADPLFKKYL
                     VEYIRRYAERYGTDHLYIAEFTSESRVLEGADNIQDVREMFVQAVSDAIREADPDGVW
                     LPSTWSFDITPEDPSRPWEVAWTPEHVYRYLDAISVPFIVHDLWSEGSKKYQRLDWFK
                     GHPWGFGVLHSFGAGGYIHGDVQELIELSHDLLTNSRAKNCVMYGAQSEQIDFNGFYY
                     ELVARLS"
BASE COUNT          314 a          499 c          434 g          316 t
ORIGIN      
        1 cacgacagcc gcgcgacgag ctcgtagtag aagccgttga agtcgatctg ttcggattgc
       61 gcgccgtaca tcacacagtt ctttgcgcgt gaattcgtca ggagatcatg cgaaagttcg
      121 ataagctctt gcacgtcgcc gtggatgtat ccacccgcac cgaagctgtg cagcacgccg
      181 aagccccacg gatgaccctt gaaccagtcc agccgctgat acttcttcga accctcggac
      241 cagaggtcgt gcacaatgaa cggcacgctg atcgcatcga gataccggta gacgtgttcg
      301 ggcgtccacg ccacttccca tggccgactc ggatcctccg gcgtgatgtc gaaagaccac
      361 gtcgaaggca gccagacacc gtccggatcg gcctcgcgga ttgcatcgga gaccgcctgt
      421 acgaacatct cgcgcacgtc ttggatgttg tccgcgcctt cgagcacgcg gctttcactg
      481 gtgaattcgg cgatgtagag gtgatccgta ccgtaccgct cggcgtagcg tcggatgtac
      541 tcgacgaggt actttttgaa cagcggatca gcagggtgaa tgatgcggta aggctccaat
      601 tcgctccact tgagcagaat cgtccggatt tccggatgcg ccgcgaaata gtctttcgag
      661 aacgacgcgc tgaggatgat cggcgcgcgc attcccagcg atcgcgagta atcacgaatc
      721 cgctcgtgca acgacttctt cacggcgcga ttctttgaat cgccgctacc gcgcgttttc
      781 ggcggttgcg tgatgcccca tgcgtcgtgg atttctgcgt gaatctcgcc gccgacattc
      841 agtggccaaa tttccgtcac acgcctcttc gcgcgccaat cgacctctgc gcgccactcg
      901 tcccaacccc acgacgaggt gctgtacgtg tagatgcacc cattcccctc gaatcgctcg
      961 gaaaaccgcg gcgtctcgac gatatcgagc gcgggtacgt caatcgtcgg ctgcgacggt
     1021 acgcgttcgc cgtcacgaaa gaagccgact cccaacacgt cttcgagaaa cgcgtacacc
     1081 gcccagagcg tcgcccgatc cgatccgccc gcgatgagta gcgcattccc cgactcgtcg
     1141 caccggatcg cgaacgaatc cccctgcggc gcgtcgtttc ccatcacccg tcgcgcgacc
     1201 gcaccgatca gcacctccgc gccatctacc cgttcattta ccacgggaag gacgccgccg
     1261 gacattcgct gcgtataccc attgagctcg gcgaccgcgt gtcgctcgag gtccgacgcg
     1321 ttctggtcga gaacaatcgt tgcgaccggt tgcccgtttc gtgtcagtat catcgttccg
     1381 cctcctcgca gaaaacgttg tcgtgccgaa taaccgttca accgcgacga ggggcaatag
     1441 accgggggtc gccacgggtg tgacaattcg ctaccattcc atgccgatga ttttgcatct
     1501 gaaaaggaga aatgatcgat gaaacggctg ttgacgggaa cttacgcgaa attcgcgctc
     1561 gcg
//
LOCUS       DSCC01000318            2009 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038239, whole genome shotgun sequence.
ACCESSION   DSCC01000318 DSCC01000000
VERSION     DSCC01000318.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038239"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="ENN56_03935"
     CDS             complement(<1..1435)
                     /locus_tag="ENN56_03935"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDS74672.1"
                     /translation="MHSTRHLRILIISAVGFVLAGYIVYPSIAVVFEAFRSVDGSFTV
                     EHAAQFFSTAPSTNLLATWNSVWISIASVIGAALIGIPLALFMATYDFPGRRVTSALV
                     MMPIVLPPLIGVLAFVLLFSESGLIPRGLHLFLGLNPAQWSLGGAWGVVAVHAYSFYV
                     FFYTFVGAALEGRDPSLDEAARSLGAGRWLRMRRVTLPLLSPAIIGASLLVFMVSMAS
                     FSAPLIFGGDFRVLSVHIYQTKLSGNLGLAAIQTVVLSMISLGFLILIRAWGRRRRTV
                     MSTKGAVRSRRALPAGAVRILLPPVAIGFALLALLPHLTLALLSFTKQGTWTFRFSSW
                     LPTEYTFANYRYLLDVMTPLVHSLQLAAIATVGNVIFGVAVGWLVTNRSFRWGRVVDV
                     LVMLPWALPGTVVAINLISAFNTPNVFSFGGVLVGSFWLLPIAYFVRNLPLVARAATA
                     AFEQFDPVYDEAARSLGASSWMRLRRVT"
BASE COUNT          452 a          603 c          625 g          329 t
ORIGIN      
        1 gcgtcacccg gcgcaatcgc atccacgatg acgcgccgag gctccgcgcg gcttcgtcgt
       61 aaaccggatc aaactgctcg aacgccgccg tcgccgcacg cgccacgagc ggaagattcc
      121 ggacgaagta cgcgatcggc agcagccaga acgatcccac gagcacaccg ccgaagctga
      181 agacattcgg tgtgttgaac gcgctgatga ggttgatcgc gacgaccgtg ccgggaagcg
      241 cccatggaag catgacgagt acgtcgacga cgcgacccca gcggaatgat cgattcgtga
      301 cgagccaacc gacggccacg ccgaagatga cgttgcccac ggtcgcaatc gccgccagtt
      361 ggagactgtg gacgaggggc gtcatcacat cgagcaggta ccggtaattc gcgaaggtgt
      421 actcggtcgg cagccatgac gagaagcgaa acgtccatgt gccctgcttc gtgaatgaaa
      481 gcaacgccaa tgtcaggtgc ggtaacagtg caagcagcgc gaatccgatt gcgacgggcg
      541 ggagaaggat gcgtaccgca cctgccggga gcgctcgtcg gctgcgtacg gcacctttcg
      601 tcgacatgac ggttcggcga cggcgacccc acgcgcgaat gagtatgagg aatccgagcg
      661 agatcatgga gagcacgacg gtctgtatcg ctgcgagacc gagattgccg ctgagcttcg
      721 tttggtagat gtgcacgctc agcacgcgga agtcgccgcc gaagataagc ggcgcactga
      781 aagacgccat ggagaccata aacaccaaca acgacgcacc gataatcgcc ggactgagaa
      841 gcggcaacgt cacgcggcgc attcggagcc aacgccccgc accgaggctt cgcgcggctt
      901 cgtcgagtga gggatcgcgt ccttccagtg ccgcgccgac gaacgtgtag aagaagacgt
      961 agaaactgta cgcgtgaact gctacaacgc cccacgcgcc gccaagcgac cactgcgccg
     1021 gattcagccc gagaaacaga tgcaggccgc gcggaatgag gccgctttcg gaaaagagca
     1081 gcacaaacgc gagtacgcca atgagtggcg gcaggacaat cggcatcatg acgagcgcgg
     1141 atgtgacgcg acgaccgggg aagtcgtacg tcgccatgaa cagtgcgagc ggaataccga
     1201 tcagtgccgc gccgatgacc gacgcgatac tgatccacac gctattccac gtcgcgagga
     1261 ggttcgtcga gggggcggtc gagaagaatt gtgcggcgtg ttcgacggtg aacgatccgt
     1321 ccacgcttcg gaacgcctcg aagacgacgg cgatgcttgg atagacaatg tatccagcga
     1381 ggacaaatcc gacggctgaa atgatgagta tgcgaagatg acgggtagag tgcataggtg
     1441 acctcgcgtc tcctcgctcc gctcggagac gttaaggcga agactgatgt cttcgcctta
     1501 ttattttcag acgcgacgtt ggtttcgcgg tgtcttgggt gcccggcgga taaatccgcg
     1561 ggggttctcc gcgccggatc ctacaatgcg tcgttcgttt cgcgaagcgt ttactcggta
     1621 cgtcgatttc gcggtgcgac taaagtcgcg cgtctccccg ctgcgctcgg agacgtaggg
     1681 cgaaggctcc gccttcgcct aatcgagccc gcaagggctc gcgctacacg tacaaagtct
     1741 gccttcgcag actcgaccga acacgttaac cgtaccgcca gtccgcgtag gcggacttcg
     1801 caatccgagc ccgcgaattc attcgccggg ctcctatccg ccttacgcac ccttacgtac
     1861 gggcgaggcg tgcctcgcac cctaccgaat cacgtcccat tcgaagccaa agcgggcgaa
     1921 ggcaatgaat gcgcagcatt cgcccttcac ctatacgagc gggcgaagcg acgttgtcgc
     1981 ttcgccttaa cgaactcgaa cgcacgtga
//
LOCUS       DSCC01000319            1751 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046097, whole genome shotgun sequence.
ACCESSION   DSCC01000319 DSCC01000000
VERSION     DSCC01000319.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046097"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..324
                     /locus_tag="ENN56_03940"
     CDS             <1..324
                     /locus_tag="ENN56_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HDS74673.1"
                     /translation="LMAWAFGGRTFKLKFGHRGVNQPVMNHDTGKVEITAQNHGFAID
                     PASLPDYLRVTHTNLNDRTVEGLQHTRLPIFGVQYHPEASSGPHDARYLFRRFDKFME
                     TYLAS"
     gene            532..978
                     /locus_tag="ENN56_03945"
     CDS             532..978
                     /locus_tag="ENN56_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDS74674.1"
                     /translation="MATYGKGTYTVSGKDIKPSWYVVDAEERVLGRLASQIARVLKGK
                     HRPDYSPHLDLGDRVIVVNAAKLRLTGKKLEQKTYFRHSGYPGGAKFTSLSSLMENDP
                     AKVIEFAVQGMLPKGKLGRELLRKLRVYAGPENPHIAQNPEPLPEM"
     gene            1009..1401
                     /locus_tag="ENN56_03950"
     CDS             1009..1401
                     /locus_tag="ENN56_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDS74675.1"
                     /translation="MDNPIYEATGRRKESTARVRITPGTGQIIINKRNAAEYFARPTL
                     VMQVEQPLKITETLGSYDVRVTAKGGGLSGQAGAIRHGIARALERMNPDLRPPLKVAG
                     LLTRDPRMVERKKPGQPGARKKFQFSKR"
     gene            1677..>1751
                     /locus_tag="ENN56_03955"
     CDS             1677..>1751
                     /locus_tag="ENN56_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002956179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDS74676.1"
                     /translation="MKDLLEAGVHFGHQTRRWNPKMKRF"
BASE COUNT          376 a          475 c          514 g          386 t
ORIGIN      
        1 cttatggcgt gggcgttcgg cggtcggacg ttcaaactca agttcggtca ccgcggtgta
       61 aatcagccgg tgatgaatca tgataccggg aaggttgaga taaccgcgca gaatcacgga
      121 ttcgcgatcg atcccgcatc gctaccggat tacctccgtg tgacgcatac gaacctcaac
      181 gaccgaacgg tcgaagggct ccagcacaca cgtctaccga tattcggtgt gcagtatcat
      241 ccggaggcgt catcgggtcc gcatgatgca cggtatctct ttcggcggtt cgacaagttc
      301 atggaaacgt acttggcgtc ttgacgtgag gttggctttt cggttacatt agtaaatcta
      361 ttcccctaat cgtcgcagta tcgtcgattg agtaccgatt gccgctggtc tgatcggtcc
      421 cgatcaccca tctcggtgcc ccaggacggg cgggacgggt tccgtttccg gcgctcgagc
      481 ggatgatcgt gattgacggg cgacgttttt ctctgtctgt cggagtcgaa catggcaaca
      541 tatggtaaag gcacgtatac cgtcagcggc aaggatatca agccgtcgtg gtacgtcgtg
      601 gacgccgagg aacgcgttct cggacggctt gcgagtcaga tagcgcgggt actcaagggc
      661 aaacatcggc cggattactc accgcacctc gatctgggtg atcgggttat cgtcgtaaac
      721 gcggccaagc ttcgtctgac gggcaaaaag ctggaacaga agacgtattt ccgtcactcg
      781 ggatatcccg gtggtgcgaa gttcacgtcg ctttccagtc tcatggaaaa tgatcctgcg
      841 aaggtcatcg agtttgccgt acagggcatg cttccgaaag gtaagcttgg tcgtgagcta
      901 cttcgtaagc ttcgtgtcta tgctggaccc gagaatcctc atatcgcaca gaatcccgag
      961 ccgctgcccg agatgtaatg tatctcgcag cacaggagcg taacgtacat ggataacccg
     1021 atctatgagg cgaccggtcg tcgaaaggaa tcgacggcgc gggtacgcat caccccgggg
     1081 accggacaga tcatcatcaa caagcgtaac gccgccgagt attttgcgcg gccgacactc
     1141 gtcatgcagg tcgagcaacc gttgaagatt acggagacac tcggttcgta tgatgtccgc
     1201 gtaacggcaa aaggtggcgg tttgagtggt caggccggag cgattcggca tggtattgcg
     1261 cgtgcgcttg agcggatgaa tcccgatctt cgcccccccc tgaaggtcgc cggtcttctc
     1321 actcgcgacc cccgtatggt cgagcggaaa aagcccggtc agcctggtgc gcggaagaag
     1381 ttccagttct cgaagcgtta atttcggtgc gcgaaactcg ctctacgcga acttcgcccc
     1441 gttatagaaa tttcggcgaa gcggactcct ccgcttcgcc gttgcgtatt ctcacggcga
     1501 atcactctgt gtcgattcgt cgatcacaca cacgggtacg ttccgacgtc gtcccgcacg
     1561 attccaacgc gtccacagtg agagttcgga cggacatcgg tgggcttgat cggatgagat
     1621 gacgacccgt ggaggctaac gacccatcag gaggtgtcat tgtgccacgc gttgagatga
     1681 aagacctgct cgaagcaggt gtacacttcg gtcaccagac tcgtcgttgg aatccgaaga
     1741 tgaagcgctt c
//
LOCUS       DSCC01000320            1576 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053190, whole genome shotgun sequence.
ACCESSION   DSCC01000320 DSCC01000000
VERSION     DSCC01000320.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053190"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..565)
                     /locus_tag="ENN56_03960"
     CDS             complement(<1..565)
                     /locus_tag="ENN56_03960"
                     /inference="COORDINATES: protein motif:HMM:PF01019.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDS74677.1"
                     /translation="MSNRGFGWSISRPYPVHRGAVMATNGMVASAHPLATATGVDVLR
                     RGGNFIDAALATAAVLNVVEPYNSNLGGDVFLIAYDAKRNETVALNGSGVAPASATID
                     RFRSGIPTDGLAAASVPGQVHGWLTAHKRWGTWELSELFAPAIRYAEDGFPANMQLVR
                     AIAGVQHRIAQFPSSQRVFGTVPNPGDI"
     gene            complement(583..>1576)
                     /locus_tag="ENN56_03965"
     CDS             complement(583..>1576)
                     /locus_tag="ENN56_03965"
                     /inference="COORDINATES: protein motif:HMM:PF05343.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDS74678.1"
                     /translation="TDRYGNIIVRRPARGHSAPRIGFMAHMDHPGCEVVEHRAGRVIA
                     AWHGGVHQEYFVGARIRFYAADGEYTGVVEETTIDPDTHRVGTITVACDRTPPVGALG
                     MWDVEPVEFANGVIRARAIDDLAGCGVMLCVLHELGDQPLDVDVWAVFTRAEEVGFLG
                     AIGLARSGRLPTSLPVVSIEMSKSIPGGSQGMGPVVRLGDKSSVFDNGFLLYLRDIAA
                     ELKDERENFKYQQILMDGGSCEATPLSAFGFRTAGLAIPLQNYHNMKPTGPSGPWSIE
                     PEEINFSDVLAGIDLCVAMCERFPGLQAVTERHRRKLLDTTSDRIKRLEDDIKK"
BASE COUNT          354 a          484 c          406 g          332 t
ORIGIN      
        1 atatatcgcc ggggttgggt accgtgccga agacacgttg cgacgatggg aactgagcga
       61 tacgatgttg aacgccggca atcgctcgta cgagctgcat attcgccggg aaaccatctt
      121 ccgcataccg aatcgccgga gcgaataact cggaaagctc ccacgtgccc cagcgtttgt
      181 gcgccgtcaa ccagccatgt acctggccgg gaacactcgc tgcggcgaga ccgtccgtcg
      241 gaatgccgct ccgaaatcga tcgatcgtcg cggacgcagg tgcaacacca ctcccgttga
      301 gcgctaccgt ctcgtttcgc ttcgcatcgt acgcaatcag gaatacgtcg ccaccgagat
      361 tcgaattata cggctcgacg acattcaaaa cggccgcagt ggcgagtgcc gcatctatga
      421 agtttccgcc gcgacgcaga acatccaccc ctgttgcggt agcgagagga tgtgcggacg
      481 caaccattcc gttcgtcgcc atgacggcgc cgcgatggac ggggtacggt cgcgaaatgc
      541 tccaaccgaa gcctctatta ctcattgatt tgtcccagca tttcacttct ttatgtcatc
      601 ttcgaggcgt ttgatgcggt ccgatgttgt atcaagcagt tttcgccgat gtcgttcggt
      661 aacggcctga aggccgggga atcgttcaca cattgcgaca cagagatcga ttccggcaag
      721 cacgtctgag aaattgattt cttcgggttc gatactccac ggccctgaag gtcccgttgg
      781 tttcatgtta tggtagtttt gaagcgggat agccagaccc gcggtccgaa aaccgaaggc
      841 actcagcggc gttgcctcac agctcccacc gtccataagg atttgttggt acttgaagtt
      901 ctcgcgttcg tctttcagtt ctgcagcaat atcgcgaagg tagagaagaa acccgttatc
      961 aaacacgcta cttttatctc cgagtcgaac aacgggaccc atcccttggc tgccgccggg
     1021 aatcgattta ctcatctcga tactgaccac cggaagggaa gtcggcagac gtccgctccg
     1081 ggcaagtccg atcgctccga ggaacccaac ctcttctgct cgcgtgaaaa ccgcccatac
     1141 atcgacatcc agcggttgat cgccaagttc gtgcaatacg cacaacatca caccgcaacc
     1201 ggcaagatca tcgattgctc gagcgcgtat tacgccgttg gcgaactcca cgggttcgac
     1261 gtcccacatc ccgagcgcgc cgacaggcgg cgtccgatca cacgccacgg taatcgttcc
     1321 gacgcgatgc gtatccggat caattgtcgt ctcctcaacc acacccgtat attcgccgtc
     1381 cgctgcataa aatcgaatcc gtgcaccaac aaaatactcc tgatgaactc caccatgcca
     1441 cgccgcgata acgcgtcctg cacgatgctc aaccacttcg caaccgggat gatccatatg
     1501 cgccatgaac ccgattcgtg gcgcagagtg accgcgtgcg ggacgtcgca cgatgatatt
     1561 cccataacga tccgtg
//
LOCUS       DSCC01000321            2338 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031107, whole genome shotgun sequence.
ACCESSION   DSCC01000321 DSCC01000000
VERSION     DSCC01000321.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031107"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..193)
                     /locus_tag="ENN56_03970"
     CDS             complement(<1..193)
                     /locus_tag="ENN56_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74679.1"
                     /translation="MLDRLMNIDRRIIFLFIFFGVAAAMIWPIQIPIKPTRDVRAIYN
                     DIEAIADLEDPVILLSFDYG"
     gene            complement(193..846)
                     /locus_tag="ENN56_03975"
     CDS             complement(193..846)
                     /locus_tag="ENN56_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74680.1"
                     /translation="MLFMKRTLPLIIAFTVGMIGISLYYIPHESAQKLQETLAKWDRL
                     IWAFAFFLGLYSLLNLHAQRIKRRQSGWAYSVFVYIGFAAMVLSATWNGGKGPLVGRG
                     TGDLYDWFYTYIFVPSESSMFALLAFFIASAAYRTFRARTTEAAILLVAAVIVMLGRI
                     PIGALIWEQLPDLQQWLMTIPNMAAKRGIVLGVYLGAIATALRIIFGIERSYLGGGE"
     gene            complement(902..2194)
                     /locus_tag="ENN56_03980"
     CDS             complement(902..2194)
                     /locus_tag="ENN56_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74681.1"
                     /translation="MNNARARYGLLVIAFSLIIGGAASRSEALDAEAFLPARGTVELV
                     DLPNDNGTGLVVTWPTADVPSDVSVMYGFEIAPSSDGPFHFAGHMGASGPRVTDDPSI
                     FGWFFDGNNGTDKIARQYLVISGYTESNSDDVSASTRHPLTAGQQYWVRMSVAVNGSE
                     TYYAETITATPRQNWLKWNKLNNLVFGLVFGSVILVFIRLARRNPDMYIRKIAGLDAV
                     EEALGRATEMGKPVYFVHGIASMGEISTIAAVNILRPVAKRVAEYDTTLKVMNNDPIV
                     MAVSQEVVEEGCIDAGRPDAYNEDNVMFVADRQFSYAAAVDGMMVRERPATVMLMGYF
                     YAESLLFTETGGEIGAIQIAGTDAYTQLPFFITTCDYTLMGEELYAASAYLAREPKLL
                     GSLRGQDVGKAILLLLLGAGTVLELLGINWIAALVQPL"
BASE COUNT          556 a          675 c          629 g          478 t
ORIGIN      
        1 gtccgtagtc gaatgagagg agaatgaccg gatcttcgag atctgcgatg gcctcgatat
       61 cgttgtaaat agctctgaca tcacgcgttg gtttgatggg tatttgaatc ggccagatca
      121 tcgctgcggc gacgccgaag aagatgaaca ggaagatgat gcgccggtcg atattcatga
      181 gtcggtccag catcattctc ctccaccgag ataactgcgc tcaataccga agatgatccg
      241 cagcgccgtg gcaatcgcac cgaggtaaac acccaacaca atcccgcgtt tcgccgccat
      301 gttcggtatc gtcatcagcc actgctgtaa atctgggagc tgctcccaga tcagtgctcc
      361 aataggaatt cgtcccaaca tgacaatcac tgccgcgacg agaaggatcg cagcttccgt
      421 tgtgcgagct cggaaggtgc ggtacgcggc cgacgcaatg aaaaaggcga gtagcgcgaa
      481 catggacgac tcggatggca cgaaaatgta cgtgtaaaac cagtcgtaca aatcgcctgt
      541 gcctctgccg acgagcggtc ccttaccgcc gttccacgtg gcactcaata ccattgcggc
      601 gaatccgatg tagacgaata cactgtacgc ccagccgctc tgccgccgtt tgattcgctg
      661 cgcgtggagg ttgagaagac tgtacagacc gaggaaaaac gcgaatgccc atataagacg
      721 gtcccatttc gcgagcgttt cctggagttt ctgcgcgctt tcatgtggta tgtagtagag
      781 cgaaatacca atcataccga ctgtgaaggc aatgataagt ggaagcgtac gcttcataaa
      841 gagcatcgac gcgttcctcc gaaagcggat atatcgtgtc cgtcaaccat gatgaaatga
      901 ctcacaaggg ttgtacgagt gctgcaatcc aattgatccc caatagctca agcacggtac
      961 cggcgccgag tagcaataac aagatcgctt tgccgacatc ctgaccgcgt agacttccga
     1021 ggagctttgg ctcccgagcg agatatgcac tcgcggcgta gagctcctcg cccatgagtg
     1081 tatagtcgca ggtggtgatg aagaacggga gctgcgtata cgcgtcggtt ccggcgatct
     1141 gtatcgcacc gatctcacct ccggtttccg tgaaaagaag tgactcggcg tagaaatacc
     1201 ccataagcat cactgtcgcg ggtcgctcgc gcaccatcat tccatcgact gccgcggcat
     1261 agctgaactg acggtctgcg acaaacatca cattgtcttc gttgtacgcg tccggccgac
     1321 cggcgtcgat acaaccttct tccacgactt cctgactcac ggccatcacg atcgggtcgt
     1381 tgttcatcac tttgagcgta gtgtcgtact cagcgacacg tttcgcgacc ggccgtagga
     1441 tattcaccgc cgcgatggtc gatatctcgc ccattgacgc gatgccgtgt acgaaataca
     1501 cgggcttccc catctccgtt gcgcgaccga gtgcctcttc caccgcgtcg agaccggcga
     1561 tcttccggat gtacatatcg gggttccggc gtgcgagtcg gataaagacg agaatgaccg
     1621 aaccgaaaac gaggccgaaa acgaggttgt tcagtttatt ccacttaagc cagttctgcc
     1681 gcggagtcgc ggttatcgtt tccgcgtaat acgtctcgga gccgttgacc gcgacactca
     1741 ttcgcaccca gtattgctgc ccggcggtaa gtgggtgccg tgttgaagcg gacacgtcgt
     1801 ccgaattgga ctccgtatac ccggatataa cgaggtactg ccgagcgatc ttgtctgtac
     1861 cgttgttgcc gtcgaaaaac cagccgaata tcgacggatc atcggtaact cgcggtccgc
     1921 tggcgcccat gtgccctgca aagtggaacg gtccatcgga ggatggagcg atttcgaagc
     1981 catacatcac cgacacgtca gacggcacgt cggccgtcgg ccacgttacg acgaggccgg
     2041 tgccgttatc gttcggaaga tccaccagct cgacagtgcc ccgcgccgga agaaacgctt
     2101 ccgcatcgag agcttccgat cgcgacgccg ctccgccgat aataagcgaa aacgcaatta
     2161 cgagtaggcc gtaccgcgcg cgcgcattat tcatgcacga acctccgaac gagtgcaccg
     2221 atgatcaacg cgatcgatcc aatccaaaat ccgatgtccg cgatcgtgaa gagtccgagc
     2281 gatacatccg cccgagcgcc catgaacgca agcccaaacc acccaaaaac aacgtaaa
//
LOCUS       DSCC01000322            2442 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029378, whole genome shotgun sequence.
ACCESSION   DSCC01000322 DSCC01000000
VERSION     DSCC01000322.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029378"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            15..452
                     /locus_tag="ENN56_03985"
     CDS             15..452
                     /locus_tag="ENN56_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74682.1"
                     /translation="MTTDTQSLAVEAEGVIDLLRFVGGDIASIDTVAMSMPNLAAVTL
                     TFASGAHGMLTLVDGWTNGTEYAIDAMTDGRRYRWSNEWNSITIDGNENRASDSTEGQ
                     YVASVASFLTAVETNTQSPIVSDFRDAVETLRIVEKTETALLA"
     gene            474..893
                     /locus_tag="ENN56_03990"
     CDS             474..893
                     /locus_tag="ENN56_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDS74683.1"
                     /translation="MKSYREELWFNIPTRRAFINITPNVEKALGDSGIREGLCLVNAM
                     HITASVFINDDESGLHRDYDSWLEDLAPHEPINRYDHNRTGEDNGDAHLKRQVMGREV
                     VVAVTDGRLDFGPWEQIFYGEFDGGRRKRVLVKIIGE"
     gene            complement(957..1472)
                     /locus_tag="ENN56_03995"
     CDS             complement(957..1472)
                     /locus_tag="ENN56_03995"
                     /inference="COORDINATES: protein motif:HMM:PF00571.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDS74684.1"
                     /translation="MECPYCGYDNIKGVDLCGSCGTDLAGLDLPAEQAVGERRSLYDV
                     KLGELYHHKPLTVTPDTPVSEALARMKEQVHGATFVIDKESRLVGIFTERDLIHGFAL
                     GDRDLSTPVQEVMTKNPDALTVDASLSMAMNLMGTKGYRHIPIVDDDKRLIGFSSIRG
                     ILSYFRTHVVS"
     gene            complement(1501..>2442)
                     /locus_tag="ENN56_04000"
     CDS             complement(1501..>2442)
                     /locus_tag="ENN56_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDS74685.1"
                     /translation="AILAQTQKILPDLGLPREKFVFVSGIGCSSRFPYYMNTYGFHSI
                     HGRAPTLATGIKVVNPELSVWVITGDGDGLSIGGNHLIHAIRRNIDINIILFNNRIYG
                     LTKGQYSPTSELKKKTKSSPMGSVDQPINPLRIAIASEATFVARSIDIDTKHLAETLD
                     KAARHKGVSFVEVYQNCNVFNDGAYVNFTDRSVRDDRVLYLEDGKPMVFGKERDRGLR
                     IDLDTMRLEACKIGEDGVDEDDLVVHETGAESPMKAYLLTELNYPNPVPVGVFRDVSR
                     PTYDDLVHQQISDAKSLLGDGDLEKLFNTGETWNVTD"
BASE COUNT          584 a          661 c          625 g          572 t
ORIGIN      
        1 gaaggtacgt cggggtgaca acggatacgc agtcacttgc cgtcgaagcc gaaggtgtta
       61 tcgacctcct gcgtttcgtc ggcggagata tcgcctcgat cgataccgtc gcgatgtcga
      121 tgccgaacct cgccgccgtc acgctaacct tcgcatctgg tgcacacggc atgttgacgc
      181 tcgtagacgg atggacgaac ggtaccgagt acgcgattga cgcgatgacg gatggaagac
      241 ggtaccgctg gtcgaatgaa tggaactcaa tcacgatcga cggcaacgag aatcgtgcat
      301 ccgattcgac ggaaggtcaa tacgttgctt ccgtcgcgag tttcctgacc gctgtagaaa
      361 cgaacacgca gtcaccgatt gtatcggact tccgggacgc cgtcgaaaca ttgcggatcg
      421 ttgagaaaac tgagacagca ttgttggcgt aatcgacgaa aaaggagaga cagatgaaaa
      481 gctaccgcga ggagctctgg ttcaacatcc cgacacggcg tgcgttcatc aatatcactc
      541 cgaacgtcga aaaagccctc ggcgacagcg gcattcgcga gggactctgt ctcgtgaatg
      601 cgatgcatat tacggccagt gtcttcatca acgacgatga atctggcctt caccgcgatt
      661 atgacagttg gctcgaagac ctcgcaccgc acgaaccaat caaccggtat gatcacaacc
      721 gcaccggcga agacaatggc gatgcacacc tcaaacggca ggtaatggga cgcgaagtgg
      781 ttgtcgccgt gacagacggt cgcctcgatt tcggaccgtg ggaacagata ttctacgggg
      841 agtttgacgg aggacggcgc aagcgcgtac tcgtgaagat tattggcgaa taggaaggtg
      901 agaagtagct gatttcgcag ccttttacgt gaccgacttt accattagcg tcttgctcag
      961 ctcacgacgt gtgttcggaa atagctcagt attccgcgaa tgctcgaaaa accgataagc
     1021 cgcttgtcat cgtcgacaat cggtatatga cgatatcctt tcgtccccat gagattcatc
     1081 gccatactga gagaagcgtc aaccgttagt gcgtcggggt ttttcgtcat cacctcttgc
     1141 accggcgttg agaggtctcg atcgccgagc gcgaacccat gaatgaggtc acgctccgta
     1201 aatataccga ccaaacgcga ctctttatcg atcacaaacg tcgcaccgtg aacctgctcc
     1261 ttcatacgcg ccaacgcttc cgaaactggt gtgtctggag tgaccgtaag cggtttgtga
     1321 tggtagagtt caccgagttt cacatcgtag agcgagcgtc gttctcccac ggcttgctcc
     1381 gcgggtaaat cgagcccggc aaggtcggtc ccgcaactcc cacataaatc gacacctttt
     1441 atattatcat aaccgcaata cgggcattcc atcgtatcac ttccttcttg agagactcca
     1501 ctaatcggtt acgttccaag tctctccggt gttgaacagc ttttcgagat caccatctcc
     1561 gaggaggctt tttgcgtcag aaatctgctg atgcacgagg tcatcatacg tcggacgcga
     1621 aacatcgcga aatacgccaa ccggaacggg attcggatag ttcaattcgg taagcagata
     1681 cgctttcatc ggactctctg caccggtttc gtgtaccacc aaatcatctt catcgacacc
     1741 gtcctcgcca atcttacacg cttcgagccg catcgtgtcg agatcaattc ggagtcctcg
     1801 atcgcgttcc tttccgaata ccattggctt cccgtcttcg aggtacagca ctctatcgtc
     1861 acgtaccgag cggtctgtaa agtttacgta cgcgccgtcg ttgaaaacgt tacagttctg
     1921 gtacacttcg acaaacgaaa cacctttatg ccgcgcggct ttgtcgagtg tctccgcaag
     1981 atgctttgtg tctatgtcaa tcgatcgcgc tacgaaggtt gcttcagatg caatcgcgat
     2041 ccgcaaagga ttgatcggct ggtcgacgga ccccatggga ctcgacttcg tcttcttctt
     2101 taactccgat gtcggcgaat actgaccttt cgtcaaacca tagattcggt tgttgaagag
     2161 tatgatgttg atatcgatat ttcgccgtat cgcatgaatc agatggtttc caccgatcga
     2221 taatccatca ccgtcaccgg taatcaccca aacggaaagc tctggattca ccaccttgat
     2281 accggtagca agcgttggtg ctcggccgtg gatactgtga aacccgtacg tattcatgta
     2341 gtacggaaat cgactcgaac acccgatgcc ggatacgaat acgaacttct cacgcggtag
     2401 accgaggtcc ggcaggatct tctgcgtttg tgcgagtatt gc
//
LOCUS       DSCC01000323            1565 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053731, whole genome shotgun sequence.
ACCESSION   DSCC01000323 DSCC01000000
VERSION     DSCC01000323.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053731"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(83..>1565)
                     /locus_tag="ENN56_04005"
     CDS             complement(83..>1565)
                     /locus_tag="ENN56_04005"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDS74686.1"
                     /translation="TPEIFVHFNMPDHYTPEQTDSIVAKYEEFMNTRREAYGIRFVEG
                     AYWHGGGRIRAHLQPDDRSWYTIAWHNLAKAAGRPIYNRLEGEAAFADFREHAPRFAG
                     VEMSVDRSRRGSRQSAVTLFGDDTRRLITLAEEVERRLRLLPELTDVNSDMEEGEQEL
                     QISVNRERAQQYGLDGRVIASTLSYALRGMPLRAYRTDDREVDLRVRLREEDRQTLDQ
                     VLNMKVLTGAGSQVSVGSLVDVEVGRGLERIRRENGRTRVRVSAVSSEKDLKTLAELI
                     QTTLADFTMPPGYQWSLSGEFEQLNEQENNFTSAMIMAGLFVFLLMGILFESFILPFS
                     VIMAIPFSFLGVYWMLWITDTPFDMMAKIGTIVLIGVVVNNAIVLIDLINRLRAEGNE
                     RTDAILEAGSNRFRPILMTSITTSFCLTPMALSGGAMAGIEYASMGRAMIGGLLTATM
                     LTLFVVPLFYSLFDDLRVSAARLVSLLRHTGARKHAEATVHSD"
BASE COUNT          356 a          438 c          421 g          350 t
ORIGIN      
        1 aggtacacct aaatgtagct gtgcacccgt ttctgtccaa gatacacctc tcgctttgta
       61 acggtgcgag aggttgttac acctaatcgg aatggacggt cgcctctgca tgcttacgcg
      121 ctccggtatg acggagcaac gatacgagac gcgcagcgga aacacgtaga tcgtcaaaga
      181 ggctgtagaa cagtggcacg acgaatagtg tcagcatggt cgcagttaac aatccgccga
      241 tcatcgcgcg ccccatgctg gcatattcga tgccggccat cgcgccgcca ctaagtgcca
      301 tcggtgtaag acagaagctc gtcgttatac tcgtcatgag aatcgggcgg aaacggttac
      361 ttccagcttc gagtattgcg tcggtgcgct cattgccctc ggctcggagt cgattgatga
      421 ggtcaatcag tacgatcgcg ttgttgacga cgactccgat gagcacaatc gtgccgatct
      481 tcgccatcat gtcgaatggc gtgtcggtta tccagagcat ccagtagaca ccgaggaaac
      541 tgaacggaat cgccataatg acggagaagg ggagaataaa agattcgaag agtattccca
      601 tgagaagaaa cacgaacaat cccgccatga tcatcgcgga tgtgaagttg ttctcctgct
      661 cattaagttg ctcgaactca ccggagagag accattggta cccgggcggc atagtgaagt
      721 ccgccaacgt tgtctgaatc aattcagcaa gtgtttttaa gtctttttcg ctactcactg
      781 ccgatacgcg cacgcgtgtt ctaccattct cgcgtcgaat tcgttcgaga cctcggccga
      841 cttccacatc cacgagcgat ccgacggaga cctgtgaccc ggcaccggtg aggactttca
      901 tattcaacac ttgatcaagc gtttggcggt cttcctcacg aaggcgcacg cgcagatcga
      961 cttcgcggtc atcagttcga tatgcgcgaa gcggcattcc acgtaacgca taactcagcg
     1021 tcgatgcgat cacccgtccg tcgagaccgt actgttgagc acgctctcga ttgacgctga
     1081 tctgcaattc ctgttcgcct tcctccatat cgctgttgac atcggtaagc tcaggcagaa
     1141 ggcgcagtcg ccgttcgacc tcttcggcga gcgtaatgag ccgtcgggtg tcgtcaccaa
     1201 acagtgtgac cgcggactga cgagatccac gacgtgaacg gtcgacgctc atctcgacac
     1261 cggcgaatcg cggcgcgtgc tcgcgaaaat ccgcgaacgc agcttcaccc tcgagcctat
     1321 tatagatcgg ccgtccggcc gctttcgcaa gattgtgcca cgcgatggta taccacgacc
     1381 ggtcatccgg ctgtaggtgc gcgcggattc gaccgccgcc atgccaatac gcaccttcaa
     1441 cgaagcggat accatatgcc tcgcgacgtg tgttcatgaa ctcttcgtat ttcgcgacga
     1501 tgctgtcggt ctgttcaggc gtgtagtgat cgggcatgtt gaaatgcacg aatatttcgg
     1561 gagta
//
LOCUS       DSCC01000324            2871 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023727, whole genome shotgun sequence.
ACCESSION   DSCC01000324 DSCC01000000
VERSION     DSCC01000324.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023727"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            35..919
                     /locus_tag="ENN56_04010"
     CDS             35..919
                     /locus_tag="ENN56_04010"
                     /inference="COORDINATES: protein motif:HMM:PF13088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDS74687.1"
                     /translation="MKLDVVNTLHRIDRNRQGATVQARSRDGGDTWVLETDRLPSGSP
                     EPFSGRMRFDDPNFGMKSHGSQFYLTYDRAKSWEGPYRLPLSDELGIQARTDYQVLGP
                     NECLVFLTAVKTNGKEGRPYCMYTDDGGATWSFRSWIGPEPVGYSIMPSSVRLPSGRL
                     LCAIRRYEPGEGDTQICFIELWSSDDVGKTWTFMNEPTNTGQHSGNPPALIRLRDGRL
                     ALSYAYRSEPYGIRARTSEDDGVTWSDEIHLRDDGGEADLGYTRTVQRPDGDVLTVYY
                     YNVDPMGERFIGGTIWTP"
     gene            957..2762
                     /locus_tag="ENN56_04015"
     CDS             957..2762
                     /locus_tag="ENN56_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74688.1"
                     /translation="MLEQMKRHGAAFCTVAALGMSMLAGCAGTGKMATAIPMGTIETF
                     ATPLAPDPEPERVHNLMFGTISPGYLADNADEFAAKGIDGFMMAGLMRNWDSDIWAQP
                     TQWTPDAPKGRVGGEENPLFQLCRRMNEQSRAAGIEYNSVKIAFYSYVPDWFDDAGWA
                     TLVENFRQGAIFARDAGFAGITLDIEYISEIYNLDYEAYLAPEYPRDQLREKARQRGN
                     EIMAAMLGEFPNMINWQLPAGIMDYGPLAGDLFIGMVDALAEADAPGGFHLSPERTYL
                     LTEPSAIITFANRLDTLIRSTLRDHDRKALDYWERRGTINYGLWPLGYYRGIYDENGE
                     LLGYSGKQARFGNEIVGSYADKSENYSAEEFQRQYATARTVARRFVWIYGHGPVVWQM
                     TPEEKARYHASDRDTAATVANLDEYLDVLRNPRVLGNSWIQSSALAVRNGDEPNGFLG
                     YPPVWRMSAAPSIDPTSLGTVAPATEFTRVARPDAFGYIDLKQLYPESGNSPVVWAKA
                     TFENDRPQRVFIGMGSNDDAVVYVNGKQVFHVETERGRPELFDDDIIAMNLSAGRSEV
                     VIGVADRGGSQWGFYLRIADGTGKQADGIRWTTVE"
BASE COUNT          626 a          818 c          875 g          552 t
ORIGIN      
        1 gttcgaggtc gtcgtcgggt tctcgtttgc gtacatgaag cttgatgttg tcaacacact
       61 ccatcgaatc gaccggaatc gacagggtgc gacggtgcag gcgcggagtc gcgatggcgg
      121 cgatacgtgg gtgctggaaa ccgacagact gccctccggt tcgccggagc cgttttccgg
      181 tcggatgcgg ttcgatgatc cgaattttgg gatgaaaagc cacggctctc agttctacct
      241 cacgtacgac cgcgcgaagt cgtgggaagg tccgtatcgt ctcccgctca gcgatgaact
      301 cggcatacag gcacgcaccg attatcaagt cctcggtccg aatgaatgcc ttgtgtttct
      361 cactgcggta aagacgaatg ggaaagaagg ccgtccgtac tgcatgtata ccgacgacgg
      421 cggcgcaacg tggtcgtttc gctcatggat cggtccggag ccggttggct acagtatcat
      481 gccgtcgtcg gtgcggctac cgtcggggcg gttgctctgc gcgatccgtc gatacgaacc
      541 gggtgagggc gatacgcaga tttgctttat cgagctctgg tcatcggacg atgtcggaaa
      601 gacatggacg ttcatgaacg agccgacaaa caccggtcaa cacagcggta atccgcccgc
      661 actgatcagg ttgcgagacg ggcggctcgc actgagttac gcgtaccggt ctgaaccgta
      721 cggtattcgc gctcgcacga gcgaggatga cggtgtaacg tggtccgacg aaatccacct
      781 tcgcgacgac ggtggcgaag cggacctcgg ttacacacgc acggtgcaac gaccggatgg
      841 tgatgtactc accgtgtact actacaacgt cgatccgatg ggcgaacgct tcatcggcgg
      901 cacaatctgg accccttaac cacactccaa gaactccacg aaaggatcac cgggttatgc
      961 tcgaacaaat gaagcgccac ggtgctgcgt tttgcaccgt ggcggcactc ggcatgtcta
     1021 tgctcgccgg atgcgcggga acggggaaaa tggcaaccgc aatcccgatg ggcactatcg
     1081 aaacgttcgc aactcctctt gcaccggatc cggaaccgga acgcgtgcac aacctcatgt
     1141 tcggaacgat ttcacccgga tacctcgctg ataacgccga cgaattcgcc gcgaagggca
     1201 tcgacggatt catgatggcg ggcctcatgc ggaactggga tagcgatatc tgggcgcaac
     1261 cgactcagtg gacgccggac gcgccgaagg gccgcgtcgg cggcgaggag aatccactct
     1321 tccagctttg ccgccgaatg aacgaacagt ctcgtgctgc ggggattgaa tacaacagcg
     1381 tcaagatcgc gttctattcg tacgtgccgg actggttcga tgacgccggc tgggcgacgc
     1441 ttgtcgagaa cttccgtcag ggagcgatct tcgcgcgcga cgcaggattc gccggtatca
     1501 cgttggacat cgaatacatt tccgagatct acaatctcga ctacgaggcg tatctcgcac
     1561 cggaatatcc gcgcgatcag ctgcgcgaga aagcgcggca gcgtggaaac gagatcatgg
     1621 cggcgatgct cggtgagttc cccaacatga tcaactggca gcttcccgcc ggcatcatgg
     1681 actacggacc gcttgcaggc gacctgttta tcggcatggt cgacgcgctc gccgaagccg
     1741 atgcaccggg cggatttcac ctttcgcccg aacggacgta cctcctcacc gaaccgagtg
     1801 cgattatcac gttcgccaac cggctcgata ccctcatacg aagcactctc cgcgaccatg
     1861 atcggaaggc gctcgattac tgggagcgcc ggggtacgat caactacggt ctgtggccac
     1921 ttgggtacta ccgtgggatc tacgacgaaa acggcgagtt actcgggtat tcgggaaaac
     1981 aggcgcgttt cggcaacgag attgtcggat cgtatgccga taagagcgag aactacagcg
     2041 ccgaagaatt ccaacggcag tatgccaccg cacggaccgt ggcgcggcgt ttcgtctgga
     2101 tctacgggca cggaccggtg gtgtggcaga tgacgccgga ggaaaaagcg cggtaccacg
     2161 cgagcgatcg ggatactgcg gcgaccgtgg caaacctcga cgagtacctc gacgtactgc
     2221 gcaatccgcg ggtcttgggg aatagttgga ttcaatcgag tgcgctcgca gtgcgaaacg
     2281 gtgatgaacc gaatggattc cttggatatc cccctgtttg gcggatgagc gccgctccct
     2341 ccatcgatcc gacgtcgctg ggaacggtcg caccggcaac cgaatttacg cgcgttgctc
     2401 gccccgatgc attcggctat atcgatctca agcagctgta tccggagtcc ggtaactcac
     2461 cggtggtgtg ggcgaaggcg acgtttgaga acgaccgtcc gcaacgggtg ttcatcggaa
     2521 tgggaagcaa cgatgacgcg gtcgtttacg taaacggaaa acaggttttc cacgtcgaaa
     2581 cggaacgcgg gcgtccggag ctcttcgacg acgatatcat cgccatgaac ctgtccgccg
     2641 gacggagcga agtcgtcatc ggtgtcgccg atcgcggcgg ctcacagtgg ggcttctacc
     2701 tccgcatcgc cgatggcacc ggcaagcaag ccgatggtat tcgttggacg accgtcgagt
     2761 agccgtcaat gaatcgggta caaaaaaaca gggacgccgg agcaaacttc gccggcgtcc
     2821 ctctgtgtac tgagctaaca atacaatgtg cctatccgaa acgttaacga a
//
LOCUS       DSCC01000325            2474 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028881, whole genome shotgun sequence.
ACCESSION   DSCC01000325 DSCC01000000
VERSION     DSCC01000325.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028881"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..713)
                     /locus_tag="ENN56_04020"
     CDS             complement(<1..713)
                     /locus_tag="ENN56_04020"
                     /inference="COORDINATES: protein motif:HMM:PF00672.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDS74689.1"
                     /translation="MSGQRSWWSIIYDLPLRYKIIVPVIVLELFVFAAAMRVTTTSVR
                     AVANRSMHTEARSRLRLLGSAYLQMTLTGEQEYFRELVNVVSRAPDVVSVELRTVEDA
                     LPVDTMVERPDGTRLYTAWQRVQVFGDATDIGISYTDQGVLLAVSDARTRYGWILGIG
                     MILTTVVLWAMIFAITDPVSRVAEAVGRLAAGEEPPKFDISRRDEVGKLAHAFDSMAE
                     QLHNARIQLEAMLAERTRRL"
     gene            complement(831..1298)
                     /locus_tag="ENN56_04025"
     CDS             complement(831..1298)
                     /locus_tag="ENN56_04025"
                     /inference="COORDINATES: protein motif:HMM:PF08741.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74690.1"
                     /translation="MVSRELPMAGGQSVDPGTGVTLLAFVLDGVSVRHDSGALHGRSR
                     EETDIVFSENPDAARNGRPVTIVWVGVRAGIDGRDGYFSAVASTQRIDAEAGTGYRPS
                     THFTDMIKAIQGENAIGLLSSDAKTALARAMVEHSAELWANRSALLKDLHAEQ"
     gene            1447..2079
                     /locus_tag="ENN56_04030"
     CDS             1447..2079
                     /locus_tag="ENN56_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74691.1"
                     /translation="MARFHRRVFVVVVLALVMFPGIVAAQLEINGVPIRGGEYTVELN
                     PSVASGQLFQTYVSRVGKDGFDQVMFRDEEGRLWLHYGRHLLLYASSANRVNQASFKG
                     DPVTIIHIDDENEEIDIRQPLGRRIAAILIFIVFVGLGLGILSGLVARRNFIILGKNP
                     LWDHALYGLVVGVIVALLVYGLIDRRFDPDKLSATYTHDHLKPFMLNPPN"
     gene            complement(2076..2474)
                     /locus_tag="ENN56_04035"
     CDS             complement(2076..2474)
                     /locus_tag="ENN56_04035"
                     /inference="COORDINATES: protein motif:HMM:PF09515.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74692.1"
                     /translation="MRERADGSSDTLTAITSHGEKARDWMTQAVNRLRTEYTRWFGGI
                     VVLLVMTGGIAILSRYLSIGNVALGVLIGGALVFAAHFFAGVIFFAQYAPAGSSVWVY
                     SAIYNASYVTPQIVLSLIILPPLLRRAQRH"
BASE COUNT          523 a          762 c          657 g          532 t
ORIGIN      
        1 aggcgccgtg ttcgctcggc gagcatcgcc tcgagctgaa tccgcgcgtt gtgaagctgc
       61 tccgccatgc tgtcgaaagc gtgcgccagc ttacccactt cgtcacgtcg cgatatgtca
      121 aacttcggag gctcctcgcc tgccgccagt cgaccgacag cttcggcgac tcgagatacc
      181 ggatcggtta tcgcgaagat catcgcccac aacacaacgg tcgtcaaaat cataccgatt
      241 cccaaaatcc aaccgtaccg ggtacgcgca tcgctgaccg ccaataaaac tccttggtca
      301 gtgtagctga taccgatatc ggtcgcgtca ccaaacacct gaacgcgttg ccatgccgta
      361 tacagacgcg taccgtccgg gcgctcgacc atggtgtcca cgggaagcgc gtcttcgacc
      421 gttcgcagtt ccaccgaaac gacgtccggt gctcgactga cgacgttgac cagctcgcga
      481 aaatactctt gctcgccggt gagtgtcatc tggagatatg ccgatccgag caggcgtagg
      541 cgcgaacgtg cctcggtgtg catgcttcga ttcgcgacgg cgcggactga cgtcgttgtc
      601 acgcgcatag ccgcagcgaa cacgaacaat tcgaggacga ttacggggac gatgatttta
      661 taccgaagcg gaagatcgta gatgatcgac caccacgagc gttgcccgct cattcggtgt
      721 tctccaaagg ggtctctccg gaatactccg gataatatcc gatcagccac tcagtgcaat
      781 tacgacaaca cgataccatg tagaaaaata tacggaacat cccccgtatt tcattgttcc
      841 gcgtgcagat ccttcagcaa tgcactgcga ttcgcccaca attcggcgct atgttcaacc
      901 atcgctcgtg cgagcgcggt tttcgcgtcc gaggacaaca atccgatcgc gttttcgccc
      961 tgaatcgctt tgatcatgtc ggtgaaatgc gtagaagggc ggtatccggt tcctgcctcc
     1021 gcgtcgattc gctgagtgct tgccacggcg ctgaaatacc cgtcacgacc gtcgatgccg
     1081 gcgcgcacgc caacccagac gatcgtcacc ggacgaccgt ttcgtgctgc gtcggggttt
     1141 tcggagaaca cgatatccgt ttcctcgcga cttcgcccgt gcagcgcgcc ggaatcgtgt
     1201 cgcaccgata ctccgtcaag aacaaacgca agcagcgtga cacccgtgcc cggatcgacc
     1261 gattgtccac ctgccatcgg taattcgcgc gacaccatta ccgaacctcc gatctacagt
     1321 gatcccgaaa tccttgacct tccgacagag tcgtgtaact tctgttgcgc gagcggcctc
     1381 gacgccggtc gtcgcgtaaa cgttttccgt cacaagcagt atagaccttc gtccttgagt
     1441 gtgccaatgg ctcgattcca tcgtcgcgtg ttcgtcgtcg ttgtgctcgc gttggttatg
     1501 ttccccggca tagtcgccgc gcaactcgaa atcaatggcg tgccgatccg cggaggcgaa
     1561 tacaccgtcg aactcaatcc gtccgtcgca tcgggacaac tgttccagac gtatgtttcg
     1621 cgcgttggga aagacgggtt cgatcaggtc atgtttcgcg acgaagaagg gcgactctgg
     1681 ctccactacg gccgtcatct cttgctctat gcgagctccg cgaatcgcgt caaccaagcg
     1741 tcgttcaaag gtgaccctgt caccatcatc cacatcgacg acgaaaacga ggaaatcgat
     1801 atccgtcagc cgctcggtcg acgtattgca gcgattctca tcttcatcgt ttttgtgggg
     1861 ctcggtcttg gaatcctttc ggggcttgtc gctcgacgga atttcatcat actcggcaag
     1921 aacccgctat gggaccacgc actctacggg ctggtcgtcg gggttatcgt cgcgctactc
     1981 gtctacggtc tgatcgatcg gcggtttgat ccggataaac tttcggcgac ctacacgcac
     2041 gatcacctga agccgttcat gctgaacccg ccaaattagt gtcgttgcgc tcgccggagt
     2101 agtggtggga gaattatgag cgataggacg atctgcggcg taacgtaact cgcattatag
     2161 atcgctgagt agacccacac gctcgaaccc gccggagcat actgcgcgaa gaaaatgacc
     2221 cctgcgaaaa agtgcgctgc gaacacgagc gcgccaccga tgagtacacc gagcgcaaca
     2281 tttccgatgc tgagataccg ggaaagtatc gctattccgc cagtcatcac aagtaacacg
     2341 acgatcccac cgaaccaccg cgtgtactcc gttcgcaacc ggttcaccgc ttgtgtcatc
     2401 cagtcgcgcg ccttttcgcc gtgcgacgtg atcgcggtca acgtgtcgga cgacccgtcg
     2461 gcgcgctctc gcat
//
LOCUS       DSCC01000326            1560 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053945, whole genome shotgun sequence.
ACCESSION   DSCC01000326 DSCC01000000
VERSION     DSCC01000326.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053945"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            172..>1560
                     /gene="mutL"
                     /locus_tag="ENN56_04040"
     CDS             172..>1560
                     /gene="mutL"
                     /locus_tag="ENN56_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDS74693.1"
                     /translation="MEPSPNRERRIRILPDRLANLIAAGEVVERPSAVVKELVENAID
                     AGATTVAIELEAGGRRRIQVVDDGCGMNADDAVLALERHATSKIADPRDLDAIASYGF
                     RGEALPSVAAVSKLQLQTCNNTDGIGTEVFIEGGQLRSVKEVGRARGTTVTVRNLFFN
                     TPARRKFLRTDQTELRHCTREVTGLALGAPEVGITLTHNGRTLFECRPNRSWQDRAIG
                     LFGREIIQRGVELDHSHETGRVYGLLGRPGDAGGGYRDQYLLVNGRSIQARTLRKAIL
                     DAYENTLESGSQPFFLIFVDIDPGSVDVNVHPQKREVRFRDERAVYTFVRGAITAAFS
                     GFDIDRKQSASTTQDDGKTAEPLGVAHFWRDARNTFVDRPSRVSDISRTSPSPSSNTN
                     VPPQRKPQSFEDRDAVIEPLRTINKKQHDDTQTSLRVPRKSDLPSEQNDQEEIALNRD
                     TKFWQFQNKYIFA"
BASE COUNT          384 a          450 c          420 g          306 t
ORIGIN      
        1 caatggccga cgagcgaagt cgtttttatc gtagacgcag cgcgtccaga cctgaagaca
       61 cagctccagc gaggtcgatt ccggtatttc gcccgtccgt ttcagatcga cgacttgtta
      121 cacctcgtcg acggactact cgatcggaga aaggtaaccg gcaggtaacg catggaaccg
      181 tcgccaaatc gcgaacgaag aatccgcatc cttccggacc gactcgcaaa tctcattgcg
      241 gcgggcgaag tagttgagcg tccctcggca gtagtgaagg agcttgtaga gaacgccatc
      301 gacgcgggtg caacgaccgt ggcgattgaa ctcgaagccg gtggtcggcg tcgcatacag
      361 gttgtcgacg atgggtgtgg aatgaacgcc gatgacgctg ttcttgccct cgagcgacac
      421 gccacttcaa agatcgccga tccgcgtgat ctcgacgcga tcgcgtcgta cggtttccgg
      481 ggcgaagcgc ttccaagtgt cgcagcggtc agtaaactcc aactccaaac ctgcaacaac
      541 actgatggaa tcggcacgga agtgttcatc gaaggtggcc aacttcgctc ggtaaaagag
      601 gtaggtcgcg cacgcggaac aaccgttact gtacgaaatc tcttttttaa tactcctgcg
      661 cgacggaaat tccttcgcac cgaccagacg gaactgcgac attgcacgcg ggaagtaacc
      721 ggactcgcgc ttggcgcgcc tgaagtcggc atcacactga cgcacaacgg tcgcacgctc
      781 tttgagtgcc gcccgaaccg ctcgtggcaa gatcgcgcaa ttggtctttt cggacgcgaa
      841 atcattcaac gcggcgtcga actcgaccac tcacacgaaa ccggacgggt gtatggactg
      901 cttggtcgac cgggcgatgc gggaggtggg tatcgtgatc agtaccttct tgtgaacggt
      961 cgatcgattc aggcgcgaac gcttcgtaaa gcaatccttg acgcatacga aaacacgctt
     1021 gaaagcggat cccagccgtt tttcctcata ttcgttgaca tcgatccggg tagcgtcgat
     1081 gtcaacgtgc atccacaaaa gcgcgaggta cgattccgcg acgaacgcgc cgtgtacacc
     1141 ttcgtcagag gggcgataac cgccgcattt tccgggttcg acatcgaccg gaaacaatct
     1201 gcatccacca cacaggacga cgggaaaact gcggaacctc tcggtgtagc acacttctgg
     1261 cgagatgcgc gaaacacatt cgtggaccgc ccttcgcggg tctccgatat ctctcgaacg
     1321 agcccttcgc cctcctcaaa caccaatgta ccacctcaac ggaaacctca atcttttgaa
     1381 gatcgagatg ccgtcatcga accgctgcgc accataaaca aaaaacagca tgacgacacg
     1441 cagacatcgc tgcgcgtacc aaggaagagt gatctgcctt ctgagcagaa tgatcaggaa
     1501 gagatcgcac tcaatcgcga tacgaaattt tggcagtttc agaacaaata catcttcgcg
//
LOCUS       DSCC01000327            4600 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1012567, whole genome shotgun sequence.
ACCESSION   DSCC01000327 DSCC01000000
VERSION     DSCC01000327.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1012567"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(78..290)
                     /locus_tag="ENN56_04045"
     CDS             complement(78..290)
                     /locus_tag="ENN56_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74694.1"
                     /translation="MGLLYEKQKRTASDKEMPDIKEIKRTTPPPRTIGTLDDAWDDEL
                     TDIDETEEVDAIDTELDTEEYDDALI"
     gene            556..1221
                     /gene="rpe"
                     /locus_tag="ENN56_04050"
     CDS             556..1221
                     /gene="rpe"
                     /locus_tag="ENN56_04050"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDS74695.1"
                     /translation="MSDSRNVLIAPSVLAADFARLRDELARVESGGADWLHLDVMDAH
                     FVPNLTFGPFIVEAIRSLSDRLLDTHLMITDPVGYAKRFVDAGSDLITFHIEAVDDPV
                     DAIHRIADTGAQVGISVKPATEVEALFPLLSELDLVLIMSVEPGFGGQKFMPNSLEKI
                     RSLRKEIDRCGYKTRIQVDGGINPETAKLVRDAGVDVIVAGTAVFRADDPARAIAALR
                     ETP"
     gene            1235..1825
                     /locus_tag="ENN56_04055"
     CDS             1235..1825
                     /locus_tag="ENN56_04055"
                     /inference="COORDINATES: protein motif:HMM:PF03167.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74696.1"
                     /translation="MCKMPTTETRIPPNGSTTQSLTIVSDRISGYEATAGNLFGGVVG
                     SVLSRILGAPGVGIPMDSIYSTAVIPWRLSRDRNPRRGEINGRRDSLAAELTTVQPLV
                     IVALGDISAYALFADRPFEQGEWRTWKALGFSAPTFCTLHPREALWNHIDEGRFLADP
                     RTKKRRMWDDWRAIADRYNNVRERGTSPDDGLEENR"
     gene            1822..2673
                     /locus_tag="ENN56_04060"
     CDS             1822..2673
                     /locus_tag="ENN56_04060"
                     /inference="COORDINATES: protein motif:HMM:PF12706.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74697.1"
                     /translation="MKVTILGSGAAEGIPAFYCRCRVCRNAAESRGRNIRTRASALFD
                     DTLKVDYGPDTYAQTLMHGVDMRAVRIIVISHAHEDHFTPTEFIWREKNFIQDGGPYA
                     VELYGNRTVGELFDGLAIRYGISREELFQRLSLSYSEAKPFLAIDAEPYRIYPIAATH
                     EPKQVALNYVIDRNGVRVLVGFDTSRYRPETWDYLTELCCDAPFDAVLMDCTMGKYGG
                     GDTHMGIEDNERVREEMYTLRIVGEATRYVLTHISHGGDFTHDELAERANSIGMLAGY
                     DGMALVL"
     gene            complement(2703..3311)
                     /locus_tag="ENN56_04065"
     CDS             complement(2703..3311)
                     /locus_tag="ENN56_04065"
                     /inference="COORDINATES: protein motif:HMM:PF02517.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDS74698.1"
                     /translation="MTVELITLIAAGVMWGVMFGLEWGNFWLNMTGAATILAITGIIV
                     MRSQLQRIFRFHAGHVAIGLVSAVVLYVVFWVGDFVSAAIFPFAVDQVGGIYAMKTQM
                     NPLTIGLLLFFLIGPAEEVFWRGFLQDRFARRWGNRVGWIAAGFVYAIVHVWSWNFML
                     VMAALICGMFWGWMYLRWKSLWPGIVSHAVWDVLVFLILPIR"
     gene            complement(3308..>4600)
                     /locus_tag="ENN56_04070"
     CDS             complement(3308..>4600)
                     /locus_tag="ENN56_04070"
                     /inference="COORDINATES: protein motif:HMM:PF01040.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDS74699.1"
                     /translation="ESIGRIERIIRSAGKITLRSTVDEHLGVSVAPGNDGIYLAIDDG
                     GEVHTAILHDPSVEFDATADDEQLSSNGRAIILGTSVEEPEARNFFIKIGANIPDTAR
                     LVRVIPHVIHLSDTSGTKTVEFPYGQRPPGILAKWWQASRPFAYTASVTPMLLGAVLA
                     WYLQVDTVSWWLLPFIIAAGVFYHAGANLVSDYFDYKRGVDRFRTMGGSRVLTDGYLA
                     PRAVLFGGLILFLAGTSLGLWMVTLRGTPLLWLGIVGLIGGFFYGGWPIEFKYRGFGE
                     TVIFTLFGPLMVIGSFYVLTGSFSRDVFFISLPVGFLVAAIVQANNLRDIADDATAGI
                     VTISNTFGQSFAAIEYYAMVISAYIGVIIMIAFGVLPFWTLIVALSILPASKVVNVIR
                     SAGGTHTSDLAIIDQMTAQVHLLFGVLLMIGIVLGKLI"
BASE COUNT         1104 a         1247 c         1210 g         1039 t
ORIGIN      
        1 aagtacttgc aggggtgatg gggctgtcac gcatatgcgt gtacagcccc agttaacgct
       61 cgattccgtt ggcgcggtta gatgagtgca tcgtcgtatt cctcggtgtc gagttccgta
      121 tcgattgcgt cgacttcctc ggtctcatcg atatcagtca gctcatcatc ccaagcgtcg
      181 tccagtgtcc cgattgtccg cggcggcggc gttgtccgct tgatttcctt gatatcgggc
      241 atttctttgt cgctcgcggt ccgtttctgc ttttcgtata acagtcccat gtctctcacc
      301 tcccttcaca atggtatgtc gtaaacgttt tctctttcga ttttcgtatg caatcctcgg
      361 gccataattt ttcgttcgct acaaatccgc gacaaataag tatcgcgatg cagtgtatgt
      421 caagaagcga acggtaatcg atcaattcgc aacacacgct ctccgagggt ggcaactaca
      481 ccattcatta cggtaatttc ccctctcgcg tgtaaggtta cttcgaaatc cataactgta
      541 cacgaaagga tacgaatgtc cgattcccga aacgtactca tcgcaccgtc ggttctcgct
      601 gccgattttg cgcgccttcg cgatgaactc gcgcgtgtcg aatccggcgg agctgattgg
      661 ctccacctcg atgtcatgga tgcacatttc gtgccgaatc tcacgtttgg accattcatc
      721 gtcgaggcga ttcggtcact cagcgaccgt ctactcgata cgcacctcat gataactgat
      781 ccggtcggat acgcaaaacg gttcgttgat gccgggagcg atcttataac gtttcatatc
      841 gaggcggtcg atgacccggt tgatgcaatt catcgaatcg cagacaccgg agcacaagtc
      901 ggtatatcgg tcaaaccggc gaccgaggtt gaagcgctgt ttccattact cagcgagctc
      961 gatttggtac ttatcatgtc agttgagcct ggtttcggcg gccagaaatt catgccgaac
     1021 tctctcgaaa agatccgttc gcttcgaaag gagatcgacc ggtgcggcta taaaacacgt
     1081 atccaagttg atggcggaat caaccctgag acagcgaagc ttgtgcgcga tgcaggcgtc
     1141 gatgtgattg ttgccgggac cgcggtcttt cgtgcggatg atccggcgcg tgcgattgca
     1201 gcacttcgcg aaacgccgtg agaaatgaga ctgaatgtgt aaaatgccta caaccgagac
     1261 gcgaataccg ccaaatggat cgacgactca atcgttgacg atcgtcagtg atcggattag
     1321 cggatacgaa gcgacggccg gtaatttgtt cggaggagtc gtcggcagcg tcttgtcgcg
     1381 aatactcggt gctcccggcg tgggaatacc gatggatagc atctattcga ccgcggtgat
     1441 cccatggaga ctctcgcgcg accgcaatcc acggcgtggc gaaatcaatg ggcgccgcga
     1501 ttctctcgcc gcagaactca cgaccgtgca gccgcttgta atcgtcgctc tcggtgatat
     1561 ctcggcgtat gccttattcg cagatcggcc gttcgaacag ggtgaatgga ggacgtggaa
     1621 agcgctcggg ttctccgctc caacattctg cacgttgcat ccgagagaag cgctctggaa
     1681 tcacatcgac gaggggcgct ttcttgccga tcctcgcacc aaaaagcgtc gaatgtggga
     1741 cgattggcgt gctatcgccg atcgctataa caacgtgcgt gaacgtggta cctcgcctga
     1801 cgacggattg gaagaaaatc gatgaaggtg acgatacttg ggagtggtgc cgccgaaggt
     1861 attccggcgt tctattgtcg gtgccgagtc tgccggaacg cggcggaatc ccgtggtcgg
     1921 aatatccgca cgagagcatc ggcacttttc gatgacacgt tgaaagtcga ttacggaccg
     1981 gatacgtacg cacagacgtt gatgcacggc gtcgatatgc gagcggttcg gattatcgtg
     2041 atttcgcacg cacacgaaga ccatttcact ccgactgagt ttatctggcg cgaaaaaaac
     2101 ttcattcaag acggaggacc atatgcagtc gaactctacg gtaatcggac ggttggcgag
     2161 ttattcgatg gactcgcgat tcgatacggg atctctcgcg aggaactctt tcaacgtctc
     2221 agcctaagct actctgaggc caaaccgttt ttagcgatcg acgctgaacc gtacagaatc
     2281 tatccgattg cggcgaccca tgagccaaaa caggttgcgt tgaattacgt gattgaccgc
     2341 aacggtgttc gggtgctcgt cggattcgat accagtcggt accgtccgga aacatgggat
     2401 tacctgaccg agttgtgttg tgacgcaccg ttcgacgcag tgctgatgga ctgtacgatg
     2461 gggaaatatg gtggtggaga tacccacatg ggtatcgaag acaacgaacg tgtgcgcgaa
     2521 gaaatgtata cgctgcggat cgtcggtgaa gcgacacgct acgttcttac ccacatctcg
     2581 cacggcggtg atttcacgca cgacgaactc gccgagcgtg ccaactcgat cggtatgctc
     2641 gccgggtacg acggcatggc gctggttcta taaacgctac gccgatgtgt cctgctacgc
     2701 ttctatctga tcggcagaat gaggaatacc aatacgtccc aaacagcgtg actaacgatc
     2761 cccggccaca acgatttcca acgcagatac atccaacccc aaaacatccc gcagatcaaa
     2821 gccgccatca cgagcatgaa gttccatgac cacacatgaa cgatggcgta cacgaatccc
     2881 gctgcgatcc atccgactcg gtttccccat cgccgggcaa atcgatcttg gagaaaccca
     2941 cgccagaaca cttcttccgc cggccctatg aggaagaaca acagaagacc gatcgtgagt
     3001 ggattcatct gcgtcttcat cgcgtatatt ccgccgactt gatcgacggc gaacgggaaa
     3061 atcgctgcac ttacgaaatc accaacccag aataccacgt acaaaacgac tgctgaaacg
     3121 agcccaattg ccacgtggcc ggcatggaat cggaaaattc gttgaagctg ggagcgcatc
     3181 acgatgattc cggtaattgc gagtatcgtt gccgcgccgg tcatgttcag ccagaaattc
     3241 ccccattcca agccgaacat gacgccccac attacgcctg cagcgatgag agttatcaat
     3301 tcaacggtca tatcagtttt ccgagaacga taccgatcat gagaagaaca ccgaataaca
     3361 agtgcacttg cgcggtcatt tgatcgatta ttgcgaggtc tgatgtatgc gttcctccgg
     3421 cggaacgtat cacgttcact accttcgatg ccggcagaat gctgagggcg acaatgagtg
     3481 tccaaaacgg aagtacacca aacgcgatca tgatgattac gccgatatat gcgctgatga
     3541 ccatcgcgta gtattcaatc gctgcgaatg actgcccaaa ggtgttcgat attgttacaa
     3601 tacctgcagt cgcgtcatca gcgatgtcgc gtaggttgtt tgcttggacg atcgcggcta
     3661 caaggaaacc gacagggagc gatatgaaga acacgtcgcg cgagaatgac cccgtgagga
     3721 cataaaacga tccgataacc atcaacggcc cgaacaacgt gaaaatgacc gtttccccga
     3781 atccgcgata tttgaactcg atcggccaac ctccgtagaa aaaacctcca ataagaccga
     3841 ctatcccaag ccataacaac ggtgttccac gtaacgttac catccaaagc ccgaggctcg
     3901 ttcccgcgag aaaaaggatt agaccgccga aaaggactgc gcgtggtgca aggtacccgt
     3961 cggtgagaac acgcgatccg cccatcgtgc gaaatcggtc gacgccgcgc ttgtagtcga
     4021 aatagtcact tacaaggttt gcgcccgcgt ggtagaacac acccgcagca atgatgaatg
     4081 gcagtaacca ccacgagacc gtatcgactt ggaggtacca ggcgagtacc gctccgagta
     4141 gcatcggtgt taccgacgcc gtatacgcaa acggtcgcga cgcttgccac cacttcgcaa
     4201 ggattccggg cggtcgctga ccgtatggaa actcaaccgt tttcgtaccc gatgtatcgg
     4261 acaaatggat cacatgcggt atgacccgta ccaatcgcgc ggtatcgggg atattagcgc
     4321 caattttgat aaaaaagttc cgtgcttcgg gctcttcaac ggaggttccg agaatgattg
     4381 cgcgtccatt actgctcaat tgttcatcat cggcagtcgc gtcgaattct accgagggat
     4441 cgtggaggat cgctgtgtgc acctctccgc cgtcgtcgat cgcaagatag ataccgtcgt
     4501 ttcctggtgc caccgaaacg ccgagatgct catcaacagt cgatcgaagt gtgatttttc
     4561 ccgcgcttcg gattatccgc tcaattctcc cgatggattc
//
LOCUS       DSCC01000328            3781 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016312, whole genome shotgun sequence.
ACCESSION   DSCC01000328 DSCC01000000
VERSION     DSCC01000328.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016312"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            2530..>3781
                     /locus_tag="ENN56_04075"
     CDS             2530..>3781
                     /locus_tag="ENN56_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74700.1"
                     /translation="MTTRLRLASAVVLVAGVMLAFAPVIEANTAQPPFHMGRVWANPE
                     FDGCGGWGGSAVQVPGGIVQPTVWPSDNALKRGWVGHARKFGNYLVTTNWTDPDGVVN
                     PVSGSYLYRSYNYDAYAVQNWIEGSGGADGVNLNYLYPVGITLKMRNERPNIYVNGNV
                     IDFSNGPWDPEADYEIRSDSGTFPYPPPVIVPDQVVEYTIDSKWRYLQGVEVVRTNIA
                     YPFGSAHQDYYIQDFTLTNNGISGANEPDDPNDPAPVLTGQTITNMIWIWAYGFLNFS
                     AGDYATRSWDNYAQYTDPWGSGNYALYWSDGNAKELVPDVAPFAGPDWGDYPTGPEWE
                     GHPHGNAHIMFGTLFASASGAAPTTSLVGQPAFRVIGAERGLDYAGKAYSPWSATHML
                     ECIASGDYQMPDDTDIRDDSWSGQW"
BASE COUNT          828 a          975 c         1105 g          873 t
ORIGIN      
        1 attcccacga tcctgatatc gccgccgata tcgctatggg ttgggcatta ccgaagaaac
       61 tcggtggtat cgtcctcggc tacagctata accgcgaaat gaccgcggtt ccggcgttgg
      121 ttccgtacta tcgcgatcgg cagttcgaat caaaaatcac cttgacgccg attgacaatc
      181 tcaaactgaa cttcggttat atggtcgctg acagtcgttc gaccggtgga gcggcgggtg
      241 tactggttca gagttctgaa ggtgttgcga cggccggtat accgcgcggt gacgatcccg
      301 tgtcgcttcg gtctccgtct gagctcatca agacgatgaa cgaaaatgac agtgctgaaa
      361 acagcaaggt gaatctgtcg tactcggcgc cgttgaccag tgacttcagc cagatcatgg
      421 gaacgttgac gtatacactg agcccgcaga cgtactttac ggcttcattt ggccgttcgg
      481 aaacgtcgtg gagcctcaag cgcgatttgc cgcgtgccga tatggacccc gaccggggtg
      541 cgttcgacgc cgactcgaag tggagcgcta cttcggactg ggattacggc gcattccttg
      601 gaacaatctt cacgtatatc ggtgacttca cgaagccgcc gagtatgtcc tacgcgacga
      661 atccggacag cttcttgctt cgttcgccgt acgcgcaccc gaacgcgtat cccgcgccgc
      721 cgaaagagac gaagttcgtc accgagacgt ttacgtgggg acctcgttcg tgggtaccgg
      781 cgcgtggtga ccgaaaccca gcgactgacg acacgacatt caacgtgatg atcgtatcgc
      841 cgcaaggttg ggtgggtaac ccgtacggtg atctggcggc tcgattcacg ttgggtggcg
      901 gtggaaagca gatcattgaa tcgagtggca cacagactac cgcgcgtggt gattttaccc
      961 acgtgatcgg ccaacacacc gtcaagaccg gtatcgagta catcggtcgc gatttggaat
     1021 atctccagga agaatcgagc ggactctttg aatccggcgt tggcggcgga cgcaactcgc
     1081 aaatgcgtga cttcggtggg aagtatcccg ctgcgcagcc gaagattatc gggacgtact
     1141 tgcaggacaa gtacgagtcg gatggtatga tcgcgaacct tggttttcgt ttcgagagat
     1201 tcgacgcaag ccatccgacg tggttctact cggatatgtt caacattgtc gtcatgggag
     1261 aagcgaatag ccaggcgata tctcgtcggt tgttcattga agcgggaatc gattcgacgt
     1321 ccaatcttct tggttcaata ggtacgaact tctacatact gaacgacatg ctcgacggaa
     1381 atgctccgat gccgtatgat gtcgtgcgcg cgtggccgtc cgatgataac aaagtccatt
     1441 ggcgtgttgc accgcgtttc gggatttctc atccggtgag taatcgtacg aagttcttct
     1501 tcaactacgg cgtcttctac tcgatgcaga agccgatcca tatgtatgga tacaacatcc
     1561 atgatggacg gcccggcggc ggtagcggac gtatcgaata catttacaac ccgagcctcc
     1621 gtccgagcac cacgacgatg tatgaggttg gtgtcgagca tgttctacca cgggacattg
     1681 tgttcaaggt ctccgggtat gcgaaataca acgaagacca agtcacgatg gtcacggtca
     1741 actactcgaa cttcacgtat cgcgtgtatc gaaacgcgaa ttacgaagat gtacgtggat
     1801 acgagataca acttgcacgg aacaccgggc gttttgtgaa cggacaagtc acgtacgagt
     1861 tatacagttc acgtacgggc gaagtgtcga taccgaggat tggtgacaag atgtcgtatt
     1921 tcttgacacc gtatctgccg accgtccgac gggttcccgc gcgaggcttc ttccgtgcgt
     1981 ttattcgact cggcacgccg atggactggg gcacaatgtc cggtggttgg tcgctcggta
     2041 cggaatacgc atggcaaaaa ggcgcggagt atgtctacaa tccgaactta ctcccagcgc
     2101 gcgagttgac tgaagagaac tatttgcctg gtgtggcgta ccataacgtg aacatgaagt
     2161 tttcgaagcg attcgccacg cggggtggta ggttcgtgac ggcgtacttt gatatactta
     2221 acgtcttcaa ccggaagaac ctcaattcga acggtgtgaa gaactggcag gattacttga
     2281 actacatcta cacccggaga tctctgggcg aagatattga ggttggagac aagtcgacat
     2341 tttatgtttt caccgaaccg tataagatcg atccggacgc agagttgtgg tcgcgtccga
     2401 tcagcccgga gaccgattgg attcaattcc cgaatccgcg tttctaccgg tttggtttgc
     2461 ggttcgaaat atgagggtgc gcaccgtgcg taccctttat cacgtcgtta acgttcaagg
     2521 aggaacgaca tgacgacacg attgcgtttg gcgtcagcag tagtgctggt tgccggtgtg
     2581 atgttggcgt tcgcgccggt catcgaagcg aacaccgcac agccgccatt ccacatgggt
     2641 cgcgtttggg caaacccgga gtttgatgga tgcggtggtt ggggcgggtc ggctgttcag
     2701 gtcccgggtg gtattgtcca gccgaccgta tggccgtcgg ataacgcact taagcgtggc
     2761 tgggtaggcc atgcgcgtaa gtttggtaac tatctcgtca cgacgaactg gacggacccg
     2821 gacggagtgg ttaaccccgt atcgggttcg tatctgtatc gttcgtacaa ctacgatgcc
     2881 tacgcggtgc agaactggat cgaaggttcg ggcggcgctg acggcgtcaa cctcaactac
     2941 ctctatccgg ttggcatcac gttgaagatg cggaacgagc gtccgaatat ctacgtgaac
     3001 ggaaacgtta tcgacttctc gaacggtccg tgggacccgg aagccgacta cgaaatccgt
     3061 tccgatagcg gaacgttccc gtatccgccg ccggtgatcg tgcccgatca ggttgtcgag
     3121 tacacgatcg attcgaagtg gcgttatctt cagggtgtcg aagtcgttcg cacgaatatc
     3181 gcgtatccgt ttggcagtgc gcaccaggat tactacatcc aggacttcac gctgaccaac
     3241 aacggtattt cgggtgcgaa cgaaccggac gatccaaacg atccggctcc ggtgcttaca
     3301 ggccagacga tcacgaatat gatctggatc tgggcgtatg gtttcctgaa tttcagcgcg
     3361 ggtgactacg ccacgcgctc ttgggataac tacgcgcaat acacggatcc gtggggctca
     3421 ggtaactacg cactctattg gtcggatggc aacgcgaagg aactcgttcc cgacgtagcg
     3481 cccttcgccg gtccggattg gggtgattat cccacgggtc ctgaatggga aggtcatccg
     3541 cacggcaacg cgcatatcat gttcggtaca ctgttcgcct ctgcgtcggg tgcggcaccg
     3601 acgacttcgt tggtcggtca gcccgcattc cgtgtgatcg gtgccgagcg tggtctcgat
     3661 tatgccggta aagcgtattc gccgtggagc gcaacgcata tgttggagtg catcgcttcc
     3721 ggtgactacc aaatgcccga tgacactgac atccgagacg attcgtggtc tggtcagtgg
     3781 g
//
LOCUS       DSCC01000329            2331 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031218, whole genome shotgun sequence.
ACCESSION   DSCC01000329 DSCC01000000
VERSION     DSCC01000329.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031218"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            19..1044
                     /gene="nadA"
                     /locus_tag="ENN56_04080"
     CDS             19..1044
                     /gene="nadA"
                     /locus_tag="ENN56_04080"
                     /inference="COORDINATES: protein motif:HMM:PRK09375.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDS74701.1"
                     /translation="MTNLTILDLPPTKADLLDLESAPSAAEMKAYATENNILILAHSY
                     QDGEIQDSAHIVGDSLELARAASELDAPNICFCGVHFMAETAKILNPESNVFMPDPEA
                     GCSLAEGCKAEDLEQYQAFLQKKLGRDDLVTICYINTTAAVKALCDYVCTSGNSLEVI
                     DAIPADSPILFVPDVHLGGWVKEQRPDRDIHLWDTACIVHELFSIETLLDLKHQYPDA
                     ITLAHPECPKNIRTESDYVRGTAGMLRIVKEAHGKQFIVATEGNMIYRLKTEAPDNEY
                     IAAPGASCACNLCPHMQRTTVDKLWHSLRTKQFEITVPDDVAKAARVSVDRMLAIKPK
                     RETVVRD"
     gene            1306..>2331
                     /locus_tag="ENN56_04085"
     CDS             1306..>2331
                     /locus_tag="ENN56_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HDS74702.1"
                     /translation="MQYRKLWIGFAAVIIVSFSVLGYFGVEIYREAPPVPERVITDDG
                     VLLFTGQNIKDGQNVWQSMGGQQVGTIWGHGAYIAPDWSADWLHREAEFIVNQWAVRD
                     FERPFDELDNERQAMLQARLQRELRENRYNPNTKTLTVSPLRAEAIAAAGAHYTSLFM
                     NDPELSDLRKAYAIPENAIRDPERMEWMNSFFFWASWAAVTNRPNDTKTYTNNWPPDD
                     LVGNRPTGWLLLWTGFSVIVLIAGIGVLATYYASHRDDDVNPTEIPDRDPLLGLKATP
                     SMKATLKYFWIVTALILAQIGFGVITVHYGVEGDGLYGIPLAEWLPYSITRTWHVQLA
                     IFWIATSW"
BASE COUNT          558 a          654 c          611 g          508 t
ORIGIN      
        1 agaggttgaa cggaaaatat gacgaatctt acgatacttg atctaccgcc gacgaaggcg
       61 gatcttctcg accttgagtc cgcaccgagc gccgctgaaa tgaaggcgta cgcaacagag
      121 aacaacattc tcatcctcgc acacagctac caggacggcg agattcaaga ctccgcgcat
      181 atcgtcgggg actcacttga gttggcgcga gcagcgtcgg aactggacgc accaaatatc
      241 tgcttctgcg gcgtccattt catggcggaa acggcgaaga tcctgaatcc cgaatcgaac
      301 gtattcatgc ccgatccgga agcaggatgt tcacttgcgg aaggatgtaa ggcggaggat
      361 cttgagcagt atcaagcgtt tctgcaaaag aagctcggtc gcgacgatct cgtcaccata
      421 tgctacatta acaccactgc cgcggtaaaa gcgctctgcg actacgtctg caccagcggt
      481 aactcgctcg aagtaatcga cgcgattcca gcggattcgc cgatactatt cgttcccgat
      541 gtgcatcttg gcggatgggt gaaggagcaa cgcccggacc gcgatattca cctttgggac
      601 accgcatgta tcgtgcatga gttattcagc atcgagacgc tgttggacct caaacaccag
      661 tatcccgacg cgattacgct tgcgcacccg gaatgcccga aaaatatccg tacggagtcg
      721 gattatgtcc ggggaaccgc gggtatgctt cgaatcgtca aggaggcgca cggtaagcaa
      781 ttcatcgtgg ccaccgaagg gaatatgatc taccggttga aaacagaagc gcccgacaat
      841 gagtatatcg cggcacccgg tgcgtcctgc gcgtgcaatc tctgcccaca catgcaacga
      901 accactgtcg ataaactctg gcactcactg agaacgaagc aattcgagat aacagtgccc
      961 gacgatgttg ccaaggcggc acgagtttcc gtcgaccgta tgctcgcaat caaaccgaaa
     1021 cgtgagaccg tcgtacgcga ttagacttaa ggcacgccgc acaagtttcc cctacgcgac
     1081 tacgtaccat tattgatacg tagtcgctcg catcacgcgt ccaatcgttt ccccgcagag
     1141 ctcttcacct tccgtgaaaa aaacctcgat cggatcttct tgacattggg ctgtttgact
     1201 ctcatataga tgaagatgta tttatatcgg cgttgtgtcc gatttctcgt tttccgtacc
     1261 acctcttctc gaaagcacgc caccacgcgt aggaggagat caccgatgca ataccgcaaa
     1321 ctatggatcg ggtttgccgc agtcatcatc gtttcgttta gcgtactcgg ctatttcggt
     1381 gtggagatct accgtgaagc gccgccggta cccgaacgtg tcataactga cgatggtgta
     1441 ctgctcttta ccgggcagaa catcaaggat gggcaaaacg tctggcaatc gatgggtgga
     1501 cagcaggtag gtactatctg gggacacggt gcgtatatcg ctcctgattg gtccgctgat
     1561 tggctgcacc gcgaggctga atttatcgtc aaccaatggg ccgtacgaga tttcgaacgg
     1621 ccgttcgatg agcttgacaa cgaacgtcaa gcgatgcttc aggcacgtct ccagcgcgag
     1681 ctccgcgaga atcggtacaa tccgaacacc aagacactca ccgtatcacc gctccgcgcc
     1741 gaagcgattg ccgctgcagg tgctcactac acatcgctgt tcatgaacga tccggagctg
     1801 agcgacttgc gaaaagcgta tgcaattccc gaaaacgcga ttcgcgatcc cgagcgtatg
     1861 gagtggatga actccttctt cttctgggct tcgtgggcag ccgtcacgaa ccgtccaaac
     1921 gacacgaaaa cgtacacgaa caactggccc cccgacgatc ttgttggaaa tcgtccgacc
     1981 ggctggttgt tgctgtggac gggattcagt gtgatcgtgc ttatcgccgg tatcggcgtc
     2041 cttgcgacgt attacgcctc tcatcgcgat gatgacgtaa acccgacgga gatacccgat
     2101 cgcgatccgc tcctcggatt gaaggcgacg ccatcgatga aggcgacgct gaaatacttc
     2161 tggatcgtaa ccgcactcat tctcgcacag atcggattcg gcgttatcac ggttcattac
     2221 ggagtcgagg gtgacggtct ctacggcatt cctctcgccg aatggcttcc atactcgata
     2281 acgcgaacct ggcacgtgca actcgccatc ttctggatcg caacctcatg g
//
LOCUS       DSCC01000330            2063 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036936, whole genome shotgun sequence.
ACCESSION   DSCC01000330 DSCC01000000
VERSION     DSCC01000330.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036936"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1721
                     /locus_tag="ENN56_04090"
     CDS             <1..1721
                     /locus_tag="ENN56_04090"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74703.1"
                     /translation="DDGSGNDTVGRPLLDRGWSEDGNRFSFAVIGDKTGGGQENWPIF
                     DTAIEEINRLRPAFSIMVGDLIQGYHGDTVKVAEEWEEFRSHVGKLEVPFFALPGNHD
                     VTTPELMEYWGRHIGPYYYSFDYLGAHFLVLNTEQERFSGVWGFGDAQMRFALDDIAR
                     NARARHTFILMHKPAWLNELYTSEWAQIEAALGDRAYTVFAGHRHNLSYEVRNGRRHF
                     VVGPTGGGTRESAVRELGAFHHVTWVTVEDDSVHIAIIEPGGPILPEDIATAEFKQNA
                     RHLVRFAPRVPTQSRQGTLNPTLDVHFRNLLPDTAAVTLSFSDLADASREHTGWTLVD
                     AIDSVHVLLPPLTRDSVTVHFTTPTDRLHRVPQARVSTSYRGTPMFALSSPVPVFPEA
                     AIRRVEAWRVIGPFPNTDNREQFTYTPSAEPLLRDILGPNGMFDPEASIELPFANDAE
                     PEIAQWEVIESNARGNVDLGAFYRTTERAVAYLTVGIESPDARKTWAVMWNDDYARVM
                     LNGEMVRGGRVFSGDQRGPDYIALDLREGMNVLVVKAVNTGNAWSTSLRIVDPDGELT
                     FTTEAQ"
     gene            1813..>2063
                     /locus_tag="ENN56_04095"
     CDS             1813..>2063
                     /locus_tag="ENN56_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDS74704.1"
                     /translation="MTEPIRLPDLPPFSADSGMISLDRTSDGRFAVGRAGVRAVVATG
                     DRKVEFVAYAEHTLALVTSALGYPAYYPVHPVAVERPVKA"
BASE COUNT          423 a          617 c          615 g          408 t
ORIGIN      
        1 ccgacgacgg aagtgggaac gacaccgttg ggaggccgct gctcgatcgc ggatggtccg
       61 aggatgggaa tcgcttcagc ttcgcggtca tcggtgacaa aaccggcggc ggtcaagaga
      121 actggccgat cttcgacacg gcgattgaag agatcaatcg gcttcgtccc gcgttctcaa
      181 tcatggtcgg cgacctcatc caaggctacc acggcgatac ggtcaaagta gcagaggaat
      241 gggaagagtt tcggagccac gtaggcaagc tcgaggtgcc gttcttcgca cttccgggaa
      301 accacgacgt cacgacgcct gaactcatgg agtattgggg gcggcatatc ggcccgtatt
      361 actattcgtt cgactacctc ggcgcgcatt tcctcgtcct gaacaccgag caagaacgct
      421 tcagtggcgt ttggggcttc ggcgacgcgc agatgcggtt cgcactcgat gatatcgcgc
      481 ggaatgcaag agctcgccac acattcattc tcatgcataa accggcatgg ctcaacgagt
      541 tgtatacgag tgaatgggcg cagatcgaag cggcactcgg cgaccgtgcg tataccgtgt
      601 ttgcaggaca tcgacacaat ctgagttatg aggtgcgtaa cggtcgcagg cacttcgtcg
      661 tgggtccgac cggtggggga actcgtgaga gcgccgtgcg agaacttggc gcgtttcatc
      721 acgttacgtg ggtgaccgtc gaagacgact cggtgcacat cgcgatcatc gaaccgggcg
      781 ggccgatctt gccggaagac attgcgaccg ccgaattcaa acaaaatgct cgccatctcg
      841 tacgctttgc gccgcgcgtc cccacccaat cgcggcaagg cacgctcaac ccgacgctcg
      901 acgttcactt ccggaatctg cttcccgaca ccgccgcggt cacgctctcg ttttccgatc
      961 tcgccgatgc aagccgcgag cacaccggat ggacactcgt cgatgcgata gactcggtgc
     1021 atgtactact gccgccgttg acacgcgact cggtcacggt tcacttcaca acgccgacag
     1081 atcggttgca tcgcgtccca caggcacgcg tctccacgtc gtatcgcggc acgccaatgt
     1141 ttgcgctgtc gagtccggtg ccggtcttcc ccgaagccgc aatacgtcgc gttgaagcat
     1201 ggcgcgtgat tggcccattc ccgaataccg acaaccgcga acagttcacc tatacgccaa
     1261 gcgccgaacc gctgctccgc gatatcctcg ggcccaacgg gatgttcgac cccgaagcga
     1321 gtatcgagct cccgttcgca aacgacgcgg agcccgagat cgcgcagtgg gaggtcatcg
     1381 aatcaaacgc acggggaaat gtcgatctcg gtgcgtttta ccgaaccacg gagcgcgccg
     1441 ttgcgtacct gactgttggc atcgaaagcc cggacgcgcg gaagacatgg gcggtgatgt
     1501 ggaatgatga ttacgctcgc gtgatgctga acggtgagat ggttcgcgga ggtcgcgtct
     1561 tttccggcga tcagcgtgga cccgattata tcgcgctcga tctgcgcgaa ggaatgaacg
     1621 ttctcgtcgt gaaggccgta aataccggca acgcgtggtc gacaagcctc cgtatcgttg
     1681 atccggatgg cgaactcacg ttcacgacgg aagcgcagta aacgacacgc gctgtacgag
     1741 gaaagcaccg ttcgtgcttg ccgatctttt tgtaaaacgg ataccgttga tgcggtatgt
     1801 aaaggacatc tcgtgactga accgattcgt cttcccgatc tcccaccgtt ttccgccgat
     1861 tccggcatga tctcgctcga tcgcacgtcc gacgggcgat tcgctgttgg gcgcgccggc
     1921 gtgcgcgcgg ttgtggcaac cggcgaccga aaggtcgaat tcgtcgcgta tgccgaacac
     1981 accctcgcgc tggtcacatc ggcacttggg tatcccgcat actaccccgt gcatccggtt
     2041 gccgtcgagc ggccggtgaa agc
//
LOCUS       DSCC01000331            2846 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024039, whole genome shotgun sequence.
ACCESSION   DSCC01000331 DSCC01000000
VERSION     DSCC01000331.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024039"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            111..434
                     /locus_tag="ENN56_04100"
     CDS             111..434
                     /locus_tag="ENN56_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74705.1"
                     /translation="MDPHRQHIRANASGELYLEDGSGRTTRLYRFREALDRATVSAST
                     YYLWVKRGIVPDTRLRDRGKWRVFTESELRRLIATARQEGHHTAPISSWEEALAESQD
                     SDWRE"
     gene            434..1654
                     /locus_tag="ENN56_04105"
     CDS             434..1654
                     /locus_tag="ENN56_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74706.1"
                     /translation="MRVKGIFTSRWTAALVATIALSAVFGCEARMTSSDTQRFNPYSA
                     PDLEVLVLDMNDAAVVGANVSLYERAGDGAHRVGKGETDANGIVTFVNPAMGEYLLYA
                     RKEGYIGTSAFAQVQRTGVVVGTLRMRPIASLADASVGMIDQSGGTITTVSVPTLPQS
                     AITFDGSSFTGSTTVTIATVANNQIPSPPPGQIALGAIVIESTNPLSGSASVAVGLPF
                     DLPAGTSIPLFAYNDITGEWMQVAVGTVDPITGQVVAQIDDIALVSALAGFVPHRVSA
                     TISTPVGPPRTLGPGDPVEIRLVYTPTLVFPEGTNYTVKTQEWLKGVMGSIIGQEFGR
                     EVTLTVTRDPSTIQTYWVYDNIDDYIISINVGGSKPAAVAKVAASYQFQNRSVTRSQE
                     LRAVEHNSGTGGGS"
     gene            1699..2742
                     /locus_tag="ENN56_04110"
     CDS             1699..2742
                     /locus_tag="ENN56_04110"
                     /inference="COORDINATES: protein motif:HMM:PF03417.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74707.1"
                     /translation="MTGTLVSLYHSSMQPPDMIQSGTRREIGFAQGARIRSLRLPPIG
                     DADRSFGAQCRRSVERWHLPMIEEYEGMLSASWLPEDAFESYYFGRTAPYAGGCTNIA
                     VTGESTVSGTSLVGRNYDWAYADKRWCETRVIAPIGEPRRVGYTHHWGGLCDAMNEHG
                     VTICIASLPPTGRTAPGLQWHIVVDLVMTRSTSVAEAVDLLVGIPHVRSLSYLIADAT
                     DARLVEAGPDGSTVNSPEHGLLVGTNHRIGNGSIDDPRRDTSRKRRERARELLVGKID
                     RSDMVRVLSDHTGGICAGAYDFEQRVHTTSGQSGTIWSMIASPADRLFEIAPGHPCST
                     PFNGIRWIGYGEQ"
BASE COUNT          608 a          809 c          846 g          583 t
ORIGIN      
        1 attcgaagga taccattatt gagttgcggc ccgcgatgac gattaaggtg agcacatgcg
       61 ctcccaacat cgtcgggtgt tcttgcgtgc cggctttgga gacaacatac gtggacccac
      121 acaggcaaca catacgggcg aacgcttcgg gtgaattgta tctcgaggac gggtccggac
      181 ggacaacgcg cctgtaccgg tttcgcgaag cactcgaccg cgcaacagtg agcgcctcca
      241 cctattacct gtgggtcaaa cgtggcatcg tgcctgatac gcggcttcgt gatcgtggta
      301 aatggcgagt ctttacagaa tcggagttac ggcgtctgat agccacggct cggcaagaag
      361 ggcatcatac cgccccgatt tcgtcgtggg aagaggcgct tgcggagtcg caagactccg
      421 attggaggga ataatgcgcg tgaaagggat atttaccagc cgatggactg cggctctcgt
      481 agcgacaatc gcgctgagcg cggtgttcgg ctgcgaggct cgtatgacca gttcggacac
      541 gcagcgtttc aacccataca gcgcgcccga tctggaagtg ctcgtattgg atatgaatga
      601 cgcagcggtc gtaggcgcga acgtgtcgct ttacgaacga gccggtgatg gagcgcaccg
      661 tgtcggaaag ggcgaaactg acgcaaacgg aatcgttaca ttcgtcaacc cggccatggg
      721 tgagtacctc ttgtatgccc ggaaagaagg ctacatcggt acatcggcgt ttgcgcaggt
      781 ccagcgtacc ggcgtggttg tcggcaccct tcgcatgcgg ccgatagcaa gcctcgccga
      841 tgccagtgtc ggtatgatcg accagagcgg cggtacgata actacagtgt cggttccgac
      901 acttccacag tccgcgataa ccttcgacgg aagctcgttc accggttcaa cgaccgtcac
      961 catcgccacc gttgcaaaca accagattcc ctcaccaccg ccggggcaga ttgctcttgg
     1021 cgccatcgtt atcgagtcga caaacccgct ttccggctcg gcaagcgttg cggttggatt
     1081 gccattcgat cttcctgcgg gtacttcgat tccactgttc gcgtataacg acataaccgg
     1141 tgaatggatg caagtcgccg tcggcacggt cgatccgatt accggccagg tcgttgcgca
     1201 aatcgacgat atagccttgg tgagtgcttt ggcggggttc gtgccacacc gagtatccgc
     1261 aacgatatca acaccagtgg ggccgccgcg cacacttggc ccgggcgatc ccgttgagat
     1321 acggctcgtt tacacgccaa cgcttgtttt cccagaggga acgaactaca cggtgaagac
     1381 gcaggagtgg ctcaagggtg tcatgggaag cattatcggc caggaattcg gacgcgaagt
     1441 gacccttacc gtgacgcgtg atccgtcgac gatccagacg tactgggtgt atgataacat
     1501 tgacgactac atcatttcga tcaacgtcgg cggaagcaag ccggctgcag tcgcgaaagt
     1561 ggcagcgtcc tatcaattcc agaaccgctc cgtgacgcgg agccaagagt tacgcgccgt
     1621 cgagcacaac tcgggcacgg gcggcggttc gtaatcgccc catagacaag cgtagtatcc
     1681 cgagggtgct ggttctcgtt gaccggcacc ctcgtttcgt tgtatcactc ttccatgcaa
     1741 cctccagata tgattcagtc aggaacccgc cgcgaaatcg gatttgcgca gggcgcgcgc
     1801 atccgttcgc tccgccttcc tccgatcggc gatgcagacc gatcgttcgg ggctcagtgt
     1861 cgtcgttccg tggaacgatg gcatcttccg atgatcgaag aatatgaagg gatgctgagc
     1921 gcaagctggc ttccggaaga cgcattcgag agctactatt tcgggagaac ggctccatat
     1981 gccggaggtt gtacgaatat cgccgtgacc ggtgagtcaa ctgtgtcggg cacttcgctc
     2041 gtcggtcgga actatgattg ggcgtatgcc gacaagcgat ggtgcgagac gcgggttatt
     2101 gcaccgatcg gcgaaccgcg ccgagttgga tacacgcacc attggggtgg cctctgcgac
     2161 gcaatgaatg agcacggtgt tacgatctgc atcgcatcgc ttccaccgac aggccgcacc
     2221 gcgccgggcc ttcagtggca catcgtcgtc gatctggtga tgactcggag caccagcgtc
     2281 gccgaagccg tcgacctcct cgtaggaatc ccgcacgtgc gttcgttgag ttatctaatt
     2341 gcagacgcga cggacgcgcg actcgttgaa gccggtccgg atggtagtac cgtcaactca
     2401 ccggaacacg gacttctcgt tggcacgaac catcgaatcg gtaacggttc aatcgacgat
     2461 ccacgtcgcg acacatcacg aaaacggcgt gaacgtgcga gggagctact ggtcggcaag
     2521 atcgatcgaa gcgacatggt gcgggtgctg tccgatcata cgggtgggat ctgtgccggc
     2581 gcctacgact tcgaacaacg cgtccataca acaagcgggc agtccgggac gatatggtca
     2641 atgatcgcgt cgcccgcgga tcggttattc gagatcgcac cgggacatcc gtgttcaacg
     2701 ccgttcaacg gcatacgttg gataggatac ggagaacagt agcgttatcg cgtgcccgtc
     2761 gcaccccggt ttttgatgtt ctgatcccaa tagcccatcc acgtatcgcg attcgctcca
     2821 acaacgcccc aatcgacgtg gagctc
//
LOCUS       DSCC01000332            3164 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020789, whole genome shotgun sequence.
ACCESSION   DSCC01000332 DSCC01000000
VERSION     DSCC01000332.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020789"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(160..1224)
                     /gene="mltG"
                     /locus_tag="ENN56_04115"
     CDS             complement(160..1224)
                     /gene="mltG"
                     /locus_tag="ENN56_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDS74708.1"
                     /translation="MTRSRGTRIIGWTITLTAVAILGGAAVYAGVSWLNTSPSRVELY
                     SLEIEPGMSAGRVADKLQREGLIRSARFFRVLARFHGFDRQIHAGTHWVDGRKTTSGI
                     LTQLLSGGIRIVRVTVPEGLVIDDIAELLAKELPFEPAEFVEACQDSELVATLAYPGA
                     QTMEGYLFPDTYFFDVNADVRETIGVMSQRFHNVFADSLVLRADTLRMSVHEIVTLAS
                     IIEKEAQVRSEQPIISQVFHKRLKIGYRLGADPTVKYAMGQANRRLSLRDIEIDSPYN
                     TYKHRGLPPGPICSPGRGALHSALYPANTDYLYFVANWDGTHTFTRTLRDHNRAKAVS
                     EQRYWEMRREQRRREAEQGG"
     gene            complement(1245..1451)
                     /locus_tag="ENN56_04120"
     CDS             complement(1245..1451)
                     /locus_tag="ENN56_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74709.1"
                     /translation="MSARKYLRVAIEDVLLLSLLIPLAVVFQLVLFVAEGYYRLRRSG
                     VLAPSVVIVAVLAFGIRAIRRTKH"
     gene            complement(1448..1894)
                     /gene="ruvX"
                     /locus_tag="ENN56_04125"
     CDS             complement(1448..1894)
                     /gene="ruvX"
                     /locus_tag="ENN56_04125"
                     /inference="COORDINATES: protein motif:HMM:PF03652.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDS74710.1"
                     /translation="MRALAVDYGDRRVGLAISDELGIAAHGLDTLDVVSPKRAANAVV
                     AIANERGAERIVVGMPYAMDGSRGPRAEATEEFCELLRRRTNLPIEEFDERFTTKRAE
                     HILREMGIDERRGRGKKDRIAAVFILQDYLSTLQMRTKTTPESDEQ"
     gene            complement(1891..2490)
                     /locus_tag="ENN56_04130"
     CDS             complement(1891..2490)
                     /locus_tag="ENN56_04130"
                     /inference="COORDINATES: protein motif:HMM:PF00293.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDS74711.1"
                     /translation="MTPFPLPGRFVQRAGEPHPDGTVAFRRPEYRYCPLCATPLEDRG
                     TDHNDEVHPTCPKCGYTAYYNPAPAVGAVILREANGAPEILLVRRRFAPKRGLWSLPS
                     GFMDFGERQIDALAREVAEETGLTLTSASLLSVEDASEDPRTHALLIAFIAHEWSGEP
                     RPGDDASEIGWFSIDALPPDMAWKNHARVLAAVREVLAG"
     gene            complement(2568..>3164)
                     /locus_tag="ENN56_04135"
     CDS             complement(2568..>3164)
                     /locus_tag="ENN56_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HDS74712.1"
                     /translation="DEDGIAENTIVIYTSDQGFFLGDHGWYDKRFMYEESLRMPFVIR
                     WPAVIKPGSVADAMTLNVDFAPLFLDAAGIEVPADMQGRSFLPMLRGETPTDWRDAMY
                     YRYWMHKSHHNVYAHYGVRTHRYKLIYFYNAALGQPDAVDETYEPEWELFDLERDPYE
                     LCSVYDDPAYSDVRRELTDLLHRLQEEVDDERYEKDVD"
BASE COUNT          602 a          964 c          930 g          668 t
ORIGIN      
        1 cctatcgtat tataaagaag ggtatcgcat acgcgacgtg gttacccgcg ctgaccgaaa
       61 gaccggtcga cgacccttcg acagagttcc cccatcagcc gacgggctga gcgaatacga
      121 ccccgaacgg tactgcccta ccgttcgggg tcttcgtgtt tatccaccct gttcggcttc
      181 gcgacggcgc tgctcgcgac gcatttccca ataccgctgc tcgcttaccg ccttcgcgcg
      241 gttgtgatcg cgcaacgtcc tggtaaaggt gtgcgtgccg tcccagttgg caacgaaata
      301 gaggtagtcg gtatttgcgg gatagagtgc ggagtggagc gcgccgcggc ccggactaca
      361 aatcggtccc ggcggcaatc cgcggtgctt gtaggtgttg tacggcgagt cgatttcgat
      421 atcgcggagg ctcaaacgac gattcgcctg tcccatggcg tatttcaccg tgggatcggc
      481 gccgagtcgg tatccgatct tcagccgctt gtggaatacc tgcgagatga tcggctgctc
      541 cgatcggacc tgtgcctctt tctcgatgat gcttgcgagt gtgacaatct cgtggacgct
      601 cattcgaagc gtatccgcgc gaagaaccag cgaatccgcg aacacgttgt gaaaacgttg
      661 cgacatgacg ccgatcgttt cgcggacatc ggcgtttacg tcaaaaaaat acgtgtccgg
      721 gaagagatat ccttccatgg tctgcgcgcc gggatacgcg agcgtcgcca cgagctccga
      781 atcctggcac gcttcgacga attccgccgg ctcaaatggc aactctttcg cgagcagctc
      841 ggcaatatcg tcgataacca gaccctccgg gacggtgacg cgcacaatgc ggatgccgcc
      901 tgagaggagc tgtgtgagga tgccgctcgt tgtttttcga ccgtcgaccc agtgcgtacc
      961 cgcgtgaatc tgtcgatcaa acccgtggaa ccgcgcaagt acgcggaaga atcgcgctga
     1021 acggataaga ccttcccgtt gcaacttatc ggcaacgcga ccggccgaca tccccggctc
     1081 gatttcgaga ctgtagagct ccaccctgct tggcgacgtg ttcagccaag acacgcccgc
     1141 atacaccgcc gctccgccaa gtattgccac ggcggtgagc gttatcgtcc acccaatgat
     1201 tcgtgttccg cgcgatcgtg tcatggatat atcgtatctc gaaatcagtg tttcgtccgg
     1261 cggatggctc gtattccaaa ggcaagcacc gcgacaatga cgacgctcgg tgcaagcacg
     1321 ccactacggc gcagtcggta gtatccctca gcgacaaaca agacgagttg aaacaccacc
     1381 gcgagaggaa tcaggagtga gagaagaagt acgtcctcaa ttgcgacgcg aaggtacttg
     1441 cgcgcactca ttgttcgtcg ctctccggtg tcgtcttcgt tcgcatttgg agcgtcgaaa
     1501 gatagtcctg aaggataaac actgcggcga tacggtcttt cttcccgcgt ccgcgacgtt
     1561 catcgatgcc catttcgcgc aggatatgct cggcgcgctt tgtggtgaat cgttcgtcga
     1621 actcttctat cggcaggttc gttcgtcggc ggagaagctc gcagaattcc tcggtcgctt
     1681 cggcgcgcgg tccgcgcgac ccgtccatcg cgtacggcat ccccacgacg attcgctctg
     1741 cgccgcgctc gttggcgatg gcgacgacgg cattcgctgc gcgttttggc gagacgacgt
     1801 cgagcgtgtc gagaccgtgc gcggcgatac ccagttcgtc cgagatcgcc aagccgactc
     1861 gacggtcgcc ataatccacc gcgagcgccc tcatccggcg agtacctccc gtactgcggc
     1921 gagcacccgt gcgtggttct tccacgccat gtccggcggt agcgcatcga tggagaacca
     1981 accgatctcg ctcgcgtcgt caccagggcg cggttctccg gaccactcat gtgcgataaa
     2041 cgcgatcaac agcgcatgcg tgcgcgggtc ttccgacgcg tcttccacac tgagaagaga
     2101 tgcgctcgtc agcgtcagcc ccgtctcttc ggcgacttcg cgcgccagtg cgtcgatctg
     2161 ccgttcgccg aaatccatga accccgaggg gaggctccac agaccgcgtt tcggggcgaa
     2221 tcgacggcga acgagcagga tttccggcgc gccgtttgcc tcgcggagaa tcaccgcgcc
     2281 gactgcgggt gcgggattgt agtacgccgt gtatccgcac ttcgggcagg tcggatgcac
     2341 ctcgtcgttg tgatccgttc cacggtcctc aagcggtgtc gcacagagcg ggcagtagcg
     2401 atactccggc cgtcggaacg cgacggttcc atccggatgc ggctcaccgg cacgctgcac
     2461 aaatcgtccc ggaagtggga atggcgtcat atggtgttgc tccgtcacgc gtcgttttca
     2521 tgttgaatcg gtcgtgccgt gatctcacag gcggcacgat accgcgatta atctacgtcc
     2581 ttttcgtatc gctcgtcatc gacttcttcc tgtaaacgat ggagcaggtc ggtaagttcg
     2641 cgccgcacgt ccgagtacgc aggatcgtca tagacactgc atagctcgta cggatcccgt
     2701 tcgaggtcaa acaactccca ttccggctcg tatgtctcgt ccacagcgtc gggttggccg
     2761 agtgctgcgt tgtagaagta aatgagcttg tatcggtgcg tccggacgcc gtagtgcgcg
     2821 tacacattgt ggtgcgattt gtgcatccag taacggtagt acatcgcgtc gcgccagtcc
     2881 gtcggcgtct cgccgcgcag catcggtaag aagctgcgcc cctgcatgtc ggccggaacc
     2941 tcgatgcctg ctgcatcgag gaaaagcggt gcgaagtcga cgttcaacgt catggcgtcg
     3001 gctacgcttc ccggcttgat caccgccggc caccgaatca cgaacggcat gcgtagcgat
     3061 tcctcgtaca tgaatcgctt gtcgtaccag ccgtgatcgc cgaggaagaa tccttggtcg
     3121 gacgtgtaaa tgacgatcgt gttctccgcg ataccgtcct cgtc
//
LOCUS       DSCC01000333            2034 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1037649, whole genome shotgun sequence.
ACCESSION   DSCC01000333 DSCC01000000
VERSION     DSCC01000333.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1037649"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            258..1547
                     /locus_tag="ENN56_04140"
     CDS             258..1547
                     /locus_tag="ENN56_04140"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS74713.1"
                     /translation="MRIEFMKKSLSPLSIHGGEPAAKNVVFRKWPATTKEDEALVLAS
                     LRQDSHAFGPHADMLQKEFAAWNGNRFCLATNAGTAALHMCIAACDVRPGDEVITTAL
                     SWTSSATAIIHHNGIPVFVDVDWDSMCMDPAKIETAISEKTKAILVVHYWGVSCDMDP
                     IMDIAKRHNLKVIEDACQAHGALYNGRKVGTMGHISAFSLNQNKNLCGGEGGLLVTDD
                     ENLLERARAVWSFGDMRPADSGRDYHNYGLGYMYRTSDLAAAFALSGLRRLDETNRHA
                     QANWKRLDDALIGTPNLVRSWSTPERPTNGYAYVLRVDPAYAKQRGVTLRAMTEGIVA
                     ALKAEGVNTSIANWLLPAHAVFQAKNAYGNGEPWASHARPEVSYALDQFPVAQQCIDT
                     CLWGINNHRPPNGPEQIDALAAAIRKVFTHLDDVPVA"
     gene            1610..>2034
                     /locus_tag="ENN56_04145"
     CDS             1610..>2034
                     /locus_tag="ENN56_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74714.1"
                     /translation="MRVGTAYRDITPDRPLALQGQMHIRIAKYTRDPLTVNAIVFEEG
                     GKRVALISTDVCVLPNHLAEEIKRAVSEVSGIPVQDIIPAATHTHVGPCTTTRLFGDI
                     DPTWREHLIKSTADAVKSAIDDLEDAELFTGAGWLDHMG"
BASE COUNT          458 a          588 c          596 g          392 t
ORIGIN      
        1 actcgaatcc caaggtcata ccaccgcaac cgcagaataa atcgatgagt ttataacgtt
       61 tttcgctgat catgttgtgc atctcggctt cgcttactta tgtacgacta catcctctat
      121 tggaaaatac gtcactcgaa gaacatagtc acccttcgta cgaaactcgc atccgcaatg
      181 ttttgtgtct atccgtcttc ccacgtgcct cgtgatttct tagtctatta tcatcgtaac
      241 acgtatgacc gctttctatg aggattgaat tcatgaaaaa aagcttatca cccctttcga
      301 ttcatggcgg cgaaccggca gcgaagaatg tggtattccg caaatggccc gctacgacga
      361 aagaagacga agcactcgtg ctcgcgtcac ttcggcagga tagccacgca ttcgggccgc
      421 acgcggatat gctgcagaag gaattcgccg catggaacgg gaatcgtttc tgcctcgcga
      481 cgaacgccgg aacggcggcg ctccatatgt gcatcgccgc gtgcgacgtg cgtcccggcg
      541 atgaggtaat caccaccgcg ctttcgtgga ccagcagtgc gacggcgatc atccatcaca
      601 atggcatacc cgtgttcgtc gacgtggatt gggactccat gtgcatggat ccggcgaaaa
      661 tcgagacggc gatcagcgag aagacgaagg cgatcctcgt cgtgcattac tggggcgttt
      721 cgtgcgacat ggacccgatc atggatatcg ccaaacggca caacctcaag gtgatcgagg
      781 acgcgtgcca ggcacacggt gcgctctaca acgggcgcaa agtcggcacg atgggtcaca
      841 tctccgcgtt cagtctgaat cagaacaaga atctgtgcgg aggcgaaggc ggcctgctcg
      901 ttaccgacga cgagaacctg ctcgaacgcg cgcgcgccgt gtggagcttc ggcgacatgc
      961 gtccggcgga ttccgggcgc gactaccata actacggcct cggatatatg tatcgcacgt
     1021 ccgatctcgc cgcggcattc gcgctttcgg gactgcgacg gttggatgag acgaatcgac
     1081 acgcgcaggc gaactggaag cggctcgatg acgcactcat cggcacaccg aatctcgtgc
     1141 ggagctggtc gacgccggaa cgtccgacga acgggtatgc gtacgttctg cgcgtcgatc
     1201 cggcgtatgc gaaacagcgc ggcgtgacac tgcgcgccat gaccgaggga atcgtggcgg
     1261 cgctcaaggc ggaaggcgtg aacacgtcta tcgcgaattg gctcctgccg gcgcacgcgg
     1321 tgtttcaggc gaaaaacgcg tacggaaacg gcgaaccgtg ggcaagtcat gcgcgaccgg
     1381 aagtgtcgta cgcactggat cagttccccg tggcgcaaca gtgcatcgat acgtgcctgt
     1441 ggggcatcaa caaccaccgt ccgccgaatg gaccggagca gatcgacgcg ctcgctgcgg
     1501 cgattcggaa agtattcacg catcttgacg acgtgccggt cgcctgacga gacgcacgcc
     1561 agtagacgat aggaatggga ctacgcgatt ctctcccgga ggaatgacaa tgcgagtcgg
     1621 aaccgcatac cgcgatatca cacctgatcg cccgcttgcg cttcaggggc aaatgcatat
     1681 ccgcatcgcg aaatacacgc gcgatccgct tacggtaaac gcgatcgtgt tcgaggaagg
     1741 tgggaagcgc gtcgcgctga tttccaccga tgtgtgcgtt ttaccgaatc acctcgccga
     1801 ggagatcaaa cgcgcggtgt cggaagtctc gggaatacca gtacaggaca tcatccccgc
     1861 tgcgacgcac acccacgtcg gtccgtgcac gacgacacgg ctgttcggcg atatcgatcc
     1921 gacatggcgc gaacacctca tcaaatcgac tgccgatgcg gtgaagtccg cgattgacga
     1981 cctcgaggac gccgagttat tcaccggcgc ggggtggctc gatcacatgg gatg
//
LOCUS       DSCC01000334            4087 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1014709, whole genome shotgun sequence.
ACCESSION   DSCC01000334 DSCC01000000
VERSION     DSCC01000334.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1014709"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            226..936
                     /locus_tag="ENN56_04150"
     CDS             226..936
                     /locus_tag="ENN56_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74715.1"
                     /translation="MYHVDADIDEIADYLTRKNATILDSVVTSFFISFTLDTISLRLP
                     NTSVIPQRFDVWMSNDCHPDTLDALRRVWQRATESDDSGNTWTEILSQFVSPIVTRGG
                     LVCWTVNATSRFETTLAIDGLLETRLPDGFAVAVADYRGADHIRTNPELERLEGILPF
                     LVSGKIGSENNERARTLWFARNGTPEAFRTAIHSRRAAAVIAPTDSDAPSYYGPEEVS
                     DTLRSRTNWRWFKTRSGG"
     gene            1057..1133
                     /locus_tag="ENN56_04155"
     tRNA            1057..1133
                     /locus_tag="ENN56_04155"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:1091..1093,aa:His,seq:gtg)
     gene            1258..1656
                     /locus_tag="ENN56_04160"
     CDS             1258..1656
                     /locus_tag="ENN56_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74716.1"
                     /translation="MARRISETLRKTGMNVPVEFSDDSQRVRHYEEREDRSEETEHDR
                     YRRKQARLAEQDTSTSLPIKVVQDEVVKPKAGTRFEGPIEKLIETYKDPREVVRHMTM
                     GQYMRYELRGGQIWEGDSLIYDGTAHLQET"
     gene            1788..2600
                     /locus_tag="ENN56_04165"
     CDS             1788..2600
                     /locus_tag="ENN56_04165"
                     /inference="COORDINATES: protein motif:HMM:PF02151.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74717.1"
                     /translation="MNEEAPKNNDISPILEGWDYRPDRPAVRQIVGNDGKPKIQLRLD
                     LGLLQMEATGRPDGKRPHGFDTLLAHYRAMLESDHVVSEGENDFTLNEEECVKLQLES
                     MQFYHRRISWLELGEYSAAEADAQHNLDIITFVTEHAQTDQLKEMFVQWKPFVLVHRT
                     MARARKAWEKSEFDRAIEIIEEGVGDIAQAYRDQGREDAVENAGEIAFLRKWAEEIDQ
                     TRPLSLRQRLERELDSAVADEAFERAAVIRDRLRDMGAEGDEISTSDPPTSE"
     gene            2678..2753
                     /locus_tag="ENN56_04170"
     tRNA            2678..2753
                     /locus_tag="ENN56_04170"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:2711..2713,aa:Phe,seq:gaa)
     gene            2914..>4087
                     /locus_tag="ENN56_04175"
     CDS             2914..>4087
                     /locus_tag="ENN56_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74718.1"
                     /translation="MGNTGNTRHGATFYVSKLGDNSDGRSWSTAFRSLQAALGAIPDA
                     RGGHRIIVRPDTYLEGNLHPAHAGAPDAYNTLDVDWDGSLGSGASGYAVVDASEAERG
                     MQSVDWWGFPRCTPEHSGVDWDRWVLRHIYATGGDGGLFWDLPFKIAPFSIVVEDSVG
                     IGRAFGGGVGHVLPRANEPIVFRRCTLWSLDWWGDTAGAYVRAENPSPPEHPDVFFES
                     CTLVGPQCALKSGNPGFSSYSRVSLNNCILVALNFSQPHGTPTDGIIQSVIDGKYLHV
                     DLHDTTLMGYKVFGVREHPETESDIGYTANGSIRAYVQYQQSVPKGMQPLGHWPIDVF
                     AKIQPPSLTPPQDTQFRRPLFGSFELVQRDMCELTPIIWHGRLCHLMSVRPGSGGDM"
BASE COUNT          932 a         1114 c         1139 g          902 t
ORIGIN      
        1 atccccgacg catcgtcgcg tctcacatcg accgctgacg cgcttcgagc gctcggcgcc
       61 atcgttgaag ctccgtcgtc ggtaattgat gtaaaatctc gtaccagcgt cttaccgaag
      121 accgtgaaat cgtggaaatc ggattcggta tttgccgttt attccggtcc acgttctcgt
      181 acaccctata cgacgcgcgc tcgtctttgg gcctcgggaa tccctatgta tcacgtcgac
      241 gcggatatcg acgaaatcgc ggattacctg acgcgaaaga acgccacgat cctcgattcc
      301 gtcgtgacgt ctttttttat ttcgttcaca ctcgatacga tctcgctacg tctcccgaat
      361 acctcggtga ttcctcaacg tttcgatgta tggatgtcga acgattgcca cccggatacg
      421 cttgacgctc tgcgtcgagt ttggcaacgt gcgaccgaat cggatgatag cgggaatacg
      481 tggacagaaa tcctcagtca attcgtctcg ccgattgtga cgcgtggtgg actggtttgt
      541 tggacggtga atgcaacatc gcgttttgag acgacactcg caatcgacgg tctcttagaa
      601 acccggcttc cagacgggtt tgccgttgcg gtggcggatt atcgtggagc ggatcacatc
      661 cggacgaatc ccgagttgga acggttggag ggcatcctac cgtttctcgt ttccggcaag
      721 ataggatccg agaataacga acgcgctcga acactttggt tcgcacgcaa cggcactccc
      781 gaagcgtttc gcacggcgat tcactctcga cgcgcggctg cagttatcgc gccgacggat
      841 agtgacgcgc cttcgtacta cggacctgag gaagtaagcg acacgcttcg ctctcggacg
      901 aactggcgtt ggtttaaaac gcgcagcggc ggttgacgaa tggactcaca cgccgtacat
      961 taaactgact atattggttt gctcgacgcg ctcgaaacgg cgagcgtttc gtgtcgcgtt
     1021 gatcgtgcac gtataaacgc gcgacggatt tgtgcggtga gtgtagctca gctggttaga
     1081 gcatcagact gtggctctga gggccggggg ttcgaatccc ctcactcacc ccatcgtata
     1141 cgcaaagatg cctcggatac ggacgtattc gaggcatctt catttgtacc cgtatcggaa
     1201 cgggcgtaat agtgttttca tttgttgatg aatcgcaact cgtaagagag gactcatatg
     1261 gcacgacgta tctctgagac gcttcgtaaa accggtatga acgtacccgt ggagttttcg
     1321 gatgactccc agcgcgttcg ccattacgaa gaacgagaag atcgtagcga ggaaactgaa
     1381 cacgatcgtt accggcgcaa acaagctcga ctcgccgaac aggacacgtc gacaagtctt
     1441 cccatcaaag tcgttcagga tgaggtcgtc aagcccaagg cgggaacccg gtttgaaggt
     1501 ccgatcgaaa agctgatcga gacgtacaaa gaccctcggg aagtcgtgcg acacatgacg
     1561 atgggccagt acatgcgcta cgagctacgc ggtggtcaga tttgggaagg cgattccctg
     1621 atctatgatg gaaccgctca ccttcaagag acataacagc gagtcggagt gaaccgaacc
     1681 gtctcgaacg gcgttatatt tcctaacgtg ccgttattcg ttatacccgc gtacgcgttg
     1741 cgaatcgacg acgcgtacgc gtttttgttg agatatgaga taagttcatg aacgaagaag
     1801 caccgaaaaa caacgacata tcaccaatcc ttgaagggtg ggactaccgc ccggaccgtc
     1861 cggcagtgcg tcagatcgtc ggcaacgatg gaaaaccgaa gattcaatta cgtctcgacc
     1921 tcggcctcct gcaaatggaa gcgaccggtc gaccagacgg gaagcgccct catggcttcg
     1981 atacgttgct cgcccactac cgcgcaatgc tggaatcgga tcacgtcgtt tcagaaggcg
     2041 agaacgattt cacgttgaac gaggaggagt gtgtaaagct ccagcttgag agtatgcagt
     2101 tttaccatcg acggatcagt tggcttgaac ttggagagta ctcggccgcc gaagcggacg
     2161 cgcaacataa tcttgatatc atcacattcg tgaccgaaca cgcgcaaacc gatcaactca
     2221 aagaaatgtt tgttcaatgg aagccctttg tcctcgtaca ccgcacaatg gctcgggctc
     2281 gtaaggcgtg ggagaaaagt gagttcgacc gtgccatcga gatcattgag gagggagtag
     2341 gcgatattgc gcaagcgtac cgcgatcagg gtcgtgaaga cgctgtcgaa aacgccggcg
     2401 aaatcgcctt tctgcgaaaa tgggctgaag agatcgacca aacccgaccg ctatcgctac
     2461 ggcaaagact cgagcgcgaa ctcgactcag cggtcgccga cgaagcgttt gaacgtgcgg
     2521 ccgtgattcg agaccgcctg cgcgatatgg gcgccgaagg ggatgaaatt tcgacgagcg
     2581 atcctccgac atcggagtaa gcgttctccg tcgacgtttt ttcacacgtc ggtcttgact
     2641 gagagaacga agcttcctac tcttttaagt cttcggtggc gctctagctc agttggtaga
     2701 gcaagggact gaaaatccct gtgtccccag ttcgattctg gggggcgcca ccatgaactt
     2761 gcacgaaaaa cgcttcgagc aacaaagctc gaagcgtttt tcgcgtacgc tccctattgg
     2821 cgttacgtat tcgacttccc cccacgcgac ttctcatcga tctttcccta ttatccaacc
     2881 accgattatc caaccaccga agacgaggat tctatgggga atacgggaaa cacgcgtcac
     2941 ggcgcaacgt tctacgtgtc gaaactcgga gacaattccg acggtcgttc gtggagcacg
     3001 gcgttccggt cgctccaagc ggcactcggc gcgattcctg acgcgcgtgg cggacaccgt
     3061 atcatcgtgc gtcccgacac gtatctcgaa gggaatctcc acccggcaca tgcgggtgcc
     3121 cctgacgcgt ataacaccct cgacgtcgat tgggatggca gccttggatc gggagcgtcg
     3181 gggtacgcag tagtcgatgc aagcgaggcg gaacgcggaa tgcaaagcgt tgattggtgg
     3241 ggattcccac ggtgcacgcc ggagcattcg ggagtcgact gggaccgttg ggtactccgc
     3301 catatttacg caactggcgg tgacggcggg ctcttctggg atctaccgtt caagattgcg
     3361 ccgttttcta tcgtcgtcga ggacagtgtc ggaatcggac gagcgtttgg cggcggagta
     3421 ggtcacgtgc ttccgcgcgc gaatgagcca atcgttttcc gtcggtgcac cctctggtcg
     3481 ctcgactggt ggggcgatac ggcgggagca tacgtacgcg cggaaaatcc ctcgccgccc
     3541 gaacaccccg acgtcttttt cgagtcctgt acactcgtcg gtccgcagtg tgcgttgaag
     3601 tcgggcaatc cgggattttc ttcgtattct cgtgtttctc tcaacaactg catcctcgtc
     3661 gctctcaact tttctcaacc gcatgggact ccgaccgacg ggataatcca gagtgtcatc
     3721 gacgggaagt atctccacgt cgacctgcat gataccacgc ttatgggata taaagtcttc
     3781 ggggttcgag aacatcccga gacggaaagt gatatcgggt atacggcaaa cggaagcatc
     3841 cgcgcgtacg tccagtatca acagagcgtt ccgaaaggta tgcaaccact cggacactgg
     3901 ccgatcgatg tcttcgcgaa aatccaacca ccgtcactga caccaccgca agacacacaa
     3961 tttcgacgcc cactatttgg atcgtttgag ctcgtacaac gagacatgtg cgagttgaca
     4021 ccgatcattt ggcacggacg cctctgccac ctcatgagtg ttcgtccggg atcgggtggt
     4081 gatatgc
//
LOCUS       DSCC01000335            2460 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029119, whole genome shotgun sequence.
ACCESSION   DSCC01000335 DSCC01000000
VERSION     DSCC01000335.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029119"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            125..346
                     /locus_tag="ENN56_04180"
     CDS             125..346
                     /locus_tag="ENN56_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDS74719.1"
                     /translation="MKTTTLTIRLEPELDRLLTKASEQTGISRSELAREALRRQLRVA
                     RFDQLRKRIMPFAEARGYLMDEDVLGDVS"
     gene            343..744
                     /locus_tag="ENN56_04185"
     CDS             343..744
                     /locus_tag="ENN56_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDS74720.1"
                     /translation="MRVFLDTNVLVSAFSTRGLCADILREVLGAHELIVSEQVLSELR
                     RILVEKFGVPNEIADEIDGFLREDSVFGDPEPLIDLVFDDKDDVPILSAAVHGNTKLF
                     VTGDKAVHALGRIGAMRIYLPREFWNELRNA"
     gene            856..1140
                     /locus_tag="ENN56_04190"
     CDS             856..1140
                     /locus_tag="ENN56_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74721.1"
                     /translation="MLVDLGCAVISDVDAKVTAAVVGESLSFEDRDVRGVRVRGQDGV
                     DYTVVANFGDTNLMASIDSRDTVVPAGSAIVAAADFAYTSQVPTRSESDT"
     gene            1156..>2460
                     /locus_tag="ENN56_04195"
     CDS             1156..>2460
                     /locus_tag="ENN56_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HDS74722.1"
                     /translation="MDSILAHNVASLGPFVPELILIATLLAVILADVVTKGDRIALTA
                     GIGVGGTTLSLLAVLYSLPYERTSLFHGMIAHDMFAVFFKLIAAISVIIVLIVSVQSV
                     DVRRGTFAEYVAMMVAMALGMYVLASAVNLLSMFLAFELVSMTSYVLTAHQKDQLPSS
                     EAALKYVIYSAASAATMIYGMSILFGLTGSLAASDIAVALVGGESSLAIAVAVLFILG
                     GIGYKIASVPFHFWCPDAYQGAPAPVAALLSVGPKAAGMAFLARFFYEAMAIPEGLAW
                     QPVGLADWQIVIAIISAVTMTLGNLSAIWQRSMKRLLAYSSIAHAGYILMGVAMISQM
                     GLRAMMFYLAVYLFMNLGAFVVIIALADKFESDDIDSYAGLGGRAPFVAVSMAVFLIA
                     LAGIPPTSGFIGKFYLFWAVLEANNLIWLAVIAALNTVVSLYY"
BASE COUNT          480 a          625 c          765 g          590 t
ORIGIN      
        1 gaagacgcga aggagtggac agagtcacac agatgcgctt ggaacgacac cgtgcgacgc
       61 tcttttgttg agcaactcct acgttttctt gacgagttcg cgcgcttgtg ttacaatgta
      121 atacatgaag acgacgacac tgacaatacg actcgagccg gagctggatc gcttgttgac
      181 gaaagcgagc gagcagacgg ggatttcccg cagcgaactc gcgcgtgagg cgcttcgacg
      241 tcagcttcgt gttgcacggt ttgatcagct ccgtaagcgg atcatgccgt ttgccgaggc
      301 acgtgggtac ctcatggatg aggacgttct cggcgacgtg tcatgagggt gtttctcgat
      361 acaaacgtat tggtgagcgc attctcgaca cgcgggctct gcgcggacat tctcagagag
      421 gtcctcggag cgcacgaact catcgtatcg gagcaggttc tctccgagct tcgacgaata
      481 cttgtcgaaa agtttggtgt cccgaacgaa atcgcggacg agatcgacgg ctttcttcga
      541 gaagacagcg tgttcggcga tccagagccg ttgatcgacc tcgtattcga cgataaagac
      601 gacgtaccga tactttctgc tgcggtacat gggaacacca agttgtttgt cactggcgac
      661 aaggccgtgc acgcgctcgg tagaataggt gcgatgcgca tatatctacc gcgtgagttc
      721 tggaacgagc tgcgaaatgc gtgacgttcg atttgggaac gtctcacgcg aagacgcgaa
      781 gggataacgt acacttggta cgagcatcga ttgatataac ggcagtcatc tcagcgctcg
      841 aacgctgggc atcaagtgct cgtcgatctc ggttgcgcag tgatttcgga tgtcgacgcg
      901 aaggtgacgg cggcggtcgt tggcgaatca ctcagttttg aagatcgcga cgtgcgcggg
      961 gtgcgtgtgc gcgggcagga cggcgtcgat tacactgtcg tagcgaattt cggtgacacg
     1021 aaccttatgg catcgataga tagcagggat accgtagttc cggcaggatc cgcgattgtt
     1081 gcggctgcag atttcgcata tacgtcgcaa gtgccgactc gaagtgaaag tgatacttga
     1141 tccgtaggat cggatatgga ctcgatactc gcacataacg tagccagcct cggcccgttc
     1201 gtgccggagc tcattctcat tgcgacgttg ctcgccgtga tacttgccga tgtcgtgacg
     1261 aagggcgacc gaatcgcgct cacagcggga atcggtgtcg gcggtacgac gctgagtctg
     1321 ctcgcggtgc tgtattcgtt accgtacgaa cggacgtcgt tattccatgg gatgatagca
     1381 cacgacatgt tcgccgtgtt tttcaagctc atcgcggcga tatcggtcat catcgtgctg
     1441 atcgtatcgg tgcaatcggt cgatgttcgg cgcggcacct ttgcggaata cgtcgcgatg
     1501 atggttgcga tggcgctcgg gatgtatgtg ctggcgtctg cggtcaattt gctgtcgatg
     1561 tttcttgcgt tcgaactggt cagtatgacg tcgtacgtgc ttaccgcgca ccagaaggac
     1621 caactgccgt cgagtgaggc ggcgctcaag tacgtgatct acagtgcagc atcggccgcg
     1681 acgatgattt acggtatgtc gattctgttc ggactgaccg gatcgcttgc ggcgagtgat
     1741 atcgcggtgg cgctcgtcgg tggcgaaagc tcactcgcaa ttgccgttgc ggtgttgttc
     1801 atccttggcg ggatcggata caagatcgcg tcggtaccgt tccatttctg gtgtccggac
     1861 gcgtatcaag gggcacccgc gccagtagcc gcactactca gcgtaggccc gaaggccgcc
     1921 ggtatggcat tcttggcacg gtttttctac gaggcgatgg cgattccgga aggcctcgcg
     1981 tggcaaccgg tcggactcgc cgattggcag atcgtgatcg cgatcattag tgcggtaacc
     2041 atgacgctgg ggaatctctc ggcgatctgg cagcggagca tgaagcgact gctcgcgtat
     2101 tcgtcgatcg cgcacgctgg atacatcctc atgggcgtcg cgatgatctc gcagatggga
     2161 ctccgcgcga tgatgtttta cctcgcggtg tacctgttca tgaatcttgg cgccttcgtt
     2221 gtgatcatcg cgctcgcgga caagttcgag tccgacgata ttgattcgta tgcaggcctc
     2281 ggaggacgtg caccgtttgt cgcggtgtcc atggcggtgt ttctcattgc gctcgccggt
     2341 atcccgccca cgtctgggtt tattggcaag ttctacctgt tctgggcggt gttggaagca
     2401 aacaacttga tctggctcgc ggtgatcgcg gcattgaata cggttgtgtc gctgtattac
//
LOCUS       DSCC01000336            2677 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025986, whole genome shotgun sequence.
ACCESSION   DSCC01000336 DSCC01000000
VERSION     DSCC01000336.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025986"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..625
                     /locus_tag="ENN56_04200"
     CDS             <1..625
                     /locus_tag="ENN56_04200"
                     /inference="COORDINATES: protein motif:HMM:PF13561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS74723.1"
                     /translation="GDISQESVNKEMIERTVDKFGRIDTIVTCVFWSKSGNSQQITLE
                     EWNQCLAVTLTSTFLAAKYGVPAIRETAGKGNIIAISSVHGSDPGRNYAMYATAKAGL
                     NMLVRALAYDHTSEGVRVNAVAPGATYADRRMVDPNDTTGVGSLYLTGHYVSGDDIGD
                     VVAFLASDRAQSITGQLITVDGGASIPEYNWLMHENEQKWKKKLEAK"
     gene            complement(747..2006)
                     /locus_tag="ENN56_04205"
     CDS             complement(747..2006)
                     /locus_tag="ENN56_04205"
                     /inference="COORDINATES: protein motif:HMM:PF13378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HDS74724.1"
                     /translation="MPAKVTNVERFTCIVPMVERIRREMERADIHNWAVTEICRVETD
                     AGVVGWGETIIHYTWGRVTDDAVKRVIGHPPTEFMWDDSLGAGLQMALFDAAGKIAGV
                     PIHRLLGKQTRTLCPASFWDHDMGPEAYEREAKVASDLGYTSMKIKTRPWHDVYETMR
                     RISEATPEWFKIDCDWNDHLSDVSTAVPVLRELECDFPKISIWESPMRMGDASGNRLL
                     RDRIDRAIAHHYGAVPIRTAIELGVCDGFVIGGGANRIMSDGNLSAQVHMPFFLQMVG
                     TGLTTTMALHLGAVLTHAEWPMVTCHELYEHNLLTQRLDVVGGYIRVPEGPGLGVEVD
                     EAALAKYRTKETDIPLPRQLVCITRPMGVKIWSARSMYSVWPFYKEGNAPRYERGVYT
                     ELIDDDGSKHFDDLWKRSQKWPVVTES"
     gene            complement(2019..2402)
                     /locus_tag="ENN56_04210"
     CDS             complement(2019..2402)
                     /locus_tag="ENN56_04210"
                     /inference="COORDINATES: protein motif:HMM:PF05168.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HDS74725.1"
                     /translation="MTDDVQKHLTRADLSLRAAERMMPEFPSDAISRAYYAVFHAAKA
                     VALLLDIDRSSHHALWSVFGREVANTELMPRECHKYALDLFSARMSSDYLADPADTVA
                     LAERHLQNAREFVAAAKTFIEEHTR"
     gene            complement(2399..>2677)
                     /locus_tag="ENN56_04215"
     CDS             complement(2399..>2677)
                     /locus_tag="ENN56_04215"
                     /inference="COORDINATES: protein motif:HMM:PF01909.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDS74726.1"
                     /translation="EIERIYGDRLRAVYLYGSYARGDARDDSDIDIAVVLDGPVDQNE
                     EIFRVSDVRAEMSLSEDCLIMPLFVSYTDLIDSRESIHKNIAGEGVAL"
BASE COUNT          556 a          767 c          791 g          563 t
ORIGIN      
        1 cggagacatt tcgcaggaat ccgtcaataa ggagatgatc gagcgtaccg tcgacaaatt
       61 cggtcgcatc gacacaatcg ttacctgtgt gttctggtcg aagagcggga attcgcagca
      121 gatcaccctc gaggagtgga atcaatgtct cgcggtgacg ttgacgtcga cgtttctcgc
      181 ggcgaagtac ggtgtcccgg cgattcgcga gactgcgggg aaagggaaca tcatcgccat
      241 atcatcggta cacgggtcgg atcccgggcg aaactacgcg atgtacgcga cggccaaggc
      301 gggcttgaat atgctcgtgc gcgcgctcgc gtacgatcac acgtcggaag gggtacgggt
      361 gaacgccgtt gcgccgggcg cgacgtacgc ggatcgccga atggttgatc cgaacgacac
      421 gaccggcgtc ggttcgctgt atctgacggg gcactatgtc agcggcgacg atatcggaga
      481 cgtcgttgcg tttctcgcat cggatcgagc gcaatcaatc accgggcaac tcatcacagt
      541 cgatggtggc gcgtcaatcc ccgagtacaa ctggctcatg cacgagaacg aacagaaatg
      601 gaagaagaag ctcgaagcga agtgatgcgt gggtacttcg tagcaaacac tgtctcaaca
      661 cgagcaattc tgttgttgag cgtactttaa aaacgaaggc cgtcattttg agaatgacgg
      721 ccttcgtttc atgatgacgg aggacttcag ctctccgtca ccaccggcca tttctgggag
      781 cgcttccaga ggtcgtcgaa gtgcttcgag ccatcgtcgt cgatgagctc cgtgtagact
      841 ccgcgctcgt accggggcgc gttgccctct ttgtagaacg gccaaacgct gtacatgctg
      901 cgagccgacc agatcttcac acccatgggt cgcgtgatgc agacgagttg gcgtggcagc
      961 gggatgtcgg tctctttggt gcggtatttc gcaagcgccg cctcatccac ttccacgccg
     1021 agaccgggtc cttccggcac gcgaatgtac ccgccgacca cgtcgagccg ttgtgtgaga
     1081 aggttgtgct cgtagagttc gtggcaggta accattggcc attcggcgtg tgtgagcacc
     1141 gcgccgaggt gaagcgccat ggtggtggtg agtcccgtac cgaccatctg aaggaagaac
     1201 ggcatgtgca cctgcgcgct gagattgccg tcggacatga tccgattcgc gccaccgcca
     1261 ataacgaacc catcgcagac accgagttcg atcgccgtcc gaatcggcac cgcgccgtag
     1321 tggtgcgcga tcgctcggtc aatccgatcg cggagcagtc ggttcccgct cgcgtcgccc
     1381 atgcgcatgg gactttccca gatgctgatc ttcgggaagt cgcactcgag ttcacggagc
     1441 accggcaccg ctgtcgaaac gtcggaaagg tgatcgttcc agtcgcagtc gatcttgaac
     1501 cactccggcg tcgcctctga aatccgccgc atcgtctcgt agacgtcgtg ccacggccgc
     1561 gtcttgatct tcatcgacgt gtagccgaga tccgacgcga cttttgcctc gcgctcgtat
     1621 gcttccggcc ccatgtcgtg atcccaaaac gacgcggggc aaagcgtgcg cgtctgcttg
     1681 ccgagcagcc gatgaatcgg aacgcccgcg atcttacccg ctgcgtcgaa caatgccatc
     1741 tggagaccgg cgccgagtga atcgtcccac atgaactcag tcggcggatg tccgatgacg
     1801 cgcttcaccg catcgtcggt aacgcgcccc cacgtgtaat ggatgatcgt ctcgccccat
     1861 ccgacgacgc cggcatcagt ctcgacgcgg cagatctcgg tgacggccca gttgtggata
     1921 tcggcgcgct ccatttcgcg acggatgcgc tcgaccatgg gaacgataca cgtgaagcgt
     1981 tcgacgttgg taaccttcgc gggcatggtg gattcctctt agcgggtgtg ttcttcgatg
     2041 aatgtcttcg cggctgcgac aaactcgcgt gcgttttgaa ggtgccgttc ggccaatgcg
     2101 actgtatcgg cggggtctgc aagatagtcg ctcgacatgc gagctgaaaa gaggtcaaga
     2161 gcgtatttgt gacattcgcg tggcatcagt tccgtattcg caacttccct cccgaaaact
     2221 gaccagagtg cgtgatgaga cgatcgatca atatcgagta acagcgcaac cgcttttgcc
     2281 gcatggaata ccgcgtaata cgctcgacta attgcgtctg aggggaattc aggcatcata
     2341 cgttctgccg cgcgtaaaga caaatcggca cgcgtaaggt gtttttggac gtcgtcggtc
     2401 acaacgcaac accctcacca gcgatatttt tgtggatact ctcacgagag tcgataaggt
     2461 cagtgtatga gacgaataac ggcataataa ggcagtcttc gctgagcgac atttctgccc
     2521 gtacgtcgct cacgcggaat atttcttcgt tctggtccac cggcccgtcc aacacgacag
     2581 ctatatcgat atcggagtcg tcgcgagcat cgccgcgggc gtacgatccg tagaggtaga
     2641 cagccctgag tcgatcgccg taaatccgct cgatctc
//
LOCUS       DSCC01000337            1635 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1050652, whole genome shotgun sequence.
ACCESSION   DSCC01000337 DSCC01000000
VERSION     DSCC01000337.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1050652"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..272)
                     /locus_tag="ENN56_04220"
     CDS             complement(<1..272)
                     /locus_tag="ENN56_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74727.1"
                     /translation="MHNGMRRDFAYTVFLILVAGFMGTSLMPDSVSADPDGDFAFAEH
                     LMRDEMYDLAAQQLQLFVNNHPEHPKTADAFLLLASAYTERNEYARA"
     gene            complement(438..1541)
                     /locus_tag="ENN56_04225"
     CDS             complement(438..1541)
                     /locus_tag="ENN56_04225"
                     /inference="COORDINATES: protein motif:HMM:PF13860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74728.1"
                     /translation="MEMTATFMEEVMYDNVNDYHQYLDPIGWGSAGISIFSEPGNGLY
                     FNNGTIYPYGGAIFPIFLTEYYGSDAAFDIIHDTFHVRKVTSTTRIVESIQARIGLPI
                     EEILAEFWVWNYFTGYRARDDFGFPEAASYRPAPLDPDAYAEAATAHTIESLADSGVV
                     SGLVGAAPLGAALIRIVPDGSLGGFRATLYDSTEGNWSWRIAVADENGVRRFPETPTL
                     YDDRTRTITIAGDNWTNATDIVLVGTNGVTSGGEQLFTFELKYDATLTSIAVREALPD
                     RVSLGLNAPNPFNPSTTIPFSLPVPGHTELAVYAATGQLVRTLIDTDMRSGEHAVIWD
                     GRDNSGRPAASGVYLVRLASDAGHDVRRISLIR"
BASE COUNT          381 a          452 c          459 g          343 t
ORIGIN      
        1 gcgcgagcgt actcgttgcg ctcggtgtac gccgatgcca acagaagaaa cgcgtccgct
       61 gttttcggat gctctggatg gttgttgaca aaaagctgga gctgttgcgc ggcaaggtca
      121 tacatctcgt cgcgcatcag gtgttccgca aacgcgaaat caccgtcggg atcggcagat
      181 accgagtccg gcatcaggga cgttcccatg aatcccgcga caagaatcag aaaaaccgtg
      241 tatgcgaaat cgcgccgcat gccgttgtgc atgcatcctc ctgcggaaat cgggtgtttt
      301 acgtctttgc gaggaaattc atggacgcgc cgttcacatc cgttgttccc gacgcgatgg
      361 aaagccaatg tggcagaacg gaatgtacga aacggcagtc gcctccgtca attcaaccgt
      421 cttttcggac gagggactta gcgaatgagt gatatccggc gaacgtcgtg accggcgtcg
      481 gatgcaagtc ggacgagata cacgccggat gccgccggtc gccccgaatt gtcgcgccca
      541 tcccagataa cagcgtgctc accggagcgc atatccgtat cgatgagtgt gcggacaagc
      601 tgtcccgtgg cagcatagac tgcgagttca gtatgccccg gaactggaag cgaaaacggt
      661 atcgtcgtcg acggattgaa cggattcggc gcgtttaacc ccagtgagac gcgatcgggc
      721 aacgcctcgc gaacggcgat actcgtaagt gtcgcgtcgt atttcagctc aaatgtaaaa
      781 agctgttcgc caccgctcgt gacgccattc gtaccgacaa ggacgatatc ggtcgcgttc
      841 gtccaattgt cacccgcaat cgtgatagtc ctcgtgcgat cgtcatacag cgtgggcgtc
      901 tcgggaaatc tgcgcacacc gttttcgtca gcgacagcga tacgccacga ccagttaccc
      961 tccgtgctgt cgtaaagcgt cgcgcggaat ccaccgagtg aaccgtcggg aacgatgcga
     1021 atgagcgcgg caccaagcgg ggcagctcca acgaggccac tgacaacgcc cgagtcggcc
     1081 aaactctcaa tcgtgtgcgc cgttgctgcc tctgcatacg cgtccggatc aagcggagcg
     1141 ggacgatatg aagcggcttc ggggaatccg aagtcatcgc gtgcacggta tccggtgaaa
     1201 tagttccaca cccagaactc agcgaggatt tcctcaatgg gcaaaccaat tcgtgcttga
     1261 atagactcga cgatacgcgt cgttgaagtc actttccgaa cgtggaatgt gtcgtgaata
     1321 atgtcgaagg ctgcatctga gccataatat tctgtgagga aaatcggaaa aatcgcaccg
     1381 ccatacgggt agattgtacc gttgttgaag tacaagccgt tccccggttc actaaaaatc
     1441 gaaatcccgg cggagcccca ccctatcggg tcgaggtact ggtggtagtc gttcacgttg
     1501 tcatacatga cctcttccat gaatgtcgcc gtcatctcca tccaccaact tattcctgac
     1561 gtgtacgaga catattgaaa ctgcacggca tggaagagtt catgtgcaat ggttactcgg
     1621 agtgcagcgt tgccc
//
LOCUS       DSCC01000338            3241 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020155, whole genome shotgun sequence.
ACCESSION   DSCC01000338 DSCC01000000
VERSION     DSCC01000338.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020155"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1040
                     /gene="lysA"
                     /locus_tag="ENN56_04230"
     CDS             <1..1040
                     /gene="lysA"
                     /locus_tag="ENN56_04230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDS74729.1"
                     /translation="IVSGGELYRARTAGFPANKIVFSGVGKTADELRAALCEDILAFN
                     VESLGELEALNRVAANERRKARVNLRINPDVDPKTHPKIATGLKTAKFGIPHAKARDA
                     YRRAAELEWIEVVGIDAHIGSGLTSVKPFVEAAERLLGLVEQIRADGVSLSMIDIGGG
                     LGITYDAENPPSPAERAAAVGPILSDAGLTIILEPGRSVVGNAGVLLTRVLYLKENEG
                     KNFVVVDAGMNDLIRPSLYDSFHRVLPVIERDRQEIRADIVGPICETGDVLARDREIV
                     EPYESDVLAVMSTGAYGFAMASTYNSHPRPAEVIVRDAEWQVIRDRESYDDLIRGERF
                     LTIGEKASSDA"
     gene            940..2094
                     /gene="thiL"
                     /locus_tag="ENN56_04235"
     CDS             940..2094
                     /gene="thiL"
                     /locus_tag="ENN56_04235"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDS74730.1"
                     /translation="MPNGRSFETGNRTMILFAASGFSRSVKKHLRTRNAMNLSDLGEF
                     GFIDRISGILNTADVRVVKGIGDDCAVVRLSDDRVQVITTDALVENVHFLTDRIGAEQ
                     LGHKCLAVNLSDIASMGAEPTFAVVTVASPANGDVDYLAGVFRGIRALADRFGVTIVG
                     GDMTSSQSDLMISLTALGEARVDEIRYRSDAHPGDALLVAGVIGESGAGLATLLDDEC
                     AGRLRRETCDTLRSRHLTPEPLVREGCWLARYAGPAGEKVGAMLDVSDGIASDVRHIC
                     RESSTRCNTPVGVRINEAELPQTAALAEYLECVGGDSIRYLLQSGEDYALLFSAPAST
                     TDDLMERFHAEFATPIARIGTFTDDERVVLYRRDGSESSLSGGHDHFRHA"
     gene            2187..>3241
                     /locus_tag="ENN56_04240"
     CDS             2187..>3241
                     /locus_tag="ENN56_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74731.1"
                     /translation="MLWLRGIVMPVRIGFISFAHGHTVTYADQLIHMKEVELVACWDD
                     DADRGRETANRYTMRFVPNLDDLLGDGSIDTVIIGSPTNRHADHAVAAAKAGKNILLQ
                     KPMALSLTDCDRIIEAVNAADIVFSMAYQMRCDPVNRWMRTHIRDGNLGKLGIARRRH
                     CIPVLFNEGFVTGKTRWHIDPAQNMGMWMDDASHAMDWFFWMFGRPTSVIAEVDNVLT
                     DVAPDDTGAAIFRFPSSEWIAEGMFGLLLNSSVVHAGMNTTELYGDEGVLTQNFGDSP
                     ATSMEWPDDAKPVHWMRADQGDRVWQTPDLPLQRQGERIAQVPRPFIDAVAHGAPVIA
                     TAEEGRVAVEMVLAAYES"
BASE COUNT          640 a          907 c         1028 g          666 t
ORIGIN      
        1 acattgtatc gggcggggaa ctgtaccgcg cgcgcacagc ggggtttccg gcgaacaaga
       61 ttgtcttcag cggcgtcgga aaaaccgccg atgagcttcg cgcggcatta tgtgaagaca
      121 ttcttgcgtt caacgtcgaa tcactcggtg aactcgaggc gttgaatcgc gtcgctgcga
      181 acgagcggcg gaaggcgagg gtgaatcttc ggatcaaccc ggatgtcgat ccgaagacgc
      241 atccgaaaat cgcgacgggt ctcaagacgg cgaagttcgg tattcctcac gcgaaagcac
      301 gcgatgcata ccgccgtgcg gcggagctgg aatggatcga ggtcgtcggc atcgacgcgc
      361 acatcgggtc cggcctcacc agcgttaagc cattcgtcga agccgccgag cgtctgctcg
      421 gactcgtcga gcagattcgc gccgatggcg tttcgttgag catgatcgat atcggcggcg
      481 gattgggaat cacgtacgac gccgagaacc caccaagccc agccgaacgg gcggccgctg
      541 tcgggccgat tctttcggac gccggactga cgattatctt ggaaccggga cgttcggttg
      601 ttggaaatgc aggggtattg cttacccggg tgctctacct caaagagaac gagggtaaga
      661 acttcgtcgt cgtggatgcg ggtatgaatg atttgattcg cccgagcctc tacgattcat
      721 tccatcgtgt tcttccggta atagagcgtg accggcaaga gattcgcgcg gatatcgtcg
      781 gaccgatttg cgagacggga gacgttctcg cgcgcgaccg cgagatcgtc gagccgtacg
      841 aaagtgacgt cctcgcggtc atgagtaccg gtgcgtacgg cttcgcgatg gcgtccacgt
      901 acaactcgca cccgcgaccg gctgaagtca tcgtccgcga tgccgaatgg caggtcattc
      961 gagaccggga atcgtacgat gatcttattc gcggcgagcg gtttctcacg atcggtgaaa
     1021 aagcatcttc ggacgcgtaa cgctatgaac ctgagcgacc tcggcgaatt cggcttcatc
     1081 gaccgtatca gtggcatcct caacaccgct gacgtgcgcg tcgtcaaagg tatcggcgac
     1141 gactgtgcgg tcgttcgact ctcggatgac cgtgtgcagg tcattacgac cgatgcgctc
     1201 gtcgagaacg tccatttctt gaccgaccga atcggagccg agcagcttgg ccacaaatgc
     1261 cttgccgtga atctgagtga tatcgcgtcg atgggtgccg agccgacatt cgccgtcgtt
     1321 accgtcgcgt ctccggcgaa cggcgatgtg gactaccttg cgggggtatt ccgcggcatt
     1381 cgagcgcttg ccgaccggtt cggcgtgacg attgtcggtg gcgacatgac ttcaagccaa
     1441 agtgacctga tgatcagtct taccgcgctc ggtgaggcgc gtgtcgatga aattcgctat
     1501 cgcagcgatg cacacccggg ggatgcgttg ctcgtcgccg gcgtgattgg cgaatcgggt
     1561 gcggggctcg cgacgttgct cgacgacgaa tgtgcggggc gtttgcgacg ggagacgtgt
     1621 gatacgctac ggagtcggca cctcacgccg gaacctctgg tgcgggaagg atgttggctg
     1681 gcacggtatg cggggccggc cggtgaaaaa gtcggcgcga tgctcgatgt ctcggacgga
     1741 atcgcgtcag acgtccgtca catctgtcgt gaaagctcca cacggtgtaa tactcccgtc
     1801 ggcgtgcgca tcaacgaagc ggagttgccc caaaccgctg cgctcgctga gtatctcgaa
     1861 tgtgtaggtg gcgactcgat tcgctaccta ctccaatcgg gcgaagacta cgcgttgtta
     1921 ttcagcgcgc ccgcatcgac caccgatgac ctcatggaac gctttcacgc ggaatttgct
     1981 acgccgatcg cccgtatcgg tacgttcacc gatgacgagc gtgtggtcct gtaccgccgc
     2041 gacgggtccg agtcttcgct ctccggcggt cacgatcact ttcggcacgc gtaagcccct
     2101 tcccccgtcg gtcaccaacg actttccttc caatgtccgc ggacgcagca ctgcggctta
     2161 ttcgacctgc cgatgtttca ttggatatgc tttggttgag aggaatcgtc atgcctgttc
     2221 gaatcgggtt catcagtttc gctcacgggc acaccgtgac gtatgcggac caactcattc
     2281 acatgaagga agtggagctc gtcgcgtgct gggatgacga tgcggaccga ggacgggaga
     2341 ccgccaaccg ctataccatg cggttcgtgc cgaatctgga cgatctactc ggcgacggat
     2401 cgatcgatac ggtgattatc ggtagcccga cgaatcgaca cgccgaccac gccgtcgctg
     2461 cggcaaaagc cgggaagaac atcctactgc agaagccgat ggcgctctcg cttacagact
     2521 gcgaccgaat catcgaagcg gtcaatgcgg cggatatcgt gtttagcatg gcgtatcaga
     2581 tgcgctgcga tccggtcaat cgatggatgc gcactcatat tcgtgatggc aacctcggaa
     2641 aattgggtat cgcgcggcgg cgtcattgca ttccggtgct gttcaacgag gggttcgtca
     2701 cgggaaagac gcgatggcac atcgatcccg cgcagaatat gggcatgtgg atggacgatg
     2761 catcgcacgc gatggactgg ttcttctgga tgttcggccg cccgacgagc gtgatcgcgg
     2821 aggtcgataa cgtgctgacc gacgtcgccc ccgacgacac tggcgcggcg attttccgtt
     2881 ttccatcgag cgagtggatc gcggaaggta tgttcggtct tctgctcaac agctccgtcg
     2941 tgcatgccgg tatgaacact acggagttgt atggcgacga aggtgttctg acgcagaact
     3001 ttggcgactc gcccgcgacg agtatggagt ggcccgacga cgcgaagccg gtacactgga
     3061 tgcgtgccga tcagggtgac cgcgtatggc agacgccgga tcttccgtta cagcggcaag
     3121 gcgaacgtat cgcgcaggtg ccgcgaccgt tcattgacgc ggtcgcacat ggcgcaccgg
     3181 tgatcgcgac cgccgaggaa gggcgtgtag cggtagagat ggtacttgcg gcatatgagt
     3241 c
//
LOCUS       DSCC01000339            2231 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1033174, whole genome shotgun sequence.
ACCESSION   DSCC01000339 DSCC01000000
VERSION     DSCC01000339.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1033174"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            781..>2231
                     /locus_tag="ENN56_04245"
     CDS             781..>2231
                     /locus_tag="ENN56_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HDS74732.1"
                     /translation="MEAHRSRIGEPLFSSRRSSPNAVRRCAMLRYLLLCGTILSTVIS
                     ARAVTFDLDYATFADLSGDTTRSYVEVYTRIDRWSLDTKTVLSARRFGLIQNAPDTVK
                     IGFTADVVVTKASGDTVAHERHIVDQPVDESYSGTAYDDLFTAWGFYLPPGDYVLGVD
                     IADHHSDRSERRDLPIHVRSYTTTEPILSDVELALSVAPVAAESRFAKHGLSVLANAR
                     RVFGYGAPVLYYYTELYHLEPGAEYTLNATLINESGDSVRQFGPFTVVPDERTSVQVK
                     GVNVMGYPDGEYTLRLMLSRGDDGVVVAERDAPFTVSRTPVATAITAERFKADVGWIA
                     TRAEMQTFDSATPLEKARLIEEFWLSRDPSPETLVNEAKIEHYRRLAFADDAFTDVGR
                     PGRDSDMGRIYIVYGPPSDIERFGAEANAKEYQIWHYERLESTPIFVFVDMVGTGIMT
                     LVHSTARDEARNPTWQTLVQPYEGTLQRQEQRM"
BASE COUNT          441 a          621 c          698 g          471 t
ORIGIN      
        1 aaacaccatc ttcgacgggt attcgtacga agtctgggat gccaccggtg gtaaccaccc
       61 gaccacggcg gatgttccgg catcggccga aacgatcgtg tggcttggct cctgcgattt
      121 ccagaacacc tcatggggtt ggtactgggg atacgagtac tcgttcggcc acagcgatct
      181 gtccgtatcg ttggggtata tcccgggtaa gtacgacgcg ccgaatatca tcaccgacgc
      241 ggtcgacgcc gggaagaacg tgtgggttgt gagcgaaaac tggcttgacg agctcgcttg
      301 ggaaggtgtc gcacacaccg gtagccttga gtacgactac cttggcttcc cgcttgacgc
      361 cccgacaacc ggtgcgtatt ggtcgtatta cgacgtaaac gccgtcgtat tcgaccttgc
      421 tcagtccggc acgtggacgg acctcgagtg gccgagcctg tcgacggata tcggtaaggt
      481 cgactacggt atcggcgact actacgattg gtcggttgac gcgctggatt cgctccgcgc
      541 ggacgtactg ccaatctatg aagtcaccgg catggagacc aacgaaggtc cgatcggccc
      601 ggtaccggct ggtgaaggtc cgtactacgt tggttggaca gtgctcgacg gcacaggcga
      661 cccgcaggtc gtcatgatga cgttcgacct gtactacttc tcgttggaaa gcgccacgaa
      721 agtgacccag aacgttctca gcggtttgtt cggtcattga gaaggcgttg aatagcgtgt
      781 atggaggctc accggtcgcg catcggtgag cctctgtttt cttcccggcg ctcatcgccg
      841 aatgccgtcc ggaggtgcgc gatgctccga tatctgcttc tctgcggaac gattctctcc
      901 acggtaatct ccgctcgcgc agtgacattc gaccttgact acgcgacgtt cgccgatctt
      961 tcgggtgata cgacgcgctc gtacgtcgag gtttacacgc gcatcgatcg ctggtcgctc
     1021 gatacgaaga cggtgctcag cgcgcgaagg ttcgggttga tccaaaacgc accggatacg
     1081 gtgaagatcg gattcacggc tgacgtcgtc gtcacgaaag cgtccggcga tacggtcgcg
     1141 catgaacggc acatcgtcga tcagccggtt gatgagtcgt attccgggac cgcgtacgac
     1201 gacctcttca ccgcgtgggg attctacctt ccaccgggtg actacgtcct tggtgtcgat
     1261 atcgcggatc atcacagcga tcgatccgaa cgacgcgatc ttccgatcca tgtgcgcagt
     1321 tataccacta cggaacccat ccttagtgac gtcgagcttg cgctcagcgt tgcgccggtg
     1381 gctgcggaga gccgattcgc gaaacacgga ctttcggtgc ttgcgaatgc gcgacgtgtg
     1441 ttcgggtacg gcgcgccggt tctctattac tacacggagc tctatcacct tgagccggga
     1501 gcagagtata cgctcaacgc gacgctcatc aacgaatcgg gcgattcggt gcggcaattt
     1561 ggtcccttca ccgtcgtgcc cgacgagcgc acgagcgtgc aggtgaaggg agtaaacgtc
     1621 atgggatatc cggatggcga gtacacgctg cggttgatgc tgagtcgtgg ggacgatggc
     1681 gtcgtcgttg ccgaacgcga tgcgccgttt accgtcagcc gcacgcccgt cgcaacggcg
     1741 attacagcag agcgattcaa ggcggatgtc ggctggattg cgacgcgcgc cgaaatgcag
     1801 acgttcgaca gtgcgacacc gttggagaaa gcgcgtctga tcgaggagtt ctggctatcg
     1861 cgcgacccgt cgccggagac gcttgtgaac gaggctaaaa tcgagcacta ccgtcgactc
     1921 gcattcgccg atgatgcgtt taccgatgtt ggtcgacccg gtcgtgacag tgacatgggg
     1981 cgtatctaca tcgtctacgg gccgccgtcg gatatcgaac gtttcggtgc cgaggcgaat
     2041 gcgaaagagt accagatctg gcactacgaa cggctggaaa gcacgccgat tttcgtattc
     2101 gtggatatgg tgggcaccgg tatcatgacg ttggtgcata gtaccgcgcg cgacgaggcg
     2161 cggaacccga catggcagac gttggtgcag ccgtatgagg gcaccttgca gcgtcaggag
     2221 cagcggatga t
//
LOCUS       DSCC01000340            2305 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031675, whole genome shotgun sequence.
ACCESSION   DSCC01000340 DSCC01000000
VERSION     DSCC01000340.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031675"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            226..1560
                     /locus_tag="ENN56_04250"
     CDS             226..1560
                     /locus_tag="ENN56_04250"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74733.1"
                     /translation="MSANRPNVILFMMDALRPDHLSPWGAGIHPTPNFERLASAGAFF
                     RNAFSHMPSSHPSRASILTGRDPHTHGVRINSRPLPSQETTLAQLLSAEGYLTATTRV
                     FSPGIGRGFVDQGLARDLSPFDRGPWDVVKELPEMATDEMETDVAKDVTALITWLQDY
                     SGDAEKRKHPFLLWADSEDTHEPWRPPKPFDALYTDPAYDGPDVSCPPMYSPKLSERE
                     KAHAVGLYDGVVALLDKHLGRLLDALERLDLSDNTLVVVLSDHGVHLGEHHLWRKPPT
                     LFDAVLRATLIMRLPGVIPAGFQSDGINLVNDVFATVLDVCGIDVPEAAKRQCTSLMP
                     LLSGAEQVREFIGLEFNAYKGTAAKGIRTPRWKYMHYRSVGDIPWDVGSPADIWRGNN
                     WAKTMLFDLANDPSESHDVRAEHPDIERQMRETLIDWLIESENDIPAPDPGD"
     gene            1602..>2305
                     /locus_tag="ENN56_04255"
     CDS             1602..>2305
                     /locus_tag="ENN56_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74734.1"
                     /translation="MAKRGDVYPAEEITLVDPSTGAEYVQMTNAPVHSVNLYFEYQSF
                     TPDDQTLLFLSQRVAMRGAPYDLFAADIESKRLRQLTDDEHPFSSPCAIPDEPRMLYG
                     VRGTSIVRMNVDDGSDEEIARWEGGRALGVGMMNGDGRVYVAVGTRTDGTSTLVRFDL
                     RTGDVQTFLDGIPMYHLTVNHTGTLVTFTGKMNGETGPMICTIDGENARFFGFRGFAH
                     GTFYGTTDLFQGTLLPP"
BASE COUNT          472 a          706 c          682 g          445 t
ORIGIN      
        1 gcccgtcgga cacgttcgag gagactttcg gcattcatgt acacgtccca atcgatggga
       61 aagagtttta cctgacgaca ccgtgtagaa ggtgcgcata gaccgttccc agcgccagtc
      121 tccccgtgcg tcgcatcgac ggatgagccg cgtatccttg ccccgtcaga ccgagttgta
      181 ctatgctatt cacactgtct gtttcaccat tgaaaggaac attttatgtc cgccaatcgc
      241 ccgaacgtca ttctctttat gatggacgcg ttgcgtccgg atcatctcag cccgtggggc
      301 gccggcatac atccgacgcc gaacttcgaa cggctcgcct ccgccggcgc gttctttcgg
      361 aacgcattct cgcacatgcc ctcgagccac ccgtcgcgcg cgagtatcct gacaggtcgc
      421 gatcctcata cccacggtgt ccgcatcaac agccgtccgc taccctcaca ggaaaccacg
      481 ctggcgcagt tactttcggc cgaggggtat ctcactgcga caacgcgcgt tttctctccc
      541 ggaatcgggc gtggcttcgt cgatcaggga cttgcgcgcg atctgagccc gtttgaccgt
      601 gggccgtggg atgtggtgaa ggaactcccg gagatggcca cggacgagat ggaaaccgac
      661 gtggcaaagg acgtcaccgc gctgatcacg tggctgcagg actactccgg tgatgcggag
      721 aagcggaagc atccgtttct gctctgggcc gacagtgaag acacgcacga accgtggcgg
      781 ccgccgaaac cgttcgacgc actgtatacg gatccggcgt acgacggacc cgatgtttcg
      841 tgtccaccga tgtattcgcc gaaactttcc gaacgcgaaa aagcgcacgc ggtagggctg
      901 tatgacggcg ttgtcgcgct actcgacaag cacctcggac ggttactcga cgcactcgaa
      961 cgcctcgatc tcagtgacaa cacgctcgtc gtcgtcctga gcgaccacgg ggtgcacctt
     1021 ggcgagcacc atctctggcg aaaaccgccg acgctcttcg acgcggttct ccgcgcgacg
     1081 ctgatcatgc ggctgccggg cgtgattccc gcgggctttc agtcggacgg aatcaacctc
     1141 gtaaacgacg tattcgcgac agtgctcgac gtctgcggga tcgacgtacc ggaagcggcc
     1201 aaacgtcagt gcacgagcct gatgccgctc ttgagcggcg cggagcaggt gcgcgagttc
     1261 atcgggctgg aattcaacgc atataagggc acggcggcga aaggcatccg cacgccccga
     1321 tggaagtaca tgcactaccg cagcgtcggt gacataccgt gggatgtcgg ttcgccggcc
     1381 gacatttggc gcgggaacaa ctgggcgaaa acgatgctgt tcgatcttgc gaacgatccg
     1441 agcgaatcac acgacgtgcg cgcagagcat ccggatatcg aacggcagat gcgcgagacg
     1501 ctcatcgatt ggctcatcga atctgagaat gacattccgg cacccgatcc cggcgactga
     1561 gcgtcgactt gtatcggttt ctccaacgaa ggaccgcgac catggcaaag cgaggcgacg
     1621 tctatcccgc cgaagagatc acgctcgttg acccgtcgac cggtgcggaa tacgtgcaga
     1681 tgacgaatgc accggtgcac agcgtcaatc tctacttcga ataccagtcg tttaccccgg
     1741 atgaccaaac gctgttgttc ctttcgcaac gtgtcgcgat gcgtggcgcg ccgtacgatc
     1801 tcttcgcggc ggatatcgag agtaaacggc tccgtcaact gactgacgac gaacatccct
     1861 tcagttcgcc gtgtgcgata cctgatgaac cacggatgct ctacggagtg cgcggtacgt
     1921 cgattgttcg catgaacgtg gacgacggat ccgacgaaga aatcgcgcgg tgggaaggcg
     1981 gccgtgcgct cggtgtcggc atgatgaacg gtgacgggcg cgtgtatgtg gcggtcggca
     2041 cccgcaccga cggcacatcc acgctcgtcc ggttcgatct gcgcacgggt gatgtacaaa
     2101 cgtttctcga cgggataccg atgtaccacc tcaccgtgaa ccacacgggc acgctcgtga
     2161 cgttcaccgg gaaaatgaac ggcgaaaccg gcccgatgat ctgtaccatc gacggtgaaa
     2221 acgcccggtt cttcggattc cgcggtttcg cacacgggac gttttacggg acaaccgatc
     2281 tcttccaggg gacgctcctt ccgcc
//
LOCUS       DSCC01000341            1675 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1049007, whole genome shotgun sequence.
ACCESSION   DSCC01000341 DSCC01000000
VERSION     DSCC01000341.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1049007"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            112..1443
                     /locus_tag="ENN56_04260"
     CDS             112..1443
                     /locus_tag="ENN56_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDS74735.1"
                     /translation="MNVSVIGTGYVGLVVGACLADTGHVVTCVDKVQEKIDRLNAGEI
                     PIYEPGLEELVRLNQKAGRLVFTTDLEDAVRKSLVNFIAVGTPQDEDGSADLANVLAV
                     ARAIGCAMETTDNDNRYKIIVNKSTVPVGTAYKVSEAVRAETANPFDVVSNPEFLKEG
                     AAVNDFMHPDRVVIGVDDPRVAEIMKELYSPFVRTGNPIMVMDVASAEMTKYAANTML
                     AVRISFMNEIANLCERVGADVELVRRGIGADKRIGPSFLFPGIGYGGSCFPKDVTALA
                     RTGQEYGCAMEIVEAAHRVNQRQKSVLIEKILAKYGDDLTGKRFAIWGLAFKAKTNDM
                     REAPAVDIVNAVLEHGATVSAHDPAAQHEAQALWGDRINLGNERYTVLDGADALVVCT
                     DWNEFREPDFTELVERMKEPVVYDGRNLYDPFAMSRRGIEYHCIGRAANAS"
BASE COUNT          363 a          455 c          513 g          344 t
ORIGIN      
        1 cacgttgtat ccttcccgtt gtcaatacaa ccggttttct ccaattttga tcatctttgc
       61 tataacgatg aagcacacgg gggagaaatc accggctaat tggaggagaa tatgaacgtc
      121 agcgtcatcg gcaccgggta cgttgggctt gtcgtcgggg catgcctcgc ggatacaggc
      181 cacgtcgtca catgcgtcga caaggtgcag gagaaaatcg accggctgaa cgcgggagag
      241 atcccaatct acgagccggg actcgaggag cttgttcggt taaaccagaa agccggtcgg
      301 ctcgtcttca ctaccgacct cgaagacgcg gtgcggaagt cactggtaaa tttcatcgcg
      361 gtcggtacgc cgcaggatga ggacgggtct gccgacttgg cgaacgtatt ggccgtcgct
      421 cgggcgatcg gatgcgcgat ggagacgacc gacaacgaca atcgctacaa aatcatcgtg
      481 aacaagagca cagttccggt gggaacggcg tataaggtat cggaagccgt gcgcgcggag
      541 acagccaatc cgttcgacgt ggtaagcaat ccggagtttc tcaaagaagg tgcggcggta
      601 aacgacttca tgcatcctga ccgcgtagtg atcggggtgg acgatccgcg cgtcgccgag
      661 atcatgaaag agctctattc gccgttcgtc cgtaccggta atccgatcat ggtcatggat
      721 gtcgcaagtg cggagatgac caagtacgcc gcgaatacga tgcttgccgt acgtatctcg
      781 ttcatgaatg aaatcgcaaa cctttgtgag cgggtcggcg ccgacgtaga gctcgttcga
      841 cgcggtatcg gtgcggataa acggatcggt ccgtcgttcc tcttccccgg tattggctac
      901 ggcggctcgt gttttccgaa ggatgtaact gcccttgcgc gcactggcca ggaatacggt
      961 tgtgcgatgg agattgtcga agccgcacac cgggtgaatc aacgacagaa gtccgtgctc
     1021 atcgagaaaa tccttgcgaa gtacggcgat gacctcaccg gcaagcgttt cgctatttgg
     1081 ggactcgcgt tcaaagcgaa gacaaacgac atgcgagaag ctcccgccgt agatatcgtg
     1141 aacgcggtat tggaacacgg tgcgacggtc tccgcgcacg atcccgccgc gcagcacgag
     1201 gcgcaggcac tctggggtga tcgcatcaat ctcggcaacg agcgttacac ggtgcttgac
     1261 ggggctgacg cgctcgttgt ctgcaccgat tggaacgagt ttcgcgagcc cgatttcacg
     1321 gagcttgtgg agcgcatgaa agagccggtc gtctacgacg gacggaacct gtacgatccg
     1381 ttcgccatgt cacgtcgtgg tatcgagtat cactgcatcg gccgcgcggc gaacgcgagc
     1441 tgacgctcgc gttcgttaag gcgaagcgac tttgtcgctt cgcccggtat cttcgatcga
     1501 agcgtcgatt tcgcggtacg actgaaaccg tgcgcgtctc ctcgccttcg gctcggagac
     1561 gtgggggcga agggcaacga atgcgtaagc attcgctctt cgccttgacg aactcgaacg
     1621 catgtgagag ctcgcgaatt cattcgccgg gcatgtcctc gccggaccat cccta
//
LOCUS       DSCC01000342            3590 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017496, whole genome shotgun sequence.
ACCESSION   DSCC01000342 DSCC01000000
VERSION     DSCC01000342.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017496"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..505
                     /locus_tag="ENN56_04265"
     CDS             <1..505
                     /locus_tag="ENN56_04265"
                     /inference="COORDINATES: protein motif:HMM:PF02780.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74736.1"
                     /translation="PGTPIMTNPDEIEPIEIGTWEQLRSGGDIAILAVGSMVEPALKA
                     AKLLEEKGVSSEIINARFVKPLDEKMLDELIARHDRWLTVEENVVAGGFGSAVLESLH
                     LRMSEDADAVRVRLMGIPDRFTEHGTREEVLVDVHLSAADIVETAIGFLSESHPDTIN
                     AVPAPLS"
     gene            378..1409
                     /locus_tag="ENN56_04270"
     CDS             378..1409
                     /locus_tag="ENN56_04270"
                     /inference="COORDINATES: protein motif:HMM:PF01513.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDS74737.1"
                     /translation="MGRAKKCLLTCTLVRQISSKPQSVFSLNRIPIQSMPCLLPFREG
                     EIGTSSEGLEPLVKTIGIIANTRKRRSAEIVQRIVTTADRFGMKVSIVGGDTAEFGVD
                     APSIDVAELSDRCDVVIACGGDGTILNAARLVAKNPKPILGVNLGALGFLAELSPDDV
                     ELGVEEIATDQYTVDRRMTLEAEVVGTNTRVTALNDILITKSNQSRIISLEMSERDRW
                     VNTYVADGIIIATPTGSTGYALAAGGPIVEPSVSCIVAAPICPHSLTVRPMIFADSVE
                     FDIRVTGAPESTIELSGDAQDFRPIESGQTVRIRRNKNDALLVHLRSSVSYYDILRQK
                     LHWGMDKSI"
     gene            complement(1751..2395)
                     /locus_tag="ENN56_04275"
     CDS             complement(1751..2395)
                     /locus_tag="ENN56_04275"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS74738.1"
                     /translation="MQAWNDAWKTSTGRADWLEPSSFIQEILPDIAQHDSKRCIDIGC
                     GPGRHAMYAARLGFHAIGVDAARSGVQYLRTWAATESASVDVSAADMNALPFENETFD
                     CAIAWNVIYHGTADVVVRAVDEVARILTPSGIFILTLISKRNIRYGVGTEIEPDTFVN
                     EDDPGETRHPHRFYGRTDAIRLLSAFDIIEAREVEQKTPESYHWEIACRKAHRT"
     gene            complement(2407..>3590)
                     /locus_tag="ENN56_04280"
     CDS             complement(2407..>3590)
                     /locus_tag="ENN56_04280"
                     /inference="COORDINATES: protein motif:HMM:PF02913.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDS74739.1"
                     /translation="RVLSVDYENRVAVVEPGVQNLQLSAITKPRGWHYAPDPSSQAAC
                     TLGGNVAENAGGPHCLKYGVTANHVLGLEVVLPNGELVELGGGTTDTEGYDLLGLFIG
                     SEGTLGIATKITLRLERLPPAVRTLLVDYDSLIAACESVSGIIADGIIPAALELMDRR
                     TIATVEDSVLAAGYPRDAEAVLVIELDGLEPSLDDQAGRITDICRTHGARTVRMALDE
                     TERAKLWAGRKAAFGAVGRLAPDVFVQDAVVPRTKLADVIARVHEICDREGVTVSNVF
                     HAGDGNLHPNVPFDSRDSAQVERIGRVMTEIMHVCVEAGGTISGEHGIGMDKRKYMDL
                     VFTPDALDLMARVRNVWNPKGLLNPGKILPERYRVVRNSDTEIDHNAPDGRAAVTGVT
                     V"
BASE COUNT          717 a         1018 c         1084 g          771 t
ORIGIN      
        1 accgggtacg ccgattatga cgaatcccga cgagatcgaa ccgatcgaaa tcggtacctg
       61 ggaacagctt cgtagcggcg gcgatattgc gatactcgcc gttggctcca tggtcgaacc
      121 agcgctgaaa gcagcgaagt tgctggaaga aaagggcgtt tcatcggaga ttatcaacgc
      181 cagattcgtc aagccgctcg acgaaaaaat gctcgacgag ctcatcgcgc ggcatgatcg
      241 gtggctcacc gtcgaggaga acgttgttgc cggcggattt ggctcggcgg tgcttgaatc
      301 gctccattta cggatgtcgg aagatgccga tgccgtacgt gtgcggctga tgggaatccc
      361 ggatcgcttc actgagcatg ggacgcgcga agaagtgctt gttgacgtgc accttagtgc
      421 ggcagatatc gtcgaaaccg caatcggttt tctctctgaa tcgcatcccg atacaatcaa
      481 tgccgtgcct gctccccttt cgtgaaggcg aaatcggtac gtcatccgaa ggattggaac
      541 cgcttgtgaa gacgatcggt atcatcgcga atacgcggaa acgacgtagc gcggaaatcg
      601 tacaacggat cgtcaccacg gccgaccggt tcggtatgaa ggtatcgatc gtgggcggtg
      661 acacggcgga gtttggcgtc gacgctcctt cgatcgatgt cgctgaactc tccgaccggt
      721 gcgacgtggt catcgcgtgt ggcggcgacg gcactatttt gaatgccgcc cgactcgtcg
      781 cgaaaaatcc aaaaccgatc cttggagtca atctcggtgc gcttgggttc ctcgcggagt
      841 tgtcacctga cgacgtagag ctcggcgtag aagagatcgc gacggatcag tacacggtcg
      901 accgacgcat gacactcgaa gcggaggttg ttggcacgaa tacacgcgtt accgcgctca
      961 acgatattct cattacgaaa agcaatcagt cacgcatcat ttccttggag atgagtgagc
     1021 gcgaccggtg ggtgaatacg tatgttgcgg acggaatcat catcgcgaca ccgaccggct
     1081 cgacgggata cgcgctcgcc gcaggaggac cgatcgtcga gccctcggtg agttgtatcg
     1141 tagccgcgcc tatttgcccg cattcgctga ctgtacgccc gatgatcttt gccgactcgg
     1201 tagagttcga catccgcgtg acgggcgccc cggaaagcac gatcgagctc tcgggtgatg
     1261 cgcaggactt ccggcctatc gagtcgggtc aaacggttcg gattcgtcgt aacaagaatg
     1321 atgcactgct cgtgcatttg cggagctccg tgtcgtatta cgacatcttg cgtcagaagc
     1381 tgcactgggg catggataag tcgatataac ggcgaacccg acgcttcgcg tcgggttcgt
     1441 taaggcgaag cgacggtgtc gcttcgcccc acgtcttcga gcgggatgag aggagacgcc
     1501 cggcgaatga attcgcgggc tcgggttgcg aagtctgcct acgcggactg gcggtacggt
     1561 taactcgcac ttccgagcct acgaaggcag gctttgtact tatagcgagc gacttcagtc
     1621 gcaacgcgaa cccttcgctg cgttacgcct tcgccccacg tcttcgagtg aaacgagagg
     1681 acgcgcgcgg tttcaaccgt tgcgcgaaac ggacgcaccg cgcgaactgt aatactcaac
     1741 ctcgacgttt tcaggttcga tgtgccttcc gacaagcgat ctcccagtga tagctctccg
     1801 gtgttttctg ctcgacctct ctcgcttcaa tgatatcgaa cgctgaaagc aagcgaatgg
     1861 cgtctgtgcg gccgtagaat cggtgtggat gcctcgtctc gccggggtcg tcttcgttga
     1921 cgaaagtatc gggctcgatt tccgtgccaa cgccgtaccg gatattccgc ttcgagatga
     1981 gcgtgagaat gaatatgccg ctcggagtga gaattcgcgc gacttcatcg actgcgcgga
     2041 ctaccacgtc ggcagtgccg tgatagatca cattccacgc gattgcgcaa tcaaacgttt
     2101 cattctcgaa tgggagcgcg ttcatgtccg cggcggaaac gtcgacggat gcgctttccg
     2161 tcgcggccca tgtacggagg tattgtacgc cggagcgcgc ggcatcaact ccgattgcgt
     2221 ggaacccgag gcgcgccgca tacatcgcgt gtcgaccggg tccgcagccg atatcgatac
     2281 agcgtttcga gtcgtgttgg gcgatatcag gaaggatctc ctgaataaac gacgacggtt
     2341 ccagccagtc ggcgcggcct gtcgaagtct tccatgcgtc gttccatgct tgcatacgag
     2401 cgccgtttac accgtcacgc cggttacagc ggctctaccg tcaggagcat tgtggtcaat
     2461 ctcggtatcg gagtttcgca cgacgcggta ccgttcgggc aatatcttgc cgggattgag
     2521 taatcccttc gggttccaga cattgcgcac gcgagccatg aggtcaagcg catccggtgt
     2581 gaaaacgaga tccatatact tccgtttgtc catgccgata ccgtgttctc cgctgatcgt
     2641 tccaccggcc tcgacgcaca cgtgcatgat ctcggtcatg acgcgcccga tacgctcgac
     2701 ttgtgccgaa tcgcggctgt cgaaggggac attcgggtgg aggttcccgt caccggcatg
     2761 gaatacgttc gacacggtca cgccctcgcg gtcgcagatt tcgtgcacac gagcgattac
     2821 gtctgcgagc tttgtgcgtg ggacgacggc gtcttgcacg aacacgtcag gtgcgagccg
     2881 tccgacggca ccaaaggcgg cctttctgcc cgcccatagc ttcgcccgct ccgtctcgtc
     2941 gagcgccata cggaccgttc gcgcgccgtg tgtgcggcag atgtccgtta tccgcccggc
     3001 ttggtcgtcg agactcggtt cgaggccgtc gagttcgatc acgagcacgg cttcggcgtc
     3061 ccgtggatac cctgcggcga gtacactatc ctccacggtc gcgatcgtgc ggcgatccat
     3121 cagttctaac gcagccggta tgatcccgtc ggcgataatg ccggaaaccg attcacacgc
     3181 ggcgatgaga gagtcatagt cgacgagaag cgtacgaact gcgggcggca aacgttcgag
     3241 tcggagcgta atcttcgtgg caataccgag ggtgccttca gatccgatga aaaggccaag
     3301 taggtcgtac ccttcggtat ccgtggtccc gccgccgagt tcgacgagct ctccgttggg
     3361 gaggacaact tcgaggccaa gtacgtgatt tgccgtgacg ccgtatttca agcagtgtgg
     3421 tccgcctgcg ttctccgcga cgtttccgcc gagtgtacac gctgcttggc ttgatggatc
     3481 gggtgcgtaa tgccatccgc gtggcttcgt aatcgcgctg agttgaagat tctgaacgcc
     3541 cggctctaca acggcgacac ggttttcgta gtcgaccgag aggacgcgat
//
LOCUS       DSCC01000343            4202 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1014202, whole genome shotgun sequence.
ACCESSION   DSCC01000343 DSCC01000000
VERSION     DSCC01000343.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1014202"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(2180..3778)
                     /gene="pckA"
                     /locus_tag="ENN56_04285"
     CDS             complement(2180..3778)
                     /gene="pckA"
                     /locus_tag="ENN56_04285"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDS74740.1"
                     /translation="MVGQAPDFGLENHGFFNLGTVHWNLSTSRLYEEAIRRREGQLAH
                     LGPLVVRTGHHTGRSPNDKFIVRESKTQDKVWWSKVNQEFDEQRWENMYRRLIAYMQG
                     RDCFVQDCLVGAHPDHELPIRIITEDAWQSMFARNMFVQIHDHEKLRTHKPEFTVIAI
                     PRFHAIPEIDGTRSEVFVIVNFATNTVLIGGTAYAGEIKKSVFTFMNYLLPQQEVLSM
                     HCSANMGEKGDTALFFGLSGTGKTTLSADKDRRLIGDDEHGWSDDGIFNFEGGCYAKM
                     IRLSADAEPEIYSTTRRFGTLLENVMMDADTRRLNLDDDSLTENTRGAYPLRHIEGAV
                     LPSHGPHPDNILMLTADAFGVLPPIAKLTPAQAKYYFLLGYTAKVAGTEKGITEPQAT
                     FSACFGAPFLALPPAVYAQLLGKRIREHKAHCWLVNTGWSGGPYGVGERMKIQLTRTL
                     IRAALSGDLADVETKEDPIFGLHIPVSCEGVSEAVLFPRNSWTDKDAYDVQAKDLAKR
                     FTENFKQYEESVEEDVRKAGPKAS"
BASE COUNT          881 a         1083 c         1257 g          981 t
ORIGIN      
        1 cggacagtgt tgcatgggct tccggcccga tcacgagtcg aaacggtcgc gcaatggcgt
       61 tcaactcagg tgtgtaccga atcgatgagc gtactgtgga ttcgcaggta tggagtccgc
      121 cggacggccg cctcgaggat ggtctcgaga tagccggcgt acccctgcaa gccgagagcg
      181 acgcgttgtg gacggtgagc attttcgact cgaaagctga cacggtgaaa cctcacctcg
      241 agatcgatgt gctcgccaca gacggtgagc ttattgggat tccgttcgat acgacgcgcg
      301 ttcgcctcgt cggagacact caacgtctat tgttcgagca cgggtatctc acgaaaactg
      361 ggtattatac cggcgtgacc gaaggcggag gaattccgat acgagagccg aacggcgaaa
      421 tcgatattcc catatacatc cgtcatgacg atggaaacga cgtgctttgg cttcttacgc
      481 acatgatcaa ccaaggtgtc gaaggtcacg ggtcagggca cggatttgtt cgggtggctg
      541 gtccaccgtc ggaggtcgtt atcggtgaag gttgggctga ggttacggac ggaagtatcg
      601 actggccggg cgggatatgg cctacgtggc gcggcgtgaa gatacgcgcg tcaatcgaac
      661 gcggtagtag gctcctcagt ttcgattcgt tcgaggcgcg tgttgcgaac ggaaccgttc
      721 gtgttgaaaa cgtaccggct gacgtgatgg gtgcgacacc attggtgatc gaagccgtcg
      781 gactcacgct tggtgtcata cagattacga cgccgcggga catcccgttc cgaattccgg
      841 gtggagtgcg tgtcggtgaa tggctcaacc tccgattcca tggaaacgaa cgtgtggatc
      901 gcttcacgat cgccggtcca gttgagcgtc cgctgtttgt cggcgagatt gtggcgtccg
      961 acggcttttt cacgtacccg ttcgaacaga cgaagagtaa ttccgattcg acagcgatgg
     1021 ggtttattgg aaacgcggaa tggcacgtcg acctcgttgt gggacgtaac cttgtttacg
     1081 aaacacgcac gacgcagagc ttgtggcgga gtctcttaca gaatccgatc gagcttcttg
     1141 gatcgatggc ggcggaaatc gaggcgagac ttgcagaagg cggccgaatc aagattcgtg
     1201 gagcgtacgc cgacaacagt ctgacggtga tctccgagaa ccttatcagc gagcaggcac
     1261 ggctctccgt gctcgatatt gagtttagcc aggacggccc gttggtattc gaatgggata
     1321 gccggctcaa tcaggaaccg attctacgcg gtcgtggcgt ggcgatgatc gaagtcgaat
     1381 cggggatgga gacgcttccg gaacctggtg atgaatcgtc gcgcggacga gagttccagc
     1441 gaatctacgc acgattggta agtgtcgatc cggttaccgg tattatccga gagggtggtc
     1501 gactcgacga gcttacggtg gaactcgata ccgacgagat acgcagtggt ctcagtgggc
     1561 aggaacggca actggcgatc cttgagatgc tcggttatta ccacgatccg tggcgcgaag
     1621 gtggatatga tagcaacggt gagcgcgcag ggttcgatcc gcgtgagctt gcgagcgcgg
     1681 gctaccggac cttggttcgt cggagtgaac agcaggcgtg gagatcggtg tttcttccgt
     1741 ttactcgaca gatgcgccga tttaccagac tcgacgtgat cgacgtacgc ccgtcgattc
     1801 tcttcaacgt attgaatcga gagttcgagc ttgacgggat ggctgcggag caggcgtatc
     1861 ttgcgtatct ccagggtaca aattggacgt ttggcgaata cttgtggggt ggattcctcc
     1921 tttcatacca cgggcagctt gaacttacga gcttgtcgtc tccgacactc ggtgcgcgac
     1981 atcaggtggt gctcgaatgg gcggttagtc cgcgaacccg cgtagagctg tcacgcgatt
     2041 tgaacgttcc attcggagag ccggatacac gagtcgggct ctcgcaccgt ttcgcgttcg
     2101 aatcctattg atcgtattgt taaaaaggcc gacatcgcat tgcgatatcg gcccttggat
     2161 tctcgatgag taggtcgggt tacgaagcct tcggtcccgc tttccgaaca tcctcttcga
     2221 cactctcttc atactgcttg aagttctcgg tgaatcgctt cgcgaggtcc ttcgcttgga
     2281 cgtcgtatgc gtccttgtcg gtccacgaat tccgtgggaa gagaaccgct tcggatacac
     2341 cttcgcacga aacaggaatg tgtaacccaa aaatcgggtc ttccttcgtc tctacgtcgg
     2401 cgagatcgcc ggaaagtgcc gctcgtatca gcgtgcgcgt gagttgaatc ttcatccgct
     2461 caccgacacc gtagggaccg ccgctccatc ccgtgtttac gagccagcag tgtgccttgt
     2521 gttcgcgaat ccgcttgccg agcagctgtg cgtataccgc tggcggaagc gcaaggaagg
     2581 gtgcgccaaa acacgcgctg aatgtcgcct gcggttcggt aattcctttc tcggtgcccg
     2641 caacttttgc ggtatagccg aggaggaagt agtacttcgc ctgagcgggc gtgagcttcg
     2701 cgattggagg tagcaccccg aacgcgtcgg ctgtcagcat gagaatgttg tccggatgcg
     2761 gaccgtggct cggaagaacc gcgccttcga tgtggcggag cgggtacgcg ccgcgggtgt
     2821 tttccgtcag gctgtcgtcg tcgagattca aacgacgtgt gtcggcgtcc atcatgacgt
     2881 tttcgagaag cgttccgaaa cgccgtgtcg tcgagtaaat ctccggctca gcgtcagctg
     2941 acaggcggat catcttcgcg tagcaaccgc cttcgaaatt gaagattccg tcatcgctcc
     3001 aaccgtgctc atcgtcaccg atcagtcggc gatccttgtc ggctgaaagc gtcgttttgc
     3061 ccgtgccgga cagcccgaag aaaagtgccg tatcgccctt ttcgcccatg ttcgcggagc
     3121 agtgcatcga aagcacttcc tgctgcggca ggaggtagtt catgaacgtg aatactgatt
     3181 tcttgatctc gccagcatac gccgtgcctc cgatgaggac ggtgttggta gcgaagttga
     3241 cgatcacgaa cacttccgag cgggtgccgt cgatttccgg aatcgcgtgg aagcgtggaa
     3301 tcgcgattac cgtgaattca ggcttgtgcg tacgtaattt ctcgtggtcg tgaatctgca
     3361 caaacatgtt gcgtgcgaac atgctttgcc atgcgtcttc ggtgatgatt cgaatcggca
     3421 attcgtgatc tggatgcgcg ccaaccaaac agtcctgtac gaagcagtcg cgtccctgca
     3481 tgtacgcgat gagacgacgg tacatgtttt cccaacgctg ttcatcgaat tcctgattga
     3541 ctttgctcca ccagaccttg tcctgcgtct ttgactcgcg aacgatgaac ttatcgttcg
     3601 gtgagcggcc agtgtggtga ccggtgcgga caaccaacgg tccaaggtga gcgagttggc
     3661 cttcgcgacg gcgaatcgct tcctcataga gcctcgacgt cgagaggttc caatgaaccg
     3721 tgccaaggtt gaagaatccg tgattctcaa gcccgaagtc gggagcctga cctaccatcg
     3781 tatagtctcc ttctgtggta ttcatcgcac tatcttgtcc acgtaagtgt catatggaca
     3841 ctaagtccgt tgtatgaaca taacacttca atacgctact gagaagtacc ggagttgctg
     3901 cttcgtttcc gggaacaaag aaaatcttcg gtatctgata ttcggatagt gtgttgagtt
     3961 gagaacgccg ctcgttgacc gtctccccga agatctcggt ctcttacgag tggaaatgta
     4021 cggtggttgc acgaacacgt tgcatgtgcg ctcttccacc gttaaagacg caggaatgaa
     4081 gataccaaca ggcagccggt cgtcaacagt ttccgcgtga gtaccttgac ggaatcgttt
     4141 tgccgatata tcttcataag agaatgaaga aatggaggcc ggaatgttat cgcagacacc
     4201 gc
//
LOCUS       DSCC01000344            1927 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040516, whole genome shotgun sequence.
ACCESSION   DSCC01000344 DSCC01000000
VERSION     DSCC01000344.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040516"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            279..1343
                     /locus_tag="ENN56_04290"
     CDS             279..1343
                     /locus_tag="ENN56_04290"
                     /inference="COORDINATES: protein motif:HMM:PF17179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74741.1"
                     /translation="MARQTDCRGLPMKKVMTRGEFWALMDRLVEERHVVGVVEKSPGH
                     YVFDRLKSIEQVANDYLPTILPPKKYLLPQTDPLFRYNRETSAMEPVEAFEEIVVFGV
                     RPCDITALAIVTERMLDGEPDEAVRARRDAMTIIGWDCKHPCDEHSFCESVGSLDPDY
                     GYDVLVRDIGGRKLVIETGSPKGDHLVKDVGRLFDEADKAMLASYMAQRNASFPKKLD
                     IPISDAPLLFTGTDENPFWDELADKCYSCGTCTTVCPTCYCFDVHEEIELDMKHGTRV
                     RTWDSCQLDEFAEVATGENFREERVERVRHRLQRKFNYQFTRTGQSHCVGCGRCARAC
                     LVGIDPVDVVNTLAQTQTIG"
     gene            1356..>1927
                     /locus_tag="ENN56_04295"
     CDS             1356..>1927
                     /locus_tag="ENN56_04295"
                     /inference="COORDINATES: protein motif:HMM:PF00970.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDS74742.1"
                     /translation="MIDSIYLPEMTEIVEAEMVSPHEKLFRLRFRDGRSLGHKPCQFV
                     EVSILGTGEAPISVSSPPYITDSFDLCVRACGNVTNALHRLGAGDTVGIRGPFGNGVD
                     LEEFVGRDLVFVAGGIGIVPLRSLIAEAVRRAGEFKSLTVVYGAKTPEELLFADEFAE
                     WERHANCLITVDRKAPGWNGHVGVVTTLLT"
BASE COUNT          442 a          523 c          544 g          418 t
ORIGIN      
        1 cattactgcg catcactcct cacgcgcgtt tcgttgcgta atggttctcg cgacgcgaca
       61 cggatcgcgc gacatgttta cctcgcgttc ttgacaattt ggcacgaata acactttcgc
      121 gacatcgtat cgttattggg taagtaattg tatccgcata atttatccgc atctcaatct
      181 cacccgtata cgccgttggc ataccatttg gattatccgt gtagcggtat cgaggcacgc
      241 ctgacgggga ggggtgcctc gaggtcggcg ttcgggcgat ggcacggcaa accgactgta
      301 gggggttacc gatgaaaaag gtcatgactc ggggcgagtt ttgggcgctg atggatcggc
      361 tcgtggaaga gcgacatgtc gtcggcgtcg tggaaaaatc ccccggacac tatgtctttg
      421 atcggctcaa gagtatcgaa caggttgcca acgattacct tcccacgatt ctcccaccaa
      481 aaaaatacct gctaccacaa accgacccgc tctttcgcta caatcgcgaa accagtgcga
      541 tggaacccgt cgaagcgttt gaagagatcg tcgtgttcgg tgttcgtccg tgcgatataa
      601 ccgcgctggc aatcgtgacc gaacgaatgc tcgacggtga acccgacgag gcggtgcgtg
      661 cgcgcagaga tgccatgacc atcatcggat gggactgtaa gcatccgtgc gacgagcaca
      721 gtttctgcga aagcgtcgga agcctcgatc cggattacgg atacgacgta ctcgtccgcg
      781 atatcggcgg acgaaagctt gtaatcgaaa ccggttcccc gaaaggcgat cacctcgtga
      841 aagacgtagg tcgcctgttt gacgaagccg acaaagcgat gctcgcgtcg tatatggcgc
      901 agcgtaacgc aagtttcccg aaaaagctcg atattccgat ttcggacgca ccacttttat
      961 ttaccggaac cgacgagaac ccgttctggg atgagcttgc cgataagtgc tactcgtgcg
     1021 gcacatgcac caccgtttgt ccgacgtgtt actgtttcga cgtacacgaa gagatcgagc
     1081 tcgacatgaa gcacggcaca cgtgtgcgga cttgggacag ttgtcaactc gacgagttcg
     1141 ccgaagtcgc gacgggcgag aacttccgcg aagaacgtgt cgaacgagtg cgacatcgtc
     1201 ttcaacgcaa gttcaattac cagttcacgc gaaccggtca atcgcattgt gttggatgcg
     1261 gcagatgcgc gcgagcgtgt cttgtaggca tcgatccggt cgacgttgtg aacacactcg
     1321 cgcaaacaca gaccatcggc tgagggaggt gaacgatgat cgattcaatc tacctaccgg
     1381 aaatgacgga aatcgtcgaa gccgagatgg tctcgccaca cgaaaaactg tttcgattgc
     1441 gtttccgcga tggccgatca cttgggcaca aaccgtgtca gttcgtcgaa gtatcgatcc
     1501 tcggcaccgg cgaagccccc atatcagtgt cgtcaccgcc gtatatcacc gattcgttcg
     1561 acctctgtgt ccgtgcctgc ggaaacgtga caaacgcgct gcacagactc ggagcaggcg
     1621 atacggtcgg tatacgcgga ccattcggca acggagtcga tcttgaagaa ttcgttgggc
     1681 gcgatctcgt gttcgtcgcg ggcggtatcg gaatcgttcc cctacggtcg ctcatcgctg
     1741 aagcggtccg acgcgcgggt gaatttaaga gccttacggt cgtctatgga gcgaaaacac
     1801 ctgaagaact actcttcgcc gacgaatttg ccgaatggga gcgtcacgcg aactgtctga
     1861 ttaccgtcga ccggaaagct cccggctgga acggacacgt tggcgtcgtt acgacgttgt
     1921 tgaccga
//
LOCUS       DSCC01000345            1734 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046724, whole genome shotgun sequence.
ACCESSION   DSCC01000345 DSCC01000000
VERSION     DSCC01000345.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046724"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..752
                     /gene="bshB1"
                     /locus_tag="ENN56_04300"
     CDS             <1..752
                     /gene="bshB1"
                     /locus_tag="ENN56_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HDS74743.1"
                     /translation="VLAIGPHRDDLELSCGGTIVRLLELGYSVGMADMTAGEMGTRGT
                     ADERAQEATAAAAALGVTYRANLGLPDGKLRDDDETRDRLIRVIRATRPKLVLCPYPD
                     DWRHPDHSAAGRVVPEAAFRSGFPKWETGQRHHRPLMTLHYMMHYEFEPSFIVDVSDQ
                     WEKKMEAVRCYRSQVHVAGHERATTEEQTYISRPEFSERIEARYRYFGSKIAAKYGEP
                     FWHPNPIRIDDPMQLVAGSIENFAQRGDRST"
     gene            complement(764..1537)
                     /locus_tag="ENN56_04305"
     CDS             complement(764..1537)
                     /locus_tag="ENN56_04305"
                     /inference="COORDINATES: protein motif:HMM:PF13561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS74744.1"
                     /translation="MRGLSEKTALVTGGTGLIGGATCVRLAEEGARVVVASRSYEKAE
                     EWCSAHQNAGTLTALALDVSSAEGIAAAFDRLRADNLHPTILVATAIDTRWHATPFER
                     LDYEHFAGIGRADVAGNFLVARRLVDDLRDDESASIVLFSSIYGSAGVEHSIYPEGMM
                     HSPVHYSATKAAVQGVTRWLAGYWGARGIRVNAIVSGGVRDENRQGDEFVERYNRRAM
                     LGRMGYPREIASAAAFLASDDASYITGTCLDVEGGLLAW"
BASE COUNT          339 a          539 c          519 g          337 t
ORIGIN      
        1 atgtactcgc tatcggaccg caccgcgacg acctcgagct gtcatgtggc ggcacgatcg
       61 ttcgtcttct tgagcttggt tactcggtcg gaatggcgga catgaccgcc ggagagatgg
      121 gaacgcgcgg tactgctgac gagcgcgcgc aagaggcgac cgcggccgcc gccgcgcttg
      181 gcgtcacgta tcgtgcgaac cttgggcttc ctgacggcaa actccgcgac gacgatgaga
      241 cgcgggaccg gctcattcgc gtcattcgcg caacacggcc gaagctcgtg ctctgcccgt
      301 atccggacga ttggcggcat ccggatcatt cggcggcggg tcgtgtggtt ccggaagccg
      361 cattccgctc cggctttccg aagtgggaaa cgggacagcg ccatcatcgg ccgctcatga
      421 cgctccacta catgatgcat tacgaattcg aaccgagctt tatcgtcgac gtatccgatc
      481 agtgggaaaa gaagatggaa gcggtgcgct gttaccgatc acaggttcac gttgcgggtc
      541 atgaacgtgc gacaaccgag gaacagacgt acatcagtcg cccggaattt tccgagcgta
      601 tcgaggcgcg atatcgctat tttggctcga agatcgcggc aaaatacggc gaaccgttct
      661 ggcatccaaa cccgatccgg atcgacgatc ccatgcagct cgtcgccggc agtatcgaga
      721 atttcgcgca acggggagac cgctcaacct gatgtgtcga gcttcaccac gcgagaaggc
      781 caccctcgac atcgaggcac gttccggtga tataggacgc gtcgtcggaa gcgagaaacg
      841 cggctgcgga ggcgatttct cgtgggtatc ccatgcgacc gagcatcgcg cgtcggttgt
      901 atcgttccac gaactcgtcg ccctgccgat tctcgtcgcg cacaccgcct gagacgatcg
      961 cgttcacgcg gattccacgc gcgccccagt atccggcgag ccaccgcgta acgccttgta
     1021 ctgcggcttt cgtcgccgag tagtgcaccg gcgagtgcat catgccctcg ggatagatcg
     1081 aatgctccac accggcactc ccgtagattg acgagaagag cacgatgctc gccgattcgt
     1141 catctcggag atcgtcgacg agtcttcgcg caacgaggaa attgcccgca acgtccgctc
     1201 ggcctattcc ggcgaagtgt tcataatcga gccgctcgaa cggcgtcgcg tgccaccgcg
     1261 tgtcgattgc cgtagcgaca agtatcgtcg ggtggagatt gtccgcgcgc agtcggtcga
     1321 atgccgccgc gataccttcg gccgaagaaa cgtcgagtgc gagcgccgtg agcgtaccgg
     1381 cgttctgatg ggcggaacac cattcctcag ccttctcgta agagcgcgag gcgacgacga
     1441 cgcgtgcgcc ttcctcggcg agccgaacac aggtcgctcc cccgatcagc cccgtgccgc
     1501 ccgttaccaa tgccgtcttc tcagataacc cacgcatgca ctttcccttc gttacgtttt
     1561 tcgccaaaca gaaatggaac gctgtatata taaggttcgc aacgtagcgc ctccggggaa
     1621 acgcgtgtcg agtgaaggcc gatggtcacc ggggaatcgt cgcgcgtttt ctgatgcgaa
     1681 tcgttcgccg catcgtcctc gcgttggcgc aacccgcgct gcgccgtact atta
//
LOCUS       DSCC01000346            2393 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030172, whole genome shotgun sequence.
ACCESSION   DSCC01000346 DSCC01000000
VERSION     DSCC01000346.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030172"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..725)
                     /locus_tag="ENN56_04310"
     CDS             complement(<1..725)
                     /locus_tag="ENN56_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74745.1"
                     /translation="MYVLDAVWKNPLAARRAERLAKAWPRVEVRTFSYADLPDIVVEE
                     GWDHKAKMGTMAEIPVPIPILGLFRFDEEAVARDETRMREAYRGNGGFPFSLAAGGGA
                     FVFFRSESRNFSCKSASEIRPNPEHICRPQWRIHQGIGCSHQCAYCSLGDFIITHVNT
                     EEYIERLGDLLAQNPWQKTWLYDDVMDVPTLEPELDTLAPLMRFFESTGDRYLIIHTK
                     TDRVDALIDAGAPRNTIIAWSLSG"
     gene            1189..>2393
                     /locus_tag="ENN56_04315"
     CDS             1189..>2393
                     /locus_tag="ENN56_04315"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01565.21,HMM:PF02913.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDS74746.1"
                     /translation="MVTNALTANLQYIVPESCVITDEASLRLYASDRSNLSSVPGIVV
                     LPATEDDLVKVVRALVEAELPLTIRGAGSGITGGAVPVEGGAIISTARMYDGIDVDPD
                     AGVVTAPAGARLIDVERAVAKHDCRLGDPISGYGVGTIGGAVARNSDGIGGRTGSLHG
                     LVRGIRGVLANGQVVEADTAGPHGQSLHGAALLIGSDGGLGVISSVRLAIQRKMPSDR
                     IVMAGFENVVTAAEAGAGLISAGACPVIADVFDRASWSETSPWPAGEGVDGMLLVWIS
                     GLEADVDDEAAWVIGVCRDYFALRANILDEDGFTVICGAWEDVIRANDNRPERVPIDL
                     SVPIHQIPELLRTLYARASEQSVSISGITRIARGTVNMYATVNPREDESVIRAGHYME
                     EMVEVAHDL"
BASE COUNT          476 a          698 c          733 g          486 t
ORIGIN      
        1 ccactcaggc tccacgcgat tatcgtgttg cgaggcgctc cggcatcgat tagcgcgtcg
       61 acgcgatcgg tcttcgtgtg aatgatgaga tatcggtcgc cggtgctctc gaaaaagcgc
      121 atgagcggtg cgagcgtgtc caattcgggt tccaatgtcg ggacgtccat gacgtcgtcg
      181 taaagccatg ttttctgcca cggattctgc gcgaggagat caccgagtcg ctcgatgtat
      241 tcctcggtat tcacgtgggt aataatgaag tcgccgaggc tgcaatacgc acactgatgc
      301 gagcaaccga ttccttggtg aatccgccac tgcggccgac agatatgttc gggattcgga
      361 cgaatttccg aggcgctctt gcacgagaaa ttccggcttt cggaacggaa gaaaacgaac
      421 gcgccacctc cggcggcgag gctgaacgga aaccctccgt tcccacgata ggcttcacgc
      481 atccgagtct cgtcgcgcgc gaccgcctcc tcgtcgaagc ggaacagccc gagtatcggg
      541 atcggtacgg gaatctccgc catggtgccc atttttgctt tgtgatccca gccttcctcg
      601 acgacgatat cgggcaggtc ggcgtaggag aacgtccgga cttcgacacg cggccacgcc
      661 ttcgcgaggc gttccgcacg gcgcgcggcg agcgggtttt tccagaccgc gtcgaggacg
      721 tacatacgcg gcggctcgaa catcggggtg cttgctacgg acacaaagga cctcatttcg
      781 gaaaacgcgg tgttgggacg caccttcttt ctcaggaaag tgtacggctg aacggcgtct
      841 tctcgcaaag ctcgaagcgg ttaggacgaa gcgcaaaacc aaatgccgac gcattcggtt
      901 tgcccgttcc gatttcaaac agacgggaaa cacatcgcta cgttaacaga cgtgggacgt
      961 ctctttgcta cgttagaaac cacaccccgg gcgaaggcgg tgcgccttcg ccttaacgaa
     1021 cgcggtgcgt cagcgcctta acgaacgcgg cgcgtcagcg ccgcgttcgc cgcctattgg
     1081 tctggatgac gtatttaccg attttcgata cggtctgaat cgaaatgacg cctcacgaac
     1141 ctccgtgttt gcgcctcgta tgagcgctac cgagacaccg atcggcacat ggtaacaaac
     1201 gcactcacag caaatctgca gtatatcgtg ccagaatcgt gcgtgattac ggacgaagcg
     1261 tcactccgtc tgtacgcatc cgatcgatcg aatctctcct cagtgcccgg tatcgtcgtg
     1321 ttgcctgcga cggaagatga tctcgtcaaa gtcgttcgcg ctctcgtaga agccgaatta
     1381 ccgcttacca tacgcggtgc cgggagcggg attaccggcg gagcggtgcc cgtcgaaggc
     1441 ggcgctatca tcagcacggc acggatgtac gacggtatcg atgtcgatcc tgatgccggc
     1501 gtcgtcaccg cccccgcagg cgctcggcta atcgacgttg agcgggccgt ggcgaagcac
     1561 gactgtcgac tcggcgatcc gattagtgga tacggcgtcg gcacgatcgg aggcgcggta
     1621 gcgcggaaca gcgacggtat cggcggtcga accggttcgc tccacggtct cgtgcgcgga
     1681 atccgcggcg ttctcgcaaa tggacaggtg gtcgaagcgg atactgcggg tccacatggg
     1741 cagtcgctcc acggcgctgc gctcttgatc ggcagcgacg gcggtctcgg tgtgatttca
     1801 tcagttcgcc ttgcgattca acgaaaaatg ccctccgacc gcatcgtcat ggccggtttt
     1861 gagaatgtcg ttaccgcagc cgaggccggc gcggggctga tttccgccgg agcatgtccg
     1921 gtaatcgctg acgtcttcga ccgagcgagt tggtccgaga cttcgccatg gcccgccggc
     1981 gaaggcgttg acggcatgct gctcgtctgg atctcaggcc tcgaagcgga tgttgacgat
     2041 gaggcggcgt gggtaatcgg cgtctgtcgc gactacttcg cgttgcgtgc gaacatcctc
     2101 gacgaagacg gattcacggt gatttgtggc gcgtgggagg atgtcatccg cgcgaacgac
     2161 aaccgacccg agcgcgtgcc gatcgacctt tcggtgccga ttcatcagat accggagctc
     2221 ctccgaacgc tttatgcccg tgcttccgaa caaagcgtct ccatttcggg cataacgcga
     2281 attgcgcgcg gcacggtgaa catgtacgcg acagtcaatc cgcgtgaaga cgaatcggtc
     2341 atacgcgccg ggcattatat ggaagagatg gtggaggtcg cgcacgatct cca
//
LOCUS       DSCC01000347            2835 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1024149, whole genome shotgun sequence.
ACCESSION   DSCC01000347 DSCC01000000
VERSION     DSCC01000347.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1024149"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(106..501)
                     /locus_tag="ENN56_04320"
     CDS             complement(106..501)
                     /locus_tag="ENN56_04320"
                     /inference="COORDINATES: protein motif:HMM:PF01050.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDS74747.1"
                     /translation="MKHWAMIDEVPTENVRASTTSGLRATWLCDWNEGFAPIGNTERV
                     RITLQQYTPGGDHKPHQHESLEQVYIMVKGTGRMHIGDEEFDAVPGMMIYVPAKTDHY
                     IVNIGDDELVHYLININLGDEKPPSDPRA"
     gene            complement(537..1361)
                     /locus_tag="ENN56_04325"
     CDS             complement(537..1361)
                     /locus_tag="ENN56_04325"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00190.20,HMM:PF07883.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDS74748.1"
                     /translation="MSICVRPGEVPLTPGLQQTEAGGLNTTGLMDENLLAPLHGTDTV
                     SKTAKNAGFVGTKRLTMIRQHYAPGGTHLPHKHDLAEQTYYLESGNAQVRVGEDAYDL
                     VPGSIVYIPPRVEHEFKNNSDESCVNILISVIVDEGDLTTSSKLRGFCVQPGDVKHVP
                     GIATKKDGTPNTTNLMDESLLHRRKENGTVRLGMIRQWYGVGGRHFPHRHSDNEQAYY
                     LAKGNARVRVGDETFDMTRGTVVYLPSNIEHELLNPGPDTCANVLIGVQLNTGDVA"
     gene            1506..2249
                     /locus_tag="ENN56_04330"
     CDS             1506..2249
                     /locus_tag="ENN56_04330"
                     /inference="COORDINATES: protein motif:HMM:PF02633.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74749.1"
                     /translation="MADEVRYEYLRPAQIVERRSTFPLAFVPLGTLEWHGPQNPVGLD
                     GLKAHALCVRAAEKCGGLVFPVVWYGEHRESHLMEINAGSGAAIRERMGFANEDFALG
                     YMGGGTIFTQAEDYLRLLWKIASQVKSLGFRALIFFNGHYPLTHYGRFVEHLARRHLS
                     LPIWAGHEGELLAEVGNPGHGDHGGKWETSLMMSIDAARVDLESLRASGEFVGCGADA
                     LESSVDQGDIWTREITNALIAKANSLLDT"
     gene            complement(2322..2528)
                     /locus_tag="ENN56_04335"
     CDS             complement(2322..2528)
                     /locus_tag="ENN56_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74750.1"
                     /translation="MPLGDGKGPPDGSGPLTGRKRFADACRAFTGSSTGTQTTSSGRL
                     ILTLIAGAVLLAGWLMTRSSDRER"
BASE COUNT          617 a          763 c          779 g          676 t
ORIGIN      
        1 gtgtttctgc ataacgtcct tactacctct tcgcacgcgc gggaatatgc gaaggcgcga
       61 gaacgactga gattcgtccc cgcatcttcg taatcgtcat cgtatttagg ctctcggatc
      121 cgaaggcggc ttttcgtctc cgaggttgat gttgatgagg taatgcacga gctcgtcgtc
      181 cccgatattg acgatataat ggtcggtttt ggcgggaacg tagatcatca tgccgggcac
      241 cgcatcgaat tcctcgtcac cgatgtgcat ccgaccagtc cccttcacca tgatatagac
      301 ctgctctaac gactcgtgtt ggtgcggctt atgatcgcca cccggtgtgt actgctgaag
      361 cgttatacgt acgcgctcgg tattcccaat cggagcgaaa ccttcattcc agtcgcagag
      421 ccacgttgcg cgcaaccctg atgttgtcga cgcacgcaca ttttccgtcg gtacttcatc
      481 gatcattgcc cagtgtttca tctcgtgctc ccttcgattg aagctcactc actctgtcac
      541 gcgacatcgc ccgtattcaa ttggactccg atgaggacgt ttgcgcaggt atccggaccc
      601 ggattcagca gttcgtgttc gatgttcgac ggaagataca caacggttcc tcttgtcata
      661 tcgaacgttt catctccgac acggacacgc gcattccctt tcgcaaggta atacgcctgt
      721 tcattgtcac tgtgccggtg tggaaaatgc cgtccaccga caccgtacca ttgacgaatc
      781 attccgagtc gaacggtgcc attttctttc cgccgatgga gcaacgattc gtccatgaga
      841 ttcgtcgtat tcggtgtgcc gtctttcttc gtcgcgatgc cggggacatg cttcacgtcg
      901 ccgggttgaa cgcagaaccc tctgagcttc gatgaggttg taagatcgcc ttcgtcgaca
      961 atcacactga tcagaatgtt tacacacgat tcgtcactgt tgttcttgaa ttcgtgctcg
     1021 acgcgcggag gaatgtacac gatgcttcca ggcacgagat cgtacgcgtc ctcgccgaca
     1081 cgcacttgcg cgtttcccga ttcgagatag tatgtctgct cagcgagatc gtgcttgtgg
     1141 ggaaggtgcg tgccaccggg cgcgtagtgc tggcgaatca tggtgagccg tttggttccg
     1201 acgaaaccgg cgtttttcgc ggttttcgac accgtgtcgg tgccgtgcaa cggtgcgagc
     1261 aggttttcgt ccatcaatcc ggttgtattg agtccgccgg cttctgtctg ttgaagaccc
     1321 ggtgtcaatg gaacctctcc gggacgcacg caaattgaca tggttcactc cttacgtttc
     1381 actcgcgtat cgaggacatt cttcacagag aagatcgaac gtagattgaa cgaaccaagg
     1441 ccagatttag aactaccaaa gcgatttatg ccggaaaaat ccataaactt gggagagata
     1501 atcggatggc agacgaagtg cggtacgaat acttacgacc ggcgcagatc gtagagcgga
     1561 gatcaacgtt tccactggca ttcgttccac ttggaacgtt agagtggcac ggtccacaga
     1621 atcccgttgg actcgacgga ctgaaagcgc atgcgctctg tgttcgcgct gccgagaaat
     1681 gcggtggact tgtgtttccc gtcgtttggt acggtgagca ccgcgaatcg catctgatgg
     1741 aaatcaatgc cggctcaggt gcagcgattc gtgagcgtat gggatttgca aacgaagact
     1801 tcgcactggg atacatggga ggaggaacga tctttaccca ggcggaggat taccttcgcc
     1861 tgctctggaa aatcgcgtcg caggtcaaat cacttgggtt tcgcgcgctc atattcttca
     1921 acggccatta cccactcact cattacgggc gcttcgttga gcatcttgca cgtcgtcatc
     1981 tcagtctacc gatatgggcg gggcacgaag gtgaattgct cgcggaagtg gggaatccgg
     2041 gtcacggtga tcatggggga aaatgggaaa catcgctcat gatgagcata gacgcagctc
     2101 gtgtcgacct cgagtcgctg cgcgcgtccg gagagttcgt tggatgcggc gccgacgctc
     2161 tcgagagctc ggtcgatcaa ggcgatatct ggacgcgcga aatcaccaac gcactgattg
     2221 cgaaggcaaa ttcgttactc gatacgtgat atatgtcgag ggagatactt cgcgtcgcgc
     2281 gaaacaattc gcgttgcgcg agatatttcg cggtcgcgcg gctaacgttc tcgatcggat
     2341 gatctcgtca tgagccatcc ggcgagaaga accgctccgg caatcaacgt gagtatcagt
     2401 cggccagaag aggtagtctg ggtgcctgtc gacgagcccg tgaacgcgcg acacgcgtct
     2461 gcgaaccgtt ttcgaccggt cagcggtccg gaaccatccg gcgggccttt tccatcacca
     2521 agcggcatac aatcctccat gcgcgattct ttacgaatta ctcttgatac gctctctcaa
     2581 cagtacgata cgcagacgcc cgtgaggagt tccgtggact tcgatgtgcg agatactttg
     2641 cccggaacgt atcagcggct accttcgtga gtcactcgac agttccacga tagcaaggaa
     2701 acgcacaatg tacaacatca ggaaaatcgc aagcgacatt cccggctccg aaggaccggt
     2761 gctcaacacc aaaggtgagt tcttcatggt tgcgccgaat cgaggcatga tcgtgaaggt
     2821 agaggcgaac ggaac
//
LOCUS       DSCC01000348            2448 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029283, whole genome shotgun sequence.
ACCESSION   DSCC01000348 DSCC01000000
VERSION     DSCC01000348.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029283"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            392..1150
                     /gene="fabG"
                     /locus_tag="ENN56_04340"
     CDS             392..1150
                     /gene="fabG"
                     /locus_tag="ENN56_04340"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDS74751.1"
                     /translation="MLQNFGSLSDRVVLVTGGSGDIGRALCEEFAKAGATVAMTSRRG
                     TEALGPAIAAVARAGAESLPFACDVRDREQIESTVSAIVERTGKLDILVNNAGITKDT
                     LLMRMSDEQWNDVIQTNLTSVFLCTQAAIKPMVKARYGRIISITSIVGQIGNAGQANY
                     ASAKAGIIGFTKTVAKELASRNITANAIAPGYIQSRMTDEIPEKAKQAFLDAVPLKRP
                     GLPAEVAYAAVFLASEQAAYITGQVMNIDGGLVM"
     gene            1233..1475
                     /locus_tag="ENN56_04345"
     CDS             1233..1475
                     /locus_tag="ENN56_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDS74752.1"
                     /translation="MMAVKDEVKELVVEQLGVSEDEVSDDSRFIEDLGADSLDTVELV
                     MAFEDRFNIEIPDEDAEKIRTVGDAVRYLEEKKGAE"
     gene            1573..>2448
                     /locus_tag="ENN56_04350"
     CDS             1573..>2448
                     /locus_tag="ENN56_04350"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDS74753.1"
                     /translation="MSKNKRRVVVTGFGAVTPIGKTLEEFWAALQRGDSGIGPITRFD
                     ATDYSTKIAAEIKDFDPTVRIDRKEARRMDPFVQYAVVAALEAIEHSGLNLSIEDADR
                     IGVIIGSGIGGLTTIESEYRNLHERGVRRVSPFMIPMLIADMASGMVSILTGVKGPNY
                     ATVSACASSAHAIGDSIMLIRDGKADVMIAGGAEASLTPLGMAGFCQARAMSTRNDEP
                     QRASRPFDADRDGFVCGEGSGVLILEELERAQSRGATVYGEVIGFGYSADAYHLTAPP
                     EGGEGMAKSMREAILD"
BASE COUNT          576 a          637 c          726 g          509 t
ORIGIN      
        1 caccgagcgt agagcgaaac gaatcgttcc gctctcggta agtggcgcgt ttcattctcc
       61 gctgatggaa tcggctgcgg agcgcctccg cgatgcaatc gcgcatacag agattcggca
      121 accgcgcgtt cccgtggttt caaacgtgac tgcgaaaccc actactgatc cagtcgagat
      181 tcgcgatctg cttgttcgtc agttgacgtc gtcggttcaa tggactgcaa gcgttcgatc
      241 tctcgttgct cttggggtga cgcgcgcgta tgaagttggt ccgggtaaag tgctgcaagg
      301 cctcgcgcgt cggatcgatc gatcgattca ggtctctggt gcaggtacgg cggctgagct
      361 tggagcgttg cggaaggagt aagccttgag catattacag aactttggtt cgctttccga
      421 tcgcgtcgtt ttggtgacgg gcggttcggg tgacatcggt cgcgcgctct gcgaggagtt
      481 cgcgaaagcc ggtgcaacgg tcgccatgac aagccgccgc ggcaccgaag cactcggacc
      541 ggcaatagca gccgtcgcgc gagcgggtgc cgaatcacta ccgtttgcgt gtgacgtccg
      601 cgaccgggag cagattgagt cgaccgtgtc cgcgattgtc gaacggactg gcaagctcga
      661 tattctcgta aacaacgccg ggattacaaa agacacactg ctcatgcgga tgagcgatga
      721 gcaatggaac gacgtgattc agacgaatct tacgagcgtg ttcttgtgta cgcaggcggc
      781 gatcaagccg atggtgaaag cccgatacgg tcgaatcatt tccataacat cgattgtcgg
      841 acagatcggc aacgccggac aagcgaacta cgcaagcgca aaagcaggca ttatcggttt
      901 tacgaaaacc gttgcaaaag aactcgcatc gcggaatatt acagcgaacg ccatcgcacc
      961 cggatatatc caatcgcgca tgaccgatga gataccggag aaggcgaaac aggcctttct
     1021 cgacgcggtg ccgctgaaac gccccggtct accggccgaa gtagcgtatg cagcggtgtt
     1081 tctcgcatcg gaacaagcgg cttatattac cggccaagtc atgaacattg acggcggcct
     1141 tgtcatgtaa ccaaacccgg taacccacga ctttgacgac gtgttgaaga agtgttccgg
     1201 aatacaaccg atctcccgat attgaggagg aaatcatggc ggtaaaagac gaagtaaaag
     1261 agctcgtcgt cgaacagctc ggcgtttcgg aggatgaagt cagcgacgat tctcggttta
     1321 tcgaggatct cggcgccgat tcacttgata cggtcgaact tgtcatggca ttcgaagacc
     1381 gattcaacat agaaatcccc gacgaagacg ccgaaaagat tcgcacggta ggtgacgcgg
     1441 tgcgctatct ggaagagaaa aaaggcgccg agtaatcgga gtctgcttcg aacgcaacat
     1501 gccggttcga cggtacgatc gctgtgaaaa ccaacgatcg taccgtcgga aatacctgtg
     1561 agggaatctc gcgtgagtaa gaacaagcgt cgagtcgttg taacgggttt tggcgcggta
     1621 acgccgatag gaaaaactct cgaagagttt tgggctgcgt tacaacgtgg cgattccggc
     1681 attggtccga ttacgcgatt tgatgcgacc gactattcga cgaaaatcgc cgccgaaata
     1741 aaagatttcg atccgacggt gcgaatcgat cgaaaagaag cccgccgcat ggatccgttc
     1801 gtgcaatatg cggtcgttgc agcactcgaa gcaatcgagc attcgggtct caatctctcc
     1861 atagaagacg ctgatcgtat tggcgttatc atcggctcgg gaatcggtgg tctgacgaca
     1921 atcgaatcgg agtaccgcaa ccttcacgag cgtggcgtac gacgagttag ccctttcatg
     1981 atccccatgc tcattgctga catggcatcc ggcatggtga gtatcctgac cggtgtaaaa
     2041 ggcccgaatt acgcgacggt atcggcgtgt gcatcgtccg cacacgctat cggtgactcg
     2101 atcatgctca tccgcgacgg aaaagccgat gtgatgattg ccggcggagc tgaagcgtcc
     2161 ctgacaccac tcggaatggc gggtttctgt caagcgcgcg ccatgtcgac gcgtaacgat
     2221 gagccgcaac gggccagcag gccattcgat gcggatcgtg acggtttcgt ctgcggcgaa
     2281 ggttcgggcg tccttatact tgaggaactc gagcgggcac aatctcgcgg cgcgacggtg
     2341 tatggcgaag tcatcggttt cggatattca gccgacgcgt atcaccttac cgccccgccg
     2401 gaaggtggcg aaggtatggc gaaatcgatg cgtgaagcca tactcgac
//
LOCUS       DSCC01000349            1510 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1056290, whole genome shotgun sequence.
ACCESSION   DSCC01000349 DSCC01000000
VERSION     DSCC01000349.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1056290"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1510)
                     /locus_tag="ENN56_04355"
     CDS             complement(<1..>1510)
                     /locus_tag="ENN56_04355"
                     /inference="COORDINATES: protein motif:HMM:PF08264.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDS74754.1"
                     /translation="VDEWFISMDELRHEIADVTRKINWMPSYGLEHELDWLKNMGDWM
                     ISKKRYWGLALPIYPCRKCGNVDVVGGYDELKERAVEGWDEFDGHSPHRPWIDKVKIA
                     CSSCGEPVSRVKDVGNPWLDAGIVPYSTMGYIEDRENWKKWFPADFITESLPGQFRNW
                     FYSLLAMSTVLEKREPFRNVLGFASLRDIDGEEMHKSKGNAIWFDDAAEKMGAETMRW
                     LYARSNPYNNINFGFESAEEPLRHLTTLWNTYKFFVTYAKIDNYDPDAKQILYADLAE
                     IDRWILARLNALIVEARRALDGFNAMALMRGAEAFIDDLSNWYLRRSRDRFRQMGEAG
                     DPAAHQTLYTVLVTFVRLLAPVIPMYTEELYENLAAWRNGQPESVHLTAYPEPVAEWE
                     NPQLVERMAVARKVVEVALAARNEAKVKVRQPLAKMTVGVDAGAKRDAVIVYRDVILD
                     ELNVKDLAASMGMDSMTQLVARPNFRALGPRFGKRANPIGNAIRALDQDRVAK"
BASE COUNT          296 a          470 c          406 g          338 t
ORIGIN      
        1 gcttcgctac gcgatcctga tccaacgcgc gaatcgcgtt gccgatgggg ttcgcacgct
       61 tcccgaagcg cggtccgagc gcgcggaagt tcggacgggc gacgagctgg gtcatcgagt
      121 ccattcccat acttgccgca aggtctttga cgttcaattc gtcgagaatc acatcacgat
      181 atacgatgac tgcatcgcgt ttcgcgccgg cgtcgacgcc gacagtcatc ttcgcaagcg
      241 gctgccgcac cttcaccttc gcttcattcc gagcggccaa cgccacttcg acgactttcc
      301 gtgcaactgc catacgctcg acaagctgtg ggttttccca ctcggcgacc ggctccggat
      361 acgcggtcag atgcacactt tccggctggc cgtttcgcca tgccgcgagg ttctcataca
      421 attcctcggt gtacatcgga atcaccggcg caagcagtcg gacgaatgtc accaacacgg
      481 tatagagcgt ctgatgggcg gccggatcac ccgcttcgcc catctgccgg aagcgatctc
      541 ggctgcggcg gagataccaa ttcgacagat cgtcaatgaa cgcctcggcg ccgcgcatga
      601 gcgccattgc gttgaatccg tcaagcgcgc gccgtgcttc gacgatcagc gcgttgagcc
      661 gcgcgaggat ccatcggtcg atctctgcga gatccgcata gagaatctgt ttcgcgtccg
      721 gatcatagtt gtcgatcttc gcgtaggtga caaagaactt ataggtattc cataacgtcg
      781 taaggtgtcg aagaggttcc tcggcggatt cgaaaccgaa gttgatgttg ttgtagggat
      841 tactccgcgc gtaaagccac cgcatggtct cggcacccat cttctctgcg gcgtcgtcaa
      901 accagatcgc gttgccttta ctcttgtgca tttcctcgcc gtcgatatcc cgcaggctcg
      961 cgaatccaag cacattgcgg aacggttcgc gcttttccag taccgtgctc atcgcgagga
     1021 ggctgtagaa ccaatttcgg aattgtccgg gcaaactctc ggtgatgaag tcggcgggga
     1081 accatttctt ccaattctcg cgatcctcga tgtatcccat cgtcgaatac ggcacgatac
     1141 ccgcatcgag ccatgggttg ccgacatctt tcacacggct gaccggctca ccgcacgacg
     1201 aacatgcgat cttcaccttg tcgatccacg ggcggtgcgg cgaatggccg tcgaactcat
     1261 cccacccctc gacggcgcgt tccttcaact cgtcgtatcc accgacgacg tcgacattcc
     1321 cgcatttacg gcatgggtaa atgggcagtg cgagtcccca ataccgcttc ttggagatca
     1381 tccagtcgcc catgttcttg agccagtcga gttcgtgctc gaggccatac gagggcatcc
     1441 agttgatctt ccgcgtcaca tcggcaattt cgtgacgaag ctcgtccatg ctgatgaacc
     1501 actcgtcgac
//
LOCUS       DSCC01000350            2398 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030094, whole genome shotgun sequence.
ACCESSION   DSCC01000350 DSCC01000000
VERSION     DSCC01000350.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030094"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1542
                     /locus_tag="ENN56_04360"
     CDS             <1..1542
                     /locus_tag="ENN56_04360"
                     /inference="COORDINATES: protein motif:HMM:PF12905.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74755.1"
                     /translation="LDAVGPGVYHVIPSRQGFLLPPDWPHPVDWMWIGLANTTGIYLP
                     MWGAIRPGDSYVAILETPFDGGFQLDHPAGGPTRICAKWHPSLGSIRYARQIRYSLLA
                     GAGFNEIIGRYREYVRADGRLKTLEAKATEVPRVRELEGTTIATTHTLYHNQPDAHYY
                     DPNNAEQNHRLNPFADCAEGLRRFAKRYDSDRVVVHLDGWGTRGYDNMHPDILPPCPE
                     AGGWEGFKSVADVCDENGWLFATHDNYIDYYLDAATYTDDLAVLDENGEIPKKAWWAG
                     GAQAFLCEQNALGYVQRNFEEILRQGVKLTSTYIDVFSIIEPFECYHPDHPETREESL
                     RARAACFNWVRSKGIVLSSEEPTDWSIPYIDFCYWAPLQQNKGLLEGELAGIPIPLFN
                     GVYHDCIVTPWQLTKADSVSDIDQFLYALSFGGCPMVGNPDRKDAFPAGTLERAKILA
                     DAHRQTGFAAIERHDFLSPDGSQRRTTFANGLAVEIDLRKRQYRFEGFKRMKKKWSDV
                     PGAGA"
     gene            1701..2045
                     /gene="erpA"
                     /locus_tag="ENN56_04365"
     CDS             1701..2045
                     /gene="erpA"
                     /locus_tag="ENN56_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDS74756.1"
                     /translation="MTEAVETLVNITEPAANKIKELLEQEDNSEYCLRLQVVGGGCSG
                     FQYRLAFDDQIADDDEIVDYDGIKVVVDPLSARYVEGARIDYVETEMGAGFKIDNPNV
                     QTTCGCGHSFSA"
     gene            2234..>2398
                     /locus_tag="ENN56_04370"
     CDS             2234..>2398
                     /locus_tag="ENN56_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005703748.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDS74757.1"
                     /translation="MPATNQHIHLTGICGTAMGALALMLKERGYHVTGSDAQAYPPMS
                     TVLAENGITVI"
BASE COUNT          536 a          623 c          721 g          518 t
ORIGIN      
        1 ctggacgcgg tcggaccggg agtgtatcac gtcattccga gtcgacaagg gtttcttctg
       61 ccgcccgact ggccacatcc ggtggattgg atgtggatcg ggcttgcgaa cacgacgggt
      121 atctaccttc cgatgtgggg agcgatacgg ccgggtgatt cgtatgtagc gatacttgag
      181 acgccgtttg atggaggatt tcaactcgat catcctgcgg gtggaccgac caggatctgt
      241 gcgaaatggc acccttcgct cggaagcatt cggtacgcgc ggcaaatccg gtactcgttg
      301 cttgcaggcg caggtttcaa cgaaatcatc ggccggtatc gcgagtatgt acgcgcagac
      361 ggacgcctca agacactcga agcgaaggcg acggaagttc ctcgtgtgcg cgagctcgaa
      421 ggaacgacga tagcgacgac gcatacactg taccacaatc aaccggacgc gcattactac
      481 gatccaaaca acgcggagca gaatcatcgc ctgaacccct tcgccgactg cgcggagggg
      541 ctgcggcgat ttgcgaaacg gtatgattcg gaccgagtag tggtgcatct cgacggatgg
      601 ggaacgcgcg gatacgataa catgcatccg gatatcctcc ctccgtgtcc tgaagccgga
      661 ggatgggaag gtttcaagtc tgttgccgac gtgtgcgacg agaacggttg gcttttcgca
      721 acgcacgaca actacatcga ttactatttg gacgcggcaa cctataccga cgacctcgca
      781 gtgttggacg agaatggcga gataccgaag aaggcgtggt gggccggtgg cgctcaggca
      841 tttctctgcg agcagaatgc actcgggtac gtccagcgca acttcgaaga gattctgcga
      901 cagggcgtga agcttacgtc gacgtacatc gacgtgttca gcattatcga gccgtttgag
      961 tgctatcacc cggatcatcc cgaaacgcgg gaagagtcgc ttcgagcgcg cgccgcgtgc
     1021 ttcaattggg tgcgttcaaa gggcatcgtg ttgagttccg aagagccgac ggattggtcg
     1081 attccgtata tcgacttctg ctactgggcg ccgctccagc agaataaagg attgctggag
     1141 ggggaattgg cggggattcc gattcctctc ttcaacggtg tttatcatga ttgtatcgta
     1201 acgccatggc agctaacgaa ggcggattcg gtcagcgata tcgatcagtt cttgtacgcg
     1261 ctttcgttcg gtggatgtcc gatggttggg aacccggatc gaaaggatgc cttcccggcg
     1321 ggcacgctcg agcgagccaa gatcctcgcc gatgcccatc ggcagacggg tttcgcggca
     1381 atcgagcggc acgattttct cagtcccgac ggctcgcagc gacgcacgac attcgcgaac
     1441 ggcttggctg tggaaattga tctccggaag cgacagtatc gttttgaagg tttcaagcgg
     1501 atgaagaaga agtggtccga cgtaccggga gcgggagcgt gagacggcgg aacttcgtcg
     1561 ggcttggacg tgttttgtag agtgacgtcc tctaccgttc gaagtgttga cgaatccgtt
     1621 ttgtgcctcg tatactacag aggacacttt cgagtttatc gcgcgcgtct ccgcgcccgt
     1681 tccatcagga gggaacatac atgaccgaag ccgtggaaac attagtgaac attaccgagc
     1741 cggcagcaaa taaaatcaag gaactgctcg agcaagaaga caactcggag tactgcttgc
     1801 ggcttcaggt cgtaggcggc ggctgctccg gatttcaata tcggcttgcg tttgacgacc
     1861 agatagccga cgacgacgaa atcgtcgatt acgacggcat caaggtcgtt gttgatccgt
     1921 tgagcgcacg ctacgttgaa ggtgcgcgaa tcgactatgt agaaacagaa atgggcgcgg
     1981 gattcaagat cgataacccg aatgtgcaga ccacatgcgg ttgtgggcat tcgttctcgg
     2041 cctaattgtc caactcatac agtcaccgcg aaagcgcgtc gtttgacgag atcgagcgac
     2101 gcgctttttc tgtgcgcgcc attcaccggt agcggtaaac ggcatatatc cgtatctttt
     2161 tctatcgcac tggatcggtc tatactccgg tttttcccat acctccacgg cagagcactc
     2221 cgaacgagcg aatatgcctg caacgaatca acacatacat ctgacgggta tctgcggaac
     2281 ggcgatggga gcgttggcgc tcatgctcaa ggagcgcggt taccatgtca ccggctcgga
     2341 tgcgcaagcg tatccgccaa tgagcaccgt tcttgccgaa aacggcatta cggtgatc
//
LOCUS       DSCC01000351            3443 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018556, whole genome shotgun sequence.
ACCESSION   DSCC01000351 DSCC01000000
VERSION     DSCC01000351.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018556"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            342..2189
                     /locus_tag="ENN56_04375"
     CDS             342..2189
                     /locus_tag="ENN56_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HDS74758.1"
                     /translation="MRSKLLLGAAVVGVALLLPELAFASEGAEGGHHLVTSIGISIIV
                     ATVLAFIANTFKQPLLLAYIIAGVVIGPHMGFHLIENRGDIETIAEIGLILLLFMIGL
                     EIDIKKLRESGSSLIVSGVTQFMICFALGIGFFFMIGFTLSNMQFSVLGFEIGGAGYD
                     LGYLAVCTAISSTTIVVKLLYEKFELDTLPGRITLGILVFQDLWAIVILGIQPNLADP
                     QILQILWSFGKGGILVVIALLISRYTLGKLFKSIAKAPELVLVASLGWCFFICGIASV
                     FDLSLEMGALIAGAAISTFPYNLDVIAKIVSIRDFFLTLFFVALGMQIPNPLDNPGIV
                     AIAALASVFVVLSRFIAVFPVLWGLRNGNRVSLLVPINLAQISEFALVIASLGFGAGH
                     ISQDTLSIVIFIFAITSITSTYMIKFSHPLQQMFNNLIQKIGIKDIEDAPTEGPVDAA
                     KDIAILGFFRVASSLVREIIDHEPQLAERIVVVDFNPNVHQKLQAMGIKAIYGDVANM
                     DTLHHAGIEHAKIVISTIPDTILKGTDNLRMIKSIQRICPHARIIVTAESASRAVKMY
                     QDGADYVFLPRILAAEHLIPVVNDLIERDDESLKETQMDILKAREEIIS"
     gene            2192..>3443
                     /locus_tag="ENN56_04380"
     CDS             2192..>3443
                     /locus_tag="ENN56_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HDS74759.1"
                     /translation="MLNSTKGHRFMRLPVVRASFIVIALTTMLIGCASPPVGIRPSGG
                     GDAVRIGLSEGASRVELAGPEDLRMTVGASTLVGRTFTLTLSGDEIRVTGSGNDARGR
                     KVLVSSNAPIRVGSKRYRGSIEVFPRAGGLTVVNVIAIEDYLCGVVPPEIGYLPVRDI
                     EAMKAQALAARTYVMAHYGRRIANGFDIYADTRDQVYGGVDAESDVGNRAVAETRNRV
                     ITYEGRLIEAYYHSTCGGSTVNIADVWDSPPVPYLTRVYDADDAGFHCAESPHFRWVE
                     VYSGQKARELISANLRNGVPDAPRSIGRLRDVAVETQTLSGRTASTRIVTENDAFSVR
                     KDKIRLVLRRPDGANPPLRSSYLRIFSDRTSEGFVSRVVVSGAGNGHGIGMCQWGAIG
                     MARKGYTAEQIVQHYYRGTQVTTYE"
BASE COUNT          704 a         1007 c          973 g          759 t
ORIGIN      
        1 atggtggtgc ggtcgcgggt gcccgtcgct gatgatcttt cggagtggaa gccgcgaacg
       61 accgccgagc gcgacctctt gcgcggctta tgcgattcgg tcgcgcgaac ctcgctgaca
      121 cagaacatca tcacgtaaaa cgcgtgtcgc gatagatcgc tgcaagattt ctcaatcgaa
      181 actaccataa gattactctt ccgccttgca tcacggcgtg gtacatgtat tgtagtgtac
      241 cgtcgatttt gcgacgcgag gttttccaac gcgtcgcaca atcgacactc gagcattccg
      301 tagggggaca catcggtaca accgatttgg gggatcgagc tatgcggtcg aaactactct
      361 tgggcgcggc ggtagttggt gtcgcgttac tcctgcccga gctggcgttc gcgtcagaag
      421 gcgcggaagg tggtcatcac cttgtcacga gtatcggcat cagcatcatc gtcgccaccg
      481 tcctcgcatt catcgcgaac acgttcaaac aaccgcttct tctcgcgtac atcatcgccg
      541 gtgtcgtgat cggcccgcac atgggcttcc atctgatcga gaaccgcggt gacatcgaaa
      601 cgatcgcgga aatcgggttg atccttctcc tcttcatgat cggcctcgag atcgatatca
      661 aaaagctgcg cgaatccggt agttcgctga ttgtatcggg tgtcacacag tttatgatct
      721 gtttcgcgct cggtatcggc tttttcttca tgatcggatt tacgctgagc aatatgcagt
      781 tcagcgttct cggcttcgag atcggtggcg cgggatacga tcttggctac ctcgcagtct
      841 gcaccgcgat cagcagtacc accatcgtcg tcaagctgtt atacgagaaa ttcgagcttg
      901 atacgctccc cggacgaatc acgctgggta ttctggtctt ccaggacttg tgggcgattg
      961 tcattctcgg tattcaaccc aacctcgccg atccgcagat ccttcagatt ctctggtcgt
     1021 ttggcaaggg tggcattctc gtcgttatcg cgttgctgat cagccggtac acactcggta
     1081 agcttttcaa gtccatcgcg aaagcgccgg aactggtcct cgtcgcatcg cttggatggt
     1141 gcttcttcat ctgcggtatc gcgtcggtgt tcgacctgtc gcttgagatg ggcgcgctga
     1201 tcgccggtgc cgcgatatcg acgttcccgt acaaccttga cgtgatcgcg aagattgtca
     1261 gtatccggga tttcttcctg acgctattct tcgtcgcgtt gggtatgcag atcccgaatc
     1321 cgcttgacaa tcccggtatt gtcgcgatcg cggctcttgc gtcggtgttc gttgtgcttt
     1381 cgcgcttcat tgctgtcttc ccggtcctct ggggccttcg caatgggaat cgcgtgagct
     1441 tgctggttcc aatcaatctc gcgcagatat cggaatttgc actcgtgatc gcgtctcttg
     1501 gattcggcgc gggccacatc tcgcaggata ccctctcgat tgtgatcttc atcttcgcga
     1561 ttacgtcaat tacctcgacg tacatgatca agttcagcca tccgctccag cagatgttca
     1621 acaacctgat tcagaagatc ggtatcaaag acatcgaaga cgcgccgacc gaggggccgg
     1681 ttgatgcggc taaggatatc gcgatactcg ggttcttccg cgtcgcaagc tcactcgtac
     1741 gtgagatcat cgatcacgaa cctcagcttg ccgagcgaat cgtcgtggtg gacttcaacc
     1801 cgaacgtgca tcagaagctc caagcgatgg gtatcaaggc gatctatggc gatgtagcga
     1861 acatggatac gctgcatcac gccggtatcg agcacgcaaa gatcgtcatc agcacgattc
     1921 ccgatacgat tctcaagggc accgacaacc tgcgtatgat caagtcaatc caacgcatct
     1981 gcccgcacgc acgaatcatc gtaacagccg agagcgcctc acgcgcggta aagatgtatc
     2041 aggacggcgc cgattacgtc tttctgccac gtattctcgc cgccgaacac ctgattcctg
     2101 tggtaaacga tcttatcgaa cgcgacgacg aatcgctcaa ggagacgcag atggatatcc
     2161 tcaaagcgcg cgaggagatc atcagttaat catgctcaat tcaacgaaag gacatcgttt
     2221 catgcgtctt cccgtggtgc gcgcatcgtt catcgtcata gcgctcacga ccatgctcat
     2281 cggctgcgcg tcgccaccgg taggcatcag gccgagtggc ggcggcgacg ccgtgcgaat
     2341 tggcttgtcg gaaggcgcaa gtcgtgttga gctcgccggt ccggaagacc tccgcatgac
     2401 cgtcggagcg tcgacactgg tcggacgcac attcacactc acgctctccg gtgacgaaat
     2461 ccgcgtgacc ggtagcggca atgacgcacg cggtcggaag gtgctcgtat cgtcgaacgc
     2521 accgatacga gtcgggagta aacgataccg cggttcgatc gaggtgtttc cacgcgccgg
     2581 tggtctgacg gttgtcaacg tcatcgcgat tgaagactac ctctgcggcg tcgttccacc
     2641 ggaaatcgga tacctccccg tgcgcgatat cgaagcgatg aaggctcagg cgctcgccgc
     2701 acgcacctac gtcatggcgc attacgggcg acgcatcgca aacggcttcg atatctacgc
     2761 cgatacacgc gatcaggtct acggcggcgt cgacgcggaa agcgacgttg ggaaccgagc
     2821 cgtcgcggaa acgcgcaatc gcgtcatcac gtacgaaggc cgcctgatcg aagcgtacta
     2881 tcacagcacg tgcggcggat cgacggtgaa tatcgccgac gtgtgggatt caccgccggt
     2941 accctacctc actcgcgtat acgacgcgga cgatgccgga ttccactgcg ccgagtcgcc
     3001 acacttccgc tgggtagagg tctactccgg acagaaggcg cgcgaactaa tctcggcgaa
     3061 cctgcgtaac ggcgtccctg atgcgccgcg cagcataggg cgcttgcgcg acgttgccgt
     3121 cgaaacgcag acactgagcg gacgcacggc gagcacgcgc atagtcaccg agaacgatgc
     3181 gttttccgtg cgtaaagaca aaatccggct cgtactgcga cgtcccgacg gtgcgaatcc
     3241 accacttcgc agctcatacc tccgtatctt ctctgaccgc acgtcggagg gtttcgtcag
     3301 tcgcgtcgtg gtgagtggcg cgggcaacgg gcacgggatc ggcatgtgtc agtggggcgc
     3361 gatcggcatg gcgcgcaagg gatacaccgc cgaacagatc gtgcagcact actaccgcgg
     3421 cacgcaggtg accacgtacg aac
//
LOCUS       DSCC01000352            1582 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052939, whole genome shotgun sequence.
ACCESSION   DSCC01000352 DSCC01000000
VERSION     DSCC01000352.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052939"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..181)
                     /locus_tag="ENN56_04385"
     CDS             complement(<1..181)
                     /locus_tag="ENN56_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74760.1"
                     /translation="MNKRLIARVATRIVLAFSLLLPVLVPAQAPVSRREADDYTFARR
                     LYDQGDYDLAERQLRD"
     gene            444..>1582
                     /locus_tag="ENN56_04390"
     CDS             444..>1582
                     /locus_tag="ENN56_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74761.1"
                     /translation="MDLDACKAAFASPPVEYRPAPFWFWNDHLDSTRLIEQFDSLIDA
                     GMGGAVLHARGGLDASEYLDESWFDAIGSIVKRAAERETKIWLYDELGWPSGSAGGRV
                     LRAHPDLRSMRLVMHDVSVFEIGALDTLPHDNIVAAYVVKRSDPEHGILHRTDRGNPL
                     PSAVTLLPDRIEYEPVEWPLKREAIVGQRVLLFECRRDGGTIDYLDPRGAEAFLESTH
                     EEYHRRFSEYFGTTITHSFMDEAGMLVGAATLPWTGNFAQRFRETRGYDLFARLPALF
                     FDVPGHEQVRYDFWNLATELFREGFGIPMHRWCEQHGIHYSGHYVFETTLKEATRQLG
                     STMPLYEYQGMPGIDVLGGDFYSRRFAPEDFGMYTVMLKQAASVV"
BASE COUNT          368 a          421 c          443 g          350 t
ORIGIN      
        1 aatcgcgaag ctgccgctcg gcgagatcgt aatcgccctg atcatagaga cgtcgtgcga
       61 acgtgtaatc gtccgcttcg cgtcggctta ccggtgcctg cgccggaacc aagaccggaa
      121 gaagcagact gaaagccaac acaatacggg ttgcgacacg agcgatgagt cgcttattca
      181 ttgcggagta ccggattcga gggatcatgg tgttggatgc agcgctaatt atcataggaa
      241 tgatacgcgc gtataccgac cgaggcaaac tatcgaacac ggcggtatag cccccaagtt
      301 tgcgcgtgat tacgttttac gaaatacacg tacgaacgac aatgttactg tgagtatcga
      361 acgcgcttac gaacagaact gttccccgtt gatacggatt gtgactgtac tgtaagatcg
      421 ataacgatgg aaggaaatct gacatggacc tcgacgcgtg taaagcggca ttcgcctcgc
      481 cgccggtgga ataccgtccg gcgccgttct ggttctggaa tgatcacctt gattcgacgc
      541 ggctcatcga gcagttcgac tcgcttatcg atgcgggcat gggaggcgcc gttcttcatg
      601 ctcgcggcgg attagacgca agcgagtacc ttgacgaatc atggttcgac gcgatcggta
      661 gtattgtaaa acgagcagcc gaacgagaaa cgaaaatctg gttatacgac gaacttggct
      721 ggccttccgg tagtgcaggt gggcgtgttc ttcgtgcgca tccggatctc cgttccatgc
      781 gtctcgttat gcacgatgtg tccgttttcg agatcggtgc actcgacacg ttaccacacg
      841 ataacattgt cgccgcgtac gtcgtaaaac ggtccgatcc tgagcatggg atcttgcatc
      901 ggacggaccg tggcaatcca cttcccagtg ccgtaacact actgcccgat agaatcgagt
      961 atgaacctgt cgaatggcct ttgaaacgag aagcaatcgt aggacagcgt gtgctgttgt
     1021 ttgagtgtcg gcgcgacggt gggacgatag actacctcga cccgcgtggt gcggaagcat
     1081 ttctcgagtc cacccacgaa gagtaccatc gccgtttctc ggaatacttc ggcacaacca
     1141 tcacacactc gtttatggac gaagccggta tgttggtcgg cgcggcaacg ttgccttgga
     1201 ccggcaactt cgcacagcgc ttccgcgaaa cacgaggcta cgatctcttc gcgcggctac
     1261 cggcactgtt cttcgacgta cccggtcacg agcaagtgcg ctacgacttc tggaaccttg
     1321 caacagaact cttccgagaa ggtttcggga ttccgatgca ccgctggtgc gaacagcacg
     1381 gtatccacta ctcaggccac tatgtatttg agacgacact caaagaggcg acgcgccaac
     1441 tcggtagcac aatgccgtta tacgagtatc aaggaatgcc gggaatcgac gttctcggtg
     1501 gagatttcta tagccgtcga ttcgcgccgg aagatttcgg tatgtacacc gtcatgctga
     1561 agcaggccgc atcagtcgtg ca
//
LOCUS       DSCC01000353            3462 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018413, whole genome shotgun sequence.
ACCESSION   DSCC01000353 DSCC01000000
VERSION     DSCC01000353.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018413"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            184..915
                     /gene="truA"
                     /locus_tag="ENN56_04395"
     CDS             184..915
                     /gene="truA"
                     /locus_tag="ENN56_04395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:PRK00021.2-1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDS74762.1"
                     /translation="MSRRLRLDIEYDGTDFAGWQIQPNERTIQGEIAEALSTILRQPV
                     SITGAGRTDAGVHARGQVAHVDVPDDAPEPYRILAGVNALTGRDIAVYAARYVSDDFH
                     ARFSARSRTYAYRISLRPTAIDRRLTWQVPGELDVVAMQQAAERLVGRYPATTWCAAG
                     AEDRDAIVDVRSAKVSVVGPQLVFAIRADRFVMHMVRTIVGTLVEIGHGKRTAQSIPD
                     LIDARDRSSAGETAPARGLCLEHVE"
     gene            806..2152
                     /locus_tag="ENN56_04400"
     CDS             806..2152
                     /locus_tag="ENN56_04400"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00089.24,HMM:PF13180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HDS74763.1"
                     /translation="MGSVRRRVFPISLTRAIDPPREKPHPHEDCVSNTWNKIPMQTPQ
                     TDWQPTRTRHTRFVMGLFVGVVASVGGMFVADRIATNPDTAEQAVPTLPEPPASFVSS
                     EDVDATRRTAIVQAVESVSPAVVTISVTQLRRERVVTPFNDPMWQFFFPDLGREFIRK
                     VNSMGSGFIVSEDGLIVTNEHVIRGASEIVVNLPDGRSFNGEVFGEPDAQTDLAIIKI
                     EGDDLPVAPLGRSDDLMIGEWAIAIGNPFGYLIRDAHPTVTVGVISALNRDFAASTAN
                     DRSYRDMIQTDASINPGNSGGPLINSEGYVIGVNTFIFSQSGGSQGIGFAIPVRRVRM
                     IIDDILRYGEVRHNFWTGIHIQDVTRWIAESLSLPSDRGAIISDVDAGSPGEKAGLRR
                     GDVIIAVGRKRVASARDVREEFVGAVVGQQIPLTVIRGGNEMTVTMTLEELPSERR"
     gene            2154..2792
                     /locus_tag="ENN56_04405"
     CDS             2154..2792
                     /locus_tag="ENN56_04405"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HDS74764.1"
                     /translation="MAKAGIPFIVGAIVVAAIFFAVWCGLRWEVFRALGVLALIFAAF
                     SAFFFRDPERTIPQEPKLVVAPADGKIIEIVTEDNKFVGPNAKRVTIFLSVFDVHINR
                     IPISGTVTALEYNPGKFLMAWHEKASADNEQTHIAIESPYGTIAFKQIAGWVARRIVY
                     EIKPGDTVNTGDRFGLIRFGSRADIIFPPDAEIRVQPGERARGGETVIGVLR"
     gene            2789..>3462
                     /gene="pssA"
                     /locus_tag="ENN56_04410"
     CDS             2789..>3462
                     /gene="pssA"
                     /locus_tag="ENN56_04410"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDS74765.1"
                     /translation="MSIITQSIPSVFTLANLFMGMLSMINAMEGTEAGFTRAAWFIVA
                     AAIFDTLDGKASRVLNTASKFGVELDSIVDACSFGIAPALLAYQYILTYLPAWSAFAF
                     PVAFVYAACVTLRLARFNVQLVGFSKTSFTGMPSPMGAGLVATFVAFANTDLMANIAA
                     EHVFPYIVIIAAALMVSSVKYDTMPKLSWSGGYNRFKLVTLLTAIGLAVFFPAEVFFP
                     TGVLYMS"
BASE COUNT          670 a          996 c         1073 g          723 t
ORIGIN      
        1 attcgaacgc tcgtttgacc gcgccgacat cctcgccgag gcgatggaag cgcgcggata
       61 ttcgtcgggt accgagcgga ctcattaccg acgacttggc ttcaccgcgg tcgatctcgt
      121 cgcggtgggt gtcgttgcag cgtggctcgg cgtcgcgatc gtcgtcgagc gggcgtttca
      181 cgtatgagca gacggctcag gctcgacatc gagtacgacg gtaccgactt cgccggatgg
      241 cagatacaac cgaacgagcg aacgattcaa ggcgagatcg ccgaagcgct ttcgacgatc
      301 ctgcggcagc cggtatcgat aaccggcgct gggcgcacgg acgccggagt ccacgcgcgc
      361 ggccaggttg cgcatgtcga cgtaccggac gatgcgccgg agccgtatcg gatactcgcg
      421 ggtgtgaatg cgctgaccgg tcgtgatatc gccgtgtacg ccgcgcgata cgtttcggac
      481 gactttcacg cccggttttc ggcacgttcg cgcacatatg cgtatcgcat ctcgctacga
      541 ccgaccgcga tagatcgtcg cctcacgtgg caggtgcctg gagagcttga cgtcgtcgcg
      601 atgcagcagg cggccgaacg actggtcgga cggtatccgg cgacgacgtg gtgcgcggcg
      661 ggagcagagg accgagacgc gattgtcgac gtccgcagtg cgaaggtcag cgtcgtcgga
      721 ccgcagctgg tgttcgcgat acgtgcggat cgcttcgtga tgcacatggt gcggacgatt
      781 gtcggcacac tggtagagat cgggcatggg aagcgtacgg cgcagagtat tcccgatctc
      841 attgacgcgc gcgatcgatc ctccgcggga gaaaccgcac ccgcacgagg attgtgtctc
      901 gaacacgtgg aataagatac cgatgcagac cccgcagact gattggcaac cgacgcgaac
      961 ccgacacacc cgtttcgtga tgggtctgtt cgtcggcgtt gttgcgagtg tcggtggcat
     1021 gttcgtcgcc gaccgcatcg ccaccaaccc ggataccgcc gaacaagcgg ttccgacctt
     1081 accggaaccg cccgcctcgt ttgtctcgtc ggaagacgtc gacgcgaccc gtcgaaccgc
     1141 catcgtccag gcggtagaga gcgtctcgcc ggcggttgtc acgatcagcg tcacacagct
     1201 ccgtcgcgag cgcgtggtaa cgccgttcaa cgatccgatg tggcagtttt tcttccccga
     1261 tctcggccgc gaattcattc ggaaagtgaa ttcgatgggg agcggattca tcgtatccga
     1321 ggacgggctg atcgtcacga acgagcatgt gattcgcggc gcgtcggaga tcgtcgtgaa
     1381 cctgccggac gggcggtcgt tcaacggcga ggtcttcggt gagcccgacg cgcagaccga
     1441 tctcgcgatt atcaagatcg aaggcgacga cctgccggtg gcgccgcttg gacgcagcga
     1501 cgatctcatg atcggcgaat gggcgatcgc cattggtaac ccgttcggct acctcattcg
     1561 cgatgcgcat ccgacggtga cggtgggtgt catcagcgcg ttgaatcgcg atttcgccgc
     1621 gtcgacggcg aacgaccggt cctaccgcga tatgattcag accgacgcgt ccatcaaccc
     1681 gggaaacagt ggcggaccac tgataaatag cgaaggctac gtcatcggag tgaatacgtt
     1741 catatttagt cagagcggcg gttcgcaggg catcgggttc gcgataccgg ttcgccgcgt
     1801 gcgaatgatt attgatgaca tcttgcgtta cggcgaagtt cgacacaact tctggactgg
     1861 cattcacatt caagacgtca cccggtggat tgccgagagc ctctcgttgc catcggatcg
     1921 cggcgcgatt atttccgacg tcgacgcggg aagtccgggc gagaaggccg gattgcgtcg
     1981 cggtgacgtg atcatcgccg tcggcaggaa gcgcgtcgca agcgcgcgcg atgtacgcga
     2041 ggaatttgtc ggcgcggtag tcgggcagca gataccccta acggtgatac gcggtggcaa
     2101 cgaaatgaca gtgacgatga cgctcgaaga gcttccgtcg gagaggagat gacgtggcca
     2161 aggccggtat accgttcatc gtaggcgcga ttgttgttgc agctatcttc ttcgcggtct
     2221 ggtgtgggct gcgttgggag gtcttccgcg cactcggtgt tctggcgctc attttcgctg
     2281 cattcagcgc gttcttcttc cgtgaccccg agcgcacaat tccacaggaa ccgaagctcg
     2341 tcgtggcgcc cgccgacggc aaaatcatcg aaatcgttac cgaagacaac aagttcgtcg
     2401 gaccgaatgc gaaacgggta acgatattcc tctccgtatt cgacgtgcat ataaaccgaa
     2461 ttccgatcag cggcaccgtg accgccctcg agtacaaccc cggcaagttt ctcatggcgt
     2521 ggcacgaaaa ggcgtcggcg gataacgagc agacacatat cgctatcgaa tcaccctacg
     2581 gcaccatcgc attcaagcaa attgccggat gggtggctcg gcgcatcgtc tatgaaatca
     2641 aaccaggtga taccgtgaat accggcgatc gattcggtct gattcgattc ggtagccgcg
     2701 ccgatatcat cttcccaccg gacgccgaga tacgcgtaca gccgggcgaa cgtgcgcgtg
     2761 gcggcgaaac ggtgattgga gtactccgat gagtattatc acccagtcga ttccaagcgt
     2821 attcacgctc gcgaacctgt tcatgggtat gctttccatg atcaacgcca tggaaggaac
     2881 cgaggcgggt ttcacgcgag ccgcgtggtt tatcgtggcg gctgcgatct ttgacacact
     2941 cgacgggaag gcttcccgcg tcttgaacac cgccagcaag ttcggcgtcg aacttgattc
     3001 gattgtcgac gcgtgttcat tcggtatcgc gccggctttg ctcgcgtacc agtatatatt
     3061 gacgtacctc cccgcatgga gcgcgtttgc gtttcccgtg gcgtttgtct atgcggcgtg
     3121 tgttacgttg cgcctcgcgc ggttcaacgt ccagttggtt ggattctcga agacgtcttt
     3181 caccggcatg ccgagtccga tgggcgccgg actcgtcgca acgttcgtcg cgtttgcaaa
     3241 taccgatctc atggcgaata tcgccgccga gcacgtgttc ccgtatatcg ttatcatcgc
     3301 cgctgcgctc atggtgagtt cggtgaaata cgacaccatg ccgaagcttt cgtggagcgg
     3361 tgggtataac agattcaagc tcgtcaccct cttgacggct attggcctcg cagtgttctt
     3421 ccccgcggaa gtgttcttcc caacgggagt tttgtacatg tc
//
LOCUS       DSCC01000354            3447 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018520, whole genome shotgun sequence.
ACCESSION   DSCC01000354 DSCC01000000
VERSION     DSCC01000354.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018520"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(4..2457)
                     /locus_tag="ENN56_04415"
     CDS             complement(4..2457)
                     /locus_tag="ENN56_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74766.1"
                     /translation="MPITIETGVRGGANTPSRECRADCVSVSANDYHVVVCGSEVIVE
                     TSATTGWQPIVRSAPFGSASGGNEDDETYVSALGGKLIRTYQPDADTAVLEFAATHGT
                     HHVVTRMFISTGEPLLRIVAVDGILGEADVKRFGVAWEFVSGPKDGSRPDFIFTPCLA
                     PKPGQVIGQHMFRSPAAIIQHHEVAFALVPDIDEIRAYQPLRMYLDVEPPAPGRPTIL
                     STGVAAYRLDGHVYFTTADVEPVHLKDTQLRFGCELHLDAVAPPSRAHQAIVRHHWKQ
                     HGRTWLRRDVLPQTVPFKRYFDYGYEYAESDLWRETTVGEKTVGAMVIGREYERDVWF
                     QGWFNQLRTAYGYFLWARRLGDSERLNRARATRDLVLSAPQVEEGPTAGMFPTIAIMP
                     ESDGEPVRWVESTLQGGGPGLFHPLDMSWTATWLVRWHAELEPNEETITFCRRYADAL
                     LRLQSNDGSWPDYVDAEGNVLTEFTDVESVTRCDSGYIKKMHRDWWDTKRLAASAESA
                     ANVLFLAEFSRHVDDAKRYLKAALAGAAFLEQQVIPEHRWFDWETFFSCSPKPIGFYD
                     ERTMQHPQNAMSMFFATEAFRVLYEITGTATLGEQAVSLMDYVCLYQQVWSPPFLTFH
                     GFGGFGAMNTDGEWNDARQAVFADGLARHYLESGRQEYLERAVAATRASFTCTFIPEN
                     ARVAPKIFDRTPTGHADENYAHLGIDIPAGATGFDWGTGSGLTSAALLLERFGDLWID
                     VIEGWALGIDALTVIDCECGAGTWKLTINAPFSHTDTITLKGSPCEYESGVRLVINDL
                     EPITVTDDDLVHGKRVRIR"
     gene            2667..>3447
                     /locus_tag="ENN56_04420"
     CDS             2667..>3447
                     /locus_tag="ENN56_04420"
                     /inference="COORDINATES: protein motif:HMM:PF04151.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74767.1"
                     /translation="MKPSITPSRTIRFVFLAAASILALSLSACILSDSDSEPEMGNGG
                     TISVGQTKTGSITSDKYAWRYTFTLTETKMVVITMEAVNGEIDPFLDLRDSNDISIAT
                     DDDGYWDLEQDWQAEDLDAAIRTVLVPGTYTIVAMSFENAYIEQMQMHEDVEISVLET
                     GDFALTLRTDPVGDGGTIAIGGNASNTIDDVTYGWLYTLTVPVATSLSIDMEMTSGTL
                     DAYLEVYNSSNTLVTANDDGGTGSNSRIQYTFAAGTYTVFAT"
BASE COUNT          717 a          988 c         1012 g          730 t
ORIGIN      
        1 ccgttaccgt atacgaactc gtttcccatg cacgaggtca tcgtcggtaa cggtaatcgg
       61 ttcgagatcg ttgatgacga ggcggacacc gctttcgtac tcgcagggtg aacccttgag
      121 cgtaatcgta tccgtgtggg agaacggggc gttaatcgtc agcttccacg tgcccgcgcc
      181 gcattcgcag tcgatgaccg tgagcgcgtc gattccaagc gcccatcctt cgatgacgtc
      241 tatccacagg tcgccgaatc gctcgagaag cagcgctgcg gaggtcagtc cgctgccggt
      301 tccccagtcg aatccggtcg cgccggcggg aatgtcaatg ccgagatgtg cgtagttctc
      361 atcagcgtga ccggttggcg tccggtcgaa gatttttggc gcaacacgcg cgttctcggg
      421 gatgaatgtg cacgtaaacg atgctctcgt cgcggcaaca gcgcgttcca gatactcttg
      481 gcgaccactt tcgaggtagt ggcgcgcgag cccgtcggcg aataccgcct gacgagcgtc
      541 gttccattca ccgtcggtat tcatcgcacc gaaaccaccg aacccgtgga atgtgaggaa
      601 tggtggtgac cagacctgtt ggtagagaca cacgtaatcc atgagcgaca ccgcctgttc
      661 tccaagagtc gccgtgccgg tgatttcgta gagaacgcgg aacgcttcag tggcgaagaa
      721 catactcatg gcgttttgcg gatgttgcat tgtgcgttcg tcgtagaaac cgattggctt
      781 cggcgaacac gagaagaacg tctcccagtc gaaccaacgg tgttccggaa taacctgctg
      841 ctcgagaaat gccgcaccgg caagcgcggc cttgagatac cgtttcgcat catcgacgtg
      901 gcggctgaac tcggcgagaa acaacacatt cgccgcgctc tcggcactcg ccgcgaggcg
      961 tttcgtatcc caccagtcgc gatgcatttt cttgatatat ccagaatcgc atcgcgtcac
     1021 ggattccacg tcggtaaact cggttaggac gttcccctcc gcgtcgacat aatcgggcca
     1081 gctgccgtca ttcgactgaa gccgtagcag cgcgtccgcg tatctcctgc aaaacgtaat
     1141 ggtttcctcg ttcggctcca gttctgcgtg ccaacggacg agccacgtcg ccgtccacga
     1201 catgtcgagc ggatggaaca agcccggtcc gccgccctgc aatgtgcttt ccacccagcg
     1261 caccggttca ccgtcggatt ccggcatgat ggcaatcgtg ggaaacatac ccgcggtcgg
     1321 gccttcctcg acctgtggcg cgctgagcac gaggtcacgt gtggcgcgtg cgcgattgag
     1381 tcgctccgaa tctccgaggc gtcgtgccca gagaaagtac ccgtacgccg tgcgaagctg
     1441 attgaaccag ccctgaaacc atacatcgcg ctcgtactcg cgcccgatga ccatcgcgcc
     1501 gacggttttc tcgccgaccg ttgtttctcg ccagaggtcc gactcagcgt attcgtaacc
     1561 gtaatcgaag tagcgtttga atggcaccgt ctgggggagc acgtcgcgac ggagccatgt
     1621 gcgcccgtgc tgtttccaat gatgccgtac aatcgcctgg tgcgcgcgcg acggcggcgc
     1681 aacggcgtcg aggtgaagct cgcaaccaaa ccggagttgc gtgtctttga gatggaccgg
     1741 ttcgacatcc gccgtggtaa agtacacgtg tccgtcgagg cgatatgctg cgacaccggt
     1801 gctgaggata gtgggacgac ccggcgccgg tggttcgacg tcgagataca tacggagcgg
     1861 ttggtacgca cggatttcgt cgatatccgg gaccaacgcg aaggcgacct cgtggtgctg
     1921 gatgatcgcc gcagggctgc gaaacatgtg ctgcccaatg acctgtccgg gttttggcgc
     1981 gagacacggc gtgaagatga aatcggggcg gcttccgtcc ttcgggccgc tgacaaactc
     2041 ccatgcgaca ccgaatcgct tcacgtccgc ctcgccgaga atgccgtcga ctgcaacaat
     2101 ccggaggagc ggttcgcccg tcgaaatgaa cattcgcgtg acgacgtggt gcgtaccgtg
     2161 tgtcgccgcg aattcgagaa cggcggtgtc ggcgtctggc tgatacgtcc gaatgagctt
     2221 cccaccgagc gcggagacgt acgtttcgtc gtcttcgttg ccaccggatg ccgaaccgaa
     2281 cggcgcgctg cggacgatag gttgccatcc ggtggtcgcc gacgtttcga cgataacttc
     2341 agagccacac acgactacat ggtagtcatt tgcactgacg gaaacgcagt cggcgcggca
     2401 ctcacgcgag ggcgtattgg caccgccgcg cacacccgtt tcgatcgtga tcggcattca
     2461 aggtccttcc accacaagga tcgtttacga agatcgagtc aatcgataaa agtataccgc
     2521 ttacgctgaa atggggaaac gttctccgtg gaactccgta cgggatctgc gtcgttgtta
     2581 gatgaggcgt tgacagattc tgtctacacg atatcttagg taatgataac cgcgtacaat
     2641 gtgtccagtt atttcggagg tcacgagtga agccatcgat tacgccttcg cgcaccatcc
     2701 ggttcgtgtt tctcgctgcc gcatccattc tcgcactctc cctgtcagcc tgtattctgt
     2761 ctgattcgga tagcgaaccg gagatgggaa atggtggcac gataagcgtc ggacagacga
     2821 aaaccggttc gatcacgagt gacaaatatg cgtggcgata tacgtttacg ctgaccgaga
     2881 cgaagatggt cgtcatcacc atggaagcgg tcaatggtga gatagaccca ttcttggacc
     2941 ttcgcgactc gaatgacatc tccatcgcaa ctgatgacga cgggtattgg gatctggagc
     3001 aagactggca agccgaagat cttgatgcgg cgatccgcac ggtattggtt ccgggcacgt
     3061 ataccattgt cgcgatgtcg tttgagaatg cctacatcga gcagatgcag atgcacgagg
     3121 acgtggagat cagcgttctt gaaacgggtg actttgcgtt gacgctccgc accgaccccg
     3181 tcggtgacgg tggaacgatc gcgattggcg gaaacgcgtc gaacactatt gacgacgtga
     3241 cctacggttg gttgtatacg ctgacggttc ccgttgcaac ttcattgtcg atcgatatgg
     3301 aaatgacctc gggtacgctc gacgcgtatc tggaagttta caactcgtcg aacaccctcg
     3361 tcacggcaaa cgacgacggc ggaaccggct cgaattcacg aattcagtac accttcgccg
     3421 ccggtacgta cacggtcttt gcaacac
//
LOCUS       DSCC01000355            6641 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1007478, whole genome shotgun sequence.
ACCESSION   DSCC01000355 DSCC01000000
VERSION     DSCC01000355.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1007478"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..863)
                     /locus_tag="ENN56_04425"
     CDS             complement(<1..863)
                     /locus_tag="ENN56_04425"
                     /inference="COORDINATES: protein motif:HMM:PF01663.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDS74768.1"
                     /translation="MSMNLIVVFGDEMRGQAMSCAGDPNVRTPNMDRMAAEGRMFSHA
                     FSNSPVCTPARGMMLTGLFPNTHGAIVNDLPVHTDVPSIAWTLQNCGYRCGYVGKWHL
                     GGIPRSRRIPPGPDRLGFDAFWASWNCHHDYFEPKYFVNDDTEPTVADGYEPTVQTDL
                     ALDFMRDHFAEHEGNPFCLFLSWGPPHSPYTPWPPASNEMYDPDELILRPNCPDTPDH
                     RRDLAGYYAHITALDAELGRILDFVDESGHTEDTLIVFTSDHGSMLGSQGHYHKQQPW
                     GESLSVPLIMRG"
     gene            complement(906..1358)
                     /locus_tag="ENN56_04430"
     CDS             complement(906..1358)
                     /locus_tag="ENN56_04430"
                     /inference="COORDINATES: protein motif:HMM:PF01966.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDS74769.1"
                     /translation="MENRIRAMVSSVSKTCAPYCTCGDLGIHGFPHLVQVATTAGLIA
                     TELGEDIQSAVVGGFLHDCARTNDAGGRGHAIDSAILGAWLLGRFFPELDANRKRICE
                     AISWHADGKTTNDMLIACIWDADRLDLKRLHRVIDPRFLSTDVAKRMI"
     gene            1565..2422
                     /locus_tag="ENN56_04435"
     CDS             1565..2422
                     /locus_tag="ENN56_04435"
                     /inference="COORDINATES: protein motif:HMM:PF00582.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDS74770.1"
                     /translation="MICKILAALDNSDCGRSAGKYAAYLGAELNAGVEALTVVDIRPL
                     EGPILRDFTAHLGLEPFETYTQALRDVLQQRADDVLELFRQDAAAQSLDPERVSAGSE
                     LGIVAETICRRANSADLVVIGQFGESPAASGGLLGSTSEQVVRRTNRPVIVCPPMFRP
                     ITKALVAYDATPKAEDALHVAADFAAQLGIALTVLVVQDGEQVDEERATRIREQAERY
                     LSNAHLRAEFSVVEGNPEEVIIDSADKGDHDLVAMGAFGHGRIRQLFVGSVTAHVMRS
                     SKVPVLLSR"
     gene            2438..3013
                     /locus_tag="ENN56_04440"
     CDS             2438..3013
                     /locus_tag="ENN56_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74771.1"
                     /translation="MNQREINSNYKSAVALARDIARESLFGHIRGAPNPDDAVEDLAE
                     SIVGTESVVQLVVSIPFDGEAYRPVLLAAADEVGRLFFPDVHKRFISSLDAVSDQALV
                     CDDAYSEYDEPLAENRIVERLPNELREYFDALVEHHGVDQLSSRLWSDADADVLVARA
                     SVSVVTSQTLLSRRVPISIWTVTLPMERFAT"
     gene            2974..4404
                     /locus_tag="ENN56_04445"
     CDS             2974..4404
                     /locus_tag="ENN56_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74772.1"
                     /translation="MDRDLADGEIRDVTRLVAAGITLAFPWGFFSRQTKKRVTVATEY
                     LLRYEIRRTPEEVLGEGMMSLVALGLGPAIRRCGGSVNRLLAHAFPDEVRPWMSSHVP
                     TGYWEDENHRRNAVRWLVEECIGVQPDAIAEAIHAGRITKKDFADAGLTWLVKEVYRW
                     SVADALAEAYPTLEPWERLRRLPTEFWRVDDGGATAARAIRWALDRGEVGVDELRHQK
                     ASRTIAAALRPWKLVSAFSVGFDGDAFRCLDTLFPNTFRPWEVANVPRDDWKDAKLRQ
                     TALFWLLDRLGIDPSEIPAAIAQGRLTPASFTDNGLDGLLRVTGSVWRVVCDVFPDQF
                     ARWELGTVPRSHWRSRANVREAVLWAMKRLGISEQSLGSAIRDGRMTTNALVNLGLGS
                     LIVGVFRGDVTAMCKVADVLPSESYVPLSRYYRQSASGQSADRGASRLDAARRRAQSD
                     LMNESELDRHLSVSRSIREQRRRRTD"
     gene            complement(4410..5654)
                     /locus_tag="ENN56_04450"
     CDS             complement(4410..5654)
                     /locus_tag="ENN56_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74773.1"
                     /translation="MSFTQSDRYVLRELAKRYVDVCARDVNAECRDLWRAHNSLIATR
                     PLIYTRAFAWCEMPDAELTVEDPFFRGYEDWLRRQLFWASLNDDSVFEPWIPARAAVI
                     TPAGGVWGVSPTAIDSRTPRGAKKWIPPIATEDDFTKLIPPHHVIDEERTAERLARTI
                     EVFGDILPVFADRAPEYRMWNGDISTQIAYLRGLDQIMLDMIDRPEWLHRLLTFMRDG
                     IIRTHEQAEEVGDWSLINHQNQSMPYATELPDPAPSSTTPTGESVKRKHLWYYCASQE
                     TTGVGPTMFNEFMLEYQIPIMRPFGLVAYGCCEDLTHKIEAIRAIPNLRRIAVAPSAN
                     VAASAERIGTEYVISYRPNPAMIATGFDADYIRDSLRKDLSACRGLHVDITLKDVETV
                     EGDPNRVSDWVRIAREVIDEVW"
     gene            complement(5662..>6641)
                     /locus_tag="ENN56_04455"
     CDS             complement(5662..>6641)
                     /locus_tag="ENN56_04455"
                     /inference="COORDINATES: protein
                     motif:HMM:PF12904.5,HMM:PF13204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HDS74774.1"
                     /translation="VGLMPCIVGMWGYYLPEIGVERIKRYWRYIVARYGAYPVCWCIA
                     GEGTMAWYLSENREAEAKQQLEGWTEVARYVRSIDGWRNPITIHPTQYGRKQVTDPGV
                     LDFEMLQTGHSSRLSLVNNVETVRTAYAEEPTMPTYVSEVNYEGILGRSWEDVQRASF
                     WQPVLSGAFGHTYGANGIWQMSRTDDPYGPSPHGRSWGEATWQEAYQLPGSRQVGYAR
                     RLLDTIPWWELEPHNEWVENPWDGSDPDRNICAGIPRKLRVVYMPHTWNVSKVLGLES
                     DVAYTAFWFDPVNGERTDIGDATPDPDGSWTAPHPVVVHDWVLVLRAKE"
BASE COUNT         1326 a         1913 c         1999 g         1403 t
ORIGIN      
        1 ccccgcatga tgagcgggac gctgagcgat tctccccacg gttgttgttt gtgataatgt
       61 ccttgcgaac cgagcatcga accgtgatca ctcgtaaaca cgatgagtgt gtcttccgtg
      121 tgtccggatt cgtcgacgaa atcgaggatt cggccaagct cggcgtcgag cgcggtgatg
      181 tgcgcgtagt aaccggcgag atctcgacgg tggtcgggag tatcggggca gttcgggcgg
      241 aggatgagtt cgtcggggtc atacatctcg ttcgatgccg gtggccacgg cgtatacggg
      301 ctatgtggcg gtccccacga aaggaatagg cagaacggat tgccttcgtg ttcagcgaaa
      361 tggtcgcgca taaagtcgag cgcgagatcg gtctgtaccg taggttcgta cccgtcggcg
      421 actgtcggtt ccgtatcgtc gttcacgaag tatttcggct cgaaataatc gtggtggcag
      481 ttccacgaag cccaaaatgc gtcgaagccg aggcgatccg gtcccggtgg gattctccgg
      541 ctgcgtggga taccaccaag atgccatttc ccgacgtatc cgcaacggta tccacagttc
      601 tgcagcgtcc atgcgatgct cggaacatcc gtgtgaactg gaagatcgtt tacaatcgca
      661 ccgtgggtat ttggaaaaag cccggtgagc atcataccac gagcgggggt gcaaacgggg
      721 ctgttggaaa acgcgtgaga gaacatccga ccttcagcgg ccatgcgatc catgttggga
      781 gttctgacgt ttgggtcgcc cgcgcagctc atggcttgac cgcgcatttc gtcgccgaaa
      841 accacaatga ggttcatcga catatcgacc tctttcgaga tacattctca caccgaccaa
      901 cgacgtcaga tcatccgttt cgcgacgtcg gtcgagagaa aacgcggatc gataacacgg
      961 tgaagtcgct tgagatcgag ccgatcagcg tcccagatgc acgcgataag catatcattg
     1021 gtagtctttc cgtccgcgtg ccaactaatc gcctcgcata ttcgcttccg attcgcgtcc
     1081 agttcgggga aaaaccgccc aagcaaccac gccccaagaa tcgccgaatc aatcgcgtga
     1141 cctcgtccgc cagcgtcgtt tgttcgcgca caatcgtgca gaaaaccgcc aaccacagcc
     1201 gattggatgt cttctcccag ctcggtagcg attaatcccg ccgttgtcgc gacctgtacg
     1261 aggtgcggaa aaccgtgaat accgagatcg ccacacgtgc aatagggtgc gcacgtcttg
     1321 ctgactgaag ataccatcgc tcggatgcga ttttccataa cctactccct actaagtagt
     1381 tagacacgtc gatcggcata ccgtccgtcg gcgatctcac tacatctata caggaaatac
     1441 cgagtatccg caaccaacaa gactttgggt catgcgacgc acgcagcctt gccaaaagct
     1501 gtgaaaacgc cgatttttgt cggtacgatc acgcctgaaa cgacattttc gggaggaggc
     1561 gctcatgata tgcaagattc ttgccgcgct ggataactca gattgcggtc gatctgccgg
     1621 gaaatacgca gcgtacctcg gcgctgagtt gaatgcggga gtcgaagcgc ttaccgtggt
     1681 tgatatccgg ccgcttgaag gtcccattct tcgggatttc accgcgcatc tcggcctgga
     1741 accgttcgag acgtataccc aggcacttcg cgacgtgctc caacaacgcg cagacgacgt
     1801 gctcgagctc tttcgacaag acgccgccgc acaatctctc gatcccgaac gcgtttcggc
     1861 gggctcggag ttgggtatcg tcgccgaaac gatctgccgg cgagcgaaca gcgcggactt
     1921 ggtcgtgatc gggcagttcg gtgaatcgcc ggcggcatcc ggtggtttgc tcgggtcgac
     1981 gagcgaacaa gtggttcggc gcacgaatcg tccagtgatt gtgtgtccgc cgatgttccg
     2041 gccaattacg aaggcgctcg ttgcgtacga cgccacgccg aaagcggaag acgcgctcca
     2101 cgtcgctgca gatttcgcgg cgcaactcgg aattgcgctg acggtgctcg ttgtgcaaga
     2161 tggcgaacaa gtcgacgaag aacgcgcgac ccgtatccgc gagcaagctg aacggtacct
     2221 ctcgaacgcg catctgcgag ctgagttctc tgttgtcgaa gggaaccccg aggaagtcat
     2281 catcgattcc gctgataaag gagatcacga tcttgtcgcg atgggtgctt tcgggcacgg
     2341 gagaattcgg cagctcttcg ttggctcggt aaccgcgcac gtcatgcgat cgagtaaagt
     2401 tcccgtgcta ctgtctcgat aggaacggga agtctcggtg aatcagcgag agatcaacag
     2461 caattacaaa agcgccgttg cactcgcacg tgacatcgca cgcgaatcgt tgttcggaca
     2521 cattcgcggg gcgccgaacc ccgacgatgc tgtggaagat cttgcggaat ccattgtcgg
     2581 caccgaaagt gtcgtgcagc ttgtcgtctc gattcccttt gatggagaag cgtaccggcc
     2641 ggtcctgctc gccgctgctg acgaagtcgg acggctcttc ttccccgatg tccacaagcg
     2701 ttttatcagc agcctcgacg cagtttcgga ccaggcgctc gtctgtgatg atgcttactc
     2761 cgagtacgac gagccacttg cagagaatag gatagtcgag cgtctcccaa atgagctacg
     2821 cgagtatttc gacgcattgg tggagcatca cggcgttgat cagctttctt cgcggctttg
     2881 gtccgatgcc gatgcagatg tgctcgtggc tcgagcgagc gtctccgtcg tcacttccca
     2941 gacgctgtta tcccgtcgcg ttccgatatc gatatggacc gtgaccttgc cgatggagag
     3001 attcgcgacg taacacgact cgttgcggca gggattacgc tggcatttcc gtggggattc
     3061 ttctcacgac aaacaaaaaa acgcgttaca gttgcgacgg agtatctcct ccggtatgaa
     3121 attcgccgta cgcccgagga ggtacttggt gaagggatga tgtcgctggt ggcgctcggg
     3181 ctcggtcctg cgattcggcg gtgcggcgga tcagtcaacc gactcctcgc acacgcattt
     3241 cccgatgaag tgcgaccgtg gatgtcgagc cacgttccga ccggttactg ggaagatgaa
     3301 aaccatcgcc ggaatgccgt tcggtggctt gtggaggagt gtatcggcgt acaacccgat
     3361 gcgattgccg aagcgattca cgcgggccgt atcaccaaga aagacttcgc ggatgcgggg
     3421 ttaacgtggt tggtgaagga ggtgtaccgc tggtcggttg ccgacgcgct cgccgaagcg
     3481 tatccgacgt tggaaccgtg ggaacggctc cgccgactac ctaccgagtt ttggcgcgtc
     3541 gacgatggtg gggcgaccgc cgcccgtgcc atccggtggg cgctggatcg aggtgaagtt
     3601 ggtgtcgacg aattgcgcca ccaaaaggcg agtcgaacga ttgccgccgc gctacgtccg
     3661 tggaaactcg tctcggcgtt ctcagtcgga ttcgacggcg atgcgttccg gtgcctcgat
     3721 acactctttc cgaacacctt ccggccatgg gaagtggcaa acgtaccgcg cgacgactgg
     3781 aaggacgcga aactccgtca gaccgcacta ttctggcttc tcgatcggct cggaatcgat
     3841 ccgtcggaga ttcctgcagc aatcgctcaa ggccgtttga cgccggcaag cttcacagac
     3901 aatggactcg acggacttct gcgcgtaacg ggaagcgtgt ggcgcgtggt gtgcgacgta
     3961 ttcccggatc aattcgctcg gtgggaactc ggtacggtcc cgcgcagcca ctggcgaagt
     4021 cgcgcgaatg ttcgtgaggc ggtgctatgg gcgatgaagc ggctcggtat ctcggagcaa
     4081 agcctcggtt cagcgattcg cgacggccgg atgacgacga acgcactggt gaacctcgga
     4141 ctcggttcgc tgatcgtcgg cgtattccgc ggagatgtta ccgcgatgtg caaagtcgcc
     4201 gacgtgctac catcggagag ctacgtgccg ctatcccggt attaccgaca aagtgcaagt
     4261 ggacaatccg ccgaccgcgg cgcgtcgcgc cttgacgccg cacgacgccg agcgcagtct
     4321 gatctgatga acgagagcga actcgatcga caccttagcg tgtcacgttc cattcgtgag
     4381 caacgtcggc gtcgaacaga ctgaagcatc taccagacct cgtcgatcac ttcgcgcgca
     4441 atcctcaccc agtcgctcac gcggttcggg tcgccttcca ccgtttcgac gtccttcagt
     4501 gtgatatcga cgtggagtcc gcgacacgct gagaggtcct tacggaggct atcacggatg
     4561 tagtcggcgt cgaatcccgt tgcgatcatc gccggattcg ggcggtagct tatgacgtat
     4621 tcggtgccga tacgttccgc cgaagccgcg acattcgcag aaggcgcaac cgcgatgcgg
     4681 cgcaggttcg ggatcgcgcg aatcgcctca atcttgtgtg taaggtcttc gcaacagccg
     4741 tacgcgacga gtccgaacgg cctcatgatc gggatctggt attcgagcat gaactcattg
     4801 aacattgttg gtccgacccc ggtcgtctcc tgtgaagcgc agtaatacca gaggtgtttg
     4861 cgcttgaccg actcgccagt tggagtcgta ctgcttggtg ccggatcggg taattcggtg
     4921 gcgtacggca tcgattgatt ctggtggttg atcagcgacc aatctcccac ctcttccgcc
     4981 tgttcgtgag tccgtatgat gccgtcgcgc atgaacgtga gaaggcggtg tagccactca
     5041 gggcgatcga tcatatcgag catgatttgg tcgaggccgc gcaggtacgc gatctgcgtg
     5101 gagatgtcgc cgttccacat gcggtattcc ggagcgcggt cagcgaacac ggggaggatg
     5161 tcgccaaaca cctcgatcgt gcgcgcaagt cgctcggcag tgcgctcttc gtcgatgacg
     5221 tggtgcggcg gaatcagttt ggtgaaatca tcttcggtgg cgatcggcgg tatccacttc
     5281 tttgcgccgc gtggtgttct gctgtcgatc gccgtcggac tcacgcccca tacgcctccc
     5341 gcaggcgtga tgacggcagc gcgcgcggga atccacggct cgaataccga atcgtcatta
     5401 agcgacgccc agaatagctg tcgacggagc cagtcttcat atccgcgaaa aaaggggtct
     5461 tctacggtga gttccgcatc cggcatttca caccatgcga acgcgcgggt gtagatcagc
     5521 ggtctcgtcg caatgaggct gttgtgcgcg cgccagaggt cgcggcattc cgcgttcaca
     5581 tcgcgcgcac atacgtcgac atagcgcttt gccagttcgc gcaacacgta gcgatctgat
     5641 tgtgtaaagg acatacgcgc gctattcctt cgctcggaga acgagcaccc aatcgtgcac
     5701 gacgacagga tgcggtgccg tccacgagcc gtcgggatcg ggtgtcgcgt cgccaatgtc
     5761 ggtccgctcg ccgttcaccg gatcaaacca gaacgccgtg tatgccacat cggattcgag
     5821 accaagtacc ttcgagacgt tccacgtgtg cggcatgtag acaacacgga gcttccgtgg
     5881 gattcccgcg cagatgttcc ggtccggatc ggagccgtcc cacgggtttt ctacccactc
     5941 attgtgcggc tcgagctccc accacggaat cgtatcgagc agtcgtcgtg cgtagccgac
     6001 ttgccgtgaa ccgggaagct ggtaggcctc ctgccatgtc gcttcgcccc atgaacggcc
     6061 atgtggcgac ggaccgtacg ggtcatcagt gcggctcatt tgccagatgc cgtttgcacc
     6121 gtacgtgtgc ccaaatgcgc cgctcagcac cggctgccag aatgacgcgc gctgaacatc
     6181 ctcccacgaa cgaccgagga tgccttcgta attcacctcg ctaacgtacg tcggcatggt
     6241 cggctcttcg gcgtacgcgg tccgcaccgt ttcgacgttg ttcacgaggc tgaggcgaga
     6301 gctgtggccg gtctggagca tctcgaaatc gagcacgccg gggtcggtga cttgcttacg
     6361 gccgtattgc gtcggatgaa tcgtgatcgg gttgcgccat ccgtcgatag agcggacgta
     6421 tcgggcgact tcggtccagc cttcgagttg ctgcttcgcc tcggcttcgc ggttttcgga
     6481 gagataccac gccatggtgc cttcccccgc gatgcaccag cagaccgggt acgcaccgta
     6541 gcgcgcgacg atgtaccgcc agtaccgttt gattcgttca acgccgatct ccgggaggta
     6601 gtagccccac atcccgacga tgcacggcat gagtccgacg c
//
LOCUS       DSCC01000356            4274 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1013882, whole genome shotgun sequence.
ACCESSION   DSCC01000356 DSCC01000000
VERSION     DSCC01000356.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1013882"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            122..457
                     /locus_tag="ENN56_04460"
     CDS             122..457
                     /locus_tag="ENN56_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74775.1"
                     /translation="MKQRGIEITNVIDRRLVAYVGDTLPDTLDMVDFAGSEVVIVEST
                     FLEPGHFELAEARGHLHLQHIIERIDTFGDAHIVLTHFSRRYGRSDIESLIRSQWPEN
                     HRDRLSLLV"
     gene            525..1733
                     /locus_tag="ENN56_04465"
     CDS             525..1733
                     /locus_tag="ENN56_04465"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS74776.1"
                     /translation="MNRSALTVLALTAFVDMLGFSIILPILPFYAETYGATASVIGLL
                     FASYSIMQFIFSPIWGSVSDRIGRKPVLIIGLTGAAIGLIIFGLAESLFVLFAARMFS
                     GIMASTVPVAQAYVADVTAPEYRAKAMGMLGAAIGLGFVLGPALGGMLEGFGRGLPAF
                     VAAGIAILNAIWTATALGESLSHETRSERWLPLGAVKTIVKLPLVTLILGVFFINVFA
                     FAAMEATFALLIRDVVFPGVSHAVLARRVGYLLGFSGVIMVIVQGGLLGRLVRAFGEA
                     RLIPTGLAFIAGGLVMLPLLTSLVWVMVSLTLLSVGVALIRPNIFSLLSQLVPESTQG
                     GIMSIGQALSSLARATGPAWAGWMYHAVGYPAPFIFGSVLTGIAFGGALVIVAILPAT
                     FRRVGFRVSA"
     gene            complement(1739..2095)
                     /locus_tag="ENN56_04470"
     CDS             complement(1739..2095)
                     /locus_tag="ENN56_04470"
                     /inference="COORDINATES: protein motif:HMM:PF13860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74777.1"
                     /translation="MSFIWRRPSPPQMFQYTFHCVNHPGRFVASFEVSGDNVVLIGFD
                     LVGALPHRVEINIYNRAGYLVAQPFTNNQLKPGKYSIVWQGTDANENNVAPGIYIVEY
                     RTERDSTSQIIRWPLE"
     gene            2119..3000
                     /locus_tag="ENN56_04475"
     CDS             2119..3000
                     /locus_tag="ENN56_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74778.1"
                     /translation="MYVELNSGDLSAAVVDNAAHHELSGHRAGYNGLAWLKSVHRHEN
                     LFVPLYAGLNLELYFDGTDTERKRLFEPRSAPMSIRQVDDTTAILYQEPTPHFRVESW
                     TTFQLIAPHSIDFHFRAIPRARTFNGPMGVFWASYIEQPEDLALTFPVIDEHGERWVR
                     HLSPQHGVESTHRFPHDTIDLPASGMHEQYMYASLSKWRLARPYYFGVSNGMMYLFMA
                     DTQHPSDDTIVRFTQSPSGGGPRCPAWDFQMIVPEYEIDTPYGISARVVYKPFTSYRD
                     IEDEFAQWQAHRFGTRP"
     gene            3429..>4274
                     /locus_tag="ENN56_04480"
     CDS             3429..>4274
                     /locus_tag="ENN56_04480"
                     /inference="COORDINATES: protein motif:HMM:PF00709.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDS74779.1"
                     /translation="MAVTVAVGTHWGDEGKAKIVDFLAQRHDAVVRFQGGANAGHTVI
                     VDGKKFIFHQIPVGLIHPGKTCVVGNGVVFDPMEFLKELDEHTSTGIDPKDRLWVSDR
                     AAVVMPWHKRIEAIREDAAGEGAIGTTKRGIGPTYLDKISREHGIRVADLLYPKSFRD
                     CVRAIVSDKNDVFTKVYGAEPLDADKIADDYLNAAERLRPFITNTSALLHDMIRSGKR
                     ILCEGAQATLLDIDHGTYPFVTSSNTTAGGSVTGTGIGPTYISDVVGIVKAYTSRVGN
                     GPFPTE"
BASE COUNT          923 a         1105 c         1193 g         1053 t
ORIGIN      
        1 tcttcttgtt caaccgttca gcgtacacca tacgttacca tcggttggat atacggttat
       61 tcagcgaaca cgaaaacttc ggtcggagta tctcggcatt gccggatatg aactcgctgc
      121 gatgaaacaa cgcggtatcg agataacgaa cgtgattgac cggagactcg tggcatacgt
      181 cggtgatact ttacctgata cattggacat ggtcgatttt gccgggagcg aggtcgttat
      241 cgtagaatcc acgttccttg agcccggcca ttttgaactt gcggaagccc gcggtcatct
      301 gcatctccag catatcatcg agcgtatcga tacattcggc gatgcacaca tcgtactgac
      361 gcacttcagc cgacggtatg gtcgctcgga catcgagtcg ctcatacgct cgcagtggcc
      421 ggaaaaccat agagatcggc tcagcctcct cgtatgacga cgatcgctta tctcaactcg
      481 cgacattacc gaatactttt cacacccacg ggaatcttcg ctgaatgaat cgctctgcgc
      541 tcactgtgct tgcattgacc gcgtttgtcg acatgctcgg cttcagcatc atcttgccga
      601 tattaccgtt ttacgcggaa acgtacggcg cgacggcgtc ggtgattgga ttgttgttcg
      661 catcatattc gatcatgcag ttcatttttt cgccgatttg gggaagcgtg tccgaccgaa
      721 tcggacgaaa gccggttctc atcatcggac tgaccggtgc ggccattgga ctcatcatat
      781 tcgggcttgc ggaaagttta tttgtcctct tcgccgcacg catgttttcc ggaatcatgg
      841 cgtctacggt tcctgttgct caggcatacg tagcggatgt taccgctccc gagtaccggg
      901 caaaggcgat gggtatgctc ggagcagcga tcggtcttgg attcgttctt ggaccagcgc
      961 tcggcggcat gctggaagga tttgggcgcg gtctgccggc attcgtggcc gccggtattg
     1021 ccattcttaa cgcgatatgg acggcgacag cgttagggga atcgctttcg catgagactc
     1081 ggtccgaacg ttggttgccg ctcggtgcgg tgaagacgat cgtaaagctg ccgctcgtca
     1141 cgctaatcct cggtgttttc tttatcaacg tatttgcatt tgcggcaatg gaagcaacgt
     1201 tcgctttgtt gattcgagat gttgtctttc ccggcgtatc gcacgctgtt ctcgcaagac
     1261 gggtgggata tcttctcggg ttttccggtg tgatcatggt aatcgtacaa ggaggtcttc
     1321 ttgggaggct cgtacgagcc tttggtgagg cgcggctgat acccaccgga ctcgcgttca
     1381 tagcgggcgg gttagtaatg cttccacttc taacctcgct cgtatgggta atggtttcgt
     1441 tgacactgct cagtgtcggt gtcgcactaa ttcggcccaa tatcttcagc ctcttatcgc
     1501 aacttgttcc ggaaagtaca caaggtggga ttatgagtat cgggcaggca ctctcaagtc
     1561 ttgcgcgagc aaccggcccc gcatgggccg gttggatgta tcacgcggtg ggatacccgg
     1621 cgccgtttat tttcggaagc gttctcactg gtatcgcgtt cggtggcgca ctcgtgattg
     1681 ttgcgatctt accggcaaca tttcgtcggg tcggttttcg agtatccgcg taagagcatc
     1741 actccaacgg ccatcgaata atttggctcg tgctatcacg ttccgttcgg tattcaacga
     1801 tgtatattcc tggcgcaacg ttgttttcgt ttgcgtcggt tccttgccag acgatgctgt
     1861 atttccccgg cttgagttga ttattcgtga atggttgtgc aacgagataa ccggcgcggt
     1921 tgtatatgtt gatttcgact cggtgcggca acgcgcctac aagatcgaag cctatcaata
     1981 ccacgttatc tccacttacc tcaaaagacg caacaaacct ccctgggtga tttacacagt
     2041 gaaaggtata ttgaaacatc tgtggtggcg acggtcgacg ccatatgaag gacatcgtcg
     2101 acacggttgg gagacgcgat gtacgtcgaa ttgaattccg gcgacctcag tgctgctgtt
     2161 gttgataatg cggctcatca tgagctttcc ggtcatcgtg ccggatacaa cggccttgcg
     2221 tggttgaagt cggtacatcg acatgagaat ctttttgtgc cgctctacgc aggtttgaac
     2281 cttgagctct atttcgacgg cacggacacg gaacgcaaac ggttgttcga gcctcgttct
     2341 gcgccgatga gcattcgtca agtcgacgat acaaccgcga ttctgtatca agaaccgacg
     2401 ccgcatttca gagtcgaaag ttggacgacg tttcagctta ttgcaccgca ctcgattgat
     2461 tttcactttc gagcaatacc acgagcgcga acgttcaacg gtccgatggg tgttttttgg
     2521 gcgagctaca tcgaacaacc ggaagacctt gcgctgacgt ttccggtcat cgacgaacac
     2581 ggcgagcgtt gggttcgtca tctatcgcca caacacggcg tcgagagcac gcaccgcttt
     2641 ccacacgaca cgattgattt gccggcgagt ggcatgcacg aacagtatat gtacgcatca
     2701 ctttcgaagt ggcggctcgc gagaccatat tacttcggcg tttcaaacgg aatgatgtat
     2761 ctgttcatgg ccgatacgca acatccgtcc gacgacacga ttgttcggtt tacgcaatcg
     2821 ccatcaggcg gcggtccgag gtgcccggca tgggatttcc aaatgatcgt tccggaatac
     2881 gaaattgata cgccatacgg gatctccgcg cgggtcgtat ataagccgtt tacgagttac
     2941 cgggatatcg aagatgagtt cgcgcaatgg caagctcacc gattcggaac acgaccataa
     3001 aatcgattcg ccgtgcgaaa gggaaactgg tcttgacggc accttgtgtc ccgtatgcgg
     3061 gcgggtcgtc gagtcgctcc cagtaagact ccatcgtttc cgaggcgatt tcgaagagta
     3121 cttgtgggtg tgctccgacg aatgccggag tcatgccggg caatcaccgt acgtgcagac
     3181 gaatctgttc ggacgagatc tacgcgtctg ggctcgcagt gtgcagggag agcttcctct
     3241 tctcgcagaa ggaggaaacg gagacgggcc tacgaaagaa aatgaacatc agaacgattg
     3301 aatcggtcgt tgtttgcaga tagcctcgta ctctcgctat attcagacac ggcccgagct
     3361 gcccaagggc agccgggtcg cttttttgtg ttagcaccaa cagtccgtat ccgaaaagag
     3421 gtaaaaccat ggcagtaacc gtggcggttg gcactcactg gggtgacgaa ggcaaggcga
     3481 agatcgttga tttcttggcg caacgccacg acgccgtcgt tcggtttcaa ggcggcgcga
     3541 acgccggaca cacggtcatc gtcgacggaa agaagtttat ctttcaccaa ataccggtcg
     3601 gtttgattca tcccggaaaa acgtgcgtcg ttggcaacgg tgttgtcttt gatccgatgg
     3661 agttcctcaa ggaactggac gaacacacgt caaccggaat cgatccgaaa gaccggctct
     3721 gggtatccga ccgcgcggca gtggtcatgc cttggcacaa gcgaatcgaa gcgattcgtg
     3781 aggatgctgc cggcgaaggc gcgattggta cgacgaagcg cggtatcggt ccgacgtatc
     3841 ttgataaaat atcacgtgaa cacggcatca gggtggccga cctcctttac ccgaagagct
     3901 ttcgagactg cgttcgcgcg attgtgtcgg acaaaaacga cgtcttcacc aaggtatacg
     3961 gtgcggaacc tctcgatgca gacaagatag ccgacgacta tttgaacgcg gcggaaagac
     4021 tccggccgtt tatcacgaac acgtcggctc tgctccacga tatgatccga tcgggaaagc
     4081 gtattctctg cgaaggtgct caagcgacat tgctcgacat tgatcacggt acgtatccgt
     4141 ttgtcaccag ttcgaacacg accgcgggtg gctccgtaac gggtacgggt atcggtccaa
     4201 cgtacatctc cgacgtggta gggatcgtca aggcgtatac gagtcgtgtc ggtaacgggc
     4261 cgttccctac ggaa
//
LOCUS       DSCC01000357            3032 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022056, whole genome shotgun sequence.
ACCESSION   DSCC01000357 DSCC01000000
VERSION     DSCC01000357.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022056"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..625
                     /locus_tag="ENN56_04485"
     CDS             <1..625
                     /locus_tag="ENN56_04485"
                     /inference="COORDINATES: protein motif:HMM:PF02517.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDS74780.1"
                     /translation="YYGYPLTYILHFDALIGDWRYRSIAPSLYWSGSNVVLLLVIPLI
                     FARTLGWKPRQLGLGLGNKRLGLLVVAIGWLIMLPLVIGVASTGDFRGTYPLERGALE
                     SHELFWVYETGYMSFFVGWEFFFRGFLLFTLEPFFGPVAIAVQMIPFALLHFGKPVLE
                     ALGSIIAGVFLGAVAWRTRSIWYGVALHALVALTMDFYVYFRWGLSL"
     gene            complement(631..1407)
                     /locus_tag="ENN56_04490"
     CDS             complement(631..1407)
                     /locus_tag="ENN56_04490"
                     /inference="COORDINATES: protein motif:HMM:PF07859.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS74781.1"
                     /translation="MVFDTDLRFGDWERGLLDVMTPAGGGPFPVVVCVHGGGWRFGAK
                     DDMHPYAEWLLSLGIASVLPNYRLSDEAIHPAQQDDIIAALNWIADHADEYHFDVDRI
                     GLTGVSAGGHLSAQVGCLAKERAPRVHVRCMYPVCPPTDFPQFIADNPGIGQTIEQFL
                     GASLADLSDMLKDVSPVTHVHADAPVCRCVHGDLDELVPPNQSRIFVEALRNAGVEAD
                     VLRVPHAGHAAYQPNVEPLEPLGGLESYLGFFRKHLVEAQ"
     gene            1713..>3032
                     /locus_tag="ENN56_04495"
     CDS             1713..>3032
                     /locus_tag="ENN56_04495"
                     /inference="COORDINATES: protein motif:HMM:PF00089.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HDS74782.1"
                     /translation="MQWRKSVVGATLIGAGIFIGLLFSAESSITDASPVAPQVAMAQQ
                     PSVPQSVEPTVRQGMEAAQDLSRAFEWVAEQVTPSVVTIRSEKTVRRQMPELPDFFPH
                     EFFRMPRRGTPEEEFRSQGLGSGVIVSTDGYILTNNHVVENADKIIVLIGPESDEVEA
                     EIIGTDPWTDIAVIKVKRTGLPAIRMGDSDRLRVGNWVLAIGSPFGLRLQHTVTAGIV
                     SALNRSIGLIEPDQSYTGFERFIQTDAAINPGNSGGALVNLNAELVGINTAISSTTGA
                     NAGIGFAIPVNMARQVMRQLIENGRVRRGYLGVQIGEVTSEFRDALGLPNRNGAFVQS
                     VSEGTPAAEAGIKPGDVIRTVDGEAVLNVPELRYRIASTGPNKQITFGIWRDGRMIDV
                     RVTLQEVPEELGGGEVPRVAAADASTSTRLGMSIRTLTAQLARQYGYE"
BASE COUNT          655 a          843 c          887 g          647 t
ORIGIN      
        1 gtactacggg tatccgctga cctacatcct ccatttcgac gcactcatcg gcgactggcg
       61 ttaccgctcc attgcgccga gtctgtattg gagcggctca aatgtcgtct tgttgctcgt
      121 tatcccgctt atcttcgcac ggacactcgg atggaagcca cgtcaacttg ggctcggact
      181 cggaaacaag cgcctcggtc tcctcgtagt ggcgatcggc tggttgatta tgcttccgct
      241 cgtgatcgga gtcgcatcga ccggtgattt ccggggcaca tacccattgg aacggggagc
      301 actcgaatcg cacgaactgt tttgggtcta cgaaacgggg tatatgagct tttttgtcgg
      361 atgggagttc ttcttccgcg ggtttctctt gttcacgctc gagccgtttt ttggtcccgt
      421 ggcgatcgct gtccagatga ttccgtttgc actacttcac ttcggaaagc ccgttctcga
      481 agcactcggt tcgatcatcg ccggcgtgtt tcttggtgcg gttgcgtggc ggactcggtc
      541 gatatggtat ggtgtcgcgt tacacgcgct tgtcgcactt accatggact tctacgtgta
      601 tttccggtgg ggtctaagtc tgtaaatgcg tcactgcgct tcgacgagat gtttgcggaa
      661 aaacccgaga taactttcca gaccgcccag tggctccaac ggttcgacgt tcggctgata
      721 cgctgcgtgg cccgcatggg gaacgcgtaa cacatccgct tccactcccg cgttccgcag
      781 ggcttcgacg aatatccgcg attggttcgg tggcactagt tcatccagat caccgtggac
      841 gcaccgacag accggagcat cggcatgaac gtgcgttacc ggcgatacat ctttcagcat
      901 atccgaaaga tcggcgaggc tcgcaccgag gaattgctcg atcgtttggc cgattccggg
      961 gttatccgcg atgaactgag ggaaatccgt tggtgggcat accggataca tgcaccgtac
     1021 gtgaacccgc ggcgcacgtt ctttcgcaag acagccgact tgagctgaga ggtgcccgcc
     1081 ggccgataca ccggtcagac caatacgatc cacgtcgaaa tggtattcgt cggcatgatc
     1141 ggcgatccaa ttgagagccg cgataatatc atcctgttgc gccgggtgaa tcgcttcgtc
     1201 cgacagtcgg tagttgggta gtaccgatgc gattccgagc gataaaagcc attcggcgta
     1261 cggatgcatg tcatccttgg caccgaatcg ccacccaccg ccgtgtacgc acacaaccac
     1321 cgggaacgga ccgccgccgg caggtgtcat cacgtcgagc agaccgcgtt cccagtcgcc
     1381 aaaccgcaga tcagtatcga aaaccatggt cactccgaaa acgttaggcg tgtcgcgtcg
     1441 acgcgttcaa actaccgttt gcgtttacac ggaaatggta acaccaatac acggaagaag
     1501 ccagccgatg aagtggaggt atgcggacgc gcggaatcac gattcggaac cagatttggt
     1561 ggcaacgcgt caacgtaaac agcgcgcgga tgaatggtca gcaccgccgt gtagagttgc
     1621 acggaatcct aagcagtttt cggtttgctc gatggatttg aatcgttcgt atacgcgcga
     1681 tatagaaaca ctacaaacga ggagacccgc gaatgcaatg gcggaagtca gttgtcggcg
     1741 cgacactcat tggagcaggc atattcatcg gattgctgtt ttcagcggaa agtagtatta
     1801 ccgatgcgag tcccgtagcg ccgcaggttg cgatggcaca gcaaccatcg gtgccgcaga
     1861 gtgtggaacc gaccgttcgt caaggaatgg aagcggcaca ggatttgagc cgcgcgtttg
     1921 aatgggtggc tgaacaagtt actccatcgg tcgtcacgat acggagtgaa aagaccgttc
     1981 gtcggcaaat gcccgaactc ccggactttt tcccacatga gttcttccga atgccgcgcc
     2041 gtggcactcc cgaagaggag ttccggagtc aagggctcgg ctccggcgta atcgtaagca
     2101 cggacgggta catactcaca aacaaccatg tcgtcgaaaa cgccgacaag atcatcgtac
     2161 tcatcggtcc ggaaagcgac gaagtcgaag ccgagattat cggcacggat ccgtggaccg
     2221 atatcgcggt aatcaaagtg aagcggaccg gactccccgc aattcgtatg ggcgacagcg
     2281 accgacttcg cgtcggaaac tgggtgctcg caatcgggag cccgtttggc ttgcgactcc
     2341 aacatacggt taccgccggc attgtcagtg ccctgaatag atcgatcggt ctcatcgaac
     2401 cggatcagag ctataccggt ttcgagagat tcattcaaac cgatgcagcg atcaatccgg
     2461 gtaactcagg tggcgcgctc gtaaacctca acgccgagct tgttggtatc aacaccgcga
     2521 ttagcagcac gactggtgcg aacgcgggta tcggattcgc aattcccgtg aacatggcgc
     2581 gacaggtcat gcggcagctc atcgagaacg gccgcgtgcg tcgtgggtac ctcggtgtgc
     2641 agatcggtga ggttacatcc gagttccgcg atgcgctcgg ccttccgaat cgcaacggcg
     2701 ctttcgtgca gagtgtgagc gagggaacgc cggccgccga agcaggcatc aagcccggcg
     2761 atgtcattcg caccgtcgat ggcgaagcgg tactaaacgt gccggaatta cgctaccgta
     2821 tcgcgagcac cggtccgaac aagcagatca cgttcggcat ctggcgggac ggtcggatga
     2881 tcgacgttcg cgtgacactg caggaggttc ccgaagaact cggtggcggc gaagtaccgc
     2941 gtgtcgccgc agccgacgcc tctacgtcga cgcggcttgg tatgtcgatt cgtacgctta
     3001 ccgcgcaact ggcacgtcag tacgggtacg aa
//
LOCUS       DSCC01000358            1689 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048454, whole genome shotgun sequence.
ACCESSION   DSCC01000358 DSCC01000000
VERSION     DSCC01000358.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048454"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="ENN56_04500"
     CDS             complement(<1..1021)
                     /locus_tag="ENN56_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDS74783.1"
                     /translation="MALKDLRGQKTRRTPEEMIEHLKTFELDLKFSAGVWFFAPGGGR
                     FHGRYQEEMTIKQRLEIAAELAEYGLKGVEAHYPNEVNEDNLHLYKELEKNSGVKLIT
                     VIPNLFYDSRYEWGSLSSPIKEVRQHALDRTIAALKINKELDTDFAIVWPGGDGYENG
                     FGLDMVAARDRFAKGLADAMDAVPGVRVAEEPKPYEPRGHIFYGTTPEGVLLCEKVES
                     YLTAEENKKLLKEGHALMTMNPEVGHMLMGYEDPPYSFSLIMEFGRLAHTHWNSQPLG
                     NYDQDLNVGVLSPESTESAMYALKMHGYNGYFGPDINPERMPIRQALKNNFDALRAVN
                     ERINNL"
     gene            complement(1210..>1689)
                     /locus_tag="ENN56_04505"
     CDS             complement(1210..>1689)
                     /locus_tag="ENN56_04505"
                     /inference="COORDINATES: protein motif:HMM:PF00588.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HDS74784.1"
                     /translation="VYDPHNVAAILRSVDGFGIAQVNLYYTIENFPDFGKAGTKSSAS
                     ARKWVRRERVEDLSTFVADRKAAGYRFVGADRTGGSIALPEYSFADRCIIALGSEHHG
                     MQPEVVAALDDTVHIPMTGMVESYNVSVAAAVIIYEVFRQHGRGLISREELGVNEAR"
BASE COUNT          336 a          523 c          455 g          375 t
ORIGIN      
        1 cgaggttgtt gatccgctca ttgacggcac gcagcgcgtc gaagttgttc ttgagcgctt
       61 gacgaatcgg catccgctcc gggttgatgt ccggaccgaa gtagccatta tagccgtgca
      121 tcttcaatgc gtacatcgca gactcggtcg attccggtga gagaacaccg acgttcaggt
      181 cctggtcgta gttaccaagc ggctggctgt tccagtgcgt atgcgccaga cgaccgaact
      241 ccatgatcag gctgaagctg tagggcggat cctcgtatcc catgagcatg tgacccactt
      301 ccgggttcat ggtcatgaga gcgtgacctt ccttgagcag cttcttgttc tcttcggcgg
      361 tcaggtacga ttcgaccttc tcgcagagga ggacaccttc gggcgttgtg ccgtagaaga
      421 tgtggccacg cggctcatac ggtttcggct cttcggcgac acgcacgccc ggtacagcgt
      481 ccatcgcatc ggcgagcccc ttcgcgaacc gatcgcgcgc ggcgaccatg tcgagcccga
      541 atccgttctc gtatccgtcg cctcccggcc agacgattgc gaaatccgtg tcgagctcct
      601 tgttgatctt gagagcggca atcgtccgat cgagcgcgtg ctgacggact tccttgatcg
      661 ggctcgaaag cgagccccac tcataccgcg aatcgtagaa gaggttcgga atcaccgtga
      721 tgagctttac gcccgagttc ttctcaagct ctttgtagag gtgaagattg tcttcattca
      781 cttcgttcgg atagtgcgct tcaacgcctt tgagaccgta ttcagcaagc tccgcagcga
      841 tttcgagccg ctgtttgatc gtcatttctt cctgatagcg accatggaaa cggccgcctc
      901 cgggagcgaa gaaccataca ccggcggaga atttcagatc gagctcgaat gtcttcagat
      961 gctcgatcat ctcctcaggc gtacggcgcg ttttctgtcc gcgcaggtct ttcaacgcca
     1021 tcgtatcaac tccttacttc tcgtgtgtgt ttaccgaccg tccggatata caaccggtcc
     1081 gccatagtat ctcaacgtgc aacacgtgca acgtgcaaca cgcgcaacgt gcaacacgcg
     1141 ccccgaatat acaaagtacc cgcgcatcga tgaaggcgcg agcaacctca acatgcttcg
     1201 ccgacaccat caccgcgcct cgttgacgcc caactcttca cgggagatca agccacgtcc
     1261 gtgctggcga aaaacttcgt agatgattac tgcggcggcc accgatacgt tgtagctctc
     1321 gaccatgcca gtcatcggga tgtgcaccgt gtcgtcaagc gcggcaacca cctccggctg
     1381 cataccgtgg tgctccgatc ccagggcgat gatgcatcgg tcggcaaatg aatactccgg
     1441 caacgcgatc gacccaccgg tgcggtccgc gccgacgaat cggtatcccg ccgccttcct
     1501 atccgcgacg aacgtggaga gatcctcgac gcgttcccgg cgaacccact tcctcgcgct
     1561 cgcactcgac ttggtgccgg ctttcccgaa atccggaaag ttctcgatgg tgtaatacag
     1621 gttcacctgt gcgatgccga acccgtccac acttcgaagt atcgccgcga cgttgtgcgg
     1681 gtcgtatac
//
LOCUS       DSCC01000359            2673 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026023, whole genome shotgun sequence.
ACCESSION   DSCC01000359 DSCC01000000
VERSION     DSCC01000359.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026023"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="ENN56_04510"
     CDS             complement(<1..1209)
                     /locus_tag="ENN56_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDS74785.1"
                     /translation="MSLTYLIFGPAIAGIVLGFLPEKRRGLVRYVALLLSLGVFGYSL
                     GIYLTFRATEIPIDFNVLWIRDFGIHYHLAVDGLSIWLVLLTTFLTPLAILASFRSVN
                     DRVRLFNSMLLLLEAGMLGVFLAQDLILFYAFWELMLVPMYFIIGIWGYDERIKATIV
                     FVIYTMVGSLLMLVAMVSLAIVNAQNLAMRLGGSLWDWLSFDLGRIDPTLLTPDQQVW
                     LFAAFFFAFMIKVPLFPVHTWLPLAHVQAPTAGSVILAGVLLKTGAYAIIRFCLGLFP
                     AASMQFAPWINTLAVIGIIYGALVSLVQTDFKKLVAYSSVSHMGFIILGIFSFTVEGI
                     TGGIVQMVNHGISTGGLFLCVGMLYERSHTREFSELGGAATAMPVYAGLFLFITLSSA
                     ALPGLNGFIGE"
     gene            complement(1287..>2673)
                     /locus_tag="ENN56_04515"
     CDS             complement(1287..>2673)
                     /locus_tag="ENN56_04515"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDS74786.1"
                     /translation="AFIVNRIGDFGFLIGVFLIWTTAGSVSFSDIGTAAAGGVFVAGG
                     ALITTITLLLFFGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARSS
                     FLYDLAPLSQGIVLWVGALTALFAASMGVAQNDIKKILAYSTISQLGYMFMGVGVGAY
                     ASGVMHVMTHAFFKALLFLGAGAVIHALHEEQDITRMGGLKKHLPLVYWTFLAGALAL
                     AGFPLTAGFFSKDAILAFIFSEGHFGAWAVGVFTAGLTAFYTFRLVFLTFHGPERNDA
                     HIHKPGPSIAVPLAVLGVLSIIGGYFEIPLHIWAGFSGFLAPVVGEAHVALTVATEII
                     LMVVTLALVLLGIYGAWVLYAKRPVDVARADAIAAEPTGFLRILRHKWYVDELYDAVI
                     INPMKNISRTVLWRVVDDWIIQGFFVQFVGGLMWKAFGFVISFWHTGRVGSYAFGMVL
                     GVLLLLWMALK"
BASE COUNT          736 a          760 c          695 g          482 t
ORIGIN      
        1 ctccccgatg aatccgttca agccgggtaa cgccgcgctg gataacgtga tgaagaggaa
       61 taaccccgcg tataccggca tcgccgtcgc cgcgccgccg agttcactga actcgcgtgt
      121 atgtgaacgc tcgtagagca tgccgacgca aaggaacaat ccaccggtag atattccgtg
      181 gtttaccatc tgaacgatac cgccggtaat cccttcgacg gtgaacgaaa agatccccag
      241 gataatgaaa cccatgtgac tcaccgacga atacgccaca agcttcttga aatccgtctg
      301 tacgagcgaa acgagggctc cgtagataat cccaatcacc gcgagcgtgt tgatccacgg
      361 tgcaaactgc atcgacgcgg caggaaaaag cccgaggcag aaccggatga tggcgtacgc
      421 gcctgtcttc agtaagacac ccgcgaggat aaccgatccg gcggtaggtg cctgaacgtg
      481 cgcgagcggt aaccacgtat gcacggggaa caggggcacc ttgatcatga atgcgaaaaa
      541 gaacgctgcg aaaagccaca cttgctggtc tggtgtcaga agcgtcggat caatgcgtcc
      601 aaggtcgaac gagagccaat cccaaagcga tccgccgagt ctcatcgcga ggttttgcgc
      661 gttgacgatc gcgagtgaaa ccatcgcaac gagcatgagt aatgatccga ccatcgtata
      721 aatcacgaat acaatcgtcg ctttgatgcg ttcgtcgtag ccccatattc cgatgatgaa
      781 atacatcgga acgagcatca gctcccaaaa cgcgtagaaa agaatcagat cctgcgcgag
      841 gaatacaccg agcataccag cttcgagaag aagcagcatc gaattgaaga gtcggactcg
      901 atcgttaacg cttcggaagc tcgcaagaat cgcgagcggt gtaaggaacg tcgtcaacaa
      961 cacgagccag atgctcaagc cgtcgactgc gaggtggtag tgtatgccga aatcccgaat
     1021 ccacaagaca ttgaaatcaa tcggtatttc ggttgcacga aacgtcagat aaataccaag
     1081 cgaatatccg aatactccaa gcgagagcag cagagccacg taccgaacca gccccctgcg
     1141 cttctccggg agaaagccaa gaactatacc ggcgatcgcc ggcccgaata tgagatacgt
     1201 caaactcatt gcgttggatt tctccgttgt cggcgactgc actttacgcg ctcgtcgcct
     1261 cgctcagatc tcattcgcgg tttattttat ttcagtgcca tccatagcag gagtaataca
     1321 ccgagcacca taccgaaagc gtagcttccg acacggcccg tgtgccagaa actgataaca
     1381 aaaccgaacg ctttccacat caatccgccg acgaattgca cgaagaatcc ctgaatgatc
     1441 caatcgtcca caactcgcca caaaacggta cgtgagatat tcttcatcgg attgatgatg
     1501 accgcgtcat acaactcatc gacataccac ttgtggcgca gtatccgcag aaaccccgtc
     1561 ggttcggccg caattgcatc cgcccgcgcg acgtccacag gacgtttcgc atacaacacc
     1621 caggcaccgt agattccaag caagacgagg gcgagtgtca ctaccatgag gattatttcg
     1681 gtagcgactg tcagtgcaac gtgagcttca ccaaccaccg gtgcgaggaa tccagagaaa
     1741 cccgcccaaa tatgcagcgg tatctcaaag tagccaccga tgatgctgag tacaccgaga
     1801 accgcgagcg ggaccgcaat agacggaccg ggtttgtgga tatgtgcgtc gttccgttcg
     1861 ggaccgtgga atgtcaagaa cacaagtcgg aaggtgtaaa acgccgtaag tcccgccgta
     1921 aagacgccta cggcccaagc gccgaagtgt ccttcgctga agataaacgc gagaattgcg
     1981 tccttcgaaa agaagccggc cgtcaagggg aatccggcga gcgcaagcgc tcccgcgaga
     2041 aacgtccaat acacgagcgg tagatgtttc ttgaggccac ccatacgagt gatatcctgc
     2101 tcctcatgca gagcatgaat caccgcgccg gctccgagaa acagcagtgc cttgaagaac
     2161 gcatgcgtca tgacgtgcat aacaccggac gcataggcac cgacgccgac acccatgaac
     2221 atgtacccaa gttgggatat tgtcgaatac gcgaggatct tcttgatatc gttttgcgcg
     2281 acacccatgg acgccgcgaa gagtgcggtc agcgcgccga cccacagcac aataccttgt
     2341 gaaagcggag ccagatcgta gagaaaactc gaacgggcca ccatgtacac gcccgcggtc
     2401 accatggtcg cggcatgaat cagcgcactg accggcgtcg gaccggccat cgcatctggc
     2461 aaccagacgt agagtggaat ctgtgcggat ttcccagttg cgccgaagaa caacaataac
     2521 gtaatggtgg taatcaatgc gccgccggca acgaaaacgc cccctgcagc ggcggttccg
     2581 atatcactga agctcacgct accggctgtt gtccagatga ggaacacacc aatcagaaaa
     2641 ccgaaatcac cgattcggtt gacaatgaaa gcc
//
LOCUS       DSCC01000360            1636 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1050616, whole genome shotgun sequence.
ACCESSION   DSCC01000360 DSCC01000000
VERSION     DSCC01000360.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1050616"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            12..920
                     /locus_tag="ENN56_04520"
     CDS             12..920
                     /locus_tag="ENN56_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74787.1"
                     /translation="MPWYRGNTHTHTTASDGNVSTAEAVRWYATHGYDWLVITDHNRF
                     GPTESLDLDLDKPFLTIPGSEISMRSEKLPVHLCALNPRENPAFTAMPTIIQTLQAGV
                     DRTRSLGGIPIINHPNWNWAFTDWHMAQVSDWNLLEIFNASTDCNNFGAGGRPSVEDL
                     WDSLLTRGMRVYAVAADDSHEFGVDYVGHVSLPGLGWVTVRADELSTEAILDGLQRGD
                     FYASTEISLAEYEVVDGEIRIQIAQWDKYVYTTRFIGAGGRVLSECYGVDARYRLRGD
                     EQYVRARIHSSNGGFAWTQPVFRDDR"
     gene            1298..>1636
                     /locus_tag="ENN56_04525"
     CDS             1298..>1636
                     /locus_tag="ENN56_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74788.1"
                     /translation="MKRGMLPIILLAMAVVLSSLPASAQERFPVSVGISFGRYTPSMD
                     AYNLRFVDQANPNLVDIGGGQFVTIRIRQAIDSTTTFFPQTRQEYVSGKESFLFSSAW
                     GLGLNAKVRLH"
BASE COUNT          348 a          440 c          466 g          382 t
ORIGIN      
        1 ggagtgagat aatgccttgg tatcgtggaa acacgcatac gcacaccaca gcatccgatg
       61 ggaatgtgtc cacagccgaa gcggtccgtt ggtatgcgac gcacggatac gattggctcg
      121 tcataacgga tcacaatcgc ttcggcccga cagaatcact cgatctcgat cttgacaagc
      181 cgtttctcac gattcccggg tccgaaatca gtatgcggtc ggaaaagctt ccggtgcatc
      241 tctgcgcgct gaatccgcgt gaaaatccgg cgtttaccgc catgccaaca atcatccaaa
      301 cgctgcaggc cggcgtcgac cgcacccgtt cactcggcgg tatcccgatt atcaaccacc
      361 cgaactggaa ctgggcgttc accgattggc atatggcgca ggtttccgac tggaatctgc
      421 tcgagatttt taacgcaagc accgactgca ataatttcgg agcgggcggt cgcccgagcg
      481 ttgaggatct ttgggatagt cttctcacgc gtggaatgcg agtctacgcg gtagccgccg
      541 acgattcaca cgagttcggc gtcgattatg tcggtcatgt ctcgctgccc ggcctcggct
      601 gggttaccgt tcgcgccgat gagctctcga ccgaggcgat tctcgatggg ttgcaacgag
      661 gcgatttcta cgcctcaacc gagatctcgc ttgccgagta tgaggtcgtc gacggagaga
      721 tccgcattca gatcgcacag tgggacaagt acgtgtatac gacgcgattc atcggagcgg
      781 gtggccgagt gctgtccgaa tgttacggtg tcgatgcccg ttaccgcctc cgaggtgacg
      841 agcagtacgt gcgtgcgcgt atacactcgt cgaacggggg cttcgcgtgg acgcagccgg
      901 tgttccgcga cgataggtag aacataaaaa cacgttgtcg gacgacggtt ttcgaagaaa
      961 aaccaaaatc gttgactgtg taccttgacg acggcaccga ttaccttcaa ccttggtacg
     1021 gtcacttatt ggggaagtgt cccccgcgtt ttccccccaa cgcggaatac cccgatcgga
     1081 aatccgtcct gaaaccggtg acacgaaaac cgtaaatatg ggttcgtgta tgtgatggtt
     1141 tgcttgacag aagtcaagtg cggtcgtact cttggtgggt gttccgtata ctgtcattgc
     1201 cgttcggaaa aaccgtatct tatgtcgcgt atacatccgt tcggggccga aacggagttg
     1261 agttctgagc tcgaagactg ttgaggaggg agccacgatg aaacgtggta tgctgccaat
     1321 cattctgctg gcgatggctg ttgttctcag ttcgttgccc gcatccgcgc aggagcgatt
     1381 cccggtaagc gttggtattt cgtttggccg gtacactccg tcgatggacg cctacaatct
     1441 ccgtttcgtc gaccaggcga acccaaattt ggtggatatc ggaggcggcc aattcgtaac
     1501 gattcgcatt cgtcaagcga tcgactcgac gacgacgttc ttcccacaaa cgcgtcagga
     1561 gtacgtttcc ggtaaggaat cgttcctctt ctcatcggcg tggggacttg gtctgaatgc
     1621 gaaagttcgt cttcac
//
LOCUS       DSCC01000361            3809 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016143, whole genome shotgun sequence.
ACCESSION   DSCC01000361 DSCC01000000
VERSION     DSCC01000361.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016143"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..181)
                     /gene="rpsK"
                     /locus_tag="ENN56_04530"
     CDS             complement(<1..181)
                     /gene="rpsK"
                     /locus_tag="ENN56_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDS74789.1"
                     /translation="MASPQAGRGRGRVRRTQKKTVESTGVAHVKATFNNTIITLADKD
                     GNVISWATAGKAGFKG"
     gene            complement(241..621)
                     /locus_tag="ENN56_04535"
     CDS             complement(241..621)
                     /locus_tag="ENN56_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDS74790.1"
                     /translation="MARIAGVDLPREKPARSALTYIFGIGESRALQILNATSIDPQKR
                     MGEMTDDEINRLRGTIESSFRVEGELRTEIEMNIRRLQDIGCYRGIRHRRHLPVRGQR
                     THTNARTRKGPRRQVAGARKKGKK"
     gene            complement(701..817)
                     /locus_tag="ENN56_04540"
     CDS             complement(701..817)
                     /locus_tag="ENN56_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDS74791.1"
                     /translation="MKVRSSVKKMCSYCKIIRRRGVVRVICSTNPTHKQRQG"
     gene            complement(882..1100)
                     /locus_tag="ENN56_04545"
     CDS             complement(882..1100)
                     /locus_tag="ENN56_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDS74792.1"
                     /translation="MPKEDAIEVEGTVTQALPNARFRVELESGHQVLAHISGKMRIHF
                     ITIKPGDKVKIELSPYDLTRGRIVYRHR"
     gene            complement(1160..1810)
                     /locus_tag="ENN56_04550"
     CDS             complement(1160..1810)
                     /locus_tag="ENN56_04550"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDS74793.1"
                     /translation="MRLILLGPPGSGKGTQGVRLADRIGVPQISTGDLFRKNLAESTA
                     LGRKAQEYMSKGQLVPDELVLDMVDDRLKAGDTAKGFILDGFPRTIPQADGLGKMLEA
                     HGTHIDHVVLIDLDDEEIVGRLSARRTCSKCGAIYHLIASPPKQANVCDKCGAVGTLE
                     QREDDVPETIRKRLVVYHDQTQPLVVYYEKAGLLRTVDGAQTPDKVAQDIVDAVGA"
     gene            complement(1827..3170)
                     /gene="secY"
                     /locus_tag="ENN56_04555"
     CDS             complement(1827..3170)
                     /gene="secY"
                     /locus_tag="ENN56_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDS74794.1"
                     /translation="MLDRFRNIFNIPELRNRIFFTFGVLIVYRIGGAIPTPGVDSRAL
                     AAAFADRGRDLFAVYDLFAGGAFKQATIFALGIMPYISASIIFQLLGSVWAPIQALQK
                     EGQEGQKKITQYTRYLTVLLSAVQALGVSQFLVSSNAALDPGLGFRMMVMLTMTTGSV
                     LIMWLGEQITERGIGNGMSLLIFIGIVARFPTAVIGQLRQMLEPDGGGVIPTLALFVL
                     MVLTTALVVLITQGTRRIPVRYASQRVVGGRVYGGQSTHLPLKVNASGVIPIIFAQSI
                     VLVPATIMQFAGNAASESYALQVVFGWFQPTSLVYWTMYSLLIIFFSYFYTAIIFNPV
                     DVADNLKRNNGFIPGVRPGRRTAEYIDRVLNRITLPGSVYLAAIAAFPFVVITLFEKT
                     VRMSVMPDFADFFGGTGLLIVVGVALDTLDKVESHLLVRHYDGFMKRGKIRSRRR"
     gene            complement(3163..3627)
                     /locus_tag="ENN56_04560"
     CDS             complement(3163..3627)
                     /locus_tag="ENN56_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDS74795.1"
                     /translation="MAGEQVTLADLKPPKGATKDRKRRGRGTGTGNGTYAGRGCKGAQ
                     SRSGYKRRVWHEGGQMPLQRRLPERGFTRIKPVVDQVVNLRDINDRTEGDRVDAAALR
                     AAGLIRYIDIPVKVLADGEITRAVTIIADKFSRSAREKIEAAGGKAEEPTSA"
     gene            complement(3635..>3809)
                     /locus_tag="ENN56_04565"
     CDS             complement(3635..>3809)
                     /locus_tag="ENN56_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDS74796.1"
                     /translation="IAVTLYRSPIGRTVHQKRIVEGLGLRKLNQTVIHEDKPDIRGMV
                     AKVPHLVRLEEVE"
BASE COUNT          790 a         1178 c         1009 g          832 t
ORIGIN      
        1 atcccttgaa gccggccttc ccggccgtcg cccacgagat cacattaccg tccttatcgg
       61 ccagcgtgat gatcgtattg ttgaatgtag ccttgacgtg cgcgacgccg gtcgattcga
      121 ccgttttttt ctgcgtccgc cgtacgcgac cgcgaccgcg tcctgcttgc ggtgatgcca
      181 tgcgttgttc tccgatatcc tattcgttca aacgtcacga atccgaacag attcgcaata
      241 ttacttctta cccttcttcc gggcgccggc gacctgacga cgtggaccct tacgagtccg
      301 tgcgttggta tgcgtgcgct gaccgcgcac cggtagatgc cgccggtgac gaatgccacg
      361 gtaacaaccg atatcctgca gacgacggat attcatttcg atttcggtcc gcagttcacc
      421 ttctacgcga aaactgctct cgatcgtccc acgaaggcga ttgatctcgt cgtcggtcat
      481 ctcgcccata cgcttctgcg gatcaatgct cgtcgcgttg agaatctgaa gcgcacgcga
      541 ttcgccgata ccgaagatgt acgtcaacgc tgaacgcgcc ggtttctcgc gcgggagatc
      601 tactccggca atacgcgcca tactacctac tcctctgtga actcgatttc tcatcgcgcg
      661 atcgacgaaa acgccaatcg taacgcgaga ctaccttccg tcagccctga cgctgcttgt
      721 gcgtcggatt cgtgctgcaa atcactcgta cgacgccacg gcgccggatg atcttgcagt
      781 atgagcacat tttctttacc gatgagcgta ctttcatctc tatctctccc gtccgggact
      841 tccgcaaata acggcgtaat cgttttgaac gcccgttcgt ctcagcgatg acgatacacg
      901 atacgcccac gggtcagatc atacggcgaa agctcaatct tcaccttgtc gcccggtttg
      961 atcgtgataa agtgaattcg catcttaccg gagatatgtg cgagcacttg atgcccgctt
     1021 tccagttcga cgcgaaatcg cgcgttgggc agcgcttgcg tcacagttcc ttcgacttca
     1081 atggcgtctt ctttaggcac tatctctctt ccactcttcg tgggttcggc gaatcggtac
     1141 gcgtcggttt taccgagcgt caggcgccga ctgcgtcgac gatatcctga gcaaccttat
     1201 ccggcgtttg cgcgccgtcg accgtacgta aaagacccgc cttctcatag tagacgacga
     1261 gcggctgtgt ctgatcatgg tacacaacca accgctttcg aatcgtctcc ggcacgtcgt
     1321 cttcgcgctg ctcgagcgtt ccgaccgcgc cgcacttgtc acatacgttc gcctgcttcg
     1381 gcggagacgc gatcagatga tagatcgcac cgcatttcga gcaggttcgc cgcgctgaaa
     1441 gccgtccgac gatctcttcg tcgtcgaggt cgatgagcac gacgtgatcg atgtgagttc
     1501 catgcgcttc gagcatcttt ccgaggccgt cggcctgcgg aatcgtccgc gggaaaccgt
     1561 cgaggataaa gcccttcgcg gtatctcccg ctttgagtcg gtcgtcaacc atatcaagga
     1621 ccaactcatc ggggacgagc tgccccttcg acatgtactc ctgcgccttt cgaccaagcg
     1681 ctgtactctc ggcgaggttc ttccgaaaca gatctcccgt agagatctgc ggtacgccga
     1741 tccggtcggc aagccgaacg ccctgcgttc cttttccgga tcccggtggc cccaatagga
     1801 tcagtcgcat gcgcttcctc ttttcgctat cgccgtctgc ttcgaatctt gccgcgcttc
     1861 atgaatccgt cgtaatggcg gaccagtagg tgcgactcga ctttatccaa tgtatcaagc
     1921 gccacgccga ccacgataag caatccggtt ccaccgaaga aatcggcaaa atccggcatc
     1981 acggacattc gcacagtctt ttcaaagagc gtaatgacaa cgaagggaaa cgctgcgatc
     2041 gcggccagat acacggaacc gggcaacgtg atccgattga gcacgcggtc gatatattcg
     2101 gccgtacgcc gacccggacg gacacccgga ataaacccgt tgttccgctt tagattgtcg
     2161 gcgacatcga ccggattgaa aatgatcgcg gtgtaaaagt aagaaaagaa gataatcagc
     2221 agcgagtaca ttgtccagta gacaagcgat gtcggctgga accagccgaa taccacctga
     2281 agcgcatagc tctcagatgc cgcgtttccg gcgaactgca tgatcgtcgc gggaaccagc
     2341 acaatggact gcgcaaagat gatcggaatc acgccggacg cgttcacctt cagcggcaag
     2401 tgcgtgctct gtccgccgta tacgcgtccg ccaacgacac gctggctcgc atatctcacc
     2461 ggaatgcgcc gcgtgccttg tgtgatcaac acgaccaaag ccgtcgtcag caccatgagc
     2521 acgaagagcg cgagcgtcgg aatgacgccg ccgccatccg gctcgagcat ctgccggagc
     2581 tgtccaatga ccgcagttgg gaatcgcgcg acgataccga tgaagatgag caagctcata
     2641 ccgtttccga tgccgcgctc ggtaatctgt tcgccgagcc acatgataag cacggaaccg
     2701 gttgtcatcg tcagcatcac catcatacgg aagcccagtc cgggatcgag cgcagcattg
     2761 ctcgagacga ggaactggct cacgccaagc gcctggaccg cggacagaag taccgtcaga
     2821 taccgcgtat actgcgtgat cttcttctgg ccttcctgtc cttctttctg aagcgcttga
     2881 atcggcgccc agacagagcc aagaagctgg aagataatcg acgcagagat gtacggcatg
     2941 ataccgagcg cgaagatcgt ggcctgcttg aacgcaccac cggcgaacaa gtcataaacg
     3001 gcgaacaaat cgcgtccccg gtcggcgaac gccgctgcca gagctcgcga atcgacgccg
     3061 ggtgtcggga tcgcgccgcc aatgcggtac acgatgagca ccccgaacgt gaagaaaatg
     3121 cggttcctca gttcgggaat gttgaagata ttgcgaaacc gatcaagcac ttgtcggctc
     3181 ctcggcttta ccgccggcgg cttcaatttt ctcacgagcc gagcggctaa acttgtcggc
     3241 gataatcgtg acggcgcggg tgatttcgcc gtcggcgagc accttcaccg gaatatctat
     3301 gtagcgaatc aatcctgccg cacgaagcgc tgccgcgtca acgcggtcgc cttcggtgcg
     3361 atcgttgata tcgcggaggt tgacaacctg atccacaacc ggcttgatac gcgtgaatcc
     3421 acgttcgggg agacgccgtt ggagcggcat ctgaccacct tcgtgccaca cgcgacgctt
     3481 atacccggac cgagactgcg cacctttgca gccacgaccg gcatacgtgc cgtttcccgt
     3541 acccgttcca cggccgcgcc gcttgcgatc cttcgttgcg cccttcggcg gcttcagatc
     3601 ggcgagtgtt acctgttctc ctgccatgat tcgtttactc cacctcttcc agacgcacga
     3661 ggtggggcac cttggccacc atccctcgaa tgtccggctt atcttcgtgg atcaccgtct
     3721 gattgagctt tcgcaaccct aagccttcga cgatccgctt ctgatgaacg gttcgcccga
     3781 tcgggcttcg gtataacgta accgcgatc
//
LOCUS       DSCC01000362            1573 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053331, whole genome shotgun sequence.
ACCESSION   DSCC01000362 DSCC01000000
VERSION     DSCC01000362.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053331"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            345..986
                     /locus_tag="ENN56_04570"
     CDS             345..986
                     /locus_tag="ENN56_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDS74797.1"
                     /translation="MSIPILTITSATAALRRGEVIAYPTETFYGLGAIVNDRHALERI
                     VALKQRDEGKPVSVILGRAETVTSLATSLTPSVVRLISLAWPGPLTVVLQARDDVDEI
                     ITGGTGTVAVRVPSHAHARILADASGGAITATSANIQGEPPPSSLEEVCAQFPSGLAG
                     IVGGESTPGGFATTLVRPVGRQLYILRSGVITESTLRSWWTGDVIPDTSASRA"
     gene            979..>1573
                     /locus_tag="ENN56_04575"
     CDS             979..>1573
                     /locus_tag="ENN56_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74798.1"
                     /translation="MPDRPVAEGIQVTFDPVVQERLDATASEIGAGVGHEPSSWKAKA
                     NDDEELTEDLAAHELWRRCADDLGYGFLSEELSETEWQRWRDSLASRERRRVRILVSD
                     PIDGSSEWNRAGWRHSPLTTAAMVVETTLPDATDNVVCAATVGAIWQERTWSLNTDSV
                     VVKPWSAQTVGKTVRLADEKRNLTTSETMAAAYFPTAR"
BASE COUNT          353 a          435 c          450 g          335 t
ORIGIN      
        1 gctactgcga tccgtctgac gtccggcgcg ccttcaacct gaagtccgtt catcgcattg
       61 ggataatcgg gtacttcgtc gattttcaag taaccgtcga ggtaacttac aagatccgat
      121 aatgcgacac tcatgcggac gttactcctc gcgtgggaac ttgctgaaaa tttctctttg
      181 gtgtgcacct ctgcgttcat ccgacgtaac gcgaaaggcc ttgcgccgga ttcgtcgggt
      241 cacgaatgtt acaacaatac gaaaataccg cgacggctga aacaggtccg aaaaaatgtg
      301 acgctgctcg acgatagacg cgtgtctttt accgagatag atatatgtca atccctatac
      361 ttaccataac atcggcaacc gctgcacttc gccgcggcga agtcattgcg tatccaacgg
      421 aaacgttcta cggactcggt gctatcgtca acgaccggca cgcgttggaa cgcatcgttg
      481 cactgaaaca acgcgacgag gggaaaccgg taagcgtcat cctcgggcga gccgagaccg
      541 taacgtcgct tgcgacgtcg ctcaccccca gcgttgttcg gctgatatcg cttgcatggc
      601 cggggccact taccgttgtt ctccaagcgc gcgatgacgt ggacgagatc ataaccggtg
      661 gaacaggtac tgttgccgta cgcgtgcctt ctcatgctca cgcgagaata cttgccgatg
      721 catccggcgg ggcaattacg gcaacgagcg cgaacataca aggtgaaccg ccaccctcat
      781 cgctcgaaga ggtctgcgcg caattcccaa gtggattggc tggtattgtc ggtggcgaga
      841 gtacaccggg tggattcgcc acgacactcg tacgacctgt gggacgccaa ctctacattc
      901 ttcgctccgg tgtcattacc gaaagcaccc tccgtagctg gtggaccgga gacgtgattc
      961 ccgatacgag tgcttcccgt gcctgataga cctgttgcag aaggtattca ggtaaccttc
     1021 gatccggttg tccaagaacg acttgacgca acggcttccg aaattggcgc aggtgtcggt
     1081 catgaacctt cgagctggaa ggcgaaggcg aacgacgacg aggaactcac agaggatctc
     1141 gccgcacacg agctgtggcg ccgatgtgcg gacgatctcg ggtatgggtt tctctcggag
     1201 gagctttccg aaacggaatg gcaacgctgg cgagattctc tcgcgtcacg ggaacggcgt
     1261 cgtgtacgca ttcttgtttc cgacccaatt gacgggtcga gtgaatggaa tcgggccggt
     1321 tggcgacact ccccgttgac caccgctgcg atggttgttg aaacgacgtt acctgatgca
     1381 accgataacg tagtatgcgc ggcgacggtc ggcgccattt ggcaggaacg gacgtggagc
     1441 cttaacactg actcggtggt ggttaagccg tggtcggcac aaacggtcgg aaaaaccgtc
     1501 cgattagctg acgaaaagcg gaatctcacg acgtctgaaa caatggcggc tgcgtatttc
     1561 ccgaccgctc gac
//
LOCUS       DSCC01000363            2086 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036377, whole genome shotgun sequence.
ACCESSION   DSCC01000363 DSCC01000000
VERSION     DSCC01000363.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036377"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="ENN56_04580"
     CDS             complement(<1..1648)
                     /locus_tag="ENN56_04580"
                     /inference="COORDINATES: protein motif:HMM:PF00639.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74799.1"
                     /translation="MLQLMRNQKIIKALMWIVIIAFVAWLGLELGYGGGPGGANPEVG
                     EVAGESIYWVTHRRQVAELRDFQRRQSNTMSDEFDIDEQVWQQTVQRELLRQAINRMK
                     ITVTAEEVADAMVMNPPQGFEQVPEFQTETGAFNYEAYREFLATMTDQRWAQITGMTF
                     QEYEAQMRYNLQAQKLRQAIEGGAWVTEAELRQAYRDQNETVKLRVIPAPVSLVDDSE
                     IEITDAEVQQYYRSHLREYAQPDRAMLRYVIIGRKPSARDSIIAQNQAWDIYQQIQQG
                     ADFADLARRYSEDESNAGTGGDLGTFARGMMVPPFEEAAFAARPGTVTEPVHTRFGWH
                     IIKVERKVTTPEDSVEAKHILVRDTEPGFETLDSLQTIAENLTAAHLQFADRAREYGL
                     SVETTPWFSHDVTYPISQIAEPLRRLVRWAFVGEVDSIATPHTTDDHIIVAQLAARRT
                     AGPRDLSEVRPQIESKLRLEKKVDAALRMLQPVAERIAQGQPMDAAVHGTDFSVVDVG
                     PFVRSQYLPEVEAGAFDGFTGAAFTLTRPGQMSGLVRIPER"
BASE COUNT          430 a          644 c          550 g          462 t
ORIGIN      
        1 cacgttcagg tatgcgcacc aagccactca tctgtccggg tcgagtcaac gtaaacgcag
       61 cgcccgtaaa tccgtcaaat gcaccggctt caacctctgg cagatactgc gaacgcacaa
      121 atgggccgac atctacaacc gagaagtccg tgccgtgaac ggccgcatcc atcggctgcc
      181 cctgcgcgat acgttcggca accggctgaa gcatccggag agccgcgtcg accttcttct
      241 cgagccgcag tttggattca atctgcggac ggacttcgct cagatcacgt ggacccgccg
      301 tacgccgcgc cgccaactga gcgacgatga tatgatcatc cgtcgtgtgc ggggtggcga
      361 tactgtccac ctcaccgacg aacgcccaac gaaccagccg tcgcagcggt tcggcaatct
      421 gcgaaatcgg ataggtcaca tcgtgggaaa accacggagt cgtctcaacc gacaagccgt
      481 attcccgcgc ccgatccgca aactgcagat gcgccgcagt aagattctcc gcgattgttt
      541 gaagactatc gagtgtctcg aagccgggtt ccgtgtcgcg aacgagaatg tgctttgctt
      601 cgacggaatc ctcaggtgtc gtcacctttc gctcaacctt gataatgtgc caaccgaagc
      661 gcgtatgcac cggctccgta acggttcccg gacgtgctgc gaacgccgct tcttcaaacg
      721 gtgggaccat cataccgcgt gcaaaggttc caaggtcacc accggtaccg gcattcgatt
      781 cgtcttcgga ataccgccgc gccaagtcgg cgaaatcagc accttgttga atctgctgat
      841 agatgtccca cgcctgattc tgcgcgataa tgctgtcacg ggcgctcggt ttccgtccta
      901 ttatgacgta ccgaagcatc gcgcggtccg gctgggcgta ttcgcggagg tgcgagcggt
      961 aatactgctg cacctcggcg tccgtgatct cgatctcaga gtcatcgaca agcgatacgg
     1021 gtgccggtat cacacggagt ttgaccgttt cgttctgatc tcgatacgcc tgccgtaatt
     1081 cagcttcggt gacccacgct ccgccctcaa tcgcctgacg caacttttgc gcttggaggt
     1141 tgtagcgcat ctgagcttcg tactcttgaa aggtcatgcc ggtgatttgc gcccaccgtt
     1201 ggtcggtcat cgtcgcgagg aattcgcggt acgcctcgta gttgaaagca cccgtctccg
     1261 tctggaactc gggaacctgc tcgaagcctt gaggcggatt catcaccatc gcatcggcga
     1321 cttcttcggc ggtgacggta atcttcatcc gattgatcgc ctgacggagc agctctcgct
     1381 gaactgtttg ctgccagacc tgttcgtcga tatcgaactc gtctgacata gtattcgact
     1441 gtcgacgttg aaagtcgcgc aactcagcga cctgacgccg atgcgtgacc cagtagatcg
     1501 attccccggc gacttcgccg acttccggat tcgcaccacc cggaccgcca ccgtagccga
     1561 gttcgagccc gagccacgcg acgaacgcga tgatgacaat ccacatgagc gctttaataa
     1621 tcttctgatt gcgcatcaat tggagcattt aacggtatcc cccgcctcgt tgccgaatcc
     1681 atggaacgat tcggcgactc acgtccatag gtgaatagac gtaacaagat acgccagagc
     1741 gattcgtcta cgcaacctaa tgcccctgct catatcgctc gtccgcgcac ttcctacgtt
     1801 accgaaacac ttctacgcgg taaccagcct gccgtagcct gtccacgatc tcctcggcgt
     1861 gagttccgcc gcgggtctcc agtgcgaatt ctaccgacgt ctcatcaagt ctaatatttg
     1921 cgaacgcgcg ttcgtgcgac actgtcaata cgttcgcttc aaggtcggca agtacttttg
     1981 agatctgcgc cagcgcgccg ggttgatcgg caattgttgc gcgtaaccga atcagtcggc
     2041 ccgtttttac cagaccgcgc tcgatgattc gggagagtat gttaac
//
LOCUS       DSCC01000364            1690 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048390, whole genome shotgun sequence.
ACCESSION   DSCC01000364 DSCC01000000
VERSION     DSCC01000364.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048390"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..511)
                     /locus_tag="ENN56_04585"
     CDS             complement(<1..511)
                     /locus_tag="ENN56_04585"
                     /inference="COORDINATES: protein motif:HMM:PF00588.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74800.1"
                     /translation="MTPTTSALANIDIVLVRPENQGNIGAVARIIQNFGMGSLRIVLP
                     RNFGAPSMQFDEEARRFAHRSENVLEQALSFPTVRDALADRTWTIAMSGRSGGERPAP
                     ASIRSVASRLCWQAQYNSGTIVFGPESDGLTERDLVECDAVVSIPQRRPEPSLNLAQA
                     VAITSSELFH"
     gene            702..>1690
                     /locus_tag="ENN56_04590"
     CDS             702..>1690
                     /locus_tag="ENN56_04590"
                     /inference="COORDINATES: protein motif:HMM:PF00111.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDS74801.1"
                     /translation="MATCRFNDDLLQITVGKTLFDHADQSEGIRVPTSCNRNGTCHEC
                     IVRVLEGATALSERTAAERFLTGEYRLACQAVVERDDVPLVVETLKRKPKILSKHSTV
                     AALWDPLTKRRDDDIYRDDTKIDSYHGAIYGLAVDVGTTTVVAQLIDLETGEQRKSVA
                     FANPQVFGGNNVLHRINFDTADPNRELQNILISQLDGEIHRFGSWQQIYETVIVANPT
                     MRDIFFGLPVETLGQKPFRSITEHEWRDGKRETTAVESMPYEVKLTMNKRGIVYGGPL
                     IASHVGADTAAGVLATRMFESDRPSMLIDMGTNTEVVIGNRERLIAASCPAGPA"
BASE COUNT          411 a          432 c          472 g          375 t
ORIGIN      
        1 cgtggaacaa ctccgacgat gtaatcgcaa ctgcctgagc aagattaagc gatggttccg
       61 gtcggcgctg tgggatagat acaacggcgt cgcattctac gaggtcgcgt tcggtaagtc
      121 cgtcagattc aggaccgaaa acgattgtgc cggaattgta ttgcgcttgc caacacaaac
      181 gtgacgcgac ggaccgaatc gacgccggtg ccggacgttc gccaccggat cggccactca
      241 tcgcaatggt ccaagttcgg tctgctaacg catcgcgaac ggtagggaaa gacagtgctt
      301 gttcgagcac gttttcagag cgatgagcga aacgtcgcgc ttcctcgtcg aactgcatcg
      361 aaggggctcc gaagttccgt ggaagaacga ttcgtaagct acccatcccg aaattctgaa
      421 tgatccgagc gaccgcaccg atgttaccct ggttttccgg tcgcacgagc acgatatcga
      481 tgtttgcgag agccgatgtt gtaggcgtca ttccgaatca gctccgtctc gcgaacatgg
      541 gtaacctcat ataccgatat caataggaaa acatagacaa ccgacgtagc gtagggaagg
      601 ccttaccggg tggaccggtg ttacgatatc cttatctttg cagcttccgt atttgttaaa
      661 aaacgcgtat accgtttctc tttgcgctca ggagcttcgc cttggctacg tgtcgattta
      721 acgatgattt gttgcagata acagtcggaa agacgctttt cgatcatgct gatcagagtg
      781 aaggaatccg agtaccgaca tcatgtaatc gcaacggtac gtgtcacgaa tgtatcgttc
      841 gtgtgctgga gggtgcgact gcgttgagcg agcgaacggc cgccgaacgg ttccttactg
      901 gggaataccg gttagcctgt caagcggtgg ttgaacgtga cgatgttccg ctcgtcgtcg
      961 agacgctgaa acgcaagccg aaaatccttt caaagcattc aacagttgcc gcgctttggg
     1021 acccgcttac gaagcgtcgc gatgacgata tctaccgcga tgacacaaag atcgatagct
     1081 atcacggagc gatctatggg cttgcagtcg acgtcgggac aacaaccgtt gttgcgcaat
     1141 tgattgatct ggaaaccggc gagcagcgta aatcggtagc tttcgcgaat ccacaggtat
     1201 tcggcggaaa taacgttctc caccgaatca acttcgatac ggcagacccc aatcgcgaac
     1261 tccagaacat tctgatttca caactcgacg gcgagatcca tcgctttggt tcgtggcaac
     1321 agatttacga aaccgtgatt gtcgccaatc cgacgatgcg cgacatcttt ttcggtctac
     1381 cggttgagac gcttgggcaa aagccgtttc gatcgattac tgagcatgaa tggagagacg
     1441 gtaagcgaga aacgacagct gttgaatcga tgccgtacga agtcaaactc acgatgaaca
     1501 agcgcggtat cgtatacggc ggaccgttga tcgcaagtca cgtcggagcg gataccgcgg
     1561 cgggcgtcct tgcgacgcga atgttcgaat cggaccgtcc atcgatgctc atcgacatgg
     1621 gaacgaatac cgaggttgtt atcggaaacc gggagcggct catagcggca tcgtgtcccg
     1681 cgggtccggc
//
LOCUS       DSCC01000365            1827 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043587, whole genome shotgun sequence.
ACCESSION   DSCC01000365 DSCC01000000
VERSION     DSCC01000365.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043587"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(98..685)
                     /locus_tag="ENN56_04595"
     CDS             complement(98..685)
                     /locus_tag="ENN56_04595"
                     /inference="COORDINATES: protein motif:HMM:PF08534.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDS74802.1"
                     /translation="MSGIHRLITALITAFALVGSVGAVGLGPSDRVENFTLESYDGKT
                     HSLDQYRDSVATVVMFIATQCPISNAYNQRIVSLVREYQPRGFQFLGINSNRQESVVE
                     IKGHALGAGFTFPILKDEGNVIADRFGASFTPEIYVVSPDGVILYHGRIDDSRNPDRI
                     ESHDLANALDAILTGRPIDPAETRAFGCTIKRVEN"
     gene            1011..1601
                     /locus_tag="ENN56_04600"
     CDS             1011..1601
                     /locus_tag="ENN56_04600"
                     /inference="COORDINATES: protein motif:HMM:PF03922.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74803.1"
                     /translation="MRERFYLEVVMKHIVVAALAAILGFTASTADAGPLTQGGVSLSV
                     IPQIAFPVNDARDRMENWGGGALLSTDYALKDNLALEVNLGGLFFSGIDGEKNNTPWT
                     FQDWYVYTATVGAKYYPVKEAKQPPFIGAEVGYYRWDGNGSDSAGGGKNKISIAPFIG
                     AGTTVGPIGVEGRLGYTMMADFQLIQLSVGLQLLNF"
BASE COUNT          404 a          500 c          505 g          418 t
ORIGIN      
        1 cgacgaatac gacggtcaac gtgagcagac gacgacggtt catgtggctt cctcactgtt
       61 cgtgtgcgtg tttcccgtgt ggcgtacgcg attccgatca gttctcgacg cgtttgattg
      121 tgcagccgaa cgcgcgggtc tccgccggat cgattggtcg accggtgaga atcgcgtcaa
      181 gtgcgttcgc aagatcatga ctctcgattc tgtcgggatt tcgactgtca tcgatgcgtc
      241 catgatagag aatgacgcca tccggcgaca caacgtagat ctccggtgtg aacgacgctc
      301 caaaacggtc ggcgatcacg ttgccttcat ccttgagaat cgggaacgtg aatcccgcac
      361 cgagtgcgtg ccctttgatt tcgacgacgg actcctgccg attcgaattg atgccgagaa
      421 actggaatcc gcgcggctga tactcgcgca caaggctgac tatacgctga ttgtaggcgt
      481 ttgatatggg acattgcgtt gcgatgaaca tgaccaccgt cgctacgctg tcgcggtact
      541 gatcgagtga atgcgtcttg ccgtcgtagc tttccaacgt gaagttctcg acccgatcgc
      601 tcggaccaag cccaaccgca ccgacactgc cgacaagagc gaacgcagta atcagcgccg
      661 tgatgagccg atgtatgccg ctcataagtt cctcccggac cgtttaccgg cgtctaccca
      721 acgttggttt caacgcctca atgggatcgg ttggttccct cgttctgtta acgataaccg
      781 actgcgacac gcgagaggaa gggcagtcac acaaagaatc atttgtatgt aacacgggag
      841 aaaacaacag gtacgcttga caacgaacgc ttctgttggt accattccca ctgaattgcc
      901 gcgcgaagtt atgcgagttg cgcgaagtgt tccacatctc gatctggtgg tagtaccagc
      961 gttcgtacag cgtgtgtaaa ctccgtcgaa gcaccgctat tcatgggaat atgcgggaac
     1021 gtttttacct ggaggtagtc atgaaacaca tcgttgttgc tgcgctcgca gctatcttgg
     1081 gctttaccgc ctcgactgcg gatgccggtc cgctgacaca gggcggcgta tctctgtcag
     1141 tcatcccaca gatcgcgttt cccgtcaatg acgcgcgcga ccgcatggaa aattggggcg
     1201 ggggcgcact cctctccacg gattacgcac tcaaggacaa cctcgccctc gaagtaaacc
     1261 ttggcgggct gttcttcagt gggatcgacg gtgaaaagaa taatacgcca tggacgttcc
     1321 aggactggta cgtctacacg gcgaccgttg gcgcgaaata ctaccccgta aaagaagcga
     1381 agcagccacc gttcatcggc gcggaagtcg gctactatcg ttgggacggc aacggttccg
     1441 atagcgcggg cggcgggaag aacaagatct cgatcgctcc gttcatcggt gcgggaacga
     1501 ctgttggacc catcggtgtt gaaggtcgac tcggttacac gatgatggcg gacttccagc
     1561 tcattcaact cagcgtcggg ctgcagcttc tgaatttctg atcaactgaa tacggataaa
     1621 tgagaaaggc gcgcggtaca cgatgtactg tgcgcctttt ctgctcagtt cggtgccgta
     1681 atgaatcgat gcgcaccatt cacgagtttc tcccgactgt aacacgcgag taaacaatag
     1741 gtacgcttga ctctgcgcgc tttcgtcact accattccca tcgaattact gcgcgaagtg
     1801 ttccacatct cgatctggtg gtagtac
//
LOCUS       DSCC01000366            3096 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021394, whole genome shotgun sequence.
ACCESSION   DSCC01000366 DSCC01000000
VERSION     DSCC01000366.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021394"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..697)
                     /locus_tag="ENN56_04605"
     CDS             complement(<1..697)
                     /locus_tag="ENN56_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74804.1"
                     /translation="MIDRDSIPRPPELIRRIPVTANQVFLALVLFNLFAMTGDVAIAH
                     AFNEFAFDTQYMPFFVGGFAALSTLILIPREHSTWRRALFILGMWLTVFLGVIGFWWH
                     LESQVSWRGWFSLKTYVYTAPLVAPLAYTGVAFIGLVVIKRNGHMFGVEARRWLYALI
                     AGGSFGNASLSILDHARNGFIHPAEWVPIPVTIFAGVAFLWVAFRPRLTGVVKGTLWF
                     AIVLQIIVGTIGWL"
     gene            complement(699..1949)
                     /locus_tag="ENN56_04610"
     CDS             complement(699..1949)
                     /locus_tag="ENN56_04610"
                     /inference="COORDINATES: protein motif:HMM:PF13447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74805.1"
                     /translation="MQKWGIFVVAIAVAVFGTATVWFVQQLRPEGEINRATANAVAAS
                     GECAECHARVTPAIVRQHVDGKHNAANITCFDCHGATTEMDSTHTFDHRGYRITLDVT
                     SGACERCHETQYRQFVRSRHGAPAWAAVHGPRDFTAEQIAFAETYHPGTINRPANALA
                     MQEGLAAQAAGCEMCHSVGRPNHDGSIGKCTVCHARHEFSLAGARISETCGGCHMGPD
                     HSQIEIWTASKHGVTFNIRRDRQNLNAPSNSLTAWDMDAPTCATCHMSGLGTAAVTHN
                     VGERLTYYLFDAVSEQRPNYLVNKDRMQDICLNCHAITHVERHYERAEATLHATNAKV
                     GFAQAVYDSLVAEELLTPGPFNDVADYVMYDLWHYYGRTAKHGAYMGGGDFVQWHGNY
                     EIEVKTTELLEIAKNLRREASRSR"
     gene            complement(2022..2831)
                     /locus_tag="ENN56_04615"
     CDS             complement(2022..2831)
                     /locus_tag="ENN56_04615"
                     /inference="COORDINATES: protein motif:HMM:PF01887.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74806.1"
                     /translation="MGNDPIITLTTDFGTRDGYVGAMIGVIVRQCPTARIFTITHDVK
                     PQDIIGGAWALRNAWRWFPRETIHVAVVDPGVGTDRRPILVRAGGHTFVGPDNGLITG
                     AIAGTMIDVARVIENPGARAPETSATFHGRDVFAWAAGWLTCGNEWPAIGGPIRPHHL
                     VRPPDESPRVRKDQHGTSIQGRALLADRFGNLITSIFGTLIDEAMGPRSIPEVRVNGT
                     PVRFGRTFGDVGDGESIAYVGSGGQLEIAINGGSAVEIFGNAASVVVSVPA"
     gene            complement(2892..>3096)
                     /locus_tag="ENN56_04620"
     CDS             complement(2892..>3096)
                     /locus_tag="ENN56_04620"
                     /inference="COORDINATES: protein motif:HMM:PF00294.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDS74807.1"
                     /translation="VVNTVGCGDAFVAGWMAARVGGLSAEEQLREALSVAAANAMTSG
                     AGIIEGEHAEQMRKIAVVERIRG"
BASE COUNT          603 a         1004 c          867 g          622 t
ORIGIN      
        1 gcaaccagcc gattgtaccg acgatgatct gtaacacgat cgcgaaccat agcgtgccct
       61 ttacgacgcc ggtgagtcgc gggcgaaacg ccacccacag aaacgccaca cccgcgaaga
      121 tcgttacggg aatcggcacc cattccgccg gatggatgaa cccgttgcgt gcgtgatcga
      181 gaatcgaaag cgacgcattc ccgaagctcc cgcctgcgat gagcgcatac agccagcggc
      241 gcgcttccac tccgaacatg tgcccgttgc gcttgatcac gacgagccca atgaacgcga
      301 ctccggtgta ggcgagcggc gccaccaacg gcgcggtata cacgtacgtc ttcagcgaga
      361 accagccgcg ccagctaacc tggctctcaa ggtgccacca gaagccgatc actccgagaa
      421 agaccgtcag ccacatcccg agaatgaaca gcgcgcgacg ccacgtgctg tgctcacgag
      481 gaatcaggat aagcgtcgaa agcgccgcga atccgccgac gaaaaacggc atgtactgcg
      541 tatcgaaggc gaattcgttg aacgcgtgcg cgattgctac atcaccggtc atcgcgaaca
      601 gattgaacaa aacgagtgcg agaaacacct gattcgccgt caccggaatc cgacgaatga
      661 gttccggtgg acgcggaata gagtcgcgat cgatcatatc aacggctccg gctcgcttcg
      721 cgtctgagat tcttcgcgat ttcgagcagc tccgtcgtct tcacctcgat ctcgtagttt
      781 ccgtgccact ggacgaagtc gccgccaccc atatacgcac cgtgcttcgc cgtgcggccg
      841 tagtagtgcc acaggtcgta catcacatag tccgcgacat cgttgaacgg tcccggtgtc
      901 agaagctctt ccgcgacgag cgaatcgtat accgcttgag cgaatccgac ctttgcgttt
      961 gttgcgtgaa gtgtcgcttc ggctctctcg taatgccgct cgacgtgggt aatcgcgtgg
     1021 cagttcaagc agatatcctg catgcggtcc ttgtttacga gataattcgg ccgctgttcc
     1081 gatacggcgt cgaagaggta gtacgtgagc cgttcgccga cgttgtgcgt caccgccgca
     1141 gtaccgaggc cggacatgtg acacgtcgcg caggtcggcg cgtccatatc ccacgcggta
     1201 aggctgttcg atggcgcgtt gaggttctgc cgatcgcgac ggatgttgaa cgtcacgccg
     1261 tgcttggaag cggtccagat ctcgatctgg ctgtggtccg gacccatgtg gcagccaccg
     1321 caggtctcag agattcgtgc gccggcgagg ctgaactcgt ggcgagcgtg acatacggta
     1381 cacttgccga tgcttccgtc gtggttcggg cggccaaccg agtggcacat ctcgcacccg
     1441 gcggcttgcg cggcgagccc ctcctgcata gcgagggcgt tcgccggtcg attgatcgtg
     1501 cccgggtgat acgtctcggc aaaagctatc tgctcggctg tgaagtcgcg cggtccgtga
     1561 accgccgccc acgccggcgc gccatgtcga ctgcggacga actgccggta ttgcgtttcg
     1621 tgacaccgtt cacacgcgcc actcgtaaca tcgagtgtaa tgcggtatcc ccggtgatcg
     1681 aacgtgtgcg tgctatccat ttcggttgtc gcgccgtgac aatcgaaaca cgtgatgttc
     1741 gccgcgttgt gcttcccatc gacgtgctgg cggacgatag ccggcgtaac acgggcgtgg
     1801 cattccgcac actctcccga tgcagctacc gcatttgccg ttgcgcggtt tatctcacct
     1861 tccggccgaa gctgttgaac gaaccagacc gtcgccgtgc caaatacagc gacggcgatc
     1921 gcgacgacaa atatgcccca tttctgcatc gtttgacccc ttcatccgga acgatttcgt
     1981 cagaaaaacc gtcgtatccg aaatacacac tcagacaggt atcacgcggg tacacttaca
     2041 acgacgctcg ccgcatttcc gaatatctct accgcgcttc cgccgttgat cgcgatttcg
     2101 agctgcccgc cgctgccgac gtaggcgatc gattcaccgt cgccgacatc tccgaacgtc
     2161 cgaccgaatc gaactggtgt tccgttgacg cgcacttccg gtatgctcct cggccccatt
     2221 gcctcgtcga tgagcgtgcc gaagatgctc gtgatgaggt tgccgaatcg atctgccaac
     2281 agcgcgcgac cctgaatcga cgtaccatgt tgatccttcc gcacgcgcgg tgactcatcc
     2341 ggcggacgca cgaggtggtg cggtcggatc ggaccaccga tcgccggcca ttcgttcccg
     2401 cacgtcaacc agcccgccgc ccacgcaaaa acgtcgcgtc cgtgaaaagt cgcgctcgtc
     2461 tccggtgcgc gggctccggg attttcgatc acccgcgcga cgtcaatcat cgttcccgcg
     2521 atagcgccgg tgatcaaacc gttgtccgga ccaacgaatg tgtgtccgcc tgcccgcacc
     2581 agtatcgggc gacggtctgt ccccacaccc gggtccacta ccgcgacatg aatcgtttcg
     2641 cggggaaacc atcgccacgc gtttcgcagc gcccatgcgc caccgatgat gtcttgtggt
     2701 ttgacgtcgt gtgtgatcgt aaatatgcgc gcagtcgggc actgccgaac gataacaccg
     2761 atcatcgcac cgacatatcc gtcgcgggtg ccgaagtccg ttgtcaaggt gataattggg
     2821 tcgtttccca tagctttcgc ctatcgaaac cggcgtaata cctccgcgaa tcgtatcgcg
     2881 gatcgctcgt ttcaaccgcg gatcctctct actaccgcga tctttcgcat ctgctctgca
     2941 tgctcgccct cgatgatccc tgcgccgctg gtcatggcat tcgctgcggc gacactgagc
     3001 gcttcccgca gttgctcctc agcactcaaa cctccgactc gcgccgccat ccaccccgcg
     3061 acgaacgcgt cgccgcatcc gacggtgttc acgacc
//
LOCUS       DSCC01000367            2080 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036506, whole genome shotgun sequence.
ACCESSION   DSCC01000367 DSCC01000000
VERSION     DSCC01000367.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036506"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
BASE COUNT          441 a          639 c          560 g          440 t
ORIGIN      
        1 atttaagata caatatatac tgcctttgaa attccgcaag tgatgtacgt cacttcgtat
       61 ctcgcgttat cattcgacaa cgtcctggag aatcggtcgc aggccttcat ccagatcgag
      121 cgccgcctgt cgagccgcac gcgccgaatc ggtgtcattg agcaactggt agctgatcat
      181 catattcacc cagtaacccg gctcctgcga atttagactc accgcccgac ggaaatcgtc
      241 caccgcgccg taccagtgat tttccgcttc ctgacgacgt gcggcgggat tatcgggcaa
      301 ataccgcacg cgaccgcctt catggttccg cgcgacacca cgtccgttgt acgcgtcggc
      361 gaaatccggt tgcatgtcga gcgcctgcgt gaacatttca atcgcgcggt tatactgcgc
      421 acgctggatg aactccgtgc cgcggacgta gagcttttcc acggtgatcg gcggttcaag
      481 cggcggcagc tccaaccgct gacggaattg ctgtagatca agttcgacga gcatctcgat
      541 gtcgtcaaca ttcggatacc gcaccgtcat cgaatcgacc gttccggcga tgtagcgctg
      601 acgcccttcc gtcaccgacc aaatgcgtac gaaatcacgc gcctgcttct cgcggaactc
      661 ctgcacaccg gcccgccacg catccatgaa cgtgtgaccg aacatcgtcg tttccacggg
      721 aatccacgcg tgcggacgtt gatcatccga cgcaggatcg gtccagacaa cgaactccga
      781 ttcatcaagg aacagtcccg agcggagcgc ttcgtcggga gtcaggcccg tatcgaacat
      841 catgtagacg tgaccccaaa ccgggtcgaa aacgtccagt agcgccgtcg cgatattctg
      901 cgactccaac aacgacgtat acagcacggt cacgtcgtcg cagtcaccga gccgtgagcg
      961 gaacgtatcg atcgggtaat tgatcgtgtc gaagatggtg cgcatgttcg acgtgtcgct
     1021 cacgagcttg tagtacggcg tcgtctgatc gggattgtaa ttcacgccat acttcccgac
     1081 ggcgtcgaag atgagcatcg ccgtgccgag attgcggcac cggtcgaaca cctcatcgcg
     1141 gagctgacgg aactcgttca ccgtccgatt gctaaaatcg atgacggacc gatgttccgg
     1201 cgtgatgaat gcgcaaatcc gcagtgggtt ctcccacgtc atcttgttcc gaccgagcac
     1261 gtatatcgga tcgagttgac gcgtcgagcg tttttcctca cgctcctgct gataccgcac
     1321 aaaaacctca ggctgcacga gattgtcgta attcgaggtc tcgcgcatga gcaggctgtc
     1381 gtcgagtgtc accccaacgc gtaccgtttc caccgattgc ggccgcagcg tgaactcctg
     1441 ttcgtgagca cgcccttcaa gcatcaccgt cggtacggcg atacccacgg aaaccgtcaa
     1501 cgggtccggt gatgtgtttt tcaacgtcac ctcggcgaac tccggatcgg tctcgtattt
     1561 ccggtagagt gctcgataga gcggcgtatg attcactttt gcgccgcgta tcgtgatgaa
     1621 cgaaggattg taggcgaatt gcagcgcgaa gtgattcgtc gcaccgagtg tcggatgatt
     1681 ctcgtacgcg taggagaact cgaggccgcg ccacttcaaa ccgatgccac cgctgaacag
     1741 catatcgctc ccgtacgctc cgacgccacc gatatcacgc tgccatccgc cgcgcagggc
     1801 gattatgttg tagagcgtgt actccgcgcc gagatgtaac cgatcatcga cctgcgtcgc
     1861 gagcaggagg tcgtcgccaa tacggtacga tgcgccggcg gcgtattccg tgtccttcca
     1921 attcttttcc gtgttgttgt catacgtaag gttcacgccg cccacgttcc gggcgacaaa
     1981 tccgagacgc agaccatcca acgaatgcca taccggcccc atgtctacca acgcgccgac
     2041 atccgcaccg ataccgaccg gagatccgga gcgatccgta
//
LOCUS       DSCC01000368            2080 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036504, whole genome shotgun sequence.
ACCESSION   DSCC01000368 DSCC01000000
VERSION     DSCC01000368.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036504"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1106
                     /locus_tag="ENN56_04625"
     CDS             <1..1106
                     /locus_tag="ENN56_04625"
                     /inference="COORDINATES: protein motif:HMM:PF02386.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkH"
                     /protein_id="HDS74808.1"
                     /translation="IMTYSLALVSFVQQRGSPERNEWLANIFTHDRKLPPRRMLTWIL
                     RLTIAMEIAGVIALFPVFFADYGGIKALYYSVFHSVSGFCNAGFSLFPTSLIGYQSSI
                     PMNAIMTVLIVSGAIGFVVTFEVWRWVTGKRRWFKLLVQTKLVLATYALLFAIGVVTL
                     FVFEIGGQNQEMPIGTRLLTAFFQSTLSRTSGFCTVDMSTLTYPSLFFIIVYMFIGGA
                     PGSTAGGIKVTTAAVLVLAFVSRYRSNPKTEVYNRSIPYDTIIRAVILTTVAALIVIV
                     GTFLLQITELWGMPHREASGHFFDLLFEATSAFGTVGLSTGVTPTLTSAGRIVVLVLM
                     FVGRVGPLTLAALLLGKQATSRLSYVEEDVMIG"
     gene            1111..1764
                     /locus_tag="ENN56_04630"
     CDS             1111..1764
                     /locus_tag="ENN56_04630"
                     /inference="COORDINATES: protein motif:HMM:PF02254.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDS74809.1"
                     /translation="MPSLLMIGLGSFGYHVVRTLSNSDFFVTAIDSDVAKVEAVKTYV
                     QEPMVGDATQLETLRELSIRDYDYVVVSLGDRLDTSILTCLHLHDLQAKNIVVKVNDV
                     EHARVLREFHVSRVIFPEREAAEDLAHTIADLNVLHSVAVTEGFTLVELAVPSKFEGK
                     KLSELDLPRNFGVQVVLIHQHVPEETILPRADFVLKSSDTLAIIGSDEAIRRVQNEF"
     gene            1898..>2080
                     /locus_tag="ENN56_04635"
     CDS             1898..>2080
                     /locus_tag="ENN56_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74810.1"
                     /translation="MPVSRRDFLKSGLILTGGVLLPRWAHAADASRFDRLPDVVHVTG
                     GGPDTLLQRAITELGGI"
BASE COUNT          461 a          566 c          522 g          531 t
ORIGIN      
        1 gaattatgac gtactcactc gcactcgtta gcttcgttca gcaacgtggt tcacccgaac
       61 gaaacgaatg gcttgcaaac atatttaccc acgatagaaa actcccgccg cgtcgcatgc
      121 tcacgtggat cttacgtttg acaattgcca tggaaattgc cggggttatc gcgttgtttc
      181 cggtgttctt tgcggactat ggcggaatca aagcgctgta ttactccgtc ttccattccg
      241 tgagcggatt ttgtaacgcc ggattctctc tcttcccaac aagcctcatc ggatatcaga
      301 gtagcatacc catgaacgcg atcatgactg tcctcatcgt ttcgggagcg atcgggttcg
      361 tcgtaacgtt tgaggtgtgg agatgggtaa caggtaaacg ccgctggttc aagcttctcg
      421 ttcaaacgaa gttagtcttg gcaacatatg ccttactctt tgcgattggg gtcgtaacac
      481 tttttgtatt cgagataggc ggacagaatc aggagatgcc aattggtaca cgccttctga
      541 ccgcgttctt tcaatcaaca ctatctcgca cctcgggatt ctgcaccgtc gacatgagca
      601 cactgacgta tccttcgctc ttttttataa tcgtctacat gtttatcgga ggagctccgg
      661 gttcgacggc cggcggtatt aaagtaacaa ccgcggctgt tcttgttctt gctttcgtgt
      721 cacggtaccg ctctaatccg aaaacagaag tttacaaccg gagtatacct tacgacacga
      781 ttatccgtgc agtgatcctt acgacagtag cagcgctaat cgtaatcgta ggtacatttc
      841 tccttcagat caccgaactc tgggggatgc cgcaccgcga ggcgagcggg cacttcttcg
      901 atctactctt cgaggcgacg tctgcgttcg gcactgtagg tctcagcacc ggcgtaactc
      961 cgacgttgac atccgcaggt cgcattgtcg tactcgtact gatgtttgtc ggtcgcgttg
     1021 gaccactgac actcgctgca ctcctcctcg gcaagcaagc cacttcgcgg ctctcgtatg
     1081 tcgaggaaga tgtaatgatc ggatgacatt atgccgtcct tactcatgat cggactcgga
     1141 tcgttcggat accatgtcgt ccgaacctta tccaacagcg acttcttcgt aaccgcaatc
     1201 gactcggacg tagcgaaggt cgaagcagtg aaaacgtatg tacaagaacc gatggttggc
     1261 gatgcaacgc agctggagac gcttcgtgaa ctgagcatcc gagactacga ttacgtcgtt
     1321 gtcagcctcg gagatcggct cgataccagt atcctcacgt gtctgcactt acacgatctt
     1381 caggctaaga acatcgttgt gaaggtgaac gacgtggagc atgcgcgtgt attacgagag
     1441 ttccacgttt cccgtgtgat ttttcccgag cgagaagccg ccgaggacct cgcgcacacc
     1501 atcgctgatt tgaacgtgct gcactctgtc gcggtaactg aaggattcac actcgttgaa
     1561 ttggctgtgc cctcgaagtt cgaagggaag aagctctccg aactcgatct acctcgaaac
     1621 tttggcgttc aagtcgtgct cattcaccaa cacgttcccg aagaaacgat acttccgcgc
     1681 gccgacttcg ttctcaaatc aagcgataca ctggcgatca tcggttccga cgaggcgatc
     1741 cgacgagttc aaaacgagtt ttaggagaat ccttgtagcg tcccgctacc ggaccgaaca
     1801 gaaccatctt gccgaggagt gctgagcggt gtattcttct caatagaccg atgtaatcag
     1861 tctgttcggg gttcatctcg aaaaacgagg tcctcccatg ccagtatctc gccgtgattt
     1921 cctcaagtcc ggattgattc tcacgggtgg cgtgcttctc ccacgttggg cgcacgcagc
     1981 tgacgcttca cggttcgacc gacttcccga tgttgtgcat gtcaccggtg gtggtcccga
     2041 tacactcctc caacgcgcga tcacggaact cggtggaatc
//
LOCUS       DSCC01000369            1544 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054710, whole genome shotgun sequence.
ACCESSION   DSCC01000369 DSCC01000000
VERSION     DSCC01000369.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054710"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..972)
                     /locus_tag="ENN56_04640"
     CDS             complement(<1..972)
                     /locus_tag="ENN56_04640"
                     /inference="COORDINATES: protein motif:HMM:PF00654.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDS74811.1"
                     /translation="MVLRTRWYREIARLARVSVRVTTYLGGLSPNALVILLAAIVGIC
                     SGFTAVCFTLMLEFSHDLFFGAHEWALRTDARWYWFLPLVPAIGGLIVGPIIYHFARE
                     AKGHGVPEVMDAVASHGGRIRGRIVVFKTVASAITIGSGGSAGQEGPIVQIGAALGST
                     FGRMFQLSSDRLKVLVGCGAAGGISAIFNAPIAGVLFALEVILGDFRIQTFSPVVISA
                     VLASVVAQSFLGSAPAFDIPEYTLSAPWELGNYLVLGALAGVAAVAFIRTLYSFEDFF
                     DQRVKIAPWLKPAVGGLLVGTIALVDPGVFGGGYRQIGAALHGDMLVF"
     gene            1208..>1544
                     /locus_tag="ENN56_04645"
     CDS             1208..>1544
                     /locus_tag="ENN56_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74812.1"
                     /translation="MTSRYWIRVPYIALSSGEGIDSLIDFCRASRAHGVLLFTANVDT
                     EPGLLDVDDVQKRTTRLKSVVPLFRDAGLDVHINVHSTLGHGSPTINTVDRLGFQPMI
                     DHLGQVSDTA"
BASE COUNT          398 a          462 c          401 g          283 t
ORIGIN      
        1 gaataccagc atgtcgccat ggagcgccgc accgatttgc cggtacccgc ctccgaacac
       61 tccaggatcg acgagagcga tcgtaccaac aaggaggcca ccgacagccg gcttgagcca
      121 cggtgcaatc ttgacccgct gatcaaagaa atcctcgaac gagtagagtg tgcgaatgaa
      181 tgcgacagca gcaacacctg caagggcgcc gagaacgaga tagttgccga gttcccacgg
      241 agcgctcaac gtatactcgg gtatgtcgaa cgccggcgcg gatccgagaa aggattgtgc
      301 caccaccgaa gcgagtaccg ccgagatcac gaccggactg aatgtctgga tgcggaaatc
      361 accgagaatc acttcgagtg cgaagagaac tcccgcgatc ggtgcgttga aaatcgccga
      421 tatgccaccc gccgcaccac atccgacgag taccttcaaa cggtcactcg aaagttgaaa
      481 catgcgcccg aacgtcgaac cgagcgctgc accgatttga acgatcggac cttcctgtcc
      541 cgccgaacca ccagacccga tcgtgatcgc cgatgcgacg gtcttaaaca cgacaatccg
      601 accacgtatc cttccgccat gtgacgcgac tgcgtccatt acttccggaa caccgtgtcc
      661 cttcgcttcg cgggcgaaat ggtagatgat cggcccaacg atcaatccgc cgatagccgg
      721 cacaagagga agaaaccaat accaacgtgc gtcggtacgt aatgcccatt catgcgcgcc
      781 gaaaaacaga tcgtgcgaga actcgagcat gagggtgaaa cagacggcag taaacccgga
      841 gcaaatccca acaatcgcgg caagcagaat aacaagcgcg ttcgggctca accccccgag
      901 atacgtcgtc acgcgcacac ttactcgtgc gagacgtgct atctcacgat accaccttgt
      961 ccgaagaacc atcggggtcg atccgaatga gagcagtgag cgaatacatc gctcgatact
     1021 tagcgtatag tgcgaattac actatgatag gattatgaaa ggtacccaca cccatggaaa
     1081 caccgcaacg gactaacccg ccgaaatttc gacctacggt cttcccatat tgtctctatg
     1141 ccgtatcttt ggcattcact atgaatacga cggatcagtc agaagacttc agggaggacg
     1201 accgcacatg acgagtcgct actggattcg agtcccttac atcgctcttt cgagcggcga
     1261 ggggattgac agcctgatcg atttctgtcg ggcgtctcgg gcgcacggcg ttctcctttt
     1321 caccgcgaac gtagataccg aacccggttt actggacgtg gacgacgttc aaaagcgtac
     1381 aacgcgtctt aagtcggttg ttcccctatt tcgagacgcc gggcttgacg tgcatatcaa
     1441 cgtgcacagc acactcggac acggctctcc aacgataaac acggttgatc ggctcggatt
     1501 ccagccgatg attgatcacc tcgggcaggt gtcggacacc gccc
//
LOCUS       DSCC01000370            2620 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1026753, whole genome shotgun sequence.
ACCESSION   DSCC01000370 DSCC01000000
VERSION     DSCC01000370.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1026753"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            281..1021
                     /locus_tag="ENN56_04650"
     CDS             281..1021
                     /locus_tag="ENN56_04650"
                     /inference="COORDINATES: protein motif:HMM:PF00795.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDS74813.1"
                     /translation="MKIAAIQLDSAPMEVMTNVRNAMYACRNAFLHGARMIFLHEGLT
                     ADYSPEPLRYGRALESFEVHGFTDLAKQFDGYIALGLNEVWRGQPFISCAYLGPTGVV
                     DVYRKSVLWSARNRDDCVPYQMGFRQERGILGHGDGPRVVNVGGLRIGTLICADGDVP
                     ESWDVFRKDVPDLVLHQSNQTFLRMDYRQAVAREIDRPMVVTNRVGFSYHRFQTGGTR
                     IVRRDGSIAVAANAEGREQTIFADLADL"
     gene            1041..2258
                     /locus_tag="ENN56_04655"
     CDS             1041..2258
                     /locus_tag="ENN56_04655"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74814.1"
                     /translation="MRLGPTTSQRDIFVSHQPAPRTLFRRISMTPRERYREALLFGTP
                     DKYPLSPGGPREKTRARWYQEGLPRDADYMSELYRILDIEPEIPEKPAPGLNVDFRMI
                     PQFEEKVLEHRNGHYIVQDWMGAITEISDEYDYTYIRAARDFVTRKWHRFPVQSRKDF
                     EEKIRWRYDVDAPGRFPDDFDERCEKSRDRDNVLHVHFNGPFWQLREWCGFEGLCTLM
                     IDDPEFVDDMARFWTEFVSATLDRIISRVELDSVGLSEDMAYKSFSMISPSMVRRFLK
                     PAYDIWVPAIKASGCPIIDMDSDGFIGELIPIWIEAGINVCDPIEVAAGNDIVEFRKR
                     FGTRIAYKGGIDKRAIAAGGDVMRAELQRVVPPILKDGGFIPSCDHGVPHDISWPNFI
                     EYTRLLAQMTGWL"
BASE COUNT          608 a          721 c          711 g          580 t
ORIGIN      
        1 atcggaagac tcgaaacgca acaatgatcg tgttcccgcc aaatgcgccc ctacaccccg
       61 aaatataggc gaagactcgc gtatggcaac cccctaacac cacgatattt cataaaacgt
      121 cattcataaa cgacttagct tcgaaaaacc gagtgttttc agggagatac atcgacgaat
      181 ttgccgttcc taccgtcgcc cttcctcgtt gcatcgatgc ccttgcccga agatattcac
      241 acgagattcg gaaaaacgca agaccctgga gacgtttttt atgaaaatcg ccgcgattca
      301 gctcgactct gctccgatgg aagtaatgac aaacgtacgg aacgcgatgt atgcgtgccg
      361 taacgccttt ctccacggtg cccgtatgat ctttctccac gaaggactta ccgccgacta
      421 ttcaccggaa ccactgcggt acggtcgcgc gctcgagagc ttcgaagtgc acggatttac
      481 ggatctcgcg aagcagttcg acgggtatat cgcgcttgga ttgaacgagg tctggcgcgg
      541 tcagccgttc atttcgtgcg cgtatctcgg tccgaccggt gtcgtcgatg tataccggaa
      601 gagtgttctg tggtccgcac gcaatcgaga cgattgtgtc ccgtaccaaa tggggtttcg
      661 gcaagagcgc ggcatactcg gccatggcga cggtccgcgc gtggtgaatg ttggtggcct
      721 acggatcggc acacttatct gtgcagacgg cgatgtgccg gagtcctggg acgtgttccg
      781 caaggacgta ccggacctcg tgctacacca gagtaatcag acctttcttc gaatggatta
      841 ccgtcaagca gtcgcgcgcg aaatcgaccg tccgatggtc gtcacgaatc gcgtcgggtt
      901 ctcgtaccac cgttttcaga cgggcggaac gcgaatcgtg cgacgcgatg ggagtatcgc
      961 tgtcgccgcg aatgcggaag gtcgcgagca gacaatcttc gccgatctcg cggacctctg
     1021 aaatcgcatg aatccgacca atgcggcttg ggccgacgac gtcgcagcgg gatattttcg
     1081 tatcccatca acccgcacct cgcactctgt tcaggaggat ttccatgact ccgcgtgagc
     1141 gataccgcga agcccttctc tttggcacgc ctgacaagta tcccctgagt cccggcggtc
     1201 cacgtgaaaa gactcgcgcg cgatggtacc aagaaggtct cccgcgagac gctgactata
     1261 tgtcggaact ctatcgcatc ctcgacatcg aacccgaaat ccccgagaaa cccgctcccg
     1321 gtctgaacgt cgatttccgc atgattccgc aattcgaaga gaaagtgctc gagcatcgaa
     1381 acggacacta tatcgttcag gactggatgg gtgcgattac ggaaatttcg gatgaatacg
     1441 actacacgta cattcgcgcg gcgcgagatt tcgtaactcg gaaatggcat cgcttccctg
     1501 tgcagagccg caaagatttc gaggaaaaaa tccgctggcg atacgatgtc gacgcgccgg
     1561 gacgatttcc agacgatttc gacgagagat gcgagaaatc acgagatcgc gacaacgttc
     1621 ttcacgttca cttcaacggt cccttctggc agcttcgtga gtggtgtgga tttgaaggtc
     1681 tctgcacgct tatgatcgac gacccggaat tcgtcgatga catggcgcga ttctggaccg
     1741 agttcgtatc ggcgacactg gatcggatca tcagtcgcgt cgaactggat tccgtcggtc
     1801 tttcagaaga catggcgtac aagagtttca gcatgatctc gccgagtatg gtccgccggt
     1861 tcctcaaacc cgcctacgac atctgggtac ccgccatcaa agcgagcggt tgtcccatca
     1921 tcgatatgga ttccgacgga ttcatcggag agcttatccc aatctggatc gaagccggga
     1981 tcaacgtctg cgatccaata gaggtggctg cgggaaacga tatcgtggag tttcgtaaga
     2041 gattcggaac gcgtatcgcc tataaaggtg gaatcgacaa gcgcgcgatc gcagccggag
     2101 gcgatgtgat gcgtgccgaa ctgcagcgcg tcgtgccgcc aatacttaaa gacggcggat
     2161 tcatcccaag ttgcgatcat ggtgtaccgc acgatatctc atggccgaac ttcatcgaat
     2221 acacgcggtt acttgcccag atgaccggtt ggctctgaga tgggctatca agtgaaacac
     2281 gcgcgaccaa gcacgttctc ggtcgcgcgt gtttcatgtc tcctcagcgc atcggtacta
     2341 tatttcgaat cgcactccga accgatagaa acgcggactc atgaattgca gccagtcaga
     2401 ttccggactg atcggtcgcg accacaacgg cgaattcgga tcgattttgt atggctgcgt
     2461 gagaatgtag aacgtcgact cctcgccgac tttgatatcg tcacccagac tccgtcgagt
     2521 ataaacgtag ttgaggtaat cctgccagtt ctttacaccg gccgaggtca agtgcttcgt
     2581 gttgagtacg ttgagaacgt cgagatagac tgacatcgac
//
LOCUS       DSCC01000371            2509 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028351, whole genome shotgun sequence.
ACCESSION   DSCC01000371 DSCC01000000
VERSION     DSCC01000371.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028351"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..369
                     /gene="tsaE"
                     /locus_tag="ENN56_04660"
     CDS             <1..369
                     /gene="tsaE"
                     /locus_tag="ENN56_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDS74815.1"
                     /translation="GSLGAGKTTLVQGICHGLGVTAWANSPTFTLINEYVGTRNGEPL
                     RVYHCDFYRIDDPEELATLALDEVFYGDGVTLVEWPDVAEEWAPDDAVRVKITRIGAN
                     ERRLLATLEDVGRRRSASSP"
     gene            296..1510
                     /locus_tag="ENN56_04665"
     CDS             296..1510
                     /locus_tag="ENN56_04665"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcobamide--CoM methyltransferase"
                     /protein_id="HDS74816.1"
                     /translation="MARTNGVSSLRSKTWGEDAPRLRLNELEARFEFAPFPRLTFRSF
                     LSYEHFRSHPSKGMHMSNDIVWAPLPREEVVKAVERRNPVRPPLVMAKWWGEGLGEQY
                     GERLRYFDRYPDDVAMFMISPLDAGNMNLSWQIEGGKAHDSKVVINDWAQLDEFIEKI
                     PDPRNDPALVDIVQRAERARAEDRYVLFGWWGLFFERPWGLRGMENLMVDYYTAPDMV
                     HRLHEALTQHYLGYIRHAADVIQPDGFWTSDDLGHQTSSMMRPSTFDELHKPYYTRIG
                     SELHERGIHFWLHSCGNNTPLMPALIDAGVDIFHPVQKHTMDERSIADEFGGRISFLA
                     GIDVQHTLQEMDSEGVRAEVRFLIDTFDRPDGGMAIAAGNGIVNGTPFENIDAFLDEA
                     LRYGTEHRQRFQ"
     gene            complement(1576..2445)
                     /locus_tag="ENN56_04670"
     CDS             complement(1576..2445)
                     /locus_tag="ENN56_04670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDS74817.1"
                     /translation="MGWFDKWKTGLGAGKKPVPDGVWEKCEGKGADSHLGCGRTLYRK
                     DLAANSWVCPSCDFHHRMAARDYFTLLLDNGEYETLAENVASADPLKFKADKRYADQI
                     KSYAKKTNTTAAFIAGYGTIDGRPLALGAMDFDFAGGSLGSAEGERIRRLIDFAQDRR
                     WPLVIISKSGGARMQEASLSLMQMAKTSARLAEFSEAGLPYISLLTSPTTGGVTASFA
                     MLGDVIIAEPKALIGFAGARVRDTIKEELPEGFQRSEFLLAHGFVDRVVDRRNLRDEI
                     CRLTRVLAPAAVA"
BASE COUNT          495 a          786 c          723 g          505 t
ORIGIN      
        1 ggaagtctcg gtgcgggaaa aacgacgctt gtgcagggaa tctgccacgg gctcggggtc
       61 accgcgtggg cgaacagtcc gacattcacg ctcatcaacg agtacgtcgg cacgcgaaac
      121 ggcgagccac tccgcgtcta tcactgcgat ttctaccgca tcgacgaccc cgaagagctt
      181 gcgacacttg cgctcgacga ggtgttctac ggcgatggtg tgactctcgt tgaatggccc
      241 gatgtcgctg aagaatgggc gccggatgat gccgtgcggg tgaagatcac gcgtattggc
      301 gcgaacgaac ggcgtctcct cgctacgctc gaagacgtgg ggcgaagacg ctccgcgtct
      361 tcgccttaac gaactcgaag ctcgcttcga gttcgctccc ttcccccgtc tcaccttccg
      421 ctcgttcctt tcgtacgaac acttccgcag ccacccgtca aaaggaatgc atatgtcaaa
      481 cgatatcgtg tgggcgccac tgcctcgtga agaggtcgtg aaggctgttg agcggcggaa
      541 tcccgtccga cctccgctcg tgatggcgaa atggtggggc gaagggctcg gcgaacaata
      601 cggcgagcgg ctccgctact tcgaccgcta ccccgacgat gtcgcgatgt tcatgatctc
      661 gccgctcgac gcgggcaata tgaatctctc gtggcaaatc gaaggcggga aggcacacga
      721 cagtaaagtc gtcatcaacg actgggcgca gctcgacgag ttcatcgaga agattcccga
      781 tccccggaac gacccggcgc tcgtcgacat cgttcagcgc gccgaacgag cgcgcgccga
      841 ggaccgatac gtgttattcg gctggtgggg cttgtttttc gagcggccgt ggggactccg
      901 cgggatggaa aacctcatgg tcgattacta caccgcgccc gacatggtgc atcgcctgca
      961 cgaagcgctt acccaacact acctcggcta cattcgtcac gccgcggacg tcattcagcc
     1021 cgacggattc tggaccagcg acgacctcgg tcaccagacc tccagcatga tgcgcccgag
     1081 cacgtttgat gaactccaca agccgtatta cacgcgcatt ggtagcgaac tccacgagcg
     1141 cggtatccac ttctggctcc actcgtgcgg gaacaacacg ccgctcatgc ccgcgctcat
     1201 cgatgcgggc gtcgacatct tccatccggt gcagaaacac accatggatg aacgctcgat
     1261 cgcagacgag ttcggcggtc ggatcagttt tctcgcggga atcgacgtgc agcacacgct
     1321 tcaggaaatg gattcggagg gcgttcgcgc cgaggtccgg ttcctcatcg acacgttcga
     1381 tcggcctgac gggggtatgg caatcgccgc gggtaacggc atcgtcaacg gaacgccgtt
     1441 tgagaatatc gacgcgttcc tcgatgaagc gcttcggtat ggcacggagc accggcagcg
     1501 tttccagtag actacgacgg cgtcgtaacc tccacccata cgcatttcgt tatttcgcga
     1561 aaagacgcta tactgctacg caaccgccgc cggggcgaga actctcgtga gtcggcagat
     1621 ttcgtcgcgg aggttccgtc ggtcgacgac gcggtcgacg aaaccgtgtg caaggagaaa
     1681 ttcgctccgc tggaatcctt ccggcagttc ctccttgatc gtatcgcgga cgcgcgcgcc
     1741 ggcgaaccca atgagtgcct ttggctcggc gataatcacg tcaccaagca tggcgaaact
     1801 cgccgtgaca ccaccggtgg ttgggcttgt cagcagactg atgtaaggca atcccgcctc
     1861 ggagaactcc gcgaggcgag cgctcgtttt cgccatctgc ataagcgaga gcgacgcttc
     1921 ctgcattcgc gcgccacctg acttgctgat gatcaccagc ggccaacggc gatcctgcgc
     1981 gaagtcgatg agtcggcgga ttcgctcgcc ttctgccgaa ccgaggctgc cgcctgcgaa
     2041 atcgaaatcc atcgctccga gcgcaagcgg acgaccgtca atcgtgccgt atcccgcgat
     2101 gaacgcggcg gtcgtgtttg ttttcttcgc gtacgacttg atctggtcgg cgtagcgttt
     2161 gtccgccttg aatttgagcg ggtccgccga tgcgacattt tccgcgagcg tttcatactc
     2221 gccgttgtcg agaaggagcg tgaaatagtc ccgggcggcc atgcgatgat ggaaatcgca
     2281 acttgggcac acccacgagt tcgccgccaa gtccttccgg tagagcgtcc gcccacaacc
     2341 gagatgcgag tccgcgccct tcccttcgca tttctcccag accccgtccg gcaccggttt
     2401 cttgcccgct cccagtccgg ttttccattt atcgaaccat cccatagtcc gcctcatgtt
     2461 tctcgttcga cgcgacaatc ttcaactttc gatactcgtc gcgaaatca
//
LOCUS       DSCC01000372            2894 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023471, whole genome shotgun sequence.
ACCESSION   DSCC01000372 DSCC01000000
VERSION     DSCC01000372.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023471"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..1123
                     /locus_tag="ENN56_04675"
     CDS             <1..1123
                     /locus_tag="ENN56_04675"
                     /inference="COORDINATES: protein motif:HMM:PF13727.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74818.1"
                     /translation="VEGLSGAFMCVRRTVIDQVGGLDEEFFMYGEDLDWSYRITEAGW
                     KLAYVPQAEIVHFKGESARTMPRLRQLYEFHRAMVIFVRKHIAKTRNPLSVALIEAGI
                     VLRGIGVTLWRFFLAILRPLLDVSLLAVAVVLALATRSLSGWTVPPFTGNEWALISFV
                     FIVSGIAGAAVTGLYRKQPGAMERTDAGRAILAAVVGAAICIVAMFFIKTINFSRIVT
                     GLAWMYAGGLAAAWRWIVAPSGNRPRGRGIVVGCGARANAFLEEFVKPARNYHVMGVV
                     RGTDDDPECSSVAGYPVIGDLSDLPILLRRLKVDDVIVAWEHFRYADVLGFVRRGGGY
                     PQRVRLVPDGLDLNGAAQTTASEGDWPLIDVKLPKRSIL"
     gene            1120..2733
                     /locus_tag="ENN56_04680"
     CDS             1120..2733
                     /locus_tag="ENN56_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDS74819.1"
                     /translation="MTPDASHRSRTRRRLVWIIVVLAIISFVAPKVWASARDFYTKLQ
                     VLSNIVSLARDVYVDEIDPGKLMDGAINGLFDELDPHSSYLAKDEAQRFTEKVQGSFG
                     GVGIQYSVVEGVPTVIATIEGGPAEYAGVRAGDQIVRVDGKTTIGWRSDDVQTHLRGP
                     KGSRVSIAVSRAGVDEPIEIDIVRGDIPLLSIPYAFMLNDSAAYVRITNFAATTGREF
                     ATAMDSLDRIGMRYLVIDLRNNGGGDMDGAVAIANHFLSDGTTIVSQRGRWEPANQII
                     YASGGARKYDVPVVVLINHGSASASEIVAGALQDTDRGIIVGQRSFGKGLVQRSFDLS
                     SRVDGGGIVLLTVARYYTPTGRLIQRDYSAGTAQYILDAMADGSSVDTTAQPAFATPL
                     GRIVYGGGGISPDYHTEAQSVDRAIVRLANRSAFFRFADDLARRNRVIAETSEAYRVL
                     EADDSLWSEFVDFAMASDTTFTDVDLERIRPDVTPYVLAGIAGRFQGPEARYRLLVPI
                     DAELAEALHHLNDAAELLRAGNARESRNE"
BASE COUNT          559 a          786 c          910 g          639 t
ORIGIN      
        1 ggttgaaggc ctatccggtg cattcatgtg cgtgcgtcga acggttatcg atcaggtcgg
       61 tggtttggac gaagagtttt tcatgtacgg cgaggatctc gactggtcct accggatcac
      121 cgaagcgggt tggaaactgg cgtacgtccc gcaggcggaa atcgttcatt tcaagggcga
      181 gagcgcccgg acgatgccga ggttacggca actttacgag tttcatcgcg cgatggtaat
      241 cttcgtacgg aagcatatcg ccaagacgcg caatccacta tccgtcgcgc tgatcgaggc
      301 cggtatcgta ttgcgcggca tcggtgtaac gctctggcgc ttctttctcg ccatactgcg
      361 accactcctt gatgtgtcgc tcttggctgt cgcagtggtt cttgcgcttg cgactcgctc
      421 gctgagcggt tggacggttc cgccgttcac cgggaacgag tgggcgttga tttcgttcgt
      481 tttcatcgtt tcgggaattg ccggcgcagc ggtaacgggg ctgtaccgca aacaacccgg
      541 agccatggaa cgcaccgatg ccggtcgcgc gatactcgcg gcagtggtcg gcgcggcgat
      601 ctgcattgtc gccatgttct tcatcaagac gatcaacttt tcccgtatcg tgactggtct
      661 ggcgtggatg tatgcgggag ggcttgcggc ggcgtggcga tggatcgtgg caccgagcgg
      721 gaatcgtccg cgcgggcgtg gcatcgtcgt cggttgtggc gcgcgggcga acgcgtttct
      781 tgaagagttc gtcaagccgg cgcggaatta ccacgttatg ggcgttgtcc ggggcaccga
      841 tgacgatccg gagtgctcgt cggtcgcagg atatccagtg atcggcgacc tttccgatct
      901 gccgattctg cttcgtcgat tgaaggttga cgacgtgatt gtcgcgtggg aacactttcg
      961 ttatgccgat gtgcttgggt ttgtccgtcg aggcggtggg tatccgcagc gcgtacgcct
     1021 tgttcccgac ggtctcgacc tcaacggtgc ggcgcagacg acggcatctg aaggcgactg
     1081 gccgcttatc gatgtgaagc taccgaagcg gagcattcta tgacgccgga cgcttcacat
     1141 cgatctcgca cgcgacgacg tcttgtgtgg ataatcgtcg tgcttgcgat cattagcttc
     1201 gttgccccca aggtgtgggc ctcggcgcgc gatttttaca cgaaactcca agtactgagt
     1261 aatattgtaa gtcttgctcg cgacgtctat gtcgacgaga tcgaccccgg taagctcatg
     1321 gacggcgcta ttaacggcct cttcgacgag cttgatcccc acagcagtta cctcgcgaag
     1381 gatgaagcgc agcgatttac ggagaaggtt caaggtagct tcggtggcgt ggggattcag
     1441 tactcggtcg tcgagggagt gccgacggtc atcgcgacga ttgaaggcgg acctgccgaa
     1501 tacgcgggcg tgcgcgccgg cgatcagatc gtgcgcgttg acgggaaaac aacgattggg
     1561 tggcgttctg atgacgttca aacgcatcta cgcggaccga aaggctcgcg agtatcgatc
     1621 gccgtgagtc gtgccggtgt cgacgaacca attgagatcg acatcgtacg cggtgatatc
     1681 ccgcttttga gtattcctta cgccttcatg ctcaacgatt cggctgcgta cgtccggatt
     1741 acgaattttg cagccacaac cggacgcgag ttcgccaccg caatggattc gctcgatcga
     1801 atcggaatgc ggtacctcgt catcgatctg cggaataacg gtggcggcga catggatggc
     1861 gcggtcgcga ttgcgaacca cttcctgagt gacggaacga ctatcgtatc gcaacgcggt
     1921 cgttgggaac cggcgaatca gattatctac gcatcgggcg gtgctcgaaa atacgatgta
     1981 ccggtcgtag ttctgatcaa ccacggctcc gcgagcgcgt cggaaatcgt cgcgggtgcc
     2041 ctccaggata ccgatcgcgg catcatcgtt ggtcagcgaa gctttggaaa aggactcgtg
     2101 caacggtcgt ttgatctcag tagccgagtc gatggtggtg gaatcgtcct attgacggtt
     2161 gcgcggtact atacgccgac gggacgtctg attcaacggg actacagcgc cggcaccgcg
     2221 cagtacatcc tggacgcgat ggccgacggt tcgtcagtgg atacgaccgc tcaaccggcg
     2281 ttcgcgacac cgctcgggcg cattgtttac ggtggtggcg gcatttcccc cgattaccat
     2341 accgaagcgc aatcggtgga tcgcgcgatt gttcggcttg ccaaccgctc ggcatttttc
     2401 cgtttcgcgg atgacttggc gcgtcgaaat cgggtaatcg cggagacgtc ggaagcgtat
     2461 cgggtacttg aagccgacga cagcctgtgg tcggagttcg tggacttcgc gatggcgtcc
     2521 gacacaacct tcaccgacgt cgatcttgaa cgcatcagac ctgatgtaac accgtatgtg
     2581 ctcgctggaa tcgcgggtcg tttccaaggc cccgaagcac ggtaccgact gttggtgccg
     2641 atcgatgcgg agcttgccga ggcgctacac cacctcaacg acgcggcgga gttgttacgc
     2701 gccggtaatg cacgggagag ccgaaatgag tgagatgaac cgagactctc aaactccacg
     2761 ttcgagcaat ctcctatctc cggaagagga agatcgcatc cgtgagcagg ttcgcaagcg
     2821 gttgactccg gagaggaacc ggacgatgcc tcggaattcg aacgatccgg cgcaacactc
     2881 gaacaatgcc gcac
//
LOCUS       DSCC01000373            1892 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1041565, whole genome shotgun sequence.
ACCESSION   DSCC01000373 DSCC01000000
VERSION     DSCC01000373.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1041565"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..544
                     /locus_tag="ENN56_04685"
     CDS             <1..544
                     /locus_tag="ENN56_04685"
                     /inference="COORDINATES: protein motif:HMM:PF02633.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74820.1"
                     /translation="FGGVVHPPVYVHEGFAQEYMRPVISELFNRLKQTGFRVIIGVSG
                     HNVHRMIDMINAALEPVIADGECAGVGLWEIPEDDSGESGTDHAAKWETSNMMFFYPD
                     LVDMAALGTEPLMPRMQPPDGVGGLDPREHASAEVGRRNVEICSEALGAKARELLESL
                     PPNKRDFAIDAIVPGSWWMV"
     gene            529..>1892
                     /locus_tag="ENN56_04690"
     CDS             529..>1892
                     /locus_tag="ENN56_04690"
                     /inference="COORDINATES: protein motif:HMM:PF07944.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74821.1"
                     /translation="MVDGMKTQTVSTQILNDPVFRPLPLGKVRPDGWLKRQLRIQADG
                     LSGHIDEFWPDIAESKWFGGKAEGWERAPYWLDGFIPLAYLLDDAALIERANSRMDYI
                     IDHQREDGWLGPDDVERDVWPLFLIVKSLLQYAEASGSDRPIQAADRLLRRIDRWIGT
                     HGLCAWAQMRWPELALVAQAMCDRTGDRWFAELSARAARFGYDWVTHFIEFPFREKST
                     RWDMDTHVVNHAMALKLAAILYRLSGAEDDRDLARVAFEQLDCWHGQANGMFTGDEAL
                     AGLMPSQGTELCAVVEAMYSLEILVGTLGEAWLGDRLEKIAFNALPATFSPDMWAHQY
                     DQQANQIRCAVIEDCVYTTNGSDSNIFGLEPNYGCCTANMHQGWPKFAAHLWMSTRDA
                     DGTEGLAAIAYAPCTVHTTVNDTDVTLSVATRYPFEDSVIITVHTETPVRLPLLLRIP
                     TWCES"
BASE COUNT          385 a          547 c          584 g          376 t
ORIGIN      
        1 cttcggcggc gtggtgcatc cgccggtata tgttcacgaa ggcttcgcgc aggagtacat
       61 gcgaccggtg atctcggagc ttttcaatcg gctcaagcaa accggattcc gcgttatcat
      121 tggtgtatca ggtcataacg tgcaccgtat gatcgacatg atcaatgccg cgctcgaacc
      181 ggtgatcgct gatggcgagt gcgcaggcgt cggtctctgg gagattcccg aagacgacag
      241 cggcgaatcg gggactgatc acgcggcgaa gtgggaaaca tcgaacatga tgttcttcta
      301 tcccgacctc gtcgatatgg cggcactcgg aacggagccg ctgatgccgc gtatgcaacc
      361 acccgacggc gtcggcggtc tcgacccgcg agagcacgca tcggcggagg ttgggcgccg
      421 caacgttgag atctgctccg aggcgttggg tgcgaaggca cgcgagcttc tggaatcgct
      481 cccacccaac aagcgggatt tcgcaattga cgctattgtt cccggttcat ggtggatggt
      541 atgaaaacac aaacggtctc gacacagatt ctgaacgatc cagtattccg accgctccca
      601 ctcggaaaag tgcgacccga cggttggctg aagcggcagc ttcgtatcca agccgacggc
      661 ctttcgggac atatcgatga gttttggccc gacattgccg agagtaagtg gttcggaggg
      721 aaagcggaag gatgggaacg cgcgccgtat tggctcgacg ggttcattcc gctggcgtat
      781 ctcctcgatg acgctgcact gatcgagcgc gcgaactcca gaatggacta catcatcgac
      841 catcagcgcg aggacggctg gctcggtccg gacgacgtcg agcgcgatgt gtggccgctg
      901 ttcctcatcg tgaaaagcct gctccaatac gccgaagcga gcggcagcga tcgtcccatt
      961 caagctgccg accgactctt gcgccgtatc gaccgttgga tcgggacgca tggcctgtgc
     1021 gcatgggcgc agatgcggtg gcccgaactt gcactcgtcg cgcaggcgat gtgtgatcgg
     1081 accggcgacc ggtggtttgc ggagctatcg gctcgtgcag cgcggtttgg atatgactgg
     1141 gtgacgcact tcatcgagtt cccgttccgc gagaaatcca cgcgatggga tatggatacc
     1201 cacgtcgtca accacgcgat ggcgctcaag ctcgcggcga ttctgtaccg actatccggt
     1261 gcggaggatg atcgcgacct cgcacgggtg gcgttcgagc agctcgactg ctggcacggt
     1321 caagcaaacg gaatgttcac cggcgacgaa gcgctcgccg ggctcatgcc gtcgcagggg
     1381 accgagctct gcgccgttgt cgaggcgatg tactcacttg agatactcgt cgggacgttg
     1441 ggcgaggcgt ggcttggcga ccgcctcgag aagatcgcgt tcaacgcgct tccggcgacg
     1501 ttctcgcccg atatgtgggc gcatcagtat gatcagcagg cgaaccaaat tcgctgcgcg
     1561 gtcattgaag attgcgtcta cacgacgaac ggttccgact caaacatctt cggactcgaa
     1621 ccaaattacg gctgctgcac agcgaacatg caccaagggt ggccaaaatt tgccgcgcat
     1681 ctttggatgt cgactcgcga tgccgacggc acggaaggac ttgccgcaat cgcatatgca
     1741 ccctgtaccg tgcatacaac ggtaaacgac accgacgtga cactgagtgt cgcaacccgg
     1801 taccccttcg aggactccgt tatcattacc gttcatacgg agacaccggt tcgtctgccc
     1861 ctgctcttgc gcataccgac atggtgcgag tc
//
LOCUS       DSCC01000374            2528 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1028057, whole genome shotgun sequence.
ACCESSION   DSCC01000374 DSCC01000000
VERSION     DSCC01000374.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1028057"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..745)
                     /locus_tag="ENN56_04695"
     CDS             complement(<1..745)
                     /locus_tag="ENN56_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74822.1"
                     /translation="MSIRVTTDIPFGNAANVLVSDADSVPEISFTSHPHGGTESLWFC
                     FRAIADGSPAPRLRIVLTNPDTLLGVGHGDHVHPVVRRNADSPDSDWERLPGGTVQEL
                     PDGRWNVIWDVERPGTSCDFAVCYPYGPDELATLLTDTNRAFSIDTIGVSQHGRPIPR
                     LSNDAGSPGGKRAGIFLMARQHSGETTGSWVLDGMLREFARHGDRAPLVWAIPFSNLD
                     GVIEGDYGKDPFPWDLNRAWSSPAMRHEIL"
     gene            complement(895..1938)
                     /gene="queA"
                     /locus_tag="ENN56_04700"
     CDS             complement(895..1938)
                     /gene="queA"
                     /locus_tag="ENN56_04700"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDS74823.1"
                     /translation="MDQDWLRSSYAYELDQSRIAQSPVDPRDESRLFVYDRGTERKTH
                     VRFRDIEQYLRKGDLLVVNTTRVLPARLKPRRQDTGSEAEVLLLHQRNASEWEALVKP
                     GKRLHPGIHLTFDDETTVEIAERLPEGRRLVRFSRPIDLCWLESVGSMPLPPYIHRQA
                     TSLDTESYQTVYANEPGAVAAPTAGLHFTPELLARLDESGVHRATVLLHVGIGTFRPV
                     RVDDVRTHPMDAEYYEVSAETLRQIRETKRRGGRVIAVGTTSVRTLETLARTGELTSD
                     RDVSGWTRLFLYPPHQMQVVDAIITNFHLPESTLLMLVAAFTGRERTLELYREAIDLG
                     YRFYSYGDAMLIL"
     gene            complement(1941..2156)
                     /locus_tag="ENN56_04705"
     CDS             complement(1941..2156)
                     /locus_tag="ENN56_04705"
                     /inference="COORDINATES: protein motif:HMM:PF11146.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HDS74824.1"
                     /translation="MTLAKWFLRTGMVLVALGVALTAVLRIRYKPVAPGDQTFSIGKR
                     TIQLPLGTGIIVLLLSTLYLNTLGRQR"
     gene            complement(2153..2398)
                     /locus_tag="ENN56_04710"
     CDS             complement(2153..2398)
                     /locus_tag="ENN56_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74825.1"
                     /translation="MKRTHEPCPAGIEDRCPIPRYRCREFGEPGKHCFELFAEFEAFV
                     LPSDEPDTDDDTSRSEIDMAVLQQLRAQGISDEEIES"
BASE COUNT          549 a          677 c          727 g          575 t
ORIGIN      
        1 ccaatatctc gtgtcgcatt gcgggtgagc tccacgcacg gttcaaatcc cacgggaacg
       61 ggtctttccc gtaatcgcct tcaatcacgc cgtcgagatt gctgaacggg attgcccata
      121 ccaacggtgc gcggtcaccg tgacgagcga actcacggag cataccgtcg aggacccacg
      181 aaccagtcgt ttccccactg tgttgccgag ccatgagaaa gattccggca cgcttcccgc
      241 ccggagagcc agcatcgttg ctgaggcgtg gtatcggtcg tccgtgttgg gagactccga
      301 tggtgtctat tgagaacgct ctattcgtgt cagtcagaag ggttgcgagt tcatccggac
      361 cgtacggata gcacacggcg aaatcgcacg atgtccccgg tctttcaacg tcccagatga
      421 cgttccaccg tccgtctggc aactcttgga cggtgccccc cggtaagcgc tcccagtcgg
      481 aatccggaga gtcggcgttc cgacgaacaa ccggatgcac gtgatctccg tggccgacac
      541 caagaagcgt gtccggattc gtaagtacaa ttcgcaagcg cggtgccggt gatccgtccg
      601 cgatagcgcg gaaacagaac cacagcgact ctgttcctcc atgcggatgc gacgtaaatg
      661 agatctccgg tacgctatcg gcatcgctca cgagtacgtt tgcggcgttg ccgaagggaa
      721 tgtctgtggt gacgcgaatg gacataggtg agtcctcctc tccagagaag aacccgagaa
      781 cagtatccgt gcgcaaggct cttacctgcg cgattgacga atcgagtctg gaaaaactgt
      841 tcttaacgac gacagacgga gcagtgtacg accgtgagca gctcgtggag aaaatcagag
      901 aatcaacatc gcgtcgccgt agctgtagaa tctatagccg agatcaatcg cctctcggta
      961 gagctcgagc gttcgttcgc gaccggtgaa cgcagctaca agcatgagta gcgtcgattc
     1021 cggtagatgg aaattcgtga tgatggcgtc tacgacctgc atctggtgcg gcggatagag
     1081 aaacaaccgg gtccaacctg atacatcgcg atcggaagta agctcgccgg ttcttgccag
     1141 tgtctcgagt gtgcgtaccg acgtcgttcc gacggcgatg acgcgacctc cgcgacgttt
     1201 tgtttcgcga atctggcgaa gcgtctcagc actcacctcg tagtattcgg cgtccattgg
     1261 gtgtgttcgt acgtcgtcga ctcgtacagg acgaaacgtt cctataccga cgtggagcaa
     1321 taccgtcgct cgatgaactc cggattcatc gagacgcgcg agtagttcgg gtgtaaaatg
     1381 caatcccgca gtaggcgctg ctacggcacc tggctcgttc gcatagacag tttggtagga
     1441 ttcggtatcc aaagatgtag cttgtcgatg gatatacggg ggaagtggca ttgaaccgac
     1501 ggattccagc cagcataggt cgatcggtcg cgagaagcgc acgagtcggc gtccttccgg
     1561 caggcgttca gcgatttcga ccgtcgtttc gtcgtcgaag gtaagatgaa ttccggggtg
     1621 taatcgcttt cccggtttga cgagtgcttc ccattctgac gcgtttcgtt gatgaagaag
     1681 cagaacctca gcctcagaac cggtatcttg tcttcgcggc ttaagtcgag cgggtaggac
     1741 acgcgtcgta tttacgacga ggagatcacc cttcctaagg tattgttcga tatcgcgaaa
     1801 ccggacgtgt gttttccgct ccgttccacg gtcgtagacg aagagacgtg attcgtcgcg
     1861 aggatcgaca ggtgattgtg cgattcgact ttggtcaagt tcgtacgcgt atgagcttcg
     1921 tagccaatct tgatctatcg tcaccgttgg cggccgagag tgttgaggta cagtgtggaa
     1981 agcaggagga cgataatacc agtgccgaga ggtagctgta tcgtacgttt tccgatcgag
     2041 aacgtttgat ctccaggcgc cacaggctta taccgaatgc gtaatacggc tgtaagcgct
     2101 acgccgagcg ctactaacac catacccgtt ctcagaaacc acttcgccaa cgtcatgatt
     2161 cgatctcctc gtcgctgatg ccttgtgcgc gtaattgctg taacaccgcc atgtcgattt
     2221 cgctacgact cgtatcgtca tcggtatcgg gttcgtcgct cggaagtaca aacgcttcaa
     2281 actccgcgaa aagttcgaag cagtgctttc ccggttcgcc gaattcgcga caacgatatc
     2341 gcggtatcgg gcaccgatct tctattccgg ccggacaggg ctcgtgcgtt cgtttcacgc
     2401 gttgctccaa gtggtatgcg gtggtatcgg ttgaccaacg aaggcgtgga ttcgatactc
     2461 tcatccgagg actaacgatg caagcgaagg caggagtcct gcggacagtt tctttgtcgg
     2521 atattgag
//
LOCUS       DSCC01000375            1838 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043248, whole genome shotgun sequence.
ACCESSION   DSCC01000375 DSCC01000000
VERSION     DSCC01000375.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043248"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(10..873)
                     /locus_tag="ENN56_04715"
     CDS             complement(10..873)
                     /locus_tag="ENN56_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDS74826.1"
                     /translation="MSGRIYFADNLDVLRTLPSSSVDLIYIDPPFNTGKVQRRTQLKT
                     IRSSEGDRVGFQGIRYDSTVIGTKAFPDLFDDYLAFLKPRLVEAHRVLASHGSLYFHV
                     DYREVHYCKVLLDTIFGRESFLNEIIWAYDFGGRPKNRWPAKHDNILLYVKNPSDYVF
                     NYDEIERIPYMAPGLVGPEKAARGKLPTDTSWHTIVPTNGSEKTGYPTQKPLGVLRRI
                     VQASSPPGGTVLDFFAGSGTTGIAALELSREFILVDSNPEALAVMAKRFVDVRDIEWM
                     GFDPTPYQTHS"
     gene            complement(896..>1838)
                     /locus_tag="ENN56_04720"
     CDS             complement(896..>1838)
                     /locus_tag="ENN56_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002248492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDS74827.1"
                     /translation="NDLGLAVANSIAGVAAGARQVECTINGLGERAGNAALEEIVMAL
                     YVRNDLMGIGTNINTKEITRTSRLVSRHTGMVVQANKAIVGANAFAHESGIHQDGVLK
                     HRQTYEIMTPESVGLESNRMVLGRHSGRHGFKARIEQLGYALDGDELQRAYERFLAVA
                     DKKKEVFDEDLTAILEDEVSDVPEVYRLDYLHCLSGTGVLPSATVRIMINGVPHQESA
                     WGDGPVDAAFNAINKVVGAKVELDRYAIQAVTSGTDAIGGVNLRIRANGHVIGGHGTS
                     TDIVEASAKAFIDALNRLAFLQKRTDRNAATEVHETV"
BASE COUNT          358 a          569 c          502 g          409 t
ORIGIN      
        1 atgatgttgt tacgagtgcg tctgatacgg tgtcggatcg aatcccatcc actcgatatc
       61 tcgcacatcg acaaaccgtt ttgccatcac cgcgagcgcc tctggattac tgtcgacaag
      121 gatgaattcg cgtgacaact ccagcgcagc aatgccggtc gtgccgcttc cggcgaagaa
      181 atcgagtacc gttccgcccg gcggtgatga cgcctgcacg atccgtcgca gaacgccaag
      241 cggcttttgt gtaggatacc ccgtcttttc cgagccgttt gtaggtacga tggtgtgcca
      301 cgacgtatca gtcggcagct tcccccgcgc ggctttttcc ggtccgacta atcccggtgc
      361 catgtacggg atacgttcga tctcgtcata attaaatacg taatccgacg gattcttcac
      421 gtagagcaat atattgtcgt gcttcgccgg ccaccgattc ttcggacgtc cgccaaagtc
      481 gtacgcccaa atgatctcgt tgagaaacga ctcgcgaccg aaaatcgtgt cgaggagcac
      541 tttgcagtaa tgcacctcgc gatagtcgac gtgaaagtac agactcccgt gagatgcaag
      601 cacgcgatgc gcttcgacca gacgcggctt caaaaacgcc agatagtcgt cgaagaggtc
      661 ggggaacgcc ttcgttccga tgaccgtgct gtcgtagcgt atcccctgaa aaccgacgcg
      721 atcgccttca gatgaccgga tcgtcttcaa ctgcgttcgc cgttgaacct taccggtatt
      781 gaacggcgga tcgatgtaaa tcaagtcgac agacgacgac ggtaatgtgc ggagtacgtc
      841 gaggttatcg gcgaagtaga ttcttccact cacgtcgcac tccgcacatt gtcaatcaca
      901 ccgtttcgtg tacctcggtc gcggcgttgc ggtctgtgcg cttctgcagg aatgcgagcc
      961 gattcagcgc gtcgatgaac gccttggccg acgcctccac gatgtcggtg ctcgtgccat
     1021 gaccgccgat gacgtgtccg ttcgcgcgaa tgcgaaggtt caccccgccg atggcatcgg
     1081 ttcccgacgt cactgcctgg atcgcgtacc ggtccaactc gaccttcgcg ccaacgacct
     1141 tgttgatcgc gttgaacgcc gcgtcgacag gaccatcgcc ccacgccgat tcctgatgcg
     1201 gaacgccgtt gatcatgatg cgcaccgtcg cagagggcag cacgccggtg ccgctcaagc
     1261 agtggaggta gtcaaggcga tacacttccg ggacgtcgct cacctcgtct tcgagaatcg
     1321 ccgtgaggtc ttcgtcgaac acttccttct tcttatctgc tacggcgagg aagcgctcgt
     1381 acgcgcgttg caattcgtca ccgtccagtg cgtatccgag ttgctcgatg cgcgccttga
     1441 atccgtgacg acccgagtga cgtccgagta ccatccgatt cgattcgaga cccaccgact
     1501 ccggcgtcat gatctcgtac gtctgccggt gtttcaacac gccgtcctga tggatgcccg
     1561 actcgtgcgc aaacgcgttc gcgccgacta ttgccttgtt cgcctgcacg accataccgg
     1621 tatgacgcga cacgagacgg ctcgtacggg tgatctcttt cgtattgatg ttcgtgccga
     1681 ttcccatgag atcgttccgg acgtagagtg ccatgacgat ctcctccaac gccgcgttac
     1741 cggcgcgttc gccgaggccg ttgatcgtgc attccacctg ccgcgctccg gccgccactc
     1801 cggcgatgct attcgccaca gcgagcccga gatcgtta
//
LOCUS       DSCC01000376            1509 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1056343, whole genome shotgun sequence.
ACCESSION   DSCC01000376 DSCC01000000
VERSION     DSCC01000376.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1056343"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1509)
                     /locus_tag="ENN56_04725"
     CDS             complement(<1..>1509)
                     /locus_tag="ENN56_04725"
                     /inference="COORDINATES: protein motif:HMM:PRK12740.1-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDS74828.1"
                     /translation="LWGYANDYDLPRLVVVNACSREHANYDQTVAQAKDRFGHTAVAA
                     QIPVNQGDGFNQIVDLLTMKAYTYESGGHGKGTPGDIPADLQDTVASLREELVDAIAE
                     SDEELLEKYLEGEELDEKTLITSLKKAIKNRLVVPILVTDAVHNIGIDRLLDWTVDLL
                     PSPADIETFEAEVLGKEGETVALPMSDSDPLCAYVFKTVSEEHVGDLSYVRVYSGGLA
                     HNMDAINTRQNSPERIGQSFFMIGRDRTDAGHIRTGDMGALVKLRNTHTGDTLCQPTR
                     QLLLKQVSWPTPTIQVAVEPKAKGDEDKLATGLHRMHEEDSTFSHVVDGELGQTLISG
                     QGELHIGVKVKQLKDRFGVEVVTSDPKIPYRETIRTKGEGHYRHKKQTGGRGQFGDVY
                     LRIEPKERGSGNEFEDAVVGGVIPGKFIPAVEKGVMEGLMSGIIAGAPVIDVKATVYD
                     GSYHAVDSSEIAFKISGMYAFRQAMEQAGPILLEPIYNLEVIVPEEFMGDVMG"
BASE COUNT          293 a          447 c          394 g          375 t
ORIGIN      
        1 cccattacgt cacccatgaa ctcttcagga acgatcacct cgaggttata gatcggctcg
       61 aggagtatcg gtcccgcttg ttccatcgcc tgtcggaacg cgtacatacc tgatatcttg
      121 aacgcgattt cggaactgtc gacggcgtgg taacttccgt cgtatacggt ggccttcacg
      181 tcaattaccg gtgcaccggc gataataccg gacatcagcc cttccatcac gcctttttcg
      241 acggccggga tgaactttcc gggaattacg ccaccgacga ccgcatcctc gaactcgttc
      301 ccacttccgc gctctttcgg ttcgatgcgt aggtacacat cgccaaactg tccgcgtccg
      361 ccggtctgct ttttgtgacg atagtgacct tcgcctttgg tgcgaattgt ctcgcgatac
      421 gggatcttcg gatcagacgt gacgacttcc acaccgaacc gatccttgag ctgcttgacc
      481 ttaacgccga tatggagctc accttgacca gagatgagcg tctggccaag ttccccgtcc
      541 acgacgtggc tgaacgtcga atcctcttcg tgcattcggt gcaagccggt tgcgagcttg
      601 tcttcgtcgc ctttcgcttt cggttctacc gctacttgga tggttggtgt cggccacgat
      661 acctgcttga gaagaagctg acgcgtaggt tggcagaggg tgtcaccggt atgcgtgttg
      721 cgcagtttca cgagcgcgcc catgtcgccg gtacggatgt gtcccgcgtc ggtccggtcg
      781 cggccgatca taaagaaact ctgtccaata cgttccggcg agttctgtcg cgtatttatc
      841 gcgtccatgt tgtgagcgag gccaccgcta tacacgcgca catacgacag gtcaccgacg
      901 tgctcttcgc tgactgtctt gaacacatac gcgcagagcg gatcggaatc ggacatcggc
      961 aacgcgaccg tctcaccttc cttacccagt acttccgctt cgaacgtctc aatatcggcg
     1021 ggtgacggga gaagatcgac tgtccaatcg agcaaacggt cgattccgat attgtggacc
     1081 gcgtccgtca cgaggatagg cacgactaat cggtttttga ttgctttctt caaactggtg
     1141 atcagtgtct tctcatctaa ctcttcgcct tcgagatact tctcgagaag ttcctcgtcg
     1201 gattccgcga ttgcgtcgac gagttcctcg cgcaacgaag cgaccgtatc ctgtaaatcg
     1261 gcgggaatat cgccgggcgt gcccttcccg tggccacccg attcgtatgt atacgctttc
     1321 atcgtgagca gatcgacgat ctgattgaaa ccgtcacctt gatttacggg aatctgcgcg
     1381 gcgacggctg tatgcccgaa tcgatccttc gcctgtgcca cggtctgatc gtaattcgcg
     1441 tgttcgcggc tacatgcgtt tacgacaacc aaccgcggga gatcataatc gttcgcatac
     1501 ccccacagg
//
LOCUS       DSCC01000377            2250 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032777, whole genome shotgun sequence.
ACCESSION   DSCC01000377 DSCC01000000
VERSION     DSCC01000377.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032777"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..758)
                     /locus_tag="ENN56_04730"
     CDS             complement(<1..758)
                     /locus_tag="ENN56_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74829.1"
                     /translation="MRGMNLPRVRQFVAIVCALGIAVYVAACGSAARRVQLSVPSMDM
                     PVSMTVRIPHDKNVFDLPLIVILDDGPPVDVTLTHPQRHTIDQFESILLREGYPVWRP
                     FRELWQKGDLTVHCPDRLVSWTLLGLRAARDITQIDESKMIIIGFGQGGIVGLVSASR
                     SAELTHAVAIVGTPARSIDVVLTSPALRDSVTRRRLLDTFGRIWANEYPDTAIVMHGE
                     AQCWRAWLQITKGILELVDAVPQPILAVQGTADTL"
     gene            992..2248
                     /locus_tag="ENN56_04735"
     CDS             992..2248
                     /locus_tag="ENN56_04735"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDS74830.1"
                     /translation="MTSRERVRTALSHREPDRVPIDLGGMGSTGIMTVAYNKLKSHLG
                     LNTRPSRMYDTGQQLAEPEAEILERFRVDVIALSRTYSPVTDPELSREYEAKYGGSWK
                     PWTLPDGSSCEVPDGFKPEPDGQGGWVLKNERGVYAAMPKGALYFDASGPAANELGTA
                     ETVEDLASWKLHYAEPEQFSSLREKAKWLHENTDFAIMCGFGGGTHEPAQGLRGWGQW
                     MMDLAADRTLADEIIGRLTEAHVHNVKLFMEAVGPYLDIVQMGDDLGTQAGPQMSPQM
                     YHETIHPAHKAVYQAARAAHEYPFIFLHSCGGIQPLIPDLIDEGVDVLNPVQTNAAGM
                     DPAGLKRDFGDKLAFWGGGVEVAGALTDGTPADVKRQVRERLRIFAPGGGYVFNQVHN
                     IQANVPPANIVAMFDAAYEYGKYPIA"
BASE COUNT          475 a          660 c          681 g          434 t
ORIGIN      
        1 agagtatcgg ccgttccctg cacggcgaga atcggttgcg gtacggcatc gacaagctcg
       61 aggataccct tggtgatctg gagccacgcc cgccagcatt gtgcctctcc gtgcatgact
      121 atcgccgtat ccggatactc gttcgcccag atacgaccga acgtgtcgag caagcgccgc
      181 cgcgtgaccg aatcccgcag cgccggtgac gtgagcacca catcaatcga ccgagccggc
      241 gtcccgacga ttgcgaccgc gtgcgtgagt tcggcgctgc ggctcgcaga caccaatcca
      301 acgatgccgc cctgcccgaa tccgatgatg atcatcttag attcgtcgat ctgtgtaatg
      361 tcgcgcgcgg cgcgaagtcc gaggagcgtc cacgacacga gacgatcggg acagtgcacc
      421 gtgaggtcgc ccttctgcca caactcgcga aacggtcgcc agaccggata tccctcgcgc
      481 aggaggatcg attcgaactg gtcgatggtg tgacgttgcg gatgcgtaag cgtcacgtcc
      541 accggcggac cgtcatcgag gattacgatg agtggcagat cgaacacgtt cttatcgtga
      601 gggatccgca ctgtcatcga caccggcatg tccatcgacg gaacggaaag ctgtacgcga
      661 cgagccgccg atccgcatgc ggcgacgtat acggcgattc caagcgcaca cacaatggca
      721 acgaactgcc gtactcgagg tagattcatg ccgcgcatag gtttacgctc tcgttcgcga
      781 cgagtctctg ccgatagtcc tatccgatac ctcacatacc acgtgatctt ccacggtgga
      841 atatgatgcg taacgatagt gatcggcaag gcgaacacgg ttttttcgcc acgcgattcc
      901 ctttccgccg atttgcaacg ttccgtattt tttctaccaa gcacgatatc attcgtgtga
      961 tatcgtacac gccaacgcaa ggagcattca tatgacgtcc cgtgaacgcg ttcgcacggc
     1021 actatcccat cgcgaacctg atcgtgtccc aattgactta ggcggcatgg gttcgaccgg
     1081 tatcatgacc gtcgcataca acaagctcaa atcgcacctc ggcctgaaca ctcgtccatc
     1141 gcgcatgtac gacacgggcc agcaactcgc cgagccggaa gcggagattc tcgagcgctt
     1201 ccgtgtggat gtcatcgcac tgagccggac atactcgccg gtcaccgatc cggagctctc
     1261 gcgtgagtat gaggcgaagt acggcggctc atggaagccg tggacactcc cggacgggtc
     1321 atcctgtgaa gttcccgacg ggttcaagcc ggaaccagac gggcagggcg gatgggtgct
     1381 caagaacgag cgtggtgtgt atgcggcgat gccaaaaggc gcgctctact tcgacgcttc
     1441 gggacctgcg gcaaatgaac tcggtaccgc ggaaaccgtg gaggatctcg cttcgtggaa
     1501 gctccattac gccgagcctg agcagttttc gtcacttcgc gaaaaggcga aatggctcca
     1561 cgaaaacacc gatttcgcga tcatgtgcgg gttcggcggt ggaacccatg agccggcgca
     1621 gggactccgc gggtggggtc agtggatgat ggatctcgcc gccgaccgga cgcttgccga
     1681 cgagattatc ggacggctca ccgaggcgca tgtgcacaac gtcaagctgt tcatggaagc
     1741 ggtcggtccg tatctcgaca tcgtccagat gggcgacgat ttgggtacac aggccggtcc
     1801 acaaatgtcg ccgcagatgt accacgagac gattcatccg gcgcataagg cggtatatca
     1861 agcggcgcgc gccgcgcacg agtatccgtt catcttcctc cactcgtgcg ggggaattca
     1921 gccactgata cccgatctca tcgatgaagg cgtcgatgtg ctgaatccgg tgcagacgaa
     1981 tgcggcggga atggatccgg cggggttgaa gcgggatttc ggtgacaagc ttgcgttctg
     2041 gggcggtggc gtggaagtcg ccggcgcgct taccgatggc acaccggcgg acgtgaagcg
     2101 gcaggttcgc gagcgccttc ggattttcgc accgggaggc ggctacgtgt tcaatcaggt
     2161 gcacaacatt caagcgaatg tgccgcccgc gaacatcgtt gcaatgttcg acgcggcgta
     2221 tgagtacggg aagtacccga tagcctaacg
//
LOCUS       DSCC01000378            2250 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1032772, whole genome shotgun sequence.
ACCESSION   DSCC01000378 DSCC01000000
VERSION     DSCC01000378.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1032772"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..584)
                     /locus_tag="ENN56_04740"
     CDS             complement(<1..584)
                     /locus_tag="ENN56_04740"
                     /inference="COORDINATES: protein motif:HMM:PF00501.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74831.1"
                     /translation="MGRQRMTGYAAREHHGILTTPEAVAYGAAQWGDDVALRVWRDGT
                     YQEITRTELSNRVDALAAGLMSLGVRPKNLVALLGENRPEWAIGYLGIHRAGASVVPL
                     DSLLTPRELRPLLIDSAVSVIIVSPRFIDDILELKGSIDTLETVICMDDDVAASKDST
                     HVLGMEAVSRRADGETPVFPEVTLDDLAALIYTSG"
     gene            complement(701..>2250)
                     /locus_tag="ENN56_04745"
     CDS             complement(701..>2250)
                     /locus_tag="ENN56_04745"
                     /inference="COORDINATES: protein motif:HMM:PF13193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDS74832.1"
                     /translation="VLGENRPEWAIAYMAIHRAGAVVVPLDSLLKAGEFRHIIGDAGV
                     KAVIVSSKFVADILEVRDAIGEPTTIICMDDEHANSDENILAMSAVMSDELPETWPEV
                     TLDSLAAIIYTSGTTGQSKGVMLSQMNLGSDAAGSYQTIFFGDNDNFLSVLPLHHTFE
                     CTGGFLLAVYGSSTITYARSLKSRDIIEDVKNTNATIMLGVPLLFEKMMEGIQRKLAQ
                     QPATKRALIATLFGIEKLGRKVGLKLGGKLFHSLREKAGLASLRLLISGGAALPPHVA
                     EWFNSLGFLLFQGYGLTETSPVTNINRLDCIVHSSVGQPLPNCEVKIIDVVDGHGEIC
                     VRGPMIMTGYYKNEEATREVIDEEGWFHTGDIGYTDEIDRLFISGRKKNVIVTPSGKN
                     VYPEEVEHHINQSPYVLESLVIGRPIAGTTSEDVYAMVVPHYEYFDEQGTERGKEFTT
                     EEIEKIVRDEVRAAIDNIAEYKRPKQFEIRSEEFEKTSTKKIKRFLYKQKPISVDENG
                     TNGKRSH"
BASE COUNT          485 a          631 c          588 g          546 t
ORIGIN      
        1 cccgacgtgt aaatcaacgc agcgaggtcg tcgagtgtga cttctggaaa gacgggcgtt
       61 tcgccatctg cgcgtcgcga aactgcttcc ataccaagta catgcgttga gtctttggag
      121 gccgcgacgt cgtcatccat gcagatgacc gtttcgagcg tgtcgatact ccccttcagc
      181 tcaaggatgt cgtcgataaa ccgcggcgag acgatgatca cgctcactgc actatcgatc
      241 agaagcggac gcagctctcg tggtgtgaga aggctatcga gcggaacaac cgatgcaccg
      301 gcgcgatgga taccaagata cccaatcgcc cattccggac ggttctcacc aagcaacgcg
      361 acaagatttt tcggacgaac gcctaacgac atgagtcccg ccgcgagagc gtccacacga
      421 tttgacagtt cggtccgagt aatttcctga tacgttccgt cgcgccatac acgcaatgca
      481 acgtcatcac cccattgcgc agcgccgtag gctaccgctt cgggtgtggt caagatgcca
      541 tgatgttcac gggctgcgta accggtcata cgctgcctcc ccatgtgatt gtgagttaag
      601 tcgaagaaaa cacgtaagta aaacccgatg tgtgtgtacg cacaacgacg caaccgagga
      661 tgtatcggtt gcgtcgttgg ggcgtacagt aggcttcggt ttagtgagag cgcttcccat
      721 ttgttccatt ctcgtcgaca ctgatcggtt tctgcttgta aaggaagcgc ttgatcttct
      781 ttgtcgaagt cttttcgaac tcttccgagc gaatctcgaa ctgcttcggt cgcttgtact
      841 cggcgatgtt gtcgatggcg gcgcgaactt cgtctcggac gatcttctct atttcctctg
      901 tcgtaaattc cttaccgcgc tcagtgccct gttcgtcgaa gtattcgtaa tgcggtacaa
      961 ccatcgcata tacgtcttcg ctggtggtac cggcaatcgg acgcccgatt acgagtgatt
     1021 caagaacgta cggactctgg ttgatgtggt gctcgacttc ttccgggtaa acattctttc
     1081 cactcggcgt tacgatgacg ttcttcttcc gtccgctgat aaagaggcga tcgatctcgt
     1141 ccgtgtaccc gatatcgccc gtgtggaacc agccctcttc atcgatgact tcccgcgtcg
     1201 cctcttcgtt cttgtaatag ccggtcatga tcatcggacc gcgtacacat atctcgccgt
     1261 gtccgtcgac cacatcgatg attttcactt cgcagttcgg tagcggctgc ccaacagagg
     1321 agtgaacgat acaatcgagt cgattaatgt tggtaaccgg cgaggtctcg gtgagtccgt
     1381 acccttggaa aagaaggaag ccaaggctgt tgaaccactc agcgacgtgt ggcggaagtg
     1441 ctgccccacc ggagatcagc aaccgcaaac ttgcaagacc cgctttttcg cgcaagctgt
     1501 ggaaaagctt cccgccgagc ttcagcccaa cctttcgccc gagcttttcg atgccgaaca
     1561 acgttgcgat aagtgctcgc ttcgttgccg gctgttgcgc gagctttcgc tggatgcctt
     1621 ccatcatttt ttcgaagaga agcggaacgc caagcataat cgttgcgttg gtattcttca
     1681 cgtcttcaat gatgtcgcgc gacttgagac ttcgtgcgta ggtaatcgtg gaactgccat
     1741 agaccgcaag gaggaatcct ccggtacatt cgaaggtatg atgcagaggt agcaccgaaa
     1801 ggaaattgtc gttatcgccg aagaagatgg tctgataact acccgcggca tccgagccaa
     1861 ggttcatttg actcagcatt acaccctttg attgtccagt cgttccgctt gtgtagatga
     1921 tagccgcaag gctatccaac gtcacttccg gccatgtctc aggcaactcg tcggacatga
     1981 cggcactcat ggcgagaata ttctcgtctg aattggcgtg ctcatcatcc atgcagatga
     2041 tcgtcgttgg ttcgccaatc gcatcccgca cttcgaggat atccgcgacg aatttcgacg
     2101 atacgatgac cgccttcaca ccggcgtcgc caatgatgtg ccggaactct ccagccttga
     2161 ggagcgaatc gagcggaacg actacggctc ccgcgcgatg tatcgccatg tacgcgattg
     2221 cccactccgg ccgattttcg ccgagtacgc
//
LOCUS       DSCC01000379            1540 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1054915, whole genome shotgun sequence.
ACCESSION   DSCC01000379 DSCC01000000
VERSION     DSCC01000379.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1054915"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..224)
                     /locus_tag="ENN56_04750"
     CDS             complement(<1..224)
                     /locus_tag="ENN56_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS74833.1"
                     /translation="MTDAESLHARVQERNGSTPQLSLVIPLLNEVESIRPLYDRIISV
                     LEAESYTAEIIFVDDGSRDGSYDVIGQIAE"
     gene            complement(221..1189)
                     /locus_tag="ENN56_04755"
     CDS             complement(221..1189)
                     /locus_tag="ENN56_04755"
                     /inference="COORDINATES: protein motif:HMM:PF03706.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDS74834.1"
                     /translation="MLRSVIFSRPAKLFISIALIAAVIWQVGPHAVVPETIRWSWIVV
                     AIALFLLSNIFGAFQWNLLLRCAGLRLPVFTVIRAYFIALFFNNFLIGNVGGDVLRAI
                     DVRTRVERGQGKTEAAIATIVMDRFLGFFTMMCFAVGAALFATERLESGAILFILLAA
                     FVVVGTLITSRRVGLFADRWIIRILPARLAVTIVRLRAGFVAMRTSPVVLAQAFAISA
                     FVQGIRIIVHYVCSLAIGLDIPFLYFVTFIPLVSVAAAIPISFGGLGTREWAAVGLFK
                     TVGVGGAGIVAMELIAHGVTLVASLPGAIDFVTRKNTSRDVSKESI"
     gene            complement(1191..1520)
                     /locus_tag="ENN56_04760"
     CDS             complement(1191..1520)
                     /locus_tag="ENN56_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74835.1"
                     /translation="MMVLKNTGGFALFFVVPVRIGLELITFLGALLTGRLRRAAAAVA
                     GLFGTVGHARHIRRERRRIAMFRRRSDRDVARMMYGGSAALRYLFGRPAPCAPTGLES
                     RKSGGVT"
BASE COUNT          397 a          427 c          417 g          299 t
ORIGIN      
        1 ctctcggcga tttggccaat aacgtcatat gaaccatcgc ggcttccatc gtcgacgaaa
       61 atgatctcag cagtatagga ttcggcttcg agaacggaga tgattcgatc gtagagcggg
      121 cgtatacttt cgacttcgtt gagtaacgga atcacgaggc tgagttgcgg agtcgatccg
      181 ttacgctcct gcactctcgc gtgcagagat tcggcatcgg tcatatcgac tcctttgaaa
      241 cgtcgcgaga tgtgttcttc ctcgttacaa aatctatcgc accgggtagg ctcgcgacca
      301 acgtcactcc gtgtgcaatt aattccatcg cgacgattcc ggcacctccg acgccaacag
      361 ttttgaataa gcctaccgcg gcccattcac gtgtcccaag tccaccgaaa cttatcggaa
      421 tcgccgcagc cacgctcacg agcggaatga atgtgacgaa gtagaggaac ggtatatcaa
      481 gtccgatcgc gagcgaacac acgtaatgta caatgattcg tatgccctga acgaatgcgg
      541 agatcgcgaa tgcttgggca agcacaaccg gactcgtgcg catggcaacg aacccggcac
      601 gtagtcgcac gatcgtgacc gcgagtctgg ccggcagaat gcgaattatc cagcggtcgg
      661 cgaacagacc gacacgacgg ctcgtgatca aggtgccaac tacgacaaaa gcggcgagaa
      721 gaatgaataa aatcgcaccg gattcaagac gttcggttgc gaacagcgcg gcaccgacgg
      781 cgaagcacat catcgtgaag aaaccgagga agcggtccat gacaattgtc gcaatggcgg
      841 cttcggtttt cccttgtcct cgctcgacgc gggttcggac gtctattgca cggagcacgt
      901 caccgccgac attcccaatg aggaagttgt tgaaaaacaa cgcgatgaaa tacgcgcgga
      961 taacggtgaa tactggaagc cgaagcccgg cgcatcggag gagaagattc cactggaacg
     1021 ccccgaatat gttgctgagg agaaataggg ctatcgcaac gacgatccag ctccatcgaa
     1081 tcgtttccgg aactaccgcg tgtggcccaa cctgccatat gacagccgcg atcagcgcaa
     1141 tactgatgaa tagtttggcg ggacgggaaa agatcaccga acggagcatg ctatgtcacg
     1201 cctccgcttt tccgtgactc taatcccgta ggtgcacacg gtgcgggacg tccaaataga
     1261 taccgaagcg cagcactgcc accgtacatc attcgtgcga catctctatc tgaccgtcga
     1321 cggaacatcg cgattcggcg gcgttctctt cgaatatgcc gcgcgtgtcc cactgtgccg
     1381 aacagaccgg caactgcagc ggcggcacga cgcaagcgac ccgttaagag tgcaccaaga
     1441 aacgtgatga gttcgagccc gattcgaacc gggacaacga agaacaacgc gaaaccgcca
     1501 gtgtttttga gcaccatcat gaagctattt cggtgattca
//
LOCUS       DSCC01000380            1575 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1053234, whole genome shotgun sequence.
ACCESSION   DSCC01000380 DSCC01000000
VERSION     DSCC01000380.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1053234"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(94..1353)
                     /locus_tag="ENN56_04765"
     CDS             complement(94..1353)
                     /locus_tag="ENN56_04765"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS74836.1"
                     /translation="MASVSLHTLRDLRTWRIRIFVVTWLAYVGYYFTRKHLPVVQPLF
                     LEEYGWSMFDLGVIAAAFQLTYAVGQLVMGAVGDRFGARRLLLVGMIATLAMSLFSGL
                     ATTVSAFIVIWLVNGFAQATGWPATVATMARWFPRKTRGRVMGWWTTNYMVGDTLATW
                     LAAASIAAAPTALTAIRWGFWIPALAFCVVIFVVWKWHREAPEDVGIYEVPDTNGPNG
                     RAVHWRDLAAVVKVPGLWVAAVITGALKFAMYVFFFWTVTYLVDARGYSDAAAGYLAA
                     LLPLGGAAGAICAGWTSDRLFTGRRIPVAVILLFVLAVVTVAIPYTSSDPYVLAALFL
                     VAGFAIYGPESLLAGAGAIEYAPAGAMGTAVGFVNAIGSLAGIPAGLATAAIAESFGW
                     EAVFWMVAALAVITMFGALPLWNSRGK"
     gene            complement(1428..>1575)
                     /locus_tag="ENN56_04770"
     CDS             complement(1428..>1575)
                     /locus_tag="ENN56_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDS74837.1"
                     /translation="AEFEGEKGVIFHNVLVRVAKGLVPELHLDTDDANAADLENGDML
                     RIIV"
BASE COUNT          365 a          478 c          454 g          278 t
ORIGIN      
        1 cgaatatcga tgtcgtcagg gacggtaacc tccaggtcgt tgcgcccata tttcatctgc
       61 acattcatgc gtgactccgt tccactcgca tcgttatttt ccgcgtgagt tccacagcgg
      121 caacgccccg aacatcgtta tgaccgccag cgcggcaacc atccagaaca ccgcttccca
      181 tccgaacgac tcggcgatcg cagcggttgc gagtcctgcc ggaatacctg ccagcgaacc
      241 gatggcgttg acgaagccga ccgcggtgcc cattgcaccg gctggcgcat attcaattgc
      301 accggcaccc gcgaggagtg attccggacc gtatatcgca aatcccgcca cgagaaacaa
      361 cgccgcgagc acgtacggat cggacgacgt ataggggatc gccaccgtaa cgacggcgag
      421 tacgaagaga agtattacgg caaccggaat acgccgcccg gtaaatagcc ggtcggaagt
      481 ccatcccgcg caaattgcgc ccgctgcacc accaagcggg agcagtgccg cgaggtagcc
      541 cgcggcggca tccgagtatc cgcgcgcatc tacgagatac gtgacggtcc aaaaaaagaa
      601 cacgtacatc gcgaatttca atgcgccggt aatgaccgcg gcgacccaca gaccgggaac
      661 tttcacgaca gcagcgaggt ctcgccaatg cacggcgcgt ccgttcggac cgttcgtgtc
      721 tgggacctcg tagatgccca catcctcggg tgcttcgcgg tgccacttcc agacgacgaa
      781 tatcacgacg cagaatgcga gtgcgggaat ccagaatccc catcggattg ccgtgagcgc
      841 ggtcggagca gcggcgatgg acgctgcggc aagccatgta gcaagcgtat cgccgaccat
      901 gtagttcgtg gtccaccatc ccattacgcg gccacgggtt tttctcggaa accaccgcgc
      961 catggtcgca accgtcgcag gccatccggt cgcttgggcg aacccgttca ccaaccagat
     1021 gacaatgaac gcactgacgg tggttgcgag tccactgaat aatgacatcg ccagtgtcgc
     1081 gatcatgccg accagcagga gtcgacgcgc gccgaaacgg tcgccgaccg cacccattac
     1141 gagctgcccg actgcgtacg taagctgaaa cgccgctgcg attacgccga gatcgaacat
     1201 cgaccagccg tactcttcca aaaagagtgg ttgcacgacc ggaagatgtt tccgcgtgaa
     1261 gtagtagccg acatacgcca gccacgtgac gacgaatatc cggatgcgcc aggtacggag
     1321 gtcgcgaaga gtatggagag acacggaagc catgtatcgg ttaggagtgt ggaacgaagt
     1381 gtaaacaaat taagggtacg cgcgagcgga cgttttcgga aggatgttta gacgatgatt
     1441 cggagcatat ccccgttttc gagatcggcg gcgttcgcgt cgtcggtatc gagatgcagc
     1501 tccggtacca agccttttgc gacgcggacg agcacgttgt ggaagatcac acctttctcg
     1561 ccttcaaact ccgcg
//
LOCUS       DSCC01000381            4749 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1012024, whole genome shotgun sequence.
ACCESSION   DSCC01000381 DSCC01000000
VERSION     DSCC01000381.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1012024"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="ENN56_04775"
     CDS             complement(<1..2023)
                     /locus_tag="ENN56_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74838.1"
                     /translation="MPRGKTQPNHVVRRYGRVYQLRIETENDLDNLDAFGIDHWVATA
                     APITSFNCDQLFLKFLDYDGNGRISTTDIFQAKGWLFKVLKNRKNLVEGIDRTCIRDI
                     DTTSPEGQNVRIALERLITDTDISEETPVSLAQVRERKNVLLKSPVNGDGLFPPPQIA
                     DPELAAYADAVLNTVGGTDDINGLKAVGDSHVGQFHTDAAAYLAWLEEGRVPPEGETS
                     ALYPLGAATPAAFGALTAIRPKMEEYFAQCRLVRIEPNSLTAMRLQEVQLKETESTDV
                     PRITELSERAPLQMPVADEQLRFDAPVNAVYADRVARFRELVVIPLLENSGDTLTREQ
                     WLNANAMLAPYEKWLGAKRGASVEKLGEEKIRELISGDLRDRFNALLAQDKMIASEIV
                     HVENLERLLLFQSLFVRFLNNFVSLNDVYNPRRETLVRAGTLVIDGRRFSFVVRVKSR
                     AAHKAIAQQSGIYLMYLEIIPKNAGEKFEVVAPVTRGSANTLFVGKHGVFFDENGREL
                     DAVITEIVTNAISIKQAIIAPFQRAFEAMEKKVEGISAAREKEMTQQLGEVTEKAEAG
                     VQSGVQQVSTGQAQLGSAQAAQQASANSKRDWVIGGSIAFAAVGSATAFMAKTFSSLS
                     WLNILIIVLAAALIVIIPTVLIAWFKLRRRNLGSVFEGAEWAVNSRLRIT"
     gene            complement(2120..3136)
                     /locus_tag="ENN56_04780"
     CDS             complement(2120..3136)
                     /locus_tag="ENN56_04780"
                     /inference="COORDINATES: protein motif:HMM:PF12715.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDS74839.1"
                     /translation="MAERIFLMRDWVDRHLADGPTLSCNAKNRAEFSSWQTEFRRAIR
                     RALGPFPDSTPLNAEVLETTDCGSYVREKVVFDSEPNMSVPAWVLVPKNLREMAPALL
                     CAHGHGTGKNPLVGLDAEGQPFHEDYQHQLAVKFAERGYVCIAPDWRGFGERREDESY
                     TRGSRDGCNLRQLGAGYFGYNLLALQIHDGRRCIDYLISRPEVNRRRIGCIGVSFGGT
                     MTTYMAAFDRRVKAAVISCYLSTIANGLRRTNYCGAQYMPMLGELGDIADVALLSAPK
                     PILAEIGTQDTCFEIDDAMQAAEYVKRGYTAAGVADRFDTDVFEGVHEFSGRKAFDWF
                     DRWL"
     gene            complement(3192..4184)
                     /locus_tag="ENN56_04785"
     CDS             complement(3192..4184)
                     /locus_tag="ENN56_04785"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDS74840.1"
                     /translation="MHQQPINVTVTGGAGQIAYSLLFRIASGEVFGENRPVRLRLLEI
                     PPAMKALDGVVMELIDCAYPTLDGVDTFDDANEAFDGANWALLVGAKPRGKGMERGDL
                     LKDNGKIFVGQGKALQRAAKDIRVLVVGNPCNTNCLIAMHNAPDVPRDRFAAMTMLDQ
                     RRAMAQLAMKAGKGVDAVTNVAIWGNHSATQFPNFEHAKINGKPAETVIADRAWLEGE
                     FLSTVQKRGAAIIEARGASSAASAANAALYHVKSMLAPTPAGDWFSAAVPSDGSYGIE
                     PGLICGFPIQRKASTWSIVQGLPLSDYAKAKIDASVNELKEERDEVRDMLPERD"
BASE COUNT          960 a         1426 c         1321 g         1042 t
ORIGIN      
        1 gggtaatccg caaccgtgag ttcacagccc attcggcacc ctcgaaaacc gatccaagat
       61 tgcgccgtct gagtttgaac cacgctatga gaaccgttgg gatgatcaca attagagcgg
      121 ccgcgaggac gataatcaag atattcaacc agctcaacga ggagaacgtc ttcgccatga
      181 aagcggtagc agaaccgact gccgcaaaag cgatgcttcc accgataacc caatctcgct
      241 tcgaattcgc gctcgcttgt tgcgcggcct gcgccgagcc aagttgagcc tgtcccgtgc
      301 tgacttgctg gacaccgctc tgaacgccgg cttcagcctt ctctgtcact tctccgagct
      361 gctgcgtcat ctccttttct ctggcggcgg aaatcccttc gaccttcttc tccatcgcct
      421 cgaacgcacg ttggaacggc gcgatgattg cctgtttgat actgatcgcg tttgtgacga
      481 tctcagtaat gacggcgtcc aactcgcgtc cgttttcgtc gaagaacacg ccgtgtttgc
      541 cgacaaatag cgtgttcgcg ctgccccgag ttaccggcgc cacgacttcg aacttttcgc
      601 ccgcgttctt cgggattatc tcgaggtaca tgaggtagat accgctctgc tgcgcgatcg
      661 ctttgtgcgc ggcacgactc ttcacgcgta caacaaatga gaatcggcgt ccgtcgatca
      721 ccagcgtacc ggcacgaaca agtgtctcgc gacgcggatt gtaaacgtcg ttcaagctta
      781 cgaagttgtt gaggaatcgc acaaagagac tctggaacag caatagccgt tcaaggttct
      841 cgacatgtac gatctccgac gcgatcatct tatcctgtgc gagaagcgcg ttgaatcgat
      901 ctcggagatc gccggaaatc agctcgcgga tcttctcttc accgagtttc tcgaccgacg
      961 cgccccgttt tgcgccaagc cacttctcgt acggcgcaag catcgcatta gcgttgagcc
     1021 actgctcgcg cgtcaacgtg tcgccgctat tttcaagcaa cggaatgacg acaagctcgc
     1081 ggaaacgagc gacccggtcg gcatacacgg cattcaccgg agcgtcaaaa cgtagctgtt
     1141 cgtcggctac cggcatctga agcggtgcgc gctctgacaa ttcggtaata cgcggaacgt
     1201 cggtgctttc ggtctctttc agctgcactt cctggagacg catcgctgtc agtgaattcg
     1261 gctcgatgcg aacaaggcga cactgcgcga agtactcttc catcttcggg cgaatcgcgg
     1321 tcagtgcacc aaacgcggcg ggagtcgccg cgccaagtgg atatagcgct gatgtttcgc
     1381 cttccggggg aacacgacct tcttcgagcc acgcgagata cgccgctgca tccgtgtgaa
     1441 actgtccgac gtgcgaatct ccgaccgctt tcaagccgtt gatgtcgtcg gtgccaccga
     1501 cggtgttgag cacggcgtcg gcatacgcag caagctcggg atccgcgatc tgcggcggcg
     1561 ggaagagccc gtcgccattt accgggctct tcaagagtac gttcttccgc tcgcgaacct
     1621 gtgcgagact gacaggcgtc tcctcagaaa tgtccgtgtc ggtaatcaaa cgttccaacg
     1681 cgatgcggac gttctgccct tccggacttg tcgtgtcgat atcgcggata caggttcgat
     1741 ctataccttc cacaaggttt ttgcggttct tcagaacctt gaagagccaa cctttcgctt
     1801 ggaagatgtc ggttgtggag atacgcccgt tcccgtcgta atcgaggaac ttgaggaaga
     1861 gctgatcgca gttgaagctt gtgatcggtg cggcggttgc tacccagtgg tcgataccga
     1921 acgcatcaag attgtcgaga tcgttttcgg tttcgatacg gagttgatat acccgtccgt
     1981 agcgtctcac gacgtggttt ggttgagttt taccccgagg catatcggtt ctccccgctc
     2041 tttggcagga gagcgtcttg agcagcaaac gtcgtccgtg agtcaggcat ccgtccggac
     2101 agttgacttg cagctacgac tacaaccagc gatcgaacca gtcgaaggct tttcgaccgc
     2161 tgaactcatg aacgccttcg aacacatccg tatcgaagcg gtcggcgaca ccagccgccg
     2221 tatagccacg tttcacgtac tcggccgctt gcatcgcatc gtcgatttcg aagcacgtat
     2281 cctgcgtgcc gatttccgca agtatcggct tcggagcgga gagaagggca acatcggcaa
     2341 tatcgccgag ttcgccgagc atgggcatgt actgtgcacc gcagtagttt gtgcgacgca
     2401 aaccgttcgc gatcgtgctg aggtagcagg atatcaccgc cgctttaact cggcggtcga
     2461 acgcagccat gtacgtcgtc atggttccgc cgaacgagac gccgatacac ccaatgcggc
     2521 gtcggttaac ctcaggacgc gatatgaggt agtcgatgca acgcctacca tcgtgaatct
     2581 ggagcgcgag gaggttgtaa ccgaaatacc cggctccgag ctgtcgaaga ttgcatccgt
     2641 cacgtgaacc gcgcgtgtat gactcatcct cacgccgctc gccaaatcca cgccaatccg
     2701 gtgcaatgca tacgtatccg cgttcggcga atttgaccgc gagctgatgt tgatagtctt
     2761 cgtggaacgg ttgaccctct gcatcgagac caacgagcgg gttctttccc gtgccgtggc
     2821 cgtgtgcaca gaggagcgcc ggagccatct cgcggaggtt cttcggcacg agtacccacg
     2881 ccggtaccga catgttcggt tccgagtcaa agacgacttt ctcgcggacg tagctaccac
     2941 aatcggtggt ttcgagcact tccgcattga gcggcgtcga gtcggggaat ggaccgagcg
     3001 cgcgacgaat cgcgcgccgg aactcggtct gccatgagga aaactcagca cggtttttcg
     3061 cattacacga aagcgttgga ccatccgcaa ggtggcggtc gacccaatcg cgcatgagaa
     3121 agattctctc ggccattgtc gctccgactg atacatgtca ataatcacga acgcgcgaga
     3181 tgcgcgactc gttagtcgcg ctcgggaagc atgtccctaa cttcgtcacg ctcttctttg
     3241 agctcgttta ccgacgcatc gatcttcgct ttcgcgtaat cggaaagcgg aagaccttgg
     3301 acgatgctcc acgtgctcgc tttgcgttgt atcgggaagc cgcagatgag tcccggctcg
     3361 ataccgtagc ttccgtcgga gggaacagcg gcgctgaacc aatcacccgc cggagtcggc
     3421 gcgagcatgc tcttgacgtg atacaacgca gcattcgcgg cagatgcggc cgacgaagct
     3481 ccgcgcgctt cgataattgc tgcgccacgc ttctgaaccg tgctgaggaa ctcaccttca
     3541 agccatgcac gatcggcgat cacggtctcg gccggctttc cgttgatctt cgcgtgctcg
     3601 aagttcggga actgggtcgc gctgtgattg ccccatatcg cgacgttcgt cactgcgtcg
     3661 acgccttttc cggctttcat cgcgagttgc gccattgcgc ggcgctgatc gagcatcgtc
     3721 atggccgcga accggtcacg cggcacgtca ggcgcgttgt gcattgcgat gaggcagttc
     3781 gtgttacacg gattcccaac aacgagtacg cggatgtctt tcgcggcgcg ttgcagcgct
     3841 tttccttgcc cgacgaaaat ctttccgttg tctttcagga ggtcaccgcg ctccatccct
     3901 ttgccgcgcg gcttcgcgcc gacgagaagc gcccagttcg cgccgtcgaa cgcttcattg
     3961 gcatcatcga aggtgtcgac accgtccagt gtgggatagg cgcagtcgat caattccatg
     4021 acaacaccgt cgagcgcctt cattgcgggc ggtatttcga gaagccggag tcgtaccgga
     4081 cggttttctc caaacacttc acccgacgcg atccggaaca agaggctgta cgcgatttgt
     4141 cccgcgccac cggtcacggt aacgttgatt ggctgctgat gcatgtatac ccccatgtgt
     4201 tgctatccga cgtgtcagaa tcagcgtttt atgcgtccgt attccgtact ggaacataca
     4261 cttggaacgg tcgacattca aagctcccag caattcgaac cgagtcacgc gtccgcaatt
     4321 atggagcccg tgtgtgtgta actacctgcg tgttcgaggt actcattctc ccacgatgga
     4381 cgatttgcgc cccacaacgt actgaaaacg tgtcgcgtga gtccgagccc ccaaatacga
     4441 cgctggagcc gttcgcgttc agcgtaccat cgttttgccg cgccaatccg ttccgcatcc
     4501 cattcagcgc ggcgcatcac gtcggcgtgc gcttcgaaga gacgcagctt cgcttcgagt
     4561 tgttcgaagc gccatgaccg cggcgtaccc ctcgcgagac ggtcgaagtc acgacgagcg
     4621 gtgacgacgt cgcgttcgaa cggcgcgccc cacgaatcga gccaatcggc ccacaacaca
     4681 gcatccgcgc ccgatgcgcc gaaataccgc ccggcatacg cgcgcagcac gtcgttcgcc
     4741 gtgctgaac
//
LOCUS       DSCC01000382            2978 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022574, whole genome shotgun sequence.
ACCESSION   DSCC01000382 DSCC01000000
VERSION     DSCC01000382.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022574"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            28..393
                     /locus_tag="ENN56_04790"
     CDS             28..393
                     /locus_tag="ENN56_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74841.1"
                     /translation="MRFANGALGYHFGTWGARGTRLGYSFHAHCTEGMIEYRRSDPTF
                     RGERLIVHRGGTEEVVLSAENAKPTRDEMIHFLDCIDTGTQPITNGRDSLTGLRVIWR
                     LYDGEKRGVMADLRGVSER"
     gene            complement(570..1862)
                     /locus_tag="ENN56_04795"
     CDS             complement(570..1862)
                     /locus_tag="ENN56_04795"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS74842.1"
                     /translation="MFRRARRRIRVSEKLAIQGGTPVRTKPFPPGWHGVAEIGEKEEQ
                     AVLKVLREQRVFRFLGDQEKSETAQLEKAFCEATGQPYALAVNSGTSALICGLVGLEI
                     GPGDEVIVPGYTYIASAASIVAVGAVPVITEIDNSLTIDPDDIRKKITPYTKAIMLVH
                     MRGTPCRMDEIMAIAKEHNLAVIEDTAQADGGTYKGKVLGGIGDVGCFSFQMSKTITA
                     GEGGVVVMRDRKVYDRARMAHDSAFTFWRPGESEVDPIPGEGFRISEVSGAIALTQFG
                     RLPSIVERLRRVKYRIVDQIKDLRGIELQDMPDVPGDTSICLMFFGPTEAKAREYAAA
                     LNKEGIPCGTMFSKEIPDRHIYPNWTYVLEKRGRTPNWSPWHPSVYKGSVEYSKDMCP
                     KTLAYLGRTIHIGLSQKFTDEDADDIAKAIRKVVTALA"
     gene            complement(2581..>2978)
                     /locus_tag="ENN56_04800"
     CDS             complement(2581..>2978)
                     /locus_tag="ENN56_04800"
                     /inference="COORDINATES: protein motif:HMM:PF13561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS74843.1"
                     /translation="SVLNLFANAFEQRRSGFVAAISSAAGDRGRQSNYTYGAAKGGLT
                     RYLEGLRNRLYHSNVQVTTIKPGFMDTKMTQGMDLPKPLVASPEHAAELIWKAIKARK
                     DTVYVIGIWRLIMLAVRHVPEVIFKRMKM"
BASE COUNT          617 a          887 c          819 g          655 t
ORIGIN      
        1 gaaatggaag gcacgagcaa cgtatcaatg cggtttgcaa acggtgcgct cgggtatcac
       61 ttcggcacgt ggggagcgcg cggcacgcga ctcggctact cgtttcacgc tcactgcacc
      121 gaagggatga tcgagtatcg tcggtcagac cccacattcc gtggcgagcg gctcatcgtc
      181 caccgcggtg gcacggagga ggtcgtgctc agtgccgaga acgcgaaacc gacgcgcgac
      241 gagatgattc acttcctcga ttgtatagac actggaaccc agccgatcac aaacggccgc
      301 gatagcctta cgggtctccg cgttatctgg cggctctacg acggcgaaaa acgcggggta
      361 atggccgatc ttcgcggcgt ttccgaacgt tagcgaggag acacgcgcgt atatgacacc
      421 ggattgttat gcggattctt atggagctgc gatgggcgaa gacggatgtc ttcgccctaa
      481 cgaacgcggc acgaagtggc gcgttcgcac ataaaaaacg gggggcggac tcacgtccac
      541 ccccgttgca ctccactttc accgcgcgtt taggccagcg ccgtcaccac cttgcgtatc
      601 gccttcgcga tgtcgtcggc gtcctcatcg gtgaacttct ggctcaaacc gatatgaatc
      661 gttcgaccga ggtaggcgag cgtcttcggg cacatgtcct tgctgtattc aacgctgccc
      721 ttgtacacac tcggatgcca cgggctccag ttcggcgtcc gtccgcgctt ttcgagcaca
      781 tacgtccagt tcggatagat gtgccggtcg ggaatctcct tgctgaacat cgtaccgcag
      841 gggattcctt ccttattcag cgccgcggcg tactcgcgcg ccttcgcttc agtcgggccg
      901 aagaacatca ggcagatcga cgtatcacca ggcacgtccg gcatatcctg caactcgatg
      961 ccgcgaagat ccttgatctg atcgacgatg cggtacttca cgcgacgtaa gcgctcgacg
     1021 atcgacggca ggcggccgaa ttgcgtaagc gcgatcgcgc cggaaacttc gctgatccgg
     1081 aatccttcgc cgggaatcgg atcgacttcg ctttcgcccg ggcgccagaa agtgaacgcg
     1141 gaatcgtgtg ccatccgcgc gcggtcgtag accttcctgt cacgcatcac gacgacgcca
     1201 ccttcgcccg cggtgatcgt tttcgacatc tggaagctaa agcatccgac atcaccgata
     1261 ccaccgagga cctttccttt gtacgttccg ccgtcagcct gtgcggtatc ttcgatcacg
     1321 gcgaggttgt gttccttcgc gatagccatg atctcgtcca ttcgacacgg agttccgcgc
     1381 atatgcacca gcatgatcgc tttcgtatac ggcgtgatct tcttgcggat gtcgtccgga
     1441 tcgattgtga gcgagttgtc gatctcggtg atcaccggta ccgcaccgac cgcgacaatc
     1501 gaagcggccg acgcgatata cgtgtaaccc ggcacgatga cttcgtcgcc cggaccaatc
     1561 tcgagaccta cgagtccgca gatgagcgca ctcgtaccgc tgttgaccgc gagcgcgtac
     1621 ggttgccccg ttgcctcgca aaacgccttt tccaactgcg cggtctccga tttctcctga
     1681 tcgccaagga atcggaacac acgctgctcg cgcagcacct tgagaaccgc ctgctcttcc
     1741 ttctcaccga tttccgcgac accgtgccag cccggtggga acggctttgt acgaacaggg
     1801 gtaccacctt gaattgccag tttttcactc acgcgaatcc tccttctcgc tcgccgaaac
     1861 actcgcgagc tgcaacctca atgacgtaat gaaccagaac gcgctccgcg tccggtgccg
     1921 aagtattccc gtcgtcgatt ttcgtctacc gcggcggtca gaataccttg cagacgtgcg
     1981 acttagagaa cgagaatgag tataccagaa gcgctttcga ggacaaaggg agcgtattcg
     2041 tcaaaaaaaa atctataccg tgaccaccat cactgtaccc atggtcacac gtcagtatgt
     2101 ttgttctgga aacggtctca ttccgcaaca cactgtgtca gtgttaggta tatattccat
     2161 agtgactctt gacgcttgaa aatattgata tatactctct tttgcggcgg gggcgccatg
     2221 tcggttttca aggggtgatt gaaatggtga tggatgtcag gttcaacggg aggaagcgcg
     2281 cggaggatcg gtgtcgcgtc tttgtatgtc gtctcaccga cgggcgaatg gcggttgtac
     2341 ccgagtgcgt cgaagcggat atcgacttct accacaacct tgcggaagta aaacaacaaa
     2401 tagcgcttct catcccggat atccgcattc caatctatgg cgactatgaa cttgaaggtg
     2461 ttcgccgcgt gctgaatacc gcgatggcag ttcgtgccat gtaactccga ttcgcgcaac
     2521 cgaaacaaaa tgcccgcacc gaaaaaaacg atgcgggcgt tcgcgtgtat gcgggcgaac
     2581 ctacattttc attcgtttga agatcacttc cggcacgtga cgaaccgcaa gcatgatcaa
     2641 gcgccagatt ccgatcacgt acaccgtatc cttacgcgcc ttgatcgcct tccagatgag
     2701 ttccgcggca tgttccggtg acgcgacgag cggcttcgga agatccatcc cctgcgtcat
     2761 cttcgtatcc atgaatcccg gcttgatcgt cgttacctgc acgttgctgt ggtagagccg
     2821 gttgcgcagc ccttcgagat accgtgtgag gccgcctttt gccgcaccgt aggtgtagtt
     2881 cgactggcgt ccacgatctc ccgccgcgct ggaaatcgcc gcgacgaacc cgcttcggcg
     2941 ttgctcgaac gcgttcgcga agaggttgag cacgctga
//
LOCUS       DSCC01000383            1858 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1042605, whole genome shotgun sequence.
ACCESSION   DSCC01000383 DSCC01000000
VERSION     DSCC01000383.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1042605"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            82..1233
                     /locus_tag="ENN56_04805"
     CDS             82..1233
                     /locus_tag="ENN56_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74844.1"
                     /translation="MRAIIRQNGAPVFPLGMYEFPRDESEWKTWTDAGINLVRCGNRE
                     QLDIAHKYGVSVWVPVPMIVVDDASERRLIEMIETLVGHPALAVWEVPDEAIWRTICF
                     GDDRARRLWEEPEDRVVEVVRRRDELVKGLARGAKIIGERDPGRPIWLNEATPSDQDT
                     LARCLPWLDIVGFDFYPVPPSIERPMHEWGPLLDKYRRTAPRHELWAVQQAFSWSSLP
                     RHGAGKPPSYPNTDQVRFAAWQAIAHGATGLLWWGSSHEDRPAPFLADLMETVQELKK
                     VQPFLTAANIPSVRISANQRLRPSVLGVSGFVRLIEDADGRKRRMLALVNEDPWGHDV
                     ELTGLHDIGIPDPNEMRPVTPVSACPFVRLTDNWTVSLEGYEVRVYVTP"
     gene            1269..>1858
                     /locus_tag="ENN56_04810"
     CDS             1269..>1858
                     /locus_tag="ENN56_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74845.1"
                     /translation="MILRRKGRGLFALGMYEVPRDESEWKHWSESGINLVRCGSREEL
                     DTARDYGVFAWVPVPLIVRTDDDEAKLRNRIDELKDHPAIAVWEGPDEAVWFASRLKP
                     GTPTRLWIQDEDTVAKVETNLDELVHGYERGARIVRELDPNRAIWQNESGSNQSALAR
                     IMPYVDAIGFDGYPVPGRIEKPLQHLYGDTARYRAMA"
BASE COUNT          392 a          483 c          597 g          386 t
ORIGIN      
        1 cagatcgacg gcggttgggt gattgggatg gatgggtata gcgtgcgcgt cttcacgacg
       61 gcgtgacgaa aggataacgg tgtgcgcgcg attattcgac aaaacggcgc tcctgtcttt
      121 ccgctcggga tgtatgagtt tccacgtgac gagtccgaat ggaagacgtg gacggatgcg
      181 gggatcaacc tcgttcgttg tggaaatcga gagcagctcg atattgcaca caaatacggt
      241 gtttcggtat gggtgcccgt tccgatgatc gttgtcgatg atgcaagcga acgtcggctg
      301 atcgaaatga tcgaaacgct tgttggacat ccggcgctgg cggtgtggga agttcccgac
      361 gaggcgatct ggaggacgat ttgcttcgga gatgaccgcg cacgacgact ctgggaagaa
      421 cctgaagacc gcgtcgtcga agtggtgcga cggcgtgacg agctcgtcaa aggacttgcg
      481 cgcggcgcga agatcatcgg cgaacgtgat ccgggacgcc cgatctggct caacgaagcg
      541 acaccgagtg atcaagatac gctcgcgcgg tgtctcccgt ggcttgatat cgtcggcttc
      601 gacttctatc ccgtacctcc atcgattgag cgaccgatgc acgagtgggg tccactgctt
      661 gataagtacc gtcgaactgc gccgcgccac gaactgtggg cggtacagca agcgttttcg
      721 tggtcgagcc tgccgcgaca cggtgcaggg aagcctccgt cgtatccgaa taccgaccag
      781 gttcgcttcg cggcatggca ggcgatcgca cacggcgcaa cgggattgct ctggtggggc
      841 tcgtcacatg aggaccgtcc cgcgccgttc ctcgctgact tgatggaaac agtgcaagaa
      901 ctcaagaaag tccaaccgtt cctcaccgcc gcaaacattc catcggtgcg aatctcggcg
      961 aatcaacgtc ttcgtccgag tgtgttggga gtttccggat ttgtacgact gatcgaggat
     1021 gccgatggac ggaagcgccg gatgctcgcg ctcgtaaacg aagacccatg gggccacgac
     1081 gttgagctta ccggtctgca tgatatcggc attcccgatc caaacgagat gcggccagtg
     1141 acgccggtca gcgcatgtcc gttcgtccgt cttaccgaca actggacggt cagtctcgaa
     1201 gggtatgagg tgcgtgttta cgttactcca tgatcgtcag gtgtttcaca ctgtttggag
     1261 acagttgaat gatacttcgt cggaagggtc gtggattgtt cgcgctcgga atgtatgagg
     1321 tgccgcgcga tgaatcggaa tggaagcatt ggagcgaatc cggcatcaat ctcgtccggt
     1381 gcgggagtag agaagagctc gataccgcac gagattacgg tgtgtttgcg tgggtgcccg
     1441 tgccactcat cgttcgtacc gacgatgacg aggcgaagct ccgcaatcgc atcgatgagt
     1501 tgaaggatca tccggcgatc gctgtctggg aaggtccgga cgaggcggtg tggtttgcgt
     1561 cgcgactcaa acccggaacg ccgacgcgtc tgtggattca ggacgaagat acggttgcga
     1621 aggtggaaac gaacctcgac gaattggttc acggatacga acgcggagcg cggattgttc
     1681 gcgagctcga tccgaaccga gccatctggc agaacgaatc cggaagtaac cagtcggcgc
     1741 tcgctcgtat tatgccgtac gtagacgcca taggattcga cggctatccg gtaccggggc
     1801 gcatcgagaa gccgttgcaa catctgtacg gtgatacggc acgctaccgc gccatggc
//
LOCUS       DSCC01000384            3600 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1017424, whole genome shotgun sequence.
ACCESSION   DSCC01000384 DSCC01000000
VERSION     DSCC01000384.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1017424"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            90..344
                     /locus_tag="ENN56_04815"
     CDS             90..344
                     /locus_tag="ENN56_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74846.1"
                     /translation="MSPPFFSVGNDSNAPVTIPHLRSVIRPPGELTLNDLARLVHEPW
                     RANLTIKHGVTVRFPYMNTIFVKKVGEPPIAWGVCESAEE"
     gene            520..>3600
                     /locus_tag="ENN56_04820"
     CDS             520..>3600
                     /locus_tag="ENN56_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74847.1"
                     /translation="MAYLQRIHPARIIPLMIAALATVAAFSTAINAAPVTNGPRYLVD
                     ARGGGQAFVVAYDALEYSVDGGEAVAVLADDVGVVDLSAVTGPFAEIRLSSEAGYVVN
                     VANDADYTGDNTSNDQMILARGRDRYVDTEFLVYNHVPTVNNYNPAKIIVTAQEATAE
                     VTVSGLTGGMGTSVFEVELGQSTVLPLSESALLSITTDGAPVSVYYGILNDGAVEVVP
                     TTSGGFIGTEAYVATPEFVGVVATGTGFIHITERVINGGTEYDRTLLERSVITGEALY
                     VKTAHATTGLFDGVTTVKVSGTAPFYAFAGIGSAFSTYYGSTFIPAVPGSNGMRYRVQ
                     TLHPERASYLDVAPLDVGTTVTVSKTERGVVVGTVAPTSTDNGVYTYQLPASQTRQAN
                     VAAGYAKTVYLVQATGPIVVHERHAATVERGGIAISPDYEPQVPPSIGELDVTAIRVL
                     DEGNWIVDERLRVAFNAIDVNGDLQSVVLTDPTGATIDLTDHTTTIAGGVDVNTDWDG
                     IAYAYIPGTYTLTATDMLGMETVRVDSIRDVSVLDITAAQVNQPDNGSAGTELLPVYS
                     WISPRFEYASYYRFSVNADETFGDAGQVIIPFSAAKIAGKVSAAHASYATALNQDDLY
                     GWRVMGVASTLRWGDDFAVIGPVWTFSTSDAEDTSPPQFIRPPRVTNVTMDTVRVGWT
                     TNEPTTAVVNVFALDGTPQTSIDVVALSDENMATFVVGSTDALIWKLVVEITDQSGNT
                     AQSDELLFHIEEEPDTADPLFMAGPAHVSVTDVKATLTWTTNEPTAWVVHHWVRGVDP
                     ISQPDQISTKQDLETLQLSRQVDLTNLIPDTLYSYQVQVVDASSNVTYSRIQQFRTPA
                     GPDTRPPGIIGQPVVRDRTATSALITWRTDEGAIGKVRYGLADDPLNWSAGNPSAGLT
                     TNHEIRLTDLTSATQYFFAAHSIDASRNTSISDTLSFVTLSIPDTEGPRIRPAGQGGV
                     DKGTVVINRSDRMPVAKWNTNEDHTAVITVSSDPVVGEDGVFSNQAATVKVYSVT"
BASE COUNT          770 a         1050 c         1077 g          703 t
ORIGIN      
        1 accgacgtcg tccaccaacc gcaccgcgca accaccggtg cggtaacgag atcaccacat
       61 cacagaacat ggaaacggcg gaggcgatca tgtctccgcc gtttttttcg gtgggaaatg
      121 actcgaacgc acccgtcact attcctcacc tgcgttccgt cattcggcca ccgggcgaat
      181 tgactctcaa tgacttagca cggctcgtgc acgagccgtg gcgcgcgaat cttacgatca
      241 agcacggagt cacggtacgt tttccgtaca tgaacactat atttgtaaag aaagtcgggg
      301 aaccgccaat cgcgtgggga gtctgcgagt cggcggaaga gtaatctgat gtgtgtgcag
      361 caacgtcagc gttgtggcga acacgaacct cacatccgcg tctctttgac tgttgcactg
      421 ctattgcgga tatctcatcc gcataccgat acatcgttta tacggtactg caaccggttt
      481 ccgatggaga atcgagccgg taacctcggg gggcacatca tggcgtatct gcaacgaatc
      541 catccagcga gaatcattcc cttgatgatt gcggcgctcg cgactgtcgc cgcgttctca
      601 acggcgatca atgcggcacc agtgacgaat ggaccgcgct accttgtcga cgcgcgaggt
      661 ggcggacaag ccttcgtcgt tgcgtatgac gcactggaat actcggtcga cgggggcgaa
      721 gccgttgcag tactcgcgga cgatgtcggc gttgtcgatt tgtcggcggt gacgggaccg
      781 ttcgcggaaa tccgtctatc gagcgaggcc ggctatgtcg tgaacgtcgc gaacgatgcg
      841 gattacaccg gcgacaacac gtccaacgac cagatgattc tcgcacgcgg tcgtgatcgg
      901 tatgtggaca cggagttcct cgtctacaac cacgttccga cggtgaacaa ctacaatccc
      961 gcgaagatca tagtgaccgc gcaggaagca accgcggagg tcacagtcag cggtcttacg
     1021 ggcgggatgg gaacatcggt attcgaggtc gaactcggcc aatcgacggt gcttccgctt
     1081 tccgagtcgg cgctgctcag tataacgacc gacggtgcgc ccgtcagcgt gtattacggc
     1141 attctcaacg atggcgcggt tgaagtcgtt ccgacgacaa gcggcggatt tattggtacg
     1201 gaagcctacg ttgccacacc ggaattcgtc ggagtcgtcg cgacaggcac cggattcatc
     1261 catatcaccg agcgcgtcat aaacggcggc acggaatacg accgcactct cctcgagcgg
     1321 tcggtaataa ccggcgaagc gttgtatgtc aagaccgcgc acgccacgac ggggttgttc
     1381 gatggcgtta ccacggtgaa agtgagtggc actgcaccat tttatgcgtt cgccggaatc
     1441 ggttcggcgt tttccaccta ttacggctca acgttcatcc ctgcggtgcc cggctccaac
     1501 ggtatgcggt atcgcgtgca gacattgcac ccggaacgcg catcgtatct tgacgtcgcc
     1561 ccgctcgatg tcggaacgac ggtaaccgtt tccaagacgg agcgcggcgt cgtcgttggc
     1621 acggtggctc cgacaagcac ggacaacggt gtttacacct atcaactccc agcgagccag
     1681 acgcggcaag caaatgtcgc ggccgggtac gcgaagacgg tatacctcgt tcaggcgact
     1741 ggtccgattg tggttcacga acggcacgcg gcaacggttg agcgcggtgg tattgccatc
     1801 tcgcccgact acgagccgca ggttccgcca agcatcggtg aactcgacgt gacggcgata
     1861 cgcgttctcg atgaagggaa ttggattgtc gacgagcggc tccgcgtcgc gttcaatgca
     1921 atcgacgtca acggagactt gcagagcgtc gtgctcaccg atccgaccgg agcgacgatc
     1981 gatctcaccg accataccac cactatcgcc ggcggtgttg atgtaaacac cgattgggac
     2041 ggtatagcct acgcatacat tccgggaacg tatacgctga ccgcgaccga catgctcggt
     2101 atggagacgg ttcgcgtcga ctcgattcgc gatgtttccg tcctcgatat cacggctgcg
     2161 caggtgaacc agccggataa cggctcggca ggaaccgagt tgctcccggt atactcgtgg
     2221 atctcgccgc gtttcgagta cgcctcgtac taccggtttt cggtgaatgc cgatgagacg
     2281 tttggcgatg ccggacaggt aatcattccg ttcagtgcgg caaaaatcgc gggcaaggta
     2341 agcgccgcgc acgcgtcgta tgcgaccgca ttgaaccagg acgacctcta cggctggcgc
     2401 gtgatgggtg tggcgagcac gctgcgttgg ggtgacgatt tcgcggttat cggaccggta
     2461 tggacgttca gcacctccga cgccgaggac acgtctccac cgcagttcat cagaccgccg
     2521 cgcgtaacga acgtgaccat ggacaccgtt cgagtgggat ggacgacgaa tgagccgaca
     2581 acggccgtgg tgaatgtctt cgcgctcgac ggaacaccac aaacatccat cgatgtggtg
     2641 gcgttgagcg atgagaacat ggcgaccttc gtcgtggggt cgacggacgc gctgatctgg
     2701 aagctcgtcg tagagatcac cgatcagtcg gggaataccg cgcaaagtga cgagttgctg
     2761 ttccatatcg aagaagaacc ggatacggca gatccgctgt tcatggcggg tcccgcccac
     2821 gtatcggtca ccgacgtgaa agcgacgctc acgtggacca cgaatgagcc aaccgcatgg
     2881 gtggtccatc actgggttcg cggcgtcgat ccgatcagtc agcctgatca gatcagcacc
     2941 aagcaagacc ttgaaaccct gcagctcagc aggcaggtcg atctgacgaa tctgataccc
     3001 gatacgctct actcctatca ggtgcaggtg gtcgacgcgt cgagtaacgt cacctactcg
     3061 cgcatacagc agttccgcac acccgccggt ccagacacgc gtccgcctgg aatcatcggt
     3121 cagccggtcg tgcgagaccg gacggcgaca agcgcgctta tcacgtggag aaccgacgag
     3181 ggcgccatcg ggaaggttcg gtacgggctt gcagatgatc cgctcaattg gtccgccgga
     3241 aacccatcgg ccggactcac cacgaatcat gaaatccgcc tgacagacct tacctccgcc
     3301 acgcagtatt tcttcgcggc gcactccatc gacgcgtcgc ggaacacttc gatatcggat
     3361 acgctcagct tcgttacact ctcgattccc gatacggagg gcccgaggat tcgtccggcg
     3421 gggcaaggtg gcgtggataa gggcacggtt gtcatcaacc ggtcggatcg catgccggtc
     3481 gccaagtgga acacgaatga ggaccacacg gcggtgatta cggttagctc ggacccggtc
     3541 gtgggcgagg atggcgtctt cagcaatcaa gccgccacgg tcaaagtcta ttcggtgacg
//
LOCUS       DSCC01000385            1779 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1045147, whole genome shotgun sequence.
ACCESSION   DSCC01000385 DSCC01000000
VERSION     DSCC01000385.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1045147"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="ENN56_04825"
     CDS             complement(<1..1353)
                     /locus_tag="ENN56_04825"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74848.1"
                     /translation="MPRPNLLFIFTDEQRADTLAAYGNSRIDMPNLNMLAEKSTVFER
                     AYVSQPVCTPSRSTIMTGLTPHTNGCTRNNLPLDLEIPTIAEMLPDEYHCAYHGKWHL
                     GDELFVQHGFDEWISIEDGYNRHFRSDRDKSIRSDYHRWLIGHGFQPRRGETFGRDEA
                     TRLPEEYGKPSFLADQSIRFLRERADDGRTFALYINYLEPHMPFFGPRDDQHPLDEVS
                     IPPNFDNIPDETRPLRERLNYAHHYANGFGEYPLRTEADWRRLIANYWGLCSLVDTHT
                     GRILDALEETGLAENTIIVYTSDHGDMMGSHRMLTKSIMLEEAQRVPLLIHLPGQTEQ
                     RRISAPVGQIDLVPTLLELMDVETPKHVEGASLADATRTGDESSVTRDVFVEWTPGNT
                     NQSVAETAEAVIPEWKLAIASEEELRRRPAESVRGIVTRDKWKFNWSTVGDHELYNLE
                     E"
     gene            1538..>1779
                     /locus_tag="ENN56_04830"
     CDS             1538..>1779
                     /locus_tag="ENN56_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74849.1"
                     /translation="MTNGAMITSAYELAKAVHQIVSQFSEKKRDTIGQRMCETSVDVA
                     AKVQDALTTDDPVEQQEALRLAGLDSIALEILVRIGT"
BASE COUNT          358 a          478 c          529 g          414 t
ORIGIN      
        1 ctcttcgaga ttgtacagct cgtgatcgcc gacggtgctc caattgaact tccacttgtc
       61 gcgggtcaca ataccgcgaa cactttccgc cggacggcga cgaagttctt cttcggacgc
      121 aatggcgagc ttccactcgg gaatgactgc ttccgctgtt tcggcgacgg actggttcgt
      181 attccccggc gtccactcga caaaaacgtc gcgcgtgaca ctcgactcgt caccggtgcg
      241 ggtcgcatcg gcaaggcttg cgccttcgac gtgcttcggc gtttcgacat ccatgagttc
      301 gagtagcgtc gggacaaggt cgatctgccc taccggcgcg gatattcggc gctgctccgt
      361 ctgccccggc aggtggatga gcagcggtac acgctgtgct tcttcgagca taatgctctt
      421 cgtcaacatg cgatggctgc ccatcatgtc gccgtggtcc gacgtgtaga cgatgatcgt
      481 attttccgca agaccggttt cttcgagcgc gtccagtatg cggccggtat gcgtgtcgac
      541 gagactacag agtccccagt agtttgcgat aagacggcgc cagtctgctt ccgtgcggag
      601 cggatactca ccgaatccgt tagcgtaatg gtgggcgtaa ttgaggcgct cacgcagcgg
      661 ccgagtctca tccgggatgt tgtcgaaatt tggtggaatc gagacttcgt cgagtggatg
      721 ctgatcgtcg cgcggaccga agaacggcat gtgtggttcg aggtaattga tgtagagcgc
      781 gaacgtgcgc ccatcgtcgg cgcgctcccg aaggaaccga atcgactgat cggcaaggaa
      841 cgacggcttg ccgtactcct cggggagacg agtagcttca tcacgaccga acgtttcgcc
      901 tctgcgcggt tggaatccgt ggccgatcag ccaacggtgg tagtcggacc ggatggattt
      961 gtctcgatcg gaccggaaat gtcgattgta tccgtcctcg atgcttatcc actcgtcgaa
     1021 tccgtgctgt acgaaaagtt cgtcaccgag atgccatttc ccatggtacg cgcagtggta
     1081 ctcgtcgggc agcatctcgg cgatggttgg gatttcgaga tcaagtggga ggttgtttcg
     1141 tgtacagccg tttgtgtgcg gagtcaggcc cgtcatgatc gttgagcgag acggcgtgca
     1201 gaccggttga gagacatacg cgcgctcaaa caccgtgctc ttttcggcga gcatattcaa
     1261 attcggcatg tcgatacggc tgttaccgta cgcggcgagc gtatcggcgc gctgttcatc
     1321 ggtgaagatg aagagaagat tcggtcgcgg catggtaagc cctccgtttg cacaggcgtg
     1381 aacagatcct ccgagtgtcc tttaacattc ggacgttttt cgtcgcgggg cgagtctcga
     1441 aatacctatt ccgaacaata ttcctacgga cgattctggt atctgctcgt accctctcct
     1501 atattctttc tctacatcgc tttgtgggga gaaaaccatg acaaatggtg caatgattac
     1561 ctcggcatac gaactggcca aggctgttca tcagattgtg tcccagtttt cggaaaagaa
     1621 acgcgatacc atcggacagc gaatgtgcga aacgagcgtc gatgtcgcag cgaaggttca
     1681 ggacgcgctg acaacggacg atccggtcga gcagcaggaa gcgcttcggc ttgcaggact
     1741 cgatagtatc gcgctcgaaa tcctcgtacg gatcggtac
//
LOCUS       DSCC01000386            2015 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1038086, whole genome shotgun sequence.
ACCESSION   DSCC01000386 DSCC01000000
VERSION     DSCC01000386.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1038086"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="ENN56_04835"
     CDS             complement(<1..1063)
                     /locus_tag="ENN56_04835"
                     /inference="COORDINATES: protein motif:HMM:PF02894.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS74850.1"
                     /translation="MRTTEDHMTFDKPVINAGIIGLGMGATMFVAHDLPDSTIRVNAV
                     CDTNTELLDEVRDRYGVPFATMDWCELIGRSDIDVVGVFTPDALHADHVLAALDAGKH
                     VICTKPMTTSLADAVRILDAVRRTGRTFLVGQTCRFDRNRRAAKALVDKGAYGRLVYV
                     EATYNHDMRPVLDRTPWRWNMPQDLLFGGLCHPMDLAMWIGGRPREVSALASRGNLDS
                     RYPSGLDTNYVANIRFENGALGRVVGFYDFIHPEGMPYIELTVSGTRAASHEGRITWG
                     ADHTRQHSEDIAVHANEMDGDSGATDHRGEVAAYLRHFEHCLRSGREPSPGALEATRV
                     ISALEAVRRSAAENGAAVDV"
     gene            complement(1060..>2015)
                     /locus_tag="ENN56_04840"
     CDS             complement(1060..>2015)
                     /locus_tag="ENN56_04840"
                     /inference="COORDINATES: protein motif:HMM:PF15892.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74851.1"
                     /translation="CGLDNRLHLTYRWWHNIAIRGGPKSYRVTPSLGYQRTRPGMSWD
                     GFGHRYDPVPVWSDPASMIVPLVPNRYCQYGSSLAVDGLGRLHLGFHVYDQKYTERGH
                     TVGYMTSADGGETWTNARGDELTLPCGPTGADTLESDFDGDIETAPFDMRASNVAVTP
                     EGNPMIVAFHREDGHATLWESVPDDAKSGPVWRSTNLLPVLREHDEQRYFSMDGTVTV
                     DAAGNTYVAAQTVSELGHWGDGDSEVVLLLRPSGTQSWHVVDISEHTAYVPNWHPSLE
                     RIASPFQTMGTPYLLYQHGTKGTGCETYDNTEVRLVALTVE"
BASE COUNT          365 a          652 c          606 g          392 t
ORIGIN      
        1 gcacatcaac cgccgcaccg ttctccgcgg cggaacggcg taccgcttcg agcgcgctga
       61 tcacccgcgt cgcttcaagc gcacccggcg acggctcgcg gcccgatcga agacagtgtt
      121 cgaaatggcg aagatacgcc gccacttcgc cgcggtgatc ggttgcaccg ctatcgccgt
      181 ccatctcatt cgcgtgcacc gcgatatcct cactgtgttg ccgcgtatga tcagcacccc
      241 acgtaatgcg cccttcgtgg ctggcagctc gcgtaccgct caccgtgagc tcgatatacg
      301 gcatcccttc ggggtgaatg aagtcgtaga acccgacgac tcgtccgagc gcgccattct
      361 cgaagcggat gttcgcaaca tagttcgtat cgaggcccga tggatagcgc gagtcgagat
      421 tcccgcgact cgcaagagcc gatacctcgc ggggtcgccc cccaatccac atcgcgaggt
      481 ccatgggatg gcataaaccg ccgaagagaa ggtcctgcgg catattccag cgccacggtg
      541 ttcgatcaag tactggccgc atgtcgtggt tatacgttgc ctcgacgtag acgagtcgcc
      601 cgtacgcacc tttgtcgacg agcgctttcg ccgcgcgccg gttccgatcg aagcgacagg
      661 tctgaccgac gagaaacgtg cgaccggtgc gccggaccgc gtcgaggatt ctgacggcat
      721 cggcaagcga ggttgtcatg ggcttcgtgc agatcacgtg tttcccggca tcgagcgcgg
      781 cgagcacgtg gtcggcgtgg agtgcgtccg gtgtgaatac gcctacgaca tcgatatcgc
      841 tccgcccgat aagttcgcac caatccatcg ttgcgaacgg aacgccgtat cggtcgcgta
      901 cctcgtcgag caactcggta ttcgtgtcgc acacggcgtt cacgcgaatc gtcgaatcgg
      961 ggagatcgtg cgccacgaac atagtcgcgc ccattccgag tccgatgatt cccgcattga
     1021 taaccggttt gtcgaacgtc atatggtcct ccgtcgttct cattcaaccg tcagcgcgac
     1081 gagccggacc tcggtgttgt catacgtctc gcagccggtg cccttcgtgc cgtgttggta
     1141 gagcaaatac ggcgtgccca tggtctggaa cgggctcgcg atccgctcga gtgaggggtg
     1201 ccagttcggt acgtaggcgg tgtgctccga gatatcgaca acgtgccacg attgagtgcc
     1261 ggacggacga aggagcagca cgacctccga atcgccgtcg ccccaatgac caagctcgga
     1321 cacggtctgc gcggcgacat acgtgttccc ggcggcatcg accgtcaccg ttccgtccat
     1381 gctgaagtac cgctgctcgt cgtgctcccg cagcaccggc agcaggttcg tcgaacgcca
     1441 tacgggaccg cttttcgcgt cgtcgggcac tgattcccac aacgtcgcgt gcccgtcctc
     1501 gcgatggaac gcgacaatca tcggattccc ctcgggcgtc accgcgacgt tgctcgctcg
     1561 catgtcgaac ggcgccgttt caatgtcgcc gtcgaagtcg ctctcgagcg tatccgcgcc
     1621 ggtcggtcca cacggaagag tgagctcgtc gccgcgcgcg ttcgtccacg tctcgccgcc
     1681 gtccgcgctc gtcatgtagc cgacggtgtg gccacgctcg gtgtacttct ggtcgtacac
     1741 gtggaaaccg agatggagtc ggccgagtcc atcgacggca aggctgcttc catactggca
     1801 gtacctgttc ggcacgagtg gcactatcat tgacgcggga tcgctccaga ccggaaccgg
     1861 atcataccga tgtccaaatc cgtcccagct cattcccggg cgcgtgcgtt ggtatcccag
     1921 cgagggcgtg acgcgatacg acttcggacc gccacgtatg gcgatattgt gccaccagcg
     1981 atacgtcagg tgaagccgat tgtcgaggcc gcaca
//
LOCUS       DSCC01000387            1826 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043639, whole genome shotgun sequence.
ACCESSION   DSCC01000387 DSCC01000000
VERSION     DSCC01000387.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043639"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            290..1048
                     /locus_tag="ENN56_04845"
     CDS             290..1048
                     /locus_tag="ENN56_04845"
                     /inference="COORDINATES: protein motif:HMM:PF13472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDS74852.1"
                     /translation="MKRRDWHAKPFRRMVVLGESTVEGGGWIASAGERWADIVARLIN
                     ECQSQPMEYINRGIGANAISPRSPGYDASAKPSALERYRDDVTAQKPDLFLLCYGLND
                     MRAGMPIDEFIADMRTIIRDVREACNPLCVLTTVYYMTGWKSWAPFDRGSVELTEQYN
                     AAIAALADEEDCLLSDIWDAEGQADWLINPDGVHANKVGNLLIAHRVFETLANRCSGI
                     AGSVIERDRNSNWTRETSAARERGDDPYDPWWKK"
     gene            complement(1147..>1826)
                     /locus_tag="ENN56_04850"
     CDS             complement(1147..>1826)
                     /locus_tag="ENN56_04850"
                     /inference="COORDINATES: protein motif:HMM:PF16347.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HDS74853.1"
                     /translation="VFTSDHGSMLGSQGHYHKQQPWGESLSVPLIMRGPKRLRGTESD
                     LLFSLLDFAPTMLGLLGAPVPVRMQGRDLSPQIAHGAPYPRRVIYLAEFFCVDQAGGQ
                     GIKPWRGVKTNRCTYARTAEGPWVLYDDVADPFQLENLVDDPSVRDVRNRLDEELDMQ
                     MARFNDRIMTPDEALNHFGIRSAFNVRSEFLYHARNMGGVLDAVWPPSWSRDRIHAVE
                     EQGYSDV"
BASE COUNT          394 a          498 c          565 g          369 t
ORIGIN      
        1 tacagaaaca cgccgccgct ggatggtgta cgccgtcatt caactcatcg ttctttggtc
       61 gacagtcatc gccacgggca actttggcgg tcgcatcgtc ttcgactacg gagtcggaac
      121 ggcgatctac gacaccgcac cgaccgagga ggtttccgta cctgtctcaa cgcaattcga
      181 tacatcacga aaaagcaata gaacaaaata aggaccgtgt atgtcctgat tatcggtaat
      241 cgaccgtttg ttcggggaac gtgtattgtg gacggagagg agacgagaaa tgaagcgacg
      301 tgattggcat gcaaagccgt ttcggcgaat ggtcgtactc ggtgaaagca ccgtggaggg
      361 cggcggttgg attgcgtcag cgggtgagcg gtgggcggat atcgttgcgc ggttgatcaa
      421 cgaatgtcaa tcgcaaccga tggagtacat caatcgggga atcggtgcga acgcgatcag
      481 tccgcgaagt ccggggtatg acgcttcagc gaaaccgagt gcgttggagc ggtatcgtga
      541 cgacgtcacc gcgcagaagc ctgacctgtt cttgctctgc tacggactga acgacatgcg
      601 cgcgggaatg cccatcgacg agttcatcgc ggacatgcga accatcattc gcgacgtccg
      661 cgaagcgtgc aacccactct gtgtgctgac gacggtatac tacatgaccg gttggaaatc
      721 gtgggcgccg ttcgaccgcg ggtccgtgga gttgaccgag cagtacaatg ccgcgattgc
      781 tgcgttggcg gacgaggagg actgtctgct ctccgacatc tgggacgcgg aaggtcaagc
      841 tgactggctt attaacccgg acggtgtgca tgcgaataag gtcgggaacc ttctcatcgc
      901 ccaccgggtg ttcgaaacac tcgcgaaccg ttgcagcggc attgcaggct cagttatcga
      961 acgcgaccgg aattcgaatt ggacgcgcga aacatccgcc gcgagagaac ggggcgatga
     1021 cccgtacgat ccgtggtgga agaagtgagt tgagtaggcg gagtcgtacg ttgtgcacgg
     1081 cgtgatgatt ttgaaaacac gcgtgttcgg agggaggaac acgtcgcgcc agatagaaaa
     1141 ccgcgcctat acgtcgctat acccttgttc ctccacggcg tgtatccggt cccggctcca
     1201 actcggtggc caaaccgcgt cgagcacgcc gcccatgttg cgcgcgtggt agaggaactc
     1261 cgaccgtacg ttgaacgctg aacggattcc aaagtggttg agcgcctcat ccggtgtcat
     1321 gatacggtcg ttgaagcgtg ccatttgcat gtcgagctct tcgtcgaggc gattccggac
     1381 gtcgcgtact gacggatcgt cgacgaggtt ttcgagctga aatgggtccg cgacgtcgtc
     1441 gtagagcacc cacgggcctt ccgccgtccg cgcgtaggtg caacggttcg ttttaacgcc
     1501 gcgccacggc ttgattccct gtccgccagc ctgatcgacg cagaagaact ccgcaaggta
     1561 aatcacccta cgcggatacg gcgcaccgtg tgcgatttgc ggcgaaaggt ctcgcccctg
     1621 cattctcacg ggcacaggtg cgccaagcag accaagcatc gttggtgcga aatcgagcag
     1681 actgaacagg aggtccgact ccgtgccgcg cagtcgtttc ggcccccgca tgatgagcgg
     1741 gacgctgagc gattctcccc acggttgttg tttgtgataa tgtccttgcg aaccgagcat
     1801 cgaaccgtga tcactcgtaa acacga
//
LOCUS       DSCC01000388            2167 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034499, whole genome shotgun sequence.
ACCESSION   DSCC01000388 DSCC01000000
VERSION     DSCC01000388.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034499"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..399)
                     /locus_tag="ENN56_04855"
     CDS             complement(<1..399)
                     /locus_tag="ENN56_04855"
                     /inference="COORDINATES: protein motif:HMM:PF01171.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74854.1"
                     /translation="MLRRRRQRRTRCNASRTFGTSRVHRPNCSRESSHPRRCRRCRRT
                     LGRDTCRGACRAVSFGVGRRAVTDRPATRFQEQARWARQILLRSIAADVDADVIATAH
                     HADDQAETVLMHVLRGAGLSGLGGIAWGPEN"
     gene            complement(551..1930)
                     /locus_tag="ENN56_04860"
     CDS             complement(551..1930)
                     /locus_tag="ENN56_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74855.1"
                     /translation="MTLTVYPSAQDVVSALDWHRIVTQRWFRRKDATYVRFDLIDGRP
                     VFRGRSLHGWWLLIHATTKNDSADYQLPVLLTTNPCVRSSVFGTCRKGADTLQIVDGI
                     GDASMLASLLTTCDPEFEHGRVEARFQGDPLQIPDDVSVLSADSTNSLVRIANGHVLK
                     IYRVLDAGGTREVDFLEALAQPRVASVPTLHASVEYRTGTDRRALALLQEWIDDAEDA
                     WSWLTKLLANGPQNITDDVVAMADAVGSVHAGLRRRGTRPWSKTDTKDLLSRVTAGLA
                     RAELYIPDDAKLRKMLAHVRDYVEADFDSESGCLQAVHGDLHLGQLLRQNAAGDSVRY
                     YIIDFEGEPLAPPDERCAWHSPLKDVAGMLRSFDYAVGVARKAGAPGDDAVWNVWRAE
                     CERTFLARYSIHSDDTKVDTDTLLALFVIEKVLYEIAYEATNRPNWIDVPLGGLSRVV
                     DRLRTLLNQ"
BASE COUNT          459 a          637 c          631 g          440 t
ORIGIN      
        1 attttcagga ccccacgcga tcccacccaa tcccgaaagg cccgcgccgc gcagtacgtg
       61 catcagcacc gtttccgcct ggtcgtcggc gtggtgcgcg gtcgcaatca cgtctgcgtc
      121 aacatcggcg gcaatgctgc ggagaaggat ttggcgcgcc caacgtgcct gctcttggaa
      181 ccgcgtcgcc ggtcggtcag tgacggcacg tcgaccgacc ccaaatgaca cggcacggca
      241 agcgcctcgg caagtgtcgc gacccaacgt gcgtcggcat cggcgacatc gccgcggatg
      301 cgatgattca cgtgagcaat tcggacggtg aacccgagat gttccgaaag tacgtgaagc
      361 gttacagcga gtgcgacgct gtcggcgcct ccggagcatg cgacgagtac cgtctgctcg
      421 gcggaaatca accggcgttc gcgaatcgtc cgacgagctg cttcgataaa cggatcggat
      481 cgcttcggta gcggaaggcg cgcaatatcg ggcgcacggt gtggttttgt atgcgacgcc
      541 attggagagc tcattgattc aagagtgtgc gcagtcgatc cactacgcga ctcagtccgc
      601 cgagcggaac gtcaatccaa ttcggacggt ttgtcgcttc atacgcgatc tcatacaaca
      661 ctttttcgat gacgaagagt gcgagcaacg tgtccgtatc gaccttcgta tcgtctgaat
      721 gtatcgagta ccgcgcgagg aacgttcgtt cacattcagc gcgccacacg ttccataccg
      781 catcatcgcc cggtgcgccc gcctttcgcg ccacaccaac cgcgtagtcg aacgatcgca
      841 gcattcccgc cacgtctttc agcggcgaat gccacgcgca ccgttcatcc ggtggagcaa
      901 gcggctcacc ttcgaagtca atgatgtaat accgtacgga gtcacctgcg gcgttctgac
      961 gaaggagctg accgaggtgt aaatcgccgt ggacggcctg aaggcagcca ctctccgaat
     1021 cgaaatcggc ctctacgtaa tcccgtacat gtgcaagcat tttgcgtagc ttcgcatcat
     1081 ccgggatata caattctgca cgtgcgagtc ccgcagttac tcgggagagg aggtctttcg
     1141 tatcagtttt tgaccacggt cgcgtaccac ggcgacgcag accagcgtga acgctgccga
     1201 ccgcgtcagc catcgcaaca acgtcatcgg ttatgttttg cgggccgttc gcgaggagtt
     1261 tcgtcagcca cgaccatgcg tcctcagcgt catcgatcca ttcctgaaga agcgcgagtg
     1321 cgcgtcggtc ggtgccggtt cggtattcca cgctcgcgtg aagcgtcggg acgctcgcaa
     1381 cccgtggctg cgcaagtgct tcgaggaaat ctacttcgcg cgtaccgcct gcgtccaaga
     1441 ctcggtaaat cttgaggacg tgcccgttcg cgattcgaac gagcgagttc gttgagtccg
     1501 ccgacaatac cgaaacatcg tcgggaatct gcagaggatc gccctggaat cgcgcttcga
     1561 cgcgaccatg ctcgaactcc gggtcgcacg tcgtcagaag gcttgcgagc atcgaagcat
     1621 caccgatgcc atcgacgatc tggagtgtat cggcaccttt ccgacacgtt ccgaatacgg
     1681 aactacgaac gcacggattc gtcgtgagta gcacggggag ttgatagtcc gctgagtcgt
     1741 ttttcgtcgt tgcgtggatg agcagccacc atccatggag agagcgacct cggaatacgg
     1801 gccgaccgtc gatgaggtcg aatcgaacgt acgtggcgtc tttccggcgg aaccaccgtt
     1861 gcgttacaat gcgatgccaa tccagagcgg atacgacatc ctgcgccgac ggatagacgg
     1921 tgagtgtcat gccgtgtacc acgttgactg tacgatcaac gggacgatga gtgtttgcgt
     1981 cgtgagctct tcctgccaaa ctgacgttgg agcagccatc cgatcacggt catcgacgcg
     2041 ccaagaatga tgtacgtctg gttctggcca atatccaatt cgagatagaa gatggatacg
     2101 acgatcaccg caccgatgag catgatgacg agtccgatta cggcgacaaa ggggttatat
     2161 tgacgtc
//
LOCUS       DSCC01000389            3085 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021492, whole genome shotgun sequence.
ACCESSION   DSCC01000389 DSCC01000000
VERSION     DSCC01000389.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021492"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>3085)
                     /locus_tag="ENN56_04865"
     CDS             complement(<1..>3085)
                     /locus_tag="ENN56_04865"
                     /inference="COORDINATES: protein motif:HMM:PF02225.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74856.1"
                     /translation="TLALPDDTPEEMLYYSLSIGMGDVLVRGGNRDTTTLRLEPGISG
                     ELVYAGNGDYGGFNNRDVVGSIAVMDFTSGTNFINARILGAKAVIFVDDGMVNRSEAE
                     RKFLNVPADVSRFWVEGEVAERVKALGSVPGNRVTLTSRMEWTAVTAYNIWGTIPGTR
                     TNTADSTVVLQAYYDAMSVVPRRAIGAENAVSMATLLSVAESFSVHPPEIPFTFLATS
                     GHFTNLSGINEWLYRHVRKESRFLENIPEDQRIEFGLFIGLDLSSENDQIGAFAQGTY
                     YSGWNTDARRTNMHTGYGRAFLRYSAELFPGQEVSEAYPQYADGIAPAKRTWRTYTPT
                     MLAFDHEPVNYVGLSAFSFATTNDARIRVDTPVDTLGALNTANALTQARTIAGLMYRA
                     ARDQGFFQDTKIQPKDTQRELTGRILQFDRRKSFIPNSPIPEAVVTVAYGQKTYTGVR
                     GLNVTLTNGFGEDGRDWLRYTLGGESGIGQSPDDPGRFRFHMLAPHVGAVTVKAYKIA
                     DSGRIVMAPDLGEEGDKTYPMAVGTSSAVIQTLQVLFEARAMTMFEIVDSRYLTALDM
                     LNLRGPDGSELRSFGMEVVPLQSRAEGNVENAAVVFARPGTRVKVMMSTSLFGIKYLL
                     INAPQHLFTNPITPRQANPEAQEIAEGIGYLIDEGVITKTPQRVAQDMWAIDDVRMKT
                     LQRYGVVNQRVLDLHTQAREALLAADVAFEARDYGTWIREARHAWGLEARAYPDVKGT
                     AVDTVQGVVFYFALLLPFAYFMERMIIGAVDIRRQLLGFASIFVGVFVILQFVHPAFK
                     LSSSPYIIFLGFVIFALGTVVTVIVVGRFNDELKKMKQSQSGMHEADVGRIQATAAAI
                     SLGINNLRKRPLRTTLTAVTIAILTFSVLSFTSVVTSLQFYKINRSNEPTYDGMLIRD
                     RNWMGLQPSVLEYVATAFHDKALITPRSWLMAQVRADKAFIDFVNLDNHDRSFAGGLL
                     GVVPEEDRVTNISAMLTAGRWFNPGERGVVILPERMASIVNISPDDVGKVTIDMLG"
BASE COUNT          657 a          909 c          875 g          644 t
ORIGIN      
        1 cccaacatgt cgatggtaac cttaccgacg tcatccgggc tgatgttcac gatcgatgcc
       61 atacgctccg gtagaatcac tacgccacgc tcgcccgggt tgaaccaccg gcccgccgta
      121 agcatggcgg aaatgttcgt gacgcgatcc tcttccggca cgacgccgag cagaccgccg
      181 gcgaagctcc ggtcgtgatt gtcgaggttg acgaagtcga tgaacgcctt atccgcacgc
      241 acctgcgcca tgagccagct acgcggcgtg atgagtgctt tgtcgtggaa cgccgtcgcg
      301 acgtattcga gaacgctggg ctggagaccc atccagttac gatctcgaat caacataccg
      361 tcgtacgtcg gttcgttact gcgattgatc ttgtagaatt gaagcgacgt gacaacggac
      421 gtaaacgaga gcacggagaa ggtcagaatc gcgatggtca ccgcggtcag cgtcgtgcgt
      481 aacgggcgct tccgtaggtt gttgattccg aggctgattg cggccgccgt cgcttgaatt
      541 cgcccgacat cggcttcatg cataccggac tggctctgtt tcatcttctt cagctcgtcg
      601 ttgaaccgcc ctacgacgat cacggtgaca accgtgccaa gcgcgaatat cacgaatccg
      661 aggaagatga tgtacggcga ggaactcagc ttgaacgccg gatgcacgaa ttggaggata
      721 acgaacacac cgacgaagat cgacgcgaac ccaagaagct gtcggcggat atccacggcg
      781 ccgataatca tacgctccat gaagtatgca aacgggagca gtaacgcaaa gtagaacacc
      841 acaccttgca ccgtatcgac cgccgttccc ttaacatcgg gatacgcgcg ggcttccagg
      901 ccccatgcgt gacgagcctc gcgaatccac gtgccgtaat cgcgcgcttc aaaggctacg
      961 tcggcggcaa ggagcgcttc gcgcgcctgt gtatggagat cgagcacgcg ctggtttacg
     1021 acgccgtacc gctgaagcgt cttcatgcgc acgtcgtcga tggcccacat gtcttgggca
     1081 acacgctgcg gtgtcttcgt gataacgcct tcgtcgatga ggtagccgat gccttccgcg
     1141 atttcctgtg cttccggatt cgcttgacgc ggagtaatcg gattcgtgaa gagatgttgc
     1201 ggcgcgttga tgaggaggta tttgataccg aagaggctcg tgctcatcat aacctttacg
     1261 cgcgttccgg ggcgtgcgaa gaccaccgcc gcattctcaa cgtttccttc ggcgcggctc
     1321 tgcaacggaa cgacttccat tccgaagcta cgcaactccg agccatcggg accgcggaga
     1381 ttcaacatat ccagcgcggt gaggtagcgg ctgtcgacga tttcgaacat cgtcatcgcg
     1441 cgcgcttcga atagcacctg caacgtctgg attaccgccg acgacgttcc gacggccatc
     1501 ggatacgtct tgtcaccctc ttcaccgagg tcgggcgcca taacaatgcg accgctgtcg
     1561 gcaattttgt acgcttttac cgtcacagcc ccgacatgcg gtgcgagcat atgaaagcgg
     1621 aatcgacccg gatcatccgg gctctgaccg atgccgctct cgccgccgag tgtgtagcga
     1681 agccagtcgc gaccatcctc gccaaagccg ttcgtcagcg ttacgttcaa cccgcgtaca
     1741 ccggtatacg tcttctgccc gtaggcgacc gtaacgacgg cttcaggaat cggtgagttg
     1801 ggaataaacg atttgcggcg atcgaactgg aggatacggc cggtaagttc gcgttgcgtg
     1861 tcctttggtt ggatcttggt atcctggaag aacccttggt cgcgcgctgc acgatacatg
     1921 aggccggcaa tggtgcgcgc ctgcgtcaac gcgttcgcag tattcaacgc gccaagcgta
     1981 tcaacgggag tgtcaacgcg aatacgcgcg tcatttgtcg ttgcgaagct gaatgcgctc
     2041 aagccgacgt agttgaccgg ctcgtggtcg aaagcaagca tggtcggcgt gtaggtacgc
     2101 catgtgcgct tcgccggggc gataccgtcg gcgtactgcg gatacgcttc gcttacttcc
     2161 tgaccgggga agagctctgc cgaataccgc aaaaacgccc ggccgtagcc ggtatgcatg
     2221 ttcgtccgac gcgcgtcggt attccagccg gagtaatacg tgccctgcgc aaacgcaccg
     2281 atctggtcgt tttcacttga gagatcgaga ccgataaaca acccgaactc gatgcgctgg
     2341 tcttccggaa tgttctcgag gaagcggctt tctttccgaa cgtgacggta gagccattcg
     2401 ttgatacccg aaaggttcgt aaagtgtccc gacgtcgcga ggaacgtaaa cggtatctcc
     2461 ggtggatgaa cactgaagga ttccgccacg ctcaagaggg tcgccatact gactgcgttt
     2521 tcggcaccaa tagcgcgacg tggcacgaca gacatcgcgt cataatacgc ctgcaacacc
     2581 accgtcgaat cagcggtatt cgtccgagtc cccgggatcg ttccccagat gttgtaggcc
     2641 gttacggcgg tccactccat gcgactcgtc aacgtcacgc ggttacctgg aacgctcccg
     2701 agcgctttga ctcgttcggc gacctctcct tcgacccaga atcgcgacac atctgctgga
     2761 acgtttaaga acttacgctc ggcctcagag cggttaacca tgccgtcatc gacgaaaata
     2821 acggccttcg cgccgagaat gcgcgcgttg atgaaattcg tcccgctcgt aaaatccata
     2881 actgcgatgg aaccgacaac atcgcggttg ttaaagccgc cgtagtcgcc gtttccggcg
     2941 tacacgagtt cgccgctgat tcccggctct aaccgaagcg ttgtcgtatc acgatttcca
     3001 ccgcgcacga gaacgtcgcc cattcctatc gagagcgaat aatagagcat ctcttctgga
     3061 gtatcgtcgg ggagcgcaag tgtcg
//
LOCUS       DSCC01000390            2181 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1034226, whole genome shotgun sequence.
ACCESSION   DSCC01000390 DSCC01000000
VERSION     DSCC01000390.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1034226"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            1678..>2181
                     /locus_tag="ENN56_04870"
     CDS             1678..>2181
                     /locus_tag="ENN56_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS74857.1"
                     /translation="MGETIHEPARELPVISDVDICVLGGSATGVFAAVRAARLGAKVA
                     IVERLNRFGGMATAALVNVWHSLYDEVHERKIIAGLTHETMDRLATRNAVENIDRNPN
                     QAFIFNSAELATELDQLVVESGIEPHLHTAFAAPLVEDGNLVGIAVENKSGRGAIRAR
                     MFVDATGD"
BASE COUNT          468 a          614 c          690 g          409 t
ORIGIN      
        1 gacggcgact atttcagagt cggcaaggcg gagtacgaga tcgtggtcat cccacccggc
       61 gtgacgcttc gttcgagcac gttgcgcctt ctctcccagt ggatggaaga gggcggaccc
      121 gtaattgcgg tcaaaccgat tccccgccac atcgatggac ttgcgccgga ccgtgagttg
      181 ggcgaggacg cgtgggacgt tctgcgcgag gtgatcatcg tcgagaagaa tcaggaagat
      241 tttatcggcg cattggtcga ccttgtctca ccgagcgtgc aagtgcttac accggaggga
      301 ttcccggtcg cgccggtctg ggtgcatgag cggtatgtgg aggcgaacga cgagaacgat
      361 accgcgatga gcatctactt cctcgcgaac accgataaca cgcgcggata cgcctgcaca
      421 atcgtgctgc gcggctccgg tgacgtggaa ttgtgggatc cgcggaccgg cgaaatcgag
      481 ccgattggag ttgaacgggc cggcactcac gtcatcatcg aagactacat cgacgctgca
      541 ggttctcgcc ttttcgtgca acgacacggc gaggaattgg agttgagtga agccgaagcg
      601 gaccgaatcg cggcggaaaa gcttgccgaa gagcaggagc atttacacga tcacagtgcc
      661 gaatcaatgg acgagatcga tttggagggc acttggcggg tgcggcggca cgatccaaac
      721 gctattaccc ttgatcttgc gtactggcgg ttgatcgagc ccgacggcgc gaatgccgat
      781 gacgggaagc gcgaactccc aatcctcgat actgacgatg agaacgacga ggtctggaac
      841 ggtccggatc cgcttcatcg tccgttggga ccggtgcgga agcacacggg cgacttcgaa
      901 ctcgccttca cgtttgtcgt tgataccgtg ccggaaggcg acctcttcct cgttctggaa
      961 acgccagagg cgtttgagat cacgctgaac gacacaccgg tgccggatga cgatgccggg
     1021 tggtgggtcg atacaacgtt ccgcaaacgt cgcatcaccg gcatggtcga atcgggcgag
     1081 aacaccatcg ttctgcgagg tagcgccacg ccgacgatcg agttggaaac ggtgtatgtg
     1141 attggaaact tcggcgtctg gaccgaagac ctgcaggact tcaccatcgc cggcgaatcg
     1201 gtcgactccc ccggcggtga tctcgtcgaa cagggattcc ccttctacgc gggcacggtc
     1261 tcgcttgaac aacgaatcga actgacactg aacggccaca catcggcgcg cctcgtcatc
     1321 gatgggcttg ccgctaccgt cgccgatgtg ttcgtgaacg gcgagaacgc cggtcagatc
     1381 gcgtgggaac cccacgaggt agaaatcgga ccgtatctga aggatggccg caacatcatc
     1441 cggatcgact tggtgaacac gctgcgcaat ctgctcggac cgcatcatca tcggtacggc
     1501 gagctcctcg gcgtaggtcc gggatcgttc tccgacgaag gcaactggac cgacatctac
     1561 cagttcgtgc cgttcggggt gagcggcgtg cgtatcgtcg tatcagaata atcgtgggtt
     1621 acggggcgta atatcccatc aaaagggata ttacgccttt ctttaggaag gcacgcaatg
     1681 ggcgaaacga ttcatgaacc cgcacgcgaa ttaccggtaa tctccgatgt cgacatctgc
     1741 gttctcggcg gaagcgccac gggcgtcttc gccgcggtac gggcggcgcg actcggtgcg
     1801 aaggtagcga ttgtcgagcg actgaaccgt tttggcggca tggcaacggc tgcactggtc
     1861 aacgtgtggc acagtctcta cgacgaggtg catgagcgga agatcatcgc cggcctcacg
     1921 cacgagacga tggaccggtt ggcgacacga aacgccgtgg agaacatcga tcggaatccg
     1981 aatcaggcgt ttatcttcaa cagcgcagag ctggcgacgg agctcgacca actcgtcgtc
     2041 gagtcgggca ttgaacctca cctgcatacc gcgttcgccg caccgctcgt ggaagacggc
     2101 aaccttgtcg gcatcgcggt ggaaaacaag tccggacgcg gagcgatacg cgcgcgaatg
     2161 ttcgtggacg ccaccggcga c
//
LOCUS       DSCC01000391            1916 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1040831, whole genome shotgun sequence.
ACCESSION   DSCC01000391 DSCC01000000
VERSION     DSCC01000391.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1040831"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            881..1465
                     /gene="nuoE"
                     /locus_tag="ENN56_04875"
     CDS             881..1465
                     /gene="nuoE"
                     /locus_tag="ENN56_04875"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDS74858.1"
                     /translation="MISPSDISSIKRLDNGHKNRDIDTEVVDRILSGYASAGASSLIP
                     ILQDLQESFGYLPEVAIDLVGRALDISTARIYGVATFYNQFRLNPLGKHVIRVCRGTA
                     CHVKGSLALLETLESELGIKAGGTTKDGMFSVETVACLGACSIAPAVMVDDKFSGRLT
                     VKQMPRLVKQIQRDEAKLAKSNGGDAGSDTTDTE"
     gene            1565..>1916
                     /locus_tag="ENN56_04880"
     CDS             1565..>1916
                     /locus_tag="ENN56_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HDS74859.1"
                     /translation="MYTLLEPCCSECWHSAEKPCPEVIDCIVDGPRCHTNKKCTALRR
                     ERLAVAAGEKLERTTIRIGMGTCGMASGAQEVYDTFAAEIAEKGLDADIIPVGCYGYC
                     EKEALVDIQRPGHPR"
BASE COUNT          404 a          533 c          574 g          405 t
ORIGIN      
        1 acgcgaaacg atccgggcga ccgccgcaag cggatggaag cgatctactg ccgactggtt
       61 cgagaggcgc tcgacgccgg tacaatgccg cttcttgtca aggaagcgaa tctaccgagt
      121 gtaatgtcga cactgttccg tccgccgacc ttgatcgtcg ccgatgtcga gcatttcgac
      181 cgactcgctc gcatggttcc cgaagacatt ccgttgacga cgctcagtgt gcttgcggcg
      241 cgcgagaaag gcaatctccg agggtttgtt gagggaatcg tgtcgctcag gcggcttcgg
      301 cccggtgatc gcgtgttgat cgtcgaagcg tgcgcgtatc gtccgctcag tgaacacgcg
      361 gactacctct cattgccaac gattctgcgg acgtttatcg gaggtccgct tcgtattacg
      421 actgcgagtg gaccggacgc gtttcccgag tcgctgcgtg ggtatcaact cgtcattcat
      481 tgcgcggcgt gctgcttgga tcggccggag atgcttcgcc gacaatcgct cgcgcaacga
      541 cagggagtcc ctctggtgaa ccacgcgctg ttgcagacgc acctatcaca ctccctgagt
      601 cgggccttgg cacccctgcg acagaatggg gcgctcctgg acgatttgga gagcatcgtc
      661 gacgatcatc gcgttcgcgt actcgcctaa tacggccatc tgagcgatga aagtgtaaat
      721 atcacactaa cccgatctcc attcgtcgga cgggtacgcc ctttgcacca tagcatagcg
      781 caagacgtcc ttggcatgtc tgcgcttcac accggtaagc atagaccagc agtagacatg
      841 catacatgaa ccgtcagtca ttttacactg taggaccgca atgatctcac catctgacat
      901 cagctcgatc aaacggctcg acaatgggca caagaatcgc gacatcgata ccgaagtcgt
      961 tgaccgcatt cttagcggat atgcatccgc tggagcgagc tcactcatcc cgatccttca
     1021 ggatttgcag gagagtttcg gctacttgcc ggaggtcgcg atcgacctcg tcggtcgcgc
     1081 gctcgacata tcgacggcgc gcatctacgg tgtcgcgacg ttctacaacc agtttcgctt
     1141 gaatccgctt ggtaagcacg tgatacgtgt ctgccgcggt acggcgtgcc acgtaaaagg
     1201 ttcgttggcg ctgctcgaaa cgcttgagag tgagctgggc atcaaagccg gtggtacgac
     1261 caaggatggg atgttcagtg ttgagaccgt tgcgtgcctt ggtgcgtgca gcatcgctcc
     1321 agcggtgatg gttgatgaca agttctccgg tcgactgacg gtcaagcaga tgccgcgtct
     1381 tgtcaaacag attcagcgcg atgaggccaa gctggcgaag tcgaacggtg gcgacgcagg
     1441 ttcggatacg acggatacgg aataatacgg cactcacccg gctcgatgaa tcgggaattg
     1501 ttcgagatat cgcggagagc gagcggctac aacgtgcgcg cgaaccccgc ttggaaggag
     1561 agcaatgtat acgttattgg agccctgctg ttcggagtgt tggcactctg cggaaaagcc
     1621 gtgccccgaa gtgatcgatt gcatcgtgga cggcccgcgt tgtcatacga acaagaaatg
     1681 cactgcatta cgccgtgaac ggctcgctgt agctgccggc gaaaagcttg agcgcacgac
     1741 gattcgtatt ggaatgggca cctgcggtat ggcatccggc gcgcaggaag tgtacgacac
     1801 gtttgccgcc gaaatcgcag agaaggggct cgacgccgac atcataccgg ttggttgcta
     1861 cggctactgc gaaaaggagg cgctggtcga cattcaacgg cccggtcatc ctcgtg
//
LOCUS       DSCC01000392            1701 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047963, whole genome shotgun sequence.
ACCESSION   DSCC01000392 DSCC01000000
VERSION     DSCC01000392.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047963"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            149..610
                     /gene="msrA"
                     /locus_tag="ENN56_04885"
     CDS             149..610
                     /gene="msrA"
                     /locus_tag="ENN56_04885"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDS74860.1"
                     /translation="MEPSIAIFAAGCFWGVEKTFRNVDGVLATTVGYIGGHVDHPSYE
                     HVCTGVTDHAEAVRINYDPARVSYEELLDVFWSAHDPTQLNRQGPDIGRQYRSAIFPQ
                     SDEQRSVAEASLAKQQAKTKKPIATQIESSTTFWPAEDYHQQYLEKRGRTS"
     gene            788..>1701
                     /locus_tag="ENN56_04890"
     CDS             788..>1701
                     /locus_tag="ENN56_04890"
                     /inference="COORDINATES: protein motif:HMM:PF02837.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74861.1"
                     /translation="MTDYNDPRVVQRNKEPGHVARIPFPDTHSAAIGARGASPWFRLL
                     NGVWRFNLAPVPESAPAGFEAVEFDDSGDGWADIEVPSNWQLKGYDKPIYTNVKMPFP
                     KELYPSWPADDNPTGSYRTVFEVPEGWAERRTFLLFEGVDSAFHLWVNGREVGYSQGS
                     RLPAEFDITDYVRAGENTLAVRVYRWSDGSWLEDQDYWWLSGIYRDVVLYSLPAVNIR
                     DFTVRTPLDSAYHDARLQVTALIRNDSDEPQNVELGVTLLDRDGHEVSLDHAEDATGL
                     RPGQESDIAFDLPAENPAKWSDETLYLYT"
BASE COUNT          350 a          518 c          494 g          339 t
ORIGIN      
        1 gtgcacgccg gtgacccgtg gcagcggcag gagttcgcgc tgctctttct tgtcccgttc
       61 ctgacgttag tgtttacggg accggggcac tactcgattg acggtcttat tcgttggcgg
      121 acgccggaac tggatcgcag aggataatat ggaaccgagc atcgcgattt ttgccgccgg
      181 atgcttctgg ggcgttgaaa agacgttccg caacgtcgat ggcgttctcg caacgaccgt
      241 cggctatatc ggcggacacg tggatcaccc gtcatacgaa cacgtatgca ccggtgtgac
      301 ggatcacgcg gaagctgtcc gcatcaatta cgatccggct cgcgtttcct acgaggaact
      361 gctcgacgtt ttctggagcg cacacgatcc gacgcagctg aatcggcaag ggccggatat
      421 cggcaggcag tatcggagcg ctatcttccc gcaatccgat gaacagcgca gcgtcgcaga
      481 agcctccctc gcgaaacagc aagcgaagac gaagaagccg attgcgactc agatcgaatc
      541 atcgacgaca ttttggcccg cggaagacta ccaccagcag tatctggaaa agcgcggtcg
      601 tacgtcgtaa cgcaacaacc ttagtacctc ctcatggaaa aacgcgcgcg gttttttcgg
      661 tatcgacttg gaacgcgcgc ggtttttccc gtacttgatc attctatcgt tccacaacac
      721 atgctctacg gcaggagcgc gttcaaactc gatcgatcac tgtctttcca ctccgaggtg
      781 acgacctatg accgattaca acgatccgcg cgtcgttcaa cgcaacaagg aaccgggcca
      841 cgttgcgcgc atccccttcc ccgatacgca cagcgcggct atcggcgctc gcggtgcgtc
      901 gccgtggttc cggttgctca acggcgtgtg gcggttcaat ctcgcacccg ttcccgaatc
      961 ggcgcctgcg ggattcgaag cggtagagtt tgacgactcc ggcgacggct gggcggatat
     1021 cgaagtgcca tcgaactggc agctcaaggg atacgacaag cccatctaca cgaacgtcaa
     1081 gatgccattc ccgaaggagc tctatccgag ctggccggcg gacgacaacc cgaccggctc
     1141 gtatcgcacc gtgttcgaag tgcctgaagg atgggccgag cggcgcacgt tcctcctctt
     1201 cgaaggcgtc gattcggcgt ttcatctgtg ggtgaatgga cgcgaggtcg gctacagtca
     1261 gggcagccgc cttcccgcgg agttcgacat cacagactac gttcgcgccg gtgagaatac
     1321 gctcgccgtc cgcgtgtacc ggtggagtga cggaagttgg ctcgaagatc aggactactg
     1381 gtggctttcc ggcatctatc gcgacgtggt cctctacagc ctacccgctg tcaacatccg
     1441 cgatttcacc gtgcgcacac cgctcgattc ggcttaccat gacgcgcgtc tccaggtgac
     1501 tgcgctgatc cggaacgaca gcgatgagcc gcaaaacgtc gaactcggcg tgacgcttct
     1561 cgaccgcgac ggccacgaag tctcactcga tcatgcggaa gatgcgaccg gtctgcgccc
     1621 cggacaggag agcgatatcg ccttcgacct gcccgccgag aatccggcga aatggtcgga
     1681 cgagacgctc tatctttaca c
//
LOCUS       DSCC01000393            3203 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1020474, whole genome shotgun sequence.
ACCESSION   DSCC01000393 DSCC01000000
VERSION     DSCC01000393.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1020474"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..2519)
                     /locus_tag="ENN56_04895"
     CDS             complement(<1..2519)
                     /locus_tag="ENN56_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74862.1"
                     /translation="MKDLTPAALERLDERFGAPVVLVTISARNASGELVTRRYASRGA
                     DDGSGGYRIGGGGDEYEPAIVSVGPITREHPSFAFGEIVLANPTTGGDLFSEGLGVRE
                     LDGARCEIAVIFADGDETPDDSIPLASGVVEQSRITVRGIELQWREYVLGEPDNFPDG
                     PRMTAMDYPHAPVANRNRLLPVVFGDLRGRAFDMTTPGNGTFPHVPVSTINAARQHLA
                     AYDATLGGERELLLRIGDYVIEVPDALLSAGGDYVQVDGTTFHLWMPPFRVNSATDST
                     VIDPEYASNANFDRYAHVPALTTLALDLPGASGVLGLFGAGSSPSAADVVIYTVYTKT
                     DAGDVYPGRVSVLMDDTGISGHTNIALGGSGLAVDAQEIGDHLSDWSDLQRLAVHVDG
                     VASTAGVDIRRVLMRVRFQCANVSAAFERQRFFRSMAGFTESVTDPLDDYRDGTYLHG
                     DRLSEPVEHPADLAEAILRNRAWGFDLTASSLVDTGADLVDPLDATVTECEVTSAASF
                     SVGDFALIEDEVVRVTDIDGTTLSILRGALGSRIACHPDGARVYVGGSDGDVDSMTFR
                     AARSALVSLDAAELVANGCMEGAYASGVADDWSVYDPDGIGALFADAGYVGHRAQGVF
                     RTSVGGGDPGVTQALNGLIPGERYRVECFAKAVSGASAVRVDTVQSMPFSSVFPIGAE
                     WARIEFDVTATDVAMTLRIRNAGGIGSIVVDAASVRLIRPWRMAFSLHDTTNGQRWLE
                     QSFLPQSGMRLGVSSDGKIAAILAHYESTSVVTFDETRILIEHESSGVTGASEVYIPA
                     IRARRGAPDDVASDVAVRFAPNRVTGGYDGFTGISVNTSDTG"
     gene            complement(2516..2956)
                     /locus_tag="ENN56_04900"
     CDS             complement(2516..2956)
                     /locus_tag="ENN56_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74863.1"
                     /translation="MAAWQWSYPFDSTDLATVHFDIAPANALCHWRAPHADSPQPRYT
                     LVETVDGGVRSYRVGDAGRRLVLRFVGLPVGDDTSDAALWGYNGVLRFLEAFCEFSGK
                     PFGIYDHTGTSAELRVRYVGGIETFRRTRDQYDGEIVLRQEAVS"
     gene            3062..>3203
                     /locus_tag="ENN56_04905"
     CDS             3062..>3203
                     /locus_tag="ENN56_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74864.1"
                     /translation="MSNPLSNAWHWVRQWFSTREKLPDLSRNDPCWCGSGKKYKHCHM
                     AED"
BASE COUNT          627 a         1036 c          957 g          583 t
ORIGIN      
        1 cccgtgtccg acgtgttgac agagatgccg gtgaagccat cgtagccacc ggtcacgcga
       61 ttcggtgcga accgcacggc aacatcactg gccacgtcgt ccggcgctcc tcgccgcgcg
      121 cgaattgccg ggatgtacac ctcagacgca ccagtaacac ccgatgattc gtgctcgatg
      181 agtatccgag tctcgtcaaa cgtcacgacg gacgtcgatt cgtagtgcgc gagaatggcg
      241 gcgattttcc cgtcggaaga cacgccaagt cgcatgccgc tttgcggaag aaacgactgc
      301 tcgagccatc gctgtccgtt ggtggtgtcg tgaagcgaaa acgccatgcg ccacggtctg
      361 atcagccgta cgctcgccgc gtcgacaacg atagagccga tgccgcccgc attacggatg
      421 cgcagcgtca tcgctacgtc ggttgcggta acgtcaaact cgatgcgcgc ccactccgcg
      481 ccgatgggaa acacagaact gaatggcatc gactgcaccg tatcaacccg cactgcagac
      541 gcgccgctca ccgccttcgc gaagcattcg acgcgatacc gctcgccggg tatgagcccg
      601 ttcagcgcct gcgtaacgcc cggatcgccg ccgccgaccg acgtgcggaa gactccttgt
      661 gcgcgatgac cgacgtatcc cgcgtccgcg aacagtgctc cgataccgtc cgggtcgtac
      721 acggaccagt cgtctgcgac gccgcttgcg tacgcgcctt ccatgcaccc atttgccacc
      781 aactcagcgg catccagtga tacgagcgcg ctccgtgctg cgcgaaatgt catgctgtca
      841 acgtcgccgt cggaaccgcc aacatatacg cgcgcaccgt ccggatggca cgcgatccgc
      901 gaaccgagtg cgccgcgcaa gatactgagt gtcgtgccat cgatatcggt cacgcgaacg
      961 acttcgtcct cgatcaacgc gaaatctccg acgctgaacg acgcggcact cgtcacttcg
     1021 cattctgtca cagtagcatc gagcgggtcg acgagatcgg ctcccgtatc gacgagactc
     1081 gacgcggtca gatcgaagcc ccacgcgcgg tttcgcaaga tcgcctcggc gagatctgca
     1141 ggatgctcca ccggttcgct gagccggtcg ccgtggaggt acgtgccgtc gcggtagtcg
     1201 tcgagtgggt cggtgacgct ttcggtgaag ccggccatcg agcggaagaa ccgttgtcgt
     1261 tcgaacgccg cgcttacgtt cgcacactgg aaccgcaccc gcatgagcac gcgacggata
     1321 tccaccccgg cggtgctcgc gacgccatca acgtggacgg cgagccgctg gagatcggac
     1381 caatcgctca agtggtcgcc gatctcctgc gcatccaccg ccaaaccgct cccgccaagc
     1441 gcgatattcg tgtgcccgga gatgccggta tcgtccatga gcacgcttac acgcccggga
     1501 tagacgtcgc ccgcgtcggt tttcgtgtag accgtgtaga tgacgacatc cgctgcgctt
     1561 ggcgacgatc ccgcaccgaa gagaccgagc acgcccgatg cgccgggaag gtcgagcgca
     1621 agggtagtaa gcgcgggtac atgcgcgtat cggtcgaagt tggcattcga cgcgtattcc
     1681 ggatcgatca ccgtcgaatc ggtggcgcta ttgacgcgaa acggcggcat ccataggtga
     1741 aacgttgttc catcgacctg tacatagtca ccgccggccg atagcagcgc atcaggcacc
     1801 tcgatgacgt aatcaccgat gcgcaggagc agctcccgtt cgcctcccaa agtcgcatcg
     1861 taggccgcaa ggtgctgccg tgccgcgttg attgtggaca ccggcacgtg tgggaatgtg
     1921 ccgtttcccg gcgttgtcat gtcgaacgcc cgaccgcgta gatcaccgaa gacgacgggc
     1981 aggaggcggt ttcgattcgc caccggtgca tggggataat ccatagctgt catacgcggt
     2041 ccgtcgggga aattgtccgg ctcgccgagc acgtattcgc gccattgtag ctcgatgcca
     2101 cgaacggtga tgcgcgactg ctcgacgact cccgacgcga gggggatgct gtcgtctggc
     2161 gtctcgtcac catcggcgaa gatcacggcg atctcacagc gcgctccgtc cagttcacgg
     2221 acgccgagcc cctccgagaa cagatcgccg ccggtagtcg gattcgcgag cacgatctct
     2281 ccgaacgcaa acgaggggtg ctcgcgcgta atcggtccca ctgagacgat cgccggctcg
     2341 tattcgtcac cgccgccgcc gattcgatat ccaccggagc cgtcatcagc gccgcgcgag
     2401 gcgtaccgcc gcgtaacgag ctcaccggat gcgttccgcg cggagatcgt caccaacaca
     2461 accggtgcgc caaagcgttc gtcgagccgt tcgagtgccg cgggtgtcag gtctttcacg
     2521 aaacggcctc ctgtctcaac acgatttcgc cgtcgtactg gtcgcgtgtc cgtcggaatg
     2581 tctcgatgcc gccaacgtac cggacacgca gctcggccga cgtgccggtg tggtcgtaga
     2641 ttccgaacgg ctttccgctg aactcgcaaa acgcttcgag aaaccgcagg acgccgttgt
     2701 atccccatag cgcggcatcc gacgtgtcat cgcccaccgg caggccgacg aagcgcagaa
     2761 cgaggcgacg tccggcgtcg ccaacacggt acgatcggac accaccatcg acggtctcga
     2821 cgagggtata tcgtggctga ggcgagtccg catggggtgc gcgccaatga cacagcgcgt
     2881 tcgccggtgc gatatcgaaa tgcacggttg ccagatcggt actgtcgaac ggataactcc
     2941 actgccatgc ggccatatcg tgtcacctcc aatcgccggt catggcgatt tgcacggcca
     3001 aaccgtacct tttcttgtta cctcgtatct gtctcgaatt tccgtgaagg acggtattca
     3061 tatgtcgaat cctctcagta acgcgtggca ctgggtgcgt cagtggttct caacacggga
     3121 gaagcttccc gatctcagtc ggaacgatcc ctgctggtgt ggaagtggca agaagtacaa
     3181 gcactgccat atggccgagg atc
//
LOCUS       DSCC01000394            1688 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048489, whole genome shotgun sequence.
ACCESSION   DSCC01000394 DSCC01000000
VERSION     DSCC01000394.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048489"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..>1688
                     /locus_tag="ENN56_04910"
     CDS             <1..>1688
                     /locus_tag="ENN56_04910"
                     /inference="COORDINATES: protein motif:HMM:PF13245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase PcrA"
                     /protein_id="HDS74865.1"
                     /translation="QGIWMGTFHSICARILRHDGERVGLDRNYSIYDVEDTKTLIRRI
                     FRRRNIDEKMLKPTRFREIVSTAKNQLRTPEEYARENAGPLVPLIAEMFAEYQSELRA
                     NNAVDFDDLLLMPVELFRSYPDVLASWQSRFDHLLIDEYQDTNHAQYLLIRHLADQHH
                     NLCAVGDDDQSIYGWRGADISNILSFEKDYPEAAVIRLEQNYRSSGTILKAASAVVRN
                     NTARKGKELWTDRDAGESIIAAALEDEQAEAGWIRDRIVSLRAQGHRYSDMVVLYRTN
                     AQSRSIEETFVRSRTAIPYTVVGGLRFYERKEVKDILAYLRVIDNPRDMESLRRIINV
                     PKRGIGERTVEKLVETSAETNLTLLEVIRDKDLVPVSNSSQQGQAIAKLAVFIDELIG
                     AKETMPIHEMMNRVMAETGYIAALRDEKSVESETREQNLQELLAGATSFADAAQAEGR
                     DTTVSAFLQEVSLVTDIDQWEDSSEAVTLMTLHSAKGLEFPIVFVAGLEETLFPLGGM
                     QRSEEELEEERRLFYVGLTRAEERVFLSLAQRRRRYNEYLAMAPSRFLNELPPE"
BASE COUNT          416 a          457 c          461 g          354 t
ORIGIN      
        1 acagggtata tggatgggaa cattccattc gatctgtgcc cgcatcttgc ggcatgacgg
       61 cgaacgagtc ggcctcgacc gcaactattc catctacgac gtcgaggaca cgaagacact
      121 cattcgccgc attttccggc gccgcaatat cgacgaaaag atgctcaaac caacgcggtt
      181 tcgagagatt gtctcaacag cgaagaatca gcttcgtaca ccagaggaat acgcacggga
      241 gaatgccggt ccactcgtgc cgctaatcgc cgaaatgttc gccgaatacc aatcggaact
      301 tcgcgcgaac aacgcggtcg atttcgatga tctgcttctg atgccggtcg agttgtttcg
      361 ttcgtacccc gatgtgctcg catcctggca aagccgtttt gaccatttac tcatcgatga
      421 ataccaggac acgaaccacg cccagtacct gctcatacgt catctcgccg atcaacatca
      481 caacctctgc gcggtcggtg acgacgatca gtcgatctat ggctggcgag gcgccgatat
      541 ctcgaatatc ctcagtttcg aaaaggacta tcccgaagcg gctgtgatcc ggctcgagca
      601 gaactatcgt tcgtcgggaa caatcttgaa agcagcgagt gccgtcgttc gcaataacac
      661 tgcacggaag ggtaaagagc tgtggaccga tcgagacgcc ggtgaaagca tcatcgcagc
      721 cgctcttgaa gacgagcaag cggaagcggg gtggattcgc gaccgaattg tatcactccg
      781 cgcacaaggt catcgatata gcgacatggt cgttctctac cgcacgaatg cccaatcgag
      841 atcgattgaa gagacattcg tccggtctcg tacagcaatc ccatacacgg ttgttggcgg
      901 actcaggttt tatgaacgca aagaagtaaa ggacatcctc gcgtatctcc gcgttatcga
      961 taatccacga gacatggaga gcctgcgacg cattattaac gtgccgaaac gcggaatcgg
     1021 tgagcgaacc gtcgaaaaac tcgtcgaaac gtccgcggaa acgaatctta cattgctcga
     1081 ggttattcga gataaggatc ttgtccccgt ttcgaattcg tcacagcaag ggcaggcgat
     1141 cgccaaactc gcagtgttta tcgacgaatt gatcggcgcg aaggaaacga tgcccatcca
     1201 cgaaatgatg aaccgggtca tggcggagac cggttatatc gcagcgcttc gcgacgaaaa
     1261 gagcgttgaa tcggaaactc gcgagcaaaa cctccaagag ttactcgcgg gcgccacgag
     1321 tttcgccgac gcagcgcaag ctgaaggtcg cgatacgacg gtaagcgcgt tcctgcagga
     1381 ggtctcgctc gttaccgaca tcgatcaatg ggaggattcg tccgaagccg ttacgctcat
     1441 gacgcttcac agtgcaaagg ggcttgagtt tcccatcgtt ttcgttgccg gattggagga
     1501 aacgctgttc cctctgggag gtatgcagag gtcggaagag gaacttgagg aagagcggcg
     1561 gttgttttac gtcggtctta ctcgggccga agaacgggtt ttcctttcgc ttgcacagcg
     1621 ccgtcggcga tacaacgagt acctcgccat ggcgccgtcg cgtttcctca acgagttgcc
     1681 acctgaac
//
LOCUS       DSCC01000395            1804 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1044312, whole genome shotgun sequence.
ACCESSION   DSCC01000395 DSCC01000000
VERSION     DSCC01000395.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1044312"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            132..1667
                     /locus_tag="ENN56_04915"
     CDS             132..1667
                     /locus_tag="ENN56_04915"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01743.18,HMM:PF12627.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDS74866.1"
                     /translation="MKRGENSKRSIRRRKHRQSGYPGGMPNGSRPSFPALMWTRTSPY
                     WVSRDSGTHLTDSRERTSVETQGIVTVRDAAKRSPDFAPARRVVQRLRDAGHTAFLVG
                     GGVRDLVLGRAPKDFDIVTSARPDEITQLFRRTVSVGAQFGVITVLDEGGPHEVATFR
                     AEGGYEDKRRPGWVEYADLHADLQRRDFTINGLVLDPDTFEVIDHVGGLDDLERGVIR
                     TIGDPGARFGEDALRMIRAIRFAARFGFRLDRNTRRALIEHHGDIKHVATERIWQELE
                     RMLAHRNRAKALVLMRDTCVLDEVLLELAALAKTSRGAWSPWHRTVRRITALPDDAPL
                     SAVFAALLADVVELDDPPRAEWDADGPISPRGAKEAAKILARLRAPNTLQKAVAAVIE
                     RRWLWRHCSVLRLGSVARILRTQHAESLLAFWQADAASMNRLTAFAPIHHVAHRLQLR
                     GRMYGGPARPPVTGHDLRARGLEPSSRFAELLAEAERRWLEERLETTNAVTLWLDGQM
                     LRR"
BASE COUNT          369 a          588 c          529 g          318 t
ORIGIN      
        1 cggcgtagga acagaccaag tcggagtcat cgtacccgag cgtccgtctc gcgtcatcgg
       61 aaccagcaca cccgaatgcg tcgaaatcgg gctgtatcac ggatggctcg gcggcacact
      121 ccgcctgatc gatgaaacgc ggcgagaact caaagagatc gatccgacgg cggaaacacc
      181 ggcaatctgg atatccgggg ggcatgccga atggatcgcg cccgagcttc ccggcgctca
      241 tgtggacgcg gacctctccg tattgggtct cgcgcgactc tggaacgcac ttaactgatt
      301 cacgagaacg aacatccgtg gaaacgcagg gaatcgtaac cgtacgcgac gccgcgaaac
      361 gatcgccgga tttcgcaccg gcccgccgag tcgtccaacg gctccgtgac gccggacaca
      421 ccgcgtttct cgtcggaggt ggcgttcgcg atctcgtatt ggggcgcgca ccgaaagatt
      481 tcgacatcgt cacatcggcg cgaccagacg agatcacaca gctctttcgc cgaaccgtct
      541 ccgtcggcgc gcagttcggt gtgatcaccg ttctcgacga aggcggcccg cacgaagtcg
      601 caacgttccg cgccgaaggc gggtacgaag acaagcgccg tcccggctgg gtcgaatacg
      661 ccgacttaca tgcggacctc caacgccgcg atttcaccat aaacggactc gtgctcgacc
      721 cggatacctt tgaagtcatc gaccacgtag gcggcctcga cgacctcgaa cgcggcgtta
      781 tccgcactat cggtgacccc ggcgcacgct tcggcgaaga cgcactgcga atgatccgcg
      841 cgatccgatt cgccgcccgg ttcggcttcc ggctcgaccg gaacacccga cgcgcgctta
      901 ttgagcacca tggcgacatc aagcacgtag caacggagcg gatatggcag gaacttgaac
      961 ggatgctcgc tcaccggaac cgcgccaagg ctctcgtact gatgcgcgat acgtgtgttc
     1021 tcgacgaggt gctcctcgag cttgccgctc tcgcaaagac ctcgcgcggc gcatggtccc
     1081 cgtggcaccg caccgtccgc cgaatcacgg cactacccga tgatgcgccg ctctccgccg
     1141 tgttcgccgc actacttgca gacgttgttg agctcgacga tccaccgcga gccgaatggg
     1201 acgctgacgg gccgatatca ccacgcggtg caaaagaagc ggcgaaaatc ctcgcgcgac
     1261 tacgtgcacc gaacacgctg caaaaagccg tcgccgcggt tatcgagcga cgctggctgt
     1321 ggcgacactg ctcggtacta cggctcggaa gcgtcgcgcg tatcctccgc acgcaacatg
     1381 ccgaatcact cctcgcgttc tggcaagcgg acgcggcaag catgaaccgc ctcacggcat
     1441 tcgcgccgat ccaccatgtc gcgcatcgac tccaactccg aggccgaatg tatggcggtc
     1501 cggcacgtcc gccggtgacc ggtcacgacc tccgtgcgcg aggactcgag ccgagttcgc
     1561 gtttcgccga gctgctcgca gaggccgaac ggcgttggct cgaagagcga ctcgaaacga
     1621 ccaacgcggt tacactctgg ctcgacggac aaatgctgcg tcggtaatcg attacgatat
     1681 tgcggaactc accgaaatcg ggcatatttg tttcaagcat tgttatttct accagtaacc
     1741 ttgttcgttg tcgcgtacac aggtttcctc ttttttccgt gggggggcag ggaagccatg
     1801 agac
//
LOCUS       DSCC01000396            2094 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1036202, whole genome shotgun sequence.
ACCESSION   DSCC01000396 DSCC01000000
VERSION     DSCC01000396.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1036202"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            1146..>2094
                     /locus_tag="ENN56_04920"
     CDS             1146..>2094
                     /locus_tag="ENN56_04920"
                     /inference="COORDINATES: protein motif:HMM:PF00664.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS74867.1"
                     /translation="MLRHPLPPQILEWFSQQNVKDGDILLSIPNDITFEGEYAERWVV
                     VTHDAVMMLKEKDGRISAARNFAMADIEAARVDPRVGSGFLQVKVNGNWAEVARFSNA
                     RATWFERIAMKITRFKETGELEITPEDEKEENRCPTCGLLLRSSTDVCPRCINKHRVF
                     GRLLILMKPYWPWAAGTLALVFLGLGLDLIPPQLSRILMDDVFVRDPNRAFLMPAWAR
                     EYIGTSPIAMLFWVVFVGMAGVQLLRILINIVIARLSVTVGTKITHDLRHRLFARLSE
                     LSVKYYDRHQVGILMTRVTSDTEALHGFIIQSTQGFLVNI"
BASE COUNT          505 a          550 c          579 g          460 t
ORIGIN      
        1 gccgtgacat gcctgcgcgg ggacgaggta tggcgagcag ccgataaagg gctgtggtca
       61 gaagatctgg ccgtagcggc actgaagacg gtattcacca agaaagacga accgccgaga
      121 accggctgcg agaatccgga agcgatcatc atcgagtacc gcgacggatt tcgcgcaaca
      181 gcgctacagc tgaatggata cattgtcgat tgggcagtcg cgatgcgtat aggcgggaaa
      241 gtcgtagcga cggaattcgc cgtcgaatac gcagcaccgt tctcgcactt cggatatctt
      301 agtcgcaaca tcgaaaagat gtttcttacc ggaaagccgg gctatccgtg ggaacgaacg
      361 tttgtcacaa ccggcgtcat aaatactgcg atggtctcgg tcgcgacggg tggtaaacgc
      421 atcgaaacgc catggcatga agcgaattac acctcgtaca gggaaatgcc gattcgttcg
      481 acatcgggaa gcgaccggga aagaccggtg aaggatataa accgatcgga tactcggaaa
      541 acgaatagcg gcgtttcgaa caaaggaaac gggtgaacga cgcgaagtcg ttcacccgtc
      601 cgtaagatcg gtgcctgttt ggatttgagg gccgcagtca ggtaacgacg cgtcaaccct
      661 cgcaggtcgg tccgccgtta ggcgctacac ccccaacagt cacctgcaca acaataagaa
      721 tccaaatgac cacctccttt ccgtgctgcg aatatagtac accgcttcgt tacgcttgtc
      781 aacaccaatc ccccaatacg agaagacccg tagaacgagt gcgcacgaag cgtgagaacg
      841 acgtcgatac gataggtacg tttttttcaa aaatggtggt tcatagtgtt gacaataccg
      901 tgtactgtat gcatcttctt tcgcaatccc cattcaagct gacagatttt ggcgtcggct
      961 tgaatagcga tacgttgccg aaacaggagt ggcagcacct caagttcacc tcataacgcg
     1021 agctttccta tagcgttccg ctaatcgcag attgattcgt cgcgctcgtg tcgagcgtgg
     1081 tacccgatac ccatgcgcaa gcggtatgtc ttaatccgac gtccacaagt cctggagata
     1141 tttcaatgct acgccatccg cttcctccgc agattttgga gtggttcagc cagcagaacg
     1201 taaaagacgg tgacattctc ctttcgatcc ctaacgacat cacgttcgaa ggcgagtatg
     1261 ccgaacgttg ggtcgttgtg acacatgacg ccgtcatgat gttgaaggaa aaggacggac
     1321 gcataagcgc cgcccggaac ttcgccatgg cggatatcga agccgcacgc gtcgatccgc
     1381 gcgttggtag cgggttcctc caagtgaaag tgaacggcaa ctgggccgag gtcgcacggt
     1441 tctcaaacgc tcgagcaaca tggttcgagc gaatcgcgat gaagatcacg cgattcaaag
     1501 agactggcga actcgaaatc acgccggaag acgagaaaga ggagaatcgc tgtccaacct
     1561 gcggccttct cctccgcagt tcaacggatg tctgtccgcg ctgtatcaac aaacatcgtg
     1621 tgttcgggcg gctcctgata cttatgaagc catattggcc gtgggccgcc ggtacgttgg
     1681 cgttggtgtt tctcggcctc ggtctggacc tgattccgcc acagctctca cgaatcctca
     1741 tggacgacgt attcgtacgc gatccgaatc gcgcttttct catgcctgcg tgggcgcgcg
     1801 aatacatcgg tacgagtccg attgcgatgt tgttctgggt ggttttcgtc ggaatggcgg
     1861 gtgttcagct cttgcgcatt ctcatcaaca tcgtcattgc gcgactgtcg gttactgtgg
     1921 gtacgaaaat aacgcacgat ctgcgacaca gattgtttgc gcgtctttcc gagctgtcgg
     1981 tgaaatacta tgaccgtcat caggttggta ttctcatgac gcgtgtaaca agcgataccg
     2041 aggcgctaca cggtttcatc atacagtcga cgcagggatt cctggtgaat atcc
//
LOCUS       DSCC01000397            1688 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1048485, whole genome shotgun sequence.
ACCESSION   DSCC01000397 DSCC01000000
VERSION     DSCC01000397.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1048485"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(27..1127)
                     /locus_tag="ENN56_04925"
     CDS             complement(27..1127)
                     /locus_tag="ENN56_04925"
                     /inference="COORDINATES: protein motif:HMM:PF00557.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HDS74868.1"
                     /translation="MTRSDDMAIKRVRIREFMEIHGLGGVLFTLQKNFSWYTSGGRGY
                     IATTTDKSAGQILATPNQDYLIANNIETNRFLKEEGLGELGIRPLSYRWDADASDINQ
                     LTERVLAGKKHTTDVGPHAAEIDHLRYSLTPLEVERYRALGRDCEAAMNDVARSIEPG
                     MTEWQIAGRLQCALADKQVIGAVVLVAADERISLYRHPIPTNKRLENRALLVTCGRRH
                     GLIVSLSRMVSFGAVSDDLRRRHDACMAVDATFIESTTVGADVDEIVQKAIDTYEEHG
                     FTEEWTLHHQGGGTGYETRDFRGTLHSKEVVQPWQAYAWNPSITGTKTEDTIIATPDG
                     PEIISLSKDWPVKEFNTARGGPRIVRPDILVR"
BASE COUNT          300 a          530 c          493 g          365 t
ORIGIN      
        1 gccctctccg ctatcagtaa cctctcctac cgcaccaata tatccggtcg gacgatgcgc
       61 ggcccaccgc gagccgtgtt gaattccttc accggccaat ccttcgagag gctgatgatc
      121 tccggtccgt cgggcgtcgc gataatcgtg tcctcagtct tcgtgccggt gatcgacggg
      181 ttccacgcgt acgcctgcca cggctgcacg acttccttgc tgtggagcgt gccgcggaaa
      241 tcgcgcgtct cataccccgt tccgccgccc tgatggtgga gcgtccactc ctcggtgaac
      301 ccatgctcct cgtacgtatc gatcgccttc tggacgatct catcgacatc cgcaccaacg
      361 gtcgtcgact cgataaacgt cgcatcgacg gccatgcagg cgtcgtgacg gcggcgcaga
      421 tcgtcggaaa ccgccccgaa gctcaccatg cgcgagagcg agacaatcag cccgtgtcgc
      481 cgtccgcagg tcaccaggag cgcacggttc tcgaggcgct tgttcgtggg aatcggatgc
      541 cgatacaggc tgatgcgttc gtccgccgcg acgagtacga ccgcgccgat cacctgttta
      601 tcggcgagcg cgcactgaag ccgtcccgcg atctgccact cggtcattcc cggctcgatc
      661 gaccgcgcga cgtcgttcat cgcggcttcg cagtcccgcc cgagcgcgcg ataccgctca
      721 acttcgagcg gtgtgaggct gtagcgcagg tggtcgattt cggcggcgtg cggcccgaca
      781 tccgttgtgt gcttcttccc ggcgaggacg cgctcggtaa gctggttgat atcggacgcg
      841 tcggcatccc aacggtagga aagcggccgg ataccgagtt cgccgagtcc ctcctctttc
      901 agaaaccggt tcgtctcgat gttattcgcg atcaggtagt cctgattcgg cgtcgccagg
      961 atttggcccg cgcttttgtc ggtcgtcgtc gcgatatagc cgcgtccgcc gctggtatac
     1021 caggaaaagt tcttctgcaa cgtaaagagc acgccgccga gtccgtgtat ttccatgaat
     1081 tcgcggattc gtacgcgctt gattgccatg tcgtcggatc gagtcatcgg aggtgccttc
     1141 ctgtgtcgag tgcgggagta gagaagtagt aatatcgcag catccgacgg ttatgaccaa
     1201 cgagcgaacg cgagctgacg ctcgcgttcg ttggggcgaa gacacttgtt gtcttcgccc
     1261 agtgcggctt cataggaacc cataaaaacc gttgcttcgt tgcgaagtcg tgaaggtggt
     1321 gcggcggttt cgccttaacg aacccgtagc gtagcgacgg ttcgcggtgc gactaaagtc
     1381 gcgcgctaca attgcatagc ctgccttcgc aggctcaacc gtacgtgtta accgtaccga
     1441 tagtccgcgt aggcggactt ccttgcgaag ccgggttatt attgacagcc cgcgaattta
     1501 ttcgccgggc gaaccggcgc gtcgggctat cttctcgacg agttccaaaa cgcaccattc
     1561 gtttcgcggt gcgactgaag tcgctcgcta caatcgcaaa gcctgcctac gcaggctcaa
     1621 ccgttcgtgt taaccgtacc gccagtccgc gtaggcggac ttcgcaacta cagcccgcgg
     1681 atttatcc
//
LOCUS       DSCC01000398            3502 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018126, whole genome shotgun sequence.
ACCESSION   DSCC01000398 DSCC01000000
VERSION     DSCC01000398.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018126"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1143)
                     /gene="glnA"
                     /locus_tag="ENN56_04930"
     CDS             complement(<1..1143)
                     /gene="glnA"
                     /locus_tag="ENN56_04930"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDS74869.1"
                     /translation="MTPKDVIKMAQENGAEMLDLKFTDFPGMWQHFSVPIEFLDEDLF
                     EDGHGFDGSSIRGWQAINESDMLVIPDPDTALMDPFTAHPTISMICEIHDPITRERYS
                     RNPRNVAHKAEAYMQSTGLADTAYVGPEAEFFIFDDIRYDQTANSGYYYIDSIEGRWN
                     TGREENPNLGYKPGFKGGYFPVPPTDKLNDIRTEMVLQMIKSGLPVETQHHEVATAGQ
                     AEIDLRFSPLLRMSDMMMMYKYIVKNVAARHNKTVTFMPKPLFGDNGSGMHTHMSLWK
                     AGKPLFAGDRYAGLSEMALHFIGGLLKHAPAVLAISNPTTNSYKRLVPGYEAPVNLAY
                     SQRNRSASCRIPMYSPSPKAKRVEFRCPDPSCNPYLAFSAILMAGLD"
     gene            1447..>3502
                     /locus_tag="ENN56_04935"
     CDS             1447..>3502
                     /locus_tag="ENN56_04935"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01074.20,HMM:PF09261.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74870.1"
                     /translation="MWELSMMNNGCRWIDGRPIIALDSLGRNEHITEWLALGPYRFQW
                     PGRFTRDVNIGAADIVEDDPIGVERTVRPVLGMRHGNLDVPNGHSEWIQLSGATYYDL
                     KTRYPEQDTALAWAVTYLSATKPTDIVVQTREWIDRRRCLVETMLDGVTVNVGDAPFL
                     LRVDPGEHVLMVKIAGGASGNVAWHVDAVIGRPIEVADEIGIALVRSSGYWRGNEQAP
                     RLEIDLALVNHGDAEITLKSVFAVGSNGERGPQLANVTVEPRTIAPARLSAPLGDAKP
                     ASRCTFHIHVDDASVPVDIDVPPLPDPGIIHVVEGFHCDPVWVSDQHHYNLVSLENVR
                     QLLDGCLANPAYRCFLHEIDYLKPFVDEYPDYRAILFGLIQRGQVGLGSSYNQPNENN
                     CSGEGIIRNLLYGDGFHRRFLGGDPLVYHAWDVFGHIPQLSQILAKAGCIGVIWSKHI
                     WGFPPIFNHISPDGTALPHIRTPYGWGTHGIDRLRESVQPLVSEKHTYGLRRHLVMDS
                     SDFHSPSSWMIGRTEEMADSFPTIVMSTPEAYLQGHIDDGAAFPITSRNPCQYHIGTQ
                     HSRSELKIANRLAENTLYSAEMWSTFASLMGADYPDLAIDKAWRQILFGQHHDALTGT
                     PCDVSYLDLMNGYREALELARSVNDSARHYIASHVKLFEKGNTYIVFNPLNWSRQGSV
                     RLP"
BASE COUNT          798 a          980 c          926 g          798 t
ORIGIN      
        1 atcgagtccc gccataagaa tcgccgagaa ggcgagatac ggattacatg acgggtccgg
       61 acagcggaat tccactcgct ttgccttcgg agacggtgaa tacatcggga tacgacacga
      121 tgccgaccgg ttccgttgcg agtatgcaag attgaccggc gcttcgtacc cgggcactaa
      181 ccgcttatac gagttcgtcg tggggttgga tattgcgagt actgcgggcg cgtgttttag
      241 aagaccgccg atgaagtgca gcgccatctc gctaagccca gcgtaacgat caccggcaaa
      301 caacggtttt ccggccttcc acaaactcat gtgcgtatgc atgccgcttc cgttatcacc
      361 gaatagcggc ttcggcatga aggtcaccgt tttgttgtga cgggcggcga cattcttcac
      421 gatgtacttg tacatcatca tcatgtcgct catccgaagc agcggagaga agcgcagatc
      481 aatttcggcc tgtcccgccg ttgcgacttc gtggtgttgg gtctctacgg gaaggcccga
      541 tttgatcatc tgaagcacca tctcggtgcg gatatcgttg agcttatcgg ttggtggaac
      601 cgggaaatac ccgcccttga aaccgggctt atatccgagg ttcggatttt cctcacgccc
      661 cgtgttccag cgaccttcaa tcgagtcgat gtagtagtac ccggagtttg ccgtttggtc
      721 gtagcgaatg tcatcaaaga tgaaaaactc tgcttcaggc cccacataag ccgtatcggc
      781 gagaccagtc gactgcatat acgcttcggc cttgtgcgca acgttgcgcg gatttcgcga
      841 ataccgctca cgggttatcg gatcgtgaat ttcgcaaatc atactgatcg tcggatgagc
      901 cgtaaacgga tccatcaacg ccgtgtccgg atcgggaatc acgagcatat cgctctcgtt
      961 aatcgcctgc cagccacgaa tcgatgaacc atcgaatcca tgtccatctt cgaacagatc
     1021 ttcatcgaga aactcaatcg ggacggaaaa atgctgccac attcccggga aatcggtgaa
     1081 tttcagatcg agcatctcgg cgccgttctc ctgcgccatc ttgatgacgt cttttggcgt
     1141 cattgccatg gtacgtcgct ccctttcctt gcgaattggc cgattgtcgt gctcgatgca
     1201 acctcacgga ccttccgtta cgtcacatcg gcaacgacca cttctacgcc gccttggcct
     1261 cgttgcgaat aagtataggg agaccatgtt tccgcgcgtt tgcgctgatg ttaattcgat
     1321 gtaaacaacg tgcaacgacc gacggctaac cgtttccggg acacacgtgc cttgccccgc
     1381 gaaccccgga atgcgtccat catagggtga aaaaacactt caagaaacgg tagatatacc
     1441 gacttcatgt gggagttatc gatgatgaac aatgggtgtc gctggataga cggacgaccg
     1501 attatcgccc ttgattcgct tggtagaaac gagcatataa ccgaatggct cgccctcggt
     1561 ccgtatcgtt ttcaatggcc gggaagattc acgcgagacg tgaatattgg cgccgccgat
     1621 atcgtcgagg acgaccctat cggtgtagaa aggaccgtac gtcctgttct cggaatgcgc
     1681 cacggtaatc tcgatgtgcc aaacggtcat tccgagtgga tccagctttc cggagcgacg
     1741 tattacgacc tcaagactcg ctatccggag caagacaccg cactcgcttg ggcggtgaca
     1801 tatctttcag cgacgaaacc caccgatatc gtcgttcaaa cccgtgaatg gatcgaccga
     1861 cggcgttgcc tcgttgaaac aatgcttgac ggcgttacgg tcaatgtcgg cgatgcgccg
     1921 tttctccttc gtgtagaccc gggcgagcat gtgctaatgg tgaaaatcgc gggtggcgcg
     1981 agtggaaatg ttgcatggca cgtcgacgcg gtaatcggac gaccaatcga agttgcagat
     2041 gagattggta tcgcactcgt gcgttcaagc ggatactggc gcggaaacga gcaggcgcca
     2101 cgccttgaaa tcgatctcgc actggtaaat catggtgacg cggaaatcac tctcaagagt
     2161 gtttttgccg ttggatcaaa cggtgaacgc gggccgcaac tcgcgaatgt aaccgttgaa
     2221 ccacggacaa tcgctccagc tcgtctatcg gctccactgg gcgatgctaa accggcgtct
     2281 cgatgcacgt tccacataca cgtcgacgat gcatccgttc ccgttgatat cgatgtccca
     2341 ccgctgcctg atccgggcat cattcacgtc gtcgaaggtt ttcactgcga tccggtctgg
     2401 gtaagcgatc aacatcacta caatctcgtt tccctcgaga acgtccgaca gctgctcgac
     2461 ggatgcctcg caaatccggc gtaccggtgc tttctccatg aaatcgacta ccttaagcca
     2521 tttgtcgacg agtatcccga ttaccgagcg atactattcg ggctgattca acgcggacaa
     2581 gtcgggcttg gatcgagtta taaccagccg aacgagaaca actgctccgg tgaaggcatc
     2641 attcggaatc tgctttacgg tgacggtttt caccgacgtt tccttggagg cgatccgctt
     2701 gtttaccacg cgtgggacgt gttcggacat attccgcagc tgagtcaaat cctcgcaaaa
     2761 gctggttgca tcggagtaat ctggtcgaaa catatttggg gattcccacc cattttcaac
     2821 cacatttctc ccgacggaac ggctctccca cacatccgaa cgccatacgg atggggtaca
     2881 cacggcatcg accggcttcg cgaatcggta cagccgctcg tctccgaaaa acacacgtac
     2941 ggattacgcc gtcatttggt gatggattcg tcggacttcc attcgccgag ctcgtggatg
     3001 atcggccgca ccgaagagat ggcggattca ttcccgacga tcgtcatgtc gacgcccgaa
     3061 gcgtatctac agggccacat tgacgacggt gcagcgttcc caatcacgag ccgcaaccct
     3121 tgccagtacc acatcggcac gcagcactcc cgttccgagt tgaaaatcgc caatcgactt
     3181 gctgagaaca cgctctactc ggccgaaatg tggtcgacct tcgcctcgct tatgggtgca
     3241 gattacccgg acctcgcgat cgacaaagcg tggcgccaga tactcttcgg tcagcatcat
     3301 gacgcgctga cgggaacgcc gtgcgatgtg tcgtatctcg acttgatgaa cggatatcgc
     3361 gaagcgctcg aactcgcaag atcagttaac gacagcgcga gacactacat agcgagtcat
     3421 gtaaagcttt tcgagaaggg caacacgtac atcgttttca acccgctgaa ctggtcgaga
     3481 caaggttcag tacgacttcc ga
//
LOCUS       DSCC01000399            1519 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1055848, whole genome shotgun sequence.
ACCESSION   DSCC01000399 DSCC01000000
VERSION     DSCC01000399.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1055848"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(182..787)
                     /locus_tag="ENN56_04940"
     CDS             complement(182..787)
                     /locus_tag="ENN56_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDS74871.1"
                     /translation="MAYELPKLPYAFNALEPHIDARTMEIHHGKHHAAYIANVNAALE
                     GTEFANKPVEEVLRNISAVPEKIRQAVINNGGGHANHSLFWQVMGPNKGGQPTGAVAD
                     AINGAFGGFDTFKTLFSDAAAKRFGSGWSWLVVKNGKLEVYSTANQDSPIMQGGTPIL
                     GLDVWEHAYYLNYQNRRPDYVSAWWNVVNWDKVAENLKNAK"
     gene            1048..>1519
                     /locus_tag="ENN56_04945"
     CDS             1048..>1519
                     /locus_tag="ENN56_04945"
                     /inference="COORDINATES: protein motif:HMM:PF00370.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDS74872.1"
                     /translation="MASVRRMVAVDLGASSGRAILGTLGDTLRLESVHRFPNGPVSVE
                     GILRWDFERLWDGVREGIRRAGTLSDGPIESVGVDTWGVDYGLIGKDGELLEAPIHYR
                     DDRTDGAMDAVFQRVPRREVFEATGIQFIQLNTLYQLFAHARTDRSALDRARYLL"
BASE COUNT          302 a          429 c          426 g          362 t
ORIGIN      
        1 cgtcagcagg cgctcggacg agtgattgga gaaaacgtcg taccgcaacc gcgcgaagag
       61 tagttggtat tcaacaaaac gatcaacgcg ctcggtagcg tagagaaagc aaaaaaagcg
      121 ggtcagattt tcattccgac ccgcttcgcg ttataggtgt gttggacaga tcacaccgag
      181 gttacttcgc gttcttaagg ttctccgcca ccttgtccca attcacgacg ttccaccacg
      241 ccgagacgta gtcgggccga cggttctggt aattcaagta gtacgcgtgc tcccagacat
      301 cgaggccgag aatcggcgtg ccgccctgca tgattgggct gtcctgattc gccgtgctgt
      361 aaacctcgag cttgccgttc ttcaccacga gccacgacca accactgccg aatcgcttcg
      421 cggccgcgtc actgaagagc gtcttgaatg tatcgaatcc accgaacgcg ccgttgatcg
      481 cgtcagcaac cgctccggta ggctgaccgc ccttgttcgg ccccataact tgccagaaca
      541 gtgaatgatt cgcatgtccg ccgccgttgt tgatgacggc ttgccgaatc ttttcaggta
      601 ccgcggaaat gttcctgagc acttcctcga ccggcttgtt tgcgaattcg gtgccttcaa
      661 gcgcggcgtt tacattcgcg atgtatgcgg cgtgatgctt accgtgatgg atctccatgg
      721 tgcgcgcgtc gatatgcggt tccagcgcgt tgaatgcgta cgggagtttc ggaagttcgt
      781 aggccatgct gtatcccctt cgtgtatttc agtgagtaat ctttcacgat cgaccggtcg
      841 caattccgtt ggactacttc gctattcccg accattattg taaatgagtc tcatgtaata
      901 tagtcaatcc gaacccaatt gtccagtgct gatcctgttt gctccacgaa accgtgtccg
      961 gtacattccg tatcacccgg tcaacttgcc gcagtatcgt gatttgggcc ggttgtacgc
     1021 atccacgaac atgcgaggag aacactcatg gcatccgtac gacgtatggt cgcggtcgat
     1081 ctcggcgcgt cgagtggtcg cgccattctt ggaacactcg gcgatacgct gcgcctcgaa
     1141 tcggttcacc gttttccgaa cggaccggta agcgtcgaag ggatcctccg atgggatttc
     1201 gaacgactgt gggatggtgt tcgcgagggc attcggcgtg ctggaaccct ttcagacggg
     1261 ccgatcgaga gcgtcggggt ggatacgtgg ggcgtcgatt atgggctcat cgggaaggac
     1321 ggcgagctac tcgaagcgcc gattcattac cgcgacgatc gcaccgatgg ggctatggat
     1381 gcggtattcc agcgcgttcc gcgtcgtgag gtgtttgagg cgaccggtat ccagtttatt
     1441 caactcaata cgctctatca gctcttcgca cacgcgcgca ccgaccgctc cgctctcgat
     1501 cgagcgcgct atctcctga
//
LOCUS       DSCC01000400            3684 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1016918, whole genome shotgun sequence.
ACCESSION   DSCC01000400 DSCC01000000
VERSION     DSCC01000400.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1016918"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..2950)
                     /locus_tag="ENN56_04950"
     CDS             complement(<1..2950)
                     /locus_tag="ENN56_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HDS74873.1"
                     /translation="MMRPSHDIRRYSRGTLIMRYIGMLCAMLAIGAVFVACSEKTSDP
                     IRRGPHPAGFVDATSENFHGARVRAADFGGNASCLECHTVVAADSTAEAIAISCANSP
                     GCHARSETATSLSCGTCHERSELPPSHQEHFAAGAGGCALCHAATFSTTGALLDSVHM
                     NAVLNVALSVTVEGTFNADDMTCSDSRCHGITRPNTSPAWGATGNLTCQSCHAIADLS
                     DKHDTHFAQSAANCGICHASTAVSATELQPDTETHANLTADVVFATSHGGSFDATAGA
                     CSDTYCHGVGVTTPAWTGTTDLDCQSCHAFAELPDAHAKHLAKNDDCSLCHAITAASN
                     AAIANPAFHANLQTDVVLSTTINGEFVSATQTCTNTSCHGVQSPAWTAVVDLDCNSCH
                     AKADLPGAHQAHFARSPANCETCHASTATDSSAIKDPSLHMNFTIDVALIDGIDGTYD
                     STTETCSNTYCHLDATPTPAWSGSTVFRCQSCHSYASLADPHPPHVSFVSNDCSVCHS
                     ETVSGPYTMINPSLHADQIVQVAMNAQAGVGAVYDDVTNSCSNTYCHVSGAPTWSAGL
                     SLDCNSCHAKAELPGAHQAHFARSPANCETCHAKTATDSSAIKDPLLHMNYAVDVAMI
                     DGIDGTYDSATGTCSDTYCHLDATPTPAWNGTTDLRCQSCHSYASLPEPHPVHVDLVS
                     NDCSVCHSETVSGPYTMINPSLHTDQIVQVAMNDVVEGTYDTQTNSCADTYCHRSGTP
                     VWTTSTLDCNSCHAVAELPVSHPAHFKSMEQNCETCHASTAQDSTGIAVPANHVNFQV
                     EVTLAAMFGGVFDGTTNTCSNTYCHFDATTTPWDNPTDLRCQGCHPYATLPDNHGIHI
                     DTFGNICGTCHANTVSGAYTLVDRGKHANLTTEVAIVDSLGGTYSTDTGTCANNWCHG
                     PRNDVVSPTWTGTTDLDCQGCHPTASLSVGHPFHISTMGQQCQNCHNATAVSPTELTV
                     DGPHVDGTVQV"
BASE COUNT          695 a         1113 c         1114 g          762 t
ORIGIN      
        1 cgacctgcac cgtcccgtcg acgtgcggcc cgtcaaccgt tagctccgtc ggcgatacgg
       61 cggtcgcatt atggcagttc tgacactgct ggcccatcgt cgatatgtgg aatggatgtc
      121 cgacggagag actcgccgtc gggtgacacc cttggcaatc gagatcggtc gttccggtcc
      181 acgtcggact gacgacgtcg ttgcgcggtc cgtgacacca gttgttcgcg cacgtgcccg
      241 tatccgtcga atacgtaccg ccaagtgaat cgacaattgc aacctcggtg gtgaggttcg
      301 cgtgtttacc gcgatcgacg agcgtatacg caccgctcac cgtgttcgca tgacacgtgc
      361 cgcagatatt cccgaacgta tcgatatgga taccgtgatt atccgggaga gtcgcgtacg
      421 gatggcaccc ctgacaccgc agatccgtcg ggttgtccca cggcgttgtc gttgcatcga
      481 agtggcaata ggtgttgctg cacgtgttcg tcgtgccgtc gaagacaccg ccgaacatgg
      541 cggcgagcgt cacttccacc tggaagttca cgtggttcgc cggcacggca attcccgtgg
      601 aatcctgcgc cgtggatgcg tgacacgtct cgcagttctg ctccatgctc ttgaagtgcg
      661 ccggatgcga aaccggtagc tcggcgacgg catgacaact gttgcagtcg agcgtagagg
      721 tcgtccacac cggcgtgccc gaccggtggc aatacgtatc cgcgcacgag ttcgtctgag
      781 tgtcgtatgt cccttcgacg acatcgttca tggccacttg tacgatctga tcggtatgga
      841 gcgacggatt gatcatcgta tacggaccgc tcaccgtctc gctgtgacac accgagcagt
      901 cgttcgaaac gagatcgacg tgcaccgggt gcggttccgg taagctcgcg tacgaatggc
      961 atgattggca ccgcagatcg gtcgtgccgt tccatgcagg tgtaggcgtc gcgtcgagat
     1021 ggcagtacgt gtcactacac gtaccagtcg ccgaatcgta cgtaccgtcg attccgtcga
     1081 tcatcgcgac atcgacagcg tagttcatgt gcaacagcgg atccttgatc gccgacgagt
     1141 ccgtcgccgt cttcgcgtga cacgtctcgc agttcgccgg agaccgcgcg aagtgcgcct
     1201 gatgcgcgcc cggtagctca gccttcgcgt ggcagctgtt gcagtcgagc gacagccccg
     1261 cggaccacgt cggcgcgccc gatacgtggc aatacgtgtt cgagcacgag ttcgtcacgt
     1321 cgtcgtacac cgcaccgacg ccggcctgag cgttcatcgc cacctgcacg atctggtcgg
     1381 cgtggagcga tggattgatc atcgtgtacg ggccactcac cgtctcgctg tgacataccg
     1441 aacagtcgtt cgaaacgaaa ctcacgtgcg gcggatgggg gtcagccaaa ctcgcgtacg
     1501 agtgacacga ctggcaccgg aagaccgttg agccgctcca tgcaggagtc ggcgtcgcat
     1561 cgaggtggca gtacgtgttg ctgcacgtct cggtagtcga gtcgtacgtg ccgtcgatac
     1621 cgtcgatgag cgcgacatcg atcgtaaagt tcatgtgtaa cgacgggtcc ttgatcgccg
     1681 acgaatccgt cgccgtcgat gcatgacacg tctcgcaatt tgccggagac cgtgcgaagt
     1741 gcgcctgatg cgcacccggc aggtccgcct tcgcatggca gctgttgcag tcgaggtcga
     1801 caactgcggt ccacgcaggt gattgcacac cgtgacacga cgtatttgta cacgtctgcg
     1861 tcgcggacac gaactcaccg ttgatcgtcg tgctcagcac aacgtccgtt tggagattcg
     1921 cgtggaaagc cggattcgct atagccgcgt tactcgctgc cgttatcgcg tgacacaatg
     1981 agcaatcgtc gtttttcgcg aggtgcttcg catgtgcgtc cggtaactcg gcgaacgcat
     2041 ggcacgattg gcagtccaga tcggtggttc cggtccacgc aggtgtcgtt acacccacgc
     2101 cgtgacaata cgtatcggaa catgctccgg ccgtcgcatc gaacgacccg ccgtgcgagg
     2161 tggcgaacac cacgtccgcc gtgaggttcg catgtgtttc cgtatccggt tgcagttctg
     2221 tcgcgctcac cgcagtggat gcatggcaga tgccgcaatt cgctgcggat tgagcaaagt
     2281 gcgtgtcgtg cttgtccgaa aggtccgcga tcgcgtgaca gctctgacac gtaagatttc
     2341 ccgtggctcc ccaagctggt gacgtgttgg gccgggtaat cccgtgacag cgtgaatcgc
     2401 tacacgtcat gtcgtcggcg ttgaaggtcc cttcgacggt aacgctcagt gcaacgttga
     2461 gcaccgcgtt catatggacc gaatcaagta gcgcgccggt cgtgctaaac gttgcggcgt
     2521 gacagagcgc gcagccgccg gcacccgcag caaagtgctc ctgatgggac ggcggaagct
     2581 ccgaacgctc atggcacgtt ccgcaactca aactcgttgc cgtctccgac cgtgcatgac
     2641 atcccggcga gttcgcgcac gaaatcgcga ttgcctcggc agtgctgtcc gccgcgacga
     2701 ccgtgtgaca ctccagacac gaggcatttc caccaaaatc ggcggcgcgc acgcgtgccc
     2761 cgtggaaatt ctccgacgtt gcgtcgacga atccggccgg atgcggtcca cgtcggatcg
     2821 ggtcggatgt tttctccgaa cacgcaacga ataccgctcc aatcgccagc atcgcgcaga
     2881 gcattccgat ataccgcatg ataagcgttc ctcgcgagta tcgtcgaatg tcgtgactcg
     2941 gcctcatcat cgctcagttc ccgattcccg tgtgacagcg aatacacgac ggtgatgaac
     3001 cggtgtgaca tgcggagcac gcctcgaggt ccgtcgcggc gaaaccggcg tgatcgccgc
     3061 catcaggctt gacccaattc gagaacgaat ggttcgccgg catgactcgg cgtacctgat
     3121 ggcacgtaac gcacgagctt gcgaagtcat ggcacgcggc gcattccttc tgcttcgcgt
     3181 agaagctgaa tgcgtgcatg ctttggaatc cctcgcgatg gggtattcca cgcaagtcgt
     3241 caccgcggtg acacgcgtcg caatcctgct cttgaacatg acacgacgca cacgtcgcgt
     3301 tatcgacccg cgcgacctcg ccgtggagcg tgttccactg cgggacttcg tgcgtttggg
     3361 gaagaagcgt caacaacgcg gtcggcgtat ggcaggtctc gcaggtgatc tcggctttta
     3421 caccgttgtg gcaatcggta caggtctcca tgggcggcat tccgaaaacg atctgggcat
     3481 ccgcgctcac accgacgcgc gtatgacacg tttcgcagtc gacgcctctg tcagcgtgaa
     3541 tcttgtgcgg gaagaagtcg acgttacggt gcgggacacc taccgatacg gcattgtccg
     3601 cgtccgtatg gcacgtctcg cagtcatccg gcgcgccgat tccgtcatgg caatcgaagc
     3661 aggtgtcgtg gtccggaatg agtc
//
LOCUS       DSCC01000401            3414 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018779, whole genome shotgun sequence.
ACCESSION   DSCC01000401 DSCC01000000
VERSION     DSCC01000401.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018779"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..945)
                     /locus_tag="ENN56_04955"
     CDS             complement(<1..945)
                     /locus_tag="ENN56_04955"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HDS74874.1"
                     /translation="MVHESLSKRTDQPNVIFIMTDQERYDTIGALGFPWMKTPNLDRL
                     MREGVSFDRCFCTAPSCAPSRASLFCGDWPHRAGVLRNGHHWQLPSWIEQFRDAGYDT
                     INLGKMHTQPVDDACGFTQRFIVENKDRPLWASVGSYYYDEWQRFLFQNGVKFPDREW
                     YRSEHPDYATGLGAYPWPLEERYHPDRYVGDLACWFIDQWPSEEPLFLQIGFPGPHPP
                     YDPPQRYIDMYDDVDIPVPDVTREELDGQPWPQQSYRKEMVEGNHDAVKWHDQPSKEQ
                     LRRLRKYYAANMTLIDEQIGRIIEKLRKKGYLDNAIIVF"
     gene            complement(996..2414)
                     /locus_tag="ENN56_04960"
     CDS             complement(996..2414)
                     /locus_tag="ENN56_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDS74875.1"
                     /translation="MATRIEKDSLGEMSVPEAALYGAQTARAVENFPISGITFPRSFI
                     KALGQIKHAAASVNEDLGLLDSKVADAIRAASQEVIDGKLDEHFVLDVYQTGSGTSTN
                     MNANEVISNRAIQMLGGTIGSKDPVHPNDHVNMSQSSNDVIPTAIHVAAYNEAVAHLI
                     PGMEHLAKTLRKRAKETSDVVKTGRTHLMDAMPITLGQQIGGHATQVEHGVERVKSAL
                     PRLAELPIGGTAVGTGINAHPEFARRMAVKLSELTGNPFVEARDHFEAQAAQDAAVEL
                     SGQLKTVAASMMKIANDYRWMNSGPQTGLMEIVLPATQPGSSIMPGKINPVIPEAVTL
                     VAAQVIGNDVAVTIGGASGNFELNLMLPLIAHNLLGSILLLGNACRVLADKAIAGFQV
                     NREHTEDLVDKNPILATALNTKVGYDTAAKIAKKAMAENRKVRDVAKELTDLSDAELD
                     ELLDTAAMTRGGIPGGGMASGG"
     gene            2566..>3414
                     /locus_tag="ENN56_04965"
     CDS             2566..>3414
                     /locus_tag="ENN56_04965"
                     /inference="COORDINATES: protein motif:HMM:PF04170.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74876.1"
                     /translation="MKLRILGVATAVLVVVAACAKTVEPPFPDVSQPIPVLLTTYSGT
                     LPCADCPGIVVELTLATDAHTGEPIQYREHRTYLEADENGDVTVVETGTWEIHRTGEP
                     FSERVVYHLRPDQSTVVFRYRAITNTLRLLDQTGNDIESLLNYALVRSDTVHILLGDH
                     AIISAPALYGDSTGAALDVRSDGSFVLRGIIPRPLFGKWSVSERGDTLLLDAGCGNLY
                     RLGLSDDSTLVMLDENDEVTQSLDLRSPYEPIDDTLRLMGMYSYFADAGWFTECHTGN
                     RYPVAQL"
BASE COUNT          660 a         1051 c          953 g          750 t
ORIGIN      
        1 gaacacgatg atcgcattgt cgagatatcc ctttttgcgg agcttctcga tgattcgacc
       61 gatctgttcg tcgatgagcg tcatgtttgc ggcgtagtat ttccgtagac gccgaagttg
      121 ctctttcgac ggctgatcgt gccactttac cgcatcgtgg ttgccttcga ccatctcctt
      181 gcgatagctc tgttgcggcc acggctgccc gtcgagttcc tcacgcgtca catccggcac
      241 gggaatatcg acgtcgtcgt acatgtcgat gtagcgttgc ggcgggtcgt acggcggatg
      301 cggcccgggg aagccgatct gcaagaacag cggctcttca gatggccatt gatcgataaa
      361 ccagcacgcg agatcgccaa cataccggtc cggatggtag cgctcctcca gcggccacgg
      421 atacgcaccg agaccggtcg cgtaatcggg atgctcggac cggtaccatt cgcgatcggg
      481 gaacttcacc ccattctgga agagaaagcg ctgccactcg tcgtaatagt agctaccgac
      541 cgatgcccag agtggacggt ccttgttttc cacgataaag cgctgcgtga atccacacgc
      601 gtcatcgacc ggctgcgtgt gcatcttgcc aagattgatc gtgtcgtatc ccgcgtcgcg
      661 gaattgctcg atccaactcg gcaattgcca gtgatggcca ttccgcagca ctcccgcacg
      721 gtgcggccaa tcgccacaga acaagcttgc gcgcgacggt gcgcacgagg gagcggtaca
      781 gaaacaccga tcaaagctca cgccttcacg catgagccgg tcgaggttcg gcgtcttcat
      841 ccacgggaag ccgagtgcgc caatagtatc gtaccgctcc tgatcggtca taatgaaaat
      901 gacattcggc tgatcagtac gcttcgataa tgattcgtgt accatagaca cctccccgct
      961 gatgaattgt ggaccggctc cctcagtaga cccgatcaac cgcccgacgc catgccgccg
     1021 ccgggaattc cgccccgagt catcgctgcc gtatcgagca attcgtcgag ttccgcgtcg
     1081 gaaagatcgg tgagttcttt cgccacgtcg cgcaccttgc ggttctccgc catggccttc
     1141 ttcgcgatct tcgccgccgt gtcgtatccg accttcgtat tcagtgccgt cgcgagaatc
     1201 ggatttttgt ccacgaggtc ttccgtgtgt tctcgattca cttggaagcc cgcgatcgcc
     1261 ttatccgcga gaacacggca cgcattaccg agcaaaagaa tcgagccgag cagattgtgc
     1321 gcgatgagcg gcagcatgag gttcagttcg aagtttcccg atgccccacc gatcgtgacc
     1381 gcaacgtcgt tgccaatgac ctgcgccgca accaacgtca ccgcctcggg aatcaccgga
     1441 ttgatcttcc cgggcatgat cgatgagccg ggctgcgtcg ccgggagcac aatctccatg
     1501 agccccgttt gcggacccga attcatccag cggtagtcgt ttgcgatctt catcatcgac
     1561 gcggcgaccg tcttgagctg accggaaagc tcaaccgcag cgtcttgcgc cgcctgcgcc
     1621 tcgaagtggt cgcgcgcctc gacgaatggg ttgccggtga gttcgctcaa tttcaccgcc
     1681 atgcgccgcg cgaactccgg atgcgcgttg atgcccgtgc caactgccgt tccgccgatc
     1741 ggaagttcgg cgagacgggg cagcgcagac ttcacgcgct cgacgccgtg ttcgacttgc
     1801 gtcgcgtgcc cgccgatctg ctgaccgagc gttatcggca tcgcgtccat gagatgggtg
     1861 cggcccgtct ttacgacgtc gctcgtctcc ttggcgcgct tccgcagtgt cttcgcgagg
     1921 tgctccatgc cgggaatcag gtgcgcgacg gcctcgttgt acgcggctac gtgaattgcc
     1981 gtggggatca cgtcgttcga cgactggctc atgttcacat ggtcgttagg atgcacggga
     2041 tctttcgagc caatcgtgcc accaagcatc tgaatcgcgc gattcgaaat cacttcattg
     2101 gcgttcatgt tcgtcgatgt gcccgagccg gtctgataca cgtccaacac aaagtgctcg
     2161 tcgagttttc cgtcgatgac ctcctgcgaa gcggcacgaa tcgcgtcggc aaccttcgaa
     2221 tcgagcagac caaggtcttc gttcacgctc gccgctgcgt gcttgatttg ccccagcgcc
     2281 ttgatgaacg accgcgggaa cgtaatcccg ctgatcggga aattctcgac cgcgcgcgcc
     2341 gtctgcgcgc cgtagagcgc cgcttccggc actgacatct cgccaagcga gtctttttcg
     2401 atccgtgtag ccataagatg attcctcacg tgaatatcga tgaatgaccg tttccgcctg
     2461 aacagacgtt tcatacacta aacgtaggca aaatcggtgt gcgaacgcga ggtaccaaac
     2521 cggtatccgc gcagtagctg caccgtttta atgggatgag ttaccgtgaa actccgcata
     2581 ctgggtgtcg ctacagccgt actcgtcgtt gtcgcagcat gcgcgaaaac cgtcgagcca
     2641 ccgtttccgg acgtgagtca gccgataccg gtcctcctga cgacgtattc gggaacgctt
     2701 ccctgcgccg attgccccgg tatcgttgtt gagctgacgc tcgcgacgga tgcacacacc
     2761 ggtgaaccca tccagtaccg agagcaccgt acgtacctcg aagcagacga gaatggcgac
     2821 gtaacagtcg tcgagaccgg cacgtgggaa attcaccgaa caggcgaacc gttttcggaa
     2881 cgggtcgtct atcatctccg ccctgatcaa tccactgtcg tgtttcgcta tcgagcaatc
     2941 accaatacgc ttcgcctgct tgaccaaact ggtaatgata tcgagtcgct tctcaattac
     3001 gcgcttgttc ggtcggatac ggtacacatc ctcctcggcg accacgccat catttccgca
     3061 ccggcgttgt acggcgattc taccggtgct gcgctggatg tgcgctctga cggctcgttc
     3121 gtcctgcgcg gaataattcc gcggccgtta ttcgggaaat ggtcggtatc cgagcgaggc
     3181 gacacgcttc tcctcgacgc gggctgtggg aatctgtatc gactcggact ttccgacgat
     3241 agcacgcttg tcatgctcga cgaaaacgac gaagtaacgc agtcgctcga tttgcggtcg
     3301 ccgtacgagc caattgacga cacgcttcgt ctgatgggga tgtattccta ctttgccgat
     3361 gcgggctggt tcaccgaatg ccacacgggc aaccgctacc ccgttgcgca actc
//
LOCUS       DSCC01000402            3415 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1018771, whole genome shotgun sequence.
ACCESSION   DSCC01000402 DSCC01000000
VERSION     DSCC01000402.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1018771"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            76..537
                     /locus_tag="ENN56_04970"
     CDS             76..537
                     /locus_tag="ENN56_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74877.1"
                     /translation="MKLFGFMILAASAVGTAATTHAAYDPACPPIEDSILSRHWVFSS
                     PIQQRSATGDSTIMHLQTGWDRYGVGKMSDHRSYGWRSIVFWTIGTSVESQVFDVVMP
                     QMVRWSYDPFQTNWFRSEAWKRDPAIRYYLMPNTARDPVRGQPERPARRVD"
     gene            612..1370
                     /locus_tag="ENN56_04975"
     CDS             612..1370
                     /locus_tag="ENN56_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74878.1"
                     /translation="MVKRLALAHLLAYIVAGIAVVAAFTSAVVPNLHDMPAHVRPQIY
                     GTEYAVLLVGAPLLLITTALSARGRIGARALWFGAVAWFLSTYVALATGLGIGPLFPI
                     HTALFFLSLATLVIAFVSIDLDTFGDALPRGSARVGLIGGFIAMIALMAVASRIDPVH
                     ATEQWTAAVLFGIAAFLFGVRSVWAPIIGGIGTVMTSLFGASLIARSGYLGVKGVSIS
                     GASIWLAVAVFFVSSALGIALAITMRPVPDDVET"
     gene            complement(1382..2233)
                     /locus_tag="ENN56_04980"
     CDS             complement(1382..2233)
                     /locus_tag="ENN56_04980"
                     /inference="COORDINATES: protein motif:HMM:PF00795.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDS74879.1"
                     /translation="METFTIAAVQMNALRGELDHNLALHERFTREAAEAGARLVMFPE
                     LSVTAHFGDVTATELAEPANSGRIHDTIHGLAKQHDIIISYGFCEYAHGAYYNSQGLM
                     GPDGLIGVQRKIHASGDEYFTFRMGRSLDTFDLGFCTVGTLICFDSNFFEAWRVLALK
                     GAEVILQPHASRSARGKKLPLTKQKQVIKDVLADTASNSVYAKANSVYAVYANQYGYN
                     GHSTHGGGAYVISPNGEMRVQGVPKLSDLMITAEIDMEELRRIRAARPTLKQRRPEAY
                     GILTEMI"
     gene            complement(2276..>3415)
                     /locus_tag="ENN56_04985"
     CDS             complement(2276..>3415)
                     /locus_tag="ENN56_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74880.1"
                     /translation="DQHNAEVEEIWAAYRRREPIRVPLIFGINARLTMFNHPANPRRI
                     EFAEYSSDPEIMIERQVEHQAWVRENIPQDSPMGRPKDGWHVSVDMQNVYEAGWYGAE
                     IFYRPGQVPDSAPLLQNDDRKWELIERGAPDPFTSGTAAKMWDFYDHMLRRMKQGREY
                     DGLAIVHVDPPAGGSDGPLTICCNLRGAGAFFTDLLDDPDYAQAMLDFVTETTIQRLK
                     AYRERTGQNPKPKEGGLADDSVQLISTPMYRELILPYHRRFFDALYGEGPHSMHLCGA
                     ATRHFRTIRDELNVQSFDTGFPVDFTWLRNELGPDVEILGGPSVPFLETATPDHVTER
                     VKEILGSGIMEGGRFMLREGNNLSPGVSLDNINAMWYAVHEYGRY"
BASE COUNT          640 a          982 c          990 g          803 t
ORIGIN      
        1 atattgccgc gatcgatcca ccggttgacg gcgcgctcat catgcttccg ccccaacgca
       61 ctgccgatgc gctcgttgaa actcttcggc ttcatgattc ttgccgcttc ggcagtcggt
      121 acggccgcaa ccacccacgc cgcgtacgat ccagcgtgtc ccccgatcga agatagtatt
      181 ctcagcagac actgggtgtt ttcatcgcca atccaacagc gcagcgcgac cggcgattca
      241 acaatcatgc atctgcagac tggatgggat cgctatggag ttggcaagat gtccgatcat
      301 cgatcgtacg ggtggagaag catcgtgttc tggacaatcg gtacgtcggt cgaaagtcag
      361 gtgttcgacg tggtcatgcc tcagatggtt cggtggtcgt acgatccgtt tcaaaccaac
      421 tggtttcgtt ccgaggcgtg gaaacgtgat ccggcgattc gctattacct catgccgaat
      481 accgcgcgag atcctgtccg aggtcaaccg gaacgaccgg cgcgacgagt cgattgatgc
      541 agtgattctc tgggatggac ttgccgcgag atcgttcgtg ccgttccctt gataatgagg
      601 agggaatcga catggtaaaa cgcctcgcgc ttgcgcattt gctcgcgtat atcgtggccg
      661 gtattgctgt cgttgcggcg ttcacgagcg ctgtcgtgcc gaatcttcac gacatgcccg
      721 cccatgtacg tccgcagata tacggcacgg aatacgccgt gctcctcgtt ggtgcgccgc
      781 ttctcctcat tacgacggcg ctctcggcac gtgggagaat cggcgctcga gcgttatggt
      841 tcggtgcggt cgcgtggttc ctttccacgt acgtcgcact tgcgacggga ctgggcatag
      901 gaccgctctt ccccattcac accgcgctgt tcttcctctc gctcgcaaca ctggtcatcg
      961 cgtttgtttc catcgacctc gatacgtttg gggatgcact cccacgcggt tcggcgcgtg
     1021 tgggtctcat tggtggattc atcgcgatga ttgcgttgat ggcggttgcg tcgcgtatcg
     1081 atcccgtgca cgcaacggaa cagtggacgg ctgccgtgct tttcgggatt gcggcgtttc
     1141 tgttcggtgt acgaagcgtc tgggcgccca tcatcggcgg tattggtacg gtaatgacga
     1201 gcttgttcgg cgcaagcctc atcgcgcgaa gcggatatct cggcgtgaaa ggtgtctcga
     1261 tttccggggc gtcgatttgg cttgctgtgg cggtgttctt cgtttcctcc gccctcggaa
     1321 tagcgctcgc tatcaccatg cgacccgttc ccgacgacgt tgaaacgtag acccgctacg
     1381 gttagatcat ctcggtgaga atcccgtacg cttccggtcg gcgttgtttg agcgtcggtc
     1441 gagcggcgcg aatacgccga agctcttcca tgtcaatctc cgccgtgatc atcagatcgc
     1501 tgagcttcgg aaccccttgt acacgcattt cgccatttgg cgaaataacg taagcaccgc
     1561 ccccatgggt gctatgaccg ttatacccat attgattcgc atacaccgca tacaccgaat
     1621 tcgctttcgc gtagacgctg ttcgacgcgg tatccgcaag cacgtctttg ataacctgtt
     1681 tctgcttcgt gagagggagc ttctttccgc gagccgagcg ggaggcatgc ggttggagga
     1741 ttacctcagc gcctttcagc gcgagcacgc gccacgcttc gaaaaagttc gagtcgaagc
     1801 agatgagtgt gccgacggtg cagaatccga gatcgaacgt atcgagactg cgtcccatgc
     1861 ggaacgtgaa atactcgtcg cccgacgcgt ggatcttccg ctgtacaccg atgaggccat
     1921 ccggtcccat gagtccctgc gaattgtagt acgcgccgtg agcgtattcg cagaaaccgt
     1981 aggagataat gatgtcgtgc tgtttcgcga ggccgtgtat cgtatcgtga atacgacccg
     2041 aattggcggg ctcggcgagt tccgtcgcgg tcacgtcgcc gaaatgcgcc gtgacggaaa
     2101 gctccggaaa catcacgaga cgcgccccag cttcggcagc ttcacgggtg aatcgttcgt
     2161 gcagtgcgag gttgtggtcg agttcgccac gtagggcgtt catctgcacc gctgctatcg
     2221 taaacgtttc catacggacc tcttcgcgtg taactccatc tgactctacc gatactcaat
     2281 agcggccata ctcgtggacg gcgtaccaca ttgcgttgat gttgtccagt gaaacacccg
     2341 gcgagagatt attcccttcg cgcaacatga atctgccgcc ttccataatt ccgctgccga
     2401 ggatttcctt cactcgttcc gtgacgtgat ccggagttgc cgtttcgagg aacggtacgg
     2461 acggtccgcc gagtatctca acatcgggac caagttcgtt gcgcaaccac gtgaaatcaa
     2521 cggggaatcc ggtgtcgaag ctctgcacgt tcagctcatc gcggatggtg cggaaatggc
     2581 gggtcgcggc accgcagagg tgcatactgt gcggtccttc accgtagagt gcatcgaaga
     2641 accggcggtg atagggaagg atcaactcac gatacattgg tgtgctgatg agctgcaccg
     2701 agtcgtcggc gagtccgcct tctttcggtt ttgggttctg accggtacgc tcgcggtatg
     2761 ctttgaggcg ttgaatcgtt gtttccgtaa cgaaatcgag catcgcctgc gcatagtccg
     2821 ggtcgtcaag cagatcggtg aaaaacgcac ccgcaccgcg taggttacag cagatggtca
     2881 gcgggccatc gctgccaccg gccggcggat cgacgtggac tatcgcgaga ccgtcgtact
     2941 cgcggccctg tttcattcgc cggagcatgt ggtcgtagaa gtcccacatt ttcgcagcgg
     3001 taccgctggt gaatggatcg ggcgcaccgc gctcgatgag ttcccacttt cggtcatcgt
     3061 tctgaaggag cggtgccgaa tcgggtacct gccccggccg atagaagatc tcggcaccgt
     3121 accagccggc ctcgtagaca ttctgcatat ccacgctgac gtgccaaccg tccttcggcc
     3181 ttcccatcgg cgagtcttgt gggatattct cgcgaaccca cgcctgatgc tcaacctgtc
     3241 gctcgatcat gatctcggga tcggatgaat actcggcgaa ttcgatacgg cgaggatttg
     3301 ccggatgatt gaacatggtc agtcgcgcgt tgattccgaa gatcaacggc acacgtatcg
     3361 gctcacgcct tctatacgcg gcccagatct cttcgacttc tgcgttgtgc tgatc
//
LOCUS       DSCC01000403            3050 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1021875, whole genome shotgun sequence.
ACCESSION   DSCC01000403 DSCC01000000
VERSION     DSCC01000403.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1021875"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            377..844
                     /locus_tag="ENN56_04990"
     CDS             377..844
                     /locus_tag="ENN56_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74881.1"
                     /translation="MNYPQPTSVENVLEILSLALKAEDNPAYLERLSESFQFVPDPVQ
                     LASPAFRNFPTEWTIDHEEAFLAGLLSNADSVLVAWQSVFTLLEGETATATATYELRV
                     VTQVSEITMYRGQAEIVARQTAGVWNIETWRDVQVGGYTNTWGLLRAMLLAAG"
     gene            887..1576
                     /locus_tag="ENN56_04995"
     CDS             887..1576
                     /locus_tag="ENN56_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74882.1"
                     /translation="MGGRLRKLLSGFRGDSSHCTRARLAMLMFAGSISLFACDLLNQT
                     PIIREGGFAAKQAPLSLATPDSVVKMLVYIFDRHTPETARQFADLLAPGYVYRYDDPT
                     DQNQLELDRASEIRVYENIFRNFETISAEFIVESRWTEYGSAMTYPAGTTTRYISERH
                     PNENWTVLQVIGDMEFSNTDEQARIVGYRVFQRFELGFRFASATPDSTWQLASWTDRE
                     SLISARITHGE"
     gene            1593..>3050
                     /locus_tag="ENN56_05000"
     CDS             1593..>3050
                     /locus_tag="ENN56_05000"
                     /inference="COORDINATES: protein motif:HMM:PF00912.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74883.1"
                     /translation="MFYSAPTICYLISRYDSIITDTREVSVLRTFVVRLAIVAGVFLV
                     VFTTTLVFLVREFSTDLPDLAALERIRPKLTTSVYSSDGQVLKTYSIQRRTLIPYDEL
                     PPALIDALIASEDRQFFRHWGINVLGLARAVLVAVRKREGPSATSTITQQLARDLFLT
                     KERSLVRKFKELILAVRIEQTYTKEEILQLYLNQVYFGGGAYGIEAAAEAYFDKHAKD
                     LSTIEAGTLVGILPAPNLYHPIRREDRALTRRNIVLWAMGQTGSLSQDSVALLQLQPL
                     ETKPPEEEIGLAPYFTEYVRLTLTDRVFRDDSTRVRYAQQLGLPDSVSVDDLVYEGGL
                     VIETTLDSRLQVIAERVLSNHLRELQARIDTLAQAYPDSFPEYVDRYVFRVDSVTGDT
                     LGIDSVFVKKFQAALVSIDVQNGAVRALVGGRDFEESKFNRAVQALRQPGSAFKPFVY
                     TAAIDNGWSPIDQLPNQPITITEMVDDEWREWRPQN"
BASE COUNT          660 a          785 c          856 g          749 t
ORIGIN      
        1 gcgttgtttc gtcgtcacta tcggatgatc acgtatgtcg cgactgcgat tcttgtcgga
       61 ctcgtgttcg tttcggcggt atggacgtta ttcgcgattc ttgcattgat gttcggacgt
      121 aaacatccgc cgccaaccga tgacacgacg ccgatcgata ctggtcggcg actcgttggg
      181 tatctttgta tcgtgattct tgtgctctgt tttatgccga atccattgaa tgtcgtcggt
      241 ctgtaagatg aagagacaca cgcgacagtt tttggtgcca tctttccttg tcgttttctg
      301 tgttactgca acgctattca cgggttgtaa gaatcccttt gcaacgggta ccccggaagc
      361 gccgggaggc gctttagtga attacccaca accgacaagc gtcgagaatg tgttggagat
      421 tctctcgctc gcgttgaagg cagaggataa tccggcgtat ctcgaacggc tatctgaatc
      481 gtttcagttc gttcccgatc cggtgcagct tgcgtccccg gcgtttcgaa actttcctac
      541 ggagtggacg atcgatcatg aggaggcttt tctcgccgga ttgctcagta atgccgattc
      601 ggttctcgtc gcgtggcaga gtgtatttac gttgctcgaa ggcgagactg cgaccgcaac
      661 agcgacctat gagcttcgtg tggttaccca ggtgagcgag ataaccatgt atcgcggaca
      721 agcggaaatc gttgcgaggc agacggcagg cgtttggaac atcgagacat ggcgcgatgt
      781 tcaggtaggt ggatatacga atacatgggg cttactgcgc gcaatgctgc ttgcggccgg
      841 ctgaaccgag tgaaggtgag catcggcaaa caccagagga acgccgatgg gcgggcgact
      901 ccgaaagcta ctgagcggat tccgcgggga ttcgtctcac tgtacgcgtg cgcgactcgc
      961 aatgctgatg ttcgctggaa gtatttcgct tttcgcgtgt gacctactca atcaaactcc
     1021 gattatccgt gagggcggtt tcgccgcgaa gcaagcaccg ttaagtcttg ctacccccga
     1081 cagtgtcgtg aagatgctcg tgtatatctt cgaccgacac actcccgaaa ccgcacgtca
     1141 gttcgccgac cttctcgctc ccgggtacgt gtaccggtac gatgatccga ctgatcagaa
     1201 ccaacttgag ctcgaccgcg cgagtgagat tcgcgtctac gaaaacattt ttcgcaactt
     1261 cgagacgata tcggcggagt tcattgtgga atcgcggtgg acggaatacg gatcggccat
     1321 gacgtatccc gcgggaacga cgacgcggta tatctctgaa cgacatccaa acgagaactg
     1381 gacggtactt caggtgattg gtgacatgga gttctcgaat accgacgaac aagcgcgcat
     1441 tgttggttat cgggtgttcc agcgattcga gctcggtttc cgttttgcgt ccgcgacacc
     1501 ggactctaca tggcaacttg cgtcgtggac cgatcgcgaa tccttgatca gcgcgcgaat
     1561 cactcacggg gaatgaagcg aggcgccgtc cgatgtttta ctcggcgcca acgatctgct
     1621 accttatatc tcgttacgat tcgattataa cggatacgag ggaggtttca gtgcttcgta
     1681 cctttgtggt gcgacttgct atcgtggccg gtgtatttct cgtggtattt actaccacgc
     1741 tcgtttttct cgtacgagag tttagcacgg atctccccga ccttgccgct cttgaacgga
     1801 ttcgcccgaa actgacgacg tcagtgtatt ccagcgacgg tcaggtgttg aaaacgtact
     1861 cgatccaacg ccgcacgctg attccctacg acgagttacc gccggcgctt attgacgcac
     1921 tcatcgcgag cgaggatcgg cagttctttc gacactgggg tatcaatgtt ctcggtttgg
     1981 cacgtgcggt gcttgttgcc gtgcgcaagc gagagggtcc cagcgcgaca agtaccatca
     2041 cacaacagct tgcgagagac ttgtttctca cgaaggaacg tagcctcgtt cggaaattca
     2101 aagagttgat cctcgccgtt cgaatcgagc agacatatac gaaagaggag atactccagc
     2161 tctacctcaa tcaagtgtat ttcggtggcg gcgcgtatgg aatagaggct gccgcggagg
     2221 cctatttcga caaacacgcg aaagacctga gcaccataga agcggggaca ctcgtgggca
     2281 ttcttcccgc gccaaatctc tatcatccaa tacgtcgtga ggatcgcgct cttacacggc
     2341 gtaatatcgt tctctgggcg atgggacaaa ccggttcact ttcgcaagac tcggttgcac
     2401 tgcttcaact gcaacctctg gagacgaaac cgccggaaga ggaaattggt cttgcaccgt
     2461 atttcaccga gtatgttcgg ttaacgctta ccgatcgtgt gttccgagac gattcgacgc
     2521 gagttcggta tgcacagcaa ctcgggctgc ccgattcagt aagcgtagat gatcttgttt
     2581 acgaaggcgg actcgtcatt gaaacgacgc ttgacagcag attgcaggtg attgccgaac
     2641 gcgtgctttc gaatcacctt cgcgaactac aggctcgtat cgatacattg gctcaggcgt
     2701 atccggactc atttccagag tatgtcgatc ggtatgtctt tcgcgttgac agcgtgaccg
     2761 gcgatacgct tggcatcgac agcgtattcg tgaaaaagtt tcaagcggca ctcgtttcga
     2821 ttgatgttca gaacggagcg gttcgcgctc tggtcggtgg gagagatttc gaagagagca
     2881 agttcaatcg tgctgtacag gcgcttcgcc aacccggctc cgcgttcaag ccgtttgtct
     2941 atacggcggc tatcgataac ggctggtcgc cgatcgatca gttaccgaat cagccgatta
     3001 cgataaccga aatggttgac gatgaatggc gggagtggcg gccgcaaaac
//
LOCUS       DSCC01000404            2725 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025388, whole genome shotgun sequence.
ACCESSION   DSCC01000404 DSCC01000000
VERSION     DSCC01000404.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025388"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            1531..1968
                     /locus_tag="ENN56_05005"
     CDS             1531..1968
                     /locus_tag="ENN56_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74884.1"
                     /translation="MKIKEEALRKWAENDLTMTQLPQGGYDALFSYRATTCRNGGTEF
                     DSALRITLHPENGDWRIDNVAVEIDPNDPGWKQTCIHESSANPNPATLAKHSQARGML
                     VNDFLERDWPTDNAGCYCTSIHLTHKLILAVSTVRYWLNNHTK"
     gene            complement(2022..>2725)
                     /locus_tag="ENN56_05010"
     CDS             complement(2022..>2725)
                     /locus_tag="ENN56_05010"
                     /inference="COORDINATES: protein motif:HMM:PF13088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neuraminidase (sialidase)"
                     /protein_id="HDS74885.1"
                     /translation="GFFIKSNDDGKTWSDPEIMPAGLFGPIKNKPIRLADGRIACGSS
                     VESYNAWTGWIHIYDPSTDQWSIRGPLAVPGNQRGLIQPTLWESEPGHVRAYLRSTRE
                     IGYICVSDSVDNGETWSAAEKTALPNPNSGIDAVKLDDGRVVLIYNHTQRTDSQGGRE
                     IIHAAVSPDNGTSWSAPWVIEDEGKEYSYPAIIQGSDGTVHVTYTWNRVGVRYVAFSR
                     EEIDRVTDGTLQRAR"
BASE COUNT          654 a          797 c          690 g          584 t
ORIGIN      
        1 acatgggtta ccgtattgtc atcatacctc catcggtgaa tattcgctca tcgacgcttg
       61 agttacttaa gtcgtttacg tcggctggcg gaaccgtatt cattgtcgga tctatgccgc
      121 acctcgtcga cggtaagccg tcggaagaag cccgttggtt cctctccgaa cacgcgcatc
      181 gagtcgatgg tgccgaacgt ttcgactact cagagatcat cgagtccatt tccgacgcca
      241 acggtcggac ggttgttgtc cgaaacatcg atggcaccga tacgccacaa ctcaaagtga
      301 accgccggat ttggaacgga tgcgagatac tgtatctcgc gaacgtatcg cgcgaccaca
      361 tcactgcatc cgtcacattc gatttcgcgg tcaatggagt catcgaagag tgggacctat
      421 caacaggtac aacgactcca ttcgcaccgt gttcaatatc cgagccgtgc atacatcaag
      481 tgtcgtggca ccctcggcaa gcacgcgcgt tcgtcgcggt accacgctcc gtcgatatgc
      541 cgccaccgcg gaaatggcaa gaggctcgtc gccttcaacc gagttggacg ggaaaacgca
      601 ctgaaccgaa cgcactgttg ttggataccg ttatccgcgt aaaaggcgac atgagccaac
      661 cgactcagtc aatatcggac gcacagaacg atctgtcgaa gagaaatgct tcggacacga
      721 cgaaaccgat cacgttcgaa taccccttcg aggtaaatgc ggaagcgata cctaccggca
      781 acctgcgtat tgcgggagag tttgaaggca attcgaagat cacactcaac gatcaccccg
      841 catccccgct cccgaatacg tggtttctcg atccggcgat acgcgttgtt ggagcagaac
      901 gagtcgtgcg agacaacaat acacttcgca tcacgggtgt ttttgagcaa ccggaatcgc
      961 tacagtcggc gtttcttctc ggcgacttcg atgtcgcgac gagcgacgac gtacacttcg
     1021 ccatcaccgc tccacgagaa cggatcccga tcggaccatg gccggaagtc ggccttccgt
     1081 tttatgcggg aactgtcgtg tacacgacgg ttatcgacct tcgctccgaa gccggttcac
     1141 gaatcattct cgacctgccc gggcttgtcg gcgcggcgga gatacgggta aacggtgcga
     1201 tcgttgatca cattctttgg ccaccgtact cttgcgatat ctcagcgaac actcacgatg
     1261 gagaaaacgt aatcgaaatc gaagtggcga atacgctccg taaccttctc ggcgcgcact
     1321 acaattacaa tgaagagaca caagcaggga tatccattgc gtcgtatagt gccccgtacg
     1381 gcgcaaaaaa gcaattcaaa gcctacggta tgcttagcgc accggagata gtgatagagc
     1441 gggctgagta acaagagttt ttcgcattgg aacactcaat gtacggtact tgctgtatac
     1501 ttgatctctt taggagggtt ggcgaaggat atgaagatca aggaggaagc gttacggaag
     1561 tgggcagaaa acgatctcac catgactcag ctcccacaag gcggatatga cgcgttgttc
     1621 tcctatcgcg caactacgtg tcgaaacgga ggaaccgaat tcgattccgc gctgagaatc
     1681 accctccatc cggaaaatgg cgactggcga atcgataacg tcgccgtcga aatcgatccg
     1741 aacgacccgg gctggaagca aacatgcatt cacgaatcca gcgcgaatcc taatccggcg
     1801 acgcttgcga aacactcgca ggcgcgaggt atgctcgtga acgatttcct cgaacgcgat
     1861 tggccgacgg ataacgccgg ctgttactgc acctcaatcc atctcacaca taaattgata
     1921 ctcgccgtat ccaccgtgcg gtactggcta aacaaccaca ccaaataaga aaagcggggc
     1981 gacgttccac acgctgcccc gctttcgcat gtacacgacg ttcatcgtgc ccgctgcaac
     2041 gtcccatccg tcacacgatc gatctcttca cgtgagaacg ccacgtatct cacaccaacg
     2101 cggttccacg tatacgtcac gtgaaccgta ccatcggaac cttggatgat cgccggatat
     2161 gagtattctt tcccctcgtc ttcgataacc cacggtgcgc tccacgacgt tccattgtcc
     2221 ggcgaaacag ccgcgtgaat tatctcgcgc ccgccctgac tgtccgttcg ttgtgtgtga
     2281 ttgtagataa gcacaacccg gccatcgtcg agtttcaccg catcgatacc gctgttcgga
     2341 ttcggtaacg ccgtcttttc agcggcactc catgtctctc cattgtccac cgaatcgctt
     2401 acgcagatgt agccgatttc cctcgtggaa cggagatacg cgcgaacatg ccccggctcg
     2461 ctctcccaca acgtcggctg aatcaatccc cgttgattac ccggaaccgc gagcggccca
     2521 cgaatcgacc actgatccgt ggagggatca tagatgtgaa tccaaccggt ccacgcgttg
     2581 taactctcga cggaactccc gcacgcgatt ctaccgtcgg caaggcgaat cggcttgttc
     2641 ttgatcggac cgaacaatcc ggcgggcatg atctcgggat cactccacgt tttcccgtcg
     2701 tcgttggatt tgatgaagaa accgc
//
LOCUS       DSCC01000405            1753 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1046030, whole genome shotgun sequence.
ACCESSION   DSCC01000405 DSCC01000000
VERSION     DSCC01000405.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1046030"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="ENN56_05015"
     CDS             complement(<1..1201)
                     /locus_tag="ENN56_05015"
                     /inference="COORDINATES: protein motif:HMM:PF13229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HDS74886.1"
                     /translation="MTKYTRLMPTDTNERPFTMKRRSVYAVALFAALGIGIGCASTGG
                     RTMQTVTEYHVSPTGDDRCSGERDMPFRTLAHARDMIRHARTAGIPSPNGVTVWLHDG
                     RHELDETFVLGPEDSGTPNAPIIYRALPGANVTISGGREIGGWTDYAGDDAAVTGIKQ
                     TSIAHLGFGESDMVGTEHVFPFELFYRGERMDQARWPNRDPEDIYSGWAHIASVPEPE
                     QRLQFGVRVDAEQLRGWATAEHAQFHMYARYDWSDVYVGLGRVDVEAAQIHLADPTNY
                     TIATGRRYMIRNVFSELDIPGEWYVNARDGVVSFLPPDGLEDGDVIVSRVDELVHIDG
                     ASHIVFRDLRFEHARDAAVVVTSGDGNTIAASTIQNVGGYAVRIDGGTNNRVIGCDIT
                     KTGTGGFV"
BASE COUNT          347 a          563 c          481 g          362 t
ORIGIN      
        1 gcacgaagcc cccggtacca gttttggtaa tatcgcagcc gataacgcgg ttattcgtgc
       61 ccccgtcgat tcgcactgcg tatccaccga cgttttggat cgttgacgcc gcaatggtgt
      121 tcccgtcgcc gctcgttacg acaaccgccg catcacgtgc atgctcgaac cgaagatcgc
      181 gaaaaacgat gtgcgaggcg ccatcgatat gcacgagctc atcgacccgg ctcacaatga
      241 cgtcgccgtc ttcgagcccg tccggtggga ggaaggaaac gacgccatca cgcgcgttca
      301 cgtaccactc gcccggtata tcgagctccg agaacacgtt acgaatcatg taccgacgcc
      361 ccgtagcgat cgtatagttc gtcggatcgg caaggtgaat ctgcgcggcc tcgacgtcga
      421 cgcggccgag tcccacgtag acgtcggacc agtcataccg cgcgtacatg tgaaactgcg
      481 cgtgttcagc ggtcgcccat ccacgaagct gttccgcgtc gacgcggact ccgaattgga
      541 gtcgctgttc cggctcgggg accgacgcga tatgcgccca tccgctatag atgtcctccg
      601 gatcacggtt cggccaccgc gcttgatcca ttcgttcacc acggtagaaa agctcaaacg
      661 ggaacacatg ctctgtgccg accatgtcgc tctcaccgaa tccgagatgc gcgatactcg
      721 tctgcttaat gccggtgacc gccgcatcgt caccggcgta gtccgtccaa ccaccgatct
      781 cgcggccgcc gctgatcgtc acattcgcgc cggggagtgc gcggtagata attggcgcgt
      841 ttggcgttcc ggaatcttcc ggcccgagca cgaacgtttc atccagttca tgccgcccgt
      901 cgtggagcca gaccgtgacg ccgttcggtg acggaattcc tgctgtgcgc gcgtgccgga
      961 tcatgtcgcg tgcatgggca agtgttcgga acggcatgtc gcgctcgccg ctgcaacggt
     1021 cgtcgccggt cggcgatacg tgatattcgg tgacagtttg catggtgcgt cctccggtgc
     1081 tcgcacaacc gataccgata ccgagcgccg caaacagtgc gactgcatac actgatcgcc
     1141 gcttcatggt gaatggcctt tcgttcgtat ccgtgggcat aagccgtgta tatttcgtca
     1201 tatcgcgaaa caacggaatg cgataaaatc acgcctccat ggcgaaacta tacagcttcc
     1261 cattgcgtaa tcggaagcgg agatatatcg gctccccgtc gaattggctc atgtcgtcgg
     1321 aaccgttcca cgtaacaaca acgttcggcg cattcgcacg aatcaagtcg cagtcgccaa
     1381 tcgtccgccc ggatgcgacg tcgcccatac gcttcgccgg atcgacatcg tcgggcttcc
     1441 gcacaacctc gaccttcagc tcgccaaatt tgtgctcgtc gccgccattc atcgcgcaat
     1501 tcacgacgag tcgttttccg cgccagatga actctttcgt gaggaggtac ccgtaatcct
     1561 ctgcgagacc ttgcttcggc tccggcgcat cgagcgacat gaatcgatcg atcatgagct
     1621 tgtacaaacc gattgcgcct tcctgctcga acaccttctg cggctgtggg aacccggagg
     1681 tgtagaacca gtactcccaa ccgacgcgga tcgggtcccc tccgatcgat gcgcaaccgg
     1741 catcgaacgc gcc
//
LOCUS       DSCC01000406            1820 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1043843, whole genome shotgun sequence.
ACCESSION   DSCC01000406 DSCC01000000
VERSION     DSCC01000406.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1043843"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..184)
                     /locus_tag="ENN56_05020"
     CDS             complement(<1..184)
                     /locus_tag="ENN56_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74887.1"
                     /translation="MRIEMSLNGRWKFCPAFDTISTDQRWLAPDFDPEHPDLTPQADA
                     VGWIDPGFDDTGWLDIM"
     gene            complement(312..1130)
                     /gene="rsmD"
                     /locus_tag="ENN56_05025"
     CDS             complement(312..1130)
                     /gene="rsmD"
                     /locus_tag="ENN56_05025"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDS74888.1"
                     /translation="MKNVRRLRTFAKFHRRRCAHVVRRIRTETAPKIRHSPPLRCVGH
                     STIPRFQGRIPGPTGAFVKIRVSLFVGKESWETKESEGIVRVIGGIAGSVPLYEVKAQ
                     TTRSMTDRVKMSVFSILDPLLGDAEVLDLFAGTGGLGIEALSRGARFCTFVDRDRTCA
                     RTIARNLDRTKLADRGRILERDSRAAVAQLAAEGLRADIVLFDPPFALGKPPRRIALE
                     RLIASVAERLLAPDGLIVYHHEQDTAGGLFASGLEPVDQRNYGRNIVTFLHLRT"
     gene            1103..>1820
                     /locus_tag="ENN56_05030"
     CDS             1103..>1820
                     /locus_tag="ENN56_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74889.1"
                     /translation="MSAIGERFSSAVSPNKARILMGSTLFWEAQEFSGTMQVRCRDEL
                     ISLAETAGWEYEVAQDVWEGVVVNLNARPERARPMKVQLRTIGVTMFPFNRGENDDRP
                     RLSFVFDNTNLNDPDLRNRLITIAPPAYWHEWMPRVKGQYPALTDEREETIYGLRADG
                     MMRVRRTELPGFIKFLNTLRATALPLLDIRTSDRLARKMINDPNANPFGRNPQPRPLD
                     QATLDEIAEGLGNLFSAPRYP"
BASE COUNT          382 a          525 c          542 g          371 t
ORIGIN      
        1 ccatgatatc gagccacccg gtatcgtcaa agccgggatc tatccacccg accgcatcag
       61 cttgcggagt tagatcaggg tgttccgggt cgaaatccgg ggcaagccag cgttgatctg
      121 tgctgatcgt atcaaacgcc ggacagaatt tccagcgacc gttcagactc atttcgatgc
      181 gcatacacgg ttcctcgatt gatcaaacaa gaatggctgc aaaacgcgtc gatgattaca
      241 gcggcttgtg acacggtaca ggtttactgg aaacacggtt tcggggcaag cgtcggggac
      301 ttccacgtgc gttatgttcg gaggtgaaga aacgtgacga tgttgcgtcc gtagtttcgt
      361 tgatccaccg gttcgagtcc cgatgcgaag agaccgccgg cagtgtcctg ctcgtgatga
      421 tagacgatga gaccgtcggg tgcgagcagg cgttcggcca ctgatgcgat gagcctttcg
      481 agagcaatgc ggcgcggagg tttgccgaga gcgaacggcg gatcgaacag gacgatatcc
      541 gcgcgaagac cttccgccgc cagttgcgcc acggctgcgc gggagtctcg ttcgaggatg
      601 cggccgcggt cggcgagctt cgtgcggtcg agatttcgcg cgatggtgcg agcgcatgtg
      661 cggtcgcgat cgacgaacgt gcagaaccgt gcaccacgac tcagcgcctc gatgccgagt
      721 ccacccgttc ccgcgaacag gtcgagtacc tccgcgtcgc caagcaacgg atcgaggatg
      781 ctgaaaaccg acatttttac gcggtcggtc atggaacgcg tagtctgcgc cttcacctcg
      841 tagagcggca cgctgcccgc gattccgccg attacccgca cgatgccctc cgattctttc
      901 gtttcccacg attccttgcc gacaaacaat gatactcgta tcttcacaaa tgccccagtc
      961 ggtcccggta ttctcccctg aaaacgtgga atggtactgt gaccgacgca ccgtaacggc
     1021 gggctgtgcc ggattttagg cgcggtttcc gtgcggatgc gtcggacgac gtgggcacat
     1081 cggcgccgat ggaatttcgc gaatgtccgc aatcggcgaa cgtttttcat cagcggtctc
     1141 accaaacaag gcaaggatac ttatgggtag cacgttgttc tgggaagcgc aggagtttag
     1201 cggtacgatg caggtgcggt gccgtgacga actcatatcg ctcgcggaga ccgcgggttg
     1261 ggaatatgag gttgcgcagg atgtgtggga aggtgtcgtc gtcaacttga acgcccggcc
     1321 cgaacgtgcg cgtccgatga aggtccaact gcgtacgatc ggtgtgacga tgttcccgtt
     1381 caaccgtggc gagaacgacg accgcccgcg gctgtcattc gtcttcgaca acacgaacct
     1441 gaacgatcca gaccttcgga atcggctcat caccatagca ccgcccgcgt attggcatga
     1501 gtggatgccc cgtgttaaag gtcaatatcc agcgctaacc gatgaacgtg aagaaacgat
     1561 ctacggtctg cgcgccgacg gaatgatgcg tgttcgccgt accgagctac ccggcttcat
     1621 caaatttctc aatacccttc gcgcgacggc tttgccgctt ctcgatatcc gcacgagcga
     1681 ccgtctcgcg cggaaaatga tcaacgatcc gaacgcaaat cccttcgggc ggaatccaca
     1741 acctcgcccg ctcgatcagg ctacgcttga cgaaatcgcc gaaggactgg gcaacctgtt
     1801 cagcgcgcct cggtatccgg
//
LOCUS       DSCC01000407            2325 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1031325, whole genome shotgun sequence.
ACCESSION   DSCC01000407 DSCC01000000
VERSION     DSCC01000407.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1031325"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            272..964
                     /locus_tag="ENN56_05035"
     CDS             272..964
                     /locus_tag="ENN56_05035"
                     /inference="COORDINATES: protein motif:HMM:PF01872.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin deaminase"
                     /protein_id="HDS74890.1"
                     /translation="MSDTNTSEANVRCVMAMTADGKIASRDREAARFGGPADQRLVRE
                     QVAWADALLMAAGTLRAYGGTFEVCRPELMEMRRSTGQASQPTSVIVSESLDLPLDLH
                     FFTEQQIPRIITTTERQYDAARDRFAGVADVVARGSSAVDIAAVVDELRARGMRRILL
                     LGGGELNYACFRARIVDELYVTVSPLLYGGRNAPTLLGGDGFVAKDAVRLSLSEHEII
                     DGEVFLRYQVEY"
     gene            1003..1788
                     /locus_tag="ENN56_05040"
     CDS             1003..1788
                     /locus_tag="ENN56_05040"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74891.1"
                     /translation="MLSIATDYAADKGDPSPYLRRIADAGFTHVHWCHQWNTDFVYMD
                     AEIEQIGKWLNEYGLELLDLHGSVGPEKSWLSAREYERLAGVALVKNRIEMTARLGGD
                     AVVMHIPGEPGSEPLHRSLSELEPFARERGVRIAVENGVFAAISDVLSQYSPEYVGLC
                     YDSGHGNMSGEGLDRLAENTDRLCVLHLHDNDGKSDQHQVPFMGTIDWKRLAGIVAKS
                     AYSKPVQMEVSMKQSGFEDEIAFLDKAREVGVRIAELIESAKS"
BASE COUNT          469 a          633 c          715 g          508 t
ORIGIN      
        1 accgcgtcac tctcgctcgg cgtcacgctg gaccatgtcg tgagcatgtc gattcccata
       61 cttggcggtg cgacgtgggt gttgttcggc gtggaatacg tgttcgtcgg cgcggcggtg
      121 ttggcgctgt gcaacagtgt tatggcgacg ttcgtccgga ttcccgatca agcgacactg
      181 acgggaacgg tggcgccgaa cgtgggtgcg gcgtctgacg tgcgaacgaa gccggttacc
      241 gcgtaaacgc gacgcaggca ggtggtgccc tatgagcgac acgaacacca gcgaagcgaa
      301 tgttcgctgt gttatggcga tgaccgcgga cgggaagatt gcctcacgcg accgagaagc
      361 ggcgcgattc ggcggtccgg cggatcaacg gctggtgcgc gagcaggtcg cctgggccga
      421 tgcgttactc atggcggcgg ggacgttgcg ggcctacggc gggacgttcg aagtttgtcg
      481 acccgaactc atggagatgc gtcgatcaac tggacaagcg tcacagccga ccagcgtgat
      541 cgtcagtgag tctctcgatc tgccgttgga cctccacttc tttactgaac aacaaatacc
      601 tcgcatcata accacgacgg aacggcaata cgacgccgcg cgcgatcgat tcgcaggcgt
      661 cgcggacgtc gttgcgcgtg gatcgtcggc tgtggatatt gcggcggtgg tcgacgagct
      721 gcgcgcgcgt ggaatgcgcc ggatactcct gctcggtggt ggcgagctca attacgcatg
      781 tttccgggca cgaatcgtag acgagcttta cgtaacggtg tcaccgctcc tttacggcgg
      841 acgtaatgcc ccaaccttgc tcggcggcga tggattcgtc gcgaaagacg ccgtgcggtt
      901 gtcactcagc gagcacgaaa ttatcgatgg cgaggtattt cttcggtacc aagtcgaata
      961 ctgacgcgtt gtatttgttc tcatcgacgg aaaggattcc tcatgctgag catcgcgacc
     1021 gactacgcgg cggataaagg tgacccgtcg ccatacctcc ggaggatcgc cgacgcggga
     1081 ttcacccatg ttcactggtg tcatcagtgg aacaccgatt tcgtgtacat ggatgcagaa
     1141 atcgagcaga tcggcaaatg gctcaatgaa tacggtttgg agctgctcga tctccatgga
     1201 tcggtcggac ccgaaaagag ttggctctcc gctcgcgaat acgaacggct tgcgggtgtt
     1261 gcgcttgtga agaatcgtat cgagatgacc gcgcgactcg gaggcgacgc cgtggttatg
     1321 cacattcccg gtgaaccggg atccgagcca cttcatcgct cgctcagcga actggagcct
     1381 ttcgcacgag aacgtggcgt ccgaatcgct gtcgagaacg gtgtatttgc cgcgatatca
     1441 gacgtactct cgcagtattc gcctgaatac gtcggactct gctacgactc ggggcatgga
     1501 aacatgagcg gcgaaggtct cgatcgactt gctgagaata ccgatcggct ctgcgtgctc
     1561 catctccacg acaacgacgg aaagagtgat caacatcagg tgccgttcat gggcaccatc
     1621 gactggaagc gactcgcggg aatcgtcgcg aaatcggcct actcgaaacc ggtgcaaatg
     1681 gaagtttcca tgaaacaatc gggatttgag gacgaaatcg cgttcctcga caaggcgcgc
     1741 gaggttggag tgcgtattgc cgaactcatt gaatccgcga agagctgata cgtcatcgta
     1801 aacaaacggg gcgcgagatt cttctctcac gccccgtttt tcatttttcg tcgcgcggct
     1861 tcgttactcg gggagttttc cggagaccat ctctgccatg ccttctatct gcgctgatac
     1921 aacctttcgc gcaagttgcg gatcgtagtt atcgagtgtc ttccgattga agaagtcgaa
     1981 tccgatatcg agcaatgagt cgcggtagat gcggtagttg atgtcgttca tcttcgcggt
     2041 gacctgatcg cgcggaacgc caagatcgag tgccgtctga aacatcagtc cgtgaagcgc
     2101 ccggaggctc gaaaggtctt cgcgcgcggc gatgagtgga gttatcccgc ttcggcttct
     2161 gagcgcgttg tagatgcggt ctcgttcctt ttcgcgttcg tcggcaatcg cttcgctcgc
     2221 gatcgtctca agcacgtcga tccgctcacg gaggtccacg ttgagcatac cgagattgat
     2281 gccgtgttcg gcgaacacca cgagaatcgt ccggtctttc cgcag
//
LOCUS       DSCC01000408            2998 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1022377, whole genome shotgun sequence.
ACCESSION   DSCC01000408 DSCC01000000
VERSION     DSCC01000408.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1022377"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(123..959)
                     /locus_tag="ENN56_05045"
     CDS             complement(123..959)
                     /locus_tag="ENN56_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS74892.1"
                     /translation="MRFGGPIFGDVPGPDAWVAAHHRKGFRAAYCPLGADANDDTIRA
                     YEDAAHTADLTIAEVGAWSNPLSPDSTEREKALAHCRNQLELAERIGARCCVNISGSR
                     GAKWDGPHPANLTDETFDRIVETVRGIIDAVKPDRTYYCLETMPWAYPDSTDSYERLI
                     DAIDRRRFAVHFDPANLLCSPQRYYASGALIRDFVRRLGQYIKSCHAKDILLSDRLTT
                     HLDEVRAGLGGMDYGAFLESVATLDPDVCVMLEHLPNEAEYDAAAAHIRGVAKDLGVE
                     IS"
     gene            complement(1133..2395)
                     /locus_tag="ENN56_05050"
     CDS             complement(1133..2395)
                     /locus_tag="ENN56_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDS74893.1"
                     /translation="MSESNLFASVEKQLNEAADVMGLDSGIRAMLLTPMREMHFSIPI
                     KRDDGSCEMFHGFRVQYNWSRGPCKGGIRFHPKENIETVRALAAWMTWKTALMDLPLG
                     GAKGGVICDTHELSDRELEAISRGFIRRIYKYIGPDQDSLAPDMYTNPKIMAWMTDEY
                     ATLTGGQNFGVVTGKPLSIGGSRGRDAATAQGGIHTILEAAKRLKMDLNGVTVAIHGY
                     GNAGRYATEILNKDPRFRVVAVSDSKNGIYNADGIDGEAVTKAKQRTGTVQGLEGVKK
                     ITNDELLALDVDILICAAIENTITAENADSVKAKIVAELANGPTTPEADEILFGRGVH
                     IIPDFLCNAGGVTVSYLEMVQNEYRYAWDYSRVAEELERRMVRAYREVYDESQSHGVN
                     MRIAAYIVAMRRVITAMEDRGWVTPACT"
     gene            2613..>2998
                     /locus_tag="ENN56_05055"
     CDS             2613..>2998
                     /locus_tag="ENN56_05055"
                     /inference="COORDINATES: protein motif:HMM:PF03747.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74894.1"
                     /translation="MSTLLTRIHGCEAAGTIGNSMGDPVEGMYWTEIEKKFGFVDTLM
                     EQDKKDGRVQQPFGEDFVYHAHHRPPGTTEDGQERHRLCANAILRKGGRITIEDLAAT
                     WLCDIDPEKFGYLLGPQDRIIYLQLKA"
BASE COUNT          614 a          906 c          856 g          622 t
ORIGIN      
        1 gatcagtgtg gcgagtgtcg cgccacgaac ttcgcgcgac ccaagcggcg cgccgtcatc
       61 cgtacgcgtg acgcattccg cgcgacgtgg tcccgtaaag agcacctgac aatacgattc
      121 actcatgaga tttcgactcc gaggtctttc gcaacgccgc gtatatgagc cgccgccgca
      181 tcgtactcag cttcgttcgg caggtgctcg agcatcacgc acacatccgg atcgagggtc
      241 gcgacggatt cgagaaacgc gccgtaatcc atgccgccga gacccgcgcg aacctcgtca
      301 aggtgtgtcg tgagtcggtc ggagaggagg atgtccttcg cgtgacagct tttgatgtac
      361 tgaccgagtc ggcgtacgaa gtcgcgaatg agcgcgccgg atgcatagta ccgttgcggg
      421 ctgcacagca ggttcgccgg atcgaagtgg acggcaaatc gtcgccggtc gatcgcgtca
      481 atcagtcgct cgtacgaatc cgtcgagtcg ggatatgccc agggcatcgt ttcgaggcag
      541 taatacgtgc ggtccggctt gacggcgtcg atgataccgc gcaccgtctc gacgatacgg
      601 tcgaacgtct cgtctgtgag gttcgccgga tgcgggccat cccatttcgc tccgcgcgac
      661 ccggagatat tcacgcagca ccgcgcgccg atccgctccg caagttcaag ctgattccgg
      721 cagtgcgcaa gcgctttctc gcgctcggtg ctatcgggac tgagcggatt cgaccacgca
      781 ccgacttccg cgatcgttag gtcggcagta tgtgccgcat cctcgtacgc gcgaatcgtg
      841 tcgtcgttcg catcggctcc gagcggacaa tacgccgcgc gaaagccttt tcgatgatgt
      901 gccgccaccc acgcatcagg accgggaacg tccccgaata tcggaccacc aaatcgcatg
      961 gaatcatcct cctcatacga ttgaaatgga gtaaaacact tttgagtata cacgaaatcc
     1021 ggttagtggg aacggcagcg acagcattca tgcgaatcgc aaacaaaaac gccgcgcacg
     1081 aggtcatacc catcgtacgc ggcgaattcc gtgtgttttg gacaggaatc gattatgtac
     1141 aggcgggggt cacccagccg cgatcttcca tggcggtgat cacgcggcgc atggcgacga
     1201 tgtacgccgc gatacgcatg ttcacgccat gcgactgtga ttcgtcgtag acttcgcggt
     1261 aagcgcgaac catgcggcgc tcgagctctt cggcgacacg cgagtagtcc cacgcatacc
     1321 gatactcgtt ttgcaccatt tcaaggtacg aaacggtcac gccacccgcg ttgcacagga
     1381 agtccggaat aatgtgtaca ccgcgaccga agaggatttc gtcggcttcc ggcgtggttg
     1441 gaccgtttgc caactcggcg acgatcttcg ctttgacgct atccgcgttc tcggcggtaa
     1501 tcgtgttctc gatcgccgcg caaatgagga tatctacgtc gagtgcgaga agctcgtcgt
     1561 tcgtgatctt cttgacgcct tcgagacctt gtacggtacc ggtgcgctgt ttcgctttgg
     1621 tcaccgcttc gccatcgatg ccgtcggcgt tgtagatgcc gtttttcgag tcgcttaccg
     1681 caacgacacg gaagcgcggg tccttgttga gaatctccgt cgcataccga ccggcgtttc
     1741 catacccgtg aatggcgacc gtcacgccgt taagatccat cttgagccgc ttcgccgctt
     1801 caagaatcgt atggattccg ccctgagccg tcgccgcgtc acgtccgcgc gaaccaccga
     1861 tgctgagtgg cttgcccgtg acgacgccga agttctgtcc accggtgagc gtcgcgtatt
     1921 cgtcggtcat ccacgccatg atcttcggat tcgtgtacat gtccggcgcg aggctgtcct
     1981 gatccgggcc gatgtacttg tagatgcgcc gaatgaatcc gcgactgatc gcttccaact
     2041 cacggtcgga gagctcgtgc gtgtcgcaaa tgacaccgcc cttagcaccg ccgaggggca
     2101 aatccatgag cgccgtcttc cacgtcatcc acgccgcaag cgcgcgcacc gtctcgatgt
     2161 tttctttcgg atggaaccgg atgccaccct tacatggccc gcgcgaccaa ttatactgca
     2221 cgcggaaacc gtgaaacatt tcgcacgaac catcgtcgcg tttgatcggg atcgaaaagt
     2281 gcatttcgcg catcggcgtg agcagcatcg cgcgaatacc ggagtcgaga cccatgacgt
     2341 ccgctgcttc attgagctgt ttttcgacac tggcgaagag attactttcg ctcatcgaat
     2401 gtgtttcctc cacatatgaa tcgttgggcc gatcagggga tactcgtatg atcagcgtag
     2461 tgcaacgcct gctcacgcaa cctcgacgtt ggtactcaag gagagaacgc gattgccgac
     2521 gcacttgatg ccaaccttat aatatcgtaa aaaaccgtct cattgcacac ggggttcggc
     2581 gaacgttttt cgcacgccgt aaggagtcga ccatgagcac gttactcacg cgcattcacg
     2641 ggtgtgaagc cgcgggaacc atcggaaact ccatgggcga tccggtcgag ggcatgtact
     2701 ggaccgagat cgagaagaag ttcggcttcg tcgatacact gatggaacaa gacaagaagg
     2761 acggccgtgt gcagcagccg ttcggcgagg atttcgtcta ccacgcgcac catcgaccgc
     2821 cgggcaccac cgaagacggt caggagcggc accgactctg cgccaacgcg atcctccgca
     2881 agggtggccg cattaccatc gaagaccttg ccgcgacgtg gctctgcgac atcgatccgg
     2941 agaagttcgg ctatctcctc ggcccacagg accgcatcat ctacctccag ttgaaggc
//
LOCUS       DSCC01000409            1707 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1047748, whole genome shotgun sequence.
ACCESSION   DSCC01000409 DSCC01000000
VERSION     DSCC01000409.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1047748"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(627..>1707)
                     /gene="mgtE"
                     /locus_tag="ENN56_05060"
     CDS             complement(627..>1707)
                     /gene="mgtE"
                     /locus_tag="ENN56_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDS74895.1"
                     /translation="PDERADMFAEVEDDEERNKLLELLSPRARADIHRLLSYPPESAG
                     GIMTTAYVSVPETSTVDEATRLVRQVAQRAETIYTVYIVDGEGVLVGVIGLRTLLLAA
                     PDASVRDVMETAVISAEDSEDQEEVAWRLRNYDLTAIPVVDTEKRLVGIVTVDDVLDV
                     IREENTEDTYKMAAINEYETPYLDTPAWKLARQRAPWLMVLVLAGFLSGYVLERYESL
                     LDQLVVLIFFVPVLTGSGGNAGIQVSTTVIRSMATHELGPGDVWAVLRKEIGVALMIG
                     VAMGILAMGRAMFVDFHWRLFGTVGLAMAGVVGAAVIIGGLLPLGLKRIGLDPALMSS
                     PFITTVTDSLALIIYFEIARLLLTV"
BASE COUNT          371 a          533 c          512 g          291 t
ORIGIN      
        1 gaatcgctct acgcgatcgg accgtgcggc gatatcaacc atatcgacgt gaacgcggag
       61 aatcgacttg gcagacacga acacgcacaa acgatggcgc acacactcgt tgacgcagcg
      121 ctggccgtat gggaggaacg cgaggtaatc gacggcgcgc tcgacgtgcg ccggaccacg
      181 ttcacggtgt cgcgccgtga agtaaccgac gagcaaatcg ccgaggcgaa agcactcatc
      241 gcgcgcgaga tgaagcccgg tgacgagccg agcaccgagg tcgttttcgc acggggaatg
      301 ctgctgctct ccgagtcgcc attggaaatc gagatcgaga tctgtgccgt gataatcggt
      361 gagatagcaa tcgtctgttt gccgggagag ctgttcagcg agctcgcgga gaagatcgaa
      421 gacgcgtcac catatgctca cacgttctgc gtggagctgc tcgcaagcgc ggaagcggga
      481 tacatcggca cgtgggaagc atacgcggaa ggcgggtacg aaacgcgccc gatgccgtct
      541 tcgcagttcg ccccgggaac cggcgagcgc gtcgttgaag aggcggtgcg acttctcaca
      601 cagaagcgat agcaccctga cagctatcac acggtcagga gtagtcgcgc gatctcgaag
      661 tagataatga gcgcgagcga gtcggttacc gttgtaatga acgggcttga catcaacgcc
      721 gggtcgagcc cgatgcgctt tagccccagc ggaagcaatc cgccgatgat gaccgctgcg
      781 ccgacgacgc cggccatcgc gagaccgacc gtgccgaata atcgccagtg gaagtcgacg
      841 aacatcgcgc gacccatggc gagaatcccc atcgccacac cgatcatcaa tgcgacgccg
      901 atctccttcc gcagcaccgc ccaaacatcg ccggggccca gctcgtgggt cgccatggag
      961 cggatcaccg tcgtcgatac ctgaataccc gcattcccgc cggagccggt cagtaccggc
     1021 acgaagaaga tgagcacgac gagttgatcg aggagcgact cgtaccgctc gaggacgtat
     1081 cccgagagaa acccggccaa gacgagcacc atgagccacg gcgcacgctg ccgggcgagt
     1141 ttccatgccg gcgtatcgag atacggcgtt tcgtactcgt tgatagccgc catcttgtag
     1201 gtgtcttccg tgttctcctc gcggataacg tccaggacgt cgtcaaccgt tacgatacca
     1261 acgagccgct tttccgtatc cacaaccgga atcgccgtga ggtcgtaatt cctcagccgc
     1321 cacgcaacct cttcctgatc ctccgaatcc tcggcgctga tcaccgcagt ctccatcacg
     1381 tcgcgaacgg acgcatccgg cgcggcaaga agaagagttc gcaacccgat cacaccgacc
     1441 agaacgcctt cgccatcgac aatatagacc gtgtagatcg tttcggcgcg ttgcgcgacc
     1501 tgccgtacga gccgtgtcgc ttcatcgacc gtagaggtct ccggaaccga cacgtacgcg
     1561 gtggtcatga taccaccggc gctctcgggc ggataactca acaggcggtg gatgtcggcg
     1621 cgcgcgcgcg ggctgagcag ctcgaggagc ttgttgcgtt cctcgtcgtc ttcaacctcg
     1681 gcaaacatgt ccgcgcgttc atccggc
//
LOCUS       DSCC01000410            1595 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052381, whole genome shotgun sequence.
ACCESSION   DSCC01000410 DSCC01000000
VERSION     DSCC01000410.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052381"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..118)
                     /locus_tag="ENN56_05065"
     CDS             complement(<1..118)
                     /locus_tag="ENN56_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011610684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HDS74896.1"
                     /translation="MRWFFRLRVRFRVRGDSMRPLIADGDEVLVDTSAYRHST"
     gene            complement(163..636)
                     /gene="sodN"
                     /locus_tag="ENN56_05070"
     CDS             complement(163..636)
                     /gene="sodN"
                     /locus_tag="ENN56_05070"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HDS74897.1"
                     /translation="MGKKLIDAYRSTVPAPEVSAHCDGPCGVYDPASARIAAEAVVSM
                     TKKLKALTAPAAGDHAGWLAFHNTTTRYILIKEEQAHIAKKEILVLWTDYFKPEHLQK
                     FPDLHDKVWKATKLCSTCKHEINLDAAEKLLAAIKEIHEIFWATKNRDVAWYVAS"
     gene            952..>1595
                     /locus_tag="ENN56_05075"
     CDS             952..>1595
                     /locus_tag="ENN56_05075"
                     /inference="COORDINATES: protein motif:HMM:PF14326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HDS74898.1"
                     /translation="MGRRALNLSKTLLRGFALLAVVGVLIGCAGSLFQREAPMTMVWY
                     GLVRTGPFQEPRYSPLEDDVTLATGDGIQLFLTVEPDAYFYALHQTTTGQFAVLWPTE
                     EGGFSAFMESGRVQTLPSRGRVYTMEFGQGASALYFIASRKPIDSVAELLREMRTLFT
                     TAQAIAVGIPADQIKRNIPNSVEWKLDLKNPPPIELTDQHIAVAATVRSIGRIRT"
BASE COUNT          337 a          436 c          472 g          350 t
ORIGIN      
        1 gggtgctgtg acgatacgcg ctcgtatcga caaggacttc gtcgccatcg gcgatcaacg
       61 gtcgcatcga gtcgccgcga acgcggaatc gtacccgtag gcggaaaaac cagcgcacga
      121 gctcggcgaa cccgggcgct ggcacgtcat cgtttgtcgg cctcaactgg cgacatacca
      181 cgcaacgtca cgattcttcg tcgcccagaa aatctcgtgg atttccttga tcgccgcgag
      241 cagtttttcg gctgcatcga ggttgatctc gtgcttgcag gtcgaacaca gcttcgttgc
      301 cttccagacc ttgtcatgca ggtcgggaaa cttctgtaga tgctcgggct tgaagtagtc
      361 ggtccacaaa acgaggattt cttttttcgc gatgtgcgcc tgctcctctt tgatgaggat
      421 gtagcgcgtt gtcgtgttgt gaaacgcaag ccacccggca tgatcgccgg cggcgggagc
      481 cgtgagcgcc ttgagctttt tcgtcatgga aacaaccgct tcggcggcga ttcgcgccga
      541 ggccggatcg tatacaccgc agggaccgtc gcagtgtgcc gatacttccg gcgcaggtac
      601 cgtcgagcga tacgcgtcga tgagcttctt gccaatcatg gtgcaccatc cctttcgaaa
      661 gacgttgcga aaccgaacgc gttactgtga agagctccgg tcgttcgcgg tctgaactcg
      721 cgagctgcta tcgaaatagt agagaaacag gggccaagtg gcaataaccg accggtacca
      781 cgagtgttta ttacgtgtta cgccaagaag catcacagac gtacaggagg cggcggccac
      841 gcagagagaa tcatgcgtcc gcttgactat tggtcggttc acgcacaaac ttagttgcga
      901 tgcgtcagtc ggttcgtcga gagagatgaa tgagaccgtg catcaattac tatggggagg
      961 cgcgcgctga atctgagtaa aacgctgctg cgtggattcg cattacttgc ggtcgtcggc
     1021 gtgcttattg gatgtgccgg ttcgctgttc caacgtgaag cgccgatgac gatggtttgg
     1081 tacggactcg ttcggaccgg accgtttcag gagccgcgat attcaccact tgaagacgac
     1141 gttacccttg ccactggcga cgggatccag ctgttcctga ccgttgagcc agatgcgtat
     1201 ttctacgccc tacaccaaac gacgactggg cagttcgccg ttctttggcc aaccgaggaa
     1261 ggcggtttca gcgcgtttat ggaaagcgga cgcgttcaga cgcttccgtc gcgcgggcgg
     1321 gtatacacca tggagtttgg gcagggagcc tccgcactct acttcattgc aagtcgaaag
     1381 ccgattgatt cagtcgccga actgctccgc gaaatgcgta cgctcttcac gacggcgcag
     1441 gcgatcgctg tgggaatacc cgccgaccag atcaagcgaa acattccgaa tagtgtcgaa
     1501 tggaagcttg acctcaagaa tccgccgccg atcgagttga ctgatcagca tatcgcggta
     1561 gctgcgacgg tgagatccat cggtcggatt cgaac
//
LOCUS       DSCC01000411            2687 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1025872, whole genome shotgun sequence.
ACCESSION   DSCC01000411 DSCC01000000
VERSION     DSCC01000411.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1025872"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..805)
                     /locus_tag="ENN56_05080"
     CDS             complement(<1..805)
                     /locus_tag="ENN56_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74899.1"
                     /translation="MKALSLVYTATAACLLFVGGTASAVGMGYGQAAATGMAGAYTAS
                     ATGIHAANWNPANLAAFRYRSPGVEIGILNTGFKIGNDGIPWGDFVDWTADDVLTTEE
                     IQKALGQLSGNSLAVHQSAEFGMPFSMSIGRYAVSFGIVQMADLGISRGFLDMIADDP
                     SPDFTDEQAIIDRYEESKAGNIIRDLSGMKADFWALATFGVSHARLIDVDPFDRFAVG
                     GTINYYAASPRMRIVESSGQAVVYPNGWDMDSQLVMELAGASLTRTTDAW"
     gene            complement(819..2669)
                     /locus_tag="ENN56_05085"
     CDS             complement(819..2669)
                     /locus_tag="ENN56_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74900.1"
                     /translation="MTFSSSDDPTLNNVVISFSNHTPLTMGPVEIFLSNTGDTTATGD
                     VTDYYAYTVIAAVAPGTTESSDPIVLSNATVNSPTYVITRTAFQTGTNINSDEIRNGY
                     FVTAVDISELEVIEAVAQIPAQEFSKSQSRSLTHSQLELIEVELAEVSDPDTNRFQMS
                     ITNNIATPVNILFDIPDFTSIPYAPYTSGSGVLIPENPNDWSRATITLGANESIDIVY
                     NLDGAIIKNSAVDRAPIGDLEIDLNIEILGTGNDFVHIQSTDAATVVTNMSALAIQRV
                     TGLVPSTNPITSPINEFDFVVDESMPEGIAGIQARAIRAALDIATHDIRGVATIRLIM
                     TVDQRYPDDLTGYYERVIHAEIEPGTVVPFVATEDSLGELGNSPLAIINAMLSNVFNN
                     GYGRMLVNGEVAFEDTVVLVRDVSRIEIRDMNIETPLMFDVPVITFDARSSDQDGFDL
                     DLSDGVREDMIPRAKGMALVAHVRNHFPLGGRLTLYISPDPRFLELRNAYPASAQTPL
                     NQIPTVLPTDVNIANLDEVRYRKAVYSLFTIELPEPQRLADGTADDANPGETTVLITL
                     SEPVIQLFSLENSYILPRIQLLEEPGLVKLEADDYLEIDLWAQLKATTKK"
BASE COUNT          609 a          763 c          713 g          602 t
ORIGIN      
        1 cccacgcatc ggtcgtacgc gtaaggctcg caccggcgag ctccattaca agctgcgagt
       61 ccatatccca accgttggga tagacgaccg cttgacctga tgattccaca atgcgcattc
      121 gaggactcgc ggcgtagtag ttgatcgtgc caccgaccgc gaacctgtcg aacggatcga
      181 cgtcgatcaa ccgtgcgtgc gagactccga atgttgccaa tgcccagaaa tcggccttca
      241 tacccgacag atcacggatg atattccccg ccttcgattc ttcataccga tcgataatcg
      301 cttgttcgtc ggtaaaatca ggcgacgggt cgtcagcgat catgtcgaga aaaccgcgtg
      361 aaatgccaag atcggccatc tgcacaatgc caaacgacac cgcatagcgg ccgatcgaca
      421 tcgaaaacgg catgccgaat tcagcgctct ggtgaaccgc gagagaattc ccggaaagct
      481 gaccgagcgc cttctggatt tcctccgttg tgagcacatc atcggcagtc cagtcgacga
      541 aatcgcccca cgggatgcca tcgttcccga tcttgaaacc ggtattcagg atgccaatct
      601 caacgcccgg actgcggtac cggaatgcgg cgaggttcgc cgggttccag ttcgccgcat
      661 gaattcccgt tgctgatgcc gtatatgcgc cggccatacc agtcgccgcc gcttgaccat
      721 aacccatacc gacagccgaa gccgtgcctc ccacgaagag caggcaggcg gccgtcgcag
      781 tgtaaacgag cgatagtgcc ttcatcgttg tctcctccct acttcttcgt tgtggctttg
      841 agttgcgccc aaaggtcgat ttcgaggtaa tcatccgcct ccagcttaac cagacccggt
      901 tcttcgagga gctgtattcg cggaagtata tacgagttct ccaacgagaa cagctgaatc
      961 accggctcgg aaagcgtaat gagcaccgtc gtctcaccgg ggttcgcgtc atccgctgta
     1021 ccgtcggcaa gtcgttgcgg ttccggcaac tcaatcgtga acaagctgta taccgccttt
     1081 cgataacgta cttcatcgag attggcgatg tttacgtccg ttggaagaac tgtcggaatc
     1141 tgattcaacg gcgtctgtgc actggccgga tacgcgttcc gtagctccaa gaaacgcggg
     1201 tcaggactga tatacaacgt gagccgtcca ccaagtggga agtgatttcg aacgtgcgca
     1261 acgagcgcca tcccctttgc gcgtgggatc atatcttctc gtacaccatc actaagatca
     1321 aggtcaaacc cgtcttgatc tgacgaacgc gcatcgaacg taatcaccgg cacgtcgaac
     1381 atgagcggtg tctcgatgtt catgtccctg atttcgatgc ggctcacatc tcgaaccaga
     1441 acaacggtgt cttcgaacgc cacctcaccg ttcaccaaca tgcggccgta gccattgttg
     1501 aagacattcg agagcatcgc gttgattatg gcgagcggcg aattacccaa ctcaccgagg
     1561 ctgtcttctg tcgcgacaaa aggaaccacc gttccgggtt caatttctgc gtgaatcacg
     1621 cgttcgtaat accctgtcag gtcgtcgggg taccgctgat cgaccgtcat aatgagccga
     1681 atcgtcgcaa cgccgcggat atcatgggtg gcgatatcga gcgctgcacg aatcgctcga
     1741 gcctgaatcc ctgcgatacc ttccggcata ctctcgtcga cgacaaaatc gaactcgttg
     1801 atcggcgagg taatcgggtt cgtcgacggc acaaggccgg tcaccctttg gatggcgagc
     1861 gcactcatgt tcgtaacaac ggtagccgca tcggtcgact gaatgtgcac gaagtcattt
     1921 ccagtaccaa gaatctcgat attgagatcg atctcgaggt caccgatggg tgcacgatcg
     1981 actgcgctgt tcttgatgat cgcaccgtcg agattataca cgatgtcaat cgactcattc
     2041 gcgccgagtg taatggtagc acgtgaccag tcattcgggt tttcgggaat aagcacacct
     2101 gagccgctgg tatacggtgc gtatggtatc gacgtaaaat ccggaatatc aaaaaggata
     2161 ttgaccggcg tcgcgatgtt gttcgtaatc gacatctgga aacggttcgt atcgggatcg
     2221 ctgacttccg cgagctcgac ttcgatcagc tcgagttgac tatgcgtgag cgatcggctc
     2281 tgcgacttgc tgaactcctg cgcgggaatc tgcgcgaccg cctcgatcac ctcgagctcc
     2341 gaaatgtcta ctgccgtaac gaagtacccg tttcgaattt cgtccgagtt gatgttcgtt
     2401 ccggtttgaa acgccgtacg cgtaatgaca tacgttggac tgttcactgt cgcgttgctt
     2461 aaaacgatag gatcagacga ctctgtcgtg ccgggcgcaa cggcggcaat caccgtgtac
     2521 gcgtagtaat cggtgacatc acccgtcgca gtcgtatctc cggtgttcga aaggaagatc
     2581 tcgactggtc ccatcgtgag cggcgtgtgg ttcgagaatg agatcacgac attattcagc
     2641 gtcggatcgt ccgaactcga gaacgtcacc gtggtaaact tgtcgaa
//
LOCUS       DSCC01000412            4011 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1015076, whole genome shotgun sequence.
ACCESSION   DSCC01000412 DSCC01000000
VERSION     DSCC01000412.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1015076"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..308)
                     /locus_tag="ENN56_05090"
     CDS             complement(<1..308)
                     /locus_tag="ENN56_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDS74901.1"
                     /translation="MDAGSLFSNVPTALLDEFTEILVSGQDVTVERIVSHGHRSPDGF
                     WYDQDRSEWVMVVSGNAKIEFADGAIRDMNPGDWVTISPHEKHRVSWTNPECDTVWLA
                     V"
     gene            complement(323..1090)
                     /locus_tag="ENN56_05095"
     CDS             complement(323..1090)
                     /locus_tag="ENN56_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS74902.1"
                     /translation="MNSLDLFRLDGKVALVTGASRGLGVEMAQALVDAGADLAISSRN
                     EENIRSIAKSLSTEERVVLPLVADVTKPDEVKAMVEAVIEKFGRLDILVNNAGVNIRR
                     PFEEYDVSEWKTIIDTNVIGVLACSQAVLPQMRKQKWGRIINIGSMMGTVGMPTRVPY
                     AASKGAVHQISRTLCHEVAKDGININTIAPGPFMTEMNKAVIDQPDVYNFFINRVPLG
                     RWADPKELRGITLFLASEASSYVTGTTIHVDGGWTAA"
     gene            1468..1935
                     /locus_tag="ENN56_05100"
     CDS             1468..1935
                     /locus_tag="ENN56_05100"
                     /inference="COORDINATES: protein motif:HMM:PF00571.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDS74903.1"
                     /translation="MPFQETLEETQIRHLNLPSPNVLSPTISIRSAIKHMRKVKCACG
                     LVVKNEKLIGIVTERDVMNKVVGRPEMVGRQIREIMTPNPVTLGPDNSLDDAIELMSR
                     GGYRNVPIIDDENRAIASLNVHQILQFILENFPQDANNLPPRPEQQIPTPEGA"
     gene            2000..3829
                     /locus_tag="ENN56_05105"
     CDS             2000..3829
                     /locus_tag="ENN56_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDS74904.1"
                     /translation="MTPNVRELDTVTIRFAGDSGDGMQMIGAQFTTASALAGNDISTL
                     PDFPAEIRAPAGSLPGVSGFQISFSSHDILTPGDEPDVLVVMNPAALKVNLSDLETGG
                     MVILNTDEFTKANLQKAGYAKNPLEDGTLSGYQVVSIPITTLTLETLTDSPLSAKNKE
                     RCKNTFALGVLYRVYDRPTAQTIEYYNRYFGRKDPAIAEANVAVLRAGYNFADTTQVF
                     QTFYKVRKAQLRTGKYRKITGNEATAIGLITASVRAKRPLFYGSYPITPASDVLHELA
                     AKKHFGVITFQAEDEIAAMGSTIGAAFAGAIGVTGTSGPGLALKSEAINLAVMTELPV
                     VIANVQRGGPSTGLPTKTEQSDLLQAMFGRNGDSPVPVIAPCTPSDCFWMAIEAVRIA
                     VKYMTPVLFLSDGYLANGAEPWRIPEIDELPEIPVKFAHDPESYMPYLRDTETLARPW
                     AIPGTPGLEHRIGGLEKADVTGNVSYDPANHEKMTKLRAEKVARIADDIPELTVHGDA
                     EGDLLVLGWGSTYGAITSAVDDLRKEGIAASSAHLQYLNPFPRNLGDVLKRFKRVMIP
                     EMNMGQLAFLVRSRYLIDVISHTKIKGRPFTIHEIIDAVKKSL"
BASE COUNT          986 a         1035 c         1082 g          908 t
ORIGIN      
        1 acggcgagcc atacggtatc acattccgga ttcgtccatg agacacgatg tttttcgtgg
       61 ggtgatatcg tgacccaatc acccggattc atgtcgcgaa tcgcgccgtc tgcaaactcg
      121 atcttcgcat taccggatac gaccatcacc cattcggacc gatcctgatc ataccagaat
      181 ccatcgggcg accggtgacc gtgcgaaacg attcgttcca cggtgacgtc ctgaccggat
      241 acgagtattt ccgtgaactc gtccaggaga gcagttggaa cattcgagaa gagactgcca
      301 gcatccatcg atttgcgttc gcttaggctg cggtccagcc gccgtcgaca tggattgtcg
      361 tgccggtgac atagcttgac gcttccgatg cgagaaataa cgtaatccca cgcaactcct
      421 tcggatcagc ccaacgacct aacggtacgc gattgatgaa gaagttgtag acgtccggct
      481 ggtcgatgac cgctttgttc atctcggtca taaacggtcc cggggcgatt gtgttgatat
      541 tgatgccgtc ctttgcaacc tcgtgacaca gcgttcgtga gatttggtgc actgcgcctt
      601 tcgatgcggc gtagggaaca cgagtcggca tgccgacggt acccatcatc gaaccgatgt
      661 tgatgattcg accccatttt tgtttccgca tttgaggtag tactgcttgc gaacacgcga
      721 gtacaccgat tacgttcgtg tcaataatcg ttttccattc ggaaacgtcg tattcctcga
      781 acggacgacg gatattcacg ccggcattgt tcacaaggat atcgagacgg ccgaatttct
      841 cgatgaccgc ctcgaccatc gccttcacct cgtcaggttt cgttacatcc gcgacgagcg
      901 gaagaaccac gcgttcctca gtactcaacg atttcgctat cgaacggata ttctcttcgt
      961 ttcgactcga aatcgcgaga tccgcgccgg cgtccacaag tgcttgcgcc atttcaacgc
     1021 caagtccacg gcttgcaccg gttaccaatg ccacttttcc gtcaagtcgg aagagatcaa
     1081 gcgaattcat acatgcgctc cttgctgtta cgatatcggt gatcgatacg gtcgaagaga
     1141 tgatacggtc cgttcggcgg tggctcaacg gctcatgttt tacgagtgta tggacaaggt
     1201 gacatgttga gatgtagatt tggtgcgtat tcgcgagtct gtcggcgccg agcgaagcga
     1261 tccttttgaa gaggtgctcc ttcttttata ttggctatga attacgaatt gatacccgac
     1321 agtgaattgt cgtgattctc gatatatcag aaaccacgcg atcgattcgt tgtggtgtat
     1381 ctttacgact gtcggataat gactaatcgt ccattcatcc ggctgattgg gaatgggtaa
     1441 catcgtcttt gattggggag gccgggtatg ccgtttcaag agacgttgga agagacgcag
     1501 atcaggcatc tcaatctgcc ttcgccaaac gtgctctcgc cgacgatctc gattcgttcg
     1561 gcgatcaagc atatgcgaaa agtgaaatgt gcgtgcggac tcgtcgtcaa gaatgagaag
     1621 cttattggga ttgtaaccga acgcgacgtt atgaataagg ttgtcggtcg ccccgaaatg
     1681 gtcggcaggc agattcgcga gatcatgact ccgaatcccg taacgctcgg tcccgacaac
     1741 tctctggatg acgcgatcga gttgatgagc cgtggcgggt accggaacgt tccgataatc
     1801 gacgatgaaa atcgagcgat cgcttcgctg aacgtccatc agatactcca attcatcctc
     1861 gaaaactttc cacaagatgc aaataacctt ccacctcgac cggaacagca gattcctacg
     1921 cctgagggcg cgtaacgcgc gaacgatgag gagaggaccg atactgatgg cagttgaaac
     1981 gactgtaccg ggtaacgaaa tgacaccgaa cgttcgagag ctcgacacgg tcactattcg
     2041 ctttgccggt gattccggcg acggaatgca gatgatcggc gcgcagttca ccaccgcctc
     2101 cgcgcttgcc gggaacgata tcagcacgtt accggatttc ccagcggaga tacgagcacc
     2161 tgcgggatca ctgcccggcg tcagcggatt ccagataagc ttcagtagcc acgatatctt
     2221 gactcccggt gacgaacccg acgtgctcgt cgtgatgaac cccgcggcgt tgaaggtgaa
     2281 tctttcggat ctcgaaaccg gtggaatggt tattctgaac acggacgagt ttacgaaggc
     2341 gaatctccaa aaagcggggt acgcaaagaa tccgctcgag gacggaacgc tttcgggata
     2401 tcaggtcgtt tcgataccga ttacaacgtt gacactggaa acacttacgg atagcccact
     2461 ctcggcaaag aacaaggagc gatgcaagaa cacgttcgcg cttggtgtcc tttaccgagt
     2521 gtacgaccgc ccgaccgcgc agactatcga gtactacaat cggtatttcg gtaggaagga
     2581 tcccgcgatc gcggaagcga atgttgccgt tcttcgcgcg gggtataatt tcgctgatac
     2641 tactcaggta tttcagacgt tctataaggt tcggaaagca cagctgcgta ccggcaagta
     2701 ccgcaagata acgggcaatg aagctacggc gataggtctc attaccgcct cggtgcgagc
     2761 aaaacgccca ctcttctatg gaagttaccc gattacgccg gcgagcgatg ttcttcacga
     2821 actcgctgca aaaaaacact tcggtgtaat cacgtttcaa gctgaagacg aaatcgccgc
     2881 gatgggttcg acaatcggtg cagcgtttgc cggcgcgatc ggcgtgacag ggacgtcggg
     2941 accaggcctc gcactgaaaa gcgaagcgat taacctcgct gtcatgacag aactgccggt
     3001 ggtaattgcg aacgtccaac gcggcggtcc cagtactgga ctaccgacga agaccgaaca
     3061 atctgacttg ttacaggcga tgttcggacg gaatggagat tcgcctgtgc cggttattgc
     3121 gccatgcacg ccgagcgact gtttctggat ggcgattgaa gccgtgcgaa tcgccgtaaa
     3181 atacatgacg ccggtattat tcctctccga tggttatctt gccaatggcg ccgagccgtg
     3241 gcgcattccc gaaatcgacg agcttccgga gataccggtg aaattcgcac acgatcccga
     3301 gtcgtatatg ccatatcttc gcgacacgga aacgttggcg cgtccgtggg cgatcccggg
     3361 tacgccgggg ctcgaacacc gtatcggtgg cctcgaaaag gcagacgtga caggaaacgt
     3421 aagctatgat ccggcaaatc atgagaaaat gacgaagttg cgtgcagaaa aggttgcccg
     3481 aatcgcggac gacataccgg aactcaccgt acatggtgat gcagaaggtg accttttggt
     3541 tcttgggtgg ggaagcacgt atggcgcgat tacgtcagca gtagacgatc tacgaaaaga
     3601 gggtatcgca gcgtcaagcg cgcatttaca gtatttgaac cccttcccga gaaatctcgg
     3661 agacgtactc aagcgattca aaagggtaat gattccggaa atgaacatgg gtcaactcgc
     3721 gtttcttgtt cgaagtcgat atctcataga tgtgatctcc cacacgaaga taaaaggacg
     3781 accgtttacg attcatgaga ttatcgacgc cgtgaagaaa tcactctgag gttacgagcc
     3841 atgaacgagc atgaaaccgt cgagaacaac gttgacaatt caacgaacga agaaacgaaa
     3901 actgaaaatc gtccatcctc atcgagggaa ttgccacaac tttctcggaa ggacttcgtg
     3961 tccgatcagg aagtgcgctg gtgtccggga tgtggtgatt acgcaatact c
//
LOCUS       DSCC01000413            4258 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1013946, whole genome shotgun sequence.
ACCESSION   DSCC01000413 DSCC01000000
VERSION     DSCC01000413.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1013946"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            239..775
                     /locus_tag="ENN56_05110"
     CDS             239..775
                     /locus_tag="ENN56_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74905.1"
                     /translation="MKHTFGILAVTALVIAGIAAASDNSITGNTPTSTMAPSYRPTIE
                     SAPTVDLMGLLQRQGIFDQNRVSTWRTYSFGMAMGGGQTTSGGLLVQHMQYQLSKPLT
                     LHVALGLEHNPLGMAGMNIGGPQQASLTIPSVDLIYRPSDSFIFSFHYSQVPSYGYDS
                     GTWWEREAMSPYSYGSRW"
     gene            751..1212
                     /locus_tag="ENN56_05115"
     CDS             751..1212
                     /locus_tag="ENN56_05115"
                     /inference="COORDINATES: protein motif:HMM:PF13399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HDS74906.1"
                     /translation="MFIRFAVVTTSTETHSRFRIVYRLVAAFLIALVVAACTSSERDE
                     PSVDTVSHAPIVVVFNGCGAPGAARAIAEALRAEGWDIGNGYGENAESFDYPDTFVVD
                     YVGDRETAQRIAEKLGTFMIQQISHDPDRFGTVGVIVGADYRARVAQLLEG"
     gene            1213..1599
                     /gene="rsfS"
                     /locus_tag="ENN56_05120"
     CDS             1213..1599
                     /gene="rsfS"
                     /locus_tag="ENN56_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDS74907.1"
                     /translation="MVQTAQAEKQADRTISDDVARLVEAARSKKARDIAVLDLRGSSD
                     VTDFFVICTADVDSHARAIADGIVESQRIATGRKPWHIEGTDKMNWVLIDYVDVVVHI
                     MRDDTRKFYGLEEIWGDASRTDYPNE"
     gene            1818..2741
                     /locus_tag="ENN56_05125"
     CDS             1818..2741
                     /locus_tag="ENN56_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDS74908.1"
                     /translation="MSDSLLIVGSVAYDSVETPTGIRVDALGGSATFAAAAASLFTPV
                     DLVGIVGDDFDLAGLSFVTDRGVDLTGLEIRKGGKTFRWSGVYETDVNIRHTRSTDLN
                     VFADFNPTLPAKYRSDDVVFLGNMLPSLQQRVLEQTTNPIWVALDTMDLWINTTREEL
                     LDVMRKVQMVIINDSEARLLTGESNLVRAAQNVLTLGPTAVVVKKGEHGVFLVMKEGF
                     AVLPALPLENVVDPTGAGDSFAGGMLGALNATRDFSLDGLKRAMAYGTATASLTCEAF
                     SIDALKTANRDELNYRYEKLRGIASLPPLSV"
     gene            2764..3816
                     /gene="mtnA"
                     /locus_tag="ENN56_05130"
     CDS             2764..3816
                     /gene="mtnA"
                     /locus_tag="ENN56_05130"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HDS74909.1"
                     /translation="MPTETLKWIDGEGLLLLDQTKLPTEETYFVCRDVETVAQAIERL
                     AVRGAPAIGVSAAYGVVIGSMNADASNRREHILTAIERLRRTRPTAVNLFWALDRMKH
                     ILDDDAGDPTERLLAEARFIEQDDLRRSRAMGRHGASLLPQTATAITHCNAGGLATSG
                     YGTALGVFYAAQEAGKSVHVYADETRPLLQGARLTSWELAKAGIPVTVICDNMAAAVM
                     RREKIDAVFVGADRIAANGDAANKIGTYGLAIIAAKHSVPFYVVAPMSTVDLSLATGD
                     EIPIEERNEDEIRRGMGRQTVPDEAAVYNPAFDVTPFDLITGIITEHGIARAPFTDTL
                     HLWASRDEIHGADSTR"
     gene            3819..>4258
                     /locus_tag="ENN56_05135"
     CDS             3819..>4258
                     /locus_tag="ENN56_05135"
                     /inference="COORDINATES: protein motif:HMM:PF04390.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74910.1"
                     /translation="MNAIRGITGAIVSLAVAGCAYYSVTGGLPDHIKTVGVDTIVNET
                     VESGVDEMLYRALGDKLVGRPQFRYASSRLADAVIRGTIRNVVDEPFVYSGDRVSEYQ
                     IVIVGEAVLYDRVRRRTVWSNNTLRGIGKYDATGGTIAREEAIN"
BASE COUNT          935 a         1216 c         1255 g          852 t
ORIGIN      
        1 ccttgcggcg ttgggggata ttgtactcgt gatcgcgtac ggcctcgtac cggaacacgc
       61 cgcccgcgaa tggaaaccga cggtcgtcat cgtcgataag gacaatcgcg tagaagagat
      121 tcatactggt gcagagaacc gctctgcaac ttgacacggg tatcgcgcgt cgtattcttc
      181 acgaggaatg acgtacctta ttcgtaccaa cgaaaatggg atacggaggc acgtgtcaat
      241 gaagcacaca ttcgggattc tcgcggtcac ggcgttggtt attgccggaa tcgccgcggc
      301 atccgacaat tcgatcacag gtaatacacc aacatcgacg atggcgccga gctatcgtcc
      361 aactatagaa agcgcaccca cggtcgatct tatggggctg cttcagcgcc aagggatctt
      421 cgatcagaac cgcgtcagca cgtggcgtac gtactcgttt ggtatggcca tgggaggcgg
      481 tcagacgacg tccggcggct tactcgtgca gcatatgcag tatcaactga gcaaaccgct
      541 tacgcttcac gtcgcactcg ggctggagca caacccgctt ggtatggctg ggatgaatat
      601 cggcggtccg cagcaagcaa gtcttacgat cccatcggtg gacctgatct atcgtccgag
      661 cgattcgttt atcttctcgt tccactactc tcaagtcccg tcatatggtt acgactcggg
      721 gacgtggtgg gagcgtgaag cgatgtcgcc atattcatac ggttcgcggt ggtaacaacg
      781 tcgaccgaaa cgcattcacg gtttcgcatc gtctaccgat tggtagctgc gtttcttatc
      841 gcactcgttg tcgcggcgtg cacttcgtct gaacgagacg aaccgtcggt agacacggta
      901 tcacatgcac cgattgtcgt cgtattcaat ggatgcggtg caccgggtgc cgcgcgtgcg
      961 attgcagaag cgttacgggc cgaaggctgg gacatcggta acggttacgg tgagaacgcc
     1021 gagagctttg actatcccga tacattcgtc gtcgattacg ttggcgatcg tgagacggcg
     1081 caacgtatcg cagaaaagct cggcacgttc atgattcagc aaatcagcca cgatcccgat
     1141 cgattcggga ccgtaggcgt cattgtcgga gcggattacc gcgcgcgcgt cgcgcagtta
     1201 cttgagggtt gaatggttca gacggcacaa gcggaaaagc aggcagatcg aacgatttca
     1261 gacgatgtcg cgcgcctcgt ggaggctgca aggagcaaga aagctcgaga catcgccgta
     1321 ctggaccttc gaggatcgag tgacgttacc gacttcttcg tgatttgtac cgcggatgtc
     1381 gattcgcatg cccgcgcgat cgccgacggt attgtcgaat cgcagcgaat cgcgaccggc
     1441 cgtaaaccgt ggcatatcga aggcaccgat aagatgaact gggtgctcat cgattacgtc
     1501 gacgtcgtcg tccatatcat gcgggacgac acgcggaagt tttacggact ggaagagatt
     1561 tggggcgacg cgtcccgcac cgattacccg aacgaatagt atccgtagca tcccgtagta
     1621 ccgcggcgca tggtaggctc gtcgcgagcg gagttacgcg aacgatgtga gtatatcgat
     1681 cgcgaggcta tctggttcgc cggacagccg caggtgtcgt cgttgaccct gcggctgtct
     1741 ttatatccgg aggaattacc gcacggtgtc gcgcggtttt cacggcgtct actgcatatg
     1801 gaagaagagg aaacaggatg agcgattcgc tactcatcgt cggttcggtg gcgtatgatt
     1861 cagttgaaac acccacggga atacgcgtag atgcgctcgg cggttcggca acattcgccg
     1921 cggcggcagc atcgcttttc acaccggtcg accttgtcgg tatcgtcggt gatgacttcg
     1981 acctcgccgg tctcagcttc gtgactgatc gcggcgtcga tcttacgggt ctcgaaatac
     2041 gcaaaggcgg caagacgttt cgttggtccg gcgtgtacga aaccgacgtg aacattcgtc
     2101 acacgcgatc caccgacctg aacgtgttcg ccgacttcaa tccgacgctc cccgcgaaat
     2161 accgcagcga cgacgttgtg ttcctcggga acatgcttcc gtcgcttcaa cagcgcgtgc
     2221 tcgaacagac cacgaacccc atttgggtcg cgctcgatac catggatctc tggatcaaca
     2281 ccactcgcga ggaactcctc gacgtcatgc ggaaagtcca gatggttatc atcaacgact
     2341 ccgaagcgcg actcctcacg ggcgagtcga atctcgttcg cgcggcgcaa aacgtactca
     2401 cgctcggacc gaccgccgtc gtcgtgaaaa aaggcgagca cggcgtgttt ctcgtcatga
     2461 aggaaggatt cgccgtacta ccggcgctac ctctggaaaa cgtcgtcgat ccaaccggcg
     2521 ccggtgattc gtttgccgga gggatgctcg gcgcactcaa cgccacgcgc gatttctccc
     2581 tcgacggact caagcgcgcc atggcatacg gcacggcgac ggcgtcgctc acctgcgaag
     2641 cgttctccat cgacgcactg aagaccgcga accgcgatga actgaattac cgatatgaaa
     2701 aactcagagg catcgcgtca ttgccgccgc tgagtgtgtg acgatcggaa gactggattt
     2761 cgaatgccaa ctgaaacact gaaatggatc gacggcgaag gactcctcct cctcgatcag
     2821 acgaagctcc caacggaaga gacgtacttc gtctgccgcg atgtggagac cgtcgcgcaa
     2881 gccatcgaac ggcttgcggt gcgtggcgca ccggcaattg gcgtgtctgc tgcgtacggc
     2941 gtagtgatcg gttcgatgaa cgccgacgca tcaaaccggc gcgagcatat cctcaccgca
     3001 atcgagcgac ttcgccgcac gcgtccgacc gcggtaaacc ttttctgggc gctcgaccgg
     3061 atgaaacaca tcctcgacga cgacgccggc gatccgaccg agcgacttct cgccgaagcc
     3121 cgtttcatcg agcaggatga cctgcgccgc tcgcgcgcaa tgggccggca tggcgcgtca
     3181 ctcctcccgc aaaccgcaac cgcaatcacg cactgtaacg ccggtggctt ggcgacatcg
     3241 ggatacggaa ccgcgctggg tgttttctac gctgctcagg aggcggggaa atccgtgcat
     3301 gtctatgcgg acgagacacg tccgttactc cagggcgccc gactcacgtc gtgggaactc
     3361 gcaaaggcgg gaattcccgt gacggtgatt tgcgataaca tggctgccgc cgtaatgcgt
     3421 cgcgagaaga tcgacgcagt gtttgtcggc gcggaccgga ttgccgcaaa tggcgatgcg
     3481 gcgaacaaaa tcggcacgta cggtctcgcg atcatcgcag caaaacactc agtgccattc
     3541 tacgtcgtcg cgccaatgtc gacggtcgat ctgagcctcg ccaccggcga tgaaataccc
     3601 atcgaagaac ggaacgaaga cgaaatacgg cgcggcatgg gccgccagac ggtgccggac
     3661 gaagcggcag tctacaatcc cgcattcgat gtgacaccgt ttgacctgat caccggcatt
     3721 atcaccgagc acggcattgc gcgtgcacca ttcaccgata cgttgcattt gtgggcgtcg
     3781 cgcgacgaaa tccacggcgc cgactcgacg aggtaacaat gaacgccata cgcgggataa
     3841 ccggagcgat tgtctcgctt gccgtcgcag gttgcgcgta ttactcggtc accggtggct
     3901 tacccgacca tataaaaaca gtcggtgtcg acaccatcgt gaatgaaacc gtggaatccg
     3961 gtgtcgacga gatgctgtat cgcgcgctgg gggacaaact cgtcgggcgt ccgcagttca
     4021 gatacgcatc gtctcgcctc gcggacgcgg taattcgcgg aacgatccga aatgtggtcg
     4081 atgagccatt cgtgtattcg ggcgatcgcg tgagcgaata ccagatcgtt attgtcggag
     4141 aggcagtgtt atacgatcgc gttcgccgcc ggacggtttg gtcgaacaac accttgcgcg
     4201 gaatcggcaa atatgacgca acaggcggca caatcgcgcg agaagaagcg attaacag
//
LOCUS       DSCC01000414            2396 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1030128, whole genome shotgun sequence.
ACCESSION   DSCC01000414 DSCC01000000
VERSION     DSCC01000414.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1030128"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(51..1187)
                     /gene="bshA"
                     /locus_tag="ENN56_05140"
     CDS             complement(51..1187)
                     /gene="bshA"
                     /locus_tag="ENN56_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDS74911.1"
                     /translation="MKIGITCHHTYGGSGTVATELGMALADRGHQVHFITFERPYRLR
                     TDPNIYFHQVDVMHYPLFEHTPITLSLAAKMGEVLCTEALDLLHVHYAIPHATAAYLG
                     RQLCDRKNVRIVTTLHGTDITLVGQDPSYFSITRFSIEQSDAVTTVSQWLKDETIREF
                     QITRPIDVISNSVDTNRFHRMELDPTFRSKYAPNNEPIVVHISNFRPVKRVEDVIRIF
                     ARIREKMPAKLLMIGDGPMRMPAQQLATKLGVMNDTWFLGKQDEIEKYLSIADLFVFP
                     SQSESFGLAALEAMACGVPVVASRSGGLPEVIVNGQTGYLAEIGDIEHMAGHGIELLG
                     DRPLAQRISEASVQRVHEHFTHDTIVSRYVELYEREIELAASMV"
     gene            1341..2084
                     /locus_tag="ENN56_05145"
     CDS             1341..2084
                     /locus_tag="ENN56_05145"
                     /inference="COORDINATES: protein motif:HMM:PF03009.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74912.1"
                     /translation="MVNHVNVIAHRGASHARPENTLPAFEHAIELGADLLEFDVRLTA
                     DGHAIIMHDATVDRTTNGTGSVRSLTFDEIRELDAGMKHGTLYKDIRVPTLDEVLELA
                     RPSGVGLDVQIYATEDSRDELTKAVVEALNRYEYDDRAFLAAEEDVVLLARELDPKRP
                     ICNLTGQRDAKSLDRNKKTGSTIVQAFAKYVTPQFVEKAHKMGIVVNVFYADHVTEMR
                     RMLDNGVDGILTNEPEMLLRVLGRITDSP"
BASE COUNT          537 a          690 c          644 g          525 t
ORIGIN      
        1 cgataccggt tttcacagct ccgtagagca gccgcggatc gtcacgagtg tcacaccata
       61 gacgcggcaa gctcgatctc tcgttcgtac agctcgacgt atcgcgaaac gattgtatcg
      121 tgcgtaaagt gctcgtgtac tcgctgcact gacgcttcgg atattcgttg cgccagcgga
      181 cggtcaccga gcaattcgat cccatgtccc gccatgtgtt cgatatcgcc gatctccgca
      241 aggtatccgg tctggccgtt gacaatcacc tcgggcaatc cgcccgaacg cgacgcaacc
      301 acgggaacgc cgcacgccat tgcctcgagt gcggccaatc cgaagctttc cgactgactt
      361 gggaacacaa acaggtcggc gatactgaga tacttttcga tctcatcctg ctttccgaga
      421 aaccaggtat cgttcattac cccgagcttc gtcgcgagct gctgtgccgg cattcgcatg
      481 ggaccgtcac cgatcatgag cagcttcgca ggcatttttt cccgtatccg cgcgaatata
      541 cgaatcacgt cctcgacgcg ctttacgggt cggaagttcg atatatgaac gacaattggc
      601 tcgttattcg gtgcgtattt cgaacgaaac gtcgggtcca attccattct atgaaaccgg
      661 ttcgtatcaa cggaattgct aatcacgtca attggacgtg tgatctggaa ctcccgtatc
      721 gtctcgtctt tcagccattg acttaccgtc gtcaccgcgt ccgactgctc gatggaaaat
      781 cgtgtaatcg aaaaatacga cgggtcctgc cccacaagcg tgatgtcggt accgtgaagc
      841 gtcgtgacga tgcgtacgtt tttcctatcg cacagctggc gaccgagata tgcagcggtc
      901 gcatgaggaa tcgcgtagtg cacgtgaagc aagtcgagtg cttccgtaca gagcacttcg
      961 cccattttcg ccgcgaggga gagtgtaatc ggcgtgtgtt cgaagagtgg atagtgcatc
     1021 acatcgactt gatggaaata gatgttcgga tcggtgcgaa gtcggtacgg tcgctcaaat
     1081 gtgatgaaat ggacctgatg tccgcggtcg gcaagcgcca ttccgagttc cgtagcgacg
     1141 gtacccgatc cgccgtatgt atgatgacag gtaattccga ttttcaagat gaatcctccg
     1201 cacaggggag cggtaaaagc gttgaatgat tgttttcggt tctcggttca acgttcttta
     1261 gtgcacagcc ttcactaccc ggcgtgcatc tctccgcacc aaaatctacg atctgttctc
     1321 gtgacaggag acgaagttcc atggtgaatc atgtgaacgt tatcgcgcat cggggggctt
     1381 cccatgcccg tcccgagaac acgctaccag cattcgaaca cgcgatcgaa ctcggcgccg
     1441 acctcctcga gttcgacgtc cggctgacgg cagacggcca tgcgatcatc atgcacgatg
     1501 ccaccgtcga tagaacaacg aatggcacgg gttcagttcg cagccttacg tttgacgaga
     1561 ttcgcgagct cgacgcaggc atgaagcacg gtacgttgta caaggatatc cgggttccga
     1621 cactcgacga agttctcgaa cttgcacgtc cgagtggcgt tggcctcgat gtgcagattt
     1681 acgctaccga agacagccgc gacgagctca ccaaagccgt tgtcgaagcg ctcaatcgtt
     1741 acgaatacga cgaccgcgca tttcttgccg ctgaagagga cgtcgtgctt cttgcacgcg
     1801 agctcgaccc gaagcgaccg atttgcaatc tcaccggtca gcgcgatgcc aaatcacttg
     1861 accgtaacaa gaaaaccggc agtactatcg tgcaagcatt cgcgaaatat gtcacgccgc
     1921 aatttgttga gaaagcgcac aaaatgggaa tcgtggtgaa cgtgttctac gccgaccacg
     1981 taacggaaat gcgtcgtatg ctcgataacg gcgtcgacgg catccttacg aacgagccgg
     2041 agatgctgct ccgcgtactt ggccgcatca ccgactctcc gtagtccggt tcgccggccg
     2101 ctattcgtcg tcgaacgatt tacccgcgtg tgcgccttcg aactccttga gcagtccgag
     2161 ttcatcaagc agcttgcaga acggcaacat gtagcggaac cactgcatcg agtgcgcgtc
     2221 gcggcggaac cagcgcgcct cctccacaag cgccccacct gcatcatgga agaacacgcc
     2281 cggctggagg tccccaagcg atatcggtcc ggcgatcgac aggtaacgaa tccccgcatc
     2341 gaggaatcgt ttgtcgccgg tccattgata gacatacgcc gcgggttcca gcgcga
//
LOCUS       DSCC01000415            2462 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1029095, whole genome shotgun sequence.
ACCESSION   DSCC01000415 DSCC01000000
VERSION     DSCC01000415.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1029095"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..>2462
                     /gene="sprA"
                     /locus_tag="ENN56_05150"
     CDS             <1..>2462
                     /gene="sprA"
                     /locus_tag="ENN56_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HDS74913.1"
                     /translation="EDVGLDGCPDEYEDGLGGCLPTPTAVGDDPNGDNWVSPATLTDP
                     REFYGVNGTEGNRRDGNIYPTPDSEDLNGNGRLDVTNNYYSYTVDLRGFGRYAVEGSE
                     QKSGFRHFRIPVQALNVPEVGKIGDPPSFEQITGVRLWVTGVEDSELWLTIASLDFVG
                     SDWEEIKHQDDPFAVTTVNTQETPNYTPPPEAEVEYESDGTRRREQSLSLEFAALRPG
                     ESYAARRVFLKSKDFTNYRRMRMFIYGPFNTIDTGLDPMTDHLSAFLRFGTDSTSYYE
                     IEIPRLYPGWDARNYVDIPLDTLAQLKVDENGDVITESGVTRLYQSGRTLNRMRVVGY
                     QRGASLVLPTLSDVKMIEVGIANATTDRTIYAPNETKPIAVWFDDLHLLSARNLVGQA
                     ARGSMSIKMADFVSFQASATQQNVGFRTLQSVASGISGGRSSTAAQQASRNINLSMDR
                     LRIDKLLPPSWRVQLPVNMSYSTTSSTPRLKPNSDIPLVRREDKDTERRHSQVFNAST
                     SYTKTSRSPNPLLRLTLDRSTAGVTYSTSTGVTPTASARDSTKRTSYTARFGYDLTPQ
                     GTFKVKPLNWLGPLLPESMSSTEVTYLPSTLRFDASTTFSYDTTRSKKLNLPIDTTYA
                     SGRKTFSLSEAYRAQIQPLRSLSANYDLTFARDLQDEVVGRASPAEMAEVIAQSIFRE
                     NEIRRNHKFNISYSPPWPSWLTHAYSFSNTYTDDSDPRTSAGFTAAKEYSVNSQQSYN
                     LSNYTIRIRDILANFGGTGRSGSSGQRGGTNPASLPADSATSRGFTGGALRPTASFLS
                     RYIENITGRVGYTDQYTGAQLP"
BASE COUNT          580 a          685 c          703 g          494 t
ORIGIN      
        1 gaagacgtcg gtctcgatgg ctgtccggac gagtacgagg acgggttggg cggttgtcta
       61 ccgacaccga cggcggttgg agacgatccg aacggagaca attgggtttc tccggcgacc
      121 ttgaccgatc cgagggagtt ttacggtgtg aatggcacgg aaggtaatcg gcgagacgga
      181 aacatctacc caacaccgga ctcggaagac ctcaacggta acggacggct cgatgtaacg
      241 aacaattatt acagttacac agtcgatctg cgaggattcg gacgttatgc cgtcgaaggt
      301 tccgaacaaa agagcggatt ccgacacttc cgtatcccgg tgcaggcgct caatgtaccg
      361 gaggtcggga aaattggcga tccgccgtca tttgagcaga tcacgggcgt acgcctatgg
      421 gtaaccggag tcgaggactc cgaattgtgg ctgacgatcg cgagcctgga tttcgtgggc
      481 agtgattggg aggaaatcaa acaccaggac gatccgttcg ctgtcacaac agtgaacacg
      541 caagagacgc cgaattacac cccgcctccc gaagcggaag tggagtacga gtcggacgga
      601 acacgacgac gcgaacagtc cctctcgctg gaattcgcgg ctcttcgtcc gggcgaatcg
      661 tatgctgcgc ggcgcgtctt tttgaaaagc aaagatttca cgaactatcg gcggatgcgt
      721 atgttcattt acggcccgtt caacacgatc gacaccggtc tcgatccgat gacggaccat
      781 ctgagcgcgt ttctccgatt cggcaccgat tctacctcgt attacgaaat cgaaatcccc
      841 cgactgtatc cgggttggga tgcgagaaac tacgtcgata tcccacttga tacactcgca
      901 cagcttaagg ttgacgaaaa cggcgacgtt attaccgaat cgggagttac gcggctctac
      961 cagtcgggtc gaacacttaa ccggatgcgc gtcgtcggtt atcaacgcgg cgcgagtctc
     1021 gtgcttccga cgctcagcga cgtaaaaatg atcgaggtgg gtatcgcgaa cgcaacgacg
     1081 gatcggacga tttacgcacc gaatgaaaca aagccgatcg ccgtgtggtt cgacgacctt
     1141 cacttgctca gtgcgcggaa tcttgttggt caggctgcac gaggcagtat gagcatcaag
     1201 atggccgatt tcgtgagttt ccaagcatcg gctacgcagc agaatgttgg attccgaaca
     1261 ctccaaagtg ttgcgagcgg catatccggc ggacgctcat caacagcggc tcagcaggcg
     1321 agtcgaaaca tcaacctgtc catggatcgt ttgcgaatcg acaagctcct tcccccgagt
     1381 tggcgcgtcc aacttcccgt gaacatgagt tactcgacga caagcagcac gccgcggctg
     1441 aagccgaatt ccgacattcc gcttgttcgt cgtgaagaca aggataccga acgtcgtcat
     1501 tctcaggtgt tcaacgcgtc aacgtcgtac acgaagacga gtcgctctcc gaacccactt
     1561 ctcagactca ctctggatcg gtctacggcg ggtgtgacgt attcaacatc caccggcgtc
     1621 actccgacgg catcggcgcg tgatagcacc aaacgaacca gttacacggc gcgcttcggg
     1681 tatgatctga cgccacaggg gacattcaag gtgaagccgc tgaactggct cggtccactg
     1741 ctcccggagt ccatgagctc gacggaagtg acgtatctcc cgagcacgtt gcgattcgat
     1801 gcgtcaacga cgttcagcta cgatacgacg cggagcaaaa agctcaattt accgatcgat
     1861 acgacgtacg catcgggtcg gaagacattc tcgctttcgg aggcgtatcg cgcgcagatt
     1921 caaccgctgc gatcactgag tgcgaactac gatctcacgt tcgctcgcga cttgcaggac
     1981 gaggtggtcg gaagggcgtc accggctgag atggcggagg tgatcgcgca gagcattttc
     2041 cgtgagaacg aaatccgtcg gaatcacaag ttcaacattt cgtattcgcc accgtggcct
     2101 tcgtggttga ctcacgccta cagtttcagc aacacctaca cggacgacag cgacccgcgt
     2161 acaagcgcgg gctttacagc cgcaaaggag tactcggtca actcacagca atcgtacaat
     2221 ttgtcgaact atacgattcg cattcgagat atactggcga atttcggagg aactggacgg
     2281 tccggctcga gcggacaacg aggtggaaca aatcccgctt cgcttccggc cgactcggca
     2341 acgtcgcgcg gattcaccgg tggcgcgctt cggccgacgg catcattcct ttcgcggtat
     2401 atcgagaaca tcacggggcg ggtggggtat acggaccagt ataccggtgc gcaactcccc
     2461 ga
//
LOCUS       DSCC01000416            1598 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052261, whole genome shotgun sequence.
ACCESSION   DSCC01000416 DSCC01000000
VERSION     DSCC01000416.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052261"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            <1..993
                     /locus_tag="ENN56_05155"
     CDS             <1..993
                     /locus_tag="ENN56_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS74914.1"
                     /translation="NDVTYQYSVAGVDLSGNLGDPTNIASAKPAASEAPSAPTPQSPT
                     TRIIYADQISLTVKNSTRATSRPLVKSTYTFVIASDPELQTVIATVSKVTETSVNTST
                     LVAGNQFTANETYYWAARADDGIAVGPTSSVVSFTVAPVTITLQEFAAEGDANGAVHV
                     NWLLGGNNEGVSSVALFRGETSANGTPITTFGPEGTSAETIDYGVQLGTEPTYWLRVT
                     ETSGVTTEFGPVTAAMAVPEKWSLSPAYPNPFNPVTEMLLAVPEAGMATVTVYNILGQ
                     PVRTLWSGPITPGVHRLAWHGLDQQGRNAASGIYIVRAMSTTGVTMHQRITLIR"
     gene            1145..>1598
                     /locus_tag="ENN56_05160"
     CDS             1145..>1598
                     /locus_tag="ENN56_05160"
                     /inference="COORDINATES: protein motif:HMM:PF02113.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74915.1"
                     /translation="MRRFAAIITVLIGTLFAGCAPFTTREPMRIDPTHARAEAFYRGL
                     DSLVASPLLAPTHVGMAVWSLDRDEWIYEHQADKLFVPASTAKLIVAVSALHAYGPAR
                     RFETVVLTDSAVTDSTIPGNLYLVAGGAPDLTTGDLHALAERLRALGYT"
BASE COUNT          325 a          517 c          446 g          310 t
ORIGIN      
        1 aacgatgtga cgtaccagta ttccgtggcg ggtgtcgacc tctccggtaa cctcggtgat
       61 ccgacgaaca tcgcttcggc gaagccggcg gcatcggaag ctccgagtgc accgacgccg
      121 cagtcaccga caacgcgaat catctacgct gatcagatct cattgacggt gaaaaattcg
      181 acgcgcgcca cgtcgcggcc gttggtcaag tcaacgtaca cgttcgtcat cgcgtcagac
      241 cccgaactcc agacggtgat cgccacggtc agcaaggtca ccgagacgtc cgtcaacacc
      301 agcacgttgg tggcgggtaa ccagtttacg gcaaacgaga cgtattactg ggcagctcgc
      361 gccgatgacg gcatcgcagt cggtccgacg agttcggttg tatcgttcac cgtagctccc
      421 gtcacaatta ctctgcagga gttcgcggcg gaaggtgacg cgaacggtgc ggtccatgtg
      481 aactggctcc tcggcggaaa caatgaggga gtctcgtccg tggcgctgtt ccgcggcgaa
      541 accagtgcaa atggcacgcc gataacaaca ttcggtcctg agggcacgtc ggccgaaacg
      601 atcgattacg gtgtccagct tggcaccgag ccgacctact ggctccgcgt cacggaaacg
      661 agtggcgtaa cgactgaatt tggtccggtc accgccgcaa tggcggtacc ggagaagtgg
      721 tcactctcgc ctgcgtatcc gaacccgttc aacccggtaa ccgagatgct gctcgcggtt
      781 cccgaggcag gcatggcgac ggtcaccgtg tataacatcc tcggacagcc ggtgcgcacg
      841 ctctggtccg gtccgatcac gccgggcgtg catcgccttg cgtggcacgg tctcgatcag
      901 cagggacgta acgcggcaag cggtatctac atcgtccgcg cgatgagcac gaccggcgtc
      961 accatgcacc agcggataac actcatccgc taatcgaatc acctcaaagc gaaacgcccc
     1021 gacgtcgtca agatgtcggg gcgtttttct tgtaccacaa cacagaaaag tattgcaggc
     1081 ttttcgcaga actcacgata acggtatcct ttcactcgta cttcgatgtt ccaccgagga
     1141 gtgtatgcga cgcttcgccg cgatcattac cgttctgatc ggcactctct tcgccggatg
     1201 cgccccgttt accacacggg aaccaatgcg tatcgatccg actcacgcgc gtgccgaagc
     1261 attctaccgc ggcctcgaca gcctcgtcgc ctcacctctt ctcgcaccca cgcacgtcgg
     1321 catggcggta tggtccctcg atcgagacga atggatttac gagcatcagg ccgacaaact
     1381 cttcgtaccc gcttccacgg cgaaactcat cgtcgccgtc tcggcgcttc acgcgtacgg
     1441 tccggcgcgt cgtttcgaaa ccgtcgttct caccgactcg gcggtcaccg attcaaccat
     1501 tccgggcaat ctctatctcg ttgcgggcgg cgcgcccgac ctcacgacag gcgaccttca
     1561 tgccctcgcg gaacgtctgc gcgcgcttgg atacacac
//
LOCUS       DSCC01000417            4123 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1014551, whole genome shotgun sequence.
ACCESSION   DSCC01000417 DSCC01000000
VERSION     DSCC01000417.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1014551"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            18..671
                     /locus_tag="ENN56_05165"
     CDS             18..671
                     /locus_tag="ENN56_05165"
                     /inference="COORDINATES: protein motif:HMM:PF03147.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74916.1"
                     /translation="MDRKDGYSESRQISVLLTGKTPGDRWADTDRQWDWYDLRGVVEH
                     AIERCALLEPQFVSYDLNGYVQRTGVRIRIADDEVGEMGQVTPALCSEFDLTYPVFFA
                     RLDLAALTAHRRSLQKLTPLPRYPAVERDLALVVPESVPAGTVEDIVRKTGGALLERI
                     VLFDVYRGQGLREGEKSLGFTMEFRASDRTLTDQEVDELVRCVVSATEKSVGARVRT"
     gene            678..977
                     /locus_tag="ENN56_05170"
     CDS             678..977
                     /locus_tag="ENN56_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74917.1"
                     /translation="MDSQTRDPDAVFERFDSVIRETMMQLHRSRGERGQSELDRLNWE
                     LERDEMRRAAEERADVSKRAVTEPLRATLRSAVTRIDRILEGTSSNADGATSEDV"
     gene            974..1285
                     /locus_tag="ENN56_05175"
     CDS             974..1285
                     /locus_tag="ENN56_05175"
                     /inference="COORDINATES: protein motif:HMM:PF05164.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDS74918.1"
                     /translation="MSTSSQDRIVSTTVRIFGAEYSIRSPNESEYVQKVAELVDEAMR
                     RVSTHRVMRSNTEIAVLACMHIAGELLLAQEQLDEALNRIGDTTDALTRILDEETAEA
                     S"
     gene            1481..3046
                     /gene="rny"
                     /locus_tag="ENN56_05180"
     CDS             1481..3046
                     /gene="rny"
                     /locus_tag="ENN56_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDS74919.1"
                     /translation="MMELIGTVVAVLAIVAVAFALGWMLAKRLDRGRVGSAREMSKRM
                     IEDAKRESEALLHKVKLEARGIEIAAKEQLRIEGDKQRAELRRVEERLSDRESKLDRR
                     SDELQSREQSLNDRNRNLDNREKEINELQDSVDAIIDQQNRRLEEIANMTAEAAKEQL
                     IANMETKARSEGAHRAKEIRDRAIRDSQREAREIIGMAIQRYAGEHTTETTVSVVTLP
                     NEEMKGRIIGREGRNIRAFEMATGVDVIVDDTPEAVILSAFDPVRREVARLAMEKLVE
                     DGRIHPGRIEELVQKAQEQVEEEALKAGEEVVYELDIHDMNPQLIKTLGKLKYRTSYG
                     QNVLQHAKEVGILAGLMAEQLGLDTALARRAGVLHDIGKAVDRENEGTHVALGLDLVK
                     KYGEPAEVQNALASHHEDVVEGSMYCVLVKAADTISSARPGARRETIEGYVKRLDRLE
                     SIAQAFDGVEKTFAIQAGREVRVMVNCGKIDDAHAELLAGEIAEKIQADMEYPGQIKV
                     VVIRESRATSYAR"
     gene            3084..3908
                     /locus_tag="ENN56_05185"
     CDS             3084..3908
                     /locus_tag="ENN56_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDS74920.1"
                     /translation="MRVLFVGDILGKPGRRATEWWVPRLRRERNLDLVIANAENAAAG
                     TGITEKIVDELFDCGIDVITTGNHAWDKKDGHASIEEGRVLRPENYPPGVSGTGTIVA
                     HTHANEPVGVLNLQGRAFMRPIDCPFRVAKRQIDELRGETIAIIVDFHAEATAEKQAM
                     GWYLDGQVSAVLGTHTHVATADERVLPHGTAYITDVGMTGPHESVIGMEVDGAIRRLL
                     TGLNTHLAPAVADVRLAGVIVDIEASTGHAQAIERLMLPFDSRQSNEDSSDEEGSE"
     gene            3905..>4123
                     /locus_tag="ENN56_05190"
     CDS             3905..>4123
                     /locus_tag="ENN56_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDS74921.1"
                     /translation="MTAKVIDGRAISAQIRNEIKAVAEALEPTPHLHAVIVGDDPASA
                     LYVRNKERACASSGVRSTVHRLPATTSQD"
BASE COUNT          956 a         1049 c         1296 g          822 t
ORIGIN      
        1 agataggtac cgagtatgtg gatcggaagg acgggtattc tgaatcgcgg cagatctcgg
       61 tgttgcttac gggaaaaacg cccggagacc ggtgggcaga taccgaccgt cagtgggatt
      121 ggtatgacct gcgtggggtc gttgagcacg caatagaacg ttgcgccctt ctcgaaccgc
      181 aatttgtatc gtatgatttg aacgggtacg ttcagcgaac aggtgtgcgt atccgtatag
      241 cggatgacga ggtgggggaa atggggcagg tcactccggc tctgtgttcg gagtttgatc
      301 tgacttaccc ggtctttttc gcgcggcttg atcttgcagc gcttacggct catcgaaggt
      361 cgttgcagaa gctaaccccg ctcccgcggt atcccgctgt cgagcgcgac ttggcgctcg
      421 ttgtaccgga atcggtaccg gcgggcacgg tcgaagacat tgtgcgaaag acaggcggag
      481 cactgcttga acgcattgtg cttttcgacg tgtatcgcgg tcagggtttg cgtgagggcg
      541 aaaagagcct cggatttacc atggaattca gagccagcga ccgaacgttg actgatcagg
      601 aagtggatga acttgtgcga tgtgtagttt ccgcgacgga aaaaagcgtc ggcgcccgag
      661 ttcggacgtg agaaccgatg gattcgcaga cacgcgaccc tgatgccgta tttgagcggt
      721 ttgactccgt aattcgcgag acgatgatgc agcttcatcg ttcacgtggc gaacgtggtc
      781 agagcgaact cgatcggctc aactgggaac tcgagcgcga cgaaatgcgt cgtgccgccg
      841 aggagcgtgc ggacgtttca aaaagagctg ttactgagcc gctccgagcg acgttacgtt
      901 cggcagttac gcgaatcgat cgtatcctcg aaggaacgtc gtcaaatgct gacggagcga
      961 cctcggagga tgtatgagca cgtcatcgca ggaccgtatt gtttcgacga ccgtgcggat
     1021 tttcggcgcc gagtattcga ttcgttcacc gaacgagtcg gaatacgttc agaaggttgc
     1081 cgaactggtt gatgaagcga tgcgtcgcgt gagcactcat cgagtaatgc gctcgaatac
     1141 cgaaatcgcg gttctcgcgt gcatgcacat agccggagag ctcttgcttg cgcaagaaca
     1201 actcgatgaa gcgctcaatc gaattggcga cacgaccgac gcactcacac gtatcctcga
     1261 cgaggaaact gcggaagcat cctgattagc accgacggtt aacggcgtat tccacctcct
     1321 ccgctacggg acggatgtcg acacgcgaac gaatccacac agtggatcga tcgtgttgcc
     1381 gatgttgtca tgtccctcgg cgcaggaggt tttttgttgc ttcgccgcgc gttcattggg
     1441 cagttgcggc gtatgataga agtgagttcg gaggaaggtt atgatggagt tgatagggac
     1501 ggtagttgcc gttctcgcga tcgtcgctgt cgcgtttgca ctcggatgga tgcttgcaaa
     1561 gcgacttgac cgtggtcgcg tcggtagtgc gcgtgagatg tcgaaacgga tgatcgaaga
     1621 cgcgaaacgc gaatcggagg cactgcttca caaagtgaag ctcgaagcgc gcgggatcga
     1681 gattgcggca aaggaacagc ttcgtatcga aggtgacaag cagcgcgccg agcttcggcg
     1741 cgtcgaggaa cgactatcgg accgcgaatc gaagctcgat cggcgctctg acgagttaca
     1801 gtcgcgcgag cagagcctca acgaccgcaa tcggaatctt gataaccgcg agaaagagat
     1861 caacgagctt caggatagcg tcgatgccat catcgatcag cagaatcgtc gccttgaaga
     1921 gattgcgaac atgaccgccg aggcggcgaa agagcagctt atcgcaaaca tggagacgaa
     1981 ggcgcgttcc gaaggtgctc accgcgcgaa agaaattcgc gaccgcgcga tccgagattc
     2041 gcaacgtgag gcgcgcgaaa tcattggaat ggcgatccaa cggtatgcag gtgaacacac
     2101 cacggaaacg acggtatcgg tggtgacact gccgaacgag gaaatgaaag gtcggatcat
     2161 cggtcgcgaa gggcggaata tccgcgcatt cgaaatggca accggtgtgg atgtcatcgt
     2221 cgacgatacg ccggaagcgg tcattctctc ggcgttcgac cctgtacgac gcgaagtcgc
     2281 gcgactcgcg atggaaaaac tcgttgaaga cgggcgtatt catcccggac gcatcgaaga
     2341 gctcgttcag aaagcgcagg agcaagttga agaggaagcg ctcaaagccg gagaagaggt
     2401 tgtctacgag ctcgacatcc atgacatgaa cccgcagctt atcaagacgc tcggtaagct
     2461 caaatatcgt acgagttatg gtcagaatgt tttacagcat gcgaaggaag ttggtatcct
     2521 cgccggtctc atggcggagc aactcggttt ggatacagcg cttgcgcgtc gagcgggcgt
     2581 gcttcacgat attggcaagg ctgtcgatcg agaaaacgaa ggcacccatg tcgcacttgg
     2641 cctcgatctc gtgaaaaagt acggcgaacc ggcggaagtc cagaacgcgc ttgcgtcaca
     2701 tcatgaggat gtcgttgaag ggtcgatgta ctgcgtgctt gtcaaggccg ccgacacgat
     2761 atcgagtgcg cgtccgggtg ctcgtcgcga gaccattgag gggtacgtaa aacgccttga
     2821 ccgtctcgag tcgattgcgc aggcattcga cggtgttgag aagacgtttg cgattcaagc
     2881 gggtcgagaa gtgcgtgtca tggtcaactg tggaaagatc gacgatgcgc acgcggagct
     2941 gcttgcaggt gagattgcag agaagattca agcggatatg gaataccccg gtcagatcaa
     3001 ggtggtcgtg attcgggagt cccgtgctac gtcgtacgcg cggtagaagt ttcgtcaggt
     3061 ctctttcgcg gattggatgg cgcatgcgtg tactgttcgt cggagacatt cttgggaaac
     3121 ccggacggcg tgcaaccgaa tggtgggtgc cgcgtttgcg acgagagcgg aatctcgacc
     3181 ttgtcattgc gaacgcagag aatgcagccg ccggcactgg tatcaccgag aagatcgtcg
     3241 atgagctctt cgattgcggt atcgacgtta tcaccaccgg aaaccacgcg tgggacaaaa
     3301 aagatggaca cgcgtcgatt gaagagggac gagtactccg acccgaaaac tatccacccg
     3361 gtgtaagcgg caccggtacg atcgtcgccc atactcacgc gaacgaaccg gtcggagttc
     3421 tcaatctgca aggccgcgcg ttcatgcgtc caatcgactg tccgtttcgt gtcgcaaaac
     3481 ggcaaatcga tgaattgcgt ggcgagacta ttgcgatcat tgtagatttc cacgcagaag
     3541 cgacggcgga aaaacaggcg atggggtggt acctcgatgg acaggtgtcc gccgtactgg
     3601 gaactcacac ccacgtcgct accgcagacg agcgcgtact cccgcacggt accgcgtata
     3661 ttaccgacgt ggggatgacg ggcccgcacg aatcggtgat cggtatggag gtcgacggcg
     3721 cgattcggcg cctcctgacc ggattgaaca cgcatctcgc gccggcggta gccgatgtgc
     3781 gactcgccgg cgtgatcgtc gatatcgaag cgagcaccgg tcacgcacag gcgatagagc
     3841 ggcttatgtt gccgttcgat tcacgtcaga gtaatgagga ctcgagtgac gaggagggga
     3901 gcgaatgacc gcaaaagtca tcgacggccg agcaatctcg gcgcagattc gtaacgaaat
     3961 caaagcagta gcggaagcac tcgagccgac accgcatctc cacgcggtga tcgtcggcga
     4021 cgaccctgct tcggcgctct atgtgcgtaa taaggaacgt gcctgcgcat cctccggtgt
     4081 tcgcagcacc gtgcaccgcc tacccgcaac gacatcgcag gac
//
LOCUS       DSCC01000418            1953 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039772, whole genome shotgun sequence.
ACCESSION   DSCC01000418 DSCC01000000
VERSION     DSCC01000418.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039772"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..>1953)
                     /locus_tag="ENN56_05195"
     CDS             complement(<1..>1953)
                     /locus_tag="ENN56_05195"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02518.24,HMM:PF02895.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDS74922.1"
                     /translation="SELHRVFLGEARETLETLGRNIIDLERDAHNTDTVNSALRLAHT
                     LKGSAAMVGFDRVRVLSHAMEDSLQAVRDGRVDLTADMTDMLLGALDVLARYVAAIGE
                     TGTEPSDSRSNEAVQRLRGFAETPSPPTSTIQQDPTPSDSPADVGGSLAEPDVVESED
                     EISEECIEEEITPEDFPRWSHDVFVELIRSLVALDGDASNTVIRGHFAVTAKRLAHGA
                     ELSGYDKIDDLASALAQLMCDGDSREPVSDDLLDVLNAGVDALGMALVRVRRDGHDRD
                     PAVDEVTSLLSTVDLGDPRAEASDASDNGTAKNDERRGRSRTVEVDLDRLNRLLSLAA
                     ELVIVPNRLSAELERVVERIVRMTNGYATRSDRPEPSEQSQAVGLDAVPQTSDVNSTR
                     RNGDWELAEITESIGEFGDATANLDQDIGRINTIAKDLHDEILRVRMVRVERAFDRIP
                     LIIRDAARSENKRVNLVLEGADTEIDKNILEAMNNPLLHLIRNTVGHGIETPEERIAV
                     GKPPIGEVRVKAEQDGNRIVVEVSDDGRGIDPDRIRSTAVRRELLTSEEATSLTDAEA
                     INLIFRPGFTTVEAVNELSGRGVGLDVVRSVATRFNGSVIVESTVGRGTVFRVTLPLT
                     LAIGQALLVEVCDTIFALPLPAVDRI"
BASE COUNT          397 a          596 c          535 g          425 t
ORIGIN      
        1 caattcggtc gacagcggga aggggaagtg cgaatatcgt atcgcagacc tctacaagaa
       61 gcgcttggcc gattgcgagt gtaagcggta gcgttacgcg aaatactgta ccgcgtccga
      121 cggtgctttc cacaataacg gaaccattga accgcgttgc cacgctacgc accacgtcga
      181 ggccgacacc acgaccagag agttcgttta ccgcctcaac agtggtaaaa cccggacgaa
      241 agatcaaatt gatcgcctcg gcgtccgtca gcgacgttgc ctcctcggac gtgagcaatt
      301 cgcgtcgtac tgcggtactt cggatgcgat ccgggtctat tccgcgaccg tcgtcactga
      361 cttcgacgac tatccggttc ccatcctgct ccgctttgac gcgaacctca ccgatcggcg
      421 gcttaccgac agcgatccgt tcctcaggcg tttcgatacc gtgtccgacc gtattccgaa
      481 tcagatgaag caacgggtta ttcatcgctt cgagaatgtt cttgtcaatc tcggtatcgg
      541 caccttccaa cacgagatta actcgtttgt tttcgcttcg tgccgcgtct cgaataatca
      601 aaggaatccg gtcgaaagct cgctcgactc gcaccattcg gacgcggagg atctcatcgt
      661 gaagatcctt cgcaatcgtg ttgatacggc cgatgtcttg atcgaggttc gctgttgcgt
      721 cgccgaactc gccgatcgat tccgtgatct ctgccaattc ccaatcgccg ttgcggcgtg
      781 tcgaattgac gtcgcttgtt tgaggcaccg cgtcgagtcc aaccgcctgc gattgttccg
      841 aaggttccgg gcgatcactg cgtgtcgcgt acccgttcgt catgcgaacg atgcgttcga
      901 ctacacgttc aagttcggca ctcagacggt tcggaacgat caccaactcc gcggcgaggc
      961 tcaacagccg attcaggcgg tcgaggtcaa cttcgactgt gcgagatcgt ccccgtcgct
     1021 cgtcattttt cgcggtgccg ttatctgacg cgtcggaagc ctccgcacgc ggatctccga
     1081 ggtcgacagt actgagtaac gaagtgacct cgtcgaccgc tggatcgcgg tcatgcccat
     1141 cgcgtcggac gcgaacgagt gccattccca acgcatcgac gcccgcgttg agcacatcga
     1201 ggagatcatc cgagaccggt tcccgggaat cgccgtcgca catcaactgc gcaagcgccg
     1261 acgcgagatc gtcgatcttg tcgtaccccg aaagctccgc tccgtgcgca agacgctttg
     1321 ccgttacagc gaaatgtccg cggattacgg tattcgacgc atctccgtcg agagcaacga
     1381 gcgaccgtat cagttccacg aacacatcgt gtgaccaacg tgggaaatct tccggcgtga
     1441 tctcctcctc gatacactct tcggagatct catcctcgga ctctacgaca tcgggctctg
     1501 caagagatcc ccctacgtcg gcaggcgaat ccgaaggtgt cggatcctgt tgtatcgtag
     1561 acgtcggtgg agaaggtgtt tcggcaaacc cgcggagccg ctgaacagct tcgttcgagc
     1621 gtgaatcgct tggctctgta ccagtttcgc cgatcgccgc cacataccga gcgagcacat
     1681 ccagtgcacc gagaagcatg tcggtcatat ctgcggtcaa gtcgacgcga ccgtcccgca
     1741 ccgcttgaag tgagtcttcc atcgcgtgtg agagcactcg gacccggtcg aaaccaacca
     1801 tcgccgccga gccctttagt gtgtgagcaa gtcggagtgc gctattgacg gtatcggtat
     1861 tgtgtgcgtc gcgctcgaga tcgatgatgt tgcgacccag cgtttcgagc gtctcacgcg
     1921 cctctccgag aaacacgcgg tgcagctcag aca
//
LOCUS       DSCC01000419            1581 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052985, whole genome shotgun sequence.
ACCESSION   DSCC01000419 DSCC01000000
VERSION     DSCC01000419.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052985"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            15..539
                     /gene="hslV"
                     /locus_tag="ENN56_05200"
     CDS             15..539
                     /gene="hslV"
                     /locus_tag="ENN56_05200"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDS74923.1"
                     /translation="MRSTTILGVQRNGEVAMGGDGQVTLGNTVVKHETVKIRKLNNDS
                     VLAGFAGGAADAFTLLERFEGKLKEYRGSLPRAALELAREWRTDKFLRHLEAFLIVMD
                     CENMFILSGNGDVIQPESGVAAIGSGGSYALAAAHAYLDASDLSVREIVERSMRIAGR
                     FCIYTNDHITVETL"
     gene            582..1493
                     /locus_tag="ENN56_05205"
     CDS             582..1493
                     /locus_tag="ENN56_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDS74924.1"
                     /translation="MSQDPLLLVCNKHDDTMSFVDPVALRVIETIPVGPNPHEIIVTP
                     DRCFAYLSNYRPPGDSISVIDLDARRHTKQIATGDYTRIHGAAMTSDGSFAYFTAGQS
                     GYVIEIDTSENIVTRAISTCGEISHMVYVSSDDTLLYTANIVSEDVSVIDRASGELLR
                     KIPCGSGVEGMAFSPDGTELWALNQTGDSIDVIALPSHEVVERITCPGMPVRCVFSPD
                     GSRAYIPSWTESGELIVIDSATRKEIARIPVGSQAIGVVISPDGGRVFVGCEHTDGVH
                     VVDTATLDVIDVVHTGDGSDAMAYVSA"
BASE COUNT          337 a          457 c          459 g          328 t
ORIGIN      
        1 cgccgaatcg accgattcgc tcgacgacca ttctcggagt acagcgcaac ggcgaagtcg
       61 ccatgggcgg cgatggacag gtgacgcttg gcaacaccgt cgtcaagcac gagacggtga
      121 agatccgcaa gctcaataac gacagtgtgc tcgccggatt cgccggtggt gcggccgacg
      181 cgttcaccct tctcgagagg ttcgaaggaa agctgaagga ataccgtggc agccttccgc
      241 gcgcggcact cgagctggcc cgtgaatggc gcacggataa attcctccga catctcgaag
      301 cgtttctcat cgtcatggat tgcgagaaca tgttcatcct ctccggtaac ggcgacgtga
      361 ttcagcccga atcaggtgtc gcggcgatcg gatccggtgg ttcgtacgcg cttgcagccg
      421 cgcacgcgta tctcgacgca tccgatctga gtgtgcgcga gatcgtagag cgaagcatgc
      481 gtatcgccgg tcgtttttgc atctacacga acgaccacat cactgtcgaa accctataac
      541 gcggcccgag gtcgtcggcc gataccttcg gaaagctccg tatgtcccaa gacccgcttc
      601 tcctcgtctg caataaacac gatgacacca tgtcattcgt cgaccccgtc gcactccgcg
      661 taatcgagac gatacctgtc ggaccgaacc cgcacgaaat catcgtaacg ccagaccgat
      721 gtttcgcgta cctctccaac taccgtccgc ccggcgattc gattagcgtt atcgaccttg
      781 atgcgcgtcg gcacaccaag caaatcgcga caggagatta cacgcgaatc cacggtgcgg
      841 cgatgacgtc cgacggaagt ttcgcgtact tcaccgccgg tcaatccggt tacgtcatcg
      901 agatcgacac gtcggaaaat atcgtgacac gggccatttc gacatgtggc gaaatatcgc
      961 acatggtgta cgtttcttcc gacgacacgc tcctctacac cgccaacatc gtctccgagg
     1021 acgtttccgt gattgatcgc gcctccggtg agttgcttcg gaagatcccg tgcggttcgg
     1081 gagtcgaagg tatggcgttt tcgcccgatg gaaccgagct gtgggcgctc aatcagaccg
     1141 gtgacagtat cgacgttatc gcacttccgt cgcacgaggt cgttgaacgg atcacgtgtc
     1201 cggggatgcc ggttcgctgt gtgttttctc cggacggaag ccgcgcgtat atcccgagtt
     1261 ggacggaaag cggcgagctg atcgtgatcg attcggcaac acggaaggaa atcgcgcgga
     1321 ttccggttgg ctcacaggcg atcggcgtgg tgatatcgcc ggacggaggc cgtgtttttg
     1381 ttggatgcga gcatacggat ggtgtacacg tagtcgatac ggcgacgctt gacgtgatcg
     1441 acgtggttca caccggtgac ggctccgacg cgatggcgta cgtttccgcc taacttcagc
     1501 caatacaaaa agaaacgcgc cgaacgaatg agacatcgta cggcgcgttt ttatgtacgg
     1561 tatgagtcag tgcgtcagaa c
//
LOCUS       DSCC01000420            1952 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1039796, whole genome shotgun sequence.
ACCESSION   DSCC01000420 DSCC01000000
VERSION     DSCC01000420.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1039796"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..996)
                     /locus_tag="ENN56_05210"
     CDS             complement(<1..996)
                     /locus_tag="ENN56_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDS74925.1"
                     /translation="MFKKILIANRGEVAVRIMRACKELGIATVAVHSEGDRQSLHVRA
                     ADEDVCIGPSDASRSYLDPVRIISAAEVTGADAIHPGYGFLAENARFVDICDECDITF
                     IGPSGDMIRRMGDKAVAKKTMADAGVPVVPGSDGPLKSLDEARVLGSEIGYPVILKAV
                     SGGGGKGIRVIENEDQLARSYDIARNEARSAFSDDSLYLEKFIDRARHIEVQILGDAH
                     GNTVHLGERDCTVQRRRQKLIEESPSPVVSPELREAICTTAVRGAANIGYTNAGTIEF
                     LLTESGEFYFMEMNTRVQVEHPVTEMVTGIDIVREQIRIASGLPLGFTQDDIRMQG"
     gene            complement(1026..1490)
                     /gene="accB"
                     /locus_tag="ENN56_05215"
     CDS             complement(1026..1490)
                     /gene="accB"
                     /locus_tag="ENN56_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDS74926.1"
                     /translation="MTLDEIRQLIELVQKSEIAELRINRGDDTIRITRQFPVQVVEAT
                     PNVAPGTPGTPPATATASAPADEPVSAAQSGLYDVTAPMVGTFYRAPSPESPVYINVG
                     DHIDAGQIICIIEAMKLMNEIPADRSGTVVEIAIENAQPVEFGQTLIRIKPD"
     gene            complement(1601..>1952)
                     /locus_tag="ENN56_05220"
     CDS             complement(1601..>1952)
                     /locus_tag="ENN56_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDS74927.1"
                     /translation="QYLYHDGDFYVFMDTGTYEQIHLDSTKVDDVADLMKENEIVTVV
                     FGDERPLAVELPFHVDLKIVETDPGVKGDTATGGTKPAKLESGASVQVPLFIQVGERI
                     RVDTRTREYLSRVN"
BASE COUNT          391 a          559 c          544 g          458 t
ORIGIN      
        1 accctgcatg cgaatgtcgt cttgtgtaaa gccaagtgga agccccgatg caattcgaat
       61 ctgctcacgc acaatatcga ttccggtaac catctccgtt acgggatgct ccacctgtac
      121 gcgagtgttc atctccatga agtaaaactc gccagattcc gtgaggagaa actcgatggt
      181 tcccgcgttc gtatagccaa tattcgccgc acctcgcacc gccgtagtgc agatggcttc
      241 gcgaagctca ggtgatacaa ccggcgatgg cgattcctcg atcaacttct gacgacgacg
      301 ctgaacggtg cagtctcgct ctccgagatg cacggtgttt ccgtgtgcgt cgccaagtat
      361 ctgcacctcg atgtgtcgcg cgcggtcgat gaacttttcc agatagagcg agtcatcact
      421 gaatgctgac cgggcttcgt tccgggcgat gtcgtatgat cgggcaagct gatcttcgtt
      481 ctcgatgaca cgaatgccct tgccgccgcc gccggatact gctttcagga tgaccggata
      541 cccgatttca ctgccgagta cccgagcttc atcgagggac ttgagtggtc cgtctgagcc
      601 gggaacaacc ggtacaccag catccgccat ggtcttcttc gcgacggctt tgtcacccat
      661 gcgcctgatc atgtcgcccg acggaccaat gaacgtgatg tcgcactcgt cgcagatatc
      721 gacgaaccgt gcgttttccg cgaggaaccc gtatccggga tggatggcgt ccgctccggt
      781 aacttccgca gcagagatta tgcgaacggg atcgaggtaa ctacgggatg cgtctgaggg
      841 accgatacac acatcttcgt cggccgcacg gacgtggagc gactggcggt cgccttcgga
      901 gtgcacggcg acagttgcta tcccgagttc tttgcacgcg cgcatgatgc gaaccgctac
      961 ttcaccgcgg ttcgctataa ggattttctt gaacatgtac gtcctgatga accggcgagg
     1021 atcggtcagt ccggtttgat gcgaataagc gtttgtccga actctacagg ttgtgcgttc
     1081 tcaatcgcga tttcgaccac cgtacccgaa cgatctgcgg ggatttcgtt catgagtttc
     1141 atcgcttcga taatgcagat gatctgtccg gcatcgatgt ggtcaccgac gttgatatac
     1201 accggtgatt cgggcgaggg cgctctatag aacgttccca ccattggcgc tgtcacatca
     1261 taaagcccgg actgtgcggc actcacgggt tcgtcggccg gagccgacgc agtggctgtg
     1321 gccggtggag tacccggggt ccctggtgcg acgttcggtg ttgcctctac gacctgcacg
     1381 ggaaactgcc gtgttattcg gatcgtgtcg tcgccgcgat tgatgcggag ctccgcaatc
     1441 tcgcttttct gtacaagctc gatcagctgt cgaatttcat cgagtgtcac gagaggctaa
     1501 ccctccttcg tcgcgtcatc gccaacgtgt acgcgacggc gtatatcgta ttctcgccga
     1561 gtatgatagg agcattgtcg gcgagtcgcg atgtcctgaa tcaattgacg cgggatagat
     1621 actctcgcgt gcgagtgtcg acgcgtatgc gttcgccaac ctggatgaat aacggaacct
     1681 gaaccgaagc gccggattcc agcttcgccg gcttcgttcc accggtggcg gtgtcgcctt
     1741 tcacaccagg atcggtctcg acaattttca gatcgacatg gaacggcagc tcaaccgcga
     1801 gcggacgttc gtcaccgaac acaacggtca cgatttcatt ctctttcatc aagtccgcaa
     1861 catcgtctac cttcgtcgag tcgagatgaa tctgctcgta cgttcccgtg tccatgaaca
     1921 cgtagaagtc gccgtcgtgg tagagatact gc
//
LOCUS       DSCC01000421            2880 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1023623, whole genome shotgun sequence.
ACCESSION   DSCC01000421 DSCC01000000
VERSION     DSCC01000421.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1023623"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            complement(<1..2782)
                     /locus_tag="ENN56_05225"
     CDS             complement(<1..2782)
                     /locus_tag="ENN56_05225"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74928.1"
                     /translation="MYNWEFVQTPHFDIYFHDGMEGVARIATTVAEEAYDQISASFDY
                     RLRNRVPIIIYGSRTDFRETHLTSGLVPEGTGGFTEFLKNRVVVPFEGSYPSFRHVLH
                     HELTHAVLLDMLYGKDILQGIQNSDIPLWFIEGLAEYESIPWTAESDMWMRDAVINDY
                     LNLGGYFAYKGGQSIMYYIAEVYGPEKIPQMLHRLRSFGRFGTTMQAVLGVELEEFGK
                     QWEKDLKKVYWPEIVDREDPDEVAEALTDHTKDDSYFNRNPAFSPYGDKIAFFSGRDY
                     YTSIYVMSAIDGKILWKVVTGEQTGTYEEMHWQRGQITWSPDAKQLAFAAQATDGDRL
                     YIVDARTGKQERTFQPPFDGVYTPAWSPDGSKIAFIGLNKGLADLYILELSDGSITQL
                     TNDRFNESNPAWSPDGAMIAFDSDRAPGIRDTSPDLLYQTSDVYVIEVSTGRMRRLTS
                     HERNDTSPTWSPDGRHIAFVSDRNGIFNLYLGEFDSGTGTLERTIRPLTNILTGTFFP
                     SWSPTGDKIAFSGFNQGGWDIYVVRNPLEKLKDDSLEPAFFLKQERISYRSEYRHLSA
                     EGHTVDADSTVAYRIGPRMATLRPESWNIVDDTMTDPARGGFRTRKYSPKLEIDMVTG
                     YAAYSTLGGVGGELLITASDLMSHHNLAFTTSLYRDLRDSDYALLYQYLRRRTNYAVY
                     ISQQRTWYPFRDYVVVGNQIGIESGLVERRELYGGVYASRPTSQYRRLEFGVEGMRAE
                     TSVLYDQIATLNPGSPSQEYSTDAFLANAAYVHDNTLWSSFWAVKGSRWRFQLRSAPP
                     YANTAPFGVATADWRKYIRVGRNSSFAFRLSGGASFAEDGNAPVFYLGGLPYWINYRY
                     GDVPASTFSNDSFSTFVFPLRGTDYYEMSGRRYALMNLEYRFPLIYFLAARFTFQMMP
                     SNVGGVLLLDLAT"
BASE COUNT          644 a          773 c          791 g          672 t
ORIGIN      
        1 cagtcgcaag gtcgagaaga agtacgccgc cgacattgct gggcatcatt tggaacgtga
       61 aacgcgccgc aaggaaatag atgagcggga atcggtattc aaggttcatc aaggcgtagc
      121 gccgaccact catttcgtag taatcggtac cgcgcaatgg aaatacgaac gtcgaaaatg
      181 aatcgtttga gaacgtcgat gccggcacgt cgccgtagcg gtagtttatc caatagggaa
      241 gaccacccag atagaaaacc ggtgcgttac catcctccgc gaaacttgca cctccgctca
      301 gccgaaacgc gaaactcgaa ttccgaccga ctcggatgta tttgcgccaa tccgccgtag
      361 cgacgccgaa cggtgcagtg ttcgcatacg gcggagcgct gcgaagctga aaccgccacc
      421 gcgatccctt caccgcccaa aagctcgacc agagcgtgtt atcgtgtaca tacgcggcgt
      481 tcgccaaaaa cgcgtcggta ctgtattcct gcgacggcga gccgggattc aatgttgcga
      541 tttgatcgta gagcacggag gtttcggcac gcatcccttc tacgccgaac tccagccggc
      601 gatactggct tgtaggacgc gatgcataca caccgccgta gagttccctt cgctcgacga
      661 ggccggattc gataccgatc tggtttccca caacgacgta atcgcggaac ggataccacg
      721 ttcgttgctg actgatatat accgcgtagt tcgtgcggcg ccgtaggtat tggtagagta
      781 atgcgtaatc gctatcgcga agatcgcgat agagtgacgt tgtaaacgcc agattgtgat
      841 gcgacatgag atccgacgcg gtaatgagca gttcgccacc gacaccgcca agtgtcgaat
      901 aagcggcgta tcccgttacc atatcaatct caagtttcgg cgagtacttt cgcgttcgga
      961 acccaccgcg ggccggatcg gtcatcgtgt catcgacgat gttccacgat tcgggtcgaa
     1021 gcgtcgccat ccgaggcccg atgcgatagg caaccgtgga gtccgcatcc acagtatgcc
     1081 cttcggcgct gagatgccgg tattcgcttc ggtaagaaat gcgttcttgc ttgagaaaaa
     1141 atgctggttc gagcgaatcg tctttgagct tttcaagggg attccgaacg acgtagatat
     1201 cccatccacc ctgattgaat ccggaaaatg cgatcttgtc ccccgtcgga ctccacgaag
     1261 ggaagaacgt gccggtgaga atattcgtca gaggtcgaat cgttcgttca agtgttcccg
     1321 tgccggaatc gaattcgccg aggtacagat tgaagatccc gttgcggtcc gaaacgaacg
     1381 caatatgacg accgtccggg ctccacgttg gagacgtgtc gtttcgttcg tgtgaggtaa
     1441 ggcggcgcat ccgacccgta gacacttcaa ttacatacac atccgaggtc tgatacaaca
     1501 aatcaggcga tgtatctcgt atgcccggcg ctctatcgga gtcgaaggcg atcattgcgc
     1561 cgtccggtga ccatgcgggg ttgctttcat tgaaacggtc attcgtcaac tgtgtgattg
     1621 aaccgtctga tagttcgagt atgtagagat cggccagtcc cttgttcaga ccgataaatg
     1681 cgatcttgga accgtctggt gaccacgccg gagtatatac accgtcgaac ggtggctgga
     1741 atgtgcgctc ttgtttcccg gtgcgcgcgt cgacaatata gagacgatct ccgtctgtcg
     1801 cctgagccgc gaacgcaagc tgctttgcgt ccggcgacca cgtaatctgg ccgcgttgcc
     1861 aatgcatctc ttcgtaggtg ccggtttgct ctcccgtgac gaccttccag aggatttttc
     1921 cgtcgattgc cgacattacg tagatacttg tgtagtagtc gcgtccactg aaaaaggcaa
     1981 ttttgtcgcc gtacggactg aacgcgggat tgcggttgaa atacgaatcg tctttcgtgt
     2041 gatcagtaag cgcttcggcg acttcgtccg ggtcttctcg atcgacgatt tcgggccaat
     2101 agaccttctt cagatccttt tcccactgtt tgccgaactc ctccaactct accccgagaa
     2161 ccgcttgcat cgtggtgccg aaacgtccga agcttcgcag gcgatgcagc atctgcggga
     2221 tcttttccgg cccgtacact tcggcgatgt agtacatgat cgattggccg cccttgtacg
     2281 cgaagtatcc gcccagattg agatagtcgt tgatcactgc gtcgcgcatc cacatgtcgc
     2341 tttccgcggt ccacggaata ctttcgtact cggcgagtcc ttcaataaac cataggggga
     2401 tgtcgctgtt ttgaatgccc tgtaggatgt ctttcccgta gagcatgtcg agtaaaacgg
     2461 cgtgtgtcag ttcgtggtgc aggacgtgcc ggaacgatgg gtatgagcct tcaaaaggaa
     2521 cgacgacacg gttttttagg aactcggtaa aaccgccggt gccttccgga acaaggcccg
     2581 atgtgaggtg cgtctcacga aaatcggttc gcgagccgta aataatgata ggtacacggt
     2641 ttcgaaggcg gtaatcgaaa ctcgcgctga tctgatcgta tgcttcttca gctacagttg
     2701 tcgcgattct cgctacgcct tccataccgt cgtggaagta gatatcgaaa tggggagtct
     2761 gcacgaactc ccaattgtac attctcgtcg taggtttgtt tcgcccgaag cttccgattt
     2821 gtgccgatac cgggacactg acgagtgtac acgccgtaac gacggtgacg agtaccgatc
//
LOCUS       DSCC01000422            1591 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SpSt-1187
            Ga0129302_1052547, whole genome shotgun sequence.
ACCESSION   DSCC01000422 DSCC01000000
VERSION     DSCC01000422.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639103
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (sediment metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 6.92x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 14:34:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,046
            CDSs (total)                      :: 1,033
            Genes (coding)                    :: 1,032
            CDSs (with protein)               :: 1,032
            Genes (RNA)                       :: 13
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0129302_1052547"
                     /isolate="SpSt-1187"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="39.0314 N 122.4323 W"
                     /collection_date="2014-09-02"
                     /metagenome_source="sediment metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5216255;
                     metagenomic"
     gene            17..1519
                     /locus_tag="ENN56_05230"
     CDS             17..1519
                     /locus_tag="ENN56_05230"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS74929.1"
                     /translation="MNRNPDRPNLLFIFTDEQRFDTLAAYGNSRIDMPNLNRLAERST
                     VFERTYDTQPVCTPARSSIMTGLTPHTNGCVRNNIPLDLNVPTIAEMVPTDGTDGYFT
                     AYHGKWHLGDEIFAQHGFREWISIEDMYRSHYREGRDRTLRSDYHHWLVERGIEPEDG
                     STFTRYETAKLAEEFSKPAFLAENATRFFRERSGDGKPFLLYVNFLEPHTPFFGPRNN
                     QYPPEDVLIPPNFGNPPNAKQPLRQRINARQLLEEGHSDLPLVTEADWRKIIARYWGL
                     CSQVDTHVGRILDALAETGLDESTIVVFTSDHGEMMGSHRLMPKTMMFEESSRIPMLV
                     HLPGQTEQHRVTTPVSQIDLVPTLLDLLNQPKPDHLEGESLADVIRLRKDGTEHRDVF
                     IEWNGRNLGARVPEVKEADLRPYERELGTPQEIARTRAAHQRTVVTPDGWKFSWDEFG
                     EHELYHLSEDPYETQNLAHDTAARGTMRELAERIKNWQKRTGDTVTLATF"
BASE COUNT          391 a          486 c          439 g          275 t
ORIGIN      
        1 gaagggaggg caaccgatga accggaaccc cgaccgcccg aatctccttt tcatcttcac
       61 cgatgaacag cgcttcgaca ctctcgccgc gtacggtaac tctcgaattg acatgccaaa
      121 cctcaatcgg cttgccgaac gaagcacggt cttcgaacgc acgtacgaca cccaaccggt
      181 atgcacaccg gcgcgctcga gcatcatgac cggactgacg ccgcatacaa acggctgcgt
      241 gcggaacaac attcctcttg atctcaatgt accgactatc gccgagatgg tgccgactga
      301 cggtaccgac ggatatttca ccgcatacca cggcaaatgg cacctcggtg acgaaatctt
      361 cgcacaacac ggattccgcg aatggatcag catcgaggat atgtaccgta gccactatcg
      421 cgaaggtcgt gatcgcaccc tgcggtcgga ttaccaccac tggctggtag agcgaggaat
      481 cgaaccggag gatgggagca cgttcactcg ttacgagacc gcgaaactcg ccgaagagtt
      541 cagcaaaccg gcgtttctcg cggagaacgc gacgaggttc tttcgcgaac gctccggcga
      601 tggcaagccg tttctcctct acgtaaactt cctcgagccg cacacgccgt tctttggacc
      661 gcggaacaac caatacccgc cggaagacgt gctgattccg ccgaacttcg gcaatcctcc
      721 aaacgccaag cagccgctcc gtcagcgaat caacgcgcga caactcctcg aagaaggaca
      781 cagtgatctg ccgctggtca cagaagcgga ttggcggaag attatcgcgc gatactgggg
      841 actgtgcagc caggttgaca cgcatgtggg gagaatactc gacgctctcg cggaaaccgg
      901 tctcgacgag agtaccatcg tcgtgttcac ctcggatcac ggtgagatga tgggaagcca
      961 ccgactcatg ccgaagacga tgatgttcga agagtcgtcg cggataccga tgctcgtcca
     1021 tctcccggga cagacggaac agcatcgggt cacaacgccc gtgagtcaga tcgatctcgt
     1081 accgacattg cttgatcttt tgaatcagcc caaacctgat cacctcgaag gcgaaagcct
     1141 tgccgatgtc atccggttga gaaaagacgg tactgagcat cgcgacgtgt tcatcgaatg
     1201 gaacggacgc aatctcggtg cacgagtacc cgaggtgaaa gaggcggact tacgcccgta
     1261 cgagcgggag ttgggaacac cgcaggaaat cgcccgcacc cgcgccgccc accaacgcac
     1321 cgtcgtcacg ccggatgggt ggaagttctc gtgggacgag ttcggcgagc acgaactgta
     1381 tcacctgagc gaggacccgt acgaaacgca gaacctcgct cacgatactg ccgcgcgtgg
     1441 cacaatgcgc gaactggcgg aacggatcaa aaactggcaa aaacgcaccg gcgataccgt
     1501 gacgctcgcg accttctgac gacgtaaatc gacaggctta gtccgcaaac gggaacacta
     1561 cacgtcgtcc cgttcgcgcc gactcgtatg c
//
